Bulletin 858C. Petroleum/Chemicals Application Guide. Detailed Hydrocarbons Analyses

Transcription

1 Bulletin C Petroleum/Chemicals Application Guide This guide contains up-to-date information about Supelco products and technology for separating hydrocarbons by chromatographic methods. Key Words: petroleum chemicals hydrocarbons Applications Hydrocarbons Analyses -0 Oxygenates 0 Simulated Distillation - Aliphatics / Aromatics - Polynuclear Aromatic Hydrocarbons Trace Petroleum Components Miscellaneous Petrochemicals Analyses - Phenols / Polymers Solvents 0- Impurities in Solvents - High Speed / High Resolution Separations - Packed Column Applications - Gel Permeation of Polymers Low Molecular Weight Compounds - Detailed Hydrocarbons Analyses Nonpolar 0-Meter Capillary Column for High Resolution of Hydrocarbons (ASTM D) Information about the paraffin, isoparaffin, olefin, aromatic, and naphthene (PIANO) content of many products is critical for process control and product quality testing. We developed the 0m x 0.0mm ID Petrocol DH 0. column specifically for this type of application (Figures -). This high efficiency, bonded, nonpolar (methyl silicone) phase column typically offers 000 plates/meter. Figure. Butane Lighter Fuel. nc. ic. nc Simple Hydrocarbon Mixtures Column: Petrocol DH 0., 0m x 0.0mm ID, 0.µm film : -U Oven: C (0 min) to 00 C at C/min Carrier: helium, 0cm/sec (set at C), C Inj.: C Petroleum Ether. ic. nc.,-dimethylbenzene. Cyclopentane. -Methylpentane. -Methylpentane.,-Dimethylbutane Ordering Information Columns and Packings - Laboratory Accessories Chemical Standards and Accessories Figure. Unleaded Gasoline 0. nc. -Methylpentane. nc. Benzene. nc. Toluene. nc. Ethylbenzene. m-xylene 0. p-xylene. o-xylene. nc Column and conditions: see Figure A. -00 Bulletin T00C Sigma-Aldrich Co.

2 Figure. Detailed Analysis of Qualitative Reference & Naphtha Column and conditions: see Figure. -0 Figure. Quantitative PIANO Standard. nc. nc. Benzene. nc. Toluene. nc. m-xylene. nc. ic. nc. ic. nc. -Methylpentane. nc. Methylcyclopentane. Benzene 0. Cyclohexane. p-xylene. o-xylene 0. nc. nc0. nc. nc. nc 0. nc. Methylcyclohexane. Toluene. nc. m-xylene. p-xylene. nc. nc0. nc 0. nc 0 Gasoline Range Petroleum Products Petrocol DH columns (Figures -) can separate more than 00 gasoline components in less than 0 minutes under ambient initial temperature conditions. They are ideal for PONA, PNA, and PIANO analyses. Nonpolar Petrocol DH columns provide greater separation of propane and C compounds, m- and p-xylene (Figure ), and other light petroleum components. Because you can use Petrocol DH columns with modest split ratios (approximately 00:), you can simultaneously monitor trace and concentrated sample components. Our library of retention indices for more than 00 compounds is included with column purchase. Figure.,-Dimethylbutane from Cyclopentane Column: Petrocol DH, 00m x 0.mm ID, 0.0µm film : 0-U Oven: C or C ( min) to 00 C at C/min, hold min Carrier: helium, 0cm/sec Inj.: synthetic blend (% cyclopentane, %,-dimethylbutane), split (00:). Cyclopentane.,-Dimethylbutane C C Column and conditions: see Figure % Return to Baseline Complete Separation Figure. Quantitative PIANO Standard, Using Rapid Temperature Program 0. nc. nc. Benzene. nc. Toluene. nc. m-xylene. p-xylene. o-xylene 0. nc. nc0. nc. nc. nc PONA: paraffins-olefins-naphthenes-aromatics PNA: paraffins-naphthenes-aromatics PIANO: paraffins-isoparaffins-aromatics-naphthenes-olefins -, Column and conditions: same as Figure, except Oven: C ( min) to 00 C at C/min. -00 Bulletin

3 Figure. Individual Components of Premium Unleaded Gasoline Column: Petrocol DH, 00m x 0.mm ID, 0.0µm film : 0-U Oven: C ( min) to 00 C at C/min, hold min Carrier: helium, 0cm/sec Inj.: 0.µL gasoline, split (00:). Isobutane. n-butane. Isopentane. n-pentane.,-dimethylbutane. -Methylpentane. -Methylpentane. n-hexane..,-dimethylpentane 0. Benzene. -Methylhexane. -Methylhexane.,,-Trimethylpentane. n-heptane.,-dimethylhexane.,-dimethylhexane.,,-trimethylpentane. Toluene.,-Dimethylhexane 0. Ethylbenzene. m-xylene. p-xylene. o-xylene. -Methyl--ethylbenzene.,,-Trimethylbenzene.,,-Trimethylbenzene.,,-Trimethylbenzene. Naphthalene. -Methylnaphthalene 0. -Methylnaphthalene. Dimethylnaphthalenes , 0 Bulletin

