trans,trans-muconic ACID IN URINE BY UV - CODE Z23010
|
|
- Leonard Bell
- 6 years ago
- Views:
Transcription
1 trans,trans-muconic ACID IN URINE BY UV - CODE Z BIOCHEMISTRY The measuring of trans,trans-muconic Acid is suggested as a biological and reliable marker for monitoring workers exposed to benzene. Benzene is an industrial chemical product which often appears in mineral oils following combustion processes. For this reason there is a high risk of environmental pollution. Its levels in the air can easily increase due to emissions from coal and oil burning, benzene leak and storage operations, exhaust fumes from cars, evaporation from gasoline service stations, and use of industrial solvents. Smoking is another source of benzene in air since tobacco contains high levels of benzene. Acute exposure to benzene can cause drowsiness, dizziness, rapid heart rate, headaches, tremors, confusion, unconsciousness and death. People who breathe benzene for long periods may have different harmful effects. In fact, it is haematoxic and has been identified as a carcinogen, can bring to anaemia, excessive bleeding and it can be harmful to the immune system and the reproductive organs. Regarding cancerogenesis, according to the American Department of Health and Human Services ( DHHS ) benzene is a human carcinogen. Long-term exposure to relatively high levels of benzene in the air can cause leukemia. For all these reasons monitoring and control of workers exposed to benzene is very important Benzene is primarily metabolised by a cythocrome P- ( CYP- ) to phenolic compounds. The range of metabolic conversion of benzene to t,t-ma is about % and dependent on the benzene exposure level. Once oxidized, the metabolites play a substantial role in benzene s toxicity by inducing either DNA alkylation or oxidative stress that lead to the above mentioned systemic disorders. EUREKA srl LAB DIVISION VAT N info@eurekaone.com Head Quarter: Via Enrico Fermi Chiaravalle (AN) ITALY Tel Fax This product fulfills all the requirements of Directive 9/9/EC on in vitro diagnostic medical devices (IVD). The declaration of conformity is available upon request. Rev. N T,t-Muconic Acid in urine by UV December
2 TECHNICAL FEATURES Principle of the Method: trans,trans-muconic Acid is diluted with a suitable diluent and after purification in a clean up column it is injected into the HPLC System. Recovery: > 9% Sensitivity: < µg /l CV% :,% Exposure Biological Index E.B.I. : Acceptable values in urine of not exposed subjects: Metabolic Interferences : t,t-ma µg / l This value is due to the Exposure Average of ppm of Benzene in the air <, mg/g creatinin (not smokers) Ref.: G Ital Med Lav Erg ;: Suppl Sorbic Acid : about, % of Sorbic Acid got with diet is converted in t,t-ma Components of the kit : All the components are ready-to-use and stable years at room temperature ( C ). The Urine Calibrator liophyl. is stable months at - C. Reagent A Dilution Solution, x ml Reagent B Conditioning Solution N, x ml Reagent C Conditioning Solution N, x ml Reagent D Conditioning Solution N, x ml Reagent E Wash Solution N, x ml Reagent F Wash Solution N, x ml Reagent G Eluting Solution, x ml Reagent H Test Solution, x ml Store at - C Reagent I Internal Standard Solution, x ml Store at - C Reagent U Urine Calibrator lyoph., x ml See Warnings Reagent M Mobile Phase, x ml Clean up Columns, pcs Minimum Instrumental equipment required: Isocratic HPLC System with loop of µl Spectrofotometric Detector UV/VIS λ= nm Chromatograms Recorder Optional Equipment: Autosampler Operational Computer Urine Collection Procedure: After work shift, collect ml of urine in a suitable test tube. Delayed analyses require sample freezing at C or less. Stable over weeks.
3 PREANALYTICAL PROCEDURE Test Solution at µg/l Preparation. Dispense in a tube : 9 µl of Water HPLC grade µl of Reagent H Test Solution µl of Reagent I Internal Standard Solution INJECTION : Vortex for seconds Inject µl of this solution in the chromatographic system. Verify that the Test Solution has retention time similar to fig.. If the Test is all right you can start with the analytical procedure; if not, check the functionality of the analytical system. Important : Don t use this solution to calibrate! ANALYTICAL PROCEDURE STEP : Calibrator, Samples and Controls preparation As soon as the urine samples are ready for processing check if the ph is >. Dispense in a tube : Blank Calibrator Sample Water HPLC grade µl Reagent U Urine Calibrator µl Sample µl Reagent A Diluting Solution µl µl µl Reagent I Internal Standard Sol. µl µl µl Vortex for seconds STEP : Prepare Clean up columns per each Sample, Calibrator and Controls. Condition clean up columns by fluxing, ml of Reagent B-Conditioning Sol. and don t let the columns be dry Condition clean up columns by fluxing, ml of Reagent C- Conditioning Sol. and don t let the columns be dry Condition clean up columns by fluxing, ml of Reagent D- Conditioning Sol. and don t let the columns be dry STEP : Sample loading Dispense, ml of sample, previously prepared, into clean up columns Discharge the percolated liquid, if needed a vacuum system could be used for percolation, at speed of ml/min ( drop by drop ). STEP : Washing Wash with ml of Reagent E - Wash Solution discharge the percolated liquid Wash with ml of Reagent F - Wash Solution discharge the percolated liquid Rev. N T,t-Muconic Acid in urine by UV December
