GC AND GC/MS. Your Essential Resource for Columns & Supplies

Transcription

1 GC AND GC/MS Your Essential Resource for Columns & Supplies

2 GC AppLICATIonS GC Applications Industry-specific applications from your partner in chromatography With over 0 years of chromatography expertise, Agilent is a great resource for all types of applications. In fact, we re developing new ones every day. Simply turn to the pages listed below for the most current applications based on your area of specialization. Environmental you ll learn how to perform critical analyses such as measuring the levels of atmospheric halocarbons and identifying organochlorine pesticides in soil while meeting your increasing demands for speed and accuracy. Turn to page. Food, Flavors, and Fragrances we ll discuss how to ensure quality, safety, and regulatory compliance for fragrances, perfumes, and essential oils. Applications focus on chiral compounds, menthol, and FAMEs. Turn to page. Industrial Chemicals we ll help you maintain product quality and production efficiency by sharing the latest applications for alcohols, halogenated hydrocarbons, aromatic solvents, phenols, and inorganic gases. Turn to page 0. Life Sciences we ll bring you fully up-to-date on the newest screening methods for controlled substances such as amphetamines, narcotics, and alcohol. We ll also review the latest techniques for monitoring residual solvents. Turn to page. Energy and Fuels here you ll find applications such as the analysis of sulfur compounds in propylene that you can use right away to meet regulatory requirements, improve efficiency, and maintain good environmental stewardship. Turn to page. TIpS & TooLS Search the ScanView database to find almost 000 GC applications and standard methods of all types, old and new. Get your free copy of ScanView at

3 EnVIRonMEnTAL AppLICATIonS, HyDRoCARBonS Environmental Applications, Hydrocarbons Methyl Tert-Butyl Ether (MTBE) FID, Extended 00 Analysis DB-MTBE m x 0. mm,. µm Carrier: Helium at 0 ml/min C for min -00 C at 0 C/min 00 C for min Sampler: Purge and Trap (O.I.A. 0) Trap: Tenax only Preheat: C Desorb: 0 C for min Injection: LVI (Low Volume Injector), 0 C Detector: FID (O.I.A. 0), 00 C Sample: 0 ppb per component in ml water, 0. Methyl-tert-butyl-ether (MTBE). -Methylpentane. -Methylpentane. Diisopropyl ether (DIPE). Ethyl-tert-butyl ether (ETBE). Benzene. tert-amyl methyl ether (TAME). a,a,a-trifluorotoluene. Toluene 0. Ethylbenzene. m-xylene. p-xylene. o-xylene. Cumene.,,-Trimethylbenzene.,,-Trimethylbenzene.,,-Trimethylbenzene. Naphthalene 0 0 GCEH00 Unleaded Gasoline DB-VRX - 0 m x 0. mm,. µm Carrier: Helium at 0 cm/sec (0. ml/min), measured at 0 C 0 C for min 0-00 C at C/min 00 C for min Sampler: Purge and Trap (O.I.A. 0) Trap: BTEX (Supelco) at 0 C during purge Preheat: Desorb: 0 C for min Injection: LVI (Low Volume Injector) Detector: A: FID, 0 C B: PID (O.I.A. 0), 00 C Sample: ppb gasoline in ml water A: FID B: PID. -Methylpentane. Benzene. iso-octane. Toluene. Ethylbenzene. m, p-xylene. o-xylene.,,-trimethylbenzene. Naphthalene GCEH00

4 EnVIRonMEnTAL AppLICATIonS, HyDRoCARBonS Determination of Chlorophenols in water and Soil VF-ms Cp 0 m x 0. mm, 0. µm 0 C, 0 C/min to 00 C Carrier: He 0 kpa, 0. bar,. psi Injection: Splitless, initial time: min; Splitflow: 0 ml/min 0 C µl. Phenol. -chlorophenol. -chlorophenol. -chlorophenol.,-dichlorophenol.,+,-dichlorophenol.,-dichlorophenol.,-dichlorophenol.,-dichlorophenol 0.,,-trichlorophenol.,,-trichlorophenol.,,-trichlorophenol.,,-trichlorophenol.,,-trichlorophenol.,,-trichlorophenol.,,,-tetrachlorophenol.,,,-tetrachlorophenol.,,,-tetrachlorophenol Detector: Sample: Sample Conc: Dr. Weßling, Laboratorien GmbH MS 0 C Isohexane Standard, µg/ml, derivatization with acetic acid anhydride min pbdes by ECD DB-XLB m x 0. mm, 0.0 µm Agilent Technologies custom column Carrier: Hydrogen at cm/sec at 00 C (.0 ml/min), constant flow mode 00 C for 0. min 00 C to 00 C at 0 C/min 00 C for min Injection: Split, 0 C Split ratio 0: Detector: ECD, 00 C Peak, Congener (. mg/ml) Sample: µl Special thanks to AccuStandard, Inc. of New Haven, CT, for PBDE standards..,',-tribde (BDE-).,,'-TriBDE (BDE-).,',',-Tetra-BDE (BDE-).,',,'-Tetra-BDE (BDE-).,',,'-TetraBDE (BDE-).,',,',-PentaBDE (BDE-00).,',,',-PentaBDE (BDE-) 0.,',,,'-PentaBDE (BDE-).,',,',,'-HexaBDE (BDE-) 0.,',,',,'-HexaBDE (BDE-).,',,,','-HexaBDE (BDE-).,',,,',',-HeptaBDE (BDE-).,,',,',,-HeptaBDE (BDE-0). DecaBDE (BDE-0) (. mg/ml) BDE GCES00 0

5 EnVIRonMEnTAL AppLICATIonS, HyDRoCARBonS Diesel Fuel DB-ms - 0 m x 0. mm,.0 µm Carrier: Helium at. cm/sec, measured at 0 C 0 C for min 0-00 C at C/min 00 C for 0 min Injection: Direct, 0 C Detector: FID, 0 C Nitrogen makeup gas at 0 ml/min Sample: µl injection in hexane A - Standard, 0 ng/component B - Sample, 0. mg/ml Diesel Fuel Standard 0 ng/component A Septum: mm Advanced Green septa, - Liner: Direct connect, single taper, deactivated, mm id, G-00 Syringe: 0 µl tapered, FN -s//hp, - Diesel Fuel 0. mg/ml B. n-c 0, Decane. n-c, Dodecane. n-c, Tetradecane. n-c, Hexadecane. n-c, Octadecane. n-c 0, Eicosane. n-c, Docusane. n-c, Tetracosane. n-c, Hexacosane 0. n-c, Octacosane GCEH00 Analysis of polycyclic Aromatic Hydrocarbons VF-Xms Cp0 0 m x 0. mm, 0.0 µm Sample: µl ca. ng per component on-column Carrier: Helium, 0 kpa Injection: Split, T= C Detector: Ion Trap MS 0 +. Naphthalene. Acenaphthylene. Acenaphthene. Fluorene. Phenanthrene. Anthracene. Fluoranthene. Pyrene. Chrysene 0. Benzo(a)anthracene. Benzo(k)fluoranthene. Benzo(b)fluoranthene. Benzo(a)pyrene. Indeno(,,-cd)pyrene. Dibenz(a,h)anthracene. Benzo(g,h,i)perylene min VGC000

6 EnVIRonMEnTAL AppLICATIonS, HyDRoCARBonS Dioxins and Dibenzofurans Cp-Sil Cp 0 m x 0. mm, 0.0 µm Sample: Sample Conc: Carrier: Injection: Detector:.0 µl Toluene pg/µl Helium, 0 kpa (. bar, psi) 00 C to 0 C to 0 C, C/min Splitless MSD 0.,,--TCDD.,,,-TCDF.,,,,-PeCDF.,,,,,-HxCDF.,,,,,-HxCDF.,,,,-PeCDF.,,,,,-HxCDD +,,,,-PeCDD.,,,,,-HxCDD.,,,,,-HxCDD 0.,,,,,,-HxCDF.,,,,,-HpCDD.,,,,,,-HpCDD.,,,,,,,-OCDF.,,,,,,,-OCDD 0 min VGC000 Large Mix ng Column AccuStandard 0 Mixes,,, a, b, & ( compounds) DB-ms Ultra Inert -UI 0 m x 0. mm, 0. µm Injection: Detector: 0 C ( min) to 00 C ( C/min), 0 C/min to 0 C ( min), C/min to 0 C ( min) 0 C, purge flow 0 C ml/min at 0. min, gas saver 0 ml/min on at min MSD; Transfer line 0 C, Source 00 C, Quad 0 C. n-nitrosodimethylamine. -methyl pyridine. Benzidene. Fluoranthene. Benzo (g,h,i) perylene LargeMix

