Worldwide Recognition for the Synthesis of All 209 PCB Congeners
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- Marilyn Whitehead
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1 Worldwide Recognition for the Synthesis of All 209 PCB Congeners EPA Method 1668 Standards for the Analysis of All 209 Congeners AccuStandard, Inc.
2 Here s why AccuStandard is the recognized market leader in the supply of all 209 PCB congeners... Synthesized all 209 individual PCB congeners in house. Available neat in 5 mg to Bulk quantities requirements. Single component solutions provided in two convenient conc. (35 µg/ml & 100 µg/ml). Offer cost saving Kits containing all 209 congeners (Method 1668 & 9 Solution Dr. Frame RT Study). Purity of 99+% by GC/FID and GC/MS. Identity by m.p., b.p., GC/MS. Cat. # Cross referenced to IUPAC, BZ, and CAS number for easy Congener identification. Over 800 in stock certified PCB congener standards available for immediate delivery. Specialist in Method Specific Congener & Aroclor Applications - Unique formulations for: Toxicity & Abundance Studies Food & Human Tissue Analysis Canadian Methods EPA Methods 680, 8080, 1668, 8082 General Electric Holy Grail Column Retention Time Study Dry Color Manufacturer Association World Health Organization CEN s PCB in Waste Oil ASTM D-2 & D-19 PCB Analysis Many Government Agencies and Companies rely on AccuStandard, including: EPA Center For Disease Control US Dept. of Commerce General Electric NIST NOAA US Army Corps of Engineers Examine our catalog(s) and Web Site for recently Synthesized Compounds & Product lines Hydroxy-Biphenyls Hydroxy-Chlorobiphenyls Bromobiphenyls Bromobiphenyl ethers & other Flame Retardants Chlorodibenzo-p-dioxins Chlorinated Dibenzofurans Haloethers PAH s For a custom PCB congener formulation, For Custom Synthesis, Call , ext. 123 Call , ext. 108 (Technical Service Department) (Custom Synthesis Department) AccuStandard ISO 9001 Certificate Number: Phone: FAX: usa@accustandard.com Toll Free: Toll Free: Web Site:
3 Due to increased domestic and international awareness of the health problems caused by PCB aroclors and congeners the analytical laboratory community needs to determine lower concentrations and identify individual congeners. AccuStandard continues to have an active involvement in the development of standards capable of distinguishing all 209 PCB congeners for these methods. Our latest edition is this listing of certified analytical standards for EPA method 1668 Method 1668 Congener Set 209 Chlorinated Biphenyl Congeners by HRGC / HRMS M-1668A-0.01X-SET $ 700 / 5 x 1 ml (Set includes the following Cat. # s M-1668A X, M-1668A X, M-1668A X, M-1668A X, M-1668A X) Congener specific determination of all 209 chlorinated biphenyl congeners for calibration on a SPB-Octyl capillary column. PCB Congener Mix 1 M-1668A X 83 comps. PCB Congener Mix 2 M-1668A X 54 comps. 