Candice E. Paulsen, Ph.D.

Transcription

1 Candice E. Paulsen, Ph.D. Yale University 266 Whitney Ave New Haven, CT Phone: (203) EDUCATION University of Michigan Sept Ph.D. Chemical Biology Thesis: Elucidating the role of protein sulfenylation in eukaryotic signal transduction Page 1 of 5 Purdue University May 2006 B.S. Genetic Biology RESEARCH EXPERIENCE Assistant Professor, Yale University January 2018 Present Staff Scientist, Yale University November 2017 December 2017 Advisor: establishing my own lab Post-doctoral Fellow, July 2012 October 2017 Department of Physiology Advisor: David Julius Initiated a collaboration with Dr. Yifan Cheng to explore structural biology of TRPA1 Determined de novo structure of the TRPA1 ion channel by electron cryo-microscopy (Cryo-EM) Identified the site and mechanism for polyanion regulation of TRPA1 Uncovered the three-dimensional position of key cysteine residues involved in electrophile detection, which suggests activation mechanism Developed biochemical methods to purify homogenous, well-behaved TRPA1 This work resulted in a first author publication in Nature. This work was funded through NIH T-32 and Helen Hay Whitney postdoctoral fellowships. Post-doctoral Associate, Sept June 2012 Department of Chemistry Advisor: Kate Carroll Developed a radioactive assay to study redox modulation of the Epidermal Growth Factor Receptor (EGFR) kinase domain Developed a membrane preparation assay to study kinase activity of EGFR This work resulted in 2 publications in Chemical Reviews and Cell Chemical Biology. Graduate Student, University of Michigan, Ann Arbor July 2006 Sept Chemical Biology Graduate Program Advisor: Kate Carroll Demonstrated that the yeast glutathione peroxidase, Gpx3 communicates oxidative stress to the Yap1 transcription factor through a sulfenic acid intermediate Uncovered graded redox modulation of the EGFR kinase and 3 phosphatases by hydrogen peroxide produced as a second messenger during EGF signaling Developed methodology to study global and targeted cysteine sulfenylation in response to signaling cascades in live eukaryotic cells This work resulted in 3 first author publications in Nature Chemical Biology, Chemistry & Biology, and ACS Chemical Biology and was funded through a Chemistry-Biology Interface NIH training grant. FELLOWSHIPS Howard Hughes Medical Institute Fellow of the Helen Hay Whitney Foundation

2 Role: Postdoctoral Fellow $154,000 NIH Cardiovascular Research Institute T Role: Postdoctoral Fellow $39,000 Chemistry-Biology Interface NIH T-32 Training Grant Role: Graduate Student $33,600 HONORS & AWARDS Travel Award, PECRUM Symposium, $ Travel Award, VAUGHN Symposium, $ PEER-REVIEWED PUBLICATIONS 1. van der Wijst, J. Leunissen, E.H., Blanchard, M.G., Venselaar, H., Verkaart, S., Paulsen, C.E., Bindels, R.J., Hoenderop, J.G. A gate hinge controls the epithelial calcium channel TRPV5, Science Reports, 7: 45489, Truong, T.H., Ung, P.M., Palde, P.B., Paulsen, C.E., Sclessinger, A., Carroll, K.S. Molecular basis for redox activation of epidermal growth factor receptor kinase, Cell Chemical Biology, 23: , Paulsen, C. E.*, Armache, J.P.*, Gao, Y., Cheng, Y., Julius, D. Structure of the human TRPA1 ion channel suggests regulatory mechanisms, Nature, 520: , (*contributed equally to this study) Highlighted in: Clapham, D.E. Structural biology: Pain-sensing TRPA1 channel resolved, Nature, 520: , citations 4. Paulsen, C.E., Carroll, K.S. Cysteine-Based Redox Signaling: Chemistry, Biology, and Tools for Discovery, Chemical Reviews 113: , citations 5. Paulsen, C.E., Truong, T.H., Garcia, F.J., Homann, A., Gupta, V., Leonard, S.E., Carroll, K.S. Peroxidedependent sulfenylation of the EGFR catalytic site enhances kinase activity, Nature Chemical Biology, 8: 57-64, citations 6. Paulsen, C.E., Carroll, K.S. Orchestrating redox signaling networks through regulatory cysteine switches, ACS Chemical Biology, 5: 47-62, citations 7. Paulsen, C.E., Carroll, K.S. Chemical dissection of an essential redox switch in yeast, Chemistry & Biology, 16: , citations TEACHING EXPERIENCE Guest lecturer, University of Michigan Spring 2010 Course: Chemical Biology Introductory Course Page 2 of 5