4 STEP : Extraction Extract with ml of Reagent G - Eluting Solution Let dry with a light flux of air! Vortex for seconds N.B.: at this step, the sample is stable days at - C INJECTION : Inject µl of the eluate into the chromatographic system. Rev. N T,t-Muconic Acid in urine by UV December
5 REAGENT H : TEST SOLUTION trans,trans-muconic ACID IN URINE Warnings trans,trans-muconic ACID. µg / l REAGENT U : URINE CALIBRATOR LYOPHIL.- Lot N trans,trans-muconic ACID Use and Reconstitution: Urine Calibrators are used for calibration of the HPLC system. This lyophilised calibrator has to be prepared like a patient sample. Add exactly. ml of HPLC water to the vial and mix for min. When all material is dissolved, the solution is ready to use. Stability: months if stored at - C. After reconstitution the stability of the analytes is at least hours when stored at + C, at least days at + C and at least month at - C. Don t use after expiry date. Mix always before use. Packaging: x ml Warning: The calibrator derives from human urine, so it could be potentially infected. It must be handled with care., µg / l PARAMETERS FOR THE SPECTROFOTOMETRIC DETECTOR λ GAIN INTEGRATION TIME nm, AUFS sec HPLC COLUMN PROTECTION To save the analytical column Reverse Phase GENESIS, x mm, µ, the use of Metasaver Precolumn Filter. um ( x pcs.) cod. ZA is obligatory. HPLC COLUMN CONDITIONING COLUMN CLEANING Install a new analytical C Reversed-Phase column (GENESIS mm x, mm, µ). Disconnect the detector and filter ml of H O: Methanol ( : v/v ) solution and subsequently ml of H O per HPLC, set flow at, ml / min. Don t recycle the washing solutions. Filter the mobile phase with a vacuum system and a suitable filter of, µ. Condition the column with the mobile phase at a flow of, ml / min. and discharge the first ml. Condition further on the column for min. also at recycling phase. Finally inject the Chemical Standard and verify the quality of the HPLC run. It is NOT possible to make analysis at recycling phase. If room temperature is > C store the Mobile Phase at - C between an analytical session and another. Disconnect ESA detector. Flux ml of H O for min and discharge. Flux a solution made of H O : Methanol ( : v/v) for min and discharge. When you re-use the column, flux ml of H O before a new conditioning with the Mobile Phase. INJECTION NEEDLE WASHING HPLC PARAMETERS Wash with a solution of Water / Ethanol ( : v/v). LOOP µl Recommended Flow rate, ml / min Pressure About bar INTEGRATOR HP 9 / 9 / 9 PARAMETERS ATTENUATION OPERATIONAL COMPUTER PARAMETERS IN CONFORMITY WITH THE SPECIFICATION OF OPERATIONAL COMPUTER SOFTWARE
6 ACCESSORIES AND CONSUMABLES CODE DESCRIPTION PACKAGING Z Urine Calibrator lyophil. for t,t-muconic Acid x ml Z Urine multiparametric Control lyophil. for toxic organic compounds, Level x ml Z Urine multiparametric Control lyophil. for toxic organic compounds, Level x ml Z9 Urine multiparametric Control lyophil. for toxic organic compounds, Levels, x x ml ZFM9E Genesis C ( x,mm - u) Analytical Column Pk ZA Metasaver Precolumn Filter. um x PK S9U Standard glass vials of ml with screw cap x PK
7 t,t-muconic ACID (Reference Chromatograms) mv test fmvertua-isop.data mv.data TTM IS 9 - TTM IS - 9 RT[min] RT[min] 9 Fig. Test Solution Fig. Urine Calibrator lyophil. R.T.. t,t-muconic Acid R.T.. t,t-muconic Acid µg/l R.T.. Internal Standard R.T..9 Internal Standard
8 t,t-muconic ACID (Reference Chromatograms) mv 9.DATA mv camp.data IS IS TTM TTM - 9 RT[min] 9 9 RT[min] 9 Fig. Urine Control lyophil. Level Fig. Urine Sample R.T.. t,t-muconic Acid µg/l R.T.. t,t-muconic Acid, µg/l R.T.. Internal Standard R.T.. Internal Standard
HIPPURIC ACID and o-m-p- METHYLHIPPURIC ACIDS IN URINE BY UV CODE Z06010
HIPPURIC ACID and o-m-p- METHYLHIPPURIC ACIDS IN URINE BY UV CODE Z06010 Biochemistry The determination of Hippuric Acid ( HPA ) in urine is important to determine the risk due to the exposition to Toluene.
More informationACETONE IN URINE BY UV CODE Z42010
ACETONE IN URINE BY UV CODE Z42010 BIOCHEMISTRY Acetone is a colorless, mobile, flammable liquid. It is readly soluble in water, ethanol, ether etc., and itself serves as an important solvent. Acetone
More information25-OH VITAMIN D 3 IN PLASMA BY UV FAST CODE Z19610
25-OH VITAMIN D 3 IN PLASMA BY UV FAST CODE Z19610 BIOCHEMISTRY Quantification of 25-hydroxyvitamin D 3 (25-hydroxycholecalciferol) in serum is the best established indicator of vitamin D status. Vitamin
More information25-OH VITAMIN D 3 AND 25-OH VITAMIN D 2 IN PLASMA BY UV / VIS FAST CODE Z19710
25-OH VITAMIN D 3 AND 25-OH VITAMIN D 2 IN PLASMA BY UV / VIS FAST CODE Z19710 BIOCHEMISTRY Quantification of 25-hydroxyvitamin D 3 (25-hydroxycholecalciferol) in serum is the best established indicator
More informationHIPPURIC ACID, o-m-p-methylhippuric ACIDS and CREATININE IN URINE by UV CODE Z07510
HIPPURIC ACID, o-m-p-methylhippuric ACIDS and CREATININE IN URINE by UV CODE Z07510 Biochemistry The determination of Hippuric Acid ( HPA ) in urine is important to determine the risk due to the exposition
More informationFORMALDEHYDE IN URINE by UV Code Z65010
FORMALDEHYDE IN URINE by UV Code Z65010 BIOCHEMISTRY The dose of urinary Formaldehyde is made after exposure to Formaldehyde and Methanol. The Formaldehyde is a pollutant so-called ubiquist, because it
More informationHYDROXYPROLINE IN URINE by UV/VIS Code Z01010
HYDROXYPROLINE IN URINE by UV/VIS Code Z Chemical characteristics Hydroxyproline or (S,R)--Hydroxy-L-pyrrolidine--carboxylic acid, is an aminoacid with an hydroxyl group on the pyrrolidine ring, in position.