3-Chlorobiphenyl 2,6-Dichlorobiphenyl 2,5-Dichlorobiphenyl 2,3'-Dichlorobiphenyl 2,4'-Dichlorobiphenyl 3,5-Dichlorobiphenyl 3,3'-Dichlorobiphenyl 2,4,6-Trichlorobiphenyl 2,3',6-Trichlorobiphenyl 2,4',6-Trichlorobiphenyl 2',3,5-Trichlorobiphenyl 2,3',5-Trichlorobiphenyl 2,4',5-Trichlorobiphenyl 2',3,4-Trichlorobiphenyl 3,3',5-Trichlorobiphenyl 3,4,5-Trichlorobiphenyl 3,3',4-Trichlorobiphenyl 2,2',4,6-Tetrachlorobiphenyl 2,2',3,6-Tetrachlorobiphenyl 2,2',5,5'-Tetrachlorobiphenyl 2,2',4,5'-Tetrachlorobiphenyl 2,4,4',6-Tetrachlorobiphenyl 2,2',3,4-Tetrachlorobiphenyl 2,3',5,5'-Tetrachlorobiphenyl 2,3,3',5-Tetrachlorobiphenyl 2,3,4',5-Tetrachlorobiphenyl 2,3',4,4'-Tetrachlorobiphenyl 3,3',4,5'-Tetrachlorobiphenyl 3,3',4,5-Tetrachlorobiphenyl 3,4,4',5-Tetrachlorobiphenyl 2,2',3,6,6'-Pentachlorobiphenyl 2,2',4,5',6-Pentachlorobiphenyl 2,2',3,5',6-Pentachlorobiphenyl 2,2',3,4,6-Pentachlorobiphenyl 2,2',3,4,6'-Pentachlorobiphenyl 2,2',3,5,5'-Pentachlorobiphenyl 2,3,3',5',6-Pentachlorobiphenyl 2,2',3,3',5-Pentachlorobiphenyl 2,3',4,4',6-Pentachlorobiphenyl 2,2',3,4,5'-Pentachlorobiphenyl 2,2',3,4,4'-Pentachlorobiphenyl 2,2',3,3',4-Pentachlorobiphenyl PCB Congener Mix 3 M-1668A X 3,4'-Dichlorobiphenyl 2,2',4-Trichlorobiphenyl 2,4,5-Trichlorobiphenyl 2,3,3'-Trichlorobiphenyl 2,2',3,6'-Tetrachlorobiphenyl 2,3,5,6-Tetrachlorobiphenyl 2,3,3',6-Tetrachlorobiphenyl 2,2',3,3'-Tetrachlorobiphenyl 2,3',4,5-Tetrachlorobiphenyl 2',3,4,5-Tetrachlorobiphenyl 3,3',5,5'-Tetrachlorobiphenyl 2,2',3,5,6-Pentachlorobiphenyl 2,2',3,3',6-Pentachlorobiphenyl 2,2',4,5,5'-Pentachlorobiphenyl 2,3,3',5,6-Pentachlorobiphenyl 2,3',4,5,5'-Pentachlorobiphenyl 2',3,4,5,5'-Pentachlorobiphenyl 2,3,3',4,5-Pentachlorobiphenyl 2',3,3',4,5-Pentachlorobiphenyl 3,3',4,5,5'-Pentachlorobiphenyl 2,2',3,5,6,6'-Hexachlorobiphenyl 2,2',3,3',6,6'-Hexachlorobiphenyl 2,2',3,4',5,6'-Hexachlorobiphenyl 2,2',3,5,5',6-Hexachlorobiphenyl 2,2',3,4,5',6-Hexachlorobiphenyl 2,2',3,4,5,6'-Hexachlorobiphenyl 2,2',3,4,5,6-Hexachlorobiphenyl 2,2',3,3',5,5'-Hexachlorobiphenyl 2,3,3',4,5',6-Hexachlorobiphenyl 2,2',4,4',5,5'-Hexachlorobiphenyl 2,2',3,3',4,5'-Hexachlorobiphenyl 2,2',3,3',4,5-Hexachlorobiphenyl 2,3,4,4',5,6-Hexachlorobiphenyl 2,3,3',4,5,5'-Hexachlorobiphenyl 2,2',3,3',5,6,6'-Heptachlorobiphenyl 2,2',3,3',4,6,6'-Heptachlorobiphenyl 2,2',3,3',5,5',6-Heptachlorobiphenyl 2,2',3,3',4,5',6-Heptachlorobiphenyl 2,2',3,4,4',5',6-Heptachlorobiphenyl 2,2',3,3',4',5,6-Heptachlorobiphenyl 2,2',3,3',4,4',6-Heptachlorobiphenyl 2,2',3,3',4,5,5'-Heptachlorobiphenyl 2,3,3',4,4',5',6-Heptachlorobiphenyl 2,2',3,3',4,4',5-Heptachlorobiphenyl 2,3,3',4,4',5,6-Heptachlorobiphenyl 2,2',3,3',4,5',6,6'-Octachlorobiphenyl 2,2',3,4,4',5,6,6'-Octachlorobiphenyl 2,2',3,3',4,5,6,6'-Octachlorobiphenyl 2,2',3,3',4,5,5',6-Octachlorobiphenyl 2,2',3,3',4,4',5',6-Octachlorobiphenyl 2,2',3,3',4,4',5,6-Octachlorobiphenyl 2,2',3,3',4,4',5,5'-Octachlorobiphenyl 2,2',3,3',4,4',5,6,6'-Nonachlorobiphenyl 