4 Figure. A Difficult Separation: Isobutylene from Butene- Column: Petrocol DH, 00m x 0.mm ID, 0.0µm film : 0-U Oven: -0 C (0 min) to 00 C at C/min Carrier: helium, cm/sec Inj.: µl Phillips L blend, split (00:). Propylene. Propane. Isobutane.,-Butadiene. n-butane. trans--butene. cis--butene Isobutylene -Butene Figure. Light Hydrocarbons Using Moderately Subambient Initial Temperature Column: Petrocol DH 0, 0m x 0.mm ID,.0µm film : Oven: -0 C (0 min) to C at C/min Carrier: helium, 0cm/sec Inj.: 0µL nitrogen (0.-% each analyte), split (00:) Isobutylene. Ethane. Propylene. Propane. Isobutane. Isobutylene. -Butene. n-butane. trans--butene. cis--butene 0. Isopentane. -Pentene Isobutylene and -Butene Also Are Well Resolved at 0 C Initial Temperature -Butene , Ambient Separations of Hydrocarbon Gases Very high efficiency (typically 0,000 theoretical plates) and a low phase ratio (0.mm ID,.0µm phase film) enable our 0-meter Petrocol DH 0 column to separate light hydrocarbons significantly better than other columns. Use Petrocol DH columns to analyze hydrocarbon mixtures ranging from the light gases to the higher boiling components of gasoline and other samples of comparable boiling range (Figures -). Perform most of these analyses using ambient or mildly subambient (0 C or -0 C) initial temperatures. Perform separations that previously required special conditions, unusual columns, or prolonged analysis time. Head pressure of approximately psig will provide the optimum linear velocity, 0cm/sec, for helium carrier gas. If your instrument cannot provide this pressure, consider using hydrogen as the carrier gas (0-0psig will provide the optimum 0cm/sec linear velocity), or consult your instrument manufacturer about alternative pressure regulators. Using an ambient initial temperature, gasoline analyzed on a Petrocol DH 0 column shows near baseline resolution of the light hydrocarbons cyclopentane and,-dimethylbutane, and the heavier m-xylene and p-xylene isomers (Figure ). Under these conditions, separations of the light hydrocarbons through toluene are optimized by using the isothermal hold period. The subsequent temperature program enables analysts to obtain excellent detail for the heavier compounds. Conditions can be varied (e.g., analysis time shortened) to meet specific needs. Figure 0. Gaseous Hydrocarbons from Liquid Hydrocarbons Column: Petrocol DH 0, 0m x 0.mm ID,.0µm film : Oven: C Carrier: helium, 0cm/sec Inj.: µl natural or liquified petroleum gas, split (00:) Natural Gas Standard. Methane. Ethane. Propane. Isobutane. n-butane. Isopentane. n-pentane Liquified Petroleum Gas Standard. Ethane. Propylene. Propane. Isobutane. n-butane. Isopentane ,0 Bulletin

5 Figure. Oxygenated Compounds in a C Gas Stream, Using Ambient, Isothermal Temperature Column: Petrocol DH 0, 0m x 0.mm ID,.0µm film : Oven: C Carrier: helium, 0cm/sec Inj.: 0µL nitrogen (0.-% each analyte), split (00:). Ethane. Propylene. Propane. Dimethylether. Isobutane. Methanol. Isobutylene. -Butene. n-butane 0. trans--butene. cis--butene. Ethanol. n-pentane. Methyl tert-butyl ether Figure. Detailed Analysis of Gasoline, Using Ambient Initial Temperature Column: Petrocol DH 0, 0m x 0.mm ID,.0µm film : Oven: C ( min) to 00 C at C/min Carrier: helium, 0cm/sec Inj.: 0.µL unleaded gasoline, split (00:) 0. n-butane. Isopentane. n-pentane.,-dimethylbutane. Cyclopentane.,-Dimethylbutane. -Methylpentane. -Methylpentane. n-hexane 0.,-Dimethylpentane. Benzene. -Methylhexane.,-Dimethylpentane. -Methylhexane.,,-Trimethylpentane. n-heptane.,-dimethylhexane.,-dimethylhexane.,,-trimethylpentane 0. Toluene.,,-Trimethylpentane.,-Dimethylhexane. -Methylheptane. -Methylheptane. -Methyl--heptene. n-octane. Ethylbenzene. m-xylene. p-xylene 0. o-xylene. -Nonene. n-nonane. Isopropylbenzene.,,-Trimethylheptane.,,-Trimethylheptane. n-propylbenzene. -Methyl--ethylbenzene. -Methyl--ethylbenzene.,,-Trimethylbenzene 0.,,-Trimethylheptane. -Methyl--ethylbenzene.,,-Trimethylbenzene. Isobutylbenzene. sec-butylbenzene. n-decane.,,-trimethylbenzene. Indane.,-Diethylbenzene. n-butylbenzene 0.,-Dimethyl--ethylbenzene.,-Dimethyl--ethylbenzene.,-Dimethyl--ethylbenzene.,,,-Tetramethylbenzene.,,,-Tetramethylbenzene. Naphthalene. -Methylnaphthalene. -Methylnaphthalene. Dimethylnaphthalenes Bulletin ,

6 Reproducible Results The highly reproducible Petrocol DH Octyl capillary column is capable of resolving compounds of different classes to the baseline (Figures to ). Table A summarizes some key shifts in elution order. As a general rule, polar compounds shift to shorter retention times relative to normal hydrocarbons. Figure. Key Hydrocarbon Pairs Completely Resolved Column: Petrocol DH Octyl, 00m x 0.mm ID, 0.µm film : Oven: 0 C to 0 C at C/min Carrier: helium, cm/sec, 0 C Inj.: 0.µL, split :, 0 C Figure. Pyrolysis Gasoline (Py Gas) Column: Petrocol DH Octyl, 00m x 0.mm ID, 0.µm film : Oven: C ( min) to 00 C at C/min ( min) Carrier: helium, cm/sec, 0 C Inj.: 0.µL, split :, 0 C 0- min. -Methylpentane. -Methylpentane. n-hexane. Methylcyclopentane. Benzene. -Methylcyclopentene. Benzene. -Methylcyclopentene. Toluene.,,-Trimethylpentane min. Ethylbenzene. p-xylene. m-xylene. Styrene. o-xylene Figure. PONA V Mix Column: Petrocol DH Octyl, 00m x 0.mm ID, 0.µm film : Oven: C ( min) to 00 C at C/min ( min) Carrier: helium, cm/sec, 0 C Inj.: 0.µL, split :, 0 C. Ethane. Propane. Methanol. Isobutane. Ethanol. Isopropanol. Isopentane. tert-butanol, Pentene-. -Methylbutene- 0. n-pentane. trans-pentene-. cis-pentene-. -Methyl--butene. Methyl tert-butyl ether. -Methylpentane. -Methylpentane. n-hexane. Ethyl tert-butyl ether. Benzene 0. -Methylcyclopentene Table A. Selected Elution Shifts Between Petrocol DH Octyl and SPB- Columns -0,0 of Elution Petrocol DH Class Compounds SPB- Octyl A Benzene Coelution Baseline O -Methylcyclopentene A Toluene Coelution Baseline I,,-Trimethylpentane A p-xylene Partial Baseline A m-xylene (reversed) P n-paraffin Baseline Coelution O trans--olefin OXY Methanol Partial Baseline Bulletin

