For Online. Review Only. Kenneth Carter UMASS 2005

Size: px
Start display at page:

Download "For Online. Review Only. Kenneth Carter UMASS 2005"

Transcription

1 For nline iopatterned Review nly Surfaces Carter Research Group University of Massachusetts

2 iopatterned Surfaces For nline Review nly Protein chips anomolding embed functionality into feature - use LFRP to to incorporate functionality

3 iopatterned Surfaces = = Streptavidin For nline iotinylated iomolecule ( e.g., peptides, DA, or proteins ) Review nly Wash away excess avidin and iotin patterned surface Incubation in aq. soln. of avidin incubate with a biotin conjugate of the biomolecule Qi, K.; Wooley, K. L.; Jhaveri, S..; Sogah, D. Y.; einhoff, M.; Malkoch, M.; Carter, K. R.; awker, C. J. PMSE Prep., 2004, awker, C. J.; Wooley, K. L.; Carter, K. R.; Sogah, D. Y.; Qi, K.; Jhaveri, S..; einhoff, M.; Malkoch, M. PMSE Prep., 2004, 90, 279. Surface patterned with biomolecules

4 Functionality via itroxide Exchange itroxide Exchange: - ased on equilibrium between dormant alkoxyamine and active nitroxide Alkoxyamine within within crosslinked polymer Added Added free free functional alkoxyamine in in solution For nline. +. Review nly Exchange between o C nitroxides 120 o C + ( very small %) ( Large excess ) Poly Functional group group exchanged onto onto the the growing brush brush ends ends

5 Functionality via itroxide Exchange Embedded Alkoxyamine Initiators = Desired Functional Group to be introduced on the surface For nline Review nly Polymerize brushes in in the presence of of functional alkoxyamine Exchanged Functional group group Polymer brushes control control solvation

6 Example: iotinylated Surfaces Example 1: iotin - A iological Ligand Cr 4 For nline a 3 Review nly r DMF LiAl 4 PPh 3 S iotin-s ester DMF, Et 3 iotin functionality is covalently attached to the nitroxide part of the alkoxyamine TF 3 2 Qi, K.; Wooley, K. L.; Jhaveri, S..; Sogah, D. Y.; einhoff, M.; Malkoch, M.; Carter, K. R.; awker, C. J. PMSE Prep., 2004, awker, C. J.; Wooley, K. L.; Carter, K. R.; Sogah, D. Y.; Qi, K.; Jhaveri, S..; einhoff, M.; Malkoch, M. PMSE Prep., 2004, 90, 279.

7 ioresponsive Surfaces Run# 1 Silicon / Quartz substrate For nline Me 2. Review nly 125 o C DM F Sub. Silicon S 1.16 M n 8.75 K PDI Alexa Flor labelled streptavidin 10 mm EPES buffer PDMA no iotin iotinylated PDMA Qi, K.; Wooley, K. L.; Jhaveri, S..; Sogah, D. Y.; einhoff, M.; Malkoch, M.; Carter, K. R.; awker, C. J. PMSE Prep., 2004, awker, C. J.; Wooley, K. L.; Carter, K. R.; Sogah, D. Y.; Qi, K.; Jhaveri, S..; einhoff, M.; Malkoch, M. PMSE Prep., 2004, 90, 279. Scale bar = 100um

8 Fluorescence Images 100 um For nline Review nly Scale bar = 100um Selective attachment of of labeled streptavadin! Individual nm nm lines lines could could not not be be resolved due due to to limitations of of confocal microsxopy 40 um Qi, K.; Wooley, K. L.; Jhaveri, S..; Sogah, D. Y.; einhoff, M.; Malkoch, M.; Carter, K. R.; awker, C. J., Manuscript in Preparation, 2005

9 IgE / Dinitrophenol Interactions 40 um For nline (1) First IgE 10 um Review Treatment nly 10 um Qi, K.; Wooley, K. L.; Jhaveri, S..; Sogah, D. Y.; einhoff, M.; Malkoch, M.; Carter, K. R.; awker, C. J., Manuscript in Preparation, um 10 um (2) DP-SA apten Detachment Treatments (3) Second IgE Treatment

Enrichment Media and Kits

Enrichment Media and Kits Enrichment Media and Kits Innovative Biotin/Streptavidin-free Tools for Capturing Azide- or Alkyne-tagged Proteins Enrichment Media and Kits Protein Enrichment Kits Far superior digest purity compared

More information

SPHERO TM Magnetic Particles

SPHERO TM Magnetic Particles SPHER TM Particles SPHER TM Microparticles provide high quality and reproducible results for your application Allow for rapid and reliable binding between the target and magnetic particle Consists of a

More information

SPHERO TM Magnetic Particles

SPHERO TM Magnetic Particles 56 27845 Irma Lee Circle, Lake Forest, IL 645 SPHER TM Magnetic SPHER TM Magnetic Microparticles provide high quality and reproducible results for your application Allow for rapid and reliable binding

More information

Supporting Information for. PNA FRET Pair Miniprobes for Quantitative. Fluorescent in Situ Hybridization to Telomeric DNA in Cells and Tissue

Supporting Information for. PNA FRET Pair Miniprobes for Quantitative. Fluorescent in Situ Hybridization to Telomeric DNA in Cells and Tissue Supporting Information for PNA FRET Pair Miniprobes for Quantitative Fluorescent in Situ Hybridization to Telomeric DNA in Cells and Tissue Orenstein, et al Page S2... PNA synthesis procedure Page S3...

