Thermo Fisher Scientific, San Jose, CA; 2 Kelleher Lab, Northwestern University, Evanston, IL; 3
|
|
- Dominick Fletcher
- 6 years ago
- Views:
Transcription
1 Second-Generation Electron Transfer Dissociation (ETD) on the Thermo Scientific Orbitrap Fusion Mass Spectrometer with Improved Functionality, Increased Speed, and Improved Robustness of Data Christopher Mullen, 1 Lee Earley, 1 Jean-Jacques Dunyach, 1 John E.P. Syka, 1 Philip D. Compton, 2 Dina L. Bai, 3 Jefferey Shabanowitz, 3 and Donald F. Hunt 3 1 Thermo Fisher Scientific, San Jose, CA; 2 Kelleher Lab, Northwestern University, Evanston, IL; 3 Department of Chemistry, University of Virginia, Charlottesville, VA
2 Overview Purpose: Improve ETD robustness, functionality, and speed on the Thermo Scientific Orbitrap Fusion Tribrid mass spectrometer Methods: Orbitrap Fusion mass spectrometer with the Thermo Scientific EASY-ETD ion source Results: Demonstrated increased ETD functionality and usability by using a combination of hardware and software improvements Introduction The Orbitrap Fusion platform incorporates a second-generation EASY-ETD reagent ion source. The EASY-ETD source improvements include a bright and stable glow-dischargebased ETD reagent ion source located between the S-Lens and the Active Beam Guide, and a higher frequency RF axial trapping field (trap end-lens voltage) to improve ion confinement during ETD (Figure 1). The Orbitrap Fusion mass spectrometer design enables previously unavailable parallel/pipelined scan modes to minimize overall scan cycle times. Further, calibration of the ETD reaction kinetics ensures the shortest possible reaction times while maximizing product ion yields and spectral reproducibility. Collectively, these developments constitute a new-generation ETD platform on the Orbitrap Fusion mass spectrometer. Methods Reagent anions from a glow discharge source (previously described) 1,2 are introduced into the ion optics path ahead of the quadrupole mass filter where they are m/z selected, accumulated in the ion-routing multipole, and then transferred into the high pressure trap (HPT) of the dual-pressure linear ion trap for the ETD reaction. Increasing the frequency of the RF axial confinement field during ETD from ½ to 2 times the quadrupole field frequency avoids parametric resonance excitation and ejection of low m/z (typically 1 1 Th) ions. ETD products may be directly transferred to the low pressure trap (LPT) or to the Orbitrap Fusion mass spectrometer for m/z analysis. ITMS 2 ETD scan rates of up to 12 Hz are attainable using a parallel acquisition mode. 2 Second-Generation Electron Transfer Dissociation (ETD) on the Thermo Scientifi c Orbitrap Fusion Mass Spectrometer with Improved Functionality, Increased Speed, and Improved Robustness of Data
3 FIGURE 1. Schematic of the Orbitrap Fusion tribrid mass spectrometer showing the location of the EASY-ETD reagent ion source within the overall ion optics path. The exploded view shows how the reagent ion source is incorporated into the S-Lens/Q region. Ion-routing multipole High-pressure cell produces highly efficient storage of ions Voltages can be adjusted to fragment ions at higher energies Dual-pressure linear ion trap Fragments ions using either CID or optional ETD and provides fast, sensitive mass analysis Ultra-high-field Orbitrap mass analyzer Compact Orbitrap mass spectrometer operating at ultra-high voltage Produces K resolution every 1.2 seconds Ultra-fast operation produces 15 spectra/sec with 15K resolution Active beam guide (ABG) Prevents neutral species from entering Q1 Axial fields improves operational robustness Quadrupole mass filter High Selectivity and excellent transmission C-Trap Ions are focused and injected into the Orbitrap mass spectrometer EASY-ETD ion source Discharge-based source Robust design with an extremely stable source of ions S-Lens High sensitivity Robust ion optics Results Calibrating the reaction kinetics ensures that the ETD fragmentation efficiency is optimized and that the maximum duty cycle for ETD can be accomplished. Calibrating the reaction kinetics is a multi-step process in which the decay of the angiotensin I (433 m/z) precursor is monitored as a function of reaction time at a number of reagent targets (Figure 2). From the slope of the individual decay curves, the reaction rate coefficient is extracted, and plotted as a function of the reagent target at which it was acquired (Figure 3). The data are then fitted to find the target at which the reaction rate coefficient saturates, and combinations of this target with the reaction rate coefficient are used to calculate the optimal charge state dependent reaction times (Table 1). The reaction rate coefficient as a function of the precursor charge state squared has been demonstrated to be linear by J. L. Stephenson Jr. and S. A. McLuckey 3, which is verified in Figure 4, and used to calculate the optimal reaction time per charge state, based on a desired amount of reaction completeness. We find 95% consumption of the initial precursor intensity to yield high quality ETD spectra. Figure 5 shows the relationship between the reaction time and the amount of precursor remaining after reaction for the angiotensin I (433 m/z) precursor. Thermo Scientifi c Poster Note PN HUPO13_POS-3-68_CMullen_E 9/13S 3
4 Results Calibrating the reaction kinetics ensures that the ETD fragmentation efficiency is optimized and that the maximum duty cycle for ETD can be accomplished. Calibrating the reaction kinetics is a multi-step process in which the decay of the angiotensin I (433 m/z) precursor is monitored as a function of reaction time at a number of reagent targets (Figure 2). From the slope of the individual decay curves, the reaction rate coefficient is extracted, and plotted as a function of the reagent target at which it was acquired (Figure 3). The data are then fitted to find the target at which the reaction rate coefficient saturates, and combinations of this target with the reaction rate coefficient are used to calculate the optimal charge state dependent reaction times (Table 1). The reaction rate coefficient as a function of the precursor charge state squared has been demonstrated to be linear by J. L. Stephenson Jr. and S. A. McLuckey 3, which is verified in Figure 4, and used to calculate the optimal reaction time per charge state, based on a desired amount of reaction completeness. We find 95% consumption of the initial precursor intensity to yield high quality ETD spectra. Figure 5 shows the relationship between the reaction time and the amount of precursor remaining after reaction for the angiotensin I (433 m/z) precursor. Precursor Remaining (%) React FIGURE 2. Angiotensen I (433 m/z) precursor decay curves as a function of the reagent anion target under pseudo 1 st order reaction conditions. The reaction progress is monitored for up to four half-lives, and the slope of the individual decays is equal to the negative of the rate coefficient.. FIGURE 6. ETD spectra o of ubiquitin at m/z A) 3.25 msec, the calibra -.5 Ubiquitin_3pt25msec_2e5_uscans #1 RT:. AV: 1 NL: 7.96E6 T: FTMS + p ESI Full ms2 714.@etd3.25 [1.-.] ln(a/a ) Target:.1e5 Target:.2e5 Target:.4e5 Target:.6e5 Target:.7e5 Target:.8e5 Target: 1.e5 Target: 2.e5 Target: 4.e5 Target: 9.9e5-3. Relative Abundance Relative Abundance ubiquitin_8msec_2e5_uscans #1 RT:. AV: 1 NL: 3.24E6 T: FTMS + p ESI Full ms2 714.@etd8. [1.-.] Reaction Time (msec) 4 Second-Generation Electron Transfer Dissociation (ETD) on the Thermo Scientifi c Orbitrap Fusion Mass Spectrometer with Improved Functionality, Increased Speed, and Improved Robustness of Data
