Triumphs and Challenges of High-Resolution Mass Spectrometry in Comprehensive Residue Screens
|
|
- Oswald Cobb
- 6 years ago
- Views:
Transcription
1 Triumphs and Challenges of High-Resolution Mass Spectrometry in Comprehensive Residue Screens Florida Department of Agriculture Mark Crosswhite, Ph.D.
2 outline ur Goal identify and quantify quickly and reliably UHPLC v HPLC easy and cost effective improvement ur Two Platforms rbitrap and Triple Quad advantages of high resolution MS on the Exactive high resolution and high mass accuracy allows for post acquisition data mining limitations of high resolution MS on the Exactive cannot separate compounds with same exact masses no mass filtering fragments can be produced from any ions in the cell Resolution elucidate fragment formulas and structures use trusted triple quad fragments even though their identities are often unknown Exactive Plus vs. ABSciex real samples detection quantitation false positive realized Current and Future Strategies
3 outline ur Goal identify and quantify quickly and reliably UHPLC v HPLC easy and cost effective improvement ur Two Platforms rbitrap and Triple Quad advantages of high resolution MS on the Exactive high resolution and high mass accuracy allows for post acquisition data mining limitations of high resolution MS on the Exactive cannot separate compounds with same exact masses no mass filtering fragments can be produced from any ions in the cell Resolution elucidate fragment formulas and structures use trusted triple quad fragments even though their identities are often unknown Exactive Plus vs. ABSciex real samples detection quantitation false positive realized Current and Future Strategies
4 UHPLC Capacities of orthogonal analyses are multiplicative, not additive. Especially helpful with duty cycle issues in MRM MS (triple quad) and high resolution MS where there may not be MS/MS capabilities. propazine vs sebuthylazine m = Dimethomorph (E and Z) Acquity UPLC BEH C18 Cl Cl 1.7 µm 2.1 x 150 mm Flow rate.200 ml/min UHPLC HPLC UHPLC Time (min) Time (min) Time (min)
5 outline ur Goal identify and quantify quickly and reliably UHPLC v HPLC easy and cost effective improvement ur Two Platforms rbitrap and Triple Quad advantages of high resolution MS on the Exactive high resolution and high mass accuracy allows for post acquisition data mining limitations of high resolution MS on the Exactive cannot separate compounds with same exact masses no mass filtering fragments can be produced from any ions in the cell Resolution elucidate fragment formulas and structures use trusted triple quad fragments even though their identities are often unknown Exactive Plus vs. ABSciex real samples detection quantitation false positive realized Current and Future Strategies
6 representation of quadrupole in ms/ms mode Q1 Q2* Q3 mass filters fragments mass measures ion optics representation of rbitrap C-trap HCD cell* Sees what it looks for (MRM) ω=(qk/m) (1/2) orbitrap rbitrap sees everything all the time (full scan) Allows postacquisition data mining * fragments generated by colliding analyte with neutral gas
7 outline ur Goal identify and quantify quickly and reliably UHPLC v HPLC easy and cost effective improvement ur Two Platforms rbitrap and Triple Quad Advantages of High Resolution MS on the Exactive high resolution and high mass accuracy allows for post acquisition data mining limitations of high resolution MS on the Exactive cannot separate compounds with same exact masses no mass filtering fragments can be produced from any ions in the cell Resolution elucidate fragment formulas and structures use trusted triple quad fragments even though their identities are often unknown Exactive Plus vs. ABSciex real samples detection quantitation false positive realized Current and Future Strategies
8 what high resolution MS will do Phosmet [M + H + ] + = RT = min required resolution = m/(δm) Azinphos methyl [M + H + ] + = RT = min S S P S S P Triazophos [M + H + ] + = RT = min required resolution = Hexaconazole [M + H + ] + = RT = min Cl S P H Cl
9 resolving power simazine ( ) carbaryl ( ) RP required = thiabendazole ( ) resolvable with new rbitrap RP required = 4698 carbaryl ( ) m/z m/z
10 m/z m/z simulation v observed of a 1:10 mixture triazophos [M + H + ] + = hexaconazole [M + H + ] + = simulation resolution observed , , ,
11 outline ur Goal identify and quantify quickly and reliably UHPLC v HPLC easy and cost effective improvement ur Two Platforms rbitrap and Triple Quad advantages of high resolution MS on the Exactive rbitrap high resolution and high mass accuracy allows for post acquisition data mining limitations of high resolution MS on the Exactive cannot separate compounds with same exact masses no mass filtering fragments can be produced from any ions in the cell Resolution elucidate fragment formulas and structures use trusted triple quad fragments even though their identities are often unknown Exactive Plus vs. ABSciex real samples detection quantitation false positive realized Current and Future Strategies
12 what high resolution MS alone will not do Secbumeton [M + H + ] + = RT 8.23 min Prometon [M + H + ] + = RT 8.21 min Terbumeton [M + H + ] + = RT 8.32 min Prometryn [M + H + ] + = RT min S Terbutryn [M + H + ] + = RT min S
13 outline ur Goal identify and quantify quickly and reliably UHPLC v HPLC easy and cost effective improvement ur Two Platforms rbitrap and Triple Quad advantages of high resolution MS on the Exactive rbitrap high resolution and high mass accuracy allows for post acquisition data mining limitations of high resolution MS on the Exactive cannot separate compounds with same exact masses no mass filtering fragments can be produced from any ions in the cell Resolution elucidate fragment formulas and structures use trusted triple quad fragments even though their identities are often unknown Exactive Plus vs. ABSciex real samples detection quantitation false positive realized Current and Future Strategies
