Nature Protocols: doi: /nprot Supplementary Figure 1
|
|
- Sheila Charles
- 5 years ago
- Views:
Transcription
1 Supplementary Figure 1 Photographs of the 3D-MTC device and the confocal fluorescence microscopy. I: The system consists of a Leica SP8-Confocal microscope (with an option of STED), a confocal PC, a 3D-MTC PC and a 3D-MTC; II to V: the steps of loading a cell-containing sample; VI: the current (magnetic field) control box for 3D-MTC.
2 Supplementary Figure 2 Schematic for the three-dimensional (3D) coils. (a) Schematic for the auto temperature controller of 3D coils. The auto temperature controller provides a low flow rate of 37 air to mitigate the potential local heating problem. (b) The size of 3D coils and stage. X and Y coils internal diameters are 4.1 cm and their external diameters are 6 cm; Z coils internal diameters are 5.1 cm and their external diameters are 7 cm. The coils thicknesses are 1 cm each. The stage top dimension are 16 cm x 11 cm x 0.5 cm, bottom dimensions are 15 cm x 10 cm x 0.5 cm. (c) Top view of the Z coils and dish. The diameter of the cell culture dish is 3.5 cm. In the top view, the lateral distance from the dish boundary to the Z coil is 0.8 cm.
3 Supplementary Figure 3 Displacement quantification method. (a) Flow chart of the image correlation method (ICM). (b) At each level the displacement field is estimated by using image A and image B. Image B is deformed by the displacement field calculated at the previous level. Succeeding levels have finer spatial resolution and smaller window size. Corr, calculation of cross-correlation; check, checkpoint; interp, interpolation 48. (c) GFP-H2B fluorescence image. Nucleus is outlined with a dashed line. (d) Displacement map image of the same nucleus.
4 Supplementary Figure 4 Nuclear H2B displacements influenced by stress directionality. A different cell from that in Fig. 4. From left to right: bright-field images of the cell, GFP-Histone 2B fluorescence images, and displacement map images of the same murine melanoma B16 cell. Nucleus is outlined with a dashed line. The thick white arrows point to the bead; the red arrows indicate direction of the bead displacement; the color bar indicates the displacement magnitude. (a) No applied stress (non-magnetized); (b) Magnetizing in Z axis and apply a 15 Pa torque (0.1Hz) in Y axis bright-field images; (c) Magnetizing in Z axis and apply a 15 Pa torque (0.1 Hz) in X axis bright-field images; (d) Magnetizing along Z axis and apply a 9 Pa along X axis and 12 Pa along Y axis (the resultant stress is 15 Pa and stress angle is 36.9 ). (e) Magnetizing along Z axis and apply a 10.6 Pa along X axis and 10.6 Pa along Y axis (the resultant stress is 15 Pa and stress angle is 45 ). (f) Magnetizing along Z axis and apply a 12 Pa along X axis and 9 Pa along Y axis (the resultant stress is 15 Pa and stress angle is 53.1 ). The white square in each displacement map is enlarged and presented on the right (the 4 th image in each row) to show the differences of the displacements between each loading direction (a-f) at the same loading magnitude. It is apparent that stress angles (loading directions) are important in resulting major differences in GFP-H2B displacements. The thin white arrow (150 nm) provides a scale for the magnitudes of the red arrows. Image acquisition time is 1 second per image. Scale bars, 7.5 µm. (g) Computed cell stiffnesses (complex moduli) for this cell at different forcing directions.at different forcing directions.
5 Supplementary Figure 5 H2B strains depend on force directions. This is the same cell as in Supplementary Fig. 4. The bulk strains and shear strains were computed from displacement maps of the nucleus in Supplementary Fig. 4. (a) Stress angle of 0. (b) Stress angle of (c) Stress angle of 45. (d) Stress angle of (e) Stress angle of 90. It is obvious that strain maps are different, with the 90 -stress angle generating highest strains.
6 Supplementary Figure 6 Improved displacements quantification via 3D-MTC with STED. A living CHO cell was plated on collagen-1 coated dish overnight. A magnetizing field was applied along Z-axis and a twisting field was applied along X-axis (the short axis of the cell). The top panel is the data of GFP-H2B of the nucleus via a confocal microscope. The bottom panel is the data of the same cell under the same stress conditions quantified with a STED. Parameters for STED are: STED laser power (90 %) is 810 mw; the STED laser is CW (continuous wave) phase pattern; the excitation laser wavelength is 488nm; the STED depletion laser wavelength is 592 nm; scanning method is beam scanning. The scanning speed is 1000 Hz (1000 lines per sec). The 90 % STED power was estimated to be reduced to ~64.8 mw at the objective lens. (a) The GFP-H2B image of whole nucleus. The white box is the enlarged area. The top left of confocal image is the brightfield image of the same cell, the black dot is an RGD-coated magnetic bead. (b) Enlarged GFP-H2B images. (c) Full Width at Half Maximum (FWHM) of the fluorescence images. Confocal FWHM is 251 nm; STED FWHM is 118 nm. (d) Histograms of the fluorescence counts (with a Gaussian fit) at various FWHMs using confocal or STED. FWHM of confocal is ± 1.03 nm, of STED is ± 0.55 nm; mean ± s.e.m., 100 GFP-H2B spots were measured that were near the GFP-LacI spots next to the DHFR gene locus 19. P<0.001 when comparing FWHM of confocal with that of STED. (e) A 15-Pa stress was applied at 0.3 Hz and peak displacement map was computed (displacement). The white box is the enlarged area. (f) Enlarged displacement maps. The black arrow (150 nm) represents the scale for the displacement magnitude. Image acquisition time is 320 millisecond per image. Comparing the white box of H2B fluorescence via STED with that via confocal, one could see better spatial resolution of the GFP-H2B images. FWHM of the PSF is 118 nm, better than the ~185 nm resolution using the re-scan confocal microscopy 1. Comparing the white box of the displacement map via STED with that via confocal, improved displacements were also noticed. Several other cells showed similar trends.