7 EnVIRonMEnTAL AppLICATIonS, HyDRoCARBonS polybrominated Diphenyl Ethers (pbdes) DB-ms Ultra Inert -UI m x 0. mm, 0. µm Instrument: Agilent 0N/B MSD Sampler: Agilent B,.0 µl syringe (P/N -),.0 µl splitless injection, ng each component on-column Carrier: Helium cm/s, constant flow Inlet: Pulsed splitless; C, 0 psi until. min, purge flow 0 ml/min at.0 min 0 to C ( C/min), hold min Detector: MSD source at 00 C, quadropole at 0 C, transfer line at 00 C, scan range amu Abundance Liner: Direct connect, dual taper, deactivated, mm id, G-000 Syringe: Autosampler syringe, 0. µl, g, cone, BDE-. BDE-00. BDE-. BDE-. BDE-. BDE-. BDE-0. BDE-0 Time + EU priority pahs Resolution of Critical pairs on an Agilent J&w DB-EUpAH Column DB-EUpAH - 0 m x 0. mm, 0. µm Instrument: Sampler: Carrier: Inlet: Detector: Agilent 0N/B MSD Agilent B,.0 µl syringe, 0. µl splitless injection, injection speed µl/min Helium, ramped flow.0 ml/min (0. min), ml/min to. ml/min C splitless, purge flow 0 ml/min at 0. min C (0. min) to 00 C ( C/min),. C/min to C, C/min to C, C/min to 00 C, 0 C/min to 0 C (. min) MSD source at 00 C, quadrupole at 0 C, transfer line at 0 C, scan range 0-0 AMU Abundance Septum: mm Advanced Green septa, - Liner: Direct connect, dual taper, deactivated, mm id, G-000 Syringe: µl tapered, FN -s//hp, - Rs. Rs.. Benzo[c]fluorene. Benz[a]anthracene. Cyclopenta[c,d]pyrene. Chrysene. -Methylchrysene. Benzo[b]fluoranthene. Benzo[k]fluoranthene. Benzo[j]fluoranthene Rs. Rs. Rs.. Benz[a]pyrene 0. Indeno[,,-cd]pyrene. Dibenz[a,h]anthracene. Benzo[g,h,i]perylene. Dibenzo[a,l]pyrene. Dibenzo[a,e]pyrene. Dibenzo[a,i]pyrene. Dibenzo[a,h]pyrene Time EU_PAHs All + EU regulated priority PAHs are well resolved with the DB-EUPAH column. Challenging benzo[b,k,j]fluoranthene isomers are baseline resolved, allowing for accurate quantitation of each isomer. In addition, baseline resolution is achieved for critical pairs benz[a]anthracene and cyclopenta[c,d]pyrene, cyclopenta[c,d]pyrene and chrysene, and indeno[,,-cd]pyrene and dibenz[a,h]anthracene. This application demonstrates that the DB-EUPAH column can provide excellent sensitivity and selectivity for the analysis of EU regulated PAHs.

8 EnVIRonMEnTAL AppLICATIonS, pesticides AnD HERBICIDES Environmental Applications, Pesticides and Herbicides Fast CLp pesticides DB-CLp - 0 m x 0. mm, 0. µm DB-CLp - 0 m x 0. mm, 0. µm Instrument: Agilent 0 GC with dual µecd Carrier: Helium, constant flow. ml/min 0 C (hold 0. min), C/min to 0 C, C/min to 00 C, 0 C/min to 0 C, hold. min Sampler: Agilent Injection: µl splitless Detector: 0 C Sample: 0 ng/ml CLP Pesticides 0. Tetrachloro-m-xylene. a-bhc. g-bhc. b-bhc. Heptachlor. d-bhc. Aldrin. Heptachlor epoxide. g-chlordane 0. a-chlordane. Endosulfan I 0., -DDE. Dieldrin. Endrin., -DDD. Endosulfan II., -DDT. Endrin aldehyde. Endosulfan sulfate 0. Methoxychlor. Endrin ketone. Decachlorobiphenyl 0, 0

9 EnVIRonMEnTAL AppLICATIonS, pesticides AnD HERBICIDES organochlorine pesticides, EpA Method 0B DB-CLp - 0 m x 0. mm, 0. µm DB-CLp - 0 m x 0. mm, 0. µm Instrument: Agilent 0 GC with dual µecd Carrier: Helium at, cm/sec (constant flow) 0 C (hold 0. min) to 0 C at 0 C/min, C/min to C, C/min to 00 C, hold min Sampler: Agilent Injection: µl splitless Detector: C Sample: 0 ng/ml 0B analytes ,-Dibromo--chloropropane. Hexachlorocyclopentadiene. -Bromo--nitrobenzene. Etradiazole. Chloroneb. Trifluralin. TCMX. Propachlor. Diallate isomers (0ng/mL) 0. Hexachlorobenzene. a-bhc. Pentachloronitrobenzene. g-bhc. b-bhc. Heptachlor 0 0 0,, ,,. Dichlone. Alachlor. d-bhc. Chlorothalonil 0. Aldrin. DCPA. Isodrin. Kelthane. Heptachlor epoxide. g-chlordane. trans-nonachlor. a-chlordane. Endosulfan I., -DDE 0. Dieldrin. Chlorobenzilate (0ng/mL). Perthane (0ng/mL). Chloropropylate (0ng/mL). Endrin. Nitrofen., -DDD. Endosulfan II., -DDT. Endrin aldehyde 0. Endosulfan sulfate. Captafol. Methoxychlor. Endrin ketone. Mirex. cis-permethrin. trans-permethrin. ΩΩΩΩDecachlorobiphenyl 0 0 DB-UI organic Acid performance DB-Select Ultra Inert -0UI 0 m x 0. mm,. µm Carrier: Hydrogen cm/s 0 C isothermal Inlet: Split, : at 0 C Detector: FID at 0 C A. Formic acid. Acetic acid. Propionic acid. Acrylic acid. Isobutyric acid. n-butyric acid. Methacrylic acid. Isopentanoic acid. n-pentanoic acid 0. n-heptanoic acid. Levulinic acid. -Propyl pentanoic acid. -Ethyl hexanoic acid. Citronellic acid 0 0 R

10 EnVIRonMEnTAL AppLICATIonS, pesticides AnD HERBICIDES US EpA Method. Column A: Hp-ms Ultra Inert 0S-UI 0 m x 0. mm,.00 µm Septum: mm Advanced Green septa, - Column B: DB-0-0 m x 0. mm,.00 µm Liner: Direct connect, dual taper, deactivated, mm id, G-000 Syringe: µl tapered, FN -s//hp, - Instrument: Agilent 0A GC Sampler: Carrier: Inlet: Retention Gap: Detector: Agilent B,.0 µl syringe (Agilent p/n -) 0. µl splitless injection Helium cm/s, constant flow Splitless; 00 C, purge flow 0 ml/min at 0. min m, 0. mm id deactivated fused silica high-temperature tubing (Agilent p/n 0--) C ( min) to 0 C ( C/min), hold min, C/min to C, hold 0 min Dual GA µecd; 00 C, const col + makeup (N ) = 0 ml/min This application successfully demonstrates the use of the HP-ms Ultra Inert column for primary analysis of EPA. chlorinated solvents, trihalomethanes and disinfection by-products. The excellent peak shape of the chloral hydrate and resolution between bromodichloromethane and trichloroethylene emphasize the high column inertness of the HP-ms Ultra Inert column, making it an excellent choice for EPA Method. analysis. Hz Hz Column A Column B. Chloroform.,,-Trichloroethane. Carbon tetrachloride. Trichloroacetonitrile. Dichloroacetonitrile. Bromodichloromethane. Trichloroethylene. Chloral hydrate.,-dichloro--propanone 0.,,-Trichloroethane Chloropicrin. Dibromochloromethane. Bromochloroacetonitrile.,-Dibromoethane. Tetrachloroethlyene.,,-Trichloro--propanone. Bromoform. Dibromoacetonitrile.,,-Trichloropropane 0.,-Dibromo--chloropropane 0 min min EPA_- TIpS & TooLS Learn more about the Agilent 0A GC System at

11 EnVIRonMEnTAL AppLICATIonS, pesticides AnD HERBICIDES Analysis of Semivolatiles Column A: Column B: DB m x 0. mm, 0.0 µm DB m x 0. mm, 0. µm Carrier: He constant-flow mode,. ml/min 0 C ( min), C/min to 0 C.0 min hold Injection: Splitless 0. µl injected at 00 C, QuickSwap pressure.0 psi during acquisition, 0.0 psi during backflush with inlet set to.0 psi during backflush A U.S. EPA Method 0, ng/ml System Performance Check Compounds Chromatogram using a DB-., 0 m x 0. mm, 0. µm B GCHE00 Detector: Agilent C Performance Turbo MSD equipped with mm large-aperture drawout lens, P/N G-00 Translating 0. mm id column method to 0. mm id format results in % reduction in analysis time. Resolution of peaks of interest is also maintained for the faster 0. mm id separation GCHE00 U.S. EPA Method 0, ng/ml System Performance Check Compounds Chromatogram using a DB-., 0 m x 0. mm, 0. µm