5 FIGURE 9. Ion-trap ETD MSM reaction and precursor injec acquisition. The reagent inje msec, corresponding to a rea for all experiments. FIGURE 3. The reaction rate coefficient versus reagent anion target, showing the fit to the data and the optimal reagent anion target leading to at least % of the k max observed k (msec -1 ).4.3 k Fit to Data Best Target x 5 2x 5 3x 5 4x 5 5x 5 Reagent Target TABLE 1. Calculated charge state dependent reaction times based on a saturated reaction rate coefficient of 58.2 sec 1 for charge state 3+, based on 5% of the precursor remaining after reaction. Charge State Reaction Time (msec) FIGURE 8. Ubiquitin sequenc averaging FT micro scans 6.7 seconds. Fragments den (z ion), and green (y ion) aste manual interpretation of the Unique ETD Capabilities The location of the ion-routing Fusion mass spectrometer allo acquisition mode, enabling ITM acquisition rates up to Hz. I rates are slightly reduced due t requirement of the ion-ion reac attainable. In addition, the abili and m/z analysis in parallel wit that the spectral acquisition rat can be maintained for a signific time than in the absence of par Thermo Scientifi c Poster Note PN HUPO13_POS-3-68_CMullen_E 9/13S ime (msec) 5
6 FIGURE 4. The maximum rate coefficient vs. the charge state squared of the precursor is linear 1, allowing extrapolation of the optimal reaction times obtained for a single charge state to all charge states k max (msec -1 ) Ubiquitin Angiotensen Charge State Square (z 2 ) FIGURE 5. Plot of the reaction time required to reduce the reactant precursor by a fixed amount, calculated as a function of the reaction rate coefficient. We find that 3 to 6% remaining precursor is optimal for most compounds. acquisition rates up to H rates are slightly reduced d requirement of the ion-ion attainable. In addition, the and m/z analysis in paralle that the spectral acquisitio can be maintained for a sig time than in the absence o FIGURE 9. Ion-trap ETD M reaction and precursor in acquisition. The reagent msec, corresponding to for all experiments. ETD Reaction Time (msec) Precursor Remaining (%) Rate Coefficient sec -1 sec -1 sec -1 1 Reaction Time (msec) Precursor Inje Conclusion A second-generation ETD source has bee Fusion MS that incor software advanceme Calibration of the rea guesswork from ETD optimizes the ETD re The calibrated reacti provide optimal cond sequence coverage. Parallel acquisition p cycle times of up to 1 References 1.Earley et al., 61 st ASM Spectrometry and All June 9 13, 13; Po Multipurpose Glow D Introduction of Reage Mass Spectrometer, Halls B&C. 2. Earley et al., Present 6 Second-Generation Electron Transfer Dissociation (ETD) on the Thermo Scientifi c Orbitrap Fusion Mass Spectrometer with Improved Functionality, on Increased Mass Spectromet Speed, and Improved Robustness of Data City, Utah, May 23 2
7 Verification of the Calibration The calibrated ETD reaction conditions are verified by using an infusion of ubiquitin from bovine erythrocytes. Spectra of the 12+ charge state at m/z were obtained as a function of the ETD reaction time at an FT resolution of 1K and averaged for micro scans. The spectra were then searched using ProSightPC. Figure 6 shows two representative ubiquitin spectra, obtained at 3.25 msec and 8 msec of reaction time, respectively. The calibrated reaction conditions predict an optimal reaction time of 3.25 msec for the 12+ precursor, and while the spectrum visually looks under reacted, it yields the most total c and z fragments from the ProSightPC v3. search (Figure 7). In addition, we demonstrate the ability to obtain nearly complete ubiquitin sequence coverage on a LC timescale using a combination of ProSightPC searching and manual interpretation of the spectra. The results presented in Figure 8 were achieved by averaging FT micro scans, corresponding to a total acquisition time of 6.7 seconds. FIGURE 6. ETD spectra obtained on the 12+ charge state of ubiquitin at m/z at two different reaction times. A) 3.25 msec, the calibrated reaction time. B) 8 msec. optimizes the ETD react The calibrated reaction c provide optimal condition sequence coverage. Parallel acquisition provi cycle times of up to 12 H References 1.Earley et al., 61 st ASMS Spectrometry and Allied June 9 13, 13; Poster Multipurpose Glow Disch Introduction of Reagent/ Mass Spectrometer, pos Halls B&C. 2.Earley et al., Presented on Mass Spectrometry a City, Utah, May 23 27, 2 3.Stephenson, J. L., Jr. an Chem. Soc. 1996, 118, Ubiquitin_3pt25msec_2e5_uscans #1 RT:. AV: 1 NL: 7.96E6 T: FTMS + p ESI Full ms2 714.@etd3.25 [1.-.] A) ProSightPC is a trademark of Proteinaceous Inc. All oth and its subsidiaries. This information is not intended to encourage use of the intellectual property rights of others. Relative Abundance m/z ubiquitin_8msec_2e5_uscans #1 RT:. AV: 1 NL: 3.24E6 T: FTMS + p ESI Full ms2 714.@etd8. [1.-.] B) Relative Abundance m/z Thermo Scientifi c Poster Note PN HUPO13_POS-3-68_CMullen_E 9/13S 7
8 FIGURE 7. ProSightPC search results for the 12+ charge state of ubiquitin (714.7 m/z) as a function of the ETD reaction time, demonstrating that the calibrated kinetics chooses appropriate reaction times up to at least charge state 12 Number of Fragments 1 c z total ETD Reaction Time (msec) FIGURE 8. Ubiquitin sequence coverage resulting from averaging FT micro scans for a total acquisition time of 6.7 seconds. Fragments denoted by the red (c ion), blue (z ion), and green (y ion) asterisks were found using manual interpretation of the data. Unique ETD Capabilities The location of the ion-routing multipole within the Orbitrap Fusion mass spectrometer allows for a parallel ITMS 2 acquisition mode, enabling ITMS 2 CID and HCD spectral acquisition rates up to Hz. ITMS 2 ETD spectral acquisition rates are slightly reduced due to the additional time requirement of the ion-ion reaction, but rates up to 12 Hz are attainable. In addition, the ability to perform the ion-ion reaction and m/z analysis in parallel with the precursor injection means that the spectral acquisition rate at a particular reaction time can be maintained for a significantly longer precursor injection time than in the absence of parallelization (Figure 9). FIGURE 9. Ion-trap ETD MSMS cycle rate dependence on reaction and precursor injection times using parallel acquisition. The reagent injection time was fixed at 5 msec, corresponding to a reagent anion population of 2e5 for all experiments. ime (msec) Frequency (Hz) 6. 8 Second-Generation Electron Transfer Dissociation (ETD) on the Thermo Scientifi c Orbitrap Fusion Mass Spectrometer with Improved Functionality, Increased Speed, and Improved Robustness of Data 7. 5.
