CHEM 460 / 560 Prebiotic Chemistry
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1 CHEM 460 / 560 Prebiotic Chemistry Dr. Niles Lehman Department of Chemistry Portland State University niles@pdx.edu Chem_460_560.html
2 The Timeline of Life Joyce (2002) Nature 418,
3 timelines: ORIGINS OF LIFE ON THE EARTH 15 bya 10 bya 5 bya 0 bya BIG BANG (see P&G Fig. 2.1) origin of the sun origin of the Earth eubacteria/ archaea split origins of multicellularity
4 What is Life? the list of characteristics approach: growth response to stimulus metabolism reproduction evolution LIFE = a self-sustaining chemical system capable of darwinian evolution (Joyce/NASA)
5 life
6 non-life
7 the non-life-to-life transition at 4.0 +/ 0.1 billion years ago a dead bag of chemicals??? an alive bag of chemicals Lehman: the origins of life is a chemical problem in a biological context
8 autocatalysis A + B C autocatalysis is a situation in which the product of a reaction catalyzes its own synthesis from reactants add Mn ++ 2MnO H2C2O4 + 6H30 + 2Mn CO2 + 14H2O a necessary, but not sufficient, requirement for life
9 the chemistry of life the life on the Earth is based on Carbon C atomic number = 6 electronic configuration: 1s 2, 2s 2, 2p 2 atomic mass = isotopic abundance on Earth: 11 C = 0% (synthetic) 12 C = 98.9% 13 C = 1.1% 14 C = 1 PPT ( %)
10 carbon vs. silicon carbon is more suitable for life (self-reproducing and evolving systems) because: the C-H, C-N, C-O, and C-C bond energies are similar C-X single, double, and triple bond energies are similar breaking of the C-H bond requires high ΔEa carbon dioxide, the oxidative end product, is a gas
11 the stuff of life proteins (amino acids) lipids (alcohols & fatty acids) carbohydrates (sugars) nucleic acids (nucleotides) small molecules (water, metals, ions, etc.) all are polymers formed by condensation reactions...in the primordial soup?
12 the elements of life sum = about 22 elements
13 elemental abundances in the universe for our Sun: see P&G, Fig. 1.2; for rocky planets, see P&G, Fig. 1.3
14 water is the solvent of life water is highest water is high water is lowest
15 the three stages in the evolution of life 1. chemical evolution 2. self-organization 3. biological evolution
16 life can be considered a negentropy machine hν ΔS < 0 heat 1. Light energy from the sun is absorbed by the Earth and eventually converted into energy that living things can use (ATP). 2.Living thing use this energy and perhaps convert it to other forms of chemical energy, but this conversion is not perfect...some is lost as low-grade energy (heat). 3.Life then, uses the sun s energy to maintain its own order. 4.Because the environment is constantly changing, life must acquire information from the environment (through sensing devices) and alter its own information content accordingly. 5.Life, therefore, are little pockets of NEGENTROPY, where the order is temporarily greater than its surroundings.
17 The Seven Challenges to a Prebiotic Chemist 1. The origin/source of the elements 2. The origin/source of small molecule precursors 3. The origin/source of monomers 4. The condensation problem 5. The self-replication problem 6. The chirality problem 7. The compartmentalization problem
18 The Seven Challenges to a Prebiotic Chemist 1. The origin/source of the elements 2. The origin/source of small molecule precursors 3. The origin/source of monomers 4. The condensation problem 5. The self-replication problem 6. The chirality problem 7. The compartmentalization problem
19 The Big Bang 13.7 bya
20 the four fundamental forces in Nature strong nuclear force weak nuclear force >> >> >> electromagnetic force gravitational force holds nuclear particles together (p + n) responsible for radioactive decay (n p + e ) holds electrons to nuclei (CHEMISTRY) holds matter together into larger structures
21 from the Big Bang to the formation of our Solar system t = 0 : the Big Bang -- only electrons, neutrons, protons, and photons e, n, p, hν
22 from the Big Bang to the formation of our Solar system t = 100 sec : temperature cooled below 1 billion K; the strong nuclear force was no longer overwhelmed, and protons and neutrons could combine to form nuclei Big Bang nucleosynthesis p = 1 H p + p D D + p 3 He 3 He + 3 He 4 He + 2p
23 from the Big Bang to the formation of our Solar system t = 377,000 years: temperature cooled below 3000 K; the recombination era the electromagnetic force was no longer overwhelmed, and electrons could remain with nuclei
24 universe anisotropy was key to life! the background microwave radiation in the universe is slightly anisotropic: it does NOT look exactly the same in all directions
25 universe anisotropy was key to life! 10-parts-per-million differences in energetic distributions led to... unequal mass distributions, which led to... clumping of interstellar gasses, which led to... a trillion or so lumps of protogalaxies, inside of which other anisotropies led to... STAR SYSTEM FORMATION
26 (formation of stars = elements, the solar accretion system, & the Earth) protostar accretion discs protoplanets inner, rocky planets outer, gaseous planets
27 nucleosynthesis in the Sun Sun: T = 16 million K the Bethe & Weizsacker carbon cycle
28 distribution of heavier elements via supernovae events elements above atomic number 26 (Fe) come from exploding stars elsewhere
29 planetary formation inner, rocky planets: Cn, Sin, Fe outer, gaseous planets: H2, He, NH3, and CH4
30 formation of Earth s moon massive collision at / 0.01 bya was another key event in the origins of life
31 the history of large impacts on the Earth and Moon moon-formation impact red: impacts on Moon blue: impacts on the Earth
32 habitable zones solar system habitable zone only one star our Sun is relatively massive broad region where liquid water can form Earth is outside tidal lock zone Earth has a moon Jupiter is out there galactic habitable zone not too near the galactic center not too far away from the galactic center the Sun s orbit is circular
33 The Seven Challenges to a Prebiotic Chemist 1. The origin/source of the elements 2. The origin/source of small molecule precursors 3. The origin/source of monomers 4. The condensation problem 5. The self-replication problem 6. The chirality problem 7. The compartmentalization problem
34 the central dogma of molecular biology Figure 5-21 The central dogma of molecular biology. life: needs all this plus anything else to keep it safe
35 the chemical requirements of Life proteins (amino acids) lipids (alcohols & fatty acids) carbohydrates (sugars) nucleic acids (nucleotides) small molecules (water, metals, ions, etc.) all are polymers formed by condensation reactions...in the primordial soup?