12 EnVIRonMEnTAL AppLICATIonS, pesticides AnD HERBICIDES pesticides, EpA 0. Carrier: DB-ms - 0 m x 0. mm, 0. µm DB-XLB - 0 m x 0. mm, 0.0 µm Helium at cm/sec (EPC in constant flow mode) C for 0. min -00 C at 0 C/min 00 C for min Injection: Splitless, 0 C 0 sec purge activation time Detector: µecd, 0 C Nitrogen makeup gas (column + makeup flow = 0 ml/min constant flow) Sample: 0 pg per component DB-ms DB-XLB, 0 0 Septum: mm Advanced Green septa, - Liner: Direct connect, single taper, deactivated, mm id, G-00 Syringe: 0 µl tapered, FN -s//hp, GCEP00,0,, GCEP Hexachlorocyclopentadiene. Etridiazole. Chloroneb. Trifluralin. Propachlor. Hexachlorobezene. a-bhc. Atrazine. Pentachloronitrobenzene 0. Simazine. g-bhc. b-bhc. Heptachlor. Alachlor. d-bhc. Chlorothalonil. Aldrin. Metribuzin. Metolachlor 0. DCPA.,'-Dibromobiphenyl. Heptachlor epoxide. Cyanazine. g-chlordane. a-chlordane. Endosulfan I.,'-DDE. Dieldrin. Chlorobenzilate 0. Endrin.,'-DDD. Endosulfan II.,'-DDT. Endrin aldehyde. Endosulfan sulfate. Methoxychlor. cis-permethrin. trans-permethrin

13 EnVIRonMEnTAL AppLICATIonS, pesticides AnD HERBICIDES phenoxy Acid Herbicides Methyl Derivatives, EpA A DB-ms - 0 m x 0. mm, 0. µm Carrier: Helium at cm/sec (EPC in constant flow mode) 0 C for 0. min 0-00 C at C/min 00-0 C at C/min 0 C for min Injection: Splitless, 0 C 0 sec purge activation time Detector: µecd, 0 C Nitrogen makeup gas (column + makeup flow = 0 ml/min constant flow) Sample: 0 pg per component Septum: mm Advanced Green septa, - Liner: Splitless, single taper, deactivated, mm id, - Syringe: 0 µl tapered, FN -s//hp, -. Dalapon.,-Dichlorobenzoic acid. -Nitrophenol. Methyl-,-dichlorophenylacetate (SS). Dicamba. MCPP. MCPA.,'-Dibromooctafluorobiphenyl (IS). Dichloroprop 0.,-D. Pentachlorophenol.,,-T,P.,,-T. Chloramben. Dinoseb.,-DB. Bentazone. DCPA. Picloram 0. Acifluorofen DB-ms GCEP0

14 EnVIRonMEnTAL AppLICATIonS, pesticides AnD HERBICIDES Direct Comparison for Rapid CLp (Contract Laboratory program) pesticide Analysis DB-ms - 0 m x 0. mm, 0. µm DB-ms primary column DB-XLB confirmatory column Carrier: Injection: Detector: DB-XLB - 0 m x 0. mm, 0. µm Hydrogen ( cm/sec at 0 C, ramped at ml/min to 0 cm/sec at. minutes) 0 C (0. min); 0 C/min to 0 C; 0 C/min to C (0. min);. C/min to 00 C (. min) Split/splitless; 0 C, pulsed splitless ( psi for 0. min, purge flow of 0 ml/min on at minute, gas saver flow 0 ml/min on minutes) µecd 0 C; nitrogen makeup; constant column + makeup flow 0 ml/min Hz Hz Hz Vendor R primary column, 0 m x 0. mm, 0. µm Vendor R confirmatory column, 0 m x 0. mm, 0. µm Co-elution Peak tailing 0 Peak tailing 0 GCHE00. Tetrachloro-m-xylene. a-bhc. g-bhc. b-bhc. d-bhc. Heptachlor. Aldrin. Heptachlor Epoxide. g-chlordane 0. a-chlordane. Endosulfan I.,' DDE. Dieldrin. Endrin.,' DDD. Endosulfan II.,' DDT. Endrin Aldehyde. Endosulfan Sulfate 0. Methoxychlor. Endrin Ketone. Decachlorobiphenyl Hz Peak tailing Peak tailing Baseline rise 00 GCHE00 Agilent's DB-ms primary column and DB-XLB confirmatory column sufficiently resolved all the peaks of interest in less than minutes with sharp, symmetrical peaks and minimal baseline drift. In contrast, vendor R's primary analysis column resolved only 0 of peaks with visible peak tailing. Vendor R's confirmatory column resolved all peaks of interest but with peak tailing and an unacceptable level of temperature dependent baseline drift. 0

15 EnVIRonMEnTAL AppLICATIonS, pesticides AnD HERBICIDES Aroclors 0- (without ) DB-XLB - 0 m x 0. mm, 0. µm Carrier: Helium at cm/sec, measured at 0 C 00 C for min 00- C at. C/min Injection: Hot on-column, 0 C Detector: MSD, 0 C transfer line, SIM Sample: µl in isooctane,. ppm GCES00 Septum: mm Advanced Green septa, - Liner: Direct connect, single taper, deactivated, mm id, G-00 Syringe: 0 µl tapered, FN -s//hp, - CLp pesticides DB-ms - 0 m x 0. mm, 0. µm DB-XLB - 0 m x 0. mm, 0.0 µm Carrier: Helium at cm/sec (EPC in constant flow mode) 0 C for 0. min 0-0 C at C/min 0 C for min Injection: Splitless, 0 C 0 sec purge activation time Detector: µecd, 0 C Nitrogen makeup gas (column + makeup flow = 0 ml/min constant flow) Sample: 0 pg per component DB-ms Septum: mm Advanced Green septa, - Liner: Splitless, single taper, deactivated, mm id, - Syringe: 0 µl tapered, FN -s//hp, DB-XLB Complete resolution and confirmation of CLp pesticides in under minutes! 0 0 GCEP00. Tetrachloro m-xylene (SS). a-bhc. g-bhc. b-bhc. Heptachlor. d-bhc. Aldrin. Heptachlor epoxide. g-chlordane 0. a-chlordane. Endosulfan I.,'-DDE. Dieldrin. Endrin.,'-DDD. Endosulfan II.,'-DDT. Endrin aldehyde. Endosulfan sulfate 0. Methoxychlor. Endrin ketone. Decachlorobiphenyl (SS) 0 SS - Surrogate Standard 0 GCEP00

16 EnVIRonMEnTAL AppLICATIonS, pesticides AnD HERBICIDES High Speed VoC, EpA Method 0 DB-VRX - 0 m x 0. mm,.00 µm Carrier: Helium at cm/sec (. ml/min) C for.0 min -0 C at C/min 0- C at 0 C/min C for 0. min Sampler: Purge and trap (Tekmar 00) Purge: min Trap: Vocarb 000 Preheat: C Desorb: 0 C for min Bake: 0 C for 0 min Line & valve:00 C Injection: Split, 0 C Split ratio 0: Detector: Sample: Agilent MSD Scan range: -0 amu Scan rate:. scans/sec Quad temperature: 0 C Source temperature: 00 C Transfer line temp: 00 C ml Halogentated and aromatic analytes at 0 ppb Internal standards at 0 ppb Polar analytes (i.e., ethers, alcohols and ketones at ppb) Septum: mm Advanced Green septa, - Liner: Direct,. mm id, Seal: Gold plated seal, 0-0,. Dichlorodifluoromethane. Chloromethane. Hydroxypropionitrile. Vinyl chloride. Bromomethane. Chloroethane. Ethanol. Acetonitrile. Acrolein 0. Trichlorofluoromethane. Isopropyl alcohol. Acetone. Ethyl ether.,-dichloroethene. tert-butyl alcohol. Acrylonitrile. Methylene chloride. Allyl chloride. Allyl alcohol 0. -Propanol. Propargyl alcohol. trans-,-dichloroethene. MTBE.,-Dichloroethane. Propionitrile. -Butanone. Diisopropyl ether. cis-,-dichloroethene. Methacrylonitrile 0. Bromochloromethane. Chloroform.,-Dichloropropane. Ethyl acetate. Ethyl-tert-butyl ether. Methyl acrylate. Dibromofluoromethane (IS). Isobutanol. Dichloroethane-d (IS). Pentafluorobenzene 0.,-Dichloroethane.,,-Trichloroethane. -Chlorobutane. Crotonaldehyde. -Chloroethanol.,-Dichloropropene. -Butanol. Carbon tetrachloride. Chloroacetonitrile. Benzene 0. tert-amylmethyl ether. Fluorobenzene (IS). -Pentanone. Dibromomethane.,-Dichloropropane. Trichloroethene. Bromodichloromethane. -Nitropropane.,-Dioxane. Epichlorohydrin 0. Methyl methacrylate. cis-,-dichloropropene. Propiolactone. Bromoacetone. Pyridine. trans-,-dichloropropene.,,-trichloroethane. Toluene-d (IS). Toluene.,-Dichloropropane 0. Paraldehyde. Ethyl methacrylate. Dibromochloromethane. -Chloropropionitrile.,-Dibromoethane. Tetrachloroethene.,,,-Tetrachloroethane. -Chlorohexane. Chlorobenzene. Ethylbenzene 0. Bromoform. m-xylene. p-xylene. trans-dichlorobutene.,-dichloro--propanol Ideal for MSD, 0,, -0, 0 -,,,, 0, 0, , 0 0, ,,, 0,, , Styrene.,,,-Tetrachloroethane. o-xylene.,,-trichlropropane. cis-dichlorobutene 0. -Bromofluorobenzene (IS). Isopropylbenzene. Bromobenzene. Propylbenzene. -Chlorotoluene. -Chlorotoluene.,,-Trimethylbenzene. Pentachloroethane. tert-butylbenzene.,,-trimethylbenzene GCEV sec-butylbenzene 0.,-Dichlorobenzene 0. Benzylchloride 0.,-Dichlorobenzene-d (IS) 0.,-Dichlorobenzene 0. Isopropyltoluene 0.,-Dichlorobenzene 0. Butylbenzene 0.,-Dibromo--chloropropane 0. Hexachloroethane 0. Nitrobenzene.,,-Trichlorobenzene. Naphthalene. Hexachlorobutadiene.,,-Trichlorobenzene