9 time than in the absence of parallelization (Figure 9). FIGURE 9. Ion-trap ETD MSMS cycle rate dependence on reaction and precursor injection times using parallel acquisition. The reagent injection time was fixed at 5 msec, corresponding to a reagent anion population of 2e5 for all experiments. ETD Reaction Time (msec) Frequency (Hz) Precursor Injection Time (msec) Conclusion A second-generation glow-discharge-based EASY- ETD source has been developed for the Orbitrap Fusion MS that incorporates significant hardware and software advancements. Calibration of the reaction kinetics removes the guesswork from ETD, and leads to conditions that optimizes the ETD reaction and scan cycle time. The calibrated reaction conditions are demonstrated to provide optimal conditions for ETD identifications and sequence coverage. Parallel acquisition provides ITMS 2 ETD scan rate cycle times of up to 12 Hz. References 1.Earley et al., 61 st ASMS Conference on Mass Spectrometry and Allied Topics, Minneapolis, MN, June 9 13, 13; Poster Th1 Implementation of a Multipurpose Glow Discharge Ion Source for the Introduction of Reagent/Calibrant Ions Into a Hybrid Mass Spectrometer, poster number: 1, Thursday, Halls B&C. 2.Earley et al., Presented at the 58 th ASMS Conference on Mass Spectrometry and Allied Topics, Salt Lake City, Utah, May 23 27, ; Poster T. 3.Stephenson, J. L., Jr. and McLuckey, S.A. J. Am. Chem. Soc. 1996, 118, A) ProSightPC is a trademark of Proteinaceous Inc. All other trademarks are the property of Thermo Fisher Scientific and its subsidiaries. This information is not intended to encourage use of these products in any manners that might infringe the intellectual property rights of others. Thermo Scientifi c Poster Note PN HUPO13_POS-3-68_CMullen_E 9/13S 9
10 sec -1 sec -1 sec charge state action times.. B) 8 msec. A) Conclusion A second-generation glow-discharge-based EASY- ETD source has been developed for the Orbitrap Fusion MS that incorporates significant hardware and software advancements. Calibration of the reaction kinetics removes the guesswork from ETD, and leads to conditions that optimizes the ETD reaction and scan cycle time. The calibrated reaction conditions are demonstrated to provide optimal conditions for ETD identifications and sequence coverage. Parallel acquisition provides ITMS 2 ETD scan rate cycle times of up to 12 Hz. References Precursor Injection Time (msec) 1.Earley et al., 61 st ASMS Conference on Mass Spectrometry and Allied Topics, Minneapolis, MN, June 9 13, 13; Poster Th1 Implementation of a Multipurpose Glow Discharge Ion Source for the Introduction of Reagent/Calibrant Ions Into a Hybrid Mass Spectrometer, poster number: 1, Thursday, Halls B&C. 2.Earley et al., Presented at the 58 th ASMS Conference on Mass Spectrometry and Allied Topics, Salt Lake City, Utah, May 23 27, ; Poster T. 3.Stephenson, J. L., Jr. and McLuckey, S.A. J. Am. Chem. Soc. 1996, 118, ProSightPC is a trademark of Proteinaceous Inc. All other trademarks are the property of Thermo Fisher Scientific and its subsidiaries. This information is not intended to encourage use of these products in any manners that might infringe the intellectual property rights of others B) Thermo Fisher Scientifi c Inc. All rights reserved. ISO is a trademark of the International Standards Organization. ProSightPC is a trademark of Proteinaceous Inc. All other trademarks are the property of Thermo Fisher Scientifi c, Inc. and its subsidiaries. Specifi cations, terms and pricing are subject to change. Not all products are available in all countries. Please consult your local sales representative for details. Thermo Fisher Scientifi c, San Jose, CA USA is ISO 1:8 Certifi ed. Africa-Other Australia Austria Belgium Canada China Denmark Europe-Other Finland France Germany India Italy Japan Latin America Middle East Netherlands New Zealand Norway Russia/CIS South Africa Spain Sweden Switzerland UK USA HUPO13_POS-3-68_CMullen_E 9/13S
Thermo Fisher Scientific, San Jose, CA; 2 Kelleher Lab, Northwestern University, Evanston, IL; 3
Second-Generation Electron Transfer Dissociation (ETD) on the Thermo Scientific Orbitrap Fusion Mass Spectrometer with Improved Functionality, Increased Speed, Christopher Mullen, 1 Lee Earley, 1 Jean-Jacques
More informationFitting of ETD Rate Constants for Doubly and Triply Charged Ions in a Linear Ion Trap
Fitting of ETD Rate Constants for Doubly and Triply Charged Ions in a Linear Ion Trap Dirk Nolting and Andreas Wieghaus Thermo Fisher Scientific, Bremen, Germany Overview Purpose: Characterize the rate
More informationMultiple Fragmentation Methods for Small Molecule Characterization on a Dual Pressure Linear Ion Trap Orbitrap Hybrid Mass Spectrometer
Application ote: 54 Multiple Fragmentation Methods for Small Molecule Characterization on a Dual Pressure Linear Ion Trap rbitrap Hybrid Mass Spectrometer Kate Comstock, Yingying Huang; Thermo Fisher Scientific,
More informationHR/AM Targeted Peptide Quantification on a Q Exactive MS: A Unique Combination of High Selectivity, High Sensitivity, and High Throughput
HR/AM Targeted Peptide Quantification on a Q Exactive MS: A Unique Combination of High Selectivity, High Sensitivity, and High Throughput Yi Zhang 1, Zhiqi Hao 1, Markus Kellmann 2 and Andreas FR. Huhmer
More informationA Study of Stability, Robustness and Time Efficiency of a New HPLC and a New Tandem MS
A Study of Stability, Robustness and Time Efficiency of a New HPLC and a New Tandem MS Jason Lai, Jia Wang, Brad Hart, Pavel Aronov, Kristine Van Natta, Marta Kozak, Jorge Valdivia, Andy Jacobs, Haibo
More informationIncreasing Speed of UHPLC-MS Analysis Using Single-stage Orbitrap Mass Spectrometer
Increasing Speed of UHPLC-MS Analysis Using Single-stage Orbitrap Mass Spectrometer Olaf Scheibner and Maciej Bromirski Thermo Fisher Scientific, Bremen, Germany Overview Purpose: Improve the performance