2,2',3,4,5-Pentachlorobiphenyl 2,3,4,5,6-Pentachlorobiphenyl 2,3,3',4',5-Pentachlorobiphenyl 2,2',4,4',5,6'-Hexachlorobiphenyl 2,2',3,4',5,6-Hexachlorobiphenyl 2,2',3,4,4',6'-Hexachlorobiphenyl 2,2',3,4',5,5'-Hexachlorobiphenyl 2,2',3,4,5,5'-Hexachlorobiphenyl 2,3,3',4',5',6-Hexachlorobiphenyl 2,3,3',4,4',6-Hexachlorobiphenyl 2,2',3,4,4',5,6'-Heptachlorobiphenyl 2,2',3,3',4,5,6'-Heptachlorobiphenyl 2,2',3,3',4,5,6-Heptachlorobiphenyl 2,3,3',4',5,5',6-Heptachlorobiphenyl 29 comps. 2,4-Dichlorobiphenyl 2,3-Dichlorobiphenyl 3,4-Dichlorobiphenyl 2,2',5-Trichlorobiphenyl 2,3,6-Trichlorobiphenyl 2,3,5-Trichlorobiphenyl 2,4,4'-Trichlorobiphenyl 2,3,4'-Trichlorobiphenyl 3,4',5-Trichlorobiphenyl 2,2',5,6'-Tetrachlorobiphenyl 2,2',4,6'-Tetrachlorobiphenyl 2,3',5',6-Tetrachlorobiphenyl 2,2',4,5-Tetrachlorobiphenyl 2,3,4,6-Tetrachlorobiphenyl 2,3',4',6-Tetrachlorobiphenyl 2,3',4,5'-Tetrachlorobiphenyl 2,3,3',5'-Tetrachlorobiphenyl 2,3,4,5-Tetrachlorobiphenyl 2,3,3',4-Tetrachlorobiphenyl 2,3,4,4'-Tetrachlorobiphenyl 2,2',3,5,6'-Pentachlorobiphenyl 2,2',4,4',6-Pentachlorobiphenyl 2,2',3,4',6-Pentachlorobiphenyl 2,3',4,5',6-Pentachlorobiphenyl 2,2',3,4',5-Pentachlorobiphenyl 2,2',4,4',5-Pentachlorobiphenyl 2,3,3',4,6-Pentachlorobiphenyl 2,3,4',5,6-Pentachlorobiphenyl 2,3,3',5,5'-Pentachlorobiphenyl 2,3,3',4,5'-Pentachlorobiphenyl 2,2',3,4',6,6'-Hexachlorobiphenyl 2,2',3,4,6,6'-Hexachlorobiphenyl 2,2',3,3',5,6'-Hexachlorobiphenyl 2,2',3,4',5',6-Hexachlorobiphenyl 2,2',3,4,4',6-Hexachlorobiphenyl 2,2',3,3',4,6'-Hexachlorobiphenyl 2,3,3',5,5',6-Hexachlorobiphenyl 2,3',4,4',5',6-Hexachlorobiphenyl 2,2',3,4,4',5-Hexachlorobiphenyl 2,3,3',4,5,6-Hexachlorobiphenyl 2,2',3,3',4,4'-Hexachlorobiphenyl 2,3,3',4',5,5'-Hexachlorobiphenyl 2,2',3,4,4',6,6'-Heptachlorobiphenyl 2,2',3,4,5,6,6'-Heptachlorobiphenyl 2,2',3,4',5,5',6-Heptachlorobiphenyl 2,2',3,4,5,5',6-Heptachlorobiphenyl 2,2',3,4,4',5,6-Heptachlorobiphenyl 2,3,3',4,5,5',6-Heptachlorobiphenyl 2,2',3,3',4,4',6,6'-Octachlorobiphenyl 2,2',3,3',4,5,5',6'-Octachlorobiphenyl 2,2',3,4,4',5,5',6-Octachlorobiphenyl
4 Method 1668 Congener Set (Continued) 209 Chlorinated Biphenyl Congeners by HRGC / HRMS PCB Congener Mix #4 M-1668A X 15 comps. PCB Congener Mix #5 M-1668A X 28 comps. 2,3',4-Trichlorobiphenyl 2,3,4-Trichlorobiphenyl 2,3',4,6-Tetrachlorobiphenyl 2,2',4,4'-Tetrachlorobiphenyl 2,2',3,4'-Tetrachlorobiphenyl 2,3,4',6-Tetrachlorobiphenyl 2,3',4',5-Tetrachlorobiphenyl 2,2',4,5,6'-Pentachlorobiphenyl 2,2',3',4,5-Pentachlorobiphenyl 2,3,4,4',6-Pentachlorobiphenyl 2,2',3,3',5,6-Hexachlorobiphenyl 2,2',3,3',4,6-Hexachlorobiphenyl 2,3,3',4',5,6-Hexachlorobiphenyl 2,2',3,4,4',5,5'-Heptachlorobiphenyl 2-Chlorobiphenyl 2,2'-Dichlorobiphenyl 2,2',6-Trichlorobiphenyl 2,2',3-Trichlorobiphenyl 3,4,4'-Trichlorobiphenyl 2,2',6,6'-Tetrachlorobiphenyl 2,2',3,5-Tetrachlorobiphenyl 2,2',3,5'-Tetrachlorobiphenyl 2,4,4',5-Tetrachlorobiphenyl 2,3,3',4'-Tetrachlorobiphenyl 