Reagent ID: 070. General ISE. Test Name: UCSFP. Pre-Dilution Rate: 1 Min OD. Volume R1 Volume. Max OD

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1 URINARY / CSF PROTEIN, AU4/AU4 e System reagent: OSR6x7 Reagent ID: 7 I Operation: Sample: Reagents: Volume R Volume R2 Volume 2 9 Dilution Dilution Dilution Pri. 6 END Sec. 8 + Measuring Point : Measuring inearity: ag Time: Pre-Dilution Rate: Min.. Dynamic : 4* Correlation Factor: A On-board stability period: Max * 9 I rmal s: Age Sex Year ο. ο 2. ο 3. ο 4. ο 5. ο ne Selected 8. Out of Panic Value: evel : Age Year evel : Unit: mg/d* Decimal places: Type Calibration A Formula: Y=AX+ Counts: Process: Point : -Point Cal. Point: M Type Factor: Cal.. With - Factor/- 4* Factor/- 9* Slope Check ne Advanced Calibration: Calibration Period: 9 User defined ot or ot + ottle * Values set for working in mg/d. To work in SI units (g/) divide by ASOSR6x7U

2 URINARY / CSF PROTEIN, AU64/AU64 e System reagent: OSR6x7 Reagent ID: 7 I Operation: Sample: Reagents: Volume R Volume R2 Volume 2 9 Dilution Dilution Dilution Pri. 6 END Sec. 8 + Measuring Point : Measuring inearity: ag Time: Pre-Dilution Rate: Min.. Dynamic : 4* Correlation Factor: A On-board stability period: Max * 9 I rmal s: Age Sex Year ο. ο 2. ο 3. ο 4. ο 5. ο ne Selected 8. Out of Panic Value: evel : Age Year evel : Unit: mg/d* Decimal places: Type Calibration A Formula: Y=AX+ Counts: Process: Point : -Point Cal. Point: M Type Factor: Cal.. With - Factor/- 4* Factor/- 9* Slope Check ne Advanced Calibration: Calibration Period: 9 User defined ot or ot + ottle * Values set for working in mg/d. To work in SI units (g/) divide by ASOSR6x7U

3 URINARY / CSF PROTEIN, AU48 System reagent: OSR6x7 I Sample Volume.6 Dilution Pre-Dilution Rate Reagents Volume: R(R-) R2 (R2-) 52 Dilution Dilution Pri. 6 END nm Sec. 8 nm + Measuring Point : Measuring inearity: ag Time: Operation: imit Min. Max. Reagent ID: 7 ow ow Dynamic ow Correlation Factor A Factor for Maker A Onboard.. 4* 9 Day igh igh igh * our Specific s: evel : From To Sex Year Year ow demographics 8. t within expected values Unit mg/d* Decimal Places evel : igh Panic Value ow igh Calibration Parameters A Formula: Point : Point 8: Point 9: Point: Point Cal. For Master Curve Point : M Type Factor:. of Correction Points User defined * Values set for working in mg/d. To work in SI units (g/) divide by Y=AX+ Counts: Slope Check ne Factor ow igh Allowable Check 4* 9* Reagent lank Calibration Advanced Calibration Operation Interval (R/ACA) ot / ot -Point Calibration Point Type Use Master Curve ot Calibration ow igh Reagent lanks 9 Day our Calibration 9 Day our With - Use Serum Cal. ASOSR6x7U

4 URINARY / CSF PROTEIN, AU68 System reagent: OSR6x7 I Sample Volume.6 Dilution Pre-Dilution Rate Reagents Volume: R(R-) 52 Dilution R2 (R2-) Dilution Common Reagent Type ne Name Pri. 6 END nm Sec. 8 nm + Measuring Point : Measuring inearity: ag Time: Operation: imit Min. Max. Reagent ID: 7 ow ow Dynamic ow Correlation Factor A Factor for Maker A Onboard.. 4* 9 Days igh igh igh * our Specific s: evel : From To Sex Year Year ow demographics 8. t within expected values Unit mg/d* Decimal Places evel : igh Panic Value ow igh Calibration Parameters A Formula: Point : Point 8: Point 9: Point: Point Cal. For Master Curve Point : M Type Factor:. of Correction Points User defined * Values set for working in mg/d. To work in SI units (g/) divide by Y=AX+ Counts: Slope Check ne Factor ow igh Allowable Check 4* 9* Reagent lank Calibration Advanced Calibration Operation Interval (R/ACA) ot / ot -Point Calibration Point Type Use Master Curve ot Calibration ow igh Reagent lanks 9 Day our Calibration 9 Day our With - Use Serum Cal. ASOSR6x7U

