DEVELOPMENT OF PFCs HIGH SENSITIVITY ANALYSIS METHOD APPLIED RETENTION GAP TECHNIQUE WITH UPLC/MS/MS Tatsuya Ezaki 1, Nobutake Sato 1, Jun Yonekubo 1,

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1 DEVELOPMENT O PCs HIGH SENSITIVITY ANALYSIS METHOD APPLIED RETENTION GAP TECHNIQUE WITH UPLC/MS/MS Tatsuya Ezaki 1, Nobutake Sato 1, Jun Yonekubo 1, Takeshi Nakano 2 1 Nihon Waters K.K, Kitashinagawa, Shinagawa-ku Tokyo-to Japan. tatsuya_ezaki@waters.com 2 Hyogo Prefectural Institute of Environmental Sciences, , Yukihira-cho, Suma-ku, Kobe-city Hyogo-ken, Japan. Abstract Liquid chromatography/tandem MS/MS system is used to analyze Per fluorinated compounds (PCs) without derivatization, quantifying trace levels of PCs in samples unambiguously is challenging because of the widespread back ground PCs contamination. Since PCs are present in many conpornents of lab instruments, trace levels of PCs can leach out. In addition, PCs are also detected in common HPLC solvents and in lab water. Because background PCs contamination is pervasive, quantifying trace levels of PCs requires special care. With online removal of PCs in mobile phase by solid phase extract ion (SPE) cartridge or technique applied retention gap, the contaminated PCs can been reduced or shifted off. Introduction Per fluorinated compounds (PCs) such as Perfluorooctanoic acid (POA) and Perfluorooctane sulfonate (POS) have been used for over 50 years in various applications that include surfactants, element in materials of chrome plating, fire fighting foam, photoresist on semiconductor manufacture and polymerization aid in synthesis polytetrafluoroethlene (PTE), and fluorpolymers 1. PCs are extremely stable and not prone to environmental degradation. Long-chain PCs such as POA and POS bioaccumulate in animals causing tumors and disturbing reproductive development 2. Trace levels of OH O PCs have been measured in groundwater, wastewater treatment plants, lake water, m O n O S OH the marine environment and even in the Arctic. In recent reports studies, POA, POS and other PCs have been detected at parts per billion levels in wildlife tissues and human serum. igure 1. Chemical structures of PCs : m=2, PBA; m=3, PPeA; m=4, PHxA; m=5,phpa; m=6, POA; m=7, PNA; m=8, PDA; m=9, PUdA; m=10, PDoA; m=11, PTrA; n=3, PBS; n=5, PHxS; n=7, POS; n=9, PDS. Compounds page

2 Literature reports on the potential impact of PCs on human health and the environment indicate that this is a global concern. Consequently, there is an increased demand for rapid, sensitive and accurate analytical methods for the analysis of PCs in environmental and biological matrics. Materials and Methods SPE: OASIS HLB and SepPak AC2 used as SPE cartridges for on-line cleanup were obtained from Waters. The packing particle of OASIS HLB is reverse phase based hydrophilic-lipophilic balanced co-polymer structure and traps PCs of long chain length. SepPak AC2 is packed the particle of activated carbon and irreversibly traps PCs of short chain length. PCs Standard Solution: PC-MXA mixed Perfluorocarboxylic acid (PCAs), PS-MXA mixed Perfluoroalkyl sulfonate (PASs) and MPAC-MXA mixed surrogate ( 13 C labeled) of PCAs were all obtained from Wellington Laboratory Inc and were used as standard solution. All standard solutions were diluted with Methanol that was HPLC grade (Kanto Chemical Co.,Inc.).. Ammonium acetate and Acetonitrile (HPLC grade) were purchased from Kanto Chemical Co.,Inc. and used as mobile phase. LC MS/MS analysis: The LC apparatus was an Acquity Ultra Performance LC(Waters). All analytes were separated using a Waters Acquity UPLC BEH C18 column (50mm 2.1mm, 1.7um particle size) and Acquity UPLC BEH Shield RP18 column (100mm 2.1mm, 1.7um particle size). On the case of on-line cleanup of PCs in mobile phase and method applied retention gap technique, analytical condition of UPLC/MS/MS is shown in Table 1. Mass spectrometry was performed using a Waters TQ detector operated with an ESI interface in negative ionization mode. The capillary voltage was set at kv. The flow rates of desolvation gas and cone gas were set to 1000 and L/h, respectively. The source temperature and desolvation gas temperature were held at 120 and 400 C, respectively. Quantitative analysis was performed in multiselected reaction monitoring (MRM), and the one or two most abundant MRM transitions were listed in Table 2. Results and Discussion Method with on-line cleanup Three SPE cartridges (reverse-phase, activated carbon and reverse-phase) were installed on the line of Mobile Phase A (ig.2). PBA, PPeA and PHxA in mobile phase A were irreversibly trapped on these cartridges and baseline can been shown low and stable noise level, additionally, the peaks of PCs contaminated from LC system and mobile phase A can been disappeared (ig 3). When SPE cartridge columns capacity were overloaded with contaminated PCs, their peaks were appeared (ig.4). Compounds page