7 Supplementary Figure 7 Lateral resolutions of confocal microscopy and STED. Full Width at Half Maximum (FWHM) of Point Spread Function (PSF) is defined as Lateral resolution. 0%, confocal fluorescence microscopy only; 30%, 30 % depletion laser power of 270 mw (reduced to ~21.6 mw at the objective lens); 60 %, 60 % depletion laser power of 540 mw (reduced to ~43.2 mw the objective lens); 90%, 90 % depletion laser power of 810 mw (reduced to ~64.8 mw at the objective lens). Mean + s.e.m.; n= 11 cells (each data point is an average value from multiple GFP-H2B measurements near the DHFR gene locus in a cell); the same cells were imaged by confocal microscopy first and then by STED. 3 independent experiments. P <0.001 between data from any two laser powers. The line is a fit from the inverse square root law.
8 Supplementary Figure 8 Nuclear H2B fluorescence images and displacement maps under different conditions. This cell is a representative cell in Supplementary Fig. 7. First row: brightfield images. White dashed lines are outlines of the nucleus. White arrow points to the magnetic bead. Second row: H2B fluorescence images. Note that the magnetic bead did not block the optical path because the bead was not in the optical path between the laser and the sample. Third row: H2B displacement maps when 15 Pa stress at Hz was applied. The pink arrow represents the magnitude and direction of the magnetic bead displacement. Bottom row: enlarged displacement maps corresponding to the white boxes above. (a) No-applied-stress (non-magnetized) confocal images. (b-e) Magnetizing in Z axis and applying a twisting field to induce 15 Pa stress at Hz along X-axis (the short axis of the cell); (b) image obtained with confocal microscopy. Full Width Half Maximum (FWHM) of the fluorescence image PSF is 279 nm; (c) image with 30% STED power (~21.6 mw). The FWHM is 198 nm; (d) image with 60% STED power (~43.2 mw). The FWHM is 157 nm; (e) image with 90% STED power (810 mw) (reduced to ~64.8 mw at the objective lens). The FWHM is 124 nm. Image acquisition time is 320 ms per image. Scale bars, 5 µm.
9 Supplementary Figure 9 STED power did not induce magnetic bead displacement. From left to right: bright-field images of the cell, GFP-Histone 2B fluorescence images, displacement map images and enlarged displacement maps. Nucleus is outlined with a dashed line. The thick white arrows point to the magnetized bead; the pink dot indicates the magnitude and direction of the bead displacement (<5 nm, suggesting no additional displacements by STED); the color bar indicates the displacement magnitude. (a) No applied stress in confocal images. The FWHM is 236 nm; (b) the same nucleus in 90% STED power (~64.8 mw) images. The FWHM is 120 nm. Image acquisition time is 320 ms per image. Scale bars, 5 µm.
10 Supplementary Figure 10 System description of 3D-MTC with confocal fluorescence microscopy. The platform of interfacing 3D-Magnetic Twisting Cytometry (3D-MTC) with the confocal fluorescence microscopy (with an option of stimulated emission depletion (STED) nanoscopy) includes three parts: magnetic field generator subsystem, monitoring and analysis subsystem, and observing subsystem. Each subsystem includes hardware equipment, interface board, control interface program or analysis modules.