More informationImproved Throughput and Reproducibility for Targeted Protein Quantification Using a New High-Performance Triple Quadrupole Mass Spectrometer
Improved Throughput and Reproducibility for Targeted Protein Quantification Using a New High-Performance Triple Quadrupole Mass Spectrometer Reiko Kiyonami, Mary Blackburn, Andreas FR Hühme: Thermo Fisher
More informationA Strategy for an Unknown Screening Approach on Environmental Samples using HRAM Mass Spectrometry
A Strategy for an Unknown Screening Approach on Environmental Samples using HRAM Mass Spectrometry O. Scheibner, 1 P. van Baar, 2 F. Wode, 2 U. Dünnbier, 2 K. Akervik, 3 J. Humphries, 3 M. Bromirski 1
More informationEvaluation of a New HPLC, a New Tandem MS and a New Data Processing Software for General Clinical Use
Evaluation of a New HPLC, a New Tandem MS and a New Data Processing Software for General Clinical Use Shih-Tse Jason Lai, Jia Wang, Brad Hart, Kristine Van Natta, Marta Kozak, Jorge Valdivia, Haibo Wang,
More informationKey Words Q Exactive, Accela, MetQuest, Mass Frontier, Drug Discovery
Metabolite Stability Screening and Hotspot Metabolite Identification by Combining High-Resolution, Accurate-Mass Nonselective and Selective Fragmentation Tim Stratton, Caroline Ding, Yingying Huang, Dan
More informationRapid Quan/Qual Metabolic Stability Analysis with Online Oxidative Metabolism Synthesis
Rapid Quan/Qual Metabolic Stability Analysis with Online Oxidative Metabolism Synthesis Tim Stratton 1, Yingying Huang 1, Katianna Pihakari 1, Ian Acworth 2, and Michael Weber 2 1 Thermo Fisher Scientific,
More informationPlasma-free Metanephrines Quantitation with Automated Online Sample Preparation and a Liquid Chromatography-Tandem Mass Spectrometry Method
Plasma-free Metanephrines Quantitation with Automated Online Sample Preparation and a Liquid Chromatography-Tandem Mass Spectrometry Method Xiang He and Marta Kozak ThermoFisher Scientific, San Jose, CA,
More informationof mass spectrometry
Thermo Scientific 253 Ultra High resolution isotope ratio MS Discover a new world of mass spectrometry Paleoclimatology Atmospheric science Biogeochemistry Petrology Discover the isotopic anatomy of molecules
More informationComplementary Use of Raman and FT-IR Imaging for the Analysis of Multi-Layer Polymer Composites
Complementary Use of Raman and FT-IR Imaging for the Analysis of Multi-Layer Polymer Composites Robert Heintz, Mark Wall, Jennifer Ramirez, Stephan Woods Thermo Fisher Scientific, Madison WI Overview Purpose:
More informationIntroduction to Fourier Transform Infrared Spectroscopy
Introduction to Fourier Transform Infrared Spectroscopy Introduction What is FTIR? FTIR stands for Fourier transform infrared, the preferred method of infrared spectroscopy. In infrared spectroscopy, IR
More informationExploring the Benefits of Automated Unattended Sample Derivatization Prior to Gas Chromatography Analysis
Exploring the Benefits of Automated Unattended Sample Derivatization Prior to Gas Chromatography Analysis A. Caruso, M. Santoro, P. Magni, S. Pelagatti, and R. Facchetti Thermo Fisher Scientific, Milan,
More informationMetWorks Metabolite Identification Software
m a s s s p e c t r o m e t r y MetWorks Metabolite Identification Software Enabling Confident Analysis of Metabolism Data Part of Thermo Fisher Scientific MetWorks Software for the Confident Analysis
More informationSimultaneous, Fast Analysis of Melamine and Analogues in Pharmaceutical Components Using Q Exactive - Benchtop Orbitrap LC-MS/MS
Simultaneous, Fast Analysis of Melamine and Analogues in Pharmaceutical Components Using Q Exactive - Benchtop Orbitrap LC-MS/MS Kate Comstock, Tim Stratton, Hongxia (Jessica) Wang, and Yingying Huang
More informationHigh-Pressure Electrolytic Carbonate Eluent Generation Devices and Their Applications in Ion Chromatography Systems
High-Pressure Electrolytic Carbonate Eluent Generation Devices and Their Applications in Ion Chromatography Systems Yan Liu, Zhongqing Lu, and Chris Pohl; Thermo Fisher Scientific, Sunnyvale, CA USA Overview
More informationA Platform to Identify Endogenous Metabolites Using a Novel High Performance Orbitrap MS and the mzcloud Library
A Platform to Identify Endogenous Metabolites Using a Novel High Performance Orbitrap MS and the mzcloud Library Junhua Wang, 1 David A. Peake, 1 Robert Mistrik, 2 Yingying Huang 1 1 Thermo Fisher Scientific
More informationImproved Screening for 250 Pesticides in Matrix using a LC-Triple Quadrupole Mass Spectrometer
Improved Screening for 2 Pesticides in Matrix using a LC-Triple Quadrupole Mass Spectrometer Mary lackburn, Jia Wang, Jonathan eck, Charles Yang, Dipankar Ghosh, Thermo Fisher Scientific, San Jose, C,
More informationA Strategy for an Unknown Screening Approach on Environmental Samples Using HRAM Mass Spectrometry
A Strategy for an Unknown Screening Approach on Environmental Samples Using HRAM Mass Spectrometry Olaf Scheibner, 1 Patrizia van Baar, 2 Florian Wode, 2 Uwe Dünnbier, 2 Kristi Akervik, 3 Jamie Humphrie,
More informationImproving Intact Antibody Characterization by Orbitrap Mass Spectrometry
Improving Intact Antibody Characterization by Orbitrap Mass Spectrometry Kai Scheffler, 1 Eugen Damoc, 2 Mathias Müller, 2 Martin Zeller, 2 Thomas Moehring 2 Thermo Fisher Scientific, Dreieich 1 and Bremen,
More informationThermo Scientific ELEMENT GD PLUS Glow Discharge Mass Spectrometer. Defining quality standards for the analysis of solid samples
Thermo Scientific ELEMENT GD PLUS Glow Discharge Mass Spectrometer Defining quality standards for the analysis of solid samples Redefine your quality standards for the elemental analysis of solid samples
More informationCharacterization of Polymers and Plastics (pellets, powders and films) by the Thermo Scientific FLASH 2000 Elemental Analyzer