More information

Nanotube Molecular Transporters

Nanotube Molecular Transporters Nanotube Molecular Transporters N a n o c h e m i s t ry P r e s e n t a t i o n H s i a o - N u n g C h e n C h e m i s t ry & b i o c h e m i s t ry d e p a rt m e n t U t a h s t a t e u n i v e r s

More information

Supporting Information

Supporting Information Supporting Information Energy Transfer-Based Multiplexed Assay of Proteases by Using Gold Nanoparticle and Quantum Dot Conjugates on a Surface Young-Pil Kim, 1, Young-Hee Oh, 1, Eunkyu Oh, 1 Sungho Ko,

More information

Scaling up Microwave Reactions

Scaling up Microwave Reactions Scaling up Microwave Reactions Presented by Joe Pawluczyk Preparative Chemistry and Separations Application of Modern Tools in rganic Synthesis Biotage Summer Program July 21 23, 2008 University of Richmond

More information

magnetic micro- and nanoparticles

magnetic micro- and nanoparticles micromod Partikeltechnologie GmbH modular designed Technological Applications Publications and Reviews magnetic micro- and nano Implementation in Life Sciences Product overview 10 nm 100 nm 1 µm 10 µm

More information

Copyright WILEY-VCH Verlag GmbH, D Weinheim, Supporting Information for Angew. Chem. Int. Ed. Z 18050

Copyright WILEY-VCH Verlag GmbH, D Weinheim, Supporting Information for Angew. Chem. Int. Ed. Z 18050 Copyright WILEY-VCH Verlag GmbH, D-69451 Weinheim, 2001. Supporting Information for Angew. Chem. Int. Ed. Z 18050 Protein Affinity Labeling Mediated by Genetically Encoded Peptide Tags Frank Amini, Thomas

More information

Mouse Anti-OVA-IgE ELISA KIT

Mouse Anti-OVA-IgE ELISA KIT Mouse Anti-OVA-IgE ELISA KIT Research Reagent Please, read this instruction carefully before use. This is an ELISA (Enzyme Linked ImmunoSorbent Assay) kit for measurement of mouse anti-ova (ovalbumin)-ige

More information

Gold nanoparticles from Innova Biosciences

Gold nanoparticles from Innova Biosciences Gold nanoparticles from Innova Biosciences A unique range of options Innova Biosciences Guide Innova Biosciences Ltd. Babraham Research Campus, Cambridge, UK, CB22 3AT +44 (0)1223 661000 info@innovabiosciences.com

More information

Supporting Information DIELS-ALDER CLICKABLE POLYMER BRUSHES: A VERSATILE CATALYST-FREE CONJUGATION PLATFORM

Supporting Information DIELS-ALDER CLICKABLE POLYMER BRUSHES: A VERSATILE CATALYST-FREE CONJUGATION PLATFORM Supporting Information DIELS-ALDER CLICKABLE PLYMER BRUSHES: A VERSATILE CATALYST-FREE CJUGATI PLATFRM Yasemin ursel Yuksekdag, Tugce ihal Gevrek, Amitav Sanyal* Department of Chemistry, Bogazici University,

More information

SPHERO TM Magnetic Particles

SPHERO TM Magnetic Particles SPHER TM Magnetic Particles SPHER TM Magnetic Microparticles provide high quality and reproducible results for your application Allow for rapid and reliable binding between the target and magnetic particle

More information

Gold Nanoparticles product guide. Innovative Reagents & Services for Life Sciences & Diagnostics

Gold Nanoparticles product guide. Innovative Reagents & Services for Life Sciences & Diagnostics Gold Nanoparticles product guide Innovative Reagents & Services for Life Sciences & Diagnostics INDEX Introduction...3 Colloidal Gold...3 Conjugation Kits...4 Gold nanoparticle conjugates...5 InnovaCoat

More information

Degree of labeling (DOL)

Degree of labeling (DOL) Degree of labeling (DOL) A written explanation for the determination of the degree of labeling when using NuLink reagents. The DOL is the average number of reagent molecules that have been covalently attached

More information

Alkaline Phosphatase Labeling Kit-NH2

Alkaline Phosphatase Labeling Kit-NH2 Alkaline Phosphatase Labeling Kit-NH2 Catalog Number KA0001 1 Kit Version: 02 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 Principle of the Assay...

More information

Stable Encapsulation of Quantum Dot Barcodes with Silica Shells

Stable Encapsulation of Quantum Dot Barcodes with Silica Shells Stable Encapsulation of Quantum Dot Barcodes with Silica Shells Shang-Hsiu Hu and Xiaohu Gao Department of Bioengineering, University of Washington Seattle, WA 98195 (USA) Adv. Funct. Mater. 2010. ASAP

More information

Spherotech, Inc Irma Lee Circle, Unit 101, Lake Forest, Illinois PARTICLE COATING PROCEDURES

Spherotech, Inc Irma Lee Circle, Unit 101, Lake Forest, Illinois PARTICLE COATING PROCEDURES SPHERO TM Technical Note STN-1 Rev C. 041106 Introduction Currently, there are several methods of attaching biological ligands to polystyrene particles. These methods include adsorption to plain polystyrene