17 EnVIRonMEnTAL AppLICATIonS, pesticides AnD HERBICIDES pbdes DB-XLB - 0 m x 0. mm, 0.0 µm Carrier: Helium at cm/sec at 00 C (. ml/min), constant flow mode 00 C for min; 00 C to 0 C at 0 C/min, 0 C for min Injection: Cool on-column, oven-track mode Detector: Agilent MSD, C transfer line, EI SIM (ions monitored:.,.0,.,., 00., 0.,.,.,.,.,.) Sample: 0. µl Abundance Abundance Abundance Abundance Ion.0 (.0 to.0): PBDE-0.D Tetra-BDE ppb each :00 :00 :00 :00 Ion.0 (.0 to.0): PBDE-0.D 000 Penta-BDE 00 0 ppb each 0 0:00 :00 :00 :00 Ion.0 (.0 to.0): PBDE-0.D :00 :00 :00 : Ion.0 (.0 to.0): PBDE-0.D 0:00 :00 :00 :00 Hexa-BDE 00 ppb each Hepta-BDE 0 ppb each GCES0 For a complete Application Note, visit select "Online Literature" from the Literature Library and type -00EN into the "Keyword" field.

18 EnVIRonMEnTAL AppLICATIonS, pesticides AnD HERBICIDES EpA Volatiles by GC/MS (Split Injector) DB-VRX - 0 m x 0. mm,.0 µm Carrier: Helium at 0 cm/sec, measured at C C for 0 min -0 C at C/min 0 C for min 0- C at C/min C for min Sampler: Purge and trap (O.I.A. 0) Purge: Helium for min at 0 ml/min Trap: Tenax/Silica Gel/Carbosieve Preheat: C Desorb: 0 C for 0. min Injection: Split, 0 C Split flow 0 ml/min Detector: MSD, C transfer line Full scan -0 amu (m/z subtracted) Septum: mm Advanced Green septa, - Liner: Direct,. mm id, Seal: Gold plated seal kit, -,, ,,, 0 0 0, 0, GCEV00. Dichlorodifluoromethane. Chloromethane. Vinyl chloride. Bromomethane. Chloroethane. Trichlorofluoromethane. Diethyl ether.,-dichloroethene. Acetone 0. Iodomethane. Carbon disulfide. Allyl chloride. Methylene chloride. Acrylonitrile. Methyl-tert-butyl ether. trans-,-dichloroethene. Hexane.,-Dichloroethane. -Butanone 0. cis-,-dichloroethene.,-dichloropropane. Propionitrile. Methyl acrylate. Methacrylonitrile. Bromochloromethane. Tetrahydrofuran. Chloroform. Pentafluorobenzene (IS).,,-Trichloroethane 0. -Chlorobutane.,-Dichloropropene. Carbon tetrachloride. Benzene.,-Dichloroethane.,-Dimethylhexane. Fluorobenzene (IS).,-Difluorobenzene (IS). Trichloroethene.,-Dichloropropane 0. Methyl methacrylate. Dibromomethane. Bromodichloromethane. -Nitropropane. Chloroacetonitrile. cis-,-dichloropropene. -Methyl--pentanone.,-Dichloro--propanone. Toluene. trans-,-dichloropropene 0. Ethyl methacrylate.,,-trichloroethane. Tetrachloroethene.,-Dichloropropane. -Hexanone. Dibromochloromethane.,-Dibromoethane. -Chloro--fluorobenzene (IS). Chlorobenzene.,,,-Tetrachloroethane 0. Ethylbenzene. m-xylene. p-xylene. o-xylene. Styrene. Bromoform. Isopropylbenzene. -Bromofluorobenzene (SS).,,,-Tetrachloroethane. Bromobenzene 0.,,-Trichloropropane. trans-,-dichloro--butene. n-propylbenzene. -Chlorotoluene.,,-Trimethylbenzene. -Chlorotoluene. tert-butylbenzene. Pentachloroethane.,,-Trimethylbenzene. sec-butylbenzene 0.,-Dichlorobenzene. p-isopropyltoluene.,-dichlorobenzene. n-butylbenzene.,-dichlorobenzene. Hexachloroethane.,-Dibromo--chloropropane. Nitrobenzene.,,-Trichlorobenzene. Hexachlorobutadiene 0. Naphthalene.,,-Trichlorobenzene

19 EnVIRonMEnTAL AppLICATIonS, pesticides AnD HERBICIDES EpA Method. Carrier: DB-ms - 0 m x 0. mm, 0. µm Helium at cm/sec, measured at C, constant flow mode C for min -0 C at 0 C/min 0 C for min 0-0 C at C/min 0-0 C at C/min 0- C at C/min C for min - Injection: Splitless, 00 C.0 min purge activation time Focus liner Detector: MSD, C transfer line Full scan m/z -0 Septum: mm Advanced Green septa, - Liner: Direct connect, single taper, deactivated, mm id, G-00 Syringe: 0 µl tapered, FN -s//hp, Sample: - Composite mixture of AccuStandard Method. standards (M-.-SV-ASL, M-.-FS-ASL, M-.-CP-ASL, M-.-NP-ASL, M-.-NP-ASL): target compounds at ng/µl, IS/SS at ng/µl Compound RT m/z Compound RT m/z. Isophorone..,-Dimethyl--nitrobenzene (SS).. Dichlorvos. 0. Hexachlorocyclo-pentadiene.. EPTC.. Mevinphos 0.0. Butylate 0. /. Vernolate 0.. Dimethyl phthalate Terrazole (Etridazole) 0. /.,-Dinitrotoluene 0.. Tillam (Pebulate) 0.. Acenaphthylene 0.. Acenaphthene-d0 (IS). Chloroneb.. -Chlorobiphenyl.. Tebuthiuron..,-Dinitrotoluene.. Molinate. 0. Diethyl phthalate.. Fluorene.. Propachlor. 0. Ethoprop.. Cycloate. /. Chlorpropham.0. Trifluralin. 0. a-bhc..,-dichlorobiphenyl. /. Hexachlorobenzene. 0. Gesatamine (Atraton). /. Prometon. /. Atrazine. 00/. Simazine. 0/. b-bhc.. Pentachlorophenol.. Propazine. /. g-bhc.. Terbufos.. Pronamide. 0. Diazinon. /. Phenanthrene-d0 (IS).. Chlorothalonil.. Phenanthrene.. Terbacil.0. Methyl paraoxon.0 0. Disulfoton.0. Anthracene.0. d-bhc.0.,,-trichlorobiphenyl. 0. Metribuzin. Compound RT m/z. Alachlor. 0. Simetryn.. Ametryn. /0. Heptachlor. 00. Prometryne.0 /. Prebane (Terbutryne). /. Bromacil. 0. Di-n-butyl phthalate.0.,',,'-tetrachlorobiphenyl.0 0. Metolachlor.. Dursban (Chlorpyrifos). /. Cyanazine. /. Dacthal (DCPA methyl ester). 0. Aldrin.. Triadimefon.. Diphenamid. /. MGK- (Isomer A). /. MGK- (Isomer B).. Heptachlor epoxide. 0.,',',,-Pentachlorobiphenyl.. Merphos. 0/. g-chlordane.. Tetrachlorvinphos (Stirifos). 0. Butachlor.0 /0. Pyrene-d0 (SS).. Pyrene. 0. a-chlordane. /. Endosulfan I.. trans-nonachlor Fenamiphos. 0/. Napropamide.. Tricyclazole.. p,p'-dde.. DEF. /.,',,',,'-Hexachlorobiphenyl.0 0. Dieldrin.. Carboxin.. Endrin 0. /. Chlorobenzilate Endosulfan II 0.. p,p'-ddd 0. /. Endrin aldehyde.0. Norflurazon.. Benzyl butyl phthalate.. Endosulfan sulfate.. p,p'-ddt. /. Hexazinone.. Bis(-ethylhexyl) adipate.. Triphenylphosphate (SS). / 00. Endrin ketone (breakdown product). / 0.,',,',,',-Heptachlorobiphenyl. / 0. Benz[a]anthracene. 0. Chrysene-d (IS). 0 0.,',,',,',,'-Octachlorobiphenyl.0 0/ 0. Methoxychlor. 0. Chrysene. 0. Bis(-ethylhexyl) phthalate.0 0. Fenarimol.0 0. cis-permethrin. 0. trans-permethrin.0. Benzo[b]fluoranthene.0. Benzo[k]fluoranthene.. Fluridone.. Benzo[a]pyrene.. Perylene-d (SS).. Indeno[,,-c,d]pyrene.. Dibenz[a,h]anthracene.. Benzo[g,h,i]perylene GCES0