Characterization of Polymers and Plastics (pellets, powders and films) by the Thermo Scientific FLASH 000 Elemental Analyzer Dr. Liliana Krotz and Dr. Guido Giazzi Thermo Fisher Scientific, Milan, Italy
More informationUtility of H-SRM to Reduce Matrix Interference in Food Residue Analysis of Pesticides by LC-MS/MS Using the TSQ Quantum Discovery
Application Note: 3 Utility of H-SRM to Reduce Matrix Interference in Food Residue Analysis of Pesticides by LC-MS/MS Using the TSQ Quantum Discovery Yoko Yamagishi, Thermo Fisher Scientific, C-2F 3-9
More informationInsights Into the Nanoworld Analysis of Nanoparticles with ICP-MS
Insights Into the Nanoworld Analysis of Nanoparticles with ICP-MS Daniel Kutscher, 1 Jörg Bettmer, 2 Torsten Lindemann, 1 Shona McSheehy-Ducos, 1 Lothar Rottmann 1 1 Thermo Fisher Scientific, Germany 2
More informationNew Multi-Collector Mass Spectrometry Data for Noble Gases Analysis
New Multi-Collector Mass Spectrometry Data for Noble Gases Analysis Alessandro Santato, 1 Doug Hamilton, 1 Jan Wijbrans, 2 Claudia Bouman 1 1 Thermo Fisher Scientific, Bremen, Germany 2 VU University Amsterdam,
More informationExploring Mixed-Mode Chromatography Column Chemistry, Properties, and Applications
Exploring Mixed-Mode Chromatography Column Chemistry, Properties, and Applications Xiaodong Liu and Christopher Pohl; Thermo Fisher Scientific, Sunnyvale, CA Overview Review mixed-mode column technology
More informationIntroduction to Fourier Transform Infrared Spectroscopy
molecular spectroscopy Introduction to Fourier Transform Infrared Spectroscopy Part of Thermo Fisher Scientific Introduction What is FT-IR? FT-IR stands for Fourier Transform InfraRed, the preferred method
More informationHigh-Field Orbitrap Creating new possibilities
Thermo Scientific Orbitrap Elite Hybrid Mass Spectrometer High-Field Orbitrap Creating new possibilities Ultrahigh resolution Faster scanning Higher sensitivity Complementary fragmentation The highest
More informationHILIC Method Development in a Few Simple Steps
HILIC Method Development in a Few Simple Steps Monica Dolci, Luisa Pereira, Dafydd Milton and Tony Edge Thermo Fisher Scientific, Runcorn, Cheshire, UK Overview This poster presents a systematic approach
More informationActive Flow Technology Understanding How the Flow Rate Profile Affects the Chromatographic Efficiency
Active Flow Technology Understanding How the Flow Rate Profile Affects the Chromatographic Efficiency Anthony Edge, 1 Luisa Pereira, 1 Dafydd Milton 1 and Andrew Shalliker 2 1 Thermo Fisher Scientific,
More informationHigh-Resolution Accurate-Mass (HRAM) Phthalate Screening using Direct Analysis in Real Time (DART) Ambient Ionization
igh-resolution Accurate-Mass (RAM) Phthalate Screening using Direct Analysis in Real Time (DART) Ambient Ionization Catharina Crone, 1 Markus Kellmann, 1 Yue Xuan, 1 Elizabeth Crawford 2 1 Thermo Fisher
More informationMonitoring Protein PEGylation with Ion Exchange Chromatography
Monitoring Protein PEGylation with Ion Exchange Chromatography Peter Yu, Deanna Hurum, Jinyuan (Leo) Wang, 2 Terry Zhang, 2 and Jeffrey Rohrer Thermo Fisher Scientific, Sunnyvale, CA; 2 Thermo Fisher Scientific,
More informationDirect Analysis using Paper-Spray Mass Spectrometry: Method Development for the Rapid Screening of Drugs of Abuse for Forensic Toxicology
Direct Analysis using Paper-Spray Mass Spectrometry: Method Development for the Rapid Screening of Drugs of Abuse for Forensic Toxicology Maria C. Prieto Conaway, 1 Nicholas E. Manicke, 2 Marta Kozak 1
More informationThe Raman Spectroscopy of Graphene and the Determination of Layer Thickness
Application Note: 52252 The Raman Spectroscopy of Graphene and the Determination of Layer Thickness Mark Wall, Ph.D., Thermo Fisher Scientific, Madison, WI, USA Key Words DXR Raman Microscope 2D Band D
More informationAccelerated Solvent Extraction GC-MS Analysis and Detection of Polycyclic Aromatic Hydrocarbons in Soil
Accelerated Solvent Extraction GC-MS Analysis and Detection of Polycyclic Aromatic Hydrocarbons in Soil Che Jinshui, 1 Deng Guifeng, 1 Liang Lina, 1 and Aaron Kettle, 2 1 Thermo Fisher Scientific (China)
More informationfor XPS surface analysis
Thermo Scientific Avantage XPS Software Powerful instrument operation and data processing for XPS surface analysis Avantage Software Atomic Concentration (%) 100 The premier software for surface analysis
More informationFast, Effective XPS Point Analysis of Metal Components
Application Note: 52297 Fast, Effective XPS Point Analysis of Metal Components Chris Baily and Tim Nunney, Thermo Fisher Scientific, East Grinstead, West Sussex, UK Key Words K-Alpha Auto-Analysis Multi-Spectrum
More informationThermo Scientific LTQ Orbitrap Velos Hybrid FT Mass Spectrometer
IET International Equipment Trading Ltd. www.ietltd.com Proudly serving laboratories worldwide since 1979 CALL +847.913.0777 for Refurbished & Certified Lab Equipment Thermo Scientific LTQ Orbitrap Velos
More informationThermo Scientific ConFlo IV Universal Interface. Continuous Flow Interface. Isotope Ratio MS
Thermo Scientific ConFlo IV Universal Interface Continuous Flow Interface Isotope Ratio MS 3 ConFlo IV Universal Interface for Continuous Flow Isotope Ratio MS The development of Continuous Flow carrier
More informationQuan/Qual Analyses. Unmatched Confidence for. Thermo Scientific Q Exactive Orbitrap LC-MS/MS System. Identify Quantify Confirm
26.1645 165.91 33.267 Thermo Scientific Q Exactive rbitrap LC-MS/MS System Unmatched Confidence for Quan/Qual Analyses Identify Quantify Confirm Quanfirmation \ kwän-f r-'m -shän\ n 1 : the ability to
More informationAnalyzing Residual Solvents in Pharmaceutical Products Using GC Headspace with Valve-and-Loop Sampling