More informationMETHANOL (METHYLIC ALCOOL) IN URINE BY UV CODE Z05610
METHANOL (METHYLIC ALCOOL) IN URINE BY UV CODE Z05610 METABOLISM Methanol is an aliphatic alcohol with PM=32,0. Methanol, also known as methyl alcohol, carbinol, wood alcohol, wood naphtha or wood spirits,
More informationGLUTATHIONE IN WHOLE BLOOD BY FLUORIMETRY CODE Z30010
GLUTATHIONE IN WHOLE BLOOD BY FLUORIMETRY CODE Z30010 INTRODUCTION Glutathione (GSH) is a tripeptide. It contains an unusual peptide linkage between the amine group of cysteine and the carboxyl group of
More information3-METHYL INDOLE (Skatole) IN URINE BY FLUORIMETRY FAST - Code Z60010
3-METHYL INDOLE (Skatole) IN URINE BY FLUORIMETRY FAST - Code Z60010 BIOCHEMISTRY 3-methyl indole or skatole (P.M. 131.172) is an organic compound which presents, as structural unit, indolic group. It
More informationHYDROXYPROLINE IN URINE by Fluorimetry FAST Code Z12610
HYDROXYPROLINE IN URINE by Fluorimetry FAST Code Z12610 Chemical characteristics Hydroxyproline or (2S,4R)-4-Hydroxy-L-pyrrolidine-2-carboxylic acid, is an aminoacid with an hydroxyl group on the pyrrolidine
More informationMETHADONE and EDDP in urine by GC-MS Code GC Method of Confirmation by GC-MS
METHADONE and in urine by GC-MS Code GC48010 Method of Confirmation by GC-MS INTRODUCTION is a synthetic opioid used as a drug of choice in the detoxification of heroin (and / or opiates). is metabolized
More informationNORBUPRENORPHINE (Buprenorphine s Metabolite ) BUPRENORPHINE in urine by GC/MS Code GC Method of Confirmation by GC-MS
NORBUPRENORPHINE (Buprenorphine s Metabolite ) BUPRENORPHINE in urine by GC/MS Code GC44010 Method of Confirmation by GC-MS INTRODUCTION Buprenorphine is an analgesic with a long-time action, 25 to 40
More informationBUTYL ALCOHOL in urine by GC-FID Code GC05510
BUTYL ALCOHOL in urine by GC-FID Code GC00 BIOCHEMISTRY Butanol (or n-butyl alcohol) is an alcohol which at room temperature is presented as a colorless liquid smelling of alcohol. It is an inflammatory
More informationFORMIC ACID in URINE by GC/MS-headspace - Code GC74010
FORMIC ACID in URINE by GC/MS-headspace - Code GC74010 BIOCHEMISTRY The Methanol is used in the production of cellulose, synthetic resins, formaldehyde, acetic acid and fuel. The route of exposure is more
More informationTETRACHLOROETHYLENE IN URINE BY GC/MS Headspace Code GC15010
TETRACHLOROETHYLENE IN URINE BY GC/MS Headspace Code GC15010 BIOCHEMISTRY The Tetrachlorethylene or perchlorethylene, is an organic halide. Its structure is similar to that of a molecule of ethene which
More informationACETONE IN URINE BY GC/MS Headspace Code GC10110
ACETONE IN URINE BY GC/MS Headspace Code GC10110 BIOCHEMISTRY The acetone or dimethyl ketone is a product which falls into the biochemical physiological small amounts are formed as a product of the metabolism
More informationTRICHLOROETHYLENE IN URINE BY GC/MS Headspace Code GC16110
TRICHLOROETHYLENE IN URINE BY GC/MS Headspace Code GC16110 BIOCHEMISTRY Trichlorethylene, marketed under the name of thinner, is used as a degreaser of metals, preparation of paints and cleaning of fabrics.