3 Responsibilities: Invited to teach one lecture on post-translational modifications, including my graduate work to graduate students. Developed and delivered a 60-minute lecture to a class of 20. Graduate Teaching Assistant, University of Michigan Spring 2009 Course: Chemical Biology Introductory Course Responsibilities: Graded exams and quizzes. Held office hours. Lead a paper discussion session for 20 graduate students. Graduate Teaching Assistant, University of Michigan Fall 2007, 2008 Course: Organic Chemistry 1 & 2 Responsibilities: Supervised two 3-hour laboratory sessions with undergraduate students each. Held weekly office hours. Developed quiz and lab report assignments. Proctored and graded exams. General and Organic Chemistry Tutor, Purdue University Spring 2005 Spring 2006 Location: Purdue Chemistry Resource Room Responsibilities: Assist first and second-year undergraduate students with their homework and helped review lecture materials and exams. MENTORING EXPERIENCE Mentor for John V. King, UCSF graduate student Spring 2014 Fall 2017 Current status: Graduate student in the Julius lab Mentor for Yuan Gao, UCSF graduate student Spring 2013 Summer 2017 Current status: Postdoctoral fellow in Rapoport lab, Harvard Medical School Mentor for Thu H. Truong, University of Michigan graduate student Spring 2005-Summer 2012 Current status: Postdoctoral fellow in the Lange lab, University of Minnesota SERVICE & OUTREACH Rackham Graduate School Student Council University of Michigan CELLebration Booth Attendant Bay Area Science Festival Booth Attendant Expert Lecturer on the Science of Peppers and Mint July 2015 Exploratorium After Dark Event, San Francisco, CA Presenter on the Science of Peppers and Mint Jan California Academy of Science Nightlife Event, San Francisco, CA Co-Ad Hoc Reviewer Since 2014 Cell, Nature Ad Hoc Reviewer 2013 Bioorganic & Medicinal Chemistry and Bioorganic & Medicinal Chemistry Letters PRESENTATIONS Oral Presentations Page 3 of 5

4 1. Structure of the human TRPA1 ion channel suggests regulatory mechanisms Ion Channels Gordon Research Conference, South Hadley, MA, Structure of the human TRPA1 ion channel suggests regulatory mechanisms Biophysical Society Annual Meeting, Los Angeles, CA, Structure of the human TRPA1 ion channel suggests regulatory mechanisms Helen Hay Whitney Fellowship Retreat, Dedham, MA, Activation and Regulatory Mechanisms: Insights From Structures of TRPV1 and TRPA1 Invited speaker, TRP Channel Symposium, Leuven, Belgium, Sulfenylation Goes Global: Probing Intracellular Targets of Hydrogen Peroxide Produced for Growth Factor Signaling Chemistry Department Seminar,, Painting the Cysteine Chapel: New Tools to Probe Oxidation Biology ASBMB Annual Meeting, Anaheim, CA, A Chemical Biology Approach to Probing the Yap1-Gpx3 Redox Relay System in Yeast PECRUM Symposium, University of Michigan, Ann Arbor, MI, A Chemical Biology Approach to Probing the Yap1-Gpx3 Redox Relay System in Yeast Life Sciences Institute Symposium, University of Michigan, Ann Arbor, MI, Poster Presentations 1. Paulsen, C.E., Carroll, K.S. Identification of the Protein Targets of Hydrogen Peroxide Produced for EGF Signaling 22 nd Enzyme Mechanisms Conference, St. Petersburg, FL, Paulsen, C.E., Carroll, K.S. Chemical Dissection of an Essential Redox Switch in Yeast and Identification of the Protein Targets of Hydrogen Peroxide Produced for EGF Signaling Thiol-Based Redox Regulation and Signaling Gordon Conference, Lucca, Italy, Paulsen, C.E., Carroll, K.S. Chemical Dissection of an Essential Redox Switch in Yeast and Identification of the Protein Targets of Hydrogen Peroxide Produced for EGF Signaling Chemical-Biology Interface Symposium, Ann Arbor, MI, Paulsen, C.E., Carroll, K.S. Chemical Dissection of an Essential Redox Switch in Yeast. American Chemical Society National Meeting, Washington, DC, Paulsen, C.E., Carroll, K.S. Chemical Dissection of an Essential Redox Switch in Yeast. VAUGHN Symposium, University of Michigan, Ann Arbor, MI, 2009 AFFILIATIONS & MEMBERSHIPS Alpha Chi Sigma, professional chemistry fraternity Since 2003 American Chemical Society 2007 American Heart Association 2013 Biophysical Society present Page 4 of 5

5 REFERENCES David Julius, Ph.D. Professor and Chair, Department of Physiology Yifan Cheng, Ph.D. HHMI Investigator Professor, Department of Biochemistry & Biophysics Kate Carroll, Ph.D. Associate Professor, Department of Chemistry Katrin Karbstein, Ph.D. Associate Professor, Dept. of Cancer Biology Page 5 of 5