14 Extracted Ion Chromatogram (upper) Mass Spectra (lower) 8.21 PRMET STADARD Θ * Time (min) V Collision Energy 15 V Collision Energy Θ V Collision Energy * m/z
15 Extracted Ion Chromatogram (upper) Mass Spectra (lower) 8.32 TERBUMET STADARD Θ * Time (min) V Collision Energy 15 V Collision Energy Θ V Collision Energy * m/z
16 trusted triple quad fragments real detect.040 ug/g ABSciex 5500 azoxystrobin frag intensity (arb) azoxystrobin frag ug/g 0 V collision energy 15 V collision energy 45 V collision energy Exactive Plus Time extracted ion chromatogram azoxystrobin + H + extracted ion chromatogram mass = extracted ion chromatogram mass =
17 outline ur Goal identify and quantify quickly and reliably UHPLC v HPLC easy and cost effective improvement ur Two Platforms rbitrap and Triple Quad advantages of high resolution MS on the Exactive rbitrap high resolution and high mass accuracy allows for post acquisition data mining limitations of high resolution MS on the Exactive cannot separate compounds with same exact masses no mass filtering fragments can be produced from any ions in the cell Resolution elucidate fragment formulas and structures use trusted triple quad fragments even though their identities are often unknown Exactive Plus vs. ABSciex real samples detection quantitation false positive realized Current and Future Strategies
18 B 3688 compound matrix ABSciex 5500 quantitation (μg/g) Exactive plus quantitation (μg/g) percent difference exact mass (H+ adduct, Da) measured mass (Da) mass error (ppm) azoxystrobin eggplant pyraclostrobin blueberry dimethoate blueberry thiabendazole pear pyrimethanil strawberry flonicamid strawberry fluopicolide onion azoxystrobin onion thiabendazole apple imazalil banana
19 B 3704 compound matrix ABSciex 5500 quantitation (μg/g) Exactive plus quantitation (μg/g) percent difference exact mass (H+ adduct, Da) measured mass (Da) mass error (ppm) methamidophos collards * acephate collards dinotefuran collards thiabendazole papaya chlorantraniliprole collards chlorantraniliprole peas azoxystrobin papaya boscalid blackberries boscalid papaya * possible suppression in source or interference in mass measurement
20 B 3706 compound matrix ABSciex Exactive 5500 plus quantitation quantitation (μg/g) (μg/g) percent difference exact mass (H+ adduct, Da) measured mass (Da) mass error (ppm) methamidophos acephate bell peppers bell peppers * carbendazim okra azoxystrobin bell peppers azoxystrobin bell peppers boscalid tomato methoxyfenozide bell peppers bifenazate bell peppers difenoconazole bell peppers * remember the extracted ion chromatograms using trusted triple quad fragments
21 B 3707 compound matrix ABSciex Exactive 5500 plus quantitation quantitation (μg/g) (μg/g) percent difference exact mass (H+ adduct, Da) measured mass (Da) mass error (ppm) phorate sulfoxide potatoe trifloxystrobin strawberry pyrimethanil strawberry novaluron strawberry propamocarb squash pyraclostrobin bell pepper pyraclostrobin tomato pyraclostrobin strawberry penthiopyrad bean propamocarb cucumber
22 propoxur (internal control) from B 3707 measured mass of propoxur +H + ppm error average error
23 summary information of real samples f ~ 125 samples screened for ~ 250 pesticides per sample on the Exactive Plus we found: no false positives when compared to ms/ms analysis on an ABSciex 5500 (everything confirmed) no false negatives when compared to an ABSciex 5500 (Exactive Plus did not miss anything at the established LDs) the largest percent difference and average percent difference in quantitation between the Exactive Plus and the ABSciex 5500 was 60 and 4 respectively. one false positive on the ABSciex 5500 (not their fault)
24 real sample false positive realized 302.1/97.2 fenhexamid B3716_R1_01_57782 intensity (arb) 302.1/55.0 fenhexamid B3716_R1_01_ E E 6 extracted ion chromatogram of fenhexamid +H + on Exactive Plus B 3716_R1_01_57782 Time
25 outline ur Goal identify and quantify quickly and reliably UHPLC v HPLC easy and cost effective improvement ur Two Platforms rbitrap and Triple Quad advantages of high resolution MS on the Exactive rbitrap high resolution and high mass accuracy allows for post acquisition data mining limitations of high resolution MS on the Exactive cannot separate compounds with same exact masses no mass filtering fragments can be produced from any ions in the cell Resolution elucidate fragment formulas and structures use trusted triple quad fragments even though their identities are often unknown Exactive Plus vs. ABSciex real samples detection quantitation false positive realized Current and Future Strategies
26 our current strategy and flow if no detections screen with Exactive Plus for ~250 compounds if analyte found above LD report report using ABSciex 5500 identification and quantitation using ms/ms, ion ratios and a 6 point calibration curve
27 our next strategy and flow if analyte found at <.5 tolerance screen with Exactive Plus for ~500 compounds if analyte found at >.5 tolerance report report using ABSciex 5500 estimate and characterization- 1 point calibration standard, and characteristic fragments for confirmation identification and quantitation using ms/ms, ion ratios and a 6 point calibration curve
28 our following strategy and flow if analyte found at < tolerance screen with Exactive Plus for ~750 compounds if analyte found at > tolerance Exactive Plus report using ABSciex 5500 quantitation and characterization- 6 point calibration curve, and characteristic fragments for confirmation identification and quantitation using ms/ms, ion ratios and a 6 point calibration curve
29 our ideal strategy and flow routine screen with Exactive Plus for ~1000 compounds if needed/special case Exactive Plus report using ABSciex 5500 quantitation and characterization- 6 point calibration curve, and characteristic fragments for confirmation identification and quantitation using ms/ms, ion ratios and a 6 point calibration curve
