Introduction to Chemical Research (CHEM 294) Introduction to Chemical Research (CHEM 294)

Transcription

1 Introduction to Chemical Research (CHEM 294) Mondays: Lecture 1-2 pm CRN: Lab 2:15-5:15 pm Mentoring 6. Do Preliminary Experiments 5. Plan the Experiments 1.Pose a Research Idea Proposal to submit for funding to continue the project 2. Review Primary Literature 3. Brainstorm Project Ideas Proposal 4. Pose a Testable Hypothesis Introduction to Chemical Research (CHEM 294) Mondays: CRN: Lecture 1-2 pm Lab 2:15-5:15 pm CHEM 294 Introduction to Chemical Research CHEM 488 Chemical Research CHEM 694 Mentoring Chemical Research Target audience: Sophomores Target audience: Junior/Senior Target audience: Graduate students 1

2 Analy,cal Instrumental Laboratory CHEM 314W Introduc,ons: 1. Syllabus and Course Structure 2. Course website 3. Course projects 4. Overview of Techniques 5. Paper Format Ac,vity Dr. Sarah Hayes Asst. Professor 2

3 I m Dr. Hayes Research: Geochemistry of toxic metal(loid)s Geochemistry of Mineral weathering Mobility and transport Bioaccessibility Human and ecosystem exposure and Health Textbooks: Skoog and Holler 6 th ed Harris 8 th ed On reserve at the Rasmussen Library for 2 hr use 3

4 Course Evalua,on Consumer Product Characteriza,on Project Colors & Calories Electron Microscopy ID Plas,cs CYOA Elemental Analysis Consumer Product Separa,ons 4

5 Project Defini,on A 3-5 pg report designed facilitate learning about your product and develop a plan for addressing your ques,ons. 1. IntroducGon- Tell me about your product. 2. Product InformaGon- Research your consumer product. 3. Ingredients- Learn more about your product ingredients. 4. The Plan (Reports 1-5)- How will you address each quesgon relevant to your product. 5. Choose Your Own Adventure (Report 6)- Propose two ques,ons of your choosing. 6. References 7. Appendices Experiments Progression Standard Addi,on Experiment 1: Elemental Analysis Experiment 2: PlasGcs Experiment 3: Colors Experiment 4: SEM Experiment 5: CYOA Experiment 6: SeparaGons Throughput of an op,cal instrument 5

6 Experimental Progression 1. Procedure 2. Experiment 3. Annotated Figures 4. Report 5. Peer Review 6. Revision The Schedule 6

7 Due Dates CHEM 314- h\ps://instrumentalanalysis.community.uaf.edu 7

8 How will we address each ques,on? Which technique answers which Q? Colors & Calories Electron Microscopy ID Plas,cs CYOA Elemental Analysis Consumer Product Separa,ons 8

9 Techniques for elemental analysis Handheld XRF Dissolve metal Major element Trace metals Iron Not detectable? Aluminum Other? HNO 3 Flame AA ICP-MS HCl SEM, XRF ICP-MS??? Which technique answers which Q? Colors & Calories Electron Microscopy ID Plas,cs CYOA Elemental Analysis Consumer Product Separa,ons 9

10 Plas,cs Iden,fica,on Probes vibra,ons of func,onal groups within sample Flame test FT-IR Step 1: prepara,on of plas,cs Plas,cs Iden,fica,on Step 2: FT-IR analysis Video online Step 3: Spectral interpreta,on 10

11 Plas,cs Iden,fica,on by other methods h\p://ides blogspot.com/2011/03/plas,cs-iden,fica,on-charts.html Which technique answers which Q? Colors & Calories Electron Microscopy ID Plas,cs CYOA Elemental Analysis Consumer Product Separa,ons 11

12 Colors- Molecular Spectroscopy UV-Vis Fluorescence FT-IR Calorimeter- energy per mass h\ps://people.chem.umass.edu/botch/chem122s08/chapters/ch6/calorimbomb.jpg 12

13 Which technique answers which Q? Colors & Calories Electron Microscopy ID Plas,cs CYOA Elemental Analysis Consumer Product Separa,ons Electron Microscopy 13

14 Step 1: prepara,on of samples Step 2: carbon or gold coat samples Step 3: Electron microscopy Electron Microscopy Which technique answers which Q? Colors & Calories Electron Microscopy ID Plas,cs CYOA Elemental Analysis Consumer Product Separa,ons 14

15 Choose your own adventure (CYOA) You must propose three ideas in the project proposal Poten,al ideas: Iden,fica,on/quan,fica,on or molecules using NMR X-ray diffrac,on (XRD) to iden,fy mineral species (or some organics) X-ray fluorescence (XRF) to quan,fy elements Addi,onal Separa,ons Addi,onal color examina,on Which technique answers which Q? Colors & Calories Electron Microscopy ID Plas,cs CYOA Elemental Analysis Consumer Product Separa,ons 15

16 High Performance Liquid Chromatography (HPLC) Step 1: samples adjusted for concentra,on Step 2: samples separated in column Step 3: samples detected using UV-Vis or EDLS High Performance Liquid Chromatography (HPLC) Iden,fied on the basis of: Reten,on,me Light sca\ering UV-Vis absorp,on Mass Spectrometry 16

17 Gas Chromatography- Mass Spectrometry (GC-MS) Step 1: sample injec,on: liquid or headspace Step 2: samples separated in column Step 3: totals molecules counted Step 4: molecules fragmented Step 5: ion fragments detected Gas Chromatography- Mass Spectrometry (GC-MS) Headspace or direct liquid injec,on Iden,fied on the basis of: Reten,on,me Mass spectrum 17

18 Project-based course structure Colors & Calories Electron Microscopy ID Plas,cs CYOA Elemental Analysis Consumer Product Separa,ons Paper format exercise Structure Effec,ve wri,ng Content What goes where in a paper? 18