More information

Supporting Information. Time-Resolved Botulinum Neurotoxin A Activity Monitored using. Peptide-Functionalized Au Nanoparticle Energy Transfer Sensors

Supporting Information. Time-Resolved Botulinum Neurotoxin A Activity Monitored using. Peptide-Functionalized Au Nanoparticle Energy Transfer Sensors Electronic Supplementary Material (ESI) for Chemical Science. This journal is The Royal Society of Chemistry 2014 Supporting Information Time-Resolved Botulinum Neurotoxin A Activity Monitored using Peptide-Functionalized

More information

Supplementary information

Supplementary information Supplementary information Optimization of PEG coating conditions Coating conditions were optimized in order to address the full effect of different lengths of PEG polymer. A ligand exchange was made in

More information

Moscow Institute of Physics and Technology, Institutsky 9, Dolgoprudny , Russia 2

Moscow Institute of Physics and Technology, Institutsky 9, Dolgoprudny , Russia 2 Graphene oxide linking layers: a versatile platform for biosensing Yu.V. Stebunov 1, O.A. Aftenieva 1, A.V. Arsenin 1, and V.S. Volkov 1,2 1 Moscow Institute of Physics and Technology, Institutsky 9, Dolgoprudny

More information

Applications and Properties of New Polymeric Mixed Mode Cation Exchange Chromatography Media

Applications and Properties of New Polymeric Mixed Mode Cation Exchange Chromatography Media Applications and Properties of New Polymeric Mixed Mode Cation Exchange Chromatography Media Nandu Deorkar*, Wei Guo, Bob Buss, Joe Mladosich and Paul Bouis Mallinckrodt Baker Inc. 1904 J.T. Baker Way

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION Methods: Poly(zwitterionic)protein conjugates offer increased stability without sacrificing binding affinity or bioactivity Andrew J. Keefe and Shaoyi Jiang Materials: α-chymotrypsin from bovine pancreas

More information

Sphere Scientific Corporation MonoPS Microspheres Our standard polystyrene microspheres are extremely uniform with an excellent lot-to-lot reproducibility. Those microspheres are mainly devoted to hydrophobic

More information

Electronic Supplementary Information. Continuous Flow Microfluidic-MS System for Efficient OBOC Screening

Electronic Supplementary Information. Continuous Flow Microfluidic-MS System for Efficient OBOC Screening Electronic Supplementary Material (ESI) for RSC Advances. This journal is The Royal Society of Chemistry 2014 Electronic Supplementary Information Continuous Flow Microfluidic-MS System for Efficient OBOC

More information

ATTO 565 and ATTO 590

ATTO 565 and ATTO 590 ATTO 565 and ATTO 590 General Information ATTO 565 and ATTO 590 are fluorescent labels belonging to the well known rhodamine dyes. A common feature of all rhodamine dyes is a carboxyphenyl substituent

More information

Novel Water-Soluble Near-Infrared Cyanine Dyes: Synthesis, Spectral Properties, and Use in the Preparation of Internally Quenched Fluorescent Probes

Novel Water-Soluble Near-Infrared Cyanine Dyes: Synthesis, Spectral Properties, and Use in the Preparation of Internally Quenched Fluorescent Probes ovel Water-Soluble ear-infrared Cyanine Dyes: Synthesis, Spectral Properties, and Use in the Preparation of Internally Quenched Fluorescent Probes Cédric Bouteiller,,, Guillaume Clavé,,, Aude Bernardin,,

More information

Supplemental Information for: Mechanical properties of a complete microtubule revealed through molecular dynamics simulation

Supplemental Information for: Mechanical properties of a complete microtubule revealed through molecular dynamics simulation Supplemental Information for: Mechanical properties of a complete microtubule revealed through molecular dynamics simulation avid Wells and Aleksei Aksimentiev Water placement and histidine protonation

More information

Nanopores: Solid-state nanopores for these experiments were produced by using the

Nanopores: Solid-state nanopores for these experiments were produced by using the Materials and Methods Nanopores: Solid-state nanopores for these experiments were produced by using the highly focused electron beam of a transmission electron microscope (TEM) to drill a single pore in

More information

SS Vorlesung Polymermaterialien Polymerisationsmethoden

SS Vorlesung Polymermaterialien Polymerisationsmethoden Professur Polymerchemie SS 2017 Vorlesung Prof. Michael Sommer 1 www.tu-chemnitz.de Content Free radical polymerization (PS, PMMA) Controlled radical polymerization Ionic polymerization (cationic, anionic)

More information

One-Step Functionalization of Zwitterionic Poly[(3- (methacryloylamino)propyl)dimethyl(3-sulfopropyl)ammonium

One-Step Functionalization of Zwitterionic Poly[(3- (methacryloylamino)propyl)dimethyl(3-sulfopropyl)ammonium Electronic Supplementary Material (ESI) for Chemical Communications. This journal is The Royal Society of Chemistry 2014 Supporting Information One-Step Functionalization of Zwitterionic Poly[(3- (methacryloylamino)propyl)dimethyl(3-sulfopropyl)ammonium

More information

Supporting Information

Supporting Information Supporting Information Calculation of the swelling ratios of DN hydrogels To estimate χ parameter for PAAm water system, eqs 1a and 1b were solved using the experimentally determined swelling ratios and