11 Supplementary Method Quantification of cell stiffness 1) Complex shear modulus is defined as the ratio of shear stress to shear strain and it apply to Eq. (1). G φ Where σ is shear stress, ε is shear strain. 2) Torque per unit bead volume (the specific torque, Γ s )is defined as shown in Eq. (2). (1) s C H (2) Where C is magnetic moment constant of the beads, which may differ from batch to batch, and thus should be calibrated in a viscous fluid of known viscosity for each batch of beads. In the current study, C is calibrated to be 2 Pa/Gauss. H is the magnetic field strength; the coil s strength is measured as 25 Gauss/100 turns of coil, then for 3D-MTC (which has 180 turns of coil for each coil), H = 0.25 Gauss 180 = 45 Gauss. Thus, Torque per unit bead volume(γs) Γ s = C H = 2 Pa/Gauss 45 Gauss = 90 Pa 3) The total torque is defined by Eq. (3), and F is the total shear force acting the bead surface; see Eq. (4) T F D (3) F 2 4 r (4) Where r is magnetic bead s radius, D is magnetic bead s diameter; in this study, D is 4.5 m. Thus, substitute Eq. (4) into Eq. (3), one gets Eq. (5) T σ 4 π 3 2 4π r r 2r Γs 3 (5) Hence we can obtain shear stress with Eq. (5) (one can see that shear stress differs from the specific torque by a factor of 6): s C H 6 6 (6) 4) Cell shear strain is defined as ε by Eq. (7). ε * d d r (7) Where d is the displacement of a magnetic bead. 5) From Eq. (1), Eq. (6), and Eq. (7), we get Eq. (8). σ C H d C H r G (8) ε 6 r 6 d Since the actual shear stress on the cell surface depends on the contact area between the bead and the cell surface, we introduce a, a parameter of the contact area: 1
12 G β G β C H r 6 d (9) For a 40% embedment of the bead, = 2 (see ref. 46 for determining that is based on bead-cell contact area). For example: displacement of the magnetic bead d = 300 nm; r = 2250 nm; C = 2 Pa/Gauss;H = 45 Gauss, one can calculate the complex shear modulus or cell stiffness: β C H r G 6 d = 2 2 Pa/Gauss 45 Gauss 2250 nm/ (6 300 nm) = 225 Pa Reference. 1. De Luca, G.M. et al. Re-scan confocal microscopy: scanning twice for better resolution. Biomed Opt Express. 4, (2013). 2
SUPPLEMENTARY INFORMATION
DOI: 10.1038/NPHOTON.2013.97 Supplementary Information Far-field Imaging of Non-fluorescent Species with Sub-diffraction Resolution Pu Wang et al. 1. Theory of saturated transient absorption microscopy
More informationFile name: Supplementary Information Description: Supplementary Figures, Supplementary Tables and Supplementary References
File name: Supplementary Information Description: Supplementary Figures, Supplementary Tables and Supplementary References File name: Supplementary Movie 1 Description: The movie shows compression behaviour
More informationLIST of SUPPLEMENTARY MATERIALS
LIST of SUPPLEMENTARY MATERIALS Mir et al., Dense Bicoid Hubs Accentuate Binding along the Morphogen Gradient Supplemental Movie S1 (Related to Figure 1). Movies corresponding to the still frames shown
More informationSingle Emitter Detection with Fluorescence and Extinction Spectroscopy
Single Emitter Detection with Fluorescence and Extinction Spectroscopy Michael Krall Elements of Nanophotonics Associated Seminar Recent Progress in Nanooptics & Photonics May 07, 2009 Outline Single molecule
More informationThe aims of this experiment were to obtain values for Young s modulus and Poisson s ratio for
The Cornu Method Nikki Truss 09369481 Abstract: The aims of this experiment were to obtain values for Young s modulus and Poisson s ratio for Perspex using the Cornu Method. A value of was found for Young
More informationModular Microscope Accessory
Modular Microscope Accessory Modular Microscope Accessory SAMPLE STRUCTURE OBSERVATION DHR MMA Key Features Compact, modular design that directly installs to the DHR frame for easy alignment and minimal
More informationSharper low power STED nanoscopy by time gating
Nature Methods Sharper low power nanoscopy by time gating G. Vicidomini,3, G. Moneron,3, K. Y. Han,3, V. Westphal, H. Ta, M. Reuss, J. Engelhardt, C. Eggeling, S. W. Hell, Max Planc nstitute for Biophysical
More informationMapping the mechanical stiffness of live cells with the scanning ion conductance microscope
SUPPLEMENTARY INFORMATION Mapping the mechanical stiffness of live cells with the scanning ion conductance microscope Johannes Rheinlaender and Tilman E. Schäffer Supplementary Figure S1 Supplementary
More informationAdministrative details:
Administrative details: Anything from your side? www.photonics.ethz.ch 1 Where do we stand? Optical imaging: Focusing by a lens Angular spectrum Paraxial approximation Gaussian beams Method of stationary
More informationMicro-Rheology Measurements with the NanoTracker
Micro-Rheology Measurements with the NanoTracker JPK s NanoTracker optical tweezers system is a versatile high resolution force measurement tool. It is based on the principle of optical trapping and uses
More informationUltrafast Dynamics and Single Particle Spectroscopy of Au-CdSe Nanorods
Supporting Information Ultrafast Dynamics and Single Particle Spectroscopy of Au-CdSe Nanorods G. Sagarzazu a, K. Inoue b, M. Saruyama b, M. Sakamoto b, T. Teranishi b, S. Masuo a and N. Tamai a a Department
More informationLaboratory 3: Confocal Microscopy Imaging of Single Emitter Fluorescence and Hanbury Brown, and Twiss Setup for Photon Antibunching
Laboratory 3: Confocal Microscopy Imaging of Single Emitter Fluorescence and Hanbury Brown, and Twiss Setup for Photon Antibunching Jonathan Papa 1, * 1 Institute of Optics University of Rochester, Rochester,
More informationMicro-rheology of cells and soft matter with the NanoTracker
Micro-rheology of cells and soft matter with the NanoTracker Introduction In micro-rheological measurements, the complex deformation or flow of viscoelastic systems under small external forces is investigated.
More informationDynamic Self Assembly of Magnetic Colloids
Institute of Physics, University of Bayreuth Advanced Practical Course in Physics Dynamic Self Assembly of Magnetic Colloids A. Ray and Th. M. Fischer 3 2012 Contents 1. Abstract 3 2. Introduction 3 3.
