DUS Characterization of Narendra Lahar: A New High Yielding Rice Variety

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1 Int.J.Curr.Microbiol.App.Sci (0) 6(): - International Journal of Current Microbiology and Applied Sciences ISSN: -06 Volume 6 Number (0) pp. - Journal homepage: Original Research Article DUS Characterization of Narendra Lahar: A New High Yielding Rice Variety S.P. Giri*, Alok Pandey, V.N. Singh, D.K. Verma, R.M. Tripathi, Arun Kumar Singh, M.L. Maurya and R.V. Singh Crop Research Station, Masodha (N.D. University of Agriculture and Technology), Post Dabhasemer, Faizabad - (U.P.), India *Corresponding author A B S T R A C T K e y w o r d s Yielding, Uniformity, Novelty, Varieties. Article Info Accepted: October 0 Available Online: 0 November 0 Information regarding Novelty, distinctness, uniformity and stability are the basic mandates for the protection of varieties under Protection of Plant Varieties and Farmers' Rights Act (PPVFRA), 00. A field experiment was conducted at Crop Research Station, Masodha Faizabad for DUS characterization of recently released high yielding rice variety Narendra Lahar as per the guidelines of International Union for the Protection of New Varieties of Plants (UPOV) during kharif 0. DUS test results shows that Narendra Lahar is medium maturing, maturing, good tillering ability and semi tall rice variety posses. The basal leaf sheath colour is green in coloration while leaf anthocynincolration is absent in the variety. Leaf: pubescence of blade surface is very week and colorless auricles were present in the variety. Ligule was present and green in color. The grain is long slender in shape having intermediate amylase content. Molecular characterization using SSR markers also exhibits distinctness of new variety. Introduction Rice is the staple food of more than / rd population of the world and one of the largest cultivated cereal crops. It is also the major source of nutrition and livelihood of more than 0-0 million rural households of the country and backbone of agriculture based economy of India. At 0 million tons, it accounts for % of food grain production and % of cereal production at present. At current rate of population of.8% per annum, the rice requirement of the country is estimated to be around 0-60 million tonnes by 00 (,,). Achieving this target in the next few decades, without harming the environment would be a great challenge. Uttar Pradesh is the nd largest producer of rice in the country just after West Bengal. Eastern part of the Uttar Pradesh is the hub of rice production and rice is grown in almost all the ecologies viz. rainfed upland, well managed irrigated and rainfed lowland due highly fertile soil of Indo Gangetic plain. The major factors affecting rice productivity in this region is low coverage by ecosystem specific varieties/hybrids, degrading soil fertility/health, erratic mansoon rainfall, shortage of labours and timely supply of other inputs. Development of new rice variety specific to prevalent ecology and agro climatic conditions is one of the options to enhanced the productivity of this region (,). In view of the above observation Narendra

2 Int.J.Curr.Microbiol.App.Sci (0) 6(): - Lahar (NDR 0) a new high yielding rice variety was developed at Crop Research Station, Masodha for favourable irrigated ecology of Uttar Pradesh. NDR 0 was developed from triple cross of IR /Janak//IRRI 0. This entry was tested in Regional Agriculture Demonstration & Testing Station, Uttar Pradesh (RATDS) for consecutively three years from The performance of NDR 0 is promising in all the zones viz. central, eastern, Northern and western zones of Uttar Pradesh (6,,8). This variety was found resistant to major location specific pests viz. Stem borer and Leaf folder and also found moderately resistant to major diseases (Blast, Sheath Blight and Bacterial Leaf Blight). The head rice recovery (HRR) of Narendra Lahar was quantified 6.80%. Agronomical evaluation of NDR 0 indicated good fertilizer responsive trait of this rice variety. The yield capacity of this variety of irrigated medium duration is -60 q/ha. Performance of the NDR 0 in On Farm and Front Line Demonstartions was also very encouraging and it out yielded popular rice varieties of area in yield. NDR 0 also established its yield superiority over most popular variety NDR and may be good replacement for this variety as it has synchronous flowering which NDR lacks. Information regarding Novelty, distinctness, uniformity and stability are basic requirement for the protection of varieties under Protection of Plant Varieties and Farmer s Rights ACT (PPVFRA), 00. Hence, the molecular and morphological characteristics of the Narendra Lahar (NDR 0) were studied for DUS characterization of the variety. Materials and Methods DUS characterization of Narendra Lahar The DUS characterization of the Narendra Lahar (NDR 0) was carried out at Crop Researcha Station, Masodha, Faizabad during kharif 0. The soil of the experimental field was sandy loam in texture with very low organic carbon (0.%). The ph of the soil was found.. Nitrogen content of the soil was 00 kg/ha, available PO is kg/ha and available KO was kg/ha. The experiment was laid down in randomized block design with three replications. The plot size of the experimental plots was sqm and spacing of 0 cm between rows and cm between plants was maintained to grow the crop. Recommended package of practices were adopted to raise the crop. Observations on agro morphological traits were recorded at different s of the crop for the DUS characterization of the Variety as per the national guidelines for DUS test in rice (Table ). Molecular characterization of Narendra Lahar DNA isolation and PCR assays The genomic DNA was extracted from 0 days old rice seedlings as per Dellaporta et al., (). PCR analysis was performed with 0. iltaq DNA polymerase (U/il) (Biotools), il of genomic DNA 0 ng/il, il of 0X buffer (Biotools), 0. il of dntps (. mm) and il of each primer pair in a total volume of 0 il. PCR was performed using thermal cycler (Applied Biosystems) following the PCR protocols reported earlier with necessary modifications. The amplified products were separated in % metaphor gel and visualized under phospho imager system after staining with ethidium bromide. The sizes of the amplified fragments were estimated visually using 00bp DNA ladder as size standard. Only clear and unambiguous bands of markers were scored. Band position of the prominent markers was depicted in table and figure.