30 acknowledgements Walter Hammack Ghislain Gerard Casey Burrows Erin McAllister Jon Pelt
Overview. Introduction
Comprehensive Quantitation and Identification of Pesticides in Food Samples Using the SCIEX UltraLC 100 and the SCIEX QTRAP 4500 System André Schreiber SCIEX, Concord, Ontario, Canada Overview Liquid Chromatography
More informationAdvanced Data Acquisition and Data Processing Workflows to Identify, Quantify and Confirm Pesticide Residues
Advanced Data Acquisition and Data Processing Workflows to Identify, Quantify and Confirm Pesticide Residues André Schreiber 1 and Lauryn Bailey 2 1 AB SCIEX Concord, Ontario (Canada); 2 AB SCIEX Framingham,
More informationCharles Yang, Dipankar Ghosh, Jonathan Beck Thermo Fisher Scientific, San Jose, CA, USA
Utilization of High Resolution LC-MS for Screening and Quantitative Analysis of Pesticides in Food Matrix using a Q Exactive Benchtop Orbitrap Platform Charles Yang, Dipankar Ghosh, Jonathan Beck Thermo
More informationFood & Environmental. Overview. Introduction. Method Details. André Schreiber and Yun Yun Zou SCIEX, Concord, Ontario, Canada
Food & Environmental Comprehensive Quantitation and Identification of Pesticides in Food Samples using LC-MS/MS with Scheduled MRM, Fast Polarity Switching, and MS/MS Library Searching André Schreiber
More informationApplying MRM Spectrum Mode and Library Searching for Enhanced Reporting Confidence in Routine Pesticide Residue Analysis
PO-CON1768E Applying MRM Spectrum Mode and Library Searching for Enhanced Reporting Confidence in Routine Pesticide ASMS 2017 TP-194 David Baker 1, Christopher Titman 1, Neil Loftus 1, Jonathan Horner
More informationDETERMINATION OF PESTICIDES IN FOOD USING UPLC WITH POLARITY SWITCHING TANDEM QUADRUPOLE LC/MS/MS
DETERMINATION OF PESTICIDES IN FOOD USING UPLC WITH POLARITY SWITCHING TANDEM QUADRUPOLE LC/MS/MS Peter Hancock 1, Cristiana C. Leandro 2,3, Brendan J. Keely 2 and Richard J. Fussell 3 1 Waters Corporation,
More informationOverview. Introduction. André Schreiber 1 and Yun Yun Zou 1 1 AB SCIEX, Concord, Ontario, Canada
LC-MS/MS Based Strategy for the Non-Targeted Screening of an Unlimited Number of Contaminants in Food Using the AB SCIEX TripleTOF 5600 System and Advanced Software Tools André Schreiber 1 and Yun Yun
More informationOverview. Introduction. Method Details. André Schreiber and Yun Yun Zou AB SCIEX, Concord, Ontario, Canada
Comprehensive Quantitation and Identification of Pesticides in Food Samples using LC-MS/MS with Scheduled MRM, Fast Polarity Switching, and MS/MS Library Searching André Schreiber and Yun Yun Zou AB SCIEX,
More informationRapid Pesticide Screening and Identification Using the High Resolution All Ions MS/MS Technique
Rapid Pesticide Screening and Identification Using the High Resolution All Ions MS/MS Technique Application Note Food Safety Authors Bernhard Wüst and Thomas Glauner Agilent Technologies GmbH Waldbronn
More informationDetermination of pesticides in baby food by UHPLC/MS/MS using the Agilent 1290 Infinity LC system and the Agilent 6460 triple quadrupole LC/MS
Determination of pesticides in baby food by UHPLC/MS/MS using the Agilent 1290 Infinity LC system and the Agilent 6460 triple quadrupole LC/MS Application Note Food Authors Gerd Vanhoenacker, Frank David,
More informationUltra-fast screening of pesticides in foods and agricultural products with desorption corona beam ionization (DCBI) tandem mass spectrometry
PO-CON18E Ultra-fast screening of pesticides in foods and agricultural products with desorption corona beam ionization (DCBI) ASMS 21 TP 3 Jing Dong, Satoshi Yamaki, Yuki Hashi, Naoki Hamada Shimadzu China,
More informationMultiresidue Analysis of 100 Pesticides in Food Samples by LC/Triple Quadrupole Mass Spectrometry Application
Multiresidue Analysis of Pesticides in Food Samples by LC/Triple Quadrupole Mass Spectrometry Application Food Safety Authors Imma Ferrer and E. Michael Thurman Pesticide Residue Research Group University
More informationHigher Confidence in Identification with QTRAP LC/MS/MS Systems when Screening and Quantifying Pesticides in Fruit and Vegetable Samples
Higher Confidence in Identification with QTRAP LC/MS/MS Systems when Screening and Quantifying Pesticides in Fruit and Vegetable Samples André Schreiber AB SCIEX, Concord, Ontario, Canada Figure 1. Ion
More informationYun W. Alelyunas, Mark D. Wrona, Russell J. Mortishire-Smith, Nick Tomczyk, and Paul D. Rainville Waters Corporation, Milford, MA, USA INTRODUCTION
Quantitation by High Resolution Mass Spectrometry: Using Target Enhancement and Tof-MRM to Achieve Femtogram-level On-column Sensitivity for Quantitation of Drugs in Human Plasma Yun W. Alelyunas, Mark
More informationMulti-residue analysis of pesticides by GC-HRMS
An Executive Summary Multi-residue analysis of pesticides by GC-HRMS Dr. Hans Mol is senior scientist at RIKILT- Wageningen UR Introduction Regulatory authorities throughout the world set and enforce strict
More informationQuan/Qual Analyses. Unmatched Confidence for. Thermo Scientific Q Exactive Orbitrap LC-MS/MS System. Identify Quantify Confirm
26.1645 165.91 33.267 Thermo Scientific Q Exactive rbitrap LC-MS/MS System Unmatched Confidence for Quan/Qual Analyses Identify Quantify Confirm Quanfirmation \ kwän-f r-'m -shän\ n 1 : the ability to
More informationQuantitation of High Resolution MS Data Using UNIFI: Acquiring and Processing Full Scan or Tof-MRM (Targeted HRMS) Datasets for Quantitative Assays
: Acquiring and Processing Full Scan or Tof-MRM (Targeted HRMS) Datasets for Quantitative Assays Mark Wrona, Jayne Kirk, and Yun Alelyunas Waters Corporation, Milford, MA, USA APPLICATION BENEFITS Ability
More informationTheory and Practical Consequences.