36 review: elements of life nucleic acids (CHOPN) proteins (CHOSN) lipids (CHO) polysaccharides (CHO) catalysts (Fe, Mg, Ca, Mn, Ni, Zn, Cu, Se, Co, Mo) counterions (Na, K, F, Cl, Br, I) neutrals, for clays (Al, Si) in total, about elements: H, C, N, O, F, Na, Mg, Al, Si, P, S, Cl, K, Ca, Mn, Fe, Co, Ni, Cu, Zn, Se, Br, Mo, I
37 Darwin s Warm Little Pond It is often said that all the conditions for the first production of a living organism are now present, which could ever be present. But if (and oh! what a big if) we could conceive in some warm little pond with all sorts of ammonia and phosphoric salts, light, heat, electricity, etc., present, that a protein compound was chemically formed ready to undergo still more complex changes, at the present day, such matter would be instantly devoured or absorbed, which could not have been the case before living creatures were formed. Darwin, 1871, unpublished letter
38 small molecules in interstellar space, as detected by radiotelescopy hydrogen cyanide formaldehyde > 120 organic molecules have been detected to date, mostly by microwave spectroscopy (Benner, 2009) acetaldehyde glycoaldehyde
39 relative abundances of molecules in space
40 Small Molecule Precursors Found in space: hydrogen cyanide (HCN) acetlyene (HC CH) formic acid (HCOOH) formaldehyde (H2CO) acetic acid (CH3COOH) ammonia (NH3) water Found in comets & meteorites: amino acids nucleobases lipids PAHs water abundant on early Earth: hydrogen sulfide, CO, water, methane, salts, etc.... but how?
41 the Earth s early atmosphere once the Earth accreted, it formed a primary atmosphere but it was soon able to evolve its own, secondary atmosphere through outgassing of its interior in particular, the outgassing of H 2 occurred gradually but steadily (contemporary atmospheres of Venus, Earth, and Mars: Zubay Table 5-2)
42 contemporary atmospheres of Venus, Earth, and Mars
43 the Earth s early atmosphere three important molecules could then form in the early atmosphere: 1. water vapor (H2O) * 2. methane (CH4) 3. ammonia (NH3) other gasses probably present: CO & N 2, plus those that are currently outgassing: CO2, HCl, and H2S
44 the Earth s early atmosphere the big question: oxidizing (e poor) = BAD vs. reducing (e rich) = GOOD the dominant view recently (e.g., Jim Kasting) has been that the primitive atmosphere was a weakly reducing mixture of CO2, N2, and H2O, combined with lesser amounts of CO and H2
45 the Earth s early atmosphere the big question: oxidizing vs. reducing any O2 made abiotically could have been lost from the atmosphere by reactions with: H2 (to give water) CO (to give carbonate) Si (to give silicates = glass) Fe(II) to give Fe(III) 4Fe(II)O + O2 2Fe2(III)O3 banded iron
46 four key reactions could have occurred in this type of atmosphere: 1. CO2 + 2H2 H2CO + H2O abiotic formaldehyde 2. N2 + hν 2N nitrogen photolysis 3. CO2 + 2H2O CH4 + 2O2 abiotic methane 4. 2CH4 + 2N + hν 2HCN + 3H2 abiotic hydrogen cyanide H2CO and HCN were major players in future reactions!!!
47 again, the OoL timing (4.0 +/- 0.1 bya) is bounded by two events: more recent boundary: oldest BIF dates to 3.85 bya more ancient boundary: severe meteoritic impacts still occurring once per 50,000 years at 4.2 bya
48 some sources of small molecule precursors: H2, N2, CO, CH4, etc. molecular hydrogen (H2) is not common in life, but may have been critical in the OoL for its roles in the formation of water and simple hydrocarbons gasses such as N2 and CO were very important, because they were the ultimate sources of nitrogen and reducible carbon, respectively hydrogen cyanide (HCN), acetylene (HCCH), and formaldehyde (H2C=O) are abundant in interstellar gasses; these molecules can provide reducing power (e ) for the OoL
49 some sources of small molecule precursors: water water is the solvent of life today, 2/3 of the Earth s surface is water water could have been abundant in significant (to the OoL) amounts on the early Earth as soon as 4.3 bya (Steve Mojzsis) water can be formed by the reduction of oxygen-containing compounds such as CO, but only at high temperatures or pressures, so this likely happened during the original accretion of the Earth after the Earth was formed, water was probably delivered by comets that impacted the Earth most of the Earth s water likely had an extraterrestrial origin in space: 1. 3O2 + UV > 2O3 2. O3 + 3H2 > 3H2O
50 the influence of the Solar System s Big Brother Jupiter some of the volatiles on the early Earth were there because of the gaseous planets, Neptune, Uranus, Saturn, and particularly Jupiter the massive gravity of this planet helped to clean up the protoplanetary debris in the Solar System the debris either got ejected from the Solar System or condensed into the inner planets, where they could be delivered to Earth via meteorites carbonaceous chondrites: rich in carbon, 3% total organics, and 5% water
51 with these few molecules, plus gasses, the larger components of life must have been made possible sources of energy for the OoL
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53 The Seven Challenges to a Prebiotic Chemist 1. The origin/source of the elements 2. The origin/source of small molecule precursors 3. The origin/source of monomers 4. The condensation problem 5. The self-replication problem 6. The chirality problem 7. The compartmentalization problem
54 comets a comet is a small, icy Solar System body
55 Darwin s Warm Little Pond the primordial soup = the primordial ooze
56 monomers amino acids O NH 2 OH N NH 2 N O N N fatty acids O OH H OH - O P O - O O H H H H OH OH nucleotides HO HO H H H O OH OH sugars H
57 the source of monomers - e.g., amino acids H2 + NH3 + CH4 + H2O energy H2N CH2 COOH a dead bag of chemicals glycine, an amino acid
58 early theories on the origins of life from a chemical evolution perspective Darwin e.g., 1871 JBS Haldane ( ) Alexandr Ivanovich Oparin ( ) a dead bag of chemicals other, more complex chemicals
59 JBS Haldane (British Geneticist) Haldane thought much about prebiotic chemistry, but, as a geneticist, did few actual experiments on the topic In 1923, gave a talk at Cambridge on the possibility of hydrogen-generating windmills as an alternative to coal fuel In 1925, developed the Briggs Haldane derivation of the Michaelis-Menten enzyme kinetic equation In 1929, wrote an article for the Rationalist Annual called The Origin of Life may have coined the phrase prebiotic soup
60 JBS Haldane The Earth s earliest atmosphere would have been devoid of molecular oxygen, and rather, comprised of ammonia and carbon dioxide. Without O2, there would be no O3 to protect the Earth from ultraviolet radiation, which could have provided energy for the polymerization of small molecules into proteins
61 Alexandr Ivanovich Oparin (Russian Biochemist) Oparin postulated a long chemical evolution as a necessary preamble to the emergence of life He devised a sequence of plausible reactions, and then actually did some experimentation to test his ideas Oparin Was perhaps the first to seriously consider the abiotic origins of cell-like structures
62 Oparin Wrote a seminal book on the topic in Russian in 1924 He was really the first to consider the incoming data on the formation and composition of the Sun and the planets In the early 1930 s it was possible to study the Sun s elemental make-up and to observe the atmospheric compositions of nearby planets, especially Venus English edition, first published in 1938