More information

Supporting information

Supporting information Electronic Supplementary Material (ESI) for Journal of Materials Chemistry C. This journal is The Royal Society of Chemistry 2015 Supporting information Bioconjugation of hydrogen-bonded organic semiconductors

More information

POLY Awards Mark Scholars -Previous Awardees Mark Scholars Program

POLY Awards Mark Scholars -Previous Awardees Mark Scholars Program 2015 Mark Scholars Program Mark Scholar Award: Stuart Rowan Mark Young Scholar Award: Bradley Olsen 2013 Mark Scholars Program Mark Young Scholar Award: Rachel O'Reilly Mark Scholar Award : Rigoberto Advincula

More information

Mouse Insulin ELISA KIT S-Type

Mouse Insulin ELISA KIT S-Type Mouse Insulin ELISA KIT S-Type Research Reagent Please, read this instruction carefully before use. This is an ELISA (Enzyme Linked ImmunoSorbent Assay) kit for measurement of mouse insulin with high specificity

More information

Incubation time too short Incubate samples overnight at 4 C or follow the manufacturer guidelines.

Incubation time too short Incubate samples overnight at 4 C or follow the manufacturer guidelines. Poor standard curve Improper standard solution Standard improperly reconstituted Standard degraded Curve doesn't fit scale Pipetting error Confirm dilutions are made correctly. Briefly spin vial before

More information

Monkey Kidney injury molecule 1,Kim-1 ELISA Kit

Monkey Kidney injury molecule 1,Kim-1 ELISA Kit Monkey Kidney injury molecule 1,Kim-1 ELISA Kit Catalog No: E0785Mo 96 Tests Operating instruction www.eiaab.com FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH

More information

Surface-modified Nonwoven Membranes with High Capacity for Protein Binding. P V Gurgel, H Liu, Y Zheng, and R G Carbonell

Surface-modified Nonwoven Membranes with High Capacity for Protein Binding. P V Gurgel, H Liu, Y Zheng, and R G Carbonell Surface-modified Nonwoven Membranes with High Capacity for Protein Binding P V Gurgel, H Liu, Y Zheng, and R G Carbonell Nonwoven Fabrics High-speed, low cost processes 300-1,000 meters/minute, 5-6 meters

More information

Supporting Information

Supporting Information Supporting Information To Engineered Holliday Junctions as Single-Molecule Reporters for Protein-DNA Interactions with Application to a MerR-family Regulator Susanta K. Sarkar, a Nesha May Andoy, a Jaime

More information

Canine brain natriuretic peptide,bnp ELISA Kit

Canine brain natriuretic peptide,bnp ELISA Kit Canine brain natriuretic peptide,bnp ELISA Kit Catalog No: E0541c 96 Tests Operating instruction www.eiaab.com FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH

More information

Data sheet. UV-Tracer TM Biotin-Maleimide. For Labeling of Thiol-groups with UV-detectable Biotin CLK-B Description

Data sheet. UV-Tracer TM Biotin-Maleimide. For Labeling of Thiol-groups with UV-detectable Biotin CLK-B Description Cat. No. CLK-B105-10 CLK-B105-100 Amount 10 mg 100 mg For in vitro use only! Quality guaranteed for 12 months Store at -20 C 1. Description UV-Tracer TM Biotin Maleimide for biotinylation reactions of

More information

Application Note Antibody-SOMAmer Sandwich Assay

Application Note Antibody-SOMAmer Sandwich Assay Application Note Antibody-SOMAmer Sandwich Assay Introduction SOMAmer reagents (Slow Off-rate Modified Aptamers) are DNA-based high affinity (average Kd < 1 nm) protein binding reagents with proprietary

More information

Thin film techniques: the layer-by-layer self assembly technique

Thin film techniques: the layer-by-layer self assembly technique Thin film techniques: the layer-by-layer self assembly technique Carmelina Ruggiero University of Genoa Overview Thin films Thin film techniques Langmuir-Blodgett technique Chemical self-assembling Layer-by-Layer

More information

Switching shape of hollow layer-by-layer hydrogel microcontainers

Switching shape of hollow layer-by-layer hydrogel microcontainers Electronic Supplementary Information Switching shape of hollow layer-by-layer hydrogel microcontainers Veronika Kozlovskaya, William Higgins, Jun Chen and Eugenia Kharlampieva* University of Alabama at

More information

Protocol for Coating QD-COOH on glass slides Chris Ochs 19/09/12 Modified by Kathy Lu 2/27/2013

Protocol for Coating QD-COOH on glass slides Chris Ochs 19/09/12 Modified by Kathy Lu 2/27/2013 Protocol for Coating QD-COOH on glass slides cjochs@smart.mit.edu Chris Ochs 19/09/12 Modified by Kathy Lu 2/27/2013 kalu@ucsd.edu Cleaning glass slides prior to coupling and Amination with APTS (Aminopropyl

More information

the spatial arrangement of atoms in a molecule and the chemical bonds that hold the atoms together Chemical structure Covalent bond Ionic bond

the spatial arrangement of atoms in a molecule and the chemical bonds that hold the atoms together Chemical structure Covalent bond Ionic bond Chemical structure the spatial arrangement of atoms in a molecule and the chemical bonds that hold the atoms together Covalent bond bond formed by the sharing of valence electrons between atoms Ionic bond