20 EnVIRonMEnTAL AppLICATIonS, pesticides AnD HERBICIDES pesticides and Fire Retardants (US EpA ) DB-ms Ultra Inert -UI 0 m x 0. mm, 0. µm Carrier: Helium, cm/sec, constant flow 0 C ( min) to 0 C ( C/min), 0 C/min to 0 C ( min) Injection: Splitless, 0 C, purge flow 0 in, gas saver 0 ml/min min Detector: Transfer line 0 C, source 00 C, quad 0 C Sample: Pesticide/PBDE standards, ng with ng IS/SS on-column 0, -, USEPA 0.,-Dimethyl--nitrobenzene. Acenaphthalene-D0. Dimethoate. Atrazine. Propazine. Anthracene-D0. Vinclozoline. Prometryne. Bromacil 0. Malathion. Thiazopyr. Dursban. Benthiocarb. Parathion. Terbus sulfone. Bioallethrin. Oxychlordane. Fenamiphos. Nitrophen 0. Norflurazone. Kepone. Hexazinone. Triphenyl phosphate. Bifenthrin. Chrysene-D. BDE-. Mirex. BDE-00. BDE- 0. Perylene-D. Fenvalerate. Esfenvalerate. Hexabromobiphenyl. BDE-

21 EnVIRonMEnTAL AppLICATIonS, pesticides AnD HERBICIDES organochlorine pesticides I EpA Method 0A DB-ms - 0 m x 0. mm, 0. µm Carrier: Helium at cm/sec, measured at 0 C 0 C for min 0-00 C at C/min C at C/min 00 C for min Injection: Splitless, 0 C 0 sec purge activation time Detector: MSD, 00 C transfer line Full scan at m/z 0-00 Sample: µl of µg/ml composite 0A standards, AccuStandard Inc., 0, Septum: mm Advanced Green septa, - Liner: Splitless, single taper, deactivated, mm id, - Syringe: 0 µl tapered, FN -s//hp, - 0.,-Dibromo--chloropropane. -Chloro--nitrobenzotrifluoride (SS). Hexachloropentadiene. -Bromo--nitrobenzene (IS). Terrazole. Chloroneb. Trifluralin. -Bromobiphenyl (SS). Tetrachloro m-xylene (SS) 0. a, a-dibromo-m-xylene. Propachlor. Di-allate A. Di-allate B. Hexachlorobenzene. a-bhc. Pentachloronitrobenzene (IS). g-bhc. b-bhc. Heptachlor 0. Alachlor. d-bhc. Chlorothalonil. Aldrin. Dacthal. Isodrin. Kelthane. Heptachlor epoxide. g-chlordane. trans-nonachlor 0. a-chlordane. Endosulfan I. Captan. p,p'-dde. Dieldrin. Chlorobenzilate. Perthane. Chloropropylate. Endrin. p,p'-ddd 0. Endosulfan II. p,p'-ddt. Endrin aldehyde. Endosulfan sulfate. Dibutylchlorendate (SS). Captafol. Methoxychlor. Endrin ketone. Mirex. cis-permethrin 0. trans-permethrin * Breakdown Products SS - Surrogate Standard IS - Internal Standard GCEP00 Standards used were a composite of individual solutions supplied courtesy of AccuStandard Inc., Science Park, New Haven, CT 0,

22 EnVIRonMEnTAL AppLICATIonS, pesticides AnD HERBICIDES Chlorinated pesticides, EpA Method 0 Carrier: Injection: Hp-ms 0S- 0 m x 0. mm, 0. µm Helium, psi, cm/sec (0 C) constant flow 0 C for min 0-0 C at 0 C/min 0-0 C at C/min 0 C for min 0-0 C at C/min 0 C for min Splitless min purge delay Detector: ECD, 0 C Makeup gas Nitrogen, 0 ml/min Anode purge ml/min Sample: µl Septum: mm Advanced Green septa, - Liner: Direct connect, single taper, deactivated, mm id, G-00 Syringe: 0 µl tapered, FN -s//hp, - 0. Etridiazole. Chloroneb. Propachlor. Trifluralin. a-bhc. Hexachlorobezene. b-bhc. d-bhc. g-bhc 0. Chlorothalonil. Heptachlor Aldrin. DCPA. Heptachlor epoxide. g-chlordane. Endosulfan I. a-chlordane. Dieldrin.,'-DDE 0. Endrin. Endosulfan II. Chlorobenzilate.,'-DDD. Endrin aldehyde. Endosulfan sulfate.,'-ddt. Methoxychlor. cis-permethrin. trans-permethrin GCEP00 organohalide pesticides in water, EpA Method 0 Hp-ms 0S- 0 m x 0. mm, 0. µm Carrier: Helium at.0 ml/min, constant flow, cm/sec (. psi at 0 C) 0 C for min 0- C at 0 C/min C for min - C at C/min C for min -0 C at C/min 0 C for min Injection: Splitless, 0 C min purge delay Septum: mm Advanced Green septa, - Detector: ECD, 00 C Makeup gas: N, 0 ml/min Anode purge ml/min Sample: µl injection volume components EPA-0 targeted pesticides and ppb Aroclor 0 in hexane. Concentration of pesticides: 0 ppb each except. ppm for atrazine and simazine.. Hexachlorocyclopentadiene. Hexachlorobenzene. Simazine. Atrazine. Lindane. Heptachlor. Alachlor. Adrin 0. Heptachlor epoxide 0. d-chlordane. a-chlordane. trans-nonachlor. Dieldrin. Endrin. cis-nonachlor. Methoxychlor Liner: Direct connect, single taper, deactivated, mm id, G-00 Syringe: 0 µl tapered, FN -s//hp, GCEP00

23 EnVIRonMEnTAL AppLICATIonS, pesticides AnD HERBICIDES organochlorine pesticides I DB m x 0. mm, 0.0 µm Carrier: Helium at 0 cm/sec (.0 ml/min) 0- C at C/min C for 0 min Injection: Splitless, 0 C Detector: ECD, 00 C Nitrogen makeup gas at 0 ml/min.,,,-tetrachloro-m-xylene (IS). a-bhc. b-bhc. g-bhc. d-bhc. Heptachlor. Aldrin. Heptachlor epoxide. g-chlordane 0. Endosulfan I. a-chlordane. Dieldrin. p,p -DDE. Endrin. Endosulfan II. p,p -DDD. Endrin aldehyde. Endosulfan sulfate. p,p -DDT 0. Endrin ketone. Methoxychlor. Decachlorobiphenyl (IS) Sample: 0. µl of 00 pg/µl standard in isooctane, Liner: Splitless, single taper, deactivated, mm id, - Septum: mm Advanced Green septa, - Syringe: 0 µl tapered, FN -s//hp, GCEP00 organochlorine pesticides II DB-0-0 m x 0. mm, 0.0 µm Carrier: Helium at 0 cm/sec (.0 ml/min) 0- C at C/min C for 0 min Injection: Splitless, 0 C Detector: ECD, 00 C Nitrogen makeup gas at 0 ml/min Sample: 0. µl of 00 pg/µl standard in isooctane.,,,-tetrachloro-m-xylene (IS). a-bhc. b-bhc. g-bhc. d-bhc. Heptachlor. Aldrin. Heptachlor epoxide. g-chlordane 0. Endosulfan I. a-chlordane. Dieldrin. p,p -DDE. Endrin. Endosulfan II. p,p -DDD. Endrin aldehyde. Endosulfan sulfate. p,p -DDT 0. Endrin ketone. Methoxychlor. Decachlorobiphenyl (IS) Liner: Splitless, single taper, deactivated, mm id, - Seal: mm Advanced Green septa, - Syringe: 0 µl tapered, FN -s//hp, GCEP00