63 Oparin s Chemical Evolution His first conclusion: carbon made its first appearance on the Earth not in the oxidized form of CO2 but in the reduced form of hydrocarbons He believed the Earth s earliest atmosphere was strongly reducing Was influenced by experiments of other Russians that showed that iron carbides could react with hot water to generate hydrocarbons: 3FemCn + 4mH2O mfe3o4 + C3nH8m e.g., m = n = 1: 3FeC + 4H2O Fe3O4 + C3H8 iron in reduced state (Fe(II)) is converted to a mixed oxidation state during the reduction of carbide to propane
64 Oparin s ideas on the early atmosphere Was concerned about the source of nitrogen, because of its important role in proteins He didn t think the early atmosphere contained much O2 or N2 Thus he proposed that nitrogen first became trapped in the Earth s core at high temperatures by the formation of metal nitrides, then released as ammonia upon oxidation by water vapor: Δ Δ Δ 1. 3Mg + N2 Mg3N2; 2Al + N2 Al2N2; 2Fe + N2 2FeN 2. FeN + 3H2O Fe(OH)3 + NH3 another possibility: the Haber production of ammonia, occurring in the upper portions of the Earth s crust
65 Oparin s pathway from simple hydrocarbons to more complex biologically relevant molecules aldehydes (e.g., acetaldehyde) could have been produced by the hydration of acetylene: CH CH + H2O CH3CHO two acetaldehyde molecules could have condensed by an aldol condensation reaction to give an alcohol: 2CH3CHO CH3CHOHCH2CH2OH a succession of such condensations could have led to glucose, a polyol:
66 the aldol condensation reaction two aldehydes condense to form a more complex alcohol: 1. tautomerization of an aldehyde to an enol or enolate (base catalyzed) 2. nucleophilic attack of the enol on the carbonyl center of another aldehyde to give an addition product 3. re-protonation to give the β-hydroxy aldehyde Geoffrey Zubay: The synthesis of sugars in the prebiotic world is likely to have started with formaldehyde
67 the aldol condensation reaction later, we will see the importance of this type of process in driving the formose reaction nch2o (CH2O)n {the fixation of formaldehyde into carbohydrates}
68 Oparin s realized the problem of concentrations! prebiotic chemistry has an intrinsic problem in that a series of reactions with <100% yields mandates lower and lower probabilities of products with each additional step if each step occurs in low yield, or if the concentrations of precursors is low, then the overall yield is in danger of being so small as to be negligible the high concentrations of water on the early Earth would have diluted reactants, diffused away products, AND inhibited condensation reactions Oparin proposed that simple cell-like structures called coacervates were needed at or near the origins of life to deal with these issues
69 Oparin s coacervates μm in diameter Coacervates, which are polymer-rich collodial droplets, were studied in the Moscow laboratory of Oparin because of their conjectural resemblance to prebiological entities. These coacervates are droplets formed in an aqueous solution of protamine and polyadenylic acid. Oparin found that droplets survive longer if they can carry out polymerization reactions inside.
70 The Seven Challenges to a Prebiotic Chemist 1. The origin/source of the elements 2. The origin/source of small molecule precursors 3. The origin/source of monomers 4. The condensation problem 5. The self-replication problem 6. The chirality problem 7. The compartmentalization problem
71 the source of monomers - amino acids H2 + NH3 + CH4 + H2O energy H2N CH2 COOH a dead bag of chemicals glycine, an amino acid The Miller-Urey spark-discharge experiments
72 the source of monomers - amino acids glycine, alanine, aspartic acid, etc. the Miller-Urey spark-discharge experiments ( )
73 The original Miller-Urey Experiment (1952) CH4 (20 torr) + NH3 (20 torr) + H2 (10 torr) + H2O (vapor) 500 ml flask: water ( ocean ) + 2 L flask: gas ( atmosphere ) 2000 V spark; one-week incubation time Miller (1953) Science 111:
74 The original Miller-Urey Experiment (1952) CH4 + NH3 + H2O + H2 + energy : paper chromatography glycine > α-alanine > α-amino-n-butyric acid > β-alanine > glutamic acid > aspartic acid
75 Results from the original Miller-Urey Experiment (1952) overall, about 15% of the carbon in methane is converted to intermediate-sized molecules by this technique = Table 4.2 in P&G
76 subsequent Miller-Urey experiments (1953 ) varied the input gasses & concentrations all the way from strongly reducing (best yields) to mildly oxidizing (poorer yields) varied flask configurations and gas pressure varied energy source (e vs. UV vs. heat, etc.) & time
77 subsequent Miller-Urey experiments (1953 ) proteinaceaous amino acids, their isomers, and other amino acids that are formed; total AA yield = 1.90% = Table 4.3 in P&G
78 intermediates in Miller-Urey experiments the appearance and then disappearance of HCN and aldehydes reveals that they are key intermediates = P&G Fig V: produces free radicals to drive production of intermediates
79 H variant Strecker synthesis of amino acids and hydroxy acids 0. the production of aldehydes and HCN via free-radical chemistry from simple gaseous starting materials, for example: a) CH4 + H2O H2CO + H2 [CH4 + e * CH3 + H + ] b) 2CH4 + N2 2HCN + 3H2 [N2 + e * 2N ] 1. the production of a cyanoamine: RCH=O + NH3 + HC N 2. the hydration of the cyanoamine to give an amino acid: RCHNH2C N NH2 RCHNH2C N +2H2O R C COOH
80 the classic Strecker synthesis of amino acids
81 the Strecker synthesis of amino acids and hydroxy acids 1. the addition of ammonia to an aldehyde to give an imine: 2. the addition of cyanide to the imine to give a cyanoamine (aminonitrile): 3. hydrolysis of the cyanoamine to give an amino acid: 2 & 3. the addition of cyanide to the aldehyde directly and then hydrolysis gives a hydroxy acid instead: = Fig. 4.5 in P&G
82 cyano compounds of prebiological interest HC N (hydrogen cyanide): basic precursor to almost all biological monomers; formed from CH4 and NH3 N C NH2 (cyanamide): activator for peptide condensation N C C CH (cyanoacetylene): formed from CH4 and N2; used in pyrimidine abiosynthesis; used in Asp and Asn abiosynthesis N C CH=NH (iminoacetonitrile): HCN dimer; used in purine abiosynthesis R CH2(NH2) C N (aminonitriles) & R CH2(OH) C (hyrdoxynitriles): used in amino acid abiosynthesis N
83 the Strecker synthesis should produce a racemic mixture amino acids found in the Miller experiments are indeed racemic; amino acids found in meteorites have some ee; amino acids in proteins are all L
84 Collision in the asteroid belt! Potential meteorites! courtesy of Dave Deamer
85 courtesy of Dave Deamer
86 September 28, 1969 Murchison, Australia courtesy of Dave Deamer 5!
87 the amino acids in the Miller-Urey syntheses match those found in meteorites (such as the Murchison) rather well meteorites contain detectable amounts of many amino acids, especially glycine, alanine, and α- amino-n-butyric acid, along with a range of hydroxy acids
88 the Miller-Urey experiments have produced at least 17 of the 20 or so proteinaceaous amino acids some require subsequent modifications the three aromatics, Tyr, Trp, and Phe require an alternative synthetic route
89 Miller has proposed an abiotic route to histidine that mimics the biosynthetic route erythrose would come from the formose reaction (coming soon!)