More information

Technology background

Technology background DS004 Technology background ontent: QTL Technology Introduction 1 Polymer-Polymer Ensembles and Their Application to Biosensing 2 Multiplexed Detection Using Mixtures ontaining Supported Polymer 3 Supported

More information

Human papillomavirus,hpv ELISA Kit

Human papillomavirus,hpv ELISA Kit Human papillomavirus,hpv ELISA Kit Catalog No: E0787h 96 Tests Operating instructions www.eiaab.com FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH ENTIRE PROCEDURE

More information

ERT320 BIOSEPARATION ENGINEERING CHROMATOGRAPHY

ERT320 BIOSEPARATION ENGINEERING CHROMATOGRAPHY ERT320 BIOSEPARATION ENGINEERING CHROMATOGRAPHY CHROMATOGRAPHY Week 9-10 Reading Assignment: Chapter 7. Bioseparations Science & Engineering, Harrison, R; Todd, P; Rudge, S.C and Petrides, D,P CHROMATOGRAPHY

More information

Reversible Interaction between Substrate and Ligand

Reversible Interaction between Substrate and Ligand Reversible Interaction between Substrate and Ligand 2010.6.9.YoheiShimizu(D3) is is is Ser 271 P Lys 229-2 3 P Zn 2+ Tyr P 3 2- + 3 Lys 107 P 3 2- class 1 aldolase class 2 aldolase Glu 185 Asp 211 C 2

More information

Answers Chem 250 Unit 1 Proteomics by Mass Spectrometry

Answers Chem 250 Unit 1 Proteomics by Mass Spectrometry Answers Chem 250 Unit 1 Proteomics by Mass Spectrometry Article #1 Quantitative MS for proteomics: teaching a new dog old tricks. MacCoss MJ, Matthews DE., Anal Chem. 2005 Aug 1;77(15):294A-302A. 1. Questions

More information

Name: Student Number:

Name: Student Number: Page 1 of 5 ame: Student umber: l University of Manitoba - Department of Chemistry 2.222 - Introductory rganic Chemistry II - Term Test 2 Thursday, March 9, 2006 This is a 2-hour test, marked out of 48

More information

From Fragments to Actin: The Bistramide A Story. March 12 th, 2010

From Fragments to Actin: The Bistramide A Story. March 12 th, 2010 From Fragments to Actin: The Bistramide A Story March 12 th, 2010 Act I: The Protagonist Synthesis of Bistramide A Gillis, Wang, Davis, Fujii, Bromann Bistramide A Biological Activity Cell Cycle Regulation

More information

Philipp Gramlich (Autor) Selective DNA Modification Using the Cu(I)-Catalyzed Alkyne- Azide Cycloaddition

Philipp Gramlich (Autor) Selective DNA Modification Using the Cu(I)-Catalyzed Alkyne- Azide Cycloaddition Philipp Gramlich (Autor) Selective DNA Modification Using the Cu(I)-Catalyzed Alkyne- Azide Cycloaddition https://cuvillier.de/de/shop/publications/1334 Copyright: Cuvillier Verlag, Inhaberin Annette Jentzsch-Cuvillier,

More information

In-Solution Digestion: Multi-Plate v1.0 Quick Start Guide

In-Solution Digestion: Multi-Plate v1.0 Quick Start Guide In-Solution Digestion: Multi-Plate v1.0 Quick Start Guide This guide is for users who have been trained in the proper use of the AssayMAP Bravo Platform and understand the safety guidelines in the Bravo

More information

Human prolactin, PRL ELISA Kit

Human prolactin, PRL ELISA Kit Human prolactin, PRL ELISA Kit Catalog No: E0846h 96 Tests Operating instruction www.eiaab.com FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH ENTIRE PROCEDURE

More information

Counteranion-Mediated Intrinsic Healing of Poly(Ionic Liquid) Copolymers

Counteranion-Mediated Intrinsic Healing of Poly(Ionic Liquid) Copolymers Supporting Information Counteranion-Mediated Intrinsic Healing of Poly(Ionic Liquid) Copolymers Panlong Guo, Houyu Zhang, Xiaokong Liu and Junqi Sun* State Key Laboratory of Supramolecular Structure and

More information

Use of shape induced birefringence for rotation in optical tweezers

Use of shape induced birefringence for rotation in optical tweezers Use of shape induced birefringence for rotation in optical tweezers Theodor Asavei, Timo A. Nieminen, Norman R. Heckenberg, Halina Rubinsztein-Dunlop School of Mathematics and Physics, The University of

More information

Supporting Information

Supporting Information Supporting Information Tuning Supramolecular Structure and Functions of Peptide bola-amphiphile by Solvent Evaporation-Dissolution Anhe Wang,, Lingyun Cui,, Sisir Debnath, Qianqian Dong, Xuehai Yan, Xi

More information

Protease-Triggered Unveiling of Bioactive Nanoparticles

Protease-Triggered Unveiling of Bioactive Nanoparticles Protease-Triggered Unveiling of Bioactive Nanoparticles Todd J. Harris, Geoffrey von Maltzahn, Matthew E. Lord, Ji-Ho Park, Amit Agrawal, Dal-Hee Min, Michael J. Sailor, *Sangeeta N. Bhatia SUPPORTING