More informationMETHYLISOBUTYLKETONE (4-methyl-2 pentanone) IN URINE BY GC/MS Headspace Code GC10510
METHYLISOBUTYLKETONE (4-methyl-2 pentanone) IN URINE BY GC/MS Headspace Code GC10510 BIOCHEMISTRY The methyl isobutyl ketone is a colorless and flammable liquid that is used as organic solvent in the laboratory
More informationMETHYLETHYLKETONE (M.E.K.) IN URINE BY GC/MS in head space Code GC10010
METHYLETHYLKETONE (M.E.K.) IN URINE BY GC/MS in head space Code GC10010 BIOCHEMISTRY The MEK is presented as a colorless liquid odor similar to that of acetone, is volatile and potentially explosive. The
More informationDETERMINATION OF FREE CATECHOLAMINES WITH RESIN IN URINE - CODE Z10110 (Derivatization kit for Fluorimetry detection)
DETERMINATION OF FREE CATECLAMINES WITH RESIN IN URINE - CODE Z10110 (Derivatization kit for Fluorimetry detection) BIOCHEMISTRY Catecholamines are dihydroxyl organic compounds characterized by a phenolic
More informationFREE CATECHOLAMINES IN URINE BY FLUORIMETRY FAST CODE Z10610
FREE CATECHOLAMINES IN URINE BY FLUORIMETRY FAST CODE Z BIOCHEMISTRY Catecholamines are dihydroxyl organic compounds characterized by a phenolic ring (fig.). Epinephrine (E), Norepinephrine (NE), and Dopamine
More informationTOTAL DIVIDED METANEPHRINES IN URINE - FAST CODE Z12710 ( by FLUORIMETRY )
TOTAL DIVIDED METANEPHRINES IN URINE - FAST CODE Z12710 ( by FLUORIMETRY ) BIOCHEMISTRY Catecholamines are dihydroxyl organic compounds characterized by a phenolic ring (fig.1). Epinephrine (E), Norepinephrine
More informationVMA, HVA, 5-HIAA Urine LC-MS/MS Analysis Kit User Manual
Page 1 / 11 VMA, HVA, 5-HIAA Urine LC-MS/MS Analysis Kit User Manual ZV-3030-0200-20 200 2-8 C Page 2 / 11 Table of Contents 1. INTENDED USE... 3 2. SUMMARY AND EXPLANATION... 3 3. TEST PRINCIPLE... 3
More informationCatecholamines Urine LC-MS/MS Analysis Kit User Manual
Page 1 / 13 Catecholamines Urine LC-MS/MS Analysis Kit User Manual ZV-3031-0200-20 200 Page 2 / 13 Table of Contents 1. INTENDED USE... 3 2. SUMMARY AND EXPLANATION... 3 3. TEST PRINCIPLE... 3 4. WARNING
More informationZinc-Protoporphyrin/ Protoporphyrin HPLC Kit
Distribuito in ITALIA da Li StarFish S.r.l. Via Cavour, 35 20063 Cernusco S/N (MI) telefono 02-92150794 fax 02-92157285 info@listarfish.it www.listarfish.it Manual Zinc-Protoporphyrin/ Protoporphyrin HPLC
More informationTechnical Procedure for Concentration Determination of Methamphetamine in Liquids via HPLC
Technical Procedure for Concentration Determination of 1.0 Purpose This procedure specifies the required elements for the preparation and use of the Agilent 1100/1200 series High Performance Liquid Chromatograph
More informationMethod of confirmation by GC-MS
9 - TETRAHYDROCANNABINOL in hair by GC-MS Code GC53010 Method of confirmation by GC-MS INTRODUCTION In the early 80 's, began a series of studies on the possibility of determining substances of abuse in
More informationPsychoactive Drugs Urine LC-MS/MS Analysis Kit User Manual
Page 1 / 18 Psychoactive Drugs Urine LC-MS/MS Analysis Kit User Manual ZV-2001-0200-20 200 2-8 C Page 2 / 18 Table of Contents 1. INTENDED USE... 3 2. SUMMARY AND EXPLANATION... 3 3. TEST PRINCIPLE...
More informationBritish American Tobacco Group Research & Development. Method - Determination of ammonia in mainstream smoke
British American Tobacco Group Research & Development Method - Determination of ammonia in mainstream smoke 1 SCOPE OF APPLICATION The method is applicable to quantitative determination of the yields of
More informationMicro Volume QuEChERS kit
225-37872 Sep. 2018 Small Capacity Pretreatment Kit Micro Volume QuEChERS kit Instruction Manual Read this manual thoroughly before you use the product. Keep this manual for future reference. This page
More informationDetection limit: grain, feed 500 ppb; milk 50 ppb; cream, cheese 5 ppb
Product information Background Deoxynivalenol (DON) Deoxynivalenol, called vomitoxin, is a toxic metabolite mainly produced by Fusarium graminearum. It is mainly found in wheat, barley, corn and feed.
More informationQuantitative Analysis of EtG and EtS in Urine Using FASt ETG and LC-MS/MS
Quantitative Analysis of EtG and EtS in Urine Using FASt ETG and LC-MS/MS UCT Part Numbers: CSFASETG203 - CLEAN SCREEN FASt ETG, 200mg / 3mL tube SLETG100ID21-3UM - Selectra ETG HPLC column, 100 x 2.1
More informationQuantitative Analysis of EtG and EtS in Urine Using FASt ETG and LC-MS/MS
Quantitative Analysis of EtG and EtS in Urine Using FASt ETG and LC-MS/MS UCT Part Numbers: CSFASETG203 - CLEAN SCREEN FASt ETG, 200mg / 3mL tube SLETG100ID21-3UM - Selectra ETG HPLC column, 100 x 2.1
More informationAnalysis of BTEX in Natural Water with SPME
Analysis of BTEX in Natural Water with SPME Application Note Environmental Author Giordano Vassalli Sezione Protezione Aria Introduction Benzene, toluene, ethylbenzene and xylene (BTEX) isomers are monocyclic
More informationTECHNICAL BRODIFACOUM
BRODIFACOUM Full specification: Approved 10 December 1999 1. Specification 1.1 Description The material shall consist of brodifacoum together with related manufacturing impurities and shall be in the form
More informationBritish American Tobacco Group Research & Development. Method - Determination of phenols in mainstream cigarette smoke
British American Tobacco Group Research & Development Method - Determination of phenols in mainstream cigarette smoke 1 SCOPE OF APPLICATION The method is applicable to the quantitative determination of
More informationanti-httg IgA ELISA For the in vitro determination of anti-human-tissue- Transglutaminase IgA antibodies in serum and plasma Valid from
Li StarFish S.r.l. Via Cavour, 35-20063 Cernusco S/N (MI), Italy Tel. +39-02-92150794 - Fax. +39-02-92157285 info@listarfish.it -www.listarfish.it l ELISA For the in vitro determination of anti-human-tissue-
More informationPlease use only the valid version of the package insert provided with the kit. This kit is intended for Research Use Only.