90 Miller s experiment generated instant media attention Milk, meat, albumen, bacteria, viruses, lungs, hearts all are proteins. Wherever there is life there is protein stated the New York Times in its May 15, 1953 issue. Protein is of fairly recent origin, considering the hot state of the earth in the beginning. How the proteins and therefore life originated has puzzled biologists and chemists for generations. Accepting the speculations of the Russian scientist A. I. Oparin of the Soviet Academy of Science, Prof. Harold C. Urey assumes that in its early days the earth had an atmosphere of methane (marsh gas), ammonia and water. Oparin suggested highly complex but plausible mechanisms for the synthesis of protein and hence of life from such compounds. In a communication which he publishes in Science, one of Professor Urey s students, Stanley L. Miller, describes how he tested this hypothesis, continued the New York Times, A laboratory earth was created. It did not in the least resemble the pristine earth of two or three billion years ago; for it was made of glass. Water boiled in a flask so that the steam mixed with Oparin s gases. This atmosphere was electrified by what engineers call a corona discharge. Miller hoped that in this way he would cause the gases in his artificial atmosphere to form compounds that might be precursors of amino acids, these amino acids being the bricks out of which multifarious kinds of protein are built. He actually synthesized some amino acids and thus made chemical history by taking the first step that may lead a century or so hence to the creation of something chemically like beefsteak or white of egg. Miller is elated, and so is Professor Urey, his mentor.
91 The Seven Challenges to a Prebiotic Chemist 1. The origin/source of the elements 2. The origin/source of small molecule precursors 3. The origin/source of monomers 4. The condensation problem 5. The self-replication problem 6. The chirality problem 7. The compartmentalization problem
92 cyano compounds of prebiological interest HC N (hydrogen cyanide): basic precursor to almost all biological monomers; formed from CH4 and NH3 N C NH2 (cyanamide): activator for peptide condensation N C C CH (cyanoacetylene): formed from CH4 and N2; used in pyrimidine abiosynthesis; used in Asp and Asn abiosynthesis N C CH=NH (iminoacetonitrile): HCN dimer; used in purine abiosynthesis R CH2(NH2) C N (aminonitriles) & R CH2(OH) C (hyrdoxynitriles): used in amino acid abiosynthesis N
93 The RNA World a proposed period of time when RNA (or something like RNA) was responsible for all metabolic and information-transmission processes RNA has both a genotype AND a phenotype (Cech, Altman: catalytic RNA... Nobel Prize, 1989) Catalytic RNA = ribozymes (9 classes) The ribosome is a ribozyme
94 The RNA World......needs ribose, nucleobases, and phosphates
95 The Source of Monomers - ribose sugars HO O OH OH OH ribose requires 5 carbons, C-O bonds, and correct stereochemistry two acetaldehyde molecules could have condensed by an aldol condensation reaction to give an alcohol: 2CH3CHO CH3CHOHCH2CH2OH a succession of such condensations could have led to glucose, a polyol:
96 The Source of Monomers - ribose sugars glycoaldehyde formaldehyde DL-glyceraldehyde ribose The formose reaction (autocatalytic)
97 the formose reaction Butlerov (1860): formaldehyde + water + calcium hydroxide + heat gives a mixture of sugars HO O OH OH OH formaldehyde is used to make glycoaldehyde, trioses, and tetroses; pentoses such as ribose are made by the condensation of glycoaldehyde and a triose
98 the formose reaction optimal: high ph, calcium hydroxide, 55 C, 1-2% aqueous formaldehyde The formose reaction exploits the natural nucleophilicity of the enediolate of glycoaldehyde and the natural electrophilicity of formaldehyde. The calcium ion stabilizes the enediolate of glycoladehdye. This species reacts as a nucleophile with formaldehyde (acting as an electrophile) to give glyceraldehyde. Reaction of glyceraldehyde with a 2nd equivalent of the enediolate generates a pentose sugar (ribose, arabinose, xylose, or lyxose)
99 The formose reaction is autocatalytic DL-glyceraldehyde glycoaldehyde tetrose glycoaldehyde is the autocatalytic reagent: it is both the product of the condensing of two formaledhyde molecules AND a catalyst for this condensation
100 The formose reaction is autocatalytic C3: DL-glyceraldehyde C2: glycoaldehyde glycoaldehyde is the autocatalytic reagent: it is both the product of the condensing of two formaledhyde molecules AND a catalyst for this condensation
101 The formose reaction is autocatalytic glycoaldehyde the glycoaldehyde cycle = Fig. 4.7 P&G
102 ribose is but one of many possible 5-carbon sugars: 3C 4C 5C 6C
103 then the straight-chain form must cyclize: (6C example)
104 The formose reaction produces a dizzying array of products ribose Decker, Schweer, & Pohlmann (1982) J. Chromatogr. 244: GC
105 The formose reaction can make ribose, but the yield is poor (<1%) and MANY other products arise Possible solutions: phosphorylating the glycoaldehyde (Eschenmoser, 1990) using lead salts and mildly basic conditions (Zubay, 1998) boron complexation (Benner, 2004) membranes can be selectively permable (Szostak, 2005) silicate complexes (Lambert, 2010) alternative backbones: PNA, TNA, etc.
106 Albert Eschenmoser: use phosphate! Using phosphorylated glycoaldehyde not only give you phosphorylated sugars, but it also greatly biases products towards ribose:
107 Geoff Zubay: use lead! Lead (II) ions can increase the yields of aldopentoses from formaldehyde by over 20-fold Zubay, 1998 the power of lead (II) is a result of its high affinity for cis-hydroxyls and its very low pka value (the pka of hydrated lead (II) ions is about 7.7)
108 Steve Benner: use borate! Borate ions can stabilize glyceraldehydes, preventing them from acting as nucleophiles and thus stemming out-of-control polymerization HO H H H O ulexite NaCaB5O9 8H2O HO O O B O OH O O glycoaldehyde + DL-glyceraldehyde Ca(OH)12 boron mineral pentoses as majority Ricardo, Carrigan, Olcott, & Benner (2004) Science 303, 196
109 Jack Szosak: use cell membranes! Sacerdote and Szostak (2005). Proc. Natl. Acad. Sci. USA,102: using certain phospholipid membranes in artificial cells results in a greatly increased permeability to ribose vs. other pentoses and sugars
110 Joseph Lambert: use silicates! aqueous sodium silicate can select for sugars with a specific stereochemistry Lambert et al. (2010). Science,327:
111 maybe ribose came later, and simpler backbones came first: GNA: glycerol-derived acyclonucleic acid TNA: threose nucleic acid p-rna: pyranose RNA
112 maybe ribose came later, and simpler backbones came first: TNA PNA: peptide nucleic acid GNA p-rna Joyce (2004)