Theory and Practical Consequences. Number of hits depending on reachable mass accuracy 250 hits @ ±10 ppm 137 hits @ ±5 ppm 78 hits @ ±3 ppm 29 hits @ ±1 ppm 3 hits @ ±0.1 ppm 1 hit @ ± 0.05 ppm Intens.
More informationAnalysis of Pesticides in Citrus Oil Using a Shimadzu GCMS-TQ8030. No. GCMS No. SSI-GCMS-1404
Gas Chromatograph Mass Spectrometer No. GCMS-1404 Analysis of Pesticides in Citrus Oil Using a Shimadzu GCMS-TQ8030 Introduction Contamination of consumer products with pesticides is a growing concern
More informationPesticideScreener. Innovation with Integrity. Comprehensive Pesticide Screening and Quantitation UHR-TOF MS
PesticideScreener Comprehensive Pesticide Screening and Quantitation Innovation with Integrity UHR-TOF MS The Challenge of Comprehensive Pesticide Residue Analysis The use of pesticides to reduce crop
More informationTOMAHAQ Method Construction
TOMAHAQ Method Construction Triggered by offset mass accurate-mass high-resolution accurate quantitation (TOMAHAQ) can be performed in the standard method editor of the instrument, without modifications
More informationPesticides in Cannabis
LCMS-8040 Application Pesticides in Cannabis Jeff Dahl, 1 Julie Kowalski, 2 Jason Zitzer, 3 and Gordon Fagras 3 1 Shimadzu Scientific Instruments; Columbia, Maryland 2 Restek Corporation; Bellefonte, Pennsylvania
More informationdynamic MRM for pesticide analysis
The power of fast separation and dynamic MRM for pesticide analysis based on 1290/6460QQQ Dr. Volker Gnau Produkt Spezialist LCMS Page 1 3.) Quantitative analysis of pesticides (smrm) Chromatographic hi
More informationLC/MS/MS Analysis of Pesticide Residues in Apples Using Agilent Chem Elut Cartridges
LC/MS/MS Analysis of Pesticide Residues in Apples Using Agilent Chem Elut Cartridges Application ote Food Testing & Agriculture Author Andy Zhai Agilent Technologies Shanghai Co. Ltd. Abstract This application
More informationWADA Technical Document TD2003IDCR
IDENTIFICATION CRITERIA FOR QUALITATIVE ASSAYS INCORPORATING CHROMATOGRAPHY AND MASS SPECTROMETRY The appropriate analytical characteristics must be documented for a particular assay. The Laboratory must
More informationSimplified Workflow and Improved Confidence in Screening Methods on Time of Flight Mass Spectrometry
Simplified Workflow and Improved Confidence in Screening Methods on Time of Flight Mass Spectrometry Steve Preece MS Business Manager Waters Wilmslow 2016 Waters Corporation 1 Overview Use of high resolution
More informationAnalysis of Pesticides in Baby Foods Using a Triple-Quadrupole GC/MS/MS
PO-CONE Analysis of Pesticides in Baby Foods Using a Pittcon 01 -P Laura Chambers, Richard Whitney, Ph.D., Nicole M. Lock, Zhuangzhi Max Wang, Ph.D., Clifford M. Taylor; Shimadzu Scientific Instruments,
More informationPure Chromatography. Food Safety Applications. Abstract. Introduction
Food Safety Applications Mitigating Matrix Effects: Examination of Dilution, QuEChERS, and Calibration Strategies for LC-MS/MS Analysis of Pesticide Residues in Diverse Food Types Abstract By Julie Kowalski,
More informationAnalysis of Pesticides and Contaminants using HS-SPME-GC/MS and DRS Detection. Blair Surridge, Tyler Dyer, Rene Bilideau, Paloma Bertolin Presoti
Analysis of Pesticides and Contaminants using HS-SPME-GC/MS and DRS Detection Blair Surridge, Tyler Dyer, Rene Bilideau, Paloma Bertolin Presoti Applied Research at Camosun College Provides funding to
More information2016 iquan Series: Pesticide Quantitation on the Thermo Scientific TSQ Quantiva
2016 iquan Series: Pesticide Quantitation on the Thermo Scientific TSQ Quantiva Craig Dufresne and Kevin McHale, Content Creators The world leader in serving science The Whole Workflow Efficiency in sample
More informationComprehensive Pesticide Analysis in Juice Using a Combination of GC/MS and LC/MS Methods
Comprehensive Pesticide Analysis in Juice Using a Combination of GC/MS and LC/MS Methods Application Note Food Safety Authors Fadwa Al-Taher, Rima Juskelis, Yang Chen, Jack Cappozzo Institute for Food
More informationMulti-residue GC-MS analysis. Richard Fussell CSL York, UK
Multi-residue GC-MS analysis Richard Fussell CSL York, UK e-mail: r.fussell@csl.gov.uk Outline of Presentation Background Gas Chromatography Mass Spectrometry - Options Capabilities and Applications Summary
More informationApplication News. No. C154. Applying MRM Spectrum Mode and Library Searching for Enhanced Reporting Confidence in Routine Pesticide Residue Analysis
LAAN-A-LM-E124 Application Liquid Chromatography Mass Spectrometry Applying MRM Spectrum Mode and Library Searching for Enhanced Reporting Confidence in Routine Pesticide Residue Analysis David R. Baker
More informationLC-HRMS: Challenges for Routine Implementation
Joint EURL pesticides meeting October 23 rd -25th, 2013 Almeria Spain LC-HRMS: Challenges for Routine Implementation Richard J. Fussell Food and Environment Research Agency (York, UK) www.fera.defra.gov.uk