More information

Human amyloid beta peptide 1-42, Aβ1-42 ELISA Kit

Human amyloid beta peptide 1-42, Aβ1-42 ELISA Kit Human amyloid beta peptide 1-42, Aβ1-42 ELISA Kit Catalog No: E0946h 96 Tests Operating instruction www.eiaab.com FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH

More information

Use of Silica and Iron-Oxide Nanoparticles with Specific Surface Coatings for Enhanced Oil Recovery

Use of Silica and Iron-Oxide Nanoparticles with Specific Surface Coatings for Enhanced Oil Recovery Texas Use of Silica and Iron-Oxide Nanoparticles with Specific Surface Coatings for Enhanced Oil Recovery Chun Huh Dept. of Petroleum & Geosystems Engineering University of Texas at Austin IOR Norway 2015:

More information

Synthesis of Nanoparticles and Surface Modifications

Synthesis of Nanoparticles and Surface Modifications Synthesis of Nanoparticles and Surface Modifications Self-Assembly Static assembly Dynamic assembly RT = 8.314 J/mol x 300 = 2.4 kj/mol Driving forces Chemisorption Surface effect Hydrophobic-hydrophilic

More information

Supplementary Materials

Supplementary Materials DNA-Linker Induced Surface Assembly of Ultra Dense Parallel Single Walled Carbon Nanotube Arrays Si-ping Han,, Hareem T. Maune, #, Robert D. Barish,, Marc Bockrath,@ and William A. Goddard III, 3 * Materials

More information

Name Class Date. KEY CONCEPT All living things are based on atoms and their interactions. atom ion molecule

Name Class Date. KEY CONCEPT All living things are based on atoms and their interactions. atom ion molecule Section 1: Atoms, Ions, and Molecules KEY CONCEPT All living things are based on atoms and their interactions. VOCABULARY atom ion molecule element ionic bond compound covalent bond MAIN IDEA: Living things

More information

In Situ Gelation-Induced Death of Cancer Cells Based on Proteinosomes

In Situ Gelation-Induced Death of Cancer Cells Based on Proteinosomes Supporting information for In Situ Gelation-Induced Death of Cancer Cells Based on Proteinosomes Yuting Zhou, Jianmin Song, Lei Wang*, Xuting Xue, Xiaoman Liu, Hui Xie*, and Xin Huang* MIIT Key Laboratory

More information

UNIVERSITY OF CAMBRIDGE INTERNATIONAL EXAMINATIONS General Certifi cate of Education Advanced Level

UNIVERSITY OF CAMBRIDGE INTERNATIONAL EXAMINATIONS General Certifi cate of Education Advanced Level UNIVERSITY F CAMBRIDGE INTERNATINAL EXAMINATINS General Certifi cate of Education Advanced Level *8308397844* CHEMISTRY 9701/41 Paper 4 Structured Questions May/June 2013 2 hours Candidates answer on the

More information

Version 1.1. Caspase-9 Fluorometric Assay Kit KC test

Version 1.1. Caspase-9 Fluorometric Assay Kit KC test Caspase-9 Fluorometric Assay Kit KC-04-012 100-200-500 test Index Introduction Pag. 1 Materials Pag. 2 Assay Principle Pag. 3 Reagent preparation Pag. 4 Assay protocol Pag. 5 Data analysis Pag. 6 Warranties

More information

Human cholecystokinin octapeptide, CCK-8 ELISA Kit

Human cholecystokinin octapeptide, CCK-8 ELISA Kit Human cholecystokinin octapeptide, CCK-8 ELISA Kit Catalog No: E1044h 96 Tests Operating instruction www.eiaab.com FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH

More information

CHIRAL SEPARATION USING THIN LAYER CHROMATOGRAPHY

CHIRAL SEPARATION USING THIN LAYER CHROMATOGRAPHY CHIRAL SEPARATION USING THIN LAYER CHROMATOGRAPHY Chiral Chromatography Chiral - adjective: not superimposable on its mirror image: used to describe a molecule whose arrangement of atoms is such that it

More information

Human antidiuretic hormone/vasopressin/arginine vasopressin, ADH/VP/AVP ELISA Kit

Human antidiuretic hormone/vasopressin/arginine vasopressin, ADH/VP/AVP ELISA Kit Human antidiuretic hormone/vasopressin/arginine vasopressin, ADH/VP/AVP ELISA Kit Catalog No: E1139h 96 Tests Operating instruction www.eiaab.cn FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC

More information

Human anti-deoxyribonuclease B, anti-dnase B ELISA Kit

Human anti-deoxyribonuclease B, anti-dnase B ELISA Kit Human anti-deoxyribonuclease B, anti-dnase B ELISA Kit Catalog No: E0311h 96 Tests Operating instruction www.eiaab.com FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ

More information

Investigating Nitroxide-Mediated Radical Polymerization of Styrene over a Range of Reaction Conditions

Investigating Nitroxide-Mediated Radical Polymerization of Styrene over a Range of Reaction Conditions Investigating Nitroxide-Mediated Radical Polymerization of Styrene over a Range of Reaction Conditions A. Nabifar N. T. McManus A. Penlidis Institute for Polymer Research (IPR) Department of Chemical Engineering

More information

Modification of Peptides, Oligonucleotides and Other Small Biomolecules with Tide Fluor (TF) Dye Succinimidyl Esters

Modification of Peptides, Oligonucleotides and Other Small Biomolecules with Tide Fluor (TF) Dye Succinimidyl Esters Modification of Peptides, Oligonucleotides and Other Small Biomolecules with Tide Fluor (TF) Dye Succinimidyl Esters Introduction The sulfonated cyanine dyes have improved fluorescence quantum yield than

More information

, for C 2. COOH is mol dm [1] COOH by adding water until the total volume is cm 3. for C 2 COOH.