2,2',4,6,6'-Pentachlorobiphenyl 2,2',3',4,6-Pentachlorobiphenyl 2',3,4,5,6'-Pentachlorobiphenyl 2,3,3',4',6-Pentachlorobiphenyl 2,2',4,4',6,6'-Hexachlorobiphenyl 2,2',3,4,4',5'-Hexachlorobiphenyl 2,2',3,4',5,6,6'-Heptachlorobiphenyl 2,2',3,3',5,5',6,6'-Octachlorobiphenyl 2,3,3',4,4',5,5',6-Octachlorobiphenyl 2,2',3,3',4,5,5',6,6'-Nonachlorobiphenyl 2,2',3,3',4,4',5,5',6-Nonachlorobiphenyl Decachlorobiphenyl Method 1668A Combined Congeners Standard World Health Organization M-1668A-LOC-SET Native PCB Calibration Mix M-1668A-NAT 2,4,4'-Trichlorobiphenyl 2,2',3,3',4,4',5-Heptachlorobiphenyl 2,2',3,4,4',5,5'-Heptachlorobiphenyl 2,2',3,3',4,4',5,5'-Octachlorobiphenyl 2,2',3,3',4,4',5,5',6-Nonachlorobiphenyl 2,2',3,3',4,4',5,5',6,6'-Decachlorobiphenyl $ 150 / 2 x 1 ml (M-1668A-NAT, M-1668A-PAR) Determination of Chlorobiphenyl content at each level of chlorination 19 comps. (1 µg/ml) (20 µg/ml) PAR PCB Spike Mix M-1668A-PAR 19 comps. 2,4,4'-Trichlorobiphenyl (0.2 µg/ml) (1 µg/ml) (2 µg/ml) 2,2',3,3',4,4',5-Heptachlorobiphenyl (2 µg/ml) 2,2',3,4,4',5,5'-Heptachlorobiphenyl (2 µg/ml) 2,2',3,3',4,4',5,5'-Octachlorobiphenyl 2,2',3,3',4,4',5,5',6-Nonachlorobiphenyl 2,2',3,3',4,4',5,5',6,6'-Decachlorobiphenyl (20 µg/ml) Congener Mix C-WHO-01 $ 40 / 2.0 µg/ml each in Isooctane 12 comps. 3,4,4',5-Tetrachlorobiphenyl M-1668A-C-NT-LOC-WD-GCPC $ 150 / M-1668A-C-NT-LOC-WD-GCPC-PAK $ 600 / 5 x 1 ml 20.0 µg/ml each in Isooctane 33 comps. 2-Chlorobiphenyl 2,2'-Dichlorobiphenyl 2,2',6-Trichlorobiphenyl 2,3,5-Trichlorobiphenyl 2',3,5-Trichlorobiphenyl 3,4,4'-Trichlorobiphenyl 2,2',6,6'-Tetrachlorobiphenyl 3,4,4',5-Tetrachlorobiphenyl 2,2',4,6,6'-Pentachlorobiphenyl 2,2',4,4',6,6'-Hexachlorobiphenyl 2,2',3,3',4,4',5-Heptachlorobiphenyl 2,2',3,4,4',5,5'-Heptachlorobiphenyl 2,2',3,4,4',5,6'-Heptachlorobiphenyl 2,2',3,4',5,5',6-Heptachlorobiphenyl 2,2',3,4',5,6,6'-Heptachlorobiphenyl 2,2',3,3',5,5',6,6'-Octachlorobiphenyl 2,3,3',4,4',5,5',6-Octachlorobiphenyl 2,2',3,3',4,4',5,5',6-Nonachlorobiphenyl 2,2',3,3',4,5,5',6,6'-Nonachlorobiphenyl 2,2',3,3',4,4',5,5',6,6'-Decachlorobiphenyl Technical Note M-1668A-C-NT-LOC-WD-GCPC is a combined chlorinated congener standard. This single standard contains the chlorinated congeners selected from the World Health Organization list, the Native Toxics (NT) and Level of Chlorination (LOC) tables along with the required GC/MS Performance Check congeners. The use of this single mix reduces analytical run time (single mix versus 4 separate runs), eliminates standard preparation, and assists in validation of instrument performance criteria. ISO 9001 Certificate Number: Phone: FAX: usa@accustandard.com Toll Free: Toll Free: Web Site:
5 PCB Congener Historical Perspective Polychlorinated biphenyls (PCBs) have been the subject of numerous studies and investigations over the last several decades because of their environmental persistency and bioaccumulation. Their abundance as a pollutant stems from their worldwide manufacture as heavily used industrial chemicals (in the USA, as Aroclors by Monsanto), with the main application as a di-electric fluid for capacitors and transformers. As late as 1984, about 758 million pounds were still in use in the United States alone. Toxicity & BioAccumulation The chemical formulation of PCBs, their physiological properties (liphophilicity causing bioaccumulation in body lipids and liver organs) and overall toxicity are quite similar to that of the banned pesticide, DDT. One of the first signals of the effect of PCBs on the environment, in the United States, was noted in 1970 on Great Gull Island at the entrance to Long Island Sound. Scientists observed a sharp increase in the number of abnormalities found in young sea gulls, such as feather loss, crossed beaks, and four legs. In addition, the egg shells were extremely thin. In 1968, Japan documented the first of over 1200 human patients, many of them children, who developed acneform skin eruptions (chloracne) and other clinical symptoms. These symptoms were eventually traced to the ingestion of the industrial PCB, Kannechlor 400, (trademark of the Kanegafuchi Chemical Industry Company) which had been blended with Rice Oil (Yusho). The effect was thus termed Yusho Disease. The average amount of actual PCBs consumed by the victims was estimated at 2 grams. By 1973, 22 of the 1200 victims had died, 41% from malignant tumors, suggesting a possible excess mortality from that cause. Since then, additional studies have been performed discovering many more occurrences and correlations. PCB Groupings and Formulations Toxicity and Abundance PCB Congeners can be grouped according to their presence in the technical mixtures (i.e. Aroclors) and according to their toxicity, generally falling into the following pattern: ConBrio704 Congeners that contain the fewer chlorine substitutions in the ortho positions are more toxic than those having more chlorines in those positions. The most toxic are the tetra, penta and hexachlorobiphenyl congeners that are unsubstituted in the ortho position. Regulatory Actions The overwhelming problem with PCBs is their continuing environmental impact. Their stability, and widespread prior use, has caused global contamination of soils, rivers and other waterways that could affect our food and water supplies for years to come. This problem has become of paramount concern to the US EPA, which prohibited, under section 6(e) of the Toxic Substances Control Act, the use of PCBs, except in totally enclosed systems, after July 1, 1978; the manufacture of PCBs, after January 1, 1979 and the processing and distribution in commerce of PCBs after July 1, PCBs use as industrial chemicals were totally prohibited after July 1, In order to facilitate environmental monitoring, toxicity studies, and possible destruction of PCBs present in the environment, the