Please use only the valid version of the package insert provided with the kit This kit is intended for Research Use Only. Not for use in diagnostic procedures. INTENDED USE The Vitamin Niacin test is a
More informationDetermination of underivatized aflatoxins B2, B1, G2, and G1 in ground hazelnuts by immunoaffinity solid-phase extraction with HPLC-FLD detection
APPLICATION NOTE 72686 Determination of underivatized aflatoxins,, G2, and in ground hazelnuts by immunoaffinity solid-phase extraction with HPLC-FLD detection Authors Sylvia Grosse, Mauro De Pra, Frank
More informationß 2. -Microglobulin ELISA. For the in vitro determination of ß 2. -microglobulin in plasma, serum and urine K Manual. Valid from
Distribuito in ITALIA da Li StarFish S.r.l. Via Cavour, 35 20063 Cernusco S/N (MI) telefono 02-92150794 fax 02-92157285 info@listarfish.it www.listarfish.it Manual ELISA For the in vitro determination
More informationMaximizing Triple Quadrupole Mass Spectrometry Productivity with the Agilent StreamSelect LC/MS System
Maximizing Triple Quadrupole Mass Spectrometry Productivity with the Agilent StreamSelect LC/MS System Application Note Authors Kevin McCann, Sameer Nene, Doug McIntyre, Edmond Neo, Dennis Nagtalon, and
More informationFormaldehyde Fluorescent Detection Kit
K-ASSAY KAMIYA BIOMEDICAL COMPANY KAMIYA BIOMEDICAL COMPANY Formaldehyde Fluorescent Detection Kit For detection in human urine and tissue culture media Cat. No. KT-723 For Research Use Only. Not for Use
More informationANALYTICAL METHOD DETERMINATION OF VOLATILE ALDEHYDES IN AMBIENT AIR Page 1 of 11 Air sampling and analysis
DETERMINATION OF VOLATILE ALDEHYDES IN AMBIENT AIR Page 1 of 11 Replaces: Dated: Author: Date: AM-No.: New New Nils Arne Jentoft 18.06.2014 0 CHANGES This procedure is new. 1 SCOPE This document describes
More informationMETHOTREXATE mg/m 3 (arbitrary). There is no OSHA PEL or AGGIH TLV for
METHOTREXATE Matrix: Air Control no.: T-PV2146-01-8804-CH Target Concentration: 0.04 mg/m 3 (arbitrary). There is no OSHA PEL or AGGIH TLV for methotrexate. Procedure: Samples are collected by drawing
More information1,2-Dibromoethane (EDB) and 1,2-dibromo-3-chloropropane (DBCP), gas chromatography, microextraction
1. Application 1,2-Dibromoethane (EDB) and 1,2-dibromo-3-chloropropane (DBCP), gas chromatography, microextraction Parameters and Codes: EDB and DBCP, whole water recoverable, O-3120-90 Parameter (µg/l)
More informationHazard Communication for Dangerous and Harmful Materials
Hazard Communication for Dangerous and Harmful Materials Yu-Li Huang Department of Safety, Health and Environmental Engineering National Kaohsiung First University of Science & Technology 1 Outline Overview
More informationMacrolides in Honey Using Agilent Bond Elut Plexa SPE, Poroshell 120, and LC/MS/MS
Macrolides in Honey Using Agilent Bond Elut Plexa SPE, Poroshell 120, and LC/MS/MS Application Note Food Testing and Agriculture Author Chen-Hao (Andy) Zhai and Rong-jie Fu Agilent Technologies (Shanghai)
More informationAppendix II- Bioanalytical Method Development and Validation
A2. Bioanalytical method development 1. Optimization of chromatographic conditions Method development and optimization of chromatographic parameters is of utmost important for validating a method in biological
More informationBIOO FOOD AND FEED SAFETY. Histamine Enzymatic Assay Kit Manual. Catalog #: Reference #:
BIOO FOOD AND FEED SAFETY Histamine Enzymatic Assay Kit Manual Catalog #: 1032-05 Reference #: 1032-05 BIOO Scientific Corp. 2010 TABLE OF CONTENTS GENERAL INFORMATION... 1 Product Description... 1 Procedure
More informationTECHNICAL TEMEPHOS. 1. Specification. Full specification WHO/SIT/19.R4 Revised 10 December Description
WHO/SIT/19.R4 TEMEPHOS TECHNICAL TECHNICAL TEMEPHOS 1. Specification 1.1 Description Full specification WHO/SIT/19.R4 Revised 10 December 1999 The material shall consist of temephos together with related
More informationPacked Column for Ultra-Fast Reversed-Phase Liquid Chromatography, TSKgel Super-ODS. Table of Contents
No. 089 SEPARATION REPORT Packed Column for Ultra-Fast Reversed-Phase Liquid Chromatography, TSKgel Super-ODS Table of Contents 1. Introduction 1 2. Column Specification 1 3. Features of Packing Materials