113 The Seven Challenges to a Prebiotic Chemist 1. The origin/source of the elements 2. The origin/source of small molecule precursors 3. The origin/source of monomers 4. The condensation problem 5. The self-replication problem 6. The chirality problem 7. The compartmentalization problem
114 The RNA World......needs ribose AND nucleobases, AND phosphates
115 conventional wisdom: 1a. make nucleobase 1b. make ribose (e.g., formose rxn) 1c. find phosphate source 2. add base to sugar 3. add phosphate
116 the source of monomers - nucleobases NH 2 hydrogen cyanide (HCN) recombination 5 H C N H N N H N N H adenine read P&G s discussion of HCN on the Earth (pp ) 15 atoms & 50 electrons: 5 C-H bonds 5 C-N bonds present in interstellar medium 15 atoms & 50 electrons: 2 C-H bonds 9 C-N bonds 3 N-H bonds 1 C-C bond present in living systems the Oró HCN polymerization experiments (1961-)
117 the mechanism of Oró HCN polymerization HCN 1. dimerization of HCN 2. trimerization to aminomaleonitrile 3. tetramerization to DAMN 4. UV-induced isomerization 5. final HCN addition and ring closure adenine We come from stardust and stardust we will become. We must be humble, because life comes from very simple molecules. We must be supportive, because we have a common origin. We have to be cooperative, since from the Moon the Earth is seen as a speck lost in the vastness of space, where the boundaries between people and the color of their skin cannot be distinguished. Joan Oró (1976)
118 the mechanism of Oró HCN polymerization optimum rate at ph 9.2 (pka of HCN) iminoacetonitrile = P&G Fig dimerization of HCN 2. trimerization to aminomaleonitrile 3. tetramerization to DAMN 4. UV-induced isomerization 5. final HCN addition and ring closure
119 the mechanism of Oró HCN polymerization = P&G Fig dimerization of HCN 2. trimerization to aminomaleonitrile 3. tetramerization to DAMN 4. UV-induced isomerization 5. final HCN addition and ring closure
120 the mechanism of Oró HCN polymerization 1. dimerization of HCN 2. trimerization to aminomaleonitrile 3. tetramerization to DAMN 4. UV-induced isomerization 5. final HCN addition and ring closure
121 Zubay: last HCN addition may come after a formylation instead, akin to purine biosynthesis
122 Adenine Guanine AICA equivalent biosynthesis of purines
123 HCN polymerization (courtesy of Tim Riley)
124 other purines
125 pyrimidines -- more difficult
126 Various pyrimidines can be formed using UV light in ammonia-rich ices Nuevo et al. (2012) Astrobiology 12:
127 attaching base to sugar... O N NH O N N - O P O - O H O H H OH H OH IMP Leslie Orgel: hypoxanthine + D-ribose + Mg 2+ gives β-inosine under dehydrating conditions (low yield) this reaction does not work for the pyrimidines!
128 The Source of Monomers - phosphates Possible sources of phosphates: fluorapatite in Earth s crust: Ca10(PO4)6F2 schreibersite in iron meteorites: (Fe, Ni)3P alkyl phosphonic acids in meteorites: R H2PO3 Nearly all phosphorus in the Earth s crust is in the form of orthophosphate, which has low reactivity toward organic compounds, and thus phosphate minerals are not good bets for the abiotic P source.
129 phosphorus compounds
130 phosphates from more reduced forms of P schreibersite is a rare iron-nickel phosphide mineral, but is common in iron-nickel meteorites There is evidence that schreibersite, when dissolved in water, can form pyrophosphate, which can phosphorylate sugars (Matt Pasek, U. Arizona)
131 evolution of molecular hydrogen after soaking of Fe3P in water, indicating the production of phosphates Pasek & Lauretta (2005) Astrobiology 5:
132 The Source of Monomers - making a complete nucleotide RNA-catalyzed nucleotide assembly? Joyce (2002) example: nucleotide synthetase ribozyme Unrau & Bartel (1998) Nature 395,
133 The Source of Monomers - making a complete nucleotide A difficult task! Could RNA have been a biotic invention? {Anastasi et al. (2007)}
134 a new strategy?!? cyanamide 8 + cyanoacetylene 7 + glycoaldehyde 10 + glyceraldehyde 9 + inorganic phosphate*** arabanose amino-oxazoline 12 β-d-ribocytidine 2,3 phosphate (oh yeah!) Powner, Gerland, and Sutherland (2009) Nature 459,
135 the prebiotic synthesis of activated pyrimidine nucleotides should be viewed as predisposed Powner et al. (2009) Nature 459,
136 a three-fer! Although inorganic phosphate is only incorporated into the nucleotides at a late stage of the sequence, its presence from the start is essential as it controls three reactions in the earlier stages by acting as a general acid/base catalyst, a nucleophilic catalyst, a ph buffer and a chemical buffer. 1M phosphate buffer, ph 7, 40 C, o/n movie Powner, Gerland, and Sutherland (2009) Nature 459,
137 The Seven Challenges to a Prebiotic Chemist 1. The origin/source of the elements 2. The origin/source of small molecule precursors 3. The origin/source of monomers 4. The condensation problem 5. The self-replication problem 6. The chirality problem 7. The compartmentalization problem
138 Condensation polymerizing monomers with the liberation of water... in water! O H 2 N CH C OH + O H 2 N CH C OH O H 2 N CH C O H N CH C OH CH 3 CH 2 CH 3 CH 2 Ala OH Ser OH + H2O
139 activating groups and/or condensing agents were probably important for prebiotic chemistry cyanamide imidizole thioesters phosphoanhydrides (used in biology today!)
140 possible mechanisms of amino-acid condensation heating of dry amino acids to get proteinoids (Fox) thermal condensation on clay (Chang, Ferris) cyanamide-mediated synthesis (Oro)
141 Sydney Fox s proteinoids (debunked) Nature 129: (1959)
142 Thermal condensation on clay Science 201: (1978) Lahav, N., White, D., Chang, S.
143 Cyanamide-mediated polymerization (draw mechanism on whiteboard) J.Mol. Evol. 17: (1981)
144 The RNA World......needs ribose, nucleobases, and phosphates... and chains!
145 5 -GUGCCUUGCGCCGGGAAACCAC...-3 RNA structure Azoarcus ribozyme (205 nt) Adams et al. (2004) Nature 430,
146 The Catalytic Repertoire of RNA Chen, Li, & Ellington (2007)
147 The Source of Polymers NH 2 N N O N N - O P O - O H H OH O H H OH '5 A A A 3' activation is needed: triphosphate, imidizole, etc. linakage geometry is important templating can help
148 contemporary polymerases in-line nucleophilic attack Figure Schematic diagram for the nucleotidyl transferase mechanism of DNA polymerases.
149 abiotic RNA polymerization 1. high-energy condensing agents 1.1. amino acid adenylates 1.2. imidizolides 1.3. water-soluble carbodiimides 1.4. purines and pyrimidines 2. catalytic action 2.1. inorganic ions 2.2. clays 2.3. oligonucleotide templates 2.4. ribozymes 2.5. lipids
150 amino acid adenylates NH 2 N N NH 2 O N N O O P O - O H H OH O H H OH nucleotides have been proposed to condense amino acids, so can the reverse be true: AA used to condense nt s?