More informationSupplementary Figures Supplementary Figure 1: Estimation of the error of the number and brightness of molecules in a single cluster; Simulation
Supplementary Figures Supplementary Figure 1: Estimation of the error of the number and brightness of molecules in a single cluster; Simulation (a,c) Relative estimated numbers of molecules ; (b,d) relative
More informationEnhancement of Exciton Transport in Porphyrin. Aggregate Nanostructures by Controlling. Hierarchical Self-Assembly
Electronic Supplementary Material (ESI) for Nanoscale. This journal is The Royal Society of Chemistry 2018 Supporting Information for Enhancement of Exciton Transport in Porphyrin Aggregate Nanostructures
More informationVisualize and Measure Nanoparticle Size and Concentration
NTA : Nanoparticle Tracking Analysis Visualize and Measure Nanoparticle Size and Concentration 30 Apr 2015 NanoSight product range LM 10 series NS300 series NS500 series Dec 13 34 www.nanosight.com NanoSight
More informationAnti-Bunching from a Quantum Dot
Anti-Bunching from a Quantum Dot Gerardo I. Viza 1, 1 Department of Physics and Astronomy, University of Rochester, Rochester, NY 14627 We study the nature of non-classical single emitter light experimentally
More informationThe FEA Code of LASCAD
The FEA Code of LASCAD Konrad Altmann LAS-CAD GmbH Heat removal and thermal lensing constitute key problems for the design of laser cavities for solid-state lasers (SSL, DPSSL etc.). To compute thermal
More informationSupplementary Information. SI Text 1: Derivation and assumptions of the effective temperature model
Supplementary Information SI Text 1: Derivation and assumptions of the effective temperature model We assume that the displacements of intracellular particles are due to passive thermal activity and active
More informationAdaptive Response of Actin Bundles under Mechanical Stress
Biophysical Journal, Volume 113 Supplemental Information Adaptive Response of Actin Bundles under Mechanical Stress Florian Rückerl, Martin Lenz, Timo Betz, John Manzi, Jean-Louis Martiel, Mahassine Safouane,
More informationSUPPLEMENTARY INFORMATION
SUPPLEMENTARY INFORMATION doi:10.1038/nature12791 Supplementary Figure 1 (1/3) WWW.NATURE.COM/NATURE 1 RESEARCH SUPPLEMENTARY INFORMATION Supplementary Figure 1 (2/3) 2 WWW.NATURE.COM/NATURE SUPPLEMENTARY
More informationSUPPLEMENTARY INFORMATION
DETECTION LIMITS IN PHOTOTHERMAL MICROSCOPY Alexander Gaiduk, Paul V. Ruijgrok, Mustafa Yorulmaz, Michel Orrit Institute of Physics, Leiden University, P.O. Box 9504, 300 RA Leiden, The Netherlands SUPPLEMENTARY
More informationSuper-resolution microscopy reveals a LINC complex recruitment at nuclear indentation sites
Supplementary Information Super-resolution microscopy reveals a LINC complex recruitment at nuclear indentation sites Marie Versaevel 1, Jean-Baptiste Braquenier 2, Maryam Riaz 1, Thomas Grevesse 1, Joséphine
More informationSupplementary Information
Supplementary Information Single molecule FRET reveals the energy landscape of the full length SAM I riboswitch Christoph Manz, 1,2 Andrei Yu. Kobitski, 1 Ayan Samanta, 3 Bettina G. Keller 4, Andres Jäschke,
More informationBEAMS AND PLATES ANALYSIS
BEAMS AND PLATES ANALYSIS Automotive body structure can be divided into two types: i. Frameworks constructed of beams ii. Panels Classical beam versus typical modern vehicle beam sections Assumptions:
More informationSupplementary table I. Table of contact angles of the different solutions on the surfaces used here. Supplementary Notes
1 Supplementary Figure 1. Sketch of the experimental setup (not to scale) : it consists of a thin mylar sheet (0, 02 4 3cm 3 ) held fixed vertically. The spacing y 0 between the glass plate and the upper
More information3D SUPER-RESOLUTION FLUORESCENCE MICROSC- OPY USING CYLINDRICAL VECTOR BEAMS
Progress In Electromagnetics Research Letters, Vol. 43, 73 81, 2013 3D SUPER-RESOLUTION FLUORESCENCE MICROSC- OPY USING CYLINDRICAL VECTOR BEAMS Taikei Suyama 1 and Yaoju Zhang 2, * 1 Department of Electrical
More informationFig. 1. Different locus of failure and crack trajectories observed in mode I testing of adhesively bonded double cantilever beam (DCB) specimens.
a). Cohesive Failure b). Interfacial Failure c). Oscillatory Failure d). Alternating Failure Fig. 1. Different locus of failure and crack trajectories observed in mode I testing of adhesively bonded double
More informationFile Name: Supplementary Information Description: Supplementary Figures and Supplementary Note. File Name: Peer Review File Description:
File Name: Supplementary Information Description: Supplementary Figures and Supplementary Note File Name: Peer Revie File Description: Supplementary Fig.1. Complete set of ACFs extracted by FLCS analysis.