Analyzing Residual Solvents in Pharmaceutical Products Using GC Headspace with Valve-and-Loop Sampling Andrea Caruso and Massimo Santoro, Thermo Fisher Scientific, Milan, Italy Application Note 1316 Key
More informationThermo Scientific Pesticide Explorer Collection. Start-to-finish. workflows for pesticide analysis
Thermo Scientific Pesticide Explorer Collection Start-to-finish workflows for pesticide analysis Comprehensive Pesticide Analysis Solutions Pesticide Explorer Collection Selection table Lab Profile Routine
More informationKey Words Q Exactive Focus, Orbitrap, veterinary drugs, small molecule HRAM quantitation, small molecule HRAM screening, UHPLC, vdia
Variable Data-Independent Acquisition (vdia) Delivers High Selectivity and Sensitivity in Combined Targeted and Untargeted Analyses for Small Molecules Olaf Scheibner 1, Markus Kellmann 1, Charles Yang
More informationTargeted protein quantification
Targeted Quantitative Proteomics Targeted protein quantification with high-resolution, accurate-mass MS Highly selective Very sensitive Complex samples HR/AM A more complete quantitative proteomics picture
More informationAn Evaluation of Various High-Resolution, Accurate-Mass Scan Modes for In Vitro Drug Discovery Screening
An Evaluation of Various High-Resolution, Accurate-Mass Scan Modes for In Vitro Drug Discovery Screening Jonathan McNally, 1 Nicholas Duczak Jr., 1 Patrick Bennett, 1 Francois Espourteille, and Maciej
More informationThermo Scientific K-Alpha + XPS Spectrometer. Fast, powerful and accessible chemical analysis for surface and thin film characterization
Thermo Scientific K-Alpha + XPS Spectrometer Fast, powerful and accessible chemical analysis for surface and thin film characterization X-ray Photoelectron Spectroscopy Quantitative, chemical identification
More informationDetermination of BTEX in Cigarette Filter Fibers Using GC-MS with Automated Calibration
Determination of BTEX in Cigarette Filter Fibers Using GC-MS with Automated Calibration Zhang Xuebin 1, Yu Chongtian 1, Liang Lina 1, Hans-Joachim Huebschmann 2, Thermo Fisher Scientific Shanghai, 1 China,
More informationQuantitative and Qualitative Confirmation of Pesticides in Beet Extract Using a Hybrid Quadrupole-Orbitrap Mass Spectrometer
Quantitative and Qualitative Confirmation of Pesticides in Beet Extract Using a Hybrid Quadrupole-Orbitrap Mass Spectrometer Charles Yang and Dipankar Ghosh, Thermo Fisher Scientific, San Jose, CA Olaf
More informationDeterminations of Inorganic Anions and Organic Acids in Beverages Using Suppressed Conductivity and Charge Detection
Determinations of Inorganic Anions and Organic Acids in Beverages Using Suppressed Conductivity and Charge Detection Terri Christison, Linda Lopez Thermo Fisher Scientific, Sunnyvale, CA, USA Overview
More informationDetermination of Tetrafluoroborate, Perchlorate, and Hexafluorophosphate in a Simulated Electrolyte Sample from Lithium Ion Battery Production
Determination of Tetrafluoroborate, Perchlorate, and Hexafluorophosphate in a Simulated Electrolyte Sample from Lithium Ion Battery Production Thunyarat Phesatcha, Suparerk Tukkeeree, Jeff Rohrer 2 Thermo
More informationFast and Reliable Method for the Analysis of Methylmalonic Acid from Human Plasma
Fast and Reliable Method for the Analysis of Methylmalonic Acid from Human Plasma Jon Bardsley 1, James Goldberg 2 1 Thermo Fisher Scientific, Runcorn, UK; 2 Thermo Fisher Scientific, West Palm Beach,
More informationIncreasing the Multiplexing of Protein Quantitation from 6- to 10-Plex with Reporter Ion Isotopologues
Increasing the Multiplexing of Protein Quantitation from 6- to 1-Plex with Reporter Ion Isotopologues Rosa Viner, 1 Ryan Bomgarden, 2 Michael Blank, 1 John Rogers 2 1 Thermo Fisher Scientific, San Jose,
More informationFrank Steiner, Michael Heidorn, and Markus M. Martin Thermo Fisher Scientific, Germering, Germany
Generic Method Approach for Pharmaceutical Drug Discovery and Development using Reversed-Phase Hydrophilic Interaction Liquid Chromatography with Universal Charged Aerosol Detection Frank Steiner, Michael
More informationDetection of Mycotoxins in Corn Meal Extract Using Automated Online Sample Preparation with Liquid Chromatography-Tandem Mass Spectrometry
Detection of Mycotoxins in Corn Meal Extract Using Automated Online Sample Preparation with Liquid Chromatography-Tandem Mass Spectrometry Yang Shi, Catherine Lafontaine, Timothy B. Haney, and François
More informationSemi-Targeted Screening of Pharmaceutically- Related Contaminants in the Thames Tideway using LC-HRMS
Semi-Targeted Screening of Pharmaceutically- Related Contaminants in the Thames Tideway using LC-HRMS Kelly Munro, 1 Anthony Edge, 2 Claudia Martins, 3 David Cowan 4 and Leon Barron 1 1 Analytical & Environmental
More informationLC-MS/MS Method for the Determination of Diclofenac in Human Plasma
LC-MS/MS Method for the Determination of Diclofenac in Human Plasma J. Jones, Thermo Fisher Scientific, Runcorn, Cheshire, UK Application Note 20569 Key Words SPE, SOLA, Accucore RP-MS, diclofenac, Core
More informationInterazioni di ioni con elettroni (ECD, ETD) e fotoni (Ion spectroscopy) Gianluca Giorgi. via Aldo Moro Siena
Interazioni di ioni con elettroni (ECD, ETD) e fotoni (Ion spectroscopy) Gianluca Giorgi Università degli Studi di Siena Dipartimento di Biotecnologie, Chimica e Farmacia via Aldo Moro 53100 Siena e-mail:
More informationIN QUALITATIVE ANALYSIS,
IN QUALITATIVE ANALYSIS, YOU VE ALWAYS HAD TO CHOOSE BETWEEN ACCURACY AND THROUGHPUT. NOW THERE S NO REASON TO ever have to. WATERS QUALITATIVE ANALYSIS solutions Waters ACQUITY UPLC System with the Waters
More informationTypes of Analyzers: Quadrupole: mass filter -part1
16 Types of Analyzers: Sector or double focusing: magnetic and electric Time-of-flight (TOF) Quadrupole (mass filter) Linear ion trap Quadrupole Ion Trap (3D trap) FTICR fourier transform ion cyclotron