151 imidazolides HO: N NH 2 N O N N N R N P O - O H H OH O H H OH ImpA R = H or CH3 see P&G, Fig far more active as condensing agents, because the imidizole moiety is a good leaving group that allows for a successful attack of hydroxyl groups on a phosphorus center
152 water-soluble carbodiimides R1 N=C=N R2 example: EDC = 1-ethyl-3- (3-dimethylaminopropyl)- carbodiimide phosphoramidite
153 purines and pyrimidines NH 2 O N N P O - N N O O H H H H OH OH 4-dimethylaminopyridinium-AMP N N N O N N P H 2 N N N H 3 C O - N O O H H H H OH OH NH 2 N N adenosine-5 -phophoro-1-methyladeninium purine- and pyrimidine-like molecules are attached to the 5 phosphate and serve as good leaving groups
154 catalysts for RNA condensation: points to consider 1. template-directed vs. non-template directed 2. all 3-5 linkages vs. mixture of 3-5 and autocatalytic vs. non-autocatalytic
155 catalysts for RNA condensation ions: inorganic cations such as Zn(II), Pb(II), and UO2(II) have been demonstrated empirically to speed up RNA polymerization in the lab clays: montmorillonite clays have been demonstrated empirically to speed up RNA polymerization in the lab templates: pre-existing polymer templates have been demonstrated empirically to speed up RNA polymerization in the lab
156 example study #1: Lohrmann, Bridson, & Orgel (1980) Science 208: HPLC elution profiles of products from the template-directed selfcondensation of ImpG in the presence of (a) 0.01 M Pb(II) or (b) 0.04 M Zn(II) M ImpG, 0.04 M poly(c), 0.4 M NaNO3, 0.5 M Mg(NO3)2, 12 days, 0 C, ph 7
157 example study #2: Sievers & von Kiedrowski (1994) Nature 369: cross-catalytic schemes: auto-catalytic schemes:
158 example study #2: Sievers & von Kiedrowski (1994) Nature 369: A = CCG B = CGG Self-complementary autocatalysis has been previously demonstrated, but nucleic acid replication utilizes complementary strands, which can replicate via cross-catalysis
159 example study #2: Sievers & von Kiedrowski (1994) Nature 369:
160 example study #2: Sievers & von Kiedrowski (1994) Nature 369: AB BA, AA, and BB the addition of a particular product enhanced the rate of synthesis of that one product only
161 example study #3: Ferris et al. (1996) Nature 381: Clays to the Rescue? some aluminosilicate sheets have positive charges AND a correct spacing to fit activated nucleotides into pockets daily feeding of montmorillonite clay & a primer with activated nucleotides leads to polymerization without a template!
162 Ferris et al. (1996) Nature 381: Jim Ferris: daily feeding of nucleotides to clay results in RNA chains! longer RNA chains shorter RNA chains
163 the correct linkage and stereochemistry can be achieved Joshi, Aldersley, Zagorevskii, & Ferris (2012) Nucleosides, Nucleotides, & Nucleic Acids, in press
164 Clays: layers of ions example: Montmorillonite Jim Ferris: A key to our eventual success was the discovery that montmorillonite-catalyzed reactions of nucleotides work best when we convert clays to forms with a single kind of interlayer cation a procedure that avoids reactions or inhibition due to the metal ions bound in the interlayers of the naturally occurring montmorillonite (Banin 1973). We accomplished this conversion either by treatment of the montmorillonite with excess salts of the cation (saturation procedure) or by conversion to the acid form by acid treatment and then back titration of the hydrogen form of the clay with the desired cation. We observed that when the alkali and alkaline earth metal ions (with the exception of Mg) are the exchangeable cations, catalytically active clays are obtained.
165 The Seven Challenges to a Prebiotic Chemist 1. The origin/source of the elements 2. The origin/source of small molecule precursors 3. The origin/source of monomers 4. The condensation problem 5. The self-replication problem 6. The chirality problem 7. The compartmentalization problem
166 RNA making RNA: self-replication + + how do you transfer information from one molecule to another? balance between fidelity (for information maintenance) and errors (for evolution)
167 naturally existing catalytic RNAs group I introns (nucleotidyl transfer / transesterification) group II introns (nucleotidyl transfer / transesterification) RNase P (phosphodiester hydrolysis) ribosome (peptidyl transfer) hammerhead ribozymes (transesterification) hairpin ribozymes (transesterification) HDV ribozymes (transesterification) neurospora VS (transesterification) riboswitch ribozyme (transesterification)
168 RNA-directed catalysis in natural ribozymes phosphoester bond cleavage (hydrolysis) trans-esterification 2 -OH attack trans-esterification 3 -OH attack
169 self-cleaving ribozymes & reversibility this molecule should look familiar!
170 group I intron ribozyme Azoarcus ribozyme (205 nt) Adams et al. (2004) Nature 430,
171 in vitro selection (test-tube evolution) phenotype assay selection scheme Joyce (2007) ACIE
172 The Catalytic Repertoire of RNA Chen, Li, & Ellington (2007)
173 RNA making RNA: self-replication the holy grail of prebiotic chemistry: discovery of an RNA autoreplicase a significant advance towards this goal: the Bartel ligase ribozyme Johnston et al. (2001) Science 292, Zaher & Unrau (2007) RNA 13, Wochner et al. (2011) Science 332,
174 RNA making RNA: the Bartel/Unrau replicase ribozyme a 190-nt ribozyme that can polymerize up to 95 nt
175 polymerase chemistry: class I ligase ribozyme NNNN OH + pppn : b201 ligase (Bartel & Szostak, 1993)
176 In vitro selection of the original replicase ribozyme (2001) class I ligase ribozyme primer (orange) + template (red) replicase-14 Johnston et al. (2001) Science 292,
177 template extension by replicase-14 Johnston et al. (2001) Science 292,
178 fidelity of replicase-14 Johnston et al. (2001) Science 292,
179 In vitro selection of an improved replicase ribozyme (2007) replicase-14 in vitro selection Zaher & Unrau (2007) RNA 13, water-in-oil emulsions
180 In vitro selection of an improved replicase ribozyme (2007) replicase-20 up to 20 nt, with 3 4- fold more accuracy Zaher & Unrau (2007) RNA 13,
181 In vitro selection of an even more improved replicase ribozyme (2011) the tc19z ribozyme (replicase-95) can polymerize up to 95 nt! 95/187 = 50% Wochner et al. (2011) Science 332, replicase-95 up to 95 nt, but only certain templates
182 Eigen s error threshold Q: how accurate must a replicase be to maintain information in a population of (RNAs)? A: the length is limited by, ν < ln σ m / ln q where we are considering a selfreplicating RNA formed by ν condensation reactions, each having a mean fidelity q, where σm is the relative selective superiority of the advantageous individual compared to the remainder of the population
183 Eigen s error threshold Roughly, to maintain information, the length of a self-replicating RNA must be less than the inverse of its error rate replicase-14: fidelity = 0.967, thus μ = = νmax = 1/0.033 = 30 nt replicase-20 μ = νmax = 1/0.011 = 92 nt
184 The Origin of Chirality asymmetry is a hallmark of life modern biology: beta-d-ribonucleotides & L-amino acids it s not clear how these were selected out of a racemic mixture; moreover there is enantiomeric cross-inhibition
185 life is chiral; this is a biosignature Earth life: L-amino acids and D-nucleotides Text abiotic material is achiral or racemic
186 the origin of chirality asymmetry is a hallmark of life modern biology: beta-d-ribonucleotides & L-amino acids it s not clear how these were selected out of a racemic mixture, but possible solutions include: assistance from a chiral surface (e.g., quartz), differential precipitation or solvation, slightly different energies of the two enantiomers chiral symmetry breaking by CPL
