1. 5 : : O : A. (Aloe), Vol. 7 No. 4 Nov EL ECTROCHEMISTR Y : (2001) , ) 0. 1 mol/ L HAc (p H 2. 89) . 1.

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1 EL ECTROCHEMISTR Y Vol 7 No 4 Nov 2001 : (2001) ,,, (, ) : 0 1 mol/ L HAc (p H 2 89),, I p E p p H V (vs SCE), E p V, I p mol/ L, mol/ L, : ; ; : O : A (Aloe), [1 ], [24 ] [5, ] [6 20 ] [6, ] HPLC [7 ] [8 ] [9 ], 1 5, : , : : (2001J035)

2 XJ P2821 (B) (, ),p HS23B ( ), KQ2250DE ( ) mol/ L : ( ) 6 8 mg, 25 ml, 0 1 mol/ L HAc, 1 2 ( ),,5 # ( GCE),, 2 mmol/ L K 3 Fe (CN) 6, V, 10 ml,0 1 mol/ L HAc,, 5 min, V (vs SCE) 2 min, 10 s, V 0 0 V, V 1 5, V, 100 mv/ s mol/ L HAc 1 5 ( 1), E p = V, I p, 2 2 (1) p H N H 3 N H 4 Cl Na 2 B 4 O 7 NaOH 2 HPO 4 NaH 2 PO 4 KCl HAc NaAc HAc HCl H 2 SO 4 HClO 4,,, HAc, p H mol/ L HAc HAc NaAc p H p H mol L , p H,, ;,, p H 2 89 (2) HAc, HAc 0 05 mol/ L 0 1 mol/ L,

3 4 : , HAc 0 1 mol/ L, HAc, HAc 0 1 mol/ L (3),1 2,,,, Fig th order derivative anodic stripping voltammor V (4) grams of Aloe2emodin 0 1 mol/ L HAc mol/ L HAc mol/ L Aloe2 emodin, 4 h (5) I p mol/ L I p = C I p 1 5 (A), C ( mol/ L ), = mol/ L (6) 5 0g/ 10 ml, I p 5 % K + Na + N H + 4 Ca 2 + Mg 2 + F - Cl - Ac SO 4 NO Zn 2 + Se 2 + Mn 2 + VitB2 VitB6 Cu 2 + Si 4 + 5,4 Fe 3 + NaF Fe 3 +, 50g (7) 6,,,, 4, 10 ml, 100 ml, 2 00 ml,, 1 98 %101 %, 2

4 (6 ) Tab 1 Determination results of Aloe2emodin in sample (n = 6) Sample This Method Spectrophotometry Experimental/ (g/ 100g) 10-3 Mean/ (g/ 100g) S RSD( %) Mean/ (g/ 100g) J uice Skin Gel Whole leaf :S,RSD (3 ) Tab 2 Recovery results of Aloe2emodin in sample (n = 3) Sampl Add/g Found/g Recovery/ % J uice Skin Gel Whole leaf I p E p Tab 3 Effect of the scan rates on the I p and E p v (V/ s) I pa /A E pa / V E pc / V

5 4 : min 0 1 mol/ L HAc mv/ s, I p E p I p, [10 ], V,, E pa E pc ( 3), 2 Fig 2 Cyclic voltammograms of Aloe2emodin in HAc [11, (1) : E p = E 0, + b lg k - b lg v (1) solution 0 1 mol/ L HAc (p H = 2 89) E 0,, b = mol/ L Aloe2emodin mol/ L HAc (p H = 2 89) R T/n F, k 3 E p v, E p lg v, E pa = g v ; E pc = lg v n = 1 24, a = 0 5 [14 ], n = 2 482,= 0 62 ; :n = 0 97, n = 1 952,= p H,HAc NaAc p H,p H , I p p H, E pa E pc p H, E p, p H, 4 E p p H : E pa = p H ; E pc = p H : E pc /p H = ;E pa /p H = ,10, [12 ],I p p H,E p p H, [12 ] ( m ) E p p H,E pc / p H = R T m / n F [13 ], E pc / p H 52 8 mv, m = 2

