Biological Control of Bacillus Popilliae against Larvae of Scarabaeid Beetles

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1 Biological Control of Bacillus Popilliae against Larvae of Scarabaeid Beetles KIMURA Masaharu, EHARA Gaku, ITO Kayoko and NISIHASHI Hideji Bacillus specifically infects scarab beetle larvae with milky disease and makes it die. Inefficiency of sporulation in vitro has been a major limiting factor in expanding the use of this bacterium, isolated in the U.S. in the 1930s, though the effect was accepted. Recent years the authors succeeded in the artificial culture of newly isolated Bacillus in Japan and proved the usefulness by field examination. In this paper, the biological control of Bacillus with the unique feature is explained, and the sporulation method is introduced. 1 2 Bp IPM Bp Bp Bp Fig.1 1) Bp 2) Bp Fig.1 The hemolymph of scarab larvae infected with milkey disease. DIC Technical Review No.9 /

2 3) Bp 5,6) Fig.2 The microphotograph of B. spores. Bp m m ) Bp Fig.2 Bp 3 Bp Bp ) Bp Bp ) Bp 1950Bp Bp ) Bp Bp var. mame Bp Bp var. hime Bp Bp var. sakura1998 5) BpBp var. semadara Bp Bp var. Bp Fig.4 Bp Fig.5 Fig.4 Bp spores have the high temperature resistance. 10 DIC Technical Review No.9 / 2003

3 Fig.5 Influence of sunlight to Bp spores. 4 Bp Bp Bp 1 in vivo 2 in vitro a b Fig.6 Preservability of dried Bp spores. 4.1 Bp 1 Popillia japonica ) Rhopaea verreauxi ) 10) Bp Milky Spore Powder hr Fig.63 3 Bp 7) Bp 8) Bp Bp EPA 4.2 Bp Steinkraus Tashiro 11) 12) 13) Fig.7 BpFig.8 DIC Technical Review No.9 /

4 Fig.7 Colonies of Bp var. semadara on solid medium. 19) 20) 21) 1 ml /ml 22) Fig.9 Fig Fig.11 Bp Fig.8 The microphotograph of Bp var. semadara spores formed in colonies on solid medium. 4.3 Bp Bp 14,15) 16) 17) 18) Fig.9 Proceeding of spore productivity. 12 DIC Technical Review No.9 / 2003

5 5.1 9 cm 1 Bp Table 1 Bp diazinon Fig.10 Time course of the spores formation in liquid culture. Table 1 Results of the Field Examination to Strawberries sample conc. pasturage larvae (0 day) survival larvae (43 days) ratio for control spores/m spores/m diazinon control Fig.11 Scanning electron micrograph of Bp var. semadara in liquid culture. Numbers are statistic value. Table 2 Results of the Field Examination to Turf 5 Bp 3) 23) Bp sample conc. pasturage larvae (0 day) Numbers are statistic value. survival larvae (43 days) ratio for control spores/m spores/m diazinon control DIC Technical Review No.9 /

6 5.2 Bp 1 6 m m 2 10 cm Table 2 Bp diazinon 6 Bp 24,25) Bp IPM 1) Dutky, S. R., J. Agric. Res., 61, 57(1940). 2) (1991). 3) Walter E. Fleming, US. Department of Agriculture Technical Bulltin 1383, US. Department of Agricultural Wasihngton D.C(1968). 4) N. Matsuki, S. Asano, H. Bando, T. Iizuka, Appl. Entomol. Zool., 32(4), 583(1997). 5) (1999). 6) (2001). 7) H. D. Burges, Lundholm, B. & Stackerud, M.(eds) Enviromental Protection and Boilogical Forms of Control Pest Organisms Ecol. Bull. (Stockholm), 31, 81(1980). 8) A. M. Heimpel, G. G. Hrubant, Environmental Entomology, 2(5), 793(1973). 9) R. J. Milner, J. Invertebr. Pathol., 23, 289(1974). 10) R. V. Vyas, D. N. Yadav and R. J. Patel, GAU Res. J., 17(1), 30(1991). 11) K. H. Steinkraus, H. Tashiro, Science, 121, 873(1955). 12) R. A. Rhodes, M. S. Roth, and G. R. Hrubant, Can. J. Microbiol., 11, 779(1965). 13) J. W. Foster, W. A. Hardwick, and Beverly Guirard, J. Bacterol., 59, 463(1950). 14) W. C. Haynes, Lenora J. Rhodes, J. Bacterol., 91, 2270(1966). 15) William C. Haynes, Lenora J. Weih, Clarence Crowell, Can. J. Microbiol., 18, 515(1972). 16) William C. Haynes, Lenora J. Weih, J. Invertebr. Pathol., 19, 125(1972). 17) Ralph N. Costilow, Charles J. Sylvester, Appl. Microbiol., 14, 161(1966). 18) William C. Haynes, Clarence D. Crowell, J. Invertebr. Pathol., 22, 377(1973) 19) E. S. Sharpe, Lee A. Bulla, JR., Appl. Environ. Microbiol., 35(3), 601(1978). 20) Reuter Laboratories, Inc., US Pat (1989). 21) D. P. Stahly, M. G. Klein, J. Invertebr. Pathol., 60, 283(1992). 22) (2002). 23) Feng Xiang-xing, Shing Shing-chiu, Yang Ming-hua, Acta Entomological Sinica, 25(2), 156(1982). 24) J. Zhang, T. Charles Hodgman, et. al., J. Bacterol., 179(13), 4336(1997). 25) J. Zhang, H. U. Schairer, et. al., Nucleic Acids Research, 26(5), 1288(1998). KIMURA Masaharu ITO Kayoko EHARA Gaku NISIHASHI Hideji 14 DIC Technical Review No.9 / 2003