DART Presentation. Brian Musselman BioCop & Confidence Workshop April th ICT Prague

Transcription

1 DART Presentation Brian Musselman BioCop & Confidence Workshop April th ICT Prague

2 The Art of DART Everyone wants to knows what s is in their sample Most methods require significant sample prep to get answers DART simplifies the process by generating simple MS data.

3 The Beginning

4 Formulated Products Utilize Thermal Desorption Technique For small tablets you can subsequently load up to 10 tablets then analyze for content by DART-MS in under 2 minutes Vary the carrier gas temperature to thermally profile the product Small tablets are held it place by a spring loaded plate pushing the tablets against a template Large tablets can simply be placed in the tablet tray

5 DART-SVP with Angle Insert the Tablet Carrier onto the ET (Experiment Table) Vary the carrier gas temperature while scanning across the surface to thermally desorb layer after layer of product Operation at up to 45 degree angle for efficient desorption

6 Thermal Desorption Method Development Daytime Caplet 4 DART-ET Temperatures - Search for 3 Active Ingredients A 150 ºC A D 250 ºC A P D 350 ºC A P D 450 ºC

7 Night time Caplet DART-ET 350 ºC Dextromethorphan HBr 1 Chlorine Isotope Pattern Chlorpheniramine Maleate 0.3%

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10 DART Analysis of Raw Milk C:\Xcalibur\...\stds_x2_350C_25k :59:12 stds_x2_350c_25k #663 RT: 2,33 AV: 1 NL: 2,31E5 T: FTMS {0;0} + p NSI Full ms [55,00-400,00] Dimethyl furfural Interference 127,0390 MEL [M+H] ppm 127, , ppb MELAMINE SPIKED LIQUID 0 126,90 126,95 127,00 127,05 127,10 127,15 127,20 127,25 C:\Xcalibur\...\stds_x2_350C_25k :59:12 m/z stds_x2_350c_25k #931 RT: 3,23 AV: 1 NL: 1,86E5 T: FTMS {0;0} + p NSI Full ms [55,00-400,00] Interference 127, ppb MEL [M+H] ppm LOD ~ 70ppb , , ,90 126,95 127,00 127,05 127,10 127,15 127,20 127,25 m/z

11 Matrix Effects Water vs Milk 2_5ppm_solvent_stdx3_matrix_stdx3_ :28:48 RT: 1,20-3, STANDARD SM: 7B IN WATER (melamine 2.5ppm) 1,73 2,00 NL: 1,18E7 m/z= 127,06-127,08 MS 2_5ppm_solvent _stdx3_matrix_st dx3_350c_25k 70 1,40 1, RAW MILK (melamine 2.5ppm) ,36 2,64 2,66 2,67 2,96 0 1,2 1,4 1,6 1,8 2,0 2,2 2,4 2,6 2,8 3,0 Time (min)

12 Rapid Analysis of Liquids For liquids you can analyze 12 samples per minute at high speed for qualitative analysis Or slow the presentation speed to deliver more quantitative analysis Analysis of 64 samples of Virgin Olive Oil in 22 minutes looking for di- and triglyceride content

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14 High Throughput with No Sample Prep 48 Samples in under 20 minutes

15 Oils Positive-Ion Spectral Extra Virgin Olive Oil Fingerprints 100% Pure Olive Oil Mild Olive Oil Pure Vegetable Oil Toasted Sesame Oil

16 Oils Negative-Ion Spectral Fingerprints Extra Virgin Olive Oil 100% Pure Olive Oil Pure Vegetable Oil Toasted Sesame Oil

17 Raw Materials The Tweezer Block permits analysis of irregular shaped objects Or determine the content of active ingredients in seconds

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19 Simple Sample Preparation Mix Metal with Powder Position in Desorption Ionization Region

20 What does this look like? Magnetic Field to High

21 Acquiring the Sample? Magnetized nail insert into vial

22 Scanning Multiple Tips Array of Magnetized nails in 96 well format

23 Tylenol Cold Tablet Nighttime Caplet Acetaminophen 325 mg/caplet Phenylephrine HCl 5 mg/caplet Chlorpheniramine Maleate 2 mg/caplet