More informationRapid formation of size-controllable multicellular spheroids. via 3D acoustic tweezers
Electronic Supplementary Material (ESI) for Lab on a Chip. This journal is The Royal Society of Chemistry 2016 Supplementary Information Rapid formation of size-controllable multicellular spheroids via
More informationContinuous-wave biexciton lasing at room temperature using solution-processed quantum wells
CORRECTION NOTICE Continuous-wave bieciton lasing at room temperature using solution-processed quantum wells Joel Q. Grim, Sotirios Christodoulou, Francesco Di Stasio, Roman Krahne, Roberto Cingolani,
More informationLaboratory 3&4: Confocal Microscopy Imaging of Single-Emitter Fluorescence and Hanbury Brown and Twiss setup for Photon Antibunching
Laboratory 3&4: Confocal Microscopy Imaging of Single-Emitter Fluorescence and Hanbury Brown and Twiss setup for Photon Antibunching Jose Alejandro Graniel Institute of Optics University of Rochester,
More informationUnit I - Properties of Matter
Unit I - Properties of Matter Elasticity: Elastic and plastic materials Hooke s law elastic behavior of a material stress - strain diagram factors affecting elasticity. Three moduli of elasticity Poisson
More informationSUPPLEMENTAL MATERIAL I: SEM IMAGE OF PHOTONIC CRYSTAL RESONATOR
1 SUPPLEMENTAL MATERIAL I: SEM IMAGE OF PHOTONIC CRYSTAL RESONATOR Figure S1 below is a scanning electronic microscopy image of a typical evanescently coupled photonic crystal resonator used in these experiments.
More informationMechanical Engineering Ph.D. Preliminary Qualifying Examination Solid Mechanics February 25, 2002
student personal identification (ID) number on each sheet. Do not write your name on any sheet. #1. A homogeneous, isotropic, linear elastic bar has rectangular cross sectional area A, modulus of elasticity
More informationStress analysis of a stepped bar
Stress analysis of a stepped bar Problem Find the stresses induced in the axially loaded stepped bar shown in Figure. The bar has cross-sectional areas of A ) and A ) over the lengths l ) and l ), respectively.
More informationCHAPTER TWO: EXPERIMENTAL AND INSTRUMENTATION TECHNIQUES
CHAPTER TWO: EXPERIMENTAL AND INSTRUMENTATION TECHNIQUES 25 2.1 INSTRUMENTATION The prepared samples were characterized using various techniques. Among which are Dynamic Light Scattering, Zeta Potential
More informationSupporting Information
Supporting Information Wu et al. 1.173/pnas.15492112 SI Text S1) Derivation of the Wind-Chime Model Each pulse generates an angular momentum on each MoS 2 flake that makes them rotate unless they develop
More informationSupplementary Methods
Supplementary Methods Modeling of magnetic field In this study, the magnetic field was generated with N52 grade nickel-plated neodymium block magnets (K&J Magnetics). The residual flux density of the magnets
More informationQuantitative dynamic footprinting microscopy reveals mechanisms of neutrophil rolling
Nature Methods Quantitative dynamic footprinting microscopy reveals mechanisms of neutrophil rolling Prithu Sundd, Edgar Gutierrez, Maria K Pospieszalska, Hong Zhang, Alexander Groisman & Klaus Ley Supplementary
More informationMechanics of Materials II. Chapter III. A review of the fundamental formulation of stress, strain, and deflection
Mechanics of Materials II Chapter III A review of the fundamental formulation of stress, strain, and deflection Outline Introduction Assumtions and limitations Axial loading Torsion of circular shafts
More informationStudy of Rupture Directivity in a Foam Rubber Physical Model
Progress Report Task 1D01 Study of Rupture Directivity in a Foam Rubber Physical Model Rasool Anooshehpoor and James N. Brune University of Nevada, Reno Seismological Laboratory (MS/174) Reno, Nevada 89557-0141
More informationProject PAJ2 Dynamic Performance of Adhesively Bonded Joints. Report No. 3 August Proposed Draft for the Revision of ISO
NPL Report CMMT(A)81 Project PAJ2 Dynamic Performance of Adhesively Bonded Joints Report No. 3 August 1997 Proposed Draft for the Revision of ISO 11003-2 Adhesives - Determination of Shear Behaviour of
More informationDEPARTMENT OF MECHANICAL ENIGINEERING, UNIVERSITY OF ENGINEERING & TECHNOLOGY LAHORE (KSK CAMPUS).