More informationCharles Yang, Dipankar Ghosh, Jonathan Beck Thermo Fisher Scientific, San Jose, CA, USA
Utilization of High Resolution LC-MS for Screening and Quantitative Analysis of Pesticides in Food Matrix using a Q Exactive Benchtop Orbitrap Platform Charles Yang, Dipankar Ghosh, Jonathan Beck Thermo
More informationTomorrow s quantitation with the TSQ Fortis mass spectrometer: quantitation of phenylephrine hydrochloride for QA/QC laboratories
APPLICATION NOTE 65200 Tomorrow s quantitation with the TSQ Fortis mass spectrometer: quantitation of phenylephrine hydrochloride for QA/QC laboratories Authors Neloni Wijeratne, Claudia Martins, Mary
More informationHydrophilic Interaction Liquid Chromatography: Method Development Approaches
Hydrophilic Interaction Liquid Chromatography: Method Development Approaches Monica Dolci, Luisa Pereira, and Tony Edge Thermo Fisher Scientific, Runcorn, Cheshire, UK Abstract This poster summarizes the
More informationKey Words Nanoparticles, spicp-ms, ICP-MS, Qtegra ISDS Software, AF4-ICP-MS
Nanoparticle Characterization Via Single Particle Inductively Coupled Plasma Mass Spectrometry (spicp-ms) Using a Dedicated Plug-in for Qtegra ISDS Software Technical Note 43279 Daniel Kutscher, Julian
More informationScreening Method for 30 Pesticides in Green Tea Extract Using Automated Online Sample Preparation with LC-MS/MS
Application Note: 514 Screening Method for 3 Pesticides in Green Tea Extract Using Automated Online Sample Preparation with LC-MS/MS Yang Shi, Catherine Lafontaine, Thermo Fisher Scientific, Franklin,
More informationXPS Surface Characterization of Disposable Laboratory Gloves and the Transfer of Glove Components to Other Surfaces
Application Note: 52287 XPS Surface Characterization of Disposable Laboratory Gloves and the Transfer of Glove Components to Other Surfaces Brian R. Strohmeier, Thermo Fisher Scientific, Madison, WI, USA
More informationAccurate measurement of elemental impurities in metals and metal alloys using the Thermo Scientific icap TQ ICP-MS
APPLICATION NOTE 434 Accurate measurement of elemental impurities in metals and metal alloys using the Thermo Scientific icap TQ ICP-MS Authors Introduction Marcus Manecki, Daniel Kutscher, Shona McSheehy
More informationRelative quantification using TMT11plex on a modified Q Exactive HF mass spectrometer
POSTER NOTE 6558 Relative quantification using TMT11plex on a modified mass spectrometer Authors Tabiwang N. Arrey, 1 Rosa Viner, 2 Ryan D. Bomgarden, 3 Eugen Damoc, 1 Markus Kellmann, 1 Thomas Moehring,
More informationQuantitation and Characterization of Copper Plating Bath Additives by Liquid Chromatography with Charged Aerosol Detection
Quantitation and Characterization of Copper Plating Bath Additives by Liquid Chromatography with Charged Aerosol Detection Marc Plante, Bruce Bailey, Ian N. Acworth Thermo Fisher Scientific, Chelmsford,
More informationMass Selective Ejection by Axial Resonant Excitation from a Linear Ion Trap
Mass Selective Ejection by Axial Resonant Excitation from a Linear Ion Trap Yuichiro Hashimoto, Hideki Hasegawa, Takashi Baba, and Izumi Waki Central Research Laboratory, Hitachi, Ld., Tokyo, Japan We
More informationHigh-Throughput LC-MS/MS Quantification of Estrone (E1) and Estradiol (E2) in Human Blood Plasma/Serum for Clinical Research Purposes
High-Throughput LC-MS/MS Quantification of Estrone (E1) and Estradiol (E2) in Human Blood Plasma/Serum for Clinical Research Purposes Joe DiBussolo, Marta Kozak Thermo Fisher Scientific, San Jose, CA Application
More informationComparison of Solid Core HPLC Column Performance: Effect of Particle Diameter
Comparison of Solid Core HPLC Column Performance: Effect of Particle Diameter Luisa Pereira, Thermo Fisher Scientific, Runcorn, Cheshire, UK Technical Note 20755 Key Words Solid core, fused core, superficially
More informationAchieve confident synthesis control with the Thermo Scientific ISQ EC single quadrupole mass spectrometer
APPLICATION NOTE 72385 Achieve confident synthesis control with the Thermo Scientific ISQ EC single quadrupole mass spectrometer Authors Stephan Meding, Katherine Lovejoy, Martin Ruehl Thermo Fisher Scientific,
More informationMethod Development for a Simple and Reliable Determination of PCBs in Mineral Insulating Oil by SPME-GC-ECD
Method Development for a Simple and Reliable Determination of PCBs in Mineral Insulating Oil by SPME-GC-ECD Massimo Santoro, 1 Sergio Guazzotti, 1 Danilo Pierone, 2 Alexandre Souza, 3 Jaqueline Lorena,
More informationMASS ANALYSER. Mass analysers - separate the ions according to their mass-to-charge ratio. sample. Vacuum pumps
ION ANALYZERS MASS ANALYSER sample Vacuum pumps Mass analysers - separate the ions according to their mass-to-charge ratio MASS ANALYSER Separate the ions according to their mass-to-charge ratio in space
More informationARGUS VI. Static Vacuum Mass Spectrometer. Static Vacuum ARGUS VI. Multicollection Low Volume Precision
ARGUS VI Static Vacuum Mass Spectrometer Static Vacuum ARGUS VI Multicollection Low Volume Precision Based on more than 20 years of experience in noble gas mass spectrometry instrumentation, we have developed
More informationAutomated and accurate component detection using reference mass spectra
TECHNICAL NOTE 72703 Automated and accurate component detection using reference mass spectra Authors Barbara van Cann 1 and Amit Gujar 2 1 Thermo Fisher Scientific, Breda, NL 2 Thermo Fisher Scientific,
More informationUtility of the Charge Detector in Ion Chromatography Applications
Utility of the Charge Detector in Ion Chromatography Applications Mrinal K. Sengupta, Sheetal Bhardwaj, Kannan Srinivasan, Chris Pohl, and Purnendu K. Dasgupta Thermo Fisher Scientific, Sunnyvale, California,
More informationThermo Scientific LTQ Velos Dual-Pressure Linear Ion Trap