6 p H Tab 4 Effect of p H on peak current and peak potential of Aloe2emodin p H Peak current Peak potential I pa /A I pc /A - E pa / V - E pc / V % ( ml/ ml) CTMAB, Tween80 OP, SDS 0 5 ml,,, : 4 1),,, 2) HAc, p H Study of Electrochemistry of Aloe2emodin and Determination of It wit h 1 5t h Order Derivative Anodic Stripping Voltammetry L IN Xin2hua, HUAN G Li2ying,CHEN Wei, L UO Hong2bin, Q IU Bin, L I Chun2yan ( Dept of Pharm aceutical, Fujian Medical U niv, Fuz hou , China) Abstract : In 0 1 mol/ L HAc solution(p H = 2 89),the voltammetric behaviours of aloe2emodin

7 4 : at a glassy carbon electode has been studied One pair of current peak of the voltammogram may be att ributed to a quasireversible two2elect ron t ransfer of aloe2emodin molecules at t he glassy car2 bon elect rode A new met hod, t he derivative anodic st ripping voltammet ry, for aloe2emodin de2 termination is described The adsorptive potential of aloe2emodin is V (vs SCE),while it s st ripping potential is V (vs SCE) The peak current is propotional to t he concent ration of aloe2emodin over the range of mol/ L The method is simple, rapid and reliable for aloe2emodin analysis Key words : Aloe2emodin, Voltammet ry,derivative anodic st ripping voltammet ry, Determi2 nation Reference s : [1 ] Ye Yibin, Zhang Xiaohua, Li Han Anthracenc derivatives extracted, separated and identificated from aloe [J ] Journal of Chinese National Folk Medicine,1999,37 :77 [ 2 ] Miguel R B,et al Comparative evaluation of aloe vera in the management of burn wounds in guinea pigs[j ] Plast Reconstr Surg, 1988, 81 (3) :386 [3 ] Davis R H, Leitner M G Topical anti2inflammatory activity of Aloe vera as measured ear swelling[j ] Jour2 nal of the American Pediatric Medical Association,1987,77(11) :610 [ 4 ] Gauntt C J, Wood H J,McDaniel H R,et al Aloepolymannose enhances anti2coxsackievirus antibody titres in mice[j ] Phytother Res, 2000,14(4) :261 [5 ] Yuan Axin, Kang Shuhua, Tan Lin,et al The study on the chemical constituents of aloe vera L var chinesis ( Haw) Berg[J ] Chinese Traditional and Herbal Drugs, 1994,25 (7) ;339 [6 ] Gu Wenxiang, Zu Shuqin Cultivation and Utilization of Aloe [ M ] Shanghai : Shanghai Science Popularit Press, [7 ] Guo Qing, Lu Jin HPLC determination of anthraquinone in radix polygori multiflori andradix polygoni multi2 flori preparata[j ] Journa of Pharmaceutical Analysis,2000,20 (5) :326 [8 ] Zong Yuyin, Yu Mantang, Zhu Zhiqing, et al Micellar electrokinetic capillary chromatography separation and determination of chinese traditional medicine2several rheum species[j ] Acta Pharmaceutica Sinica, 1995,30 (8) :594 [ 9 ] Zhou Jihong, Li Reishi, Liu Zhihong, et al HPTLC determination of three anthraquinone derivatives of Chi2 nese rhubarb in plasma[j ] Journal of Pharmaceutical Analysis, 1995,15(6) :36 [ 10 ] Anson F (translated by Huang Weizeng et al) Electrochemistry and Electro2analytical Chemistry[ M ] Bei2 jin : Peking University Press, [11 ] Laviron E Adsorption, Autoinhibition and Autocatalysis in Polarography and in Linear Potential Sweep Voltammetry[J ] J Electroanal Chem, 1974,52 :355 [ 12 ] Brodbent A D,Sommermann E F The reduction of 9,102anthaquinals Part 2 Polargraphy of 22hydroxy29, 102anthrahydroquinones[J ] J Chem Soc (B), 1968 :519 [13 ] Zou Hong, Yuan Zhoubin Investigation on electrochemical behavior of emodin and its application[j ] Acta Pharmaceutica Sinica, 1997,32(4) :310 [14 ] Gao Xiaoxia Polarographic Catalysis Wave[ M ] Beijin : Science Press,