7 Difficult Petroleum Analyses Use a Petrocol DH column for analyses ranging from resolving closely eluting isomers or light hydrocarbon gases to detailed separations of highly complex mixtures such as petroleum naphthas and reformates and liquified coal fractions (Figures -0). Figure. Monitor Dicyclopentadiene for Breakdown Products Column: Petrocol DH, 00m x 0.mm ID, 0.µm film : 0-U Oven: C ( min) to 0 C at C/min ( min) Carrier: helium, 0cm/sec Figure. Closely Eluting Hexene Isomerization Products Column: Petrocol DH, 00m x 0.mm ID, 0.µm film : 0-U Oven: C ( min) to C at C/min (0 min) Inj.: µl mixed hexene isomers, split 00:. cis--methyl--pentene. trans--methyl--pentene. -Methyl--pentene. cis--methyl--pentene. trans--methyl--pentene 0 C Injection Port Temperature Separation to baseline DCPD Higher Analogs Boiling points differ by only. C Analysis complete in less than 0 minutes Essentially No CPD Monomer Impurities Impurities 0 C Injection Port Temperature 0-00 CPD Monomer (reveals sample decomposition) DCPD Figure. Light Hydrocarbon Gases Using a Subambient Initial Temperature Inj.: 0.µL DCPD, split 00: Figure. Quantify ENBV Isomers and VNB Isomers Column: Petrocol DH, 00m x 0.mm ID, 0.µm film : 0-U Oven: C ( min) to 00 C at C/min Inj.: 0.0µL ENB/VNB mixture, split 00: 0-. endo-vnb. exo-vnb. trans-enb. cis-enb Column: Petrocol DH, 00m x 0.mm, 0.µm film : 0-U Oven: min, at - C, then to 0 C at C/min and hold min Carrier: helium, cm/sec (set at C) Det.: x 0 - AFS Inj.: 00µL, split (00:). Methane. Ethylene. Acetylene. Ethane. Propylene. Propane 0. Propadiene. Isobutane. Isobutylene 0. -Butene.,-Butadiene. n-butane. trans--butene. cis--butene. Isopentane. -Pentene. n-pentane Approximately 0% return to baseline 0-00 Bulletin

8 Figure 0. Petroleum Reformate Column: Petrocol DH, 00m x 0.mm ID, 0.µm film : 0-U Oven: C ( min) to 00 C at C/min (0 min) Inj.: 0.µL reformate,. Benzene split 00:. Toluene. Ethylbenzene. m-xylene. p-xylene. o-xylene. -Methyl--ethylbenzene.,,-Trimethylbenzene. Naphthalene 0. -Methylnaphthalene. -Methylnaphthalene. Dimethylnaphthalenes Better than 0% return to baseline C-C0 aromatics well resolved Light Hydrocarbons Monitor Light Hydrocarbons Without Using Cryogenics Because they do not require cooling to retain compounds with low (-0 C to 00 C) boiling temperatures, thick film SPB - and SPB- capillary columns are more convenient to use than other types of columns. Typical analyses are shown in Figures and. When you need additional resolution, you still have the option of using subambient initial temperatures (Figure inset). SPB- and SPB- thick film columns are available in to 0 meter lengths (0.mm ID fused silica) and µm and µm film thickness. For a complete offering of these columns, see our general catalog. Figure. C-C Hydrocarbons and C-C Alcohols on Thick Film Capillary Column Column: SPB-, 0m x 0.mm ID, µm film : Oven: 0 C ( min) to 00 C at 0 C/min (inset: initial temp. 0 C) Carrier: helium, -cm/sec, 0 C Inj.: 0µL (balance N ), split 00:, 00 C Higher Resolution of Polynuclear Aromatic Hydrocarbons Naphthalene -Methylnaphthalene -Methylnaphthalene Dimethylnaphthalenes 0 0- Component Concentration (Vol. %). Methane 0.. Ethylene 0.. Ethane 0.. Propylene 0.. Propane 0.. Propadiene 0.. iso-butane 0.. Methanol 0.. Butene n-butane 0.. cis--butene 0.. Ethanol 0.. iso-propanol 0.. n-pentane 0.. tert-butanol 0.. n-propanol 0.. Methyl tert-butyl ether 0.. sec-butanol 0.. n-hexane iso-butanol 0.. n-butanol 0.. Benzene 0. iso-butane and Methanol at 0 C Initial Temperature, ,00 Bulletin

9 Figure. Natural Gas on Thick Film Column Column: SPB-, 0m x 0.mm ID, µm film : Oven: 0 C ( min) to 00 C at 0 C/min Carrier: helium, -cm/sec, 0 C Inj.: 0µL (balance N ), split 00:, 00 C Concentration (Vol. %). Methane 0.. Ethane.0. Propane.0. iso-butane.0. n-butane.0. iso-pentane.0. n-pentane.0 Helium/Nitrogen balance High Boiling Hydrocarbons Better Quantitation of High Boiling Compounds For analyses of paraffin mixtures, SPB- Thin Film bonded phase capillary columns (Figures -) offer better sample resolution and more reliable quantitation than packed columns. When you use an SPB- Thin Film column with our cool on-column injection sleeve (designed for / packed column or capillary injection ports), you can virtually eliminate discrimination among sample components. Use a syringe with a (.cm) needle to make injections. Figure. Polywax Column: SPB- Thin Film, m x 0.mm ID, 0.µm film : 0 Oven: 0 C to 0 C at C/min Carrier: helium, 0mL/min (flow controlled), 0 C Inj.: µl, cool on-column injection. nc0. nc0. nc0. nc0. nc0. nc0. nc Figure. Shoe Polish Column: SPB- Thin Film, m x 0.mm ID, 0.µm film : 0 Oven: 0 C to 0 C at C/min Carrier: helium, ml/min (flow controlled), 0 C Inj.: µl, cool on-column injection. nc. nc0. nc. nc0. nc. nc0. nc Bulletin