, for C 2. COOH is mol dm [1] COOH by adding water until the total volume is cm 3. for C 2 COOH. 1 A student is supplied with 0.500 mol dm 3 potassium hydroxide, KOH, and 0.480 mol dm 3 propanoic acid, C 2 COOH. The acid dissociation constant, K a, for C 2 COOH is 1.35 10 5 mol dm 3. (a) C 2 COOH

More information

Click Chemistry Toolbox

Click Chemistry Toolbox Click Chemistry Toolbox Click Chemistry Biotinylation Regarents Fluorescent Click Chemistry Reagents Fluorescent Picolyl Azides Metabolic Labeling Reagents Cleavable Click Chemistry Biotin Probes Biotin/Streptavidin-Free

More information

Radical Polymerizations II Special Cases

Radical Polymerizations II Special Cases Radical Polymerizations II pecial Cases Devon A. hipp Department of Chemistry, & Center for Advanced Materials Processing Clarkson University Potsdam, NY 13699-5810 Tel. (315) 268-2393, Fax (315) 268-6610

More information

Lecture 2. Methods and Techniques for Self-assembly

Lecture 2. Methods and Techniques for Self-assembly 10.524 Lecture 2. Methods and Techniques for Self-assembly Instructor: Prof. Zhiyong Gu (Chemical Engineering & UML CHN/NCOE Nanomanufacturing Center) Lecture 2: Methods and Techniques for Self-assembly

More information

Preparation and Characterization of Organic/Inorganic Polymer Nanocomposites

Preparation and Characterization of Organic/Inorganic Polymer Nanocomposites Preparation and Characterization of rganic/inorganic Polymer Nanocomposites Proceedings of European Congress of Chemical Engineering (ECCE-6) Copenhagen, 16-20 September 2007 Preparation and Characterization

More information

Canine Erythropoietin,EPO ELISA Kit

Canine Erythropoietin,EPO ELISA Kit Canine Erythropoietin,EPO ELISA Kit Catalog No: E0028c 96 Tests Operating instructions www.eiaab.com FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH ENTIRE PROCEDURE

More information

PENNSYLVANIA. Explain the nature of the carbon atom and how organic molecules are important to organisms. Page 1 of B.A7.

PENNSYLVANIA. Explain the nature of the carbon atom and how organic molecules are important to organisms. Page 1 of B.A7. Know: Understand: Do: S11.C.1.1.1 -- Essential Explain that matter is made of particles called atoms and that atoms are composed of even smaller particles (e.g., proton, neutrons, electrons). S11.C.1.1.2

More information

MODELING AND PARAMETER ESTIMATION OF NITROXIDE MEDIATED LIVING FREE RADICAL POLYMERIZATION (NMRP) OF STYRENE

MODELING AND PARAMETER ESTIMATION OF NITROXIDE MEDIATED LIVING FREE RADICAL POLYMERIZATION (NMRP) OF STYRENE European Symposium on Computer Aided Process Engineering 15 L. Puigjaner and A. Espuña (Editors) 25 Elsevier Science B.V. All rights reserved. MODELING AND PARAMETER ESTIMATION OF NITROXIDE MEDIATED LIVING

More information

Recommended Procedures for Labeling. Labeling Proteins with Amine-Reactive ATTO-Labels (NHS-Esters) Introduction

Recommended Procedures for Labeling. Labeling Proteins with Amine-Reactive ATTO-Labels (NHS-Esters) Introduction Recommended Procedures for Labeling Introduction ATTO-TEC offers a large variety of high-quality dyes for labeling amino and thiol groups. ATTO reactive dyes cover the spectral region from 350 nm in the

More information

Tuesday 13 June 2017 Afternoon Time allowed: 2 hours 15 minutes

Tuesday 13 June 2017 Afternoon Time allowed: 2 hours 15 minutes Oxford Cambridge and RSA A Level Chemistry A H432/01 Periodic table, elements and physical chemistry *6825819547* Tuesday 13 June 2017 Afternoon Time allowed: 2 hours 15 minutes You must have: the Data

More information

SURFACE COVALENT ENCAPSULATION OF MULTI-WALLED CARBON NANOTUBES BY POLYMER GRAFT

SURFACE COVALENT ENCAPSULATION OF MULTI-WALLED CARBON NANOTUBES BY POLYMER GRAFT SURFACE COVALENT ENCAPSULATION OF MULTI-WALLED CARBON NANOTUBES BY POLYMER GRAFT Yanxin Liu, Zhongjie Du, Yan Li, Chen Zhang, Xiaoping Yang, Hangquan Li, The Key Laboratory of Beijing City on Preparation

More information

Supplementary Information

Supplementary Information Supplementary Information Supplementary Figure 1 Raman spectroscopy of CVD graphene on SiO 2 /Si substrate. Integrated Raman intensity maps of D, G, 2D peaks, scanned across the same graphene area. Scale

More information

Supplementary Information Effects of asymmetric nanostructures on the extinction. difference properties of actin biomolecules and filaments