3 Int.J.Curr.Microbiol.App.Sci (0) 6(): - Table. DUS characteristics of Narendra Lahar (NDR 0) S.No. Characteristics States Note Stage of observation Type of assessment Coleoptile: colour Colourless Green Germination. Basal leaf: sheath colour Leaf: intensity of green colour Leaf: anthocyanin colouration Leaf : distribution of anthocyanin colouration 6 Leaf sheath: anthocyanin colouration Leaf sheath : intensity of anthocyanin colouration 8 0 Leaf: pubescence of blade surface Leaf : auricles Leaf: anthocyanin colouration of auricles Green Light purple lines Light Dark On tips only On margins only In blotches only Uniform Very weak Colourless Light purple Leaf: collar Leaf: anthocyanin colouration of collar Leaf: ligule Leaf: shape of ligule Truncate Acute. Leaf: colour of ligule Split Green Light purple 6 Leaf: length of blade Short Long Leaf: width of blade Narrow Broad 6

4 Int.J.Curr.Microbiol.App.Sci (0) 6(): - S.No. Characteristics States Note Stage of observation 8 Culm: attitude (for floating Non procumbent rice only) Procumbent 0 Culm: attitude Time of heading (0% of plants with panicles) Flag leaf: attitude of blade (early observation) Spikelet: density of pubescence of lemma Male sterility Lemma: anthocyanin colouration of keel Lemma: anthocyanin colouration of area below apex Lemma: anthocyanin colouration of apex Spikelet: colour of stigma Stem: thickness Stem: length (excluding panicle; excluding floating rice) Stem: anthocyanin colouration of nodes Erect Semi-erect Open Spreading Very early (< days) Early (-0 days) (-0 days) Late (-0 days) Very late (>0 days) erect semi-erect horizontal deflexed or very weak White Light green Yellow Light purple Thin Thick Very short (< cm) Short (-0 cm) (-0 cm) Long (-0 cm) Very long (>0 cm) Type of assessment Maturity Maturity Maturity

5 Int.J.Curr.Microbiol.App.Sci (0) 6(): - S.No. Characteristics States Note Stage of observation Stem : intensity of Maturity anthocyanin colouration of nodes Stem: anthocyanin colouration of internodes Panicle: length of main axis Flag leaf: attitude of blade (late observation) Panicle: curvature of main axis Very short (<6 cm) Short (6-0 cm) (- cm) Long (6-0 cm) Very long (>0 cm) Erect Semi-erect Horizontal Deflexed Straight Semi-straight Drooping Deflexed 6 Panicle: number per plant Few (<) (-0) Many (>0) Spikelet : colour of tip of lemma White Yellowish Brown Red Black 8 Lemma and Palea: colour Straw Gold and gold furrows on straw background Brown spots on straw Brown furrows on straw Brown (tawny) Reddish to light purple spots on straw furrows on straw Black 0 Panicle : awns Panicle: colour of awns (late observation) Panicle: length of longest awn Yellowish white Yellowish brown Brown Reddish brown Light red Red Light purple Black Very short Short Long Type of assessment Maturity Maturity Maturity Maturity Maturity Maturity Maturity Maturity Maturity - Maturity -