m a s s s p e c t r o m e t r y Thermo Scientific LTQ Velos Dual-Pressure Linear Ion Trap World's fastest and most sensitive ion trap mass spectrometer Part of Thermo Fisher Scientific Thermo Scientific
More informationComplete Materials Deformulation Using TGA-IR
Application Note: 51694 Complete Materials Deformulation Using TGA-IR Michael Bradley, Thermo Fisher Scientific, Madison, WI, USA Key Words Feedstock Supplies Finished Products Multi-component Search OMNIC
More informationTranslational Biomarker Core
Translational Biomarker Core Instrumentation Thermo Scientific TSQ Quantum Triple Quadrupole Mass Spectrometers. There are two TSQ Quantum Ultra AM instruments available in the TBC. The TSQ Quantum Ultra
More informationDefining quality standards for the analysis of solid samples
Defining quality standards for the analysis of solid samples Thermo Scientific Element GD Plus Glow Discharge Mass Spectrometer Redefine your quality standards for the elemental analysis of solid samples
More informationHydrophilic Interaction Liquid Chromatography: Some Aspects of Solvent and Column Selectivity
Hydrophilic Interaction Liquid Chromatography: Some Aspects of Solvent and Column Selectivity Monica Dolci, Thermo Fisher Scientific, Runcorn, Cheshire, UK Technical Note 20544 Key Words Hydrophilic, HILIC,
More informationprotein interaction analysis bulletin 6300
protein interaction analysis bulletin 6300 Guide to SPR Data Analysis on the ProteOn XPR36 System Ruben Luo, Bio-Rad Laboratories, Inc., 2000 Alfred Nobel Drive, Hercules, CA 94547 Kinetic Analysis To
More information( )( ) Selectivity Choices in Reversed-Phase Fast LC. Introduction. R s = 1 a 1 k 4 a 1 + k
Selectivity Choices in Reversed-Phase Fast LC Luisa Pereira, Monica Dolci, Thermo Fisher Scientific, Runcorn, Cheshire, UK Technical Note 20543 Key Words Accucore, Hypersil GOLD, Syncronis, Column Chemistry,
More informationThermo Finnigan LTQ. Specifications
IET International Equipment Trading Ltd. www.ietltd.com Proudly serving laboratories worldwide since 1979 CALL +847.913.0777 for Refurbished & Certified Lab Equipment Thermo Finnigan LTQ Specifications
More informationFORENSIC TOXICOLOGY SCREENING APPLICATION SOLUTION
FORENSIC TOXICOLOGY SCREENING APPLICATION SOLUTION A purpose-built collection of the best-inclass components for forensic toxicology Whether you re challenged to present reliable and secure forensic sample
More informationHELIX MC Plus. Static Vacuum. ARGUS VI Thermo Scientific HELIX MC Plus Static Vacuum Mass Spectrometer Static Vacuum Mass Spectrometer
ARGUS VI Thermo Scientific HELIX MC Plus Static Vacuum Mass Spectrometer Static Vacuum Mass Spectrometer Static Vacuum HELIX MC Plus Dynamic range Multi collection High mass resolution Based on more than
More informationImpurity Profiling of Pharmaceutical Starting Materials Using Gas Chromatography Coupled with High- Resolution Accurate Mass Spectrometry
Impurity Profiling of Pharmaceutical Starting Materials Using Gas Chromatography Coupled with High- Resolution Accurate Mass Spectrometry Cristian Cojocariu and Paul Silcock Thermo Fisher Scientific, Runcorn,
More informationCharged Aerosol Detection and Evaporative Light Scattering Detection Fundamental Differences Affecting Analytical Performance
Charged Aerosol Detection and Evaporative Light Scattering Detection Fundamental Differences Affecting Analytical Performance David Thomas, Bruce Bailey, Marc Plante and Ian Acworth Thermo Fisher Scientific,
More informationThis document is a preview generated by EVS
TECHNICAL REPORT RAPPORT TECHNIQUE TECHNISCHER BERICHT CEN/TR 15641 August 2007 ICS 67.050 English Version Food analysis - Determination of pesticide residues by LC- MS/MS - Tandem mass spectrometric parameters
More informationAdvanced Fragmentation Techniques for BioPharma Characterization
Advanced Fragmentation Techniques for BioPharma Characterization Global BioPharma Summit The world leader in serving science Different modes of fragmentation to answer different questions or for different
More informationA novel high resolution accurate mass Orbitrap-based GC-MS platform for routine analysis of Short Chained Chlorinated Paraffins
TECHNICAL NOTE 39 A novel high resolution accurate mass Orbitrap-based GC-MS platform for routine analysis of Short Chained Chlorinated Paraffins Author Cristian Cojocariu Thermo Fisher Scientific, Runcorn,
More informationThe key to your laboratory s success
The key to your laboratory s success Food Safety and Environmental Application Training Invest in Yourself People are the most valuable assets in any lab. We offer comprehensive, professional training
More informationδ 13 C of DIC and carbonate samples: comparison of traditional mass spectrometry methods with an infrared spectrometry method
APPLICATION NOTE 30486 δ 13 C of DIC and carbonate samples: comparison of traditional mass spectrometry methods with an infrared spectrometry method Authors Danijela Smajgl, 1 Magda Mandic, 1 Dirk Nürnberg,
More informationCarbonyl-Reactive Tandem Mass Tag (TMT) Reagents for Mass Spectrometry-Based Quantitative Glycomics
Carbonyl-Reactive Tandem Mass Tag (TMT) Reagents for Mass Spectrometry-Based Quantitative Glycomics Sergei I. Snovida, 1 Rosa Viner, 2 John C. Rogers 1 1 Thermo Fisher Scientific, Rockford, IL; 2 Thermo
More informationIntroduction to the Q Trap LC/MS/MS System
www.ietltd.com Proudly serving laboratories worldwide since 1979 CALL +1.847.913.0777 for Refurbished & Certified Lab Equipment ABI Q Trap LC/MS/MS Introduction to the Q Trap LC/MS/MS System The Q Trap
More information(Refer Slide Time 00:09) (Refer Slide Time 00:13)
(Refer Slide Time 00:09) Mass Spectrometry Based Proteomics Professor Sanjeeva Srivastava Department of Biosciences and Bioengineering Indian Institute of Technology, Bombay Mod 02 Lecture Number 09 (Refer
More information