Supplementary Information Effects of asymmetric nanostructures on the extinction. difference properties of actin biomolecules and filaments Supplementary Information Effects of asymmetric nanostructures on the extinction difference properties of actin biomolecules and filaments 1 E. H. Khoo, 2 Eunice S. P. Leong, 1 W. K. Phua, 2 S. J. Wu,

More information

CHEM J-8 June Complete the following table. Make sure you give the name of the starting material where indicated. REAGENTS/ CONDITIONS

CHEM J-8 June Complete the following table. Make sure you give the name of the starting material where indicated. REAGENTS/ CONDITIONS CEM1102 2014-J-8 June 2014 Complete the following table. Make sure you give the name of the starting material where indicated. STARTIG MATERIAL REAGETS/ CDITIS STRUCTURAL FRMULA(S) F MAJR RGAIC PRDUCT(S)

More information

Reconfigurable DNA Origami Nanocapsule for ph- Controlled Encapsulation and Display of Cargo

Reconfigurable DNA Origami Nanocapsule for ph- Controlled Encapsulation and Display of Cargo Supporting Information Reconfigurable DNA Origami Nanocapsule for ph- Controlled Encapsulation and Display of Cargo Heini Ijäs, Iiris Hakaste, Boxuan Shen, Mauri A. Kostiainen, and Veikko Linko* Biohybrid

More information

According to the manufacture s direction (Pierce), RNA and DNA

According to the manufacture s direction (Pierce), RNA and DNA Supplementary method Electrophoretic Mobility-shift assay (EMSA) According to the manufacture s direction (Pierce), RNA and DNA oligonuleotides were firstly labeled by biotin. TAVb (1pM) was incubated

More information

Supporting Information for:

Supporting Information for: Supporting Information for: Preparation and Cross-linking of All-Acrylamide Diblock Copolymer Nano-objects via Polymerization-Induced Self-Assembly in Aqueous Solution Sarah J. Byard, Mark Williams, Beulah

More information

Supplementary Information

Supplementary Information Supplementary Information 1. Experimental 1.1.1 Synthesis of hollow silica nanoparticles NPs. The precursor of sol-gel silica is fresh made 1.0 M Si(OH) 4 solution which was made by adding tetramethyl

More information

Supporting Information: Plasmonic hepatitis B biosensor for the analysis of clinical saliva

Supporting Information: Plasmonic hepatitis B biosensor for the analysis of clinical saliva Supporting Information: Plasmonic hepatitis B biosensor for the analysis of clinical saliva Tomáš Riedel, *, Simone Hageneder, František Surman, Ognen Pop-Georgievski, Christa Noehammer, Manuela Hofner,

More information

Lys-HRP Protein Conjugation Kit

Lys-HRP Protein Conjugation Kit LysHRP Protein Conjugation Kit Description LysHRP Protein Conjugation Kits provide sufficient reagents to perform two antibodyhrp conjugation reactions. Any suitable amount of antibody (100500 µg) in a

More information

Manufacturing of Anisotropic Particles by Site Specific Oxidation of Thiols

Manufacturing of Anisotropic Particles by Site Specific Oxidation of Thiols Manufacturing of Anisotropic Particles by Site Specific Oxidation of Thiols Kristofer Eriksson, LarsErik Johansson, Emmanuelle Göthelid, Leif Nyholm and Sven Oscarsson Supporting Information Experimental.

More information

Biophysics of Macromolecules

Biophysics of Macromolecules Biophysics of Macromolecules Lecture 11: Dynamic Force Spectroscopy Rädler/Lipfert SS 2014 - Forced Ligand-Receptor Unbinding - Bell-Evans Theory 22. Mai. 2014 AFM experiments with single molecules custom-built

More information

Phys 450 Spring 2011 Solution set 6. A bimolecular reaction in which A and B combine to form the product P may be written as:

Phys 450 Spring 2011 Solution set 6. A bimolecular reaction in which A and B combine to form the product P may be written as: Problem Phys 45 Spring Solution set 6 A bimolecular reaction in which A and combine to form the product P may be written as: k d A + A P k d k a where k d is a diffusion-limited, bimolecular rate constant

More information

ORANGE Peroxidase Substrate Kit

ORANGE Peroxidase Substrate Kit ORANGE Peroxidase Substrate Kit Catalog Number KA0563 1000 Slides Version: 02 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use...3 Principle of the Assay...3

More information

Effect of Molecular Structure of Side Chain Polymers on "Click" Synthesis of Thermosensitive Molecular Brushes

Effect of Molecular Structure of Side Chain Polymers on Click Synthesis of Thermosensitive Molecular Brushes University of Tennessee, Knoxville Trace: Tennessee Research and Creative Exchange University of Tennessee Honors Thesis Projects University of Tennessee Honors Program 5-2017 Effect of Molecular Structure

More information

Chiral Surface of Nanoparticles Determines the Orientation of Adsorbed Transferrin and its Interaction with Receptors

Chiral Surface of Nanoparticles Determines the Orientation of Adsorbed Transferrin and its Interaction with Receptors Supporting information Chiral Surface of Nanoparticles Determines the Orientation of Adsorbed Transferrin and its Interaction with Receptors Xinyi Wang,,,# Mingzhe Wang,,# Rong Lei, Shui fang Zhu, Yuliang

More information