24 10% Tylenol Powder in Metal TIC irregular Mass chromatogram for each API s have consistent ratios Acetaminophen = A Phenylephrine = P Chlorpheniramine Maleate = C Relative abundance vary from sample to sample

25 Mustard Powder Mix 1% Mustard Powder with Magnetite Vortex 30 seconds Deposit 3mg aliquot on glass slide Analyze 450C 350C

26 Mustard Powder Replicat e analyses Intact triglycerid es at 450C 450C

27 Thyme Leaves Positive Ion MS 350 degree Centigrade Helium gas Negative Ion MS

28 Thyme Leaves MS and MS/MS

29 McCormick Curry Powder Positive Ion MS 350 degree Centigrade Helium gas Negative Ion MS

30 Expanding DART s Range Desorption is typically protonated molecule for positive ion and deprotonated for negative ion Thermally Stable Molecules Derivatized Molecules Making stable molecules in the DART experiment

31 Crushed TIC TAC Disaccharide?

32 Korean Ginseng from Capsule Mix metal w/powder - add TMAH

33 Ginsenioside Rb 1 THAH Derivative Sigma Standard 1 mg dispersed in metal 3 mg on slide Apply 5 ul 25% solution TMAH in methanol Analyze at 350C to complete insitu derivative

34 Ginsenioside Rb 1 MS/MS 1364

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36 Utility of High Resolution for Very Rapid Analysis of Complex Mix Pesticide Mix 529

37 Automation of DART Analysis + + Need automated sampling mechanism Need efficient, less labor intensive data analysis

38 AUTOMATION HARDWARE DART ionization source positioned under the rail of a programmable CTC autosampler from LEAP Technologies DART sources is standard production Injector body is modified to accept a new mandrel capable of picking up pipettor tips Standard 96-well titer plates used to hold samples Custom tips developed to create more reliable sample positioning in the ionization zone Implementation of Thermo LC/MS permits integrates of sample information with Xcalibur software Customizable sampling routines can be completed

39 SYSTEM CONFIGURATION

40 TIP ACQUISITION

41 SAMPLING FROM PLATE

42 Applications of DART in Prep-HPLC R1 It was never separated on the prep-column Which fraction contains the bromo imp? Results Courtesy of W. Schafer, Merck, Ambient Pressure Desorption Ionization Mass Spectrometry In Support of Preclinical Pharmaceutical Development, submitted 10/2009

43 96 Well Plate Analysis CH 3 N N N H Exact Mass: TIC oxidative enzymes SM (M+1=134) Prod (SM+16)

44 area DART Linearity on Agilent MSD M+1 extracted from scan SIM (M+1) = mg/ml MW 220 Sensitive enough for to track product formation Semi-quantitative: allows chemist to quickly identify best hits on plate

45 DART-SVP 2-D Scanner Experimental Program and Results IonSense, Inc. December 4, 2009

46 2D Scanner for DART-SVP Domestic & Foreign Currencies

47 2D Scanning Method: Example Bank Note - Royal Bank of Penguins START END 10 scans total across the bank note with 1 cm spacing row to row Linear rail speed was set at 1 mm/s Could not scan the entire length of the bank notes with the current set-up

48 Well Circulated $1 Bill Bottom Right-hand Corner Cocaine easily detected off of the $1 bill at an intensity level of 7.7 E6.

49 $10 Used vs. New - Bottom Right-hand Corner USED $10 Bill NEW $10 Bill No presence of cocaine on the crisp $10 bill

50 Well Circulated $20 Bill Varying Cocaine Signal Top Right Center Bottom Right

51 Circulated 20 Note Fingerprint Ions Center Middle Right Bottom Right

52 Circulated 200 Czech Korunas Note Fingerprint Ions Dense Ink Area on Image of Jan Amos Komensky Bottom Right Corner

53 Conclusions IPOD Touch controlled Direct Analysis in Real Time provides a methods driven platform for solving problems