More informationLC-MS/MS multiresidue method used for pesticides analysis in fruits and vegetables
LC-MS/MS multiresidue method used for pesticides analysis in fruits and vegetables 1st International Symposium on Recent Advances in Food Analysis 5-7 ovember 2003, Prague Didier RTELLI, Patrick EDDER,
More informationDate ACCURATE-MASS DATABASE FOR SCREENING OF PESTICIDE RESIDUES IN FRUITS AND VEGETABLES BY GAS CHROMATOGRAPHY TIME-OF-FLIGHT MASS SPECTROMETRY
Date ACCURATE-MASS DATABASE FOR SCREENING OF PESTICIDE RESIDUES IN FRUITS AND VEGETABLES BY GAS CHROMATOGRAPHY TIME-OF-FLIGHT MASS SPECTROMETRY In practice, target analysis approach (analysing 1-4 of GC
More informationNew Dynamic MRM Mode Improves Data Quality and Triple Quad Quantification in Complex Analyses
New Dynamic MRM Mode Improves Data Quality and Triple Quad Quantification in Complex Analyses Technical Overview Authors Abstract Peter Stone, Thomas Glauner, Frank Kuhlmann, Tim Schlabach and Ken Miller
More informationTheory of Sampling. Guide to Quality Sample Processing. Jo Marie Cook NACRW 2015
Theory of Sampling Guide to Quality Sample Processing Jo Marie Cook NACRW 2015 2011 Food Safety Modernization Act Integrated Food Safety System Section 202(a)(6): Standards for Sampling and Testing Partnership
More informationTypes of Analyzers: Quadrupole: mass filter -part1
16 Types of Analyzers: Sector or double focusing: magnetic and electric Time-of-flight (TOF) Quadrupole (mass filter) Linear ion trap Quadrupole Ion Trap (3D trap) FTICR fourier transform ion cyclotron
More informationAnalytical Test Report 10
OLCC #022892C 9 SE Division St. Laboratory ID: 805024-02 Batch ID: AC Date Sampled: 05/07/8 Date Printed: 05//8 Report cannot be used for OLCC/OHA compliance. Date Sampled: 05/07/8 00:00 Date Accepted:
More informationLászló Tölgyesi LC/MS scientist EFSC Demo Group. International Symposium on Food Safety and Quality: Applications of Nuclear and Related Techniques
Minimizing matrix effects in multiresidue pesticide analysis using a new sensitivity-enhanced triple quadrupole instrument in combination with extensive sample dilution László Tölgyesi LC/MS scientist
More informationScreening Validation Report 1
EURL for Cereals and Feeding stuff National Food Institute Technical University of Denmark Appendix 4 Screening Validation Report 1 Screening of pesticide residues in cereals by UPLC-TOF (QuEChERS method)
More informationLC/Q-TOF Workflows for Comprehensive Micropollutant Analysis
LC/Q-TO Workflows for Comprehensive Micropollutant Analysis Targeted Quantification, Suspect Screening, and Unknown Compound Identification Application Note Environmental Authors Christoph Moschet and
More informationAB SCIEX SelexION Technology Used to Improve Mass Spectral Library Searching Scores by Removal of Isobaric Interferences
AB SCIEX SelexION Technology Used to Improve Mass Spectral Library Searching s by Removal of Isobaric Interferences Differential Mobility Used as a Tool to Address Selectivity Challenges Adrian M. Taylor
More informationLiquid Chromatography Mass Spectrometry. Expanding Capabilities in Multi-Residue Pesticide Analysis Using The LCMS-8060
LAAN-A-LM-E107 Application Liquid Chromatography Mass Spectrometry Expanding Capabilities in Multi-Residue Pesticide Analysis Using The LCMS-8060 David R. Baker 1, Laëtitia Fages 2, Eric Capodanno 2, Neil
More informationApplication Note LCMS-110 Development of a Targeted Quantitative LC-MS/MS Method for 431 Positive and Negative Ion Pesticides in a Single Analysis
Application Note LCMS-110 Development of a Targeted Quantitative LC-MS/MS Method for 431 Positive and Negative Ion Pesticides in a Single Analysis Abstract A rapid, targeted quantitative UHPLC-triple quadrupole
More informationIncreasing Speed of UHPLC-MS Analysis Using Single-stage Orbitrap Mass Spectrometer
Increasing Speed of UHPLC-MS Analysis Using Single-stage Orbitrap Mass Spectrometer Olaf Scheibner and Maciej Bromirski Thermo Fisher Scientific, Bremen, Germany Overview Purpose: Improve the performance
More informationTyler Trent, Applications Sales Specialist, Teledyne Tekmar P a g e 1
Application Note AutoMate-Q40 Automated QuEChERS Extraction for Pesticide Residues in Botanicals Tyler Trent, Applications Sales Specialist, Teledyne Tekmar P a g e 1 Abstract QuEChERS is a Quick-Easy-Cheap-
More informationOverview. Introduction. André Schreiber AB SCIEX Concord, Ontario (Canada)
Quantitation and Identification of Pharmaceuticals and Personal Care Products (PPCP) in Environmental Samples using Advanced TripleTOF MS/MS Technology André Schreiber AB SCIEX Concord, Ontario (Canada)
More informationUtility of H-SRM to Reduce Matrix Interference in Food Residue Analysis of Pesticides by LC-MS/MS Using the TSQ Quantum Discovery