10 Figure. Paraffin Wax Column: SPB- Thin Film, 0m x 0.mm ID, 0.µm film : Oven: 0 C to 0 C at C/min Carrier: helium, ml/min (flow controlled), 0 C Inj.: µl, cool on-column injection. nc (int. std.). nc0. nc0. nc0 Figure. MTBE Contaminants on a 00-Meter Petrocol DH Octyl Column Column: Petrocol DH Octyl, 00m x 0.mm ID, 0.µm film : Oven: C ( min) Carrier: helium, cm/sec (set at C), 0 C Inj.: 0.µL, split :, 0 C. Methanol. Isopentane. tert-butanol. -Methyl--butene. MTBE. ETBE. TAME Oxygenates Separate Oxygenated Compounds in Complex Hydrocarbon Matrices Because of the high toxicity of organic bound lead, the US EPA has mandated the reduction and eventual elimination of lead compounds in gasoline. The compounds substituted for lead are usually oxygen-containing substances such as alcohols and ethers. Separation of these oxygenates from the matrix can be a problem. Our Petrocol DH Octyl and Petrocol DH 0 capillary columns (Figures to ) are excellent for this type of analysis. Figure. Oxygenates Mix on a 00-Meter Petrocol DH Octyl Column Column: Petrocol DH Octyl, 00m x 0.mm ID, 0.µm film : Oven: C ( min) to 00 C at C/min ( min) Carrier: helium, cm/sec, 0 C Inj.: 0.µL, split :, 0 C. Methanol. Ethanol. Isopropanol. tert-butanol. MTBE. Isobutanol. sec-butanol. ETBE. Benzene 0. TAME Figure. Oxygenates on a Petrocol DH 0 Column Column: Petrocol DH 0, 0m x 0.mm ID,.0µm film : Oven: C Carrier: helium, 0cm/sec (set at C), 00 C Inj.: approx. 0.-% each component in 0µL nitrogen, split 00:, 00 C Ethane. Propylene. Propane. Dimethyl ether. Isobutane. Methanol. Isobutylene. -Butene. n-butane 0. trans--butene. cis--butene. Ethanol. n-pentane. Methyl tert-butyl ether Bulletin

11 Simulated Distillation Figure 0. Gasoline by ASTM D0 Packed Columns for More Reliable SIMDIS Analyses Petrocol A and Petrocol B packed columns were developed specifically for Methods D0 and D, respectively, of the American Society for Testing and Materials (ASTM). These 0 x / stainless steel columns minimize peak tailing, column bleed, and the deviation of apparent boiling point from true boiling point (Figures -). Figure. Petrocol A Column imizes Hydrocarbon Peak Tailing Column: (A) Petrocol A, 0" x /" SS : Col. Temp.: -0 C to 00 C at 0 C/min Carrier: helium, ml/min ( x 0-0 AFS) Inj.: 0.µL of D0 Qualitative Mix ( -) Column: Petrocol A, 0" x /" SS : Col. Temp.: -0 C to 0 C at C/min Carrier: helium, ml/min ( x 0-0 AFS) Inj.: 0.µL of gasoline Cs Toluene Xylenes Cs Cs Petrocol A Column n-propane. Methylpropane. n-butane. -Methylbutane. n-pentane. -Methylpentane. n-hexane.,-dimethylpentane. n-heptane 0. Toluene. n-octane. p-xylene. n-propylbenzene. n-decane. n-butylbenzene. n-dodecane. n-tridecane. n-tetradecane. n-pentadecane 0 0 Figure. Hydrocarbon Boiling Point and Retention on a Petrocol A Column Are Closely Correlated 00 n-paraffins Branched Paraffins -0 0% UC-W on 0/00 Chromosorb P AW 00 0 Aromatic hydrocarbons and other sample components are tailing Boiling Point ( F) n-butylbenzene n-propylbenzene p-xylene Toluene 0 0 Analytical conditions as in Figure (Sec.) -0, - Bulletin

12 Figure. imize Hydrocarbon Peak Tailing and Column Bleed for Method D Column: (top figure) Petrocol B, 0" x /" SS : Col. Temp.: - C to 0 C at C/min Carrier: helium, 0mL/min ( x 0-0 AFS) Inj.: 0.µL of mixed aliphatics and aromatics Toluene nc nc nc Petrocol B Column: Negligible Bleed at 0 C n-butylbenzene nc0 nc nc nc nc nc n-decylbenzene nc nc0 Figure. Negligible Column Bleed for Gas Oil Capillary Columns for SIMDIS Analysis Column: Petrocol B, 0" x /" SS : Col. Temp.: - C to 0 C at C/min Carrier: helium, 0mL/min ( x 0-0 AFS) Inj.: 0.µL reference gas oil nc nc nc nc nc0 nc nc 0% UC-W on 0/00 Chromosorb P AW: High Bleed at 0 C nc0 nc nc0 nc0 nc nc nc nc0 nc nc -00, Baseline returns to near zero at 0 C Capillary Columns for SIMDIS Analysis If you use packed columns to perform simulated distillation (SIMDIS) analyses of gasoline range petroleum products, Petrocol 0 and Petrocol capillary columns can improve column performance and your results for ASTM Methods D0 and D, respectively (Figures - and Table B). These columns offer important advantages over even the most reliable packed columns: Longer retention of early-eluting hydrocarbons for a more linear boiling point/retention time curve (Figures -) Low column bleed for a sharp return to baseline and more reliable final boiling point data Symmetrical peaks Rapid conditioning ( minutes to hour) A potentially longer column lifetime, because the phase is bonded You can connect these capillary columns to packed column injection ports and detectors by using our inexpensive adapter kits. Use the columns with packed column flow controllers and most autosamplers. Petrocol capillary columns satisfy all column performance criteria in the ASTM methods. Figure. Petrocol 0 Capillary Column Provides Near-Linear Correlation Between Hydrocarbon Boiling Point and Retention Time Boiling Point, F Boiling Point, F Packed Column, -0 C Initial Temperature C C C Typical Curvature at Low End C 0 0 Petrocol 0 Capillary Column, Ambient (0 C) Initial Temperature C C C Petrocol 0 Capillary Column, -0 C Initial Temperature C C 0 0 nc Boiling Point, F C C C Less Curvature at Low End 0 0 -,, Bulletin