More informationRapid Screening and Confirmation of Melamine Residues in Milk and Its Products by Liquid Chromatography Tandem Mass Spectrometry
Rapid Screening and Confirmation of Melamine Residues in Milk and Its Products by Liquid Chromatography Tandem Mass Spectrometry Application Note Food Authors Jianqiu Mi, Zhengxiang Zhang, Zhixu Zhang,
More informationTHE UNIVERSITY OF NEWCASTLE- DISCIPLINE OF MEDICAL BIOCHEMISTRY. STANDARD OPERATING PROCEDURE PROCEDURE NO: GLP 021 MOD: 1st Issue Page: 1 of 6
Page: 1 of 6 1. Risk Assessment: This Risk Assessment is to be used as a general guide and as such, cannot accommodate all the varying factors that may be encountered when using this equipment. Therefore,
More informationMETHOD 8030A ACROLEIN AND ACRYLONITRILE BY GAS CHROMATOGRAPHY
METHOD 8030A ACROLEIN AND ACRYLONITRILE BY GAS CHROMATOGRAPHY 1.0 SCOPE AND APPLICATION 1.1 Method 8030 is used to determine the concentration of the following volatile organic compounds: Compound Name
More informationPerfluorinated Alkyl Acids (PFAA) in Water by LC/MS/MS - PBM
Organics Revision Date: July 19, 2017 Perfluorinated Alkyl Acids (PFAA) in Water by LC/MS/MS - PBM Parameter Perfluorinated Alkyl Acids (Perfluorobutane Sulphonate (PFBS), Perflourooctane Sulphonate (PFOS),
More informationFluoro NADP/NADPH Fluorescent NADP/NADPH Detection Kit
Fluoro NADP/NADPH Fluorescent NADP/NADPH Detection Kit Contact Information Address Telephone Toll Free Fax General Information Sales Technical Questions Website Cell Technology Inc 950 Rengstorff Ave Suite
More informationHuman Lipocalin-2/NGAL ELISA
K-ASSAY Human Lipocalin-2/NGAL ELISA For the quantitative determination of Lipocalin-2/NGAL in human biological samples Cat. No. KT-542 For Research Use Only. 1 Rev. 13453542 K-ASSAY PRODUCT INFORMATION
More informationPYRIPROXYFEN TECHNICAL
WHO/IS/TC/715/2001 TECHNICAL TECHNICAL 1. Specification 1.1 Description Interim specification WHO/IS/TC/715/2001 The material shall consist of pyriproxyfen together with related manufacturing impurities.
More informationGolimumab Free Anti-Drug Antibody ELISA
Golimumab Free Anti-Drug Antibody ELISA For the semi-quantitative determination of free anti-drug antibodies against SIMPONI in human serum and EDTA plasma. For Research Use Only. Not For Use In Diagnostic
More informationVINYL CHLORIDE. Recommended by NIOSH, partially evaluated by OSHA Laboratory.
VINYL CHLORIDE Method no.: 04 Matrix: Air Target concentration: 3 1 ppm (2.5 mg/m ) (OSHA PEL) Procedure: Collection on charcoal (two-tubes in-series), desorption with carbon disulfide, analysis by gas
More informationCreatinine Colorimetric Detection Kit
Creatinine Colorimetric Detection Kit For the quantitative determination of creatinine in urine. For Research Use Only. Not For Use In Diagnostic Procedures. Catalog Number: 74-CRNHU-E02 Size: 2x96 Wells
More informationCERTIFICATE OF ANALYSIS Nitric Oxide Assay Kit
Ordering Code: EMSNO CERTIFICATE OF ANALYSIS Nitric Oxide Assay Kit Lot Number: MG157087 Product: A complete kit for the quantitative determination of nitrite and nitrate in biological fluids (2 Plate,
More informationFigure 1. Structures for Vitamin B2 and Vitamin B1.
CH 461 & CH 461H F 15 Name Experiment 2C Integrated Laboratory Experiment DETERMINATION OF RIBOFLAVIN: A COMPARISON OF TECHNIQUES PART C. HIGH PERFORMANCE LIQUID CHROMATOGRAPHY The purpose of this experiment
More informationMouse Creatinine Urinary Detection Kit
Mouse Creatinine Urinary Detection Kit Catalog No: IRAAKT2549 Lot No: SAMPLE INTENDED USE BACKGROUND Creatinine (2 amino 1 methyl 5H imadazol 4 one) is a metabolite of phosphocreatine (p creatine), a molecule
More informationDetermination of Volatile Substances Proof of Food Adulteration
ANALYSIS OF FOOD AND NATURAL PRODUCTS LABORATORY EXERCISE Determination of Volatile Substances Proof of Food Adulteration (method: gas chromatography with mass spectrometric detection) Exercise guarantor:
More informationDetermination of Polymer Modifier in Asphalt
Standard Method of Test for Determination of Polymer Modifier in Asphalt AASHTO Designation: T xxx-xx (2005) 1. SCOPE 1.1. This method of test is used to determine the polymer content of an asphalt sample.
