For Online. Review Only. Kenneth Carter UMASS 2005
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1 For nline iopatterned Review nly Surfaces Carter Research Group University of Massachusetts
2 iopatterned Surfaces For nline Review nly Protein chips anomolding embed functionality into feature - use LFRP to to incorporate functionality
3 iopatterned Surfaces = = Streptavidin For nline iotinylated iomolecule ( e.g., peptides, DA, or proteins ) Review nly Wash away excess avidin and iotin patterned surface Incubation in aq. soln. of avidin incubate with a biotin conjugate of the biomolecule Qi, K.; Wooley, K. L.; Jhaveri, S..; Sogah, D. Y.; einhoff, M.; Malkoch, M.; Carter, K. R.; awker, C. J. PMSE Prep., 2004, awker, C. J.; Wooley, K. L.; Carter, K. R.; Sogah, D. Y.; Qi, K.; Jhaveri, S..; einhoff, M.; Malkoch, M. PMSE Prep., 2004, 90, 279. Surface patterned with biomolecules
4 Functionality via itroxide Exchange itroxide Exchange: - ased on equilibrium between dormant alkoxyamine and active nitroxide Alkoxyamine within within crosslinked polymer Added Added free free functional alkoxyamine in in solution For nline. +. Review nly Exchange between o C nitroxides 120 o C + ( very small %) ( Large excess ) Poly Functional group group exchanged onto onto the the growing brush brush ends ends
5 Functionality via itroxide Exchange Embedded Alkoxyamine Initiators = Desired Functional Group to be introduced on the surface For nline Review nly Polymerize brushes in in the presence of of functional alkoxyamine Exchanged Functional group group Polymer brushes control control solvation
6 Example: iotinylated Surfaces Example 1: iotin - A iological Ligand Cr 4 For nline a 3 Review nly r DMF LiAl 4 PPh 3 S iotin-s ester DMF, Et 3 iotin functionality is covalently attached to the nitroxide part of the alkoxyamine TF 3 2 Qi, K.; Wooley, K. L.; Jhaveri, S..; Sogah, D. Y.; einhoff, M.; Malkoch, M.; Carter, K. R.; awker, C. J. PMSE Prep., 2004, awker, C. J.; Wooley, K. L.; Carter, K. R.; Sogah, D. Y.; Qi, K.; Jhaveri, S..; einhoff, M.; Malkoch, M. PMSE Prep., 2004, 90, 279.
7 ioresponsive Surfaces Run# 1 Silicon / Quartz substrate For nline Me 2. Review nly 125 o C DM F Sub. Silicon S 1.16 M n 8.75 K PDI Alexa Flor labelled streptavidin 10 mm EPES buffer PDMA no iotin iotinylated PDMA Qi, K.; Wooley, K. L.; Jhaveri, S..; Sogah, D. Y.; einhoff, M.; Malkoch, M.; Carter, K. R.; awker, C. J. PMSE Prep., 2004, awker, C. J.; Wooley, K. L.; Carter, K. R.; Sogah, D. Y.; Qi, K.; Jhaveri, S..; einhoff, M.; Malkoch, M. PMSE Prep., 2004, 90, 279. Scale bar = 100um
8 Fluorescence Images 100 um For nline Review nly Scale bar = 100um Selective attachment of of labeled streptavadin! Individual nm nm lines lines could could not not be be resolved due due to to limitations of of confocal microsxopy 40 um Qi, K.; Wooley, K. L.; Jhaveri, S..; Sogah, D. Y.; einhoff, M.; Malkoch, M.; Carter, K. R.; awker, C. J., Manuscript in Preparation, 2005
9 IgE / Dinitrophenol Interactions 40 um For nline (1) First IgE 10 um Review Treatment nly 10 um Qi, K.; Wooley, K. L.; Jhaveri, S..; Sogah, D. Y.; einhoff, M.; Malkoch, M.; Carter, K. R.; awker, C. J., Manuscript in Preparation, um 10 um (2) DP-SA apten Detachment Treatments (3) Second IgE Treatment
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