13 Figure. Linear Boiling Point/Elution Time Relationship for n-paraffins on a Petrocol Column 00 Figure. Column: Col. Temp.: Carrier: Det.: Inj.: SIMDIS Analysis of Gasoline Petrocol 0, 0m x 0.mm ID glass,.0µm film s -0 C to 00 C at 0 C/min nitrogen, ml/min FID 0.µL of a commercial gasoline 000 nc nc0 nc nc Detector Sensitivity 0 x 0-0 Boiling Point, F nc nc nc nc0 nc nc nc nc nc nc0 nc nc nc Sharp and Precise Return to Baseline with Negligible Bleed at 00 C Retention Time, Seconds -0 Figure. D0 Qualitative SIMDIS Standard Column: Petrocol 0, 0m x 0.mm ID glass,.0µm film s Col. Temp.: -0 C to 00 C at 0 C/min Carrier: nitrogen, ml/min Inj.: 0.µL of D0 Qualitative Mix ( ) p-xylene Toluene Figure. D SIMDIS Calibration Blend Column: Petrocol, m x 0.mm ID, 0.µm film : Col. Temp.: -0 C to 0 C at 0 C/min, hold min Carrier: nitrogen, ml/min ( x 0-0 AFS) Inj.: 0.µL Quantitative Calibration Mix ( ), direct n-butane. nc. nc. nc. nc. nc0. nc. nc. nc. nc 0. nc. nc0. nc. nc. nc. nc. nc0. nc Isobutane Propane n-c }R =. n-c } 0 R (nc/nc) =. n-c Negligible bleed at 0 C 0 0 First Peak Retention Meets Second Requirement Excellent Peak Symmetry This glass column is no longer available. Request the same dimensions in a fused silica column, as a custom column. Bulletin

14 Table. SIMDIS Data from Petrocol Capillary Columns and Packed Columns Show Good Agreement n Calc. Temp.( F) t Boiling Pt. ( F) s Sample Volume Petrocol 0 Packed Relative Petrocol ASTM Packed Relative % Off m Capillary Column Column Difference % Capillary Column Column Consensus Difference % IBP FBP.. Means for 0 injections. Each value represents % of the sample eluted from the column. Data obtained by using a commercial gasoline sample and Spectra-Physics D0 SIMDIS software. Data obtained by using ASTM reference gas oil sample and Spectra-Physics D SIMDIS software. Figure. Rapid Analysis of Reference Gas Oil with Negligible Bleed Column: Petrocol, m x 0.mm ID, 0.µm film : Oven: -0 C to 0 C at 0 C/min, hold min Carrier: nitrogen, ml/min. 0 C Inj.: 0.µL Reference Gas Oil ( ), direct injection, 0 C 0 0 For more information, request Bulletin. Negligible Bleed at 0 C -0 Aliphatics from Aromatics in Gasoline Use a WAX 0 column to separate aliphatic and aromatic hydrocarbons. Nonane will elute before benzene, and the xylene isomers will be resolved (Figures 0 and ). Figure 0. Aliphatics in Gasoline Elute Before Aromatics Column: WAX 0, 0m x 0.mm ID,.0µm film : -U Oven: 0 C ( min) to 0 C at C/min ( min hold) Carrier: helium, ml/min Inj.: 0.µL of a super unleaded gasoline C and Lighter Aliphatics Benzene Toluene Trace Aliphatics C0 Ethylbenzene C 0. p-xylene. m-xylene. Isopropylbenzene. o-xylene. n-propylbenzene. -Methyl--ethylbenzene.,,-Trimethylbenzene. -Methyl--ethylbenzene.,,-Trimethylbenzene 0.,,,-Tetramethylbenzene. Methylnaphthalenes. Dimethylnaphthalenes Naphthalene Analysis completed in less than 0 minutes Negligible Bleed at 0 C Bulletin

15 0 Figure. Nonane Elutes Before Benzene; Xylene Isomers Easily Resolved Column: WAX 0, 0m x 0.mm ID glass,.0µm film s Oven: 0 C Carrier: helium,.ml/min, 0 C Inj.: 0.µL CS containing % each component, direct injection Benzene n-nonane. n-hexane.,,-trimethylpentane U Unknown. n-octane. n-decane. Toluene. Ethylbenzene. Isopropylbenzene. n-propylbenzene Polynuclear Aromatic Hydrocarbons in Gasoline SPB columns offer the efficiency and high thermal stability you need to isolate individual PAHs in gasoline (Figure ). Figure. Gasoline Concentrated for Monitoring PAH Content Column: SPB-, 0m x 0.mm ID, 0.µm film : 0 Oven: C to 0 C at C/min Carrier: helium,.cm/sec Inj.: 0.µL unweathered and 0.0µL weathered super unleaded gasoline Unweathered Sample U x-xylene m-xylene p-xylene This glass column is no longer available. Request the same dimensions in a fused silica column, as a custom column, or request Cat No. 0-U (0 x 0.mm ID fused silica,.0µm film).. Naphthalene. -Methylnaphthalene. -Methylnaphthalene. Dimethylnaphthalenes. Acenaphthalene. Acenaphthene. Fluorene. Phenanthrene. Anthracene 0. Fluoranthene Front End Weathered PAH Region Concentrated Weathered Concentrated ,00 Bulletin