DEPARTMENT OF MECHANICAL ENIGINEERING, UNIVERSITY OF ENGINEERING & TECHNOLOGY LAHORE (KSK CAMPUS). Lab Director: Coordinating Staff: Mr. Muhammad Farooq (Lecturer) Mr. Liaquat Qureshi (Lab Supervisor)
More informationTemperature ( o C)
Viscosity (Pa sec) Supplementary Information 10 8 10 6 10 4 10 2 150 200 250 300 Temperature ( o C) Supplementary Figure 1 Viscosity of fibre components (PC cladding blue; As 2 Se 5 red; CPE black) as
More informationLab 3-4 : Confocal Microscope Imaging of Single-Emitter Fluorescence and Hanbury-Brown and Twiss Set Up, Photon Antibunching
Lab 3-4 : Confocal Microscope Imaging of Single-Emitter Fluorescence and Hanbury-Brown and Twiss Set Up, Photon Antibunching Mongkol Moongweluwan 1 1 Department of Physics and Astronomy, University of
More informationSupplementary information
This journal is The Royal Society of Chemistry 0 Supplementary information Pattern type Area (cm ) Hybrid 0 0 0.00 Hybrid 0 0 0.00 Hybrid 0 0 0.00 Line 00 µm 0.00 Line 0 µm 0.00 Line 0 µm 0.00 Line 0 µm
More informationCollective effects in second-harmonic generation from plasmonic oligomers
Supporting Information Collective effects in second-harmonic generation from plasmonic oligomers Godofredo Bautista,, *, Christoph Dreser,,, Xiaorun Zang, Dieter P. Kern,, Martti Kauranen, and Monika Fleischer,,*
More informationT H E J O U R N A L O F C E L L B I O L O G Y
T H E J O U R N A L O F C E L L B I O L O G Y Supplemental material Eisner et al., http://www.jcb.org/cgi/content/full/jcb.201312066/dc1 Figure S1. Mitochondrial continuity in adult skeletal muscle. (A)
More informationSUPPLEMENTARY INFORMATION
Supplementary Information: Photocurrent generation in semiconducting and metallic carbon nanotubes Maria Barkelid 1*, Val Zwiller 1 1 Kavli Institute of Nanoscience, Delft University of Technology, Delft,
More informationQuantum Optics and Quantum Information Laboratory Review
Quantum Optics and Quantum Information Laboratory Review Fall 2010 University of Rochester Instructor: Dr. Lukishova Joshua S. Geller Outline Lab 1: Entanglement and Bell s Inequalities Lab 2: Single Photon
More informationDmitriy Churin. Designing high power single frequency fiber lasers
Dmitriy Churin Tutorial for: Designing high power single frequency fiber lasers Single frequency lasers with narrow linewidth have long coherence length and this is an essential property for many applications
More informationVirtual Work & Energy Methods. External Energy-Work Transformation
External Energy-Work Transformation Virtual Work Many structural problems are statically determinate (support reactions & internal forces can be found by simple statics) Other methods are required when
More informationModule 4 : Deflection of Structures Lecture 4 : Strain Energy Method
Module 4 : Deflection of Structures Lecture 4 : Strain Energy Method Objectives In this course you will learn the following Deflection by strain energy method. Evaluation of strain energy in member under
More informationMapping of Deformation to Apparent Young s Modulus in Real-Time Deformability Cytometry. Christoph Herold
Mapping of Deformation to Apparent Young s Modulus in Real-Time Deformability Cytometry Christoph Herold ZELLMECHANIK DRESDEN GmbH Tatzberg 47/49 01307 Dresden Germany herold@zellmechanik.com As described
More informationA Portable Optical DSPI System for Residual Stresses Measurement by Hole Drilling Using the Integral Method in Terms of Displacement
A Portable Optical DSPI System for Residual Stresses Measurement by Hole Drilling Using the Integral Method in Terms of Displacement Armando Albertazzi G. Jr. 1, a*, Matias Viotti 1, b, Celso Veiga 1,c
More informationGraphene photodetectors with ultra-broadband and high responsivity at room temperature
SUPPLEMENTARY INFORMATION DOI: 10.1038/NNANO.2014.31 Graphene photodetectors with ultra-broadband and high responsivity at room temperature Chang-Hua Liu 1, You-Chia Chang 2, Ted Norris 1.2* and Zhaohui
More informationNomenclature. Length of the panel between the supports. Width of the panel between the supports/ width of the beam
omenclature a b c f h Length of the panel between the supports Width of the panel between the supports/ width of the beam Sandwich beam/ panel core thickness Thickness of the panel face sheet Sandwich
More informationRaman spectroscopy study of rotated double-layer graphene: misorientation angle dependence of electronic structure
Supplementary Material for Raman spectroscopy study of rotated double-layer graphene: misorientation angle dependence of electronic structure Kwanpyo Kim 1,2,3, Sinisa Coh 1,3, Liang Z. Tan 1,3, William
More informationAbvanced Lab Course. Dynamical-Mechanical Analysis (DMA) of Polymers
Abvanced Lab Course Dynamical-Mechanical Analysis (DMA) of Polymers M211 As od: 9.4.213 Aim: Determination of the mechanical properties of a typical polymer under alternating load in the elastic range
More informationChapter 2 Correlation Force Spectroscopy
Chapter 2 Correlation Force Spectroscopy Correlation Force Spectroscopy: Rationale In principle, the main advantage of correlation force spectroscopy (CFS) over onecantilever atomic force microscopy (AFM)
More informationSNOM Challenges and Solutions
SiO x SiO x Au Au E k SNOM Challenges and Solutions Ralf Vogelgesang, Ph.D. Ralf.Vogelgesang@fkf.mpg.de Nanoscale Science Department (Prof. Kern) Max-Planck-Institut für Festkörperforschung, Stuttgart,
More informationBracing for Earthquake Resistant Design
h z (Draft, 010) Bracing for Earthquae Resistant Design 1 September 18, 00 (010 update) Rigid Roof Idealization and Column Stiffness Relative to the columns, the roof structural system might be quite rigid,
More informationPHYS General Physics II Lab The Balmer Series for Hydrogen Source. c = speed of light = 3 x 10 8 m/s
PHYS 1040 - General Physics II Lab The Balmer Series for Hydrogen Source Purpose: The purpose of this experiment is to analyze the emission of light from a hydrogen source and measure and the wavelengths
More informationEE 5344 Introduction to MEMS CHAPTER 6 Mechanical Sensors. 1. Position Displacement x, θ 2. Velocity, speed Kinematic
I. Mechanical Measurands: 1. Classification of main types: EE 5344 Introduction MEMS CHAPTER 6 Mechanical Sensors 1. Position Displacement x, θ. Velocity, speed Kinematic dx dθ v =, = ω 3. Acceleration
More informationSolution set for EXAM IN TFY4265/FY8906 Biophysical microtechniques
ENGLISH NORWEGIAN UNIVERSITY OF SCIENCE AND TECHNOLOGY DEPARTMENT OF PHYSICS Contact during exam: Magnus Borstad Lilledahl Telefon: 73591873 (office) 92851014 (mobile) Solution set for EXAM IN TFY4265/FY8906
More informationbio-molecular studies Physical methods in Semmelweis University Osváth Szabolcs
Physical methods in bio-molecular studies Osváth Szabolcs Semmelweis University szabolcs.osvath@eok.sote.hu Light emission and absorption spectra Stokes shift is the difference (in wavelength or frequency
More informationEinführung in die Photonik II
Einführung in die Photonik II ab 16.April 2012, Mo 11:00-12:30 Uhr SR 218 Lectures Monday, 11:00 Uhr, room 224 Frank Cichos Molecular Nanophotonics Room 322 Tel.: 97 32571 cichos@physik.uni-leipzig.de
More informationLecture 7: The Beam Element Equations.