24 EnVIRonMEnTAL AppLICATIonS, pesticides AnD HERBICIDES organochlorine pesticides III DB m x 0. mm, 0.0 µm Carrier: Helium at 0 cm/sec (.0 ml/min) 0- C at C/min C for 0 min Injection: Splitless, 0 C Detector: ECD, 00 C Nitrogen makeup gas at 0 ml/min Sample: 0. µl of 00 pg/µl standard in isooctane.,,,-tetrachloro-m-xylene (IS). a-bhc. b-bhc. g-bhc. d-bhc. Heptachlor. Aldrin. Heptachlor epoxide. g-chlordane 0. Endosulfan I. a-chlordane. Dieldrin. p,p -DDE. Endrin. Endosulfan II. p,p -DDD. Endrin aldehyde. Endosulfan sulfate. p,p -DDT 0. Endrin ketone. Methoxychlor. Decachlorobiphenyl (IS) Septum: mm Advanced Green septa, - Liner: Splitless, single taper, deactivated, mm id, - Syringe: 0 µl tapered, FN -s//hp, GCEP0 organochlorine pesticides IV DB- - 0 m x 0. mm, 0.0 µm Carrier: Helium at 0 cm/sec (.0 ml/min) 0- C at C/min C for 0 min Injection: Splitless, 0 C Detector: ECD, 00 C Nitrogen makeup gas at 0 ml/min Sample: 0. µl of 00 pg/µl standard in isooctane.,,,-tetrachloro-m-xylene (IS). a-bhc. b-bhc. g-bhc. d-bhc. Heptachlor. Aldrin. Heptachlor epoxide. g-chlordane 0. Endosulfan I. a-chlordane. Dieldrin. p,p -DDE. Endrin. Endosulfan II. p,p -DDD. Endrin aldehyde. Endosulfan sulfate. p,p -DDT 0. Endrin ketone. Methoxychlor. Decachlorobiphenyl (IS) Septum: mm Advanced Green septa, - Liner: Splitless, single taper, deactivated, mm id, - Syringe: 0 µl tapered, FN -s//hp, - 0, GCEP0 0

25 EnVIRonMEnTAL AppLICATIonS, pesticides AnD HERBICIDES organochlorine pesticides, DB-/DB-0p DB m x 0. mm, 0. µm DB-0p - 0 m x 0. mm, 0. µm Guard Column m x 0. mm, 0. µm Carrier: Helium at. cm/sec, measured at 0 C 0 C for 0. min 0-0 C at 0 C/min 0-0 C at C/min 0 C for min Injection: Splitless, 00 C Detector: ECD, C Nitrogen makeup gas at 0 ml/min Sample:.0 µl, 0-00 pg/µl. Etridiazole. Chloroneb. Propachlor. Tetrachloro-m-xylene (IS). Trifluralin. a-bhc. Hexachlorobenzene. b-bhc. g-bhc 0. Pentachloronitrobenzene. p,p'-dichlorobiphenyl. d-bhc. Heptachlor. Alachlor. Aldrin. Chlorpyrifos. DCPA. Isodrin. Heptachlor epoxide 0. Captan. g-chlordane. o,p -DDE. Endosulfan I. a-chlordane. Dieldrin. p,p -DDE. o,p -DDD. Endrin. Endosulfan II 0. Chlorobenzilate. p,p -DDD. o,p -DDT. Endrin aldehyde. Endrin ketone. Carbophenothion. p,p -DDT. Endosulfan sulfate. Hexabromobenzene (HBB). Methoxychlor 0. Mirex. cis-permethrin. trans-permethrin. Decachlorobiphenyl (IS) Enlarged view of critical pair on DB DB DB-0P, 0, 0, 0 Enlarged view of critical pair on DB-0P

26 EnVIRonMEnTAL AppLICATIonS, pesticides AnD HERBICIDES organochlorine pesticides VF-ms Cp 0 m x 0. mm, 0. µm Sample:.0 µl Sample Conc: 00 µg/ml Carrier: Helium, 0 kpa Injection: Splitter, :00 Detector: MS, Ion Trap, TIC 00 Percent 0 0. a-bhc. b-bhc. d-bhc. g-bhc (lindane). Heptachlor. Aldrin. Heptachlorepoxide. Endosulfan I., -DDE 0. Dieldrin. Endrin., -DDD. Endosulfan II. Endrin aldehyde., -DDT. Endosulfan sulfate min VGC000 nitrogen/phosphorus Containing pesticides, EpA Method 0 Hp-ms 0S- 0 m x 0. mm, 0. µm Carrier: Helium, 0 cm/sec (. psi) pressure program 0- C at 0 C/min C for min -0 C at C/min 0-0 C at 0 C/min 0 C for min Injection: Splitless, 0 C min purge delay Detector: NPD, 0 C Helium makeup gas at 0 ml/min 0 0, 0 Septum: mm Advanced Green septa, - Liner: Direct connect, single taper, deactivated, mm id, G-00 Syringe: 0 µl tapered, FN -s//hp, - 0. Dichlorvos. EPTC. Butylate. Mevinphos. Vernolate. Pebulate. Tebuthiuron. Molinate. Ethoprop 0. Cycloate. Chlorpropham. Atraton. Simazine. Prometon. Atrazine. Propazine. Terbufos. Pronamide. Diazinon 0. Disulfoton. Terbacil. Metribuzin. Simetryn. Alachlor. Ametryn. Prometryn. Terbutryn. Bromacil. Metolachlor 0. Triademefon. MGK-. Diphenamid. Stirofos. Butachlor. Fenamiphos. Napropamide. Tricyclazole. Merphos. Carboxin 0. Norflurazon. Hexazinone. Fenarimol. Fluridone GCEP0

27 EnVIRonMEnTAL AppLICATIonS, pesticides AnD HERBICIDES Herbicides I DB-XLB - 0 m x 0. mm, 0. µm Septum: mm Advanced Green septa, - Carrier: Helium at cm/sec, measured at 0 C Liner: Splitless, single taper, deactivated, mm id, - 0 C for min 0-0 C at 0 C/min 0-0 C at C/min 0-0 C at 0 C/min 0 C for min Injection: Splitless, 0 C 0 sec purge activation time Detector: MSD, 00 C transfer line Full scan 0-00 Sample: µl x 0-0 ng/µl solution in acetone Syringe: 0 µl tapered, FN -s//hp, -,, 0 0 -,, 0. Monuron. Diuron. EPTC. Dichlobenil. Vernolate. Pebulate. Molinate. Sulfallate. Atraton 0. Prometon. Atrazine. Propazine. Simazine. Terbuthylazine. Pronamide. Secbumeton. Terbacil. Alachlor. Propanil 0. Ametryn. Prometryne. Simetryn. Metribuzin. Terbutryn. Metolachlor. Bromacil. Dacthal. Diphenamid. Butachlor 0. Napropamide. Carboxin. Tricyclazole. Norflurazon. Hexazinone. Difolotan. Fluridone * Impurity GCEP0 Herbicides II DB m x 0. mm, 0. µm Carrier: Helium at cm/sec 0- C at C/min Injection: Split :0, µl Detector: ECD, 00 C Nitrogen makeup gas at 0 ml/min 0. Phorate. Ethoprop. Terbufos. Atrazine. Fonofos. Propachlor. Chlorpyrifos. Alachlor. Metolachlor 0. Cyanazine min GCHERB0

28 EnVIRonMEnTAL AppLICATIonS, pesticides AnD HERBICIDES C and C Halocarbons (Freons) GS-Gaspro - 0 m x 0. mm Carrier: Helium at cm/sec, constant velocity 0 C for min, 0-0 C at 0 C/min 0 C for min 0-00 C at 0 C/min Injection: Splitless, 0 C 0.0 min purge activation time Detector: MSD, 0 C, full scan -0 amu Sample:.0 µl of 00 ppm mixture of AccuStandard M-REF & M-REF-X in methanol Freon #. Chlorotrifluoromethane*. Trifluoromethane. Bromotrifluoromethane B. Chloropentafluoroethane. Pentafluoroethane.,,-Trifluoroethane a. Dichlorodifluoromethane. Chlorodifluoromethane.,,,-Tetrafluoroethane a 0. Chloromethane 0.,,,-Tetrafluoroethane. Bromochlorodifluoromethane B 0 Freon #.,-Difluoroethane a.,-dichloro-,,,-tetrafluoroethane. -Chloro-,,,-tetrafluoroethane. -Chloro-,-difluoroethane b. Dichlorofluoromethane. Trichlorofluoromethane. Chloroethane 0 0. Dichloromethane 0.,-Dichloro--fluoroethane b.,-dichloro-,,-trifluoroethane.,,-trichloro-,,-trifluoroethane.,-dibromo-,,,-tetrafluoroethane B *Peak not shown Septum: mm Advanced Green septa, - Liner: Splitless, single taper, deactivated, mm id, - Seal: Gold plated seal, 0-0 Syringe: 0 µl tapered, FN -s//hp, GCEA00 nitrogen Containing Herbicides (EpA Method 0) DB- - 0 m x 0. mm, 0.0 µm Carrier: Helium at cm/sec ( ml/min), measured at 0 C 0 C for min 0-0 C at C/min 0 C for min Injection: Megabore Direct, 0 C, µl of ng/µl standard Detector: NPD, 0 C 0, 0. Eptam. Sutan. Vernam. Tillam. Ordram. Treflan. Balan. Ro-Neet. Propachlor 0. Tolban. Propazine. Atrazine. Simazine. Terbacil. Sencor. Dual. Paarlan. Prowl. Bromacil 0. Oxadiazon. GOAL. Hexazinone 0 GCNITR0