16 Trace Compounds in Petroleum Products Use a WAX 0 column to detect 0ppm or less of sulfolane in aromatic-rich or raffinate petroleum fractions, or in water washes (Figure ). Sulfolane is eluted after the dimethylnaphthalenes, ensuring minimal interference. A 0.mm ID WAX 0 capillary column (Figure ) can replace several packed columns for monitoring impurities in aromatic petroleum products (ASTM Method D). It ensures: Faster analyses Greater resolution More stable baseline for trace analyses Separation of xylene isomers Rapid recycle times Longer column life The wide bore WAX 0 column can be used with packed column injection and detector systems. Figure. Detect Low Levels of Sulfolane in Petroleum Streams Figure. Impurities in Aromatic Petroleum Products by ASTM Method D Major Component Overloaded to Detect Trace Components Column: Oven: Carrier: Det.: Inj.: WAX 0, 0m x 0.mm ID glass,.0µm film s 00 C (0 C for cyclohexane) helium,.cm/sec (.ml/min) FID, x 0 - AFS ( x 0 - AFS for cyclohexane) 0.µL each major aromatic compound containing 000ppm benzene, direct injection m-xylene. C/<C aliphatics. Benzene. Toluene. m-xylene. o-xylene Cyclohexane. Cyclohexane. Benzene Column: Oven: Carrier: Det.: Inj.: WAX 0, 0m x 0.mm ID glass,.0µm film s C to C at C/min helium,.cm/sec (ml/min) FID µl gasoline (w/o and w/0.0% sulfolane), direct injection Ethylbenzene. C/<C aliphatics. Benzene. C0 aliphatics. Toluene. Ethylbenzene Dimethylnaphthalenes Sulfolane, 0.0% Potential for interference with sulfolane is minimal Toluene. C/<C aliphatics. Benzene. Toluene Styrene. C/<C aliphatics. Benzene. C0 aliphatics. Styrene x 0 - AFS Sulfolane, 0.0% x 0 - AFS -00 This glass column is no longer available. Request the same dimensions in a fused silica column, as a custom column, or request Cat No. 0-U (0 x 0.mm ID fused silica,.0µm film) ,00,00,00,00 Bulletin

17 Miscellaneous Petrochemicals Analyses More Sample Information from Complex Hydrocarbon Mixtures Our nonpolar Petrocol DH 0. and SPB- capillary columns provide excellent resolution of complex mixtures such as charcoal lighter fluid, turpentine, and gasoline (Figures -). These bonded phase columns offer a potentially long column life. You can use 0.mm and 0.mm ID SPB- columns in packed column instruments, with thermal conductivity or other less sensitive detectors. These wide bore capillary columns will give you more sample information than any packed column can provide. Figure. Charcoal Lighter Fluid Column: Petrocol DH 0., 0m x 0.0mm ID, 0.0µm film : -U Oven: C ( min) to 00 C at C/min Carrier: helium, -cm/sec Figure. Gasoline on a Capillary Column in a Packed Column Chromatograph Column: Oven: Carrier: Det.: Inj.: 0 SPB-, 0m x 0.mm ID glass,.0µm film s C ( min) to 00 C at C/min, hold helium,cc/min (flow controlled) TCD 0.µL gasoline, direct injection. Air. Propane. Isobutane. n-butane. Isopentane. n-pentane. -Methylpentane. -Methylpentane. n-hexane 0. Methylcyclopentane.,-Dimethylpentane. Benzene. Cyclohexane. -Methylhexane. -Methylhexane. n-heptane.,-dimethylhexane. Toluene. -Methylheptane -Methylheptane 0. -Methylheptane. n-octane. Ethylbenzene. m-xylene p-xylene. -Methyloctane. -Methyloctane. o-xylene. n-nonane. Propylbenzene. n-decane 0. n-undecane. nc. nc0. nc. nc. nc. nc. nc. nc. nc Figure. Turpentine Column: Petrocol DH 0., 0m x 0.0mm ID, 0.0µm film : -U Oven: C ( min) to 00 C at C/min Carrier: helium, -cm/sec. nc. nc0. nc. nc. nc. nc. nc. nc. nc This glass column is no longer available. Request the same dimensions in a fused silica column, as a custom column, or request Cat No. (0 x 0.mm ID fused silica,.0µm film). Bulletin

18 Because our SPB- bonded phase columns are stable to 0 C, you can use them to resolve many high-boiling hydrocarbons (Figure ). To minimize column bleed and avoid splitter discrimination, use a 0.µm film, 0.mm ID column and cool on-column injection. Stabilized, high polarity Nukol columns provide sharp separation of oxo-isooctyl isomers for accurate determination of -ethylhexanol (Figure ). SPB-0 bonded phase columns resolve almost all alkylbenzene isomers to baseline (Figure 0). The Petrocol DH Octyl column completely separates methanol from isobutane (Figure ). Figure. -Ethylhexyl Alcohol Isolated from Other Oxo-isooctyl Alcohol Isomers Column: Nukol, 0m x 0.mm ID, 0.µm film : Oven: 0 C to 0 C at C/min (hold min) Carrier: helium, 0cm/sec ( x 0 - AFS) Inj.: 0.µL CH OH, % isooctyl alcohols Methanol (Diluent) -Ethylhexyl Alchol Figure. Petroleum Wax Components Resolved Without Splitter Discrimination Column: SPB-, 0m x 0.mm ID, 0.0µm film : Oven: 00 C to 0 C at C/min (hold min) Carrier: helium, 0mL/min Inj.: 0µg petroleum wax in µl n-dodecane Oxo-isooctyl Alcohols. C. C0. C. C0. C Figure 0. Complex Mixtures of Alkylbenzenes Column: SPB-0, 0m x 0.mm ID, 0.µm film : 0 Oven: 0 Carrier: helium, 0cm/sec Inj.: 0.µL of mixed alkylbenzenes, split 00: øc0*. øc0. øc0. øc0. øc. øc. øc. øc. C0 tetralins 0. øc. øc. øc. øc. øc. C tetralins. øc. øc, øc. øc. øc 0. øc. C tetralins. øc. øc. øc. øc. øc. øc * Position of phenyl group, chain length Bulletin