4.1 Beam Stiffness. A Beam: A long slender structural component generally subjected to transverse loading that produces significant bending effects as opposed to twisting or axial effects. MECH 40: Finite
More informationSound Propagation through Media. Nachiketa Tiwari Indian Institute of Technology Kanpur
Sound Propagation through Media Nachiketa Tiwari Indian Institute of Technology Kanpur LECTURE-13 WAVE PROPAGATION IN SOLIDS Longitudinal Vibrations In Thin Plates Unlike 3-D solids, thin plates have surfaces
More informationEffect of object-to-camera distance on temperature and spatial resolution of a Thermal imaging system FLIR SC 5000
Effect of object-to-camera distance on temperature and spatial resolution of a Thermal imaging system FLIR SC 5000 B. B. Lahiri, S. Bagavathiappan, John Philip, B.P.C. Rao & T. Jayakumar Non-Destructive
More informationAERO 214. Lab II. Measurement of elastic moduli using bending of beams and torsion of bars
AERO 214 Lab II. Measurement of elastic moduli using bending of beams and torsion of bars BENDING EXPERIMENT Introduction Flexural properties of materials are of interest to engineers in many different
More informationMultiphoton Imaging and Spectroscopy in Cell and Tissue Biophysics. J Moger and C P Winlove
Multiphoton Imaging and Spectroscopy in Cell and Tissue Biophysics J Moger and C P Winlove Relating Structure to Function Biochemistry Raman microspectrometry Surface enhanced Raman spectrometry (SERS)
More informationLab 3 and 4: Single Photon Source
Lab 3 and 4: Single Photon Source By: Justin Deuro, December 10 th, 2009 Abstract We study methods of single photon emission by exciting single colloidal quantum dot (QD) samples. We prepare the single
More informationLow Molecular Weight Gelator Dextran Composites
Low Molecular Weight Gelator Dextran Composites Lin Chen, a Steven Revel, a Kyle Morris, b David G. Spiller, c Louise Serpell, b and Dave J. Adams*,a a Department of Chemistry, University of Liverpool,
More informationSUPPLEMENTARY INFORMATION 1
1 Supplementary information Effect of the viscoelasticity of substrate: In the main text, we indicated the role of the viscoelasticity of substrate. In all problems involving a coupling of a viscous medium
More informationNanoscopy with Focused Light
Nanoscopy with Focused Light Stefan W. Hell Max Planck Institute for Biophysical Chemistry Department of NanoBiophotonics Göttingen & German Cancer Research Center (DKFZ) Optical Nanoscopy Division Heidelberg
More informationINTERNAL STRAIN MEASUREMENTS IN CFRP PLATES SUBJECTED TO IMPACT LOAD USING FBG SENSORS
INTERNAL STRAIN MEASUREMENTS IN CFRP PLATES SUBJECTED TO IMPACT LOAD USING FBG SENSORS J. Frieden, J. Cugnoni, J. Botsis, Th. Gmür, D. Coric Laboratoire de Mécanique appliquée et d Analyse de Fiabilité
More informationD Y N A M I C M E C H A N I C A L A N A L Y S I S A N D I T S A D V A N T A G E S O V E R D E F L E C T I O N T E M P E R A T U R E U N D E R L O A D
D Y N A M I C M E C H A N I C A L A N A L Y S I S A N D I T S A D V A N T A G E S O V E R D E F L E C T I O N T E M P E R A T U R E U N D E R L O A D Sujan E. Bin Wadud TA Instruments 9 Lukens Drive, New
More informationMichelson Interferometer
Michelson Interferometer Objective Determination of the wave length of the light of the helium-neon laser by means of Michelson interferometer subsectionprinciple and Task Light is made to produce interference
More informationMechanical Design in Optical Engineering
Torsion Torsion: Torsion refers to the twisting of a structural member that is loaded by couples (torque) that produce rotation about the member s longitudinal axis. In other words, the member is loaded
More informationHHS Public Access Author manuscript Phys Rev Lett. Author manuscript; available in PMC 2015 January 08.