29 EnVIRonMEnTAL AppLICATIonS, pesticides AnD HERBICIDES Free phenols Hp-0+ 0L- 0 m x 0. mm, 0. µm Carrier: Hydrogen, constant flow cm/sec Injection: Split 00: Detector: FID 00 C 00 ºC min 0 ºC min ºC/min 0. Phenol. -chlorophenol.,-dimethylphenol. -nitrophenol.,-dichlorophenol. -chloro--methylphenol.,,-trichlorophenol.,-dinitrophenol. -nitrophenol 0. -methyl-,-dinitrophenol. Pentachlorophenol 0 FREEPHENS EpA Halogenated pesticides on "0" Type phases VF-0 pesticides Cp00 0 m x 0. mm, 0. µm 0 C, C/min to C Injection: Split, T= C ECD: T= C, pg Highest response for p,p'-ddt Best peak shape for endrin aldehyde FactorFour VF-0ms Tailing on endrin aldehyde Loss of p,p'-ddt Vendor X '0' Tailing on endrin aldehyde Vendor y '0' Degredation of p,p'-ddt VGC000

30 EnVIRonMEnTAL AppLICATIonS, pesticides AnD HERBICIDES organochlorine pesticides to EpA via GC/MS VF-ms Cp 0 m x 0. mm, 0. µm Carrier: Helium, approx..0 ml/min, 0 kpa C + 0 C/min to C Injection: Split/splitless, in split mode, :00 Detector: Ion Trap MS min VGC000. a-bhc. b-bhc. d-bhc. g-bhc (lindane). Heptachlor. Aldrin. Heptachlorepoxide. Endosulfan I., -DDE 0. Dieldrin. Endrin., -DDD. Endosulfan II. Endrin aldehyde., -DDT. Endosulfan sulfate organochlorine pesticides I EpA Method 0A DB-ms - 0 m x 0. mm, 0. µm Carrier: Helium at cm/sec, measured at 0 C 0 C for min 0-00 C at C/min C at C/min 00 C for min Injection: Splitless, 0 C 0 sec purge activation time Detector: MSD, 00 C transfer line Full scan at m/z 0-00 Sample: µl of µg/ml composite 0A standards, AccuStandard Inc. Septum: mm Advanced Green septa, - Liner: Splitless, single taper, deactivated, mm id, - Syringe: 0 µl tapered, FN -s//hp, - Standards used were a composite of individual solutions supplied courtesy of AccuStandard Inc., Science Park, New Haven, CT 0, ,-Dibromo--chloropropane. -Chloro--nitrobenzotrifluoride (SS). Hexachloropentadiene. -Bromo--nitrobenzene (IS). Terrazole. Chloroneb. Trifluralin. -Bromobiphenyl (SS). Tetrachloro m-xylene (SS) 0. a, a-dibromo-m-xylene. Propachlor. Di-allate A. Di-allate B. Hexachlorobenzene. a-bhc. Pentachloronitrobenzene (IS). g-bhc. b-bhc. Heptachlor 0. Alachlor. d-bhc. Chlorothalonil. Aldrin. Dacthal. Isodrin, 0,. Kelthane. Heptachlor epoxide. g-chlordane. trans-nonachlor 0. a-chlordane. Endosulfan I. Captan. p,p'-dde. Dieldrin. Chlorobenzilate. Perthane. Chloropropylate. Endrin. p,p'-ddd 0. Endosulfan II. p,p'-ddt. Endrin aldehyde. Endosulfan sulfate. Dibutylchlorendate (SS). Captafol. Methoxychlor. Endrin ketone. Mirex. cis-permethrin 0. trans-permethrin * Breakdown Products SS - Surrogate Standard IS - Internal Standard GCEP00

31 EnVIRonMEnTAL AppLICATIonS, pesticides AnD HERBICIDES organochlorine pesticides II EpA Method 0A DB-ms - 0 m x 0. mm, 0. µm Carrier: Helium at cm/sec, measured at 0 C 0 C for min 0-00 C at C/min C at C/min 00 C for min Injection: Splitless, 0 C 0 sec purge activation time Detector: MSD, 00 C transfer line Full scan at m/z 0-00 Sample: µl of µg/ml composite 0A standards, AccuStandard Inc. Standards used were a composite of individual solutions supplied courtesy of AccuStandard Inc., Science Park, New Haven, CT 0, ,-Dibromo--chloropropane. -Chloro--nitrobenzotrifluoride (SS). Hexachloropentadiene. -Bromo--nitrobenzene (IS). Terrazole. Chloroneb. Trifluralin. -Bromobiphenyl (SS). Tetrachloro m-xylene (SS) 0. a, a-dibromo-m-xylene. Propachlor. Di-allate A. Di-allate B. Hexachlorobenzene. a-bhc. Pentachloronitrobenzene (IS). g-bhc. b-bhc. Heptachlor 0. Alachlor. d-bhc. Chlorothalonil. Aldrin. Dacthal. Isodrin,. Kelthane. Heptachlor epoxide. g-chlordane. trans-nonachlor 0. a-chlordane. Endosulfan I. Captan. p,p'-dde. Dieldrin. Chlorobenzilate. Perthane. Chloropropylate. Endrin. p,p'-ddd 0. Endosulfan II. p,p'-ddt. Endrin aldehyde. Endosulfan sulfate. Dibutylchlorendate (SS). Captafol. Methoxychlor. Endrin ketone. Mirex. cis-permethrin 0. trans-permethrin * Breakdown Products SS - Surrogate Standard IS - Internal Standard, 0, GCEP00

32 EnVIRonMEnTAL AppLICATIonS, SEMIVoLATILES Environmental Applications, Semivolatiles Agilent's Ultra Inert Test probe Mixture Carrier: Sampler: Injection: Detector: DB-ms Ultra Inert -UI 0 m x 0. mm, 0. µm Hydrogen, constant pressure, cm/s C isothermal Agilent B, 0. µl syringe (P/N -), 0.0 µl split injection Split/splitless; 0 C,. ml/min; split column flow 00 ml/min; gas saver flow ml/min at.0 min FID at C; 0 ml/min air, 0 ml/min hydrogen, ml/min nitrogen makeup A properly deactivated DB-ms Ultra Inert column delivers symmetrical peak shapes, along with increased peak heights, which allow for accurate integration and detection of trace analytes. pa Propionic acid. -Octene. n-octane. -Picoline. n-nonane. Trimethyl phosphate.,-pentanediol. n-propylbenzene. -Heptanol 0. -Octanone. n-decane 0 0 DBmsPeaks Trace Level polycyclic Aromatic Hydrocarbon (pah) Analyses DB-ms Ultra Inert -UI 0 m x 0. mm, 0. µm Carrier: Injection: Helium constant flow 0 cm/s 0 C ( min) to 00 C ( C/min) 0 C to 0 C ( min) C/min. to 0 C ( min) Split/splitless; 0 C,. ml/min. total flow, purge flow 0 ml/min. on at 0. min., gas saver flow 0 ml/min. on at.0 min. Detector: MSD source at 00 C Quadrapole at 0 C Transfer line at 0 C Scan range 0-0 AMU Abundance Naphthalene. Acenaphthylene. Acenaphthene. Fluorene. Phenanthrene. Anthracene. Fluoranthene. Pyrene. Benz[a]anthracene 0. Chrysene. Benzo[b]fluoranthene. Benzo[k]fluoranthene. Benzo[a]pyrene. Indeno[,,-cd]pyrene. Dibenz[a,h]anthracene. Benzo[g,h,i]perylene GCSV_PAH