19 Figure. Methanol and Isobutane Column: Petrocol DH Octyl, 00m x 0.mm ID, 0.µm film : Oven: C Carrier: helium, cm/sec, 0 C Inj.: 0.µL, split :, 0 C. Methanol. Isobutane Capillary Column Improves Analyses of Dodecylphenol Product Our 0.mm ID SPB- fused silica capillary column is ideal for this purpose. Compared to packed columns, the inert, bonded phase capillary column improves peak symmetry for the acidic sample components. Phenol elutes before the C olefins, rather than after them, for more reliable quantification of phenol and olefins (Figure ). This wide bore capillary column can be used in packed column instruments. 0-0 Figure. Docecylphenol Product Components Separate Well on a Bonded Phase Capillary Column Column: SPB-, 0m x 0.mm ID,.µm film : 0-U Oven: 0 C ( min) to 00 C at C/min Carrier: helium, ml/min, C Inj.: 0.µL carbon disulfide:dodecylphenol product (:), direct injection, 00 C Phenols/Polymers Cresol Isomers Using an Acidic, Bonded Phase Column Phenols (and many other acidic polar compounds) are eluted from high efficiency Nukol bonded phase capillary columns as sharp, symmetrical peaks. A Nukol column will resolve p-cresol from the dimethylphenols (Figure ).. CS (solvent). Phenol. C olefins. Alkylphenol ethers. Dodecylphenol. Di-dodecylphenol Figure. Resolve p-cresol from Dimethylphenols Column: Nukol, 0m x 0.mm ID, 0.µm film : 0 Oven: 00 C Carrier: helium, 0cm/sec Inj.: µl methylene chloride containing µg each phenol, split 00: ,- &,-Dimethylphenol p-cresol.,-dimethylphenol. o-cresol. Phenol. o-ethylphenol. m-cresol.,-dimethylphenol. p-ethylphenol &,-Dimethylphenol. m-ethylphenol.,-dimethylphenol Bulletin

20 Solvents Analyze Almost Any Combination of Solvents You can easily separate complex mixtures of common solvents on our nonpolar SPB- or polar WAX 0 capillary column. (SPB- columns separate the alcohols by boiling point.) Compare the separations shown in Figures - to determine which column is best for your samples. Furthermore, you can use 0.mm and 0.mm ID columns in systems designed for packed columns. Figure. Industrial Solvents Column: SPB-, 0m x 0.mm ID,.0µm film : 0 Oven: 0 C ( min) to C at C/min, hold min Carrier: helium, cm/sec (set at 0 C), 0 C Inj.: 0.µL approximately equal proportions of each component, split 00:, 0 C 0, U Figure. Solvents on Capillary Column in Packed Column GC Column: SPB-, 0m x 0.mm ID,.0µm film : 0 Oven: 0 C ( min) to C at C/min (hold) Carrier: helium, 0cm/sec Inj.: 0.µL, approx. equal proportions of each component, direct injection 0,, 0,U 0, U U 0,,, Figure. Solvents on Capillary Column in Packed Column GC Column: WAX 0, 0m x 0.mm ID,.0µm film : 0-U Oven: 0 C ( min) to C at C/min (hold min) Carrier: helium, ml/min (flow controlled) Inj.: 0.µL mixed neat solvents, direct injection Solvents for Figures -. Methanol. Methyl formate. Ethanol. Acetone. Isopropyl alcohol. Ethyl formate. Methylene chloride. n-propyl alcohol. Methyl ethyl ketone 0. sec-butyl alcohol. Ethyl acetate. Chloroform. Tetrahydrofuran. Methyl Cellosolve. Isobutyl alcohol.,,-trichloroethane. Isopropyl acetate. n-butyl alcohol. -Nitropropane 0.,-Dioxane. Ethyl Cellosolve U Unknown. Methyl isobutyl ketone. Toluene. Isobutyl acetate. Mesityl oxide. n-butyl acetate. Diacetone alcohol. -Methyl--hexanone. Ethylbenzene 0. m-xylene. p-xylene. Cyclohexanone U Unknown. o-xylene. Cellosolve acetate. Butyl Cellosolve. Isoamyl acetate,,,, U, U -0 0 Bulletin