More informationMouse Lipocalin-2/NGAL ELISA
K-ASSAY Mouse Lipocalin-2/NGAL ELISA For the quantitative determination of lipocalin-2/ngal in mouse biological samples Cat. No. KT-662 For Research Use Only. 1 Rev. 10747500 K-ASSAY PRODUCT INFORMATION
More informationRat Ferritin ELISA. For the determination of ferritin in serum and plasma of rats. For Research Use Only. Not For Use In Diagnostic Procedures.
Rat Ferritin ELISA For the determination of ferritin in serum and plasma of rats. For Research Use Only. Not For Use In Diagnostic Procedures. Catalog Number: 41-FERRT-E01 Size: 96 Wells Version: 3 L52-16
More informationCanine brain natriuretic peptide,bnp ELISA Kit
Canine brain natriuretic peptide,bnp ELISA Kit Catalog No: E0541c 96 Tests Operating instruction www.eiaab.com FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH
More informationGlutamate Dehydrogenase Assay Kit
Glutamate Dehydrogenase Assay Kit Catalog Number KA0879 100 assays Version: 03 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information... 4
More informationFigure 1. Structures for Vitamin B2 and Vitamin B1.
CH 461 & CH 461H F 18 Name Experiment 2C Integrated Laboratory Experiment DETERMINATION OF RIBOFLAVIN: A COMPARISON OF TECHNIQUES PART C. HIGH PERFORMANCE LIQUID CHROMATOGRAPHY The purpose of this experiment
More informationAmphetamines, Phentermine, and Designer Stimulant Quantitation Using an Agilent 6430 LC/MS/MS
Amphetamines, Phentermine, and Designer Stimulant Quantitation Using an Agilent 643 LC/MS/MS Application Note Forensic Toxicology Authors Jason Hudson, Ph.D., James Hutchings, Ph.D., and Rebecca Wagner,
More informationExtraction of Methylmalonic Acid from Serum Using ISOLUTE. SAX Prior to LC-MS/MS Analysis
Application Note AN89.V.1 Extraction of Methylmalonic Acid from Serum Using ISOLUTE SAX Page 1 Extraction of Methylmalonic Acid from Serum Using ISOLUTE SAX Prior to LC-MS/MS Analysis Sample Preparation
More informationApplication Note. Abstract. Introduction. Experimental-Instrument Conditions. By: Anne Jurek
Automated Handling Techniques for the Analysis of Elevated Volatile Organic Compound (VOC) Concentrations in Soils Utilizing the Atomx Concentrator/Multimatrix Autosampler. Application Note By: Anne Jurek
More informationHigh Performance Liquid Chromatography (HPLC) of Three Analgesics
High Performance Liquid Chromatography (HPLC) of Three Analgesics OVERVIEW/THEORY Real-life samples are often complex mixtures of many substances (e.g., sea water contains many salts and other impurities
More informationHuman Alpha 1-Anti- Chymotrypsin ELISA
K-ASSAY Human Alpha 1-Anti- Chymotrypsin ELISA For the quantitative determination of Alpha 1-Anti-Chymotrypsin in human biological fluids Cat. No. KT-498 For Research Use Only. Not for use in diagnostic
More informationATP Fluorometric/Colorimetric Assay Kit
ATP Fluorometric/Colorimetric Assay Kit Catalog No. KM0028 Detection and Quantification of ATP Concentrations in Biological Samples. Research Purposes Only. Not Intended for Diagnostic or Clinical Procedures.
More informationCat IgA ELISA. Cat. No. KT-755 K-ASSAY KAMIYA BIOMEDICAL COMPANY. For the quantitative determination of IgA in cat biological samples
K-ASSAY KAMIYA A BIOMEDICAL COMPANY KAMIYA BIOMEDICAL COMPANY Cat IgA ELISA For the quantitative determination of IgA in cat biological samples Cat. No. KT-755 For research use only. 1 Rev. 11607093 K-ASSAY
More informationHuman Leptin ELISA. Cat. No. KT-1120 K-ASSAY. For the quantitative determination of leptin in human biological samples. For research use only.
K-ASSAY Human Leptin ELISA For the quantitative determination of leptin in human biological samples Cat. No. KT-1120 For research use only. 1 Rev. 11018704 K-ASSAY PRODUCT INFORMATION Human Leptin ELISA
More informationMouse IgE ELISA. Cat. No. KT-401 K-ASSAY. For the quantitative determination of IgE in mouse biological samples. For Research Use Only.
K-ASSAY Mouse IgE ELISA For the quantitative determination of IgE in mouse biological samples Cat. No. KT-401 For Research Use Only. 1 Rev. 13328401 K-ASSAY PRODUCT INFORMATION Mouse IgE ELISA Cat. No.
More informationMouse Glutathione S Transferase Alpha 1 (GSTa1)
Mouse Glutathione S Transferase Alpha 1 (GSTa1) For the quantitative determination of mouse (GSTa1) in serum, plasma and other biological fluids Cat. No. KT-16414 For Research Use Only. Not for use in
More informationRat Alpha Glutathione S- Transferase ELISA
K-ASSAY Rat Alpha Glutathione S- Transferase ELISA For the quantitative determination of alpha glutathione s-transferase in rat biological samples Cat. No. KT-632 For Research Use Only. 1 Rev. 13571632
More informationUncontrolled Copy. SOP 109 Ethylene Glycol Screen by Gas Chromatography/Mass Spectrometry. Table of Contents. 1. Principle of Assay...