33 EnVIRonMEnTAL AppLICATIonS, SEMIVoLATILES Tetrachlorodibenzo-p-furans Column A: DB- - 0 m x 0. mm, 0. µm Column B: DB-ms - 0 m x 0. mm, 0. µm Carrier: Helium at ml/min 0-0 C at C/min 0 C until compounds elute Injection: Splitless, 0 C Detector: VG Autospec Ultima A :,,, -TCDF :0,,, -TCDF :,,, -TCDF :0 :00 : B :,,, -TCDF :00 : 0: GCES00 Note the separation between,,,-tcdf and,,,-tcdf on DB- is also easily achievable (and actually a little better!) on DB-ms. Congeners in DIn Method pcbs DB-XLB - 0 m x 0. mm, 0.0 µm Carrier: Helium at. cm/sec, measured at 0 C 00 C for min 00-0 C at. C/min Injection: Hot on-column, 0 C Split flow 00 ml/min Detector: MSD, 00 C transfer line SIM of.,.,.,.,.,.,.,. Sample: µl dilute Aroclor mixture BZ BZ BZ () BZ 0 Septum: mm Advanced Green septa, - Liner: Direct connect, single taper, deactivated, mm id, G-00 Syringe: 0 µl tapered, FN -s//hp, - BZ 0 (0) BZ,, BZ BZ 0 BZ 0 BZ BZ BZ BZ GCES00

34 EnVIRonMEnTAL AppLICATIonS, SEMIVoLATILES Extended Temperature program Resolving Congeners and DB-XLB - 0 m x 0. mm, 0.0 µm Carrier: Helium at. cm/sec, measured at 0 C 00 C for min BZ 00- C at. C/min Injection: Hot on-column, 0 C BZ Split flow 00 ml/min BZ BZ, BZ Detector: MSD, 00 C transfer line BZ SIM of.,.,.,.,.,.,.,. 0.0 BZ Sample: µl dilute Aroclor mixture GCES00 BZ BZ pcbs by EpA Method 0 Carrier: DB-ms - 0 m x 0. mm, 0. µm DB-XLB - 0 m x 0. mm, 0.0 µm Helium at cm/sec (EPC in constant flow mode) 0 C for 0. min 0-0 C at C/min 0 C for min Injection: Splitless, 0 C 0 sec purge activation time Detector: µecd, 0 C Nitrogen makeup gas (column + makeup flow = 0 ml/min constant flow) Sample: 0 pg per component DB-ms. IUPAC. Tetrachloro-m-xylene (IS/SS). IUPAC. IUPAC. IUPAC. IUPAC. IUPAC. IUPAC. IUPAC 0 0. IUPAC. IUPAC 0. IUPAC 0. IUPAC. IUPAC. IUPAC. IUPAC. IUPAC. IUPAC 0. IUPAC 0 0. IUPAC 0. Decachlorobiphenyl (IS/SS) IS/SS - Internal Standard/ Surrogate Standard 0 Septum: mm Advanced Green septa, - Liner: Splitless, single taper, deactivated, mm id, - Syringe: 0 µl tapered, FN -s//hp, - DB-XLB GCES00 0

35 EnVIRonMEnTAL AppLICATIonS, SEMIVoLATILES pyrethrins DB m x 0. mm, 0. µm Carrier: Helium at cm/sec, measured at 0 C 0 C for min 0-00 C at 0 C/min 00 C for min 00-0 C at 0 C/min 0 C for min 0- C at 0 C/min C for min Injection: Split, 0 C Split ratio :0 Chromatogram courtesy of Khan Nguyen and Richard Moorman of Sandoz Agro Inc. Detector: FID, 00 C Helium makeup gas at 0 ml/min Sample: µl Heptadecane. Octadecane. Endo-MGK. Exo-MGK. Methoprene. Cinerin I. Jasmolin I. Pyrethrin I. BPE (PB) 0. Cinerin II. Jasmolin II. Pyrethrin II MGK = N-Octylbicycloheptene dicarboximide BPE = Butylcarbityl -propylpiperonyl ether 0 GCES00 organotin Compounds I Carrier: Hp- 0Z-0 m x 0. mm, 0. µm Helium, 00 kpa 0 C for min 0-0 C at C/min Injection: Splitless Detector: AED, 0 C Sample: µl Septum: mm Advanced Green septa, - Liner: Direct connect, single taper, deactivated, mm id, G-00 Syringe: 0 µl tapered, FN -s//hp, - Sn (0 nm) 0 GCES0. Dimethylpentyltin. Tetrabutyltin. Methyltripentyltin. Tributylpentyltin. Dibutylpentyltin. Butlypentyltin. Tetrapentyltin organotin Compounds II Hp- 0J-00 m x 0.0 mm, 0. µm Carrier: Helium, 0. ml/min constant flow 0-0 C at C/min Injection: Splitless, 00 C Detector: AED, 00 C Hg selective at mm Sample: µl Septum: mm Advanced Green septa, - Liner: Direct connect, single taper, deactivated, mm id, G-00 Syringe: 0 µl tapered, FN -s//hp, - Standard Solution Solvent Vent Harbor Sediment Solvent Vent Me SnPh Bu Sn pg Bu SnPh Bu SnPh Bu SnPh pg BuSnPh pg BuSnPh Bu SnPh pg Ph Sn pg Oct SnPh Oct SnPh GCES0

36 EnVIRonMEnTAL AppLICATIonS, SEMIVoLATILES Semivolatile Compounds, US EpA Method 0 Carrier: Hp-ms 0S- 0 m x 0. mm, 0.0 µm Ramped flow. ml/min for 0.0 min Ramp at ml/min to.0 ml/min.0 ml/min for 0. min Ramp at 0 ml/min to. ml/min 0 C for.0 min 0-00 C at C/min 00-0 C at 0 C/min 0-0 C at 0 C/min Septum: mm Advanced Green septa, - Liner: Splitless, single taper, deactivated, mm id, - Syringe: 0 µl tapered, FN -s//hp, - Injection: Splitless, 0 C 0 ml/min purge flow at 0. min Detector: MSD, 0 C transfer line Scan range -00 amu,. scans/sec Sample: µl of 0 ng standard. n-nitrosodimethylamine. Pyridine. -Fluorophenol. Phenol-d. Phenol. Aniline. Bis(-chloroethyl) ether. -Chlorophenol.,-Dichlorobenzene 0.,-Dichlorobenzene-d.,-Dichlorobenzene. Benzyl alcohol.,-dichlorobenzene. -Methylphenol. Bis(-chloroisopropyl) ether. -Methylphenol. n-nitroso-di-n-propylamine. Hexachloroethane. Nitrobenzene-d 0. Nitrobenzene. Isophorone. -Nitrophenol.,-Dimethylphenol. Benzoic acid. Bis(-chloroethoxy) methane.,-dichlorophenol.,,-trichlorobenzene. Naphthalene-d. Naphthalene 0. -Chloroaniline. Hexachlorobutadiene. -Chloro--methylphenol. -Methylnaphthalene. Hexachlorocyclopentadiene.,,-Trichlorophenol,.,,-Trichlorophenol. -Fluorobiphenyl. -Chloronaphthalene. -Nitroaniline 0. Dimethylphthalate.,-Dinitrotoluene. Acenaphthylene. -Nitroaniline. Acenaphthene-d0. Acenaphthene.,-Dinitrophenol. -Nitrophenol. Dibenzofuran.,-Dinitrotoluene 0. Diethylphthalate. -Chlorophenyl-phenyl ether,, Fluorene. -Nitroaniline.,-Dinitro--methylphenol. n-nitrosodiphenylamine. Azobenzene.,,-Tribromophenol. -Bromophenyl-phenylether. Hexachlorobenzene 0. Pentachlorophenol. Phenanthrene-d0. Phenanthrene. Anthracene. Carbazole. Di-n-butylphthalate. Fluoranthene. Pyrene 0, 0. Terphenyl-d. Benzidine 0. Butylbenzylphthalate.,'-Dichlorobenzidine. Benzo[a]anthracene. Chrysene-d. Chrysene. Bis(-ethylhexyl) phthalate. Di-n-octylphthalate. Benzo[b]fluoranthene. Benzo[k]fluoranthene. Benzo[a]pyrene 0. Perylene-d. Indeno[,,-cd]pyrene. Dibenz[a,h]anthracene. Benzo[g,h,i]perylene GCES0 A variety of Agilent HP-ms and DB-ms columns can be used for 0 and similar semivolatiles applications. The column shown above was chosen to maximize inertness and robustness to residues with a thicker 0. µm film, but the price paid is a slightly longer run time. An HP-ms, 0 m x 0. mm id, 0. µm, P/N 0S- would give shorter run times, with slightly less inertness and robustness. A DB-ms, 0 m x 0. mm id, 0. µm, P/N -, would give slightly less inertness, but offer better resolution of PAHs such as Benzo[b]fluoranthene and Benzo[k]fluoranthene. A DB-ms, 0 m x 0. mm x 0. µm, P/N -, can offer significantly reduced run times with a modest loss of inertness.