21 Eliminate Glycol Peak Tailing and Sample Decomposition Use 0.mm ID Nukol columns to analyze these highly polar compounds with good resolution and peak symmetry, and at low temperatures that eliminate decomposition (Figure ). Capillary Chromatography for Glycols in a Packed Column Chromatograph You can have the inertness and high resolution of capillary GC without using expensive, specialized equipment. Use 0.mm ID Nukol columns in packed column chromatographs, with injectors and detectors designed for packed columns. Analyze Trace Solvents in Pharmaceutical Products Figure shows the elution of six volatile organic solvents, commonly used in manufacturing pharmaceuticals, separated on a thick film SPB- capillary column. The low parts-per-million concentrations are representative of residual solvent levels in bulk drug preparations. Figure. Solvents Commonly Used in Pharmaceuticals Manufacture Column: SPB-, 0m x 0.mm ID, µm film : Oven: 0 C ( min) to 00 C at 0 C/min Carrier: helium, -cm/sec, 0 C Inj.:.0cc, split 00:, 00 C Component Concentration (ppm) s. Ethylene chloride 0. Methylene chloride 0. Chloroform 0. Benzene 0.,-Dioxane 0. Trichloroethylene Figure. Nukol Column Provides Sharp Glycol Peaks Column: Nukol, 0m x 0.mm ID, 0.µm film : Oven: C ( min) to 0 C at 0 C/min, hold min Carrier: helium, 0cm/sec (set at C), 0 C Inj.: 0.0µL ink solvents mixture, direct injection, 0 C. Heptane. Ethyl acetate. Isopropyl acetate. Methanol. Isopropanol. Ethanol. n-propyl acetate. n-propanol. Carbitol U Unknown 0. Ethylene glycol. Diethylene glycol U Values are equivalent to headspace analysis for 0g of a pharmaceutical product. Impurities in Solvents For monitoring impurities in ketones (ASTM Methods D0, D, and D) and -ethylhexanol, WAX 0 and Nukol capillary columns improve peak resolution, shorten analysis times, and provide more repeatable results than packed columns (Figures to ). Figure. Trace Levels of Impurities in Methyl Ethyl Ketone by Capillary GC Column: WAX 0, 0m x 0.mm ID, 0.µm film : 0 Oven: 0 C ( min) to 00 C at C/min Carrier: helium, cm/sec, 0 C Inj.: 0.µL MEK, split 00: Methyl ethyl ketone Contaminants, 0.0% each. Diisopropyl ether. Dipropyl ether. sec-butyl ether*. Acetone. -Octene. Ethyl acetate. Methanol. Isopropanol. Ethanol 0. tert-butanol. sec-butanol. n-propanol 0 Bulletin 0 *Contaminant in sec-butanol, not spiked in sample. -0

22 Figure 0. Choose Maximum Resolution or Save Time When Monitoring Purity of Methyl Isobutyl Ketone Column: WAX 0, 0m x 0.mm ID, 0.µm film : 00-U Oven: 0 C Carrier: helium, cm/sec Inj.: 0.µL MIBK, split 00: Fastest Analysis 0.mm ID Column Figure., Methyl isobutyl ketone Contaminants, 0.0% each. Acetone. -Propanol. -Pentanone. -Pentanone. α-mesityl oxide. β-mesityl oxide. Methyl isobutyl carbinol Maximum Resolution 0.mm ID Column 0 0 Impurities from -Ethylhexanol Columns: 0m x 0.mm ID, 0.µm film : 00-U; Oven: 0 C Carrier: helium, 0cm/sec Inj.: 0.µL -ethylhexanol, split 00:, Methyl isobutyl ketone -0,00 Table. Capillary Column Ensures Consistent Measurements of Impurities in Solvents Variation in Measurement (% absolute) Column - Solvent/Impurity Injection* - Injection** Lab* - Lab** Column MIAK Isopropyl alcohol Mesityl oxide Methyl isoamyl carbitol Total ketones MAK Isopropyl alcohol Methyl isobutyl carbitol Total ketones * Value for WAX 0 capillary column. ** ASTM specification for packed columns. Packed column specifications established at 0.% each impurity, capillary column values determined at 0.0% each impurity. High Resolution or High Speed Analyses When you need high resolution or a fast separation, a 0.0mm ID WAX 0 capillary column is ideal. Its high efficiency (approximately % greater than that of a 0.mm ID column) lets you obtain the greatest resolution possible. When analysis time is most important, you can trade efficiency for more rapid separations (Figures and ). Figure. Trace Contaminants Completely Resolved from Major Sample Component Column: WAX 0, 0m x 0.0mm ID, 0.0µm film : Oven: 0 C Carrier: helium, cm/sec Inj.: 0.µL p-xylene product, split (0:). Ethylbenzene. Toluene. p-xylene. m-xylene. o-xylene WAX 0 Column Nukol Column -Ethylhexanol -Ethylhexanol Contaminants, 0.0% each. -Pentanol (int. std.). -Ethyl--methyl--pentanol p-xylene and m-xylene resolved to baseline in less than minutes, ,0 Bulletin

23 Figure. Fastest Separation of Aromatic Compounds in Balanced Proportions Column: WAX 0, 0m x 0.0mm ID, 0.0µm film : Oven: C Carrier: helium, cm/sec Inj.: 0.µL hexane (.% each analyte), split (0:) Because aromatic and nonaromatic compounds have similar boiling points, they are hard to resolve on nonpolar columns. Therefore, analysis of aromatics in gasoline has been a difficult application. Using a narrow bore polar capillary column simplifies quantitative analysis of aromatic compounds. When gasoline is analyzed on a polar 0m x 0.0mm ID WAX 0 column, benzene and higher molecular weight aromatics are retained until after most nonpolar components (up to n-nonane) elute (Figure ).. Benzene. Toluene. Ethylbenzene. p-xylene. m-xylene. o-xylene p-xylene and m-xylene resolved to baseline in less than minutes 0 - Figure. Monitor Aromatic Components of Gasoline Rapidly and Efficiently Column: WAX 0, 0m x 0.0mm ID, 0.0µm film : Oven: 0 C ( min) to 0 C at C/min Carrier: helium, cm/sec Inj.: 0.µL gasoline, split (0:) 0,, 0,, 0,. Benzene. Toluene C Aromatics. Ethylbenzene. p-xylene. m-xylene. o-xylene C Aromatics. Isopropylbenzene. n-propylbenzene. -Methyl--ethylbenzene 0. -Methyl--ethylbenzene.,,-Trimethylbenzene. -Methyl--ethylbenzene.,,-Trimethylbenzene.,,-Trimethylbenzene. Indane C0 Aromatics. tert-butylbenzene. Isobutylbenzene. sec-butylbenzene. -Methyl--isopropylbenzene 0. -Methyl--isopropylbenzene.,-Diethylbenzene. -Methyl--isopropylbenzene. -Methyl--n-propylbenzene. -Methyl--n-propylbenzene.,-Diethylbenzene. n-butylbenzene.,-dimethyl--ethylbenzene.,-diethylbenzene. -Methyl--n-propylbenzene 0.,-Dimethyl--ethylbenzene.,-Dimethyl--ethylbenzene.,-Dimethyl--ethylbenzene.,-Dimethyl--ethylbenzene.,-Dimethyl--ethylbenzene