Supplementary Figure 1 Characterization of wild type (WT) and abci8 mutant in the paddy field.

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1 Supplementary Figure 1 Characterization of wild type (WT) and abci8 mutant in the paddy field. A, Phenotypes of 30-day old wild-type (WT) and abci8 mutant plants grown in a paddy field under normal sunny days (A) followed by about two weeks of cloudy/rainy conditions (B). They were transplanted to pots prior to be photographed. Phenotype of the abci8 mutant at tillering (C) and heading stage (D) after grown continuous rainy days for more than one month in the paddy field. E, Phenotype of WT and abci8 mutant at seed filling stage. F, Characterization of plant height, and tiller number in WT and abci8 mutant. Values shown were means±sd (n=30). Statistically significant differences were indicated by double asterisks (**P<0.01). Bar = 10 cm.

2 Supplementary Figure 2 Characterization of wild type (WT) and abci8 under different intensities of white light. A, Seedling phenotypes of WT and abci8 mutant under different intensities light conditions. NL, Normal white light (~100 μmol m -2 s -1 ); ML, Middle white light (14 μmol m -2 s -1 ); LL, Low white light (1.5μmol m -2 s -1 ); VL, Very low white light (0.2 μmol m -2 s -1 ). Photos were taken after treatment of different intensities light for 12 days. Bar=2 cm. B, Analysis of different pigments of WT and abci8 mutant seedlings grown under different intensities light conditions. Values were means±sd of three biological replicates. Chla, chlorophyll a; Chlb, chlorophyll b; Car, carotenoid.

3 Supplementary Figure 3 Identification of T-DNA insertion mutation in the OsABCI8 gene. A, Diagrammatic illustration of the rice ABCI8 gene showing the site of the T-DNA insertion. B, PCR-based analysis on the genomic DNAs of the wild-type control, the T-DNA insertion heterozygous (H) and homozygous (M) mutant. C, RT-PCR analysis of gene expression of ABCI8 in the wild-type plants (WT) and the T-DNA insertion mutant. ACTIN was used as a control. To perform RT-PCR analysis, leaves from WT and albino plants were collected from 35-day-old seedlings that were grown in a paddy field under 12 sunny days followed by 23 days of cloudy/rainy conditions. D, Thirty-five-old wild-type and mutant (M) seedlings are shown.

4 Supplementary Figure 4 Elemental analysis of Fe in the wild-type (WT) and mutant (M) plants with a T-DNA insertion mutation in the OsABCI8 gene at the albino stage. The plants were grown in a paddy field for 12 sunny days followed by 23 day of cloudy/rainy conditions. Data are means ± SD of three biological replicates. Significant difference from the corresponding wild-type value, based on the Student s t-test, are marked by ** (P < 0.01).

5 Supplementary Figure 5 Fe content of 4-week old WT and abci8 seedlings grown under white light conditions. The plants were grown in soil from a rice paddy with a 13/11h light/darkness photoperiod at a photon flux density of 150 µmol m 2 s 1 and constant temperature of 28 C in a growth chamber, after incubating seeds in darkness for 48 h at 26 C to ensure synchronized germination. Data are means ± SD of three biological replicates.

6 Supplementary Table 1 List of primers used in this study Primers Sequence (5 3 ) Experiments ID8-11F GCTTTGCTGTCTCCTTGGTC Map-based cloning ID8-11R TCGTGTGTGTAGCAGGATCA Map-based cloning RM26730F AATTCCTCCGGGTTCCCAATGC Map-based cloning RM26730R TGATCCCACTAACGACCAAGGTAAGG Map-based cloning RM26739F AAACAGTAGTTGGGTTGACTGG Map-based cloning RM26739R GGACGAGAGGAGTGTTAATTCG Map-based cloning IDM-2F AATGGCAAAGATGAACACTGG Map-based cloning IDM-2R TCCAAGACATTTGCTTTTACCA Map-based cloning IDM-4F TATCGAGGGTGCACCATTTT Map-based cloning IDM-4R CACTAGATCAAAGAGGCAGCG Map-based cloning ID7-22F TCCATTAAATTTCCAACCTCCT Map-based cloning ID7-22R AAAAACTACTCCCTCCGTTTCAT Map-based cloning ABC-gF GGTACCTGGCCTTACATTGCAGATCA Functional complement assay ABC-gR GGATCCGACCACAAGGTGTACCTATTTC Functional complement assay ABC-MF CCTGCGGAAATCTAGGGTCCT RT-PCR ABC-MR CACGATACCAACTCTTTGAGCAG RT-PCR I8RT80F TGCGGAAATCTAGGGTCCTC qrt-pcr I8RT351R GGTTCCTGGAGGTCGATAGC qrt-pcr I8RTR TCAGTGTTGTTTTGCTCTGC qrt-pcr I8AF GAAGCTTTATCTGTGAGCA qrt-pcr I8BF AGAAGCTTTATCTGTGTAAG qrt-pcr I8BR CTGAAATTCTCAAACTGCAC qrt-pcr ABC-EGFPF ACTAGTTCTAGAATGCAGGTGGTGGGGACGGC Subcellular localization ABC-EGFPR CCATGGGGATCCCACAGATAAAGCTTCTTCCT Subcellular localization ABC-GEXANF GGATCCCACTAGTATGCAGGTGGTGGGGACGGC Subcellular localization ABC-GEXANR GAATTCTCATCTAGAATTATCTCTCCATTGAAG Subcellular localization ABC-TL GCACTTCTTTGTGGGCATCG Identification of T-DNA insertion ABC-TR CTACCTGGCAGACAGGCATC Identification of T-DNA insertion NTLB5 AATCCAGATCCCCCGAATTA Identification of T-DNA insertion ACTINF AGCAACTGGGATGATATGGA RT-PCR endogenous control ACTINR CAGGGCGATGTAGGAAAGC RT-PCR endogenous control

7 Supplementary Table 2 Elemental analysis (mg. kg -1 ) Elements WT/abci8 Value 1 Value 2 Value 3 Average SD Mn WT abci Ca WT abci Mg WT abci Zn WT abci Fe WT abci Ni WT abci