EPA permits the synthesis and distribution of small quantities for research purposes. In the course of the investigations, it was determined that some of the 209 congeners that constitute the industrial PCB product behave differently than others and it is very helpful, even essential, to the scientific and regulatory communities, that individual congeners be available. For this reason, the EPA granted manufacturing and export exemptions to a few, select standard manufacturers. The founder of AccuStandard, Inc. was the first to obtain this exemption. AccuStandard is the leader in synthesiz-ing those chemicals and indeed, is the only firm to have made all 209 congeners individually. Our expertise can assist you in your PCB investigations. There are 2 columns that are closest to achieving the holy grail status. (J&W, DB-XLB & SGE s HT 8) which resolve all but 4 pairs of significant congeners and 5 pairs of minor congeners. George Frame and his co-workers at General Electric Company have coordinated a seminal study of specially formulated PCB groups - five of which are composed of the congeners contained in Aroclors, the remaining four mixtures contain those congeners generally absent in Aroclors. AccuStandard prepared and supplied the nine mixtures used in Dr. Frame s study from its inventory of the 209 pure congeners. These nine mixtures were then tested on 17 different columns by independent laboratories and column manufacturers. The resulting chromatographic retention time and response data was compiled. This information has proven invaluable for identification and quantification of the different Aroclors as well as for congener specific analysis. Analytical Chemistry of PCBs Second Edition by Mitchell D. Erickson 1997, 688 pp. This updated and expanded Second Edition of Dr. Erickson s Analytical Chemistry of PCBs appears a decade after the first and is completely revised and updated. This book is a comprehensive review of the analytical chemistry of PCBs. It is part history, part annotated bibliography, part comparison, and part guidance. It is an invaluable resource for chemists. Congener Reference Material AccuStandard s FT/IR, Mass spectral data, melting points and chromatograhic information is included along with chromatographic data from George Frame s study, is available in the Analytical Standards & Reference Material for All 209 PCB Congeners Book. Features: Review of PCB analysis, coverage of standard methods, customer/analyst interactions, overview of PCB background, more than 2,000 bibliographic citations, and background data. PCB-BOOK-001 $165 S-3571 $25
6 AccuStandard AccuStandard, Inc. 125 Market Street New Haven, CT PRSRT STD U.S. POSTAGE PAID NEW HAVEN, CT PERMIT NO. 360 Address Service Requested EPA Method 1668 Standards for the Analysis of All 209 Congeners AccuStandard, the PCB Congeners Experts Cl 1-10
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