3 Table 1 Analytical conditions of UPLC/MS/MS Table 1-1. UPLC/MS/MS method with on-line cleanup UPLC Conditions Column: UPLC BEH Shield RP18 2.1mmx100mm 1.7um Mobile Phase A: water containing 2mM Ammonium Acetate Mobile Phase B: Acetonitrile/ water in 2mM Ammonium Acetate (95/5) Gradient table and low Rate low rate (ml/min) (min) Conc. of A Conc. of B Gradient curve 0.4 Initail Table 1-2. UPLC/MS/MS method with retention gap UPLC Conditions Column: ACQUITY UPLC BEH C18 2.1mmx50mm 1.7um Retention gap: ACQUITY UPLC BEH C18 2.1mmx100mm 1.7um Mobile Phase A: water containing 10mM Ammonium Acetate Mobile Phase B: Acetonitrile Gradient table and low Rate low rate (ml/min) (min) Conc. of A Conc. of B Gradient curve 0.3 Initail Mobile Phase A Reverse Phase: OASIS HLB Trap of long chain length PCs Activated Carbon: SepPak AC2 Trap of short chain length PCs Reverse Phase: OASIS HLB ilter for activate carbon UPLC Pump / mixer Injector / Analytical column ig.2 : Three SPE cartridges were installed on the line of Mobile Phase A Compounds page

4 Table 2 Multi-selected reaction monitoring (MRM) transitions of PCs Table 2-1. MRM transitions of PCAs Table 2-2. MRM transitions of PASs PC MRM CV (V) CE PC MRM CV (V) CE PBA > MPBA > PPeA > PBS > > PHxS > PHxA MPHxA PHpA POA MPOA PNA MPNA PDA MPDA PUdA MPUdA PDoA MPDoA PTrDA PTeDA > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > > MPHxS > POS > > MPOS > > PDS > > Compounds page

5 ig.5 : Method applied retention gap technique PCs contaminated in mobile phase A are trapped with retention gap column installed before injector. Each contaminated PCs elute on delay time for PCs in sample Compounds page

6 _05 1: MRM of 6 Channels ES > _01 1: MRM of 6 Channels ES > _05 1: MRM of 6 Channels ES > _05 1: MRM of 6 Channels ES > _01 1: MRM of 6 Channels ES > e _01 1: MRM of 6 Channels ES > e ig.3: MRM chromatograms when Methanol were injected with SPE cartridges on the line of mobile phase A ig.4: MRM chromatograms when SPE overloaded with contaminated PCs Method applied retention gap technique PCs contaminated in mobile phase A are trapped with retention gap column installed before injector. At the same time of sample injection, gradient program is started. Accuracy on quantitative analysis of PCs can be improved with the time difference occurred between retention time of PCs in sample Test034 1: MRM of 6 Channels ES > (PBA01) e ig. 6 MRM chromatograms of PBA with method applied retention gap technique and contaminated PCs because contaminated PCs are trapped on retention gap column (ig.5). ig. 6 shows MRM chromatograms of PBA and PBA in injected sample is detected as the peak that retention time is 2.81 minutes and the broad peak from 3.24 minutes is PBA contaminated from mobile phase A. Conclusion UPLC/MS/MS method with higher sensitivity and separation efficiency was established for analyzing 11 PCAs and 4 PASs and was higher accuracy on quantitative analysis because PCs in samples can be separated PCs contaminated from mobile phase with retention gap column. The method applied retention gap technique has issue that the pressure is higher on LC pump because retention gap and analytical column are installed, but has the stability on continuous injection and higher reliability when compare with the method of on-line clean up mobile phase. References 1. J W Washington, et al., J. Chromatogr. A, 1154, , J M laherty, et al., J. Chromatogr. B, 819, , Compounds page