Application Note: 3 Utility of H-SRM to Reduce Matrix Interference in Food Residue Analysis of Pesticides by LC-MS/MS Using the TSQ Quantum Discovery Yoko Yamagishi, Thermo Fisher Scientific, C-2F 3-9
More informationImproved Screening for 250 Pesticides in Matrix using a LC-Triple Quadrupole Mass Spectrometer
Improved Screening for 2 Pesticides in Matrix using a LC-Triple Quadrupole Mass Spectrometer Mary lackburn, Jia Wang, Jonathan eck, Charles Yang, Dipankar Ghosh, Thermo Fisher Scientific, San Jose, C,
More informationSimplified Approaches to Impurity Identification using Accurate Mass UPLC/MS
Simplified Approaches to Impurity Identification using Accurate Mass UPLC/MS Marian Twohig, Michael D. Jones, Dominic Moore, Peter Lee, and Robert Plumb Waters Corporation, Milford, MA, USA APPLICATION
More informationQuantitative and Qualitative Confirmation of Pesticides in Beet Extract Using a Hybrid Quadrupole-Orbitrap Mass Spectrometer
Quantitative and Qualitative Confirmation of Pesticides in Beet Extract Using a Hybrid Quadrupole-Orbitrap Mass Spectrometer Charles Yang and Dipankar Ghosh, Thermo Fisher Scientific, San Jose, CA Olaf
More informationAn Effective Workflow for Impurity Analysis Incorporating High Quality HRAM LCMS & MSMS with Intelligent Automated Data Mining
An Effective Workflow for Impurity Analysis Incorporating High Quality HRAM LCMS & MSMS with Intelligent Automated Data Mining Dave Weil, Ph.D. and Jim Lau, Ph.D. Typical Method Conditions: 1260 UHPLC
More informationTHE IMPLEMENTATION OF A SCREENING WORKFLOW FOR ION MOBILITY QUADRUOPOLE TIME-OF-FLIGHT MASS SPECTROMETRIC ANALYSIS OF PFOS ISOMERS
THE IMPLEMENTATION O A SCREENING WORKLOW OR ION MOBILITY QUADRUOPOLE TIME-O-LIGHT MASS SPECTROMETRIC ANALYSIS O POS ISOMERS Ken Rosnack ken_rosnack@waters.com Ingrid Ericson Jogsten and Bert van Bavel
More informationThe Emergence of Benchtop Tandem Quadrupole Mass Spectrometers as a Routine Tool in Quantitative GC/MS/MS Applications
The Emergence of Benchtop Tandem Quadrupole Mass Spectrometers as a Routine Tool in Quantitative GC/MS/MS Applications Doug Stevens Waters Corp. Introduction The range of GC/MS instrument types has expanded
More informationIdentification and Characterization of an Isolated Impurity Fraction: Analysis of an Unknown Degradant Found in Quetiapine Fumarate
Identification and Characterization of an Isolated Impurity Fraction: Analysis of an Unknown Degradant Found in Quetiapine Fumarate Michael D. Jones, Xiang Jin Song, Robert S. Plumb, Peter J. Lee, and
More informationMultiple Fragmentation Methods for Small Molecule Characterization on a Dual Pressure Linear Ion Trap Orbitrap Hybrid Mass Spectrometer
Application ote: 54 Multiple Fragmentation Methods for Small Molecule Characterization on a Dual Pressure Linear Ion Trap rbitrap Hybrid Mass Spectrometer Kate Comstock, Yingying Huang; Thermo Fisher Scientific,
More informationScreening of pesticides residues by the time of flight analyzer (ToF) : Myth or reality?
Screening of pesticides residues by the time of flight analyzer (ToF) : Myth or reality? Laure Joly & Vincent Hanot WIV-ISP, Juliette Wytsmanstraat 14, 15 Brussel The passengers pesticides are requested......
More informationQ-TOF PREMIER DYNAMIC RANGE ENHANCEMENT AND ACCURATE MASS MEASUREMENT PERFORMANCE
Q-TOF PREMIER DYAMIC RAGE EHACEMET AD ACCURATE MASS MEASUREMET PERFORMACE Michael McCullagh, Jose Castro-Perez, and Lisa Calton Waters Corporation, Manchester, UK ITRODUCTIO The use of time-of-flight (TOF)
More informationDetermination of Pesticide Residues in Oats by Automated. QuEChERS and LC/QQQ. Application Note. Abstract. Introduction
Determination of Pesticide Residues in Oats by Automated QuEChERS and LC/QQQ Application Note Abstract The QuEChERS (Quick-Easy-Cheap-Effective-Rugged-Safe) sample extraction method was developed for the
More informationApplication of a Triggered MRM Database and Library for the Quantitation and Identification of Pesticides in Food Extracts
Application of a Triggered MRM Database and Library for the Quantitation and Identification of Pesticides in Food Extracts Application Note Food Authors Thomas Glauner Agilent Technologies, Inc. Waldbronn,
More informationRapid and Accurate Forensics Analysis using High Resolution All Ions MS/MS
Rapid and Accurate Forensics Analysis using High Resolution All Ions MS/MS Application Note Forensic Toxicology Authors Martin Josefsson, and Markus Roman National Board of Forensic Medicine Linköping,
More informationDetermination of Pesticide Multiresidues in Apple, Pear and Grape using modified QuEChERS and analysis by LC-QTOF MS
Determination of Pesticide Multiresidues in Apple, Pear and Grape using modified QuEChERS and analysis by LC-QTOF MS Application Note Authors Renato Zanella, Juliana S. Munaretto, and Manoel L. Martins
More informationAnalytical Technologies and Compound Identification. Daniel L. Norwood, MSPH, PhD SCĪO Analytical Consulting, LLC.