Table of Contents 1. Principle of Assay... 3 2. Specimens... 3 3. Reagents and Materials (HPLC grade)... 3 4. Standards, Controls, and Solutions... 4 5. Equipment and Special Supplies... 5 6. Instrumentation...
More informationGlutathione HPLC Kit. For the determination of glutathione in EDTA whole blood KC Manual. Valid from
Distribuito in ITALIA da Li StarFish S.r.l. Via Cavour, 35 20063 Cernusco S/N (MI) telefono 02-92150794 fax 02-92157285 info@listarfish.it www.listarfish.it Manual HPLC Kit For the determination of glutathione
More informationOperating Instructions
Sequazyme Peptide Mass Standards Kit Operating Instructions 1 Product Description The Sequazyme Peptide Mass Standards Kit includes reagents needed to test instrument function, optimize instrument parameters,
More informationTHE UNIVERSITY OF NEWCASTLE- DISCIPLINE OF MEDICAL BIOCHEMISTRY. STANDARD OPERATING PROCEDURE PROCEDURE NO: GLP 019 MOD: 1st Issue Page: 1 of 6
Page: 1 of 6 1. Risk Assessment:. This Risk Assessment is to be used as a general guide and as such, cannot accommodate all the varying factors that may be encountered when using this equipment. Therefore,
More informationD-Lactate Fluorometric/Colorimetric Assay Kit
D-Lactate Fluorometric/Colorimetric Assay Kit Catalog No. KM0096 Detection and Quantification of D-Lactate Concentrations in Biological Samples. Research Purposes Only. Not Intended for Diagnostic or Clinical
More informationAflatoxin M1 (AFM1) ELISA Kit
Aflatoxin M1 (AFM1) ELISA Kit Catalog Number. CSB-EL027236 This immunoassay kit allows for the in vitro quantitative determination of Aflatoxin M1 concentrations in milk, milk power. This package insert
More informationU.S. Patent No. 9,051,563 and other pending patents. Ver
INSTRUCTION MANUAL Direct-zol 96 RNA Catalog Nos. R2054, R2055, R2056 & R2057 Highlights Quick, 96-well purification of high-quality (DNA-free) total RNA directly from TRIzol, TRI Reagent and all other
More informationSulfotepp impurities in Chlorpyrifos EC formulations
Page 1 of 16 Method DAS-AM-01-058 Sulfotepp impurities in Chlorpyrifos EC formulations A. ABSTRACT Method DAS-AM-01-058 has been validated for the analysis of the relevant impurity sulfotepp in chlorpyrifos
More informationPhysical Separations and Chromatography
Lab #5A & B: Physical Separations and Chromatography Individual Objectives: At the end of these experiments you should be able to: Ø Distinguish between Rf and tr; chromatograph and chromatogram; adsorption
More informationVALIDATION OF A UPLC METHOD FOR A BENZOCAINE, BUTAMBEN, AND TETRACAINE HYDROCHLORIDE TOPICAL SOLUTION
VALIDATION OF A UPLC METHOD FOR A BENZOCAINE, BUTAMBEN, AND TETRACAINE HYDROCHLORIDE TOPICAL SOLUTION Andrew J. Aubin and Tanya L. Jenkins Waters Corporation, Milford, MA, USA INTRODUCTION Benzocaine (4-Aminobenzoic
More informationMouse Ferritin ELISA
K-ASSAY Mouse Ferritin ELISA For the quantitative determination of ferritin in mouse biological samples Cat. No. KT-396 For research use only. 1 Rev. 13328396 K-ASSAY PRODUCT INFORMATION Mouse Ferritin
More informationMETHOD: 1403, Issue 3 EVALUATION: FULL Issue 1: 15 August 1990 Issue 3: 15 March 2003
ALCOHOLS IV 1403 (1) HOCH 2 CH 2 OCH 3 MW: (1) 76.09 CAS: (1) 109-86-4 RTECS: (1) KL5775000 (2) HOCH 2 CH 2 OCH 2 CH 3 (2) 90.12 (2) 110-80-5 (2) KK8050000 (3) HOCH 2 CH 2 O(CH 2 ) 3 CH 3 (3) 118.17 (3)
More informationRNA Transport. R preps R preps
RNA Transport R0527-00 5 preps R0527-01 50 preps July 2014 RNA Transport Table of Contents Introduction...2 Kit Contents/Storage and Stability...3 Protocol...4 Storage Procedure...4 Recovery Procedure...5
More informationHuman anti-deoxyribonuclease B, anti-dnase B ELISA Kit
Human anti-deoxyribonuclease B, anti-dnase B ELISA Kit Catalog No: E0311h 96 Tests Operating instruction www.eiaab.com FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ
More informationHorse Serum Amyloid A ELISA
K-ASSAY Horse Serum Amyloid A ELISA For the quantitative determination of Serum Amyloid A (SAA) in horse biological fluid Cat. No. KT-660 For research use only. 1 Rev. 13505660 K-ASSAY PRODUCT INFORMATION
More informationCatalase Fluorometric/Colorimetric Assay Kit
Catalase Fluorometric/Colorimetric Assay Kit Catalog No. KM0044 Detection and Quantification of Catalase Concentrations in Biological Samples. Research Purposes Only. Not Intended for Diagnostic or Clinical
More informationELISA CHECK. Application. Introduction. Test principle. ELISA microplate reader accuracy. ELISA microplate linearity
Application PishtazTeb Company ELISA CHECK is used for ELISA washing machine performance verification, accuracy and linearity of microplate reader and also to check precision of micropipettes. This kit
More information