6 Int.J.Curr.Microbiol.App.Sci (0) 6(): - S.No. Characteristics States Note Stage of observation Type of assessment Very long Panicle: distribution of Tip only Maturity - awns Upper half only Whole length Panicle: presence of Maturity. 6 secondary branching Panicle: secondary branching Panicle: attitude of branches Panicle: exsertion Clustered Erect Erect to semi-erect Semi-erect Semi-erect to spreading Spreading Partly exserted Exserted Well exserted Time of maturity Very early Early Late Very late 8 Leaf: senescence Sterile lemma: colour 0 Grain: weight of 000 fully developed grains Grain: length Grain: width Grain: phenol reaction of lemma Decorticated grain: length Early Late Straw Gold Red Very low Low High Very high Very short Short Long Very long Very narrow Narrow Broad Very broad Very short Short Long Very long Maturity Maturity Maturity Maturity Maturity Maturity Maturity Maturity Maturity Maturity Maturity

7 Int.J.Curr.Microbiol.App.Sci (0) 6(): - S.No. Characteristics States Note Stage of observation Type of assessment Decorticated grain: width Narrow (<.0 mm) (.0-. mm) Maturity Decorticated grain: shape (in lateral view) Decorticated grain: colour Endosperm: presence of amylose Endosperm: content of amylose Varieties with endosperm of amylose absent only Polished grain : expression of white core Gelatinization temperature through alkali spreading value. Decorticated grain: aroma Broad (>. mm) Short slender Short bold slender Long slender Long bold Extra long slender White Light brown Variegated brown Dark brown Light red Red Variegated purple Dark purple Very low (<0%) Low(0-%) (0-%) High(6-0%) Very high (>0%) or very small Smal Large Low High medium High Maturity Maturity Maturity Maturity Maturity - Maturity Maturity Table. Band position of prominent markers in Narendra Lahar SN. Markers Forward Sequence Reverse Sequence bp RM AATCCAAGGTGCAGAGATGG CAACGATGACGAACACAACC 0 RM GCGAAAACACAATGCAAAAA GCGTTGGTTGGACCTGAC 0 RM8 GGCATGAGAGTCTGTGATGTTGG TAGTACTGCTCCATCTGCCTTGG 60 RM TGGAGTTTGAGAGGAGGG CTTGTTGCATGGTGCCATGT 00 RM GTATGCATATTTGATAAGAG AAGTCACCGAGTTTACCTTC 00 6 RM TGCAACTTCTAGCTGCTCGA GCATCCGATCTTGATGGG 0 RM GCTTGCTTGTATCTGCATTGGTAGG GGGATGATCCACTCTCTGTTTGG 60 8 RM GACGGTGACGCACTTTATGAACC CGATCTGCGAGAAACCCTCTCC 0 RM CTGATCGAGAGCGTTAAGGG GGGATCAAACCACGTTTCTG 0 0