Analytical Technologies and Compound Identification Daniel L. Norwood, MSPH, PhD SCĪ Analytical Consulting, LLC. Extractables/Leachables Characterization (USP) Characterization is the discovery,
More informationMass Spectrometry. Hyphenated Techniques GC-MS LC-MS and MS-MS
Mass Spectrometry Hyphenated Techniques GC-MS LC-MS and MS-MS Reasons for Using Chromatography with MS Mixture analysis by MS alone is difficult Fragmentation from ionization (EI or CI) Fragments from
More informationApplication Note. Abstract. Authors. Food Safety and Environmental
Multi-Residue Pesticide Analysis with Dynamic Multiple Reaction Monitoring and Triple Quadrupole LC/MS/MS Fast and Effective Method Development Using an Application Kit and a Pesticides Compound Parameter
More informationHigh Efficiency, Broad Scope Screening of Pesticides Using Gas Chromatography High Resolution Orbitrap Mass Spectrometry
High Efficiency, Broad Scope Screening of Pesticides Using Gas Chromatography High Resolution Orbitrap Mass Spectrometry Dominic Roberts,1 Hans Mol,2 Marc Tienstra,2 Cristian Cojocariu,1 and Paul Silcock1
More informationQualitative and Quantitative Analysis of Pesticides in Horse Feed Matrix Using Orbitrap MS
Qualitative and Quantitative Analysis of esticides in Horse Feed Matrix Using rbitrap M laf cheibner and Maciej Bromirski, Thermo Fisher cientific, Bremen, Germany Application Note 573 Key Words Exactive
More informationPowerful Scan Modes of QTRAP System Technology
Powerful Scan Modes of QTRAP System Technology Unique Hybrid Triple Quadrupole Linear Ion Trap Technology Provides Powerful Workflows to Answer Complex Questions with No Compromises While there are many
More informationIntroduction to the Q Trap LC/MS/MS System
www.ietltd.com Proudly serving laboratories worldwide since 1979 CALL +1.847.913.0777 for Refurbished & Certified Lab Equipment ABI Q Trap LC/MS/MS Introduction to the Q Trap LC/MS/MS System The Q Trap
More informationTargetScreener. Innovation with Integrity. A Comprehensive Screening Solution for Forensic Toxicology UHR-TOF MS
TargetScreener A Comprehensive Screening Solution for Forensic Toxicology Innovation with Integrity UHR-TOF MS TargetScreener Get the Complete Picture Forensic laboratories are frequently required to perform
More informationAgilent ESI and APCI sources: for polar to non-polar compounds
1 Agilent 6400 Series Triple Quadrupole Users Workshop 1 Agilent ESI and APCI sources: for polar to non-polar compounds Nebulizer Pressure Corona current Nebulizer Pressure Vaporizer Vcap Vcap Drying Gas
More informationTANDEM MASS SPECTROSCOPY
TANDEM MASS SPECTROSCOPY 1 MASS SPECTROMETER TYPES OF MASS SPECTROMETER PRINCIPLE TANDEM MASS SPECTROMETER INSTRUMENTATION QUADRAPOLE MASS ANALYZER TRIPLE QUADRAPOLE MASS ANALYZER TIME OF FLIGHT MASS ANALYSER
More informationMassHunter TOF/QTOF Users Meeting
MassHunter TOF/QTOF Users Meeting 1 Qualitative Analysis Workflows Workflows in Qualitative Analysis allow the user to only see and work with the areas and dialog boxes they need for their specific tasks
More informationFully automated QuEChERS clean-up and LC/MS-QQQ analysis of pesticides in fruits and vegetables.
Chromatography Technical Note No AS90 Fully automated QuEChERS clean-up and LC/MS-QQQ analysis of pesticides in fruits and vegetables. Paul H. Roberts, Anatune Ltd. Hardwick, Cambridgeshire, UK. Introduction
More informationRoutine Multiresidue Pesticide Analysis using the Agilent 6470 Triple Quadrupole Mass Spectrometer
Routine Multiresidue Pesticide Analysis using the Agilent 6470 Triple Quadrupole Mass Spectrometer Application Note Food safety Authors Mark Sartain and Anabel Fandino Agilent Technologies, Inc. Santa
More information5Cl-AKB48 and 5Br-AKB48
NMS Labs 2300 Stratford Ave Willow Grove, PA 19090 5Cl-AKB48 and 5Br-AKB48 5Cl-AKB48 Sample Type: Seized Material Latest Revision: May 18 th, 2018 Date Received: February 16 th, 2018 Date of Report: April
More informationMetabolomics in an Identity Crisis? Am I a Feature or a Compound? The world leader in serving science
Metabolomics in an Identity Crisis? Am I a Feature or a Compound? The world leader in serving science Agenda 1 2 3 Unknown Analysis in Metabolomics Determining Features and Compounds for Data Reduction
More informationScreening and Quantitation of 240 Pesticides in Difficult Food Matrixes Using the Agilent 6545 Q-TOF LC/MS System
Screening and Quantitation of 24 Pesticides in Difficult Food Matrixes Using the Agilent 6545 Q-TOF LC/MS System Application Note Authors Dan-Hui Dorothy Yang, Christian Klein, Crystal Cody, and Huy Bui
More informationAnaylsis of Pesticide Residues in Rice Using Agilent Bond Elut QuEChERS AOAC Kit by LC-MS/MS Detection
Anaylsis of Pesticide Residues in Rice Using Agilent Bond Elut QuEChERS AOAC Kit by LC-MS/MS Detection Application Note Food Safety Author Andy Zhai Agilent technologies Co., Ltd. 412 Yinglun Road Shanghai,
More informationQTRAP Technology A COMPENDIUM OF RELATED RESEARCH APPLICATIONS
QTRAP Technology A COMPENDIUM OF RELATED RESEARCH APPLICATIONS QTRAP Technology Discover QTRAP, the only triple quadrupole technology in the world powered by a linear ion trap (LIT), combining the MRM
More informationCerno Application Note Extending the Limits of Mass Spectrometry