5 URINARY / CSF PROTEIN, AU6 System reagent: OSR6x7 Reagent ID: 7 Specific test parameters Test Test name Sample type Page /2 Sample vol. Reagent vol Reagent 2 vol Wave Main Method Reaction Point Fst Point 2 Fst Dil. vol μl Min. Dil. vol μl Dil. vol μl Reagent limit Fst.. Sub 8 st.. END Dynamic range + 4* st Correlation factor st Max. Fst..25 st..25 2* A inearity lag time Fst Sec On-board stability period 9 Select using Space key, or select from list displayed by Guide key Test Test name Sample type Page 2/2 Value/flag rmal range Panic value Sex n select Out of range Age Y M Y M Y M Y M Y M Y M Age Y Y Y Y Y Y evel evel M M M M M M Select the function using the Function key or the Mouse Calibration specificc Test Name Cal type 8 Formula Selection calibrator Cal Point Point 2 Point 3 Point 4 Point 5 Point 6 Point 7 -point cal. point M type factor stability period A Y=AX+ 9 Conc Count Process Factor/- 4* Factor/- 9* Select the function using the Function key or the Mouse To be inserted by the user * Values set for working in mg/d. To work in SI units (g/) divide by ASOSR6x7U

6 URINARY / CSF PROTEIN, AU27/ AU54 System reagent: OSR6x7 Reagent ID: 7 I Operation: Sample: Reagents: Volume R Volume R2 Volume Dilution Dilution Dilution Pri. 6 END Sec. 8 + Measuring Point : Measuring inearity: ag Time: Pre-Dilution Rate: Min.. Dynamic : 4* Correlation Factor: A On-board stability period: Max * 9 I rmal s: Age evel : Sex Year ο. ο 2. ο 3. ο 4. ο 5. ο ne Selected 8. Out of Age Year evel : Panic Value: Unit: mg/d* Decimal places: Type Calibration A Formula: Y=AX+ Counts: Process: Point : -Point Cal. Point: M Type Factor: Cal.. With - Factor/- 4* Factor/- 9* Slope Check ne Advanced Calibration: Calibration Period: 9 User defined ot or ot + ottle * Values set for working in mg/d. To work in SI units (g/) divide by ASOSR6x7U

7 URINARY/CSF PROTEIN, AU58 System Reagent: OSR6x7 Parameters I bac Calculated Test Reagent ID: 7 Operation Sample Volume Pre-Dilution Rate Rgt. Volume R(R-).2 4 R-2 R2(R2-) Common Rgt. Type Wavelength Method Pri ne 6 END nm Reaction Slope Measuring Point st Measuring Point2 st + inearity imit ag Time Check Dilution imit Diluent ottle Min. Max. Dilution Reagent imit Dilution st. ow. igh.25 ow. igh.25 Dilution Dynamic ow 4* igh 2* Name ne Correlation Factor A Sec. 8 nm Factor for Maker A Onboard Period 3 Day our I Influence Check ipemia Icterus emolysis Parameters I bac Calculated Test evel ow igh Specificl s: From To Sex Year Year ow igh ο. ο 2. ο 3. ο 4. ο 5. ο Standardd demographics 8. t within expected values Panic Value ow igh Unit mg/d* Decimal Places Parameters Calibration Parameters s Calibration A Formula: Y= =AX+ Parameters Point : Point 8: Point 9: Point : Point Cal. For Master Curve. of Correction Points r Point- Point-2 M Type Factor: -Point Calibration Point Type Use Serum Cal. User defined. eclman Coulter Urinary/CSF Protein r supplied with kit * Values set for working in mg/d. To work in SI units (g/) divide by ow 45* ow Counts: igh Slope Check * Allowance Check Use Master Curve igh Cuvette. Reagent lank Calibration Advanced Calibration Operation Interval (R/ACA) ot/o ot Calibration Reagent lank 3 Calibration 3 with Conc- Day Day our our ASOSR6x7U

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