Grueneisen relaxation photoacoustic microscopy Lidai Wang, Chi Zhang, and Lihong V. Wang * Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis Campus Box
More informationSupporting Information
Electronic Supplementary Material (ESI) for Chemical Communications. This journal is The Royal Society of Chemistry 2014 Supporting Information Near-infrared Light Triggerable Deformation-free Polysaccharide
More informationChapter 5: Random Vibration. ANSYS Mechanical. Dynamics. 5-1 July ANSYS, Inc. Proprietary 2009 ANSYS, Inc. All rights reserved.
Chapter 5: Random Vibration ANSYS Mechanical Dynamics 5-1 July 2009 Analysis Random Vibration Analysis Topics covered: Definition and purpose Overview of Workbench capabilities Procedure 5-2 July 2009
More informationSupplementary Information
Supplementary Information Supplementary Figures Supplementary figure S1: Characterisation of the electron beam intensity profile. (a) A 3D plot of beam intensity (grey value) with position, (b) the beam
More informationMEMS Metrology. Prof. Tianhong Cui ME 8254
MEMS Metrology Prof. Tianhong Cui ME 8254 What is metrology? Metrology It is the science of weights and measures Refers primarily to the measurements of length, weight, time, etc. Mensuration- A branch
More informationUsing the finite element method of structural analysis, determine displacements at nodes 1 and 2.
Question 1 A pin-jointed plane frame, shown in Figure Q1, is fixed to rigid supports at nodes and 4 to prevent their nodal displacements. The frame is loaded at nodes 1 and by a horizontal and a vertical
More informationIntroduction to Nonlinear Optics
Introduction to Nonlinear Optics Prof. Cleber R. Mendonca http://www.fotonica.ifsc.usp.br Outline Linear optics Introduction to nonlinear optics Second order nonlinearities Third order nonlinearities Two-photon
More informationBiomaterial Scaffolds
Biomaterial Scaffolds Biomaterial Properties Surface properties Bulk properties Biological properties Types of Biomaterials Biological materials Synthetic materials Surface Properties The body reads the
More informationDynamic Analysis Contents - 1
Dynamic Analysis Contents - 1 TABLE OF CONTENTS 1 DYNAMIC ANALYSIS 1.1 Overview... 1-1 1.2 Relation to Equivalent-Linear Methods... 1-2 1.2.1 Characteristics of the Equivalent-Linear Method... 1-2 1.2.2
More informationSelf-calibrated, line-scan STED-FCS to quantify lipid dynamics in model and cell membranes
Self-calibrated, line-scan STED-FCS to quantify lipid dynamics in model and cell membranes Aleš Benda, Yuanqing Ma and Katharina Gaus Centre for Vascular Research, Australian Centre for Nanomedicine and
More informationECE280: Nano-Plasmonics and Its Applications. Week8
ECE280: Nano-Plasmonics and Its Applications Week8 Surface Enhanced Raman Scattering (SERS) and Surface Plasmon Amplification by Stimulated Emission of Radiation (SPASER) Raman Scattering Chandrasekhara
More informationMeasurements in Optics for Civil Engineers
Measurements in Optics for Civil Engineers I. FOCAL LENGTH OF LENSES The behavior of simplest optical devices can be described by the method of geometrical optics. For convex or converging and concave
More informationPhysics 208 Exam 1 Oct. 3, 2007
1 Name: Student ID: Section #: Physics 208 Exam 1 Oct. 3, 2007 Print your name and section clearly above. If you do not know your section number, write your TA s name. Your final answer must be placed
More information3D-Printed Fluidic Devices Enable Quantitative Evaluation of Blood Components in Modified Storage Solutions for Use in Transfusion Medicine
Electronic Supplementary Material (ESI) for Analyst. This journal is The Royal Society of Chemistry 214 Supplementary Information 3D-Printed Fluidic Devices Enable Quantitative Evaluation of Blood Components
More informationDynamic Mechanical Analysis of Solid Polymers and Polymer Melts
Polymer Physics 2015 Matilda Larsson Dynamic Mechanical Analysis of Solid Polymers and Polymer Melts Polymer & Materials Chemistry Introduction Two common instruments for dynamic mechanical thermal analysis
More informationSuper-Diffraction Limited Wide Field Imaging and Microfabrication Based on Plasmonics
Super-Diffraction Limited Wide Field Imaging and Microfabrication Based on Plasmonics Peter T. C. So, Yang-Hyo Kim, Euiheon Chung, Wai Teng Tang, Xihua Wang, Erramilli Shyamsunder, Colin J. R. Sheppard
More informationCho Fai Jonathan Lau, Xiaofan Deng, Qingshan Ma, Jianghui Zheng, Jae S. Yun, Martin A.
Supporting Information CsPbIBr 2 Perovskite Solar Cell by Spray Assisted Deposition Cho Fai Jonathan Lau, Xiaofan Deng, Qingshan Ma, Jianghui Zheng, Jae S. Yun, Martin A. Green, Shujuan Huang, Anita W.
More informationAC : ENGINEERING SQUEEZEOMETER AND HUGGOME- TER
AC 202-50: ENGNEERNG SQUEEZEOMETER AND HUGGOME- TER Dr. James Aflaki, Christian Brothers University James Aflaki received his Ph.D. in mechanical engineering from the University of Maryland, College Park.
More information