June Cerno Application Note Extending the Limits of Mass Spectrometry Accurate Mass Compound Identification with Single Quadrupole GC/MS Yongdong Wang I and Harry Prest II Single Quad GC/MS instruments
More informationKey Words Q Exactive, Accela, MetQuest, Mass Frontier, Drug Discovery
Metabolite Stability Screening and Hotspot Metabolite Identification by Combining High-Resolution, Accurate-Mass Nonselective and Selective Fragmentation Tim Stratton, Caroline Ding, Yingying Huang, Dan
More information2010 Waters Corporation. Xevo TQ-S for Bioanalysis
Xevo TQ-S for Bioanalysis Key innovations High Performance Stepwave RADAR Scan Wave Versatility Ion Sources Simplicity of Operation Engineered Simplicity The Xevo TQ family Built on the same foundations
More informationSensitive and Repeatable Analysis of Pesticides in QuEChERS Extracts with APGC-MS/MS
Sensitive and Repeatable Analysis of Pesticides in QuEChERS Extracts with APGC-MS/MS Dominic Roberts, 1 Sara Stead, 1 and Jennifer A. Burgess 2 1 Waters Corporation, Manchester, UK 2 Waters Corporation,
More informationWADA Technical Document TD2015IDCR
MINIMUM CRITERIA FOR CHROMATOGRAPHIC-MASS SPECTROMETRIC CONFIRMATION OF THE IDENTITY OF ANALYTES FOR DOPING CONTROL PURPOSES. The ability of a method to identify an analyte is a function of the entire
More informationAnalysis of Polar Metabolites using Mass Spectrometry
Analysis of Polar Metabolites using Mass Spectrometry TransMed Course: Basics in Clinical Proteomics and Metabolomics. Oct 10-19, 2012 dd.mm.yyyy Vidya Velagapudi, Ph.D, Adjunct Professor Head of the Metabolomics
More informationImproved 6- Plex TMT Quantification Throughput Using a Linear Ion Trap HCD MS 3 Scan Jane M. Liu, 1,2 * Michael J. Sweredoski, 2 Sonja Hess 2 *
Improved 6- Plex TMT Quantification Throughput Using a Linear Ion Trap HCD MS 3 Scan Jane M. Liu, 1,2 * Michael J. Sweredoski, 2 Sonja Hess 2 * 1 Department of Chemistry, Pomona College, Claremont, California
More informationChoosing the metabolomics platform
GBS 748 Choosing the metabolomics platform Stephen Barnes, PhD 4 7117; sbarnes@uab.edu So, I have my samples what s next? You ve collected your samples and you may have extracted them Protein precipitation
More informationAll Ions MS/MS: Targeted Screening and Quantitation Using Agilent TOF and Q-TOF LC/MS Systems
All Ions MS/MS: Targeted Screening and Quantitation Using Agilent TOF and Q-TOF LC/MS Systems Technical Overview Introduction All Ions MS/MS is a technique that is available for Agilent high resolution
More informationAnalytical determination of testosterone in human serum using an Agilent Ultivo Triple Quadrupole LC/MS
Application Note Clinical Research Analytical determination of testosterone in human serum using an Agilent Ultivo Triple Quadrupole LC/MS Authors Yanan Yang 1, Victor Mandragon 2, and Peter Stone 1 1
More informationDetermination of 198 pesticide residues in eggplant using Gas Chromatography tandem Mass Spectrometry/Mass Spectrometry
PO-CON1619E Determination of 198 pesticide residues in eggplant using Gas Chromatography tandem Mass Spectrometry/Mass Spectrometry Pittcon 2016 480-25 Yan Wang, Feifei Tian, Yueqi Li, Guixiang Yang, Taohong
More informationAnalytical Test Report Birthday Cake OG
ORELAP #4096 OLCC #1002112892C Science First. 11119 SE Division St. Portland, OR 97266 (503) 307-0096 Analytical Test Report Birthday Cake OG Gardens of Odin 020-1003851CB2B Laboratory ID: 1710112-03 Batch
More informationTomorrow s quantitation with the TSQ Fortis mass spectrometer: quantitation of phenylephrine hydrochloride for QA/QC laboratories
APPLICATION NOTE 65200 Tomorrow s quantitation with the TSQ Fortis mass spectrometer: quantitation of phenylephrine hydrochloride for QA/QC laboratories Authors Neloni Wijeratne, Claudia Martins, Mary
More informationThermo Scientific Pesticide Explorer Collection. Start-to-finish. workflows for pesticide analysis
Thermo Scientific Pesticide Explorer Collection Start-to-finish workflows for pesticide analysis Comprehensive Pesticide Analysis Solutions Pesticide Explorer Collection Selection table Lab Profile Routine
More informationPeptide Targeted Quantification By High Resolution Mass Spectrometry A Paradigm Shift? Zhiqi Hao Thermo Fisher Scientific San Jose, CA
Peptide Targeted Quantification By High Resolution Mass Spectrometry A Paradigm Shift? Zhiqi Hao Thermo Fisher Scientific San Jose, CA Proteomics is Turning Quantitative Hmmm.. Which ones are my targets?
More informationHighly Sensitive and Rugged GC/MS/MS Tool
Highly Sensitive and Rugged GC/MS/MS Tool For Pesticide Multiresidue Analysis in Food Samples Agilent 7 Series Triple Quadrupole GC/MS. The world s first MS/MS designed specifically for GC Analysis Introduction
More informationA Rapid Approach to the Confirmation of Drug Metabolites in Preclinical and Clinical Bioanalysis Studies
A Rapid Approach to the Confirmation of Drug Metabolites in Preclinical and Clinical Bioanalysis Studies APPLICATION BENEFITS Regulatory guidelines and recommendations place a greater emphasis on the detection
More informationIDENTIFICATION AND QUANTIFICATION OF TRANSFORMATION PRODUCTS IN THE AQUATIC ENVIRONMENT BY HIGH RESOLUTION MASS SPECTROMETRY
IDETIFICATIO AD QUATIFICATIO OF TRASFORMATIO PRODUCTS I THE AQUATIC EVIROMET BY HIGH RESOLUTIO MASS SPECTROMETRY Juliane Hollender, Rebekka Baumgartner, Kathrin Fenner, Susanne Kern, Philipp Longrée, Heinz
More information