8 Int.J.Curr.Microbiol.App.Sci (0) 6(): - SN. Markers Forward Sequence Reverse Sequence bp 0 RM8 ACTTGAGACGATCGGACACC TCACCCATGGATGTTGTCAG 0 OSR- CATTTGTGCGTCACGGAGTA AGCCACAGCGCCCATCTCTC 00 RM8 CACAGGAGCAGGAGAAGAGC GGCAAACCGATCACTCAGTC 00 RM CCGTCGCCGTAGTAGAGAAG TCCCGGTTATTTTAAGGCG 0 RM AGATTGATCTCCCATTCCCC CACGAGCATATTACTAGTGG 80 RM0 GTACTACCGACCTACCGTTCAC CTGCTATGCATGAACTGCTC 0 6 RM ATCGTCTGCGTTGCGGCTGCTG CATGGATCACCGAGCTCCCCCC 00 RM0 TGCCCATGTATGTGAGGTACTCC GCCTAATCCAGGACAAGCTACGG 00 8 RM CCAATCTTGTCTTCCGGATCTTGC AGATAGCCATGGGCGATTCTTGG 0 RM6 TGCAGATGAGAAGCGGCGCCTC TGTGTCATCAGACGGCGCTCCG 00 0 RM8 TCGCGTGAAAGATAAGCGGCGC GATCACCGTTCCCTCCGCCTGC 0 RM GTTCAGTGTTCAGTGCCACC GACTTTGATCTTTGGTGGACG 00 RM TTGGATTGTTTTGCTGGCTCGC GGAACACGGGGTCGGAAGCGAC 0 RM0 AACCGGATTAGTTTCTCGCC TGAGGACGACGAGCAGATTC 0 RM CTCAAGCTTAGCTGCTGCTG GTGATCAGTGCACCATAGCG 00 RM6 GCCAGCAAAACCAGGGATCCGG CAAGGTCTTGTGCGGCTTGCGG 0 6 RM ATCAGCAGCCATGGCAGCGACC AGGGGATCATGTGCCGAAGGCC 0 RM TCTCCTCTTCCCCCGATC ATAGCGGGCGAGGCTTAG 0 8 RM CCACAAATTAATCCGGATCACACC AGCATTGTGCAATCACGAGAAGG 0 RM8 CCAATCGGAGCCACCGGAGAGC CACATCCTCCAGCGACGCCGAG 0 0 RM08 CAACGAGCTAACTTCCGTCC ACTGCTACTTGGGTAGCTGACC 0 RM GAAACCACCACACCTCACCG CCGTAGACCTTCTTGAAGTAG 0 RM ACGGGCAATCCGAACAACC TCGGGAAAACCTACCCTACC 0 RM8 ACTGCATGATCCTCCTCAGATCC CCCTCTGATCTCTGATACTCCATCC 0 RM TGCGCTGAACTAAACACAGC AGACAAACCTGGCCATTCAC 0 RM CCCTTGTGCTGTCTCCTCTC ACGGGCTTCTTCTCCTTCTC 0 6 RM6 CTAGTTGGGCATACGATGGC ACGCTTATATGTTACGTCAAC 0 RM0 GTCGTCGACCCATCGGAGCCAC TGGTCGAGGTGGGGATCGGGTC 0 8 RM CAAAATGGAGCAGCAAGAGC TGAGCACCTCCTTCTCTGTAG 0 RM AAGATGTACGGGTGGCATTC TATGAGCTGGTGAGCAATGG 0 0 RM TCAGATCTACAATTCCATCC TCGGTGAGACCTAGAGAGCC 0 RM AACGCGAGGACACGTACTTAC ACGAGATACGTACGCCTTTG 0 RM8 TCTCCCTCCTCACCATTGTC TGCTGCCCTCTCTCTCTCTC 00 RM CGCAGTTGTGGATTTCAGTG TGCTCAACGTTTGACTGTCC 80 RM6 TCTCTCCTCTTGTTTGGCTC ACACACCAACACGACCACAC 0 RM8 TTCCCTGTTAAGAGAGAAATC GTGTATTTGGTGAAAGCAAC 0 6 RM TGCCCTGGCGCAAATTTGATCC GCTAGAGGAGATCAGATGGTAGTGCATG 0 RM CAAAAACAGAGCAGATGAC CTCAAGATGGACGCCAAGA 60 8 RM CGGTCAAATCATCACCTGAC CAAGGCTTGCAAGGGAAG 0

9 Int.J.Curr.Microbiol.App.Sci (0) 6(): - Fig. Major Markers and Band positions in Narendra Lahar (NDR 0) Results and Discussion The data depicted in table exhibit that Narendra Lahar posse s distinct, unique and stable morphological trait which differ it from the other high yielding varieties of irrigated ecosystem and land races. DUS test evaluation revealed that Narendra Lahar is medium maturing, maturing, good tillering ability and semi tall rice variety. The basal leaf sheath colour is green in coloration while leaf: anthocynincolration is absent in the variety. Leaf: pubescence of blade surface is very week and colorless auricles were present in the variety. Ligule was present and green in color. The anthocynin coloration of nodes and internodes is absent. It possesses aweless and well exerted panicle. The length of the panicle is medium. The 000 grain weight of Narendra Lahar is medium and shape of grain is long slender.. It possesses good cooking quality with moderate amylose content.6% having soft gel (Gel consistency - 6 mm). Cooked rice of Narendra Lahar is non sticky rice varieties are preferred by the Indian consumers. Gelatinization temperature of the variety was also reported on the basis of alkali spreading value and it was found medium. The band position of prominent SSR markers depicted in table and figure also exhibits distinct and uniqueness of this variety. These SSR markers were used by Shakil et al., (0) for molecular characterization of modern high yielding rice varieties of Bangladesh. The present study of molecular and morphological characterization of Narendra Lahar will be helpful in the identification of variety and to maintain its genetic purity. This information will be very useful for the persons involved in seed certification and seed production programme. Acknowledgement The authors would like to thanks Dr. T. Ram, Principal Scientist (Plant Breeding), IIRR, Hyderabad for providing DNA fingerprinting facility. References. Anonymous. 00. Protection of Plant Varieties and Farmers Right Act (No. of 00). Dept. of Agriculture and Cooperation Ministry of Agriculture, Govt. of India, Krishi Bhawan, New Delhi.. Chakravorty, A., Ghosh, P. D. 0. Characterization of Landraces of rice following DUS guidelines. Research in Plant Biology (6): Chakrabarty, S. K., Monika A. Joshi, Yogendra Singh, Aniruddha Maity, Veena Vashisht and Dadlani, M. 0. Characterization and evaluation of variability in farmers varieties of rice from West Bengal. Indian journal of Genetics and Plant Breeding (): 6-.. Chang TT, Bardenas EA, 6. The Morphology and Varietal Characteristics

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