2017 Annual Report for the Molecular Plant Pathogen Detection Lab
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1 2017 Annual Report for the Molecular Plant Pathogen Detection Lab
2 The Molecular Plant Pathogen Detection Lab The Molecular Plant Pathogen Detection (MPPD) Lab utilizes two molecular techniques to identify plant pathogens, conventional polymerase chain reaction (PCR) and real-time PCR. Culturing and morphology and ELISA are also used, as needed. The PCR process allows us to amplify a trace amount of pathogen DNA into a larger and more easily detectable amount of DNA. This technique identifies pathogens much faster than traditional techniques, and identifies pathogens that are difficult to isolate and culture. The main purpose of the lab is to support South Carolina growers in the early detection of plant diseases, especially those that are of USDA-APHIS regulatory concern. The Clemson MPPD lab is accredited under the USDA National Plant Protection Laboratory Accreditation Program (NPPLAP) in molecular diagnostics for HLB (Citrus Greening) and Sudden Oak Death, or Ramorum blight, caused by Phytophthora ramorum. The Lab has taken on other projects as well, such as molecular identification of Africanized honeybees and surface water microbial tracking. The MPPD lab is assisting South Carolina municipalities in meeting EPA water quality standards by doing the water microbial tracking. A real-time PCR method was tested and successfully implemented to differentiate fecal coliforms of animal origin in water samples. In FY 2016, we expanded the water sampling program for counties and municipalities as a service. This project is being done in conjunction with the Clemson Center for Watershed Excellence. These results are not reported in this document. Dr. Curt Colburn, MPPD Lab Director
3 Numbers of Detection Tests Requested Citrus Greening (Huanglongbing) Phytophthora quercina Phytophthora ramorum Boxwood Blight Diaporthe Tobacco Ringspot Nepovirus Africanized Honey Bee
4 Submitter Types by Percent 44 Nonextension commercial Nonextension noncommercial
5 Sample Types by Number of Samples Aquatic Citrus Woody ornamental - Evergreen Vegetables Africanized bee id
6 2017 Molecular Plant Pathogen Detection Lab Survey Detections and Identifications through the Molecular Plant Pathogen Detection Lab in 2017 Confirmed Not detected Suspected Inconclusive AFRICANIZED HONEY BEE TESTING Honey Bee and products (Apis mellifera) European honey bee (Apis mellifera european) BOXWOOD BLIGHT Boxwood (Buxus sp./spp.) Boxwood blight; Leaf and stem blight (Calonectria pseudonaviculata) Common Boxwood (Buxus sempervirens) Boxwood blight; Leaf and stem blight (Calonectria pseudonaviculata) CITRUS SAMPLES Sweet Orange (Citrus sinensis) Citrus greening huanglongbing (Asian) (Candidatus Liberibacter asiaticus) Tangelo (Citrus x tangelo) Citrus greening huanglongbing (Asian) (Candidatus Liberibacter asiaticus) Satsuma; Mandarin; tangerine (Citrus reticulata) Citrus greening huanglongbing (Asian) (Candidatus Liberibacter asiaticus) Key Lime (Citrus aurantifolia) Citrus greening huanglongbing (Asian) (Candidatus Liberibacter asiaticus) Lemon (Citrus limon) Citrus greening huanglongbing (Asian) (Candidatus Liberibacter asiaticus) PHYTOPHTHORA DISEASES ON PLANTS Common Camellia (Camellia japonica) Ramorum blight (Phytophthora ramorum)
7 2017 Molecular Plant Pathogen Detection Lab Survey Confirmed Not detected Suspected Inconclusive PHYTOPHTHORA DISEASES ON PLANTS Northern Red oak (Quercus rubra) Phytophthora crown and root rot (Phytophthora cinnamomi) Phytophthora oak decline; Root rot (Phytophthora quercina) White Oak (Quercus alba) Phytophthora crown and root rot (Phytophthora cinnamomi) Phytophthora oak decline; Root rot (Phytophthora quercina) PHYTOPHTHORA SPECIES IN WATER SOURCES Ponds; Lakes; impounded waters (Aquatic habitat) Phytophthora cinnamomi Phytophthora quercina River; Stream; creek; waterway (Aquatic habitat) Phytophthora quercina Phytophthora cinnamomi Phytophthora ramorum SOYBEAN DISEASES Soybean (Glycine max) Soybean Phomopsis blight (Diaporthe phaseolorum sojae) Tobacco ringspot (TRSV) (Nepovirus Tobacco Ringspot Virus)
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