187 enantiomeric cross inhibition could have lead to the origin of chiral synthesis? Zubay Fig ; Joyce et al. (1987)
188 The Seven Challenges to a Prebiotic Chemist 1. The origin/source of the elements 2. The origin/source of small molecule precursors 3. The origin/source of monomers 4. The condensation problem 5. The self-replication problem 6. The chirality problem metabolism FIRST? 7. The compartmentalization problem
189 Metabolism-first Theories the notion that without energy-generating mechanisms in place, life could not have originated Christian De Duve s Thioester World Gunter Wächtershäuser s Pyrite World George Cody s Nickel-iron-sulfur CO-transfer World
190 the thioester world De Duve has proposed thioesters as a key molecule to allow the build-up of larger molecules O R 1 S C R 2 De Duve: without additional help of both catalytic and energetic nature, the prebiotic broth would have remained sterile
191 origin of thioesters R 1 SH R 2 C OH thiol + O carboxylic acid energy H + O R 1 S C R 2 thioester e.g., H2S would have been abundant on the prebiotic Earth, and simple carboxylic acids could have derived from Miller- Urey type reactions
192 origin of thioesters in a hot acidic environment
193 the thiol group in thioesters is quite transferable O R 1 S C R 2 thioester + O 2H + + 2e R 1 SH + R 2 C H reducing power thiol aldehyde thioester-dependent reductions
194 the thiol group in thioesters is quite transferable O R 1 S C R 2 thioester + O HO P O OH inorganic phosphate R 1 SH thiol + O O C O P O OH acyl phosphate R 2 thioester-dependent phosphorylations
195 the thiol group in thioesters is quite transferable O R' S C R 1 + O R' S C R 2 thioester carriers O R' S C R 2 dimer + R 1 R' SH thiol thioester-dependent catalytic production of multimers
196 De Duve: thioesters were used for general activation and sequential group transfer from Blueprint for a Cell (1991)
197 the pyrite world hydrogen sulfide, in combination with the two redox states of iron, could have provided the functional precursors of all extant biochemicals FeS + H2S iron sulfide hydrogen sulfide 2H + + 2e + FeS2 reducing power pyrite Wächtershäuser views metabolism as primitive, and inventing a genetic structure later to maintain itself
198 the pyrite world at deep-sea hydrothermal vents are large columns of percipitated salts, commonly including pyrite (FeS2)
199 Wächtershäuser s chemoautotrophic origins of life local chemoautotrophic origin of life in hot volcanic exhalations by synthetic autocatalytic domino reactions of low molecular organic constituents on mineral surfaces of transition metal sulfides,
200 pyrite-pulled metabolism FeS + H2S CO2 + H2 FeS + CO2 + H2S H2 + FeS2 HCOOH HCOOH + FeS2 coupling an unfavorable reaction (the reduction of CO2) with a favorable one (pyrite production from pyrrhotite) could have led to the prebiotic fixation of carbon
201 carbon monoxide can be converted to acetic acid first, iron sulfide is carbonylated: 2FeS + 6CO + 2R-SH 2S 0 + H2 + Fe2(RS)2(CO)6 then the carbonylated Fe-S intermediate can be desulfurized to generate acetic acid and pyruvate: Fe2(RS)2(CO)6 CH3COOH + CH3-CO-COOH
202 amino acids can polymerize upon activation by CO on FeS/NiS solid surfaces Huber & Wachtershauser (1998) Science 281:
203 pyrite-pulled metabolism (draw scheme on whiteboard) FeS/H2S might be able to reduce the relatively oxidized (electron-poor) hydrocarbons such as acetylene that are present in the interstellar dust
204 the TCA cycle: at the root of anabolism generates reducing power all extant organisms oxidize chemical fuels to generate reducing power for metabolism the cycle traces both the number of carbons and their relative oxidation states
205 the reductive TCA cycle: in biology, this is catalyzed by the acetyl-coa synthase enzyme complex... using an Fe-S cluser reducing power used to fix inorganic carbon carbon fixation... performed by protein enzymes containing Fe-S clusters! the acetyl CoA pathway portion = the direct formation of acetate from CO2 or CO
206 the origins of the acetyl-coa cycle: Cody s suggestion an attractive feature of the pyrite world is the notion of life developing on a mineral surface (2D), aided by catalysts such as FeS2 also, FeS2 is similar to iron-sulfur clusters in the core of key enzymes in the TCA cycle!
207 the origins of the acetyl-coa cycle: Cody s suggestion the reactions taking place within the acetyl- CoA synthase enzyme require an Fe-S cluster at the core
208 protometabolic carbon fixation Fe-S clusters can reduce CO to a transferable methyl group
209 The Seven Challenges to a Prebiotic Chemist 1. The origin/source of the elements 2. The origin/source of small molecule precursors 3. The origin/source of monomers 4. The condensation problem 5. The self-replication problem 6. The chirality problem 7. The compartmentalization problem
210 the three stages in the evolution of life 1. chemical evolution 2. self-organization 3. biological evolution
211 the origin of cells linking genotype with phenotype compartmentalization would offer life enormous advantages keeping water concentrations low keeping local concentrations of solutes high dividing protocell into distinct compartments creating gradients allowing genotypes to harvest the fruits of their labor
212 protocell theories Oparin s coacervates Fox s proteinoid microspheres liposomes (Deamer, Szostak, etc.)
213 Oparin s coacervates μm in diameter Coacervates, which are polymer-rich collodial droplets, were studied in the Moscow laboratory of Oparin because of their conjectural resemblance to prebiological entities. These coacervates are droplets formed in an aqueous solution of protamine and polyadenylic acid. Oparin found that droplets survive longer if they can carry out polymerization reactions inside.
214 Oparin s coacervates (artificial!) Coacervates can be made by mixing: 1. proteins and carbohydrates (e.g., histones + gum arabic) 2. proteins and other proteins (e.g., histones + albumin) 3. proteins and nucleic acids (e.g., histones + RNA or DNA) Coacervates can encapsulate enzymes which are functional: phosphorylase
215 Sydney Fox s proteinoids (debunked) Nature 129: (1959)
216 liposomes when phospholipids are dissolved in water and then sonicated, the molecules tend to arrange themselves to form liposomes: closed, self-sealing, solvent-filled vesicles that are bounded by only a single layer
217 liposomes lipids can self-organize to produce small droplets (micelles) or more complex structures containing bilayers
218 liposomes monolayers can be converted to bilayers by agitation
219 phospholipids lipids are a condensation of one or more fatty acids onto a poly-alcohol (a polyol) glycerol is a tri-ol that commonly serves as a foundation for the addition of hydrophic head groups such as phosphate and hydrophobic tail groups such as fatty acids
220 phospholipids modern example
221 fatty acids long aliphatic hydrocarbon chains, with or without unsaturated C C bonds
222 amphipathic molecules self-assemble
223 lipid synthesis today 1. make fatty acid side chains 2. esterify side chains to polyol
224 lipid synthesis abiotic 1. make side chains 2. esterify side chains to polyol Fischer/Tropsch reaction C + H2O Fe, Ni Δ CnH2n+2 addition of successive CO units
225 lipid synthesis abiotic 1. make side chains 2. esterify side chains Wachtershauser s proposal CH2O FeS2 / H2S Δ (100 C, ph7) CH2 = CH2
226 lipid synthesis abiotic 1. make side chains 2. esterify side chains to polyol Art Weber s hypothesis uses glycoaldehyde as an acyl carrier is a cycle of condensation, dehydration, and isomerizations does not require ATP input can be catalyzed by metal ions
227 abiotic lipid synthesis tied to abiotic ribose synthesis through glyceraldehyde?
228 lipid synthesis abiotic 1. make side chains 2. esterify side chains to polyol glycerol + FA + phosphate, then......dehydration & rehydration
229 Artificial Cell Research Dave Deamer & Jack Szostak synthetic cells can encapsulate active enzymes: Chakrabarti et al. (1994). J. Mol. Evol. 39: synthetic cell membranes can select for ribose: Sacerdote and Szostak (2005). Proc. Natl. Acad. Sci. USA102:
230 Dave Deamer: liposome research
231 Dave Deamer: liposome research the chemiosmotic potential of membranes could have driven abiotic syntheses
232 encapsulation of polynucleotide phosphorylase (PNP) Chakrabarti AC, Breaker RB, Joyce GF, and Deamer DW (1994). Production of RNA by a polymerase protein encapsulated within phospholipid vesicles. J. Mol. Evol. 39:
233 Dave Deamer: liposome research phosporylase Chakrabarti et al. (1994). J. Mol. Evol. 39:
234 methods 1.the lipid DMPC (dimyrisoyl phosphatidyl choline) was sonicated in water 2.dry PNPase added & mixture dried under N2 gas 3.rehydration in buffer 4.extrusion through polycarbonate filters produced single-layer vesicles with encapsulated PNPase (67% ended up inside) 5. ADP added to buffer, with or without protease 6.let react several days at RT 7.radiolabel RNA and PAGE Chakrabarti AC, Breaker RB, Joyce GF, and Deamer DW (1994). J. Mol. Evol. 39:
235 results encapsulation leads to RNA polymerization! AMP not ADP used empty vesicles Chakrabarti AC, Breaker RB, Joyce GF, and Deamer DW (1994). J. Mol. Evol. 39:
236 organic material, including amphiphiles, have been found in carbonaceaous chondrites OH O monocarboxylic acids up to C10 polyaromatic hydrocarbons (PAHs): naphthalene phenanthracene anthracene
237 Dave Deamer: liposome research phospholipids extracted from meteorites can form vesicles
238 rehydration of organic extracts from meteorites can produce small vesicles Deamer (1997). Microb. Mol. Biol. Rev. 61:
239 Jack Szostak: protocell research artificial cells can be made from a variety of materials
240 methods 1.made six types of vesicles, varying the fatty acids and hence the phospholipids 2.incorporated dye into the vesicles at the same time: 5-carboxyfluorascein or calcein 3.checked for size & leakage using spectrofluorimetry and dynamic light scattering 4.put vesicles into various sugar solutions 5.conducted shrink-swell experiments using stopped-flow spectrofluorimetry 6.calculated the permeability coefficient for each sugar Sacerdote & Szostak (2005). Proc. Natl. Acad. Sci. USA 102:
241 results shrink-swell experiments: Sacerdote & Szostak (2005). Proc. Natl. Acad. Sci. USA 102:
242 conclusions: why is ribose superior? 1. ribose prefers furanose form (furanose more hydrophobic than pyranoses) 2. furanoses much more flexible than pyranoses 3. α-pyranose form of ribose has hydrophobic face (also compare Ps of erythrose and threose) Sacerdote & Szostak (2005). Proc. Natl. Acad. Sci. USA 102:
243 Jack Szostak (Harvard): making artificial cells with life-like properties Movie compartmentalization
244 in vitro evolution
245 in vitro selection (test-tube evolution) Evolution Amplification Mutation Selection selection scheme phenotype assay Joyce (2007) ACIE
246 in vitro evolution
247 (Systematic Evolution of Ligands by Exponential Enrichment)
248 rough numbers what can be selected: RNA, DNA, proteins original pool (G0) size: molecules mutation methods: error-prone PCR mutator oligos errors in non-amplifying replication environmental stress (UV, mutagens, etc.) selection strategies binding tagging size other sequence attributes number of generations needed to get a winner : about 6
249 creating G0
250 selecting winner(s)
251 amplifying winner(s) the polymerase chain reaction (PCR)! if you are working with DNA, PCR directly if you are working with RNA, turn RNA into DNA first using reverse transcriptase (RT) if you are working with proteins, PCR the gene for the protein (or make virus do it: phage display)
252 the polymerase chain reaction (PCR) extract genomic DNA design primers do PCR reaction amplification!
253 the polymerase chain reaction (PCR) 1967: Gobind Khorana, comes up with the idea of replicating DNA in vitro 1983: Kery Mullis, working at Cetus, develops the idea of using Taq DNA polymerase and thermal cycling 1985: Randall Saiki et al. publishes the first actual report of PCR in Science 1993: Mullis wins the Nobel Prize in Chemistry for PCR
254 the polymerase chain reaction (PCR)
255 but let s go back to the 60 s
256 bacteriophage Qβ replicase gene: codes for an RNA-dependent RNA replicase protein that copies the 3300 nt phage genome
257 Sol Spiegelman (1967) Proc. Natl. Acad. Sci USA (1967) 58,
258 Sol Spiegelman (1967) in vitro ( extracellular ) serial transfer experiments Qβ RNA Qβ replicase nucleotides buffer 20 minutes 20 minutes 20 minutes 20 minutes etc. original wild-type Qβ stock assay RNA for genotype and phenotype
259 result #1 continuous growth of RNA etc.
260 result #2 infectivity drops over time etc.
261 result #3 some sort of sequence evolution is happening etc.
262 result #4 selection for much shorter RNAs! original sequence: 3300 nt etc. evolved sequence: 550 nt
263 later experiments: resistance to ethidium bromide or RNase etc.
264 1980 s: along comes the PCR selection for aptamers (SELEX) selection of a ribozyme that can cleave DNA as well as RNA (selection of a ligase ribozyme) evolution of a ligase ribozyme (selection of a polymerase ribozyme) etc. etc.
265 selection of a DNAcleaving ribozyme selection strategy Beaudry & Joyce (1992) Science 257:
266 selection of a DNAcleaving ribozyme mutations of wildtype = G0 the Tetrahymena group I intron (self-splices in vitro) Beaudry & Joyce (1992) Science 257:
267 selection of a DNAcleaving ribozyme G0 G3 G6 G9 phenotype genotype Beaudry & Joyce (1992) Science 257:
268 selection of the class I ligase ribozyme 14 rounds of in vitro selection b201 ligase (Bartel & Szostak, 1993)
269 class I ligase ribozyme continuous evolution of the ligase ribozyme
270 class I ligase ribozyme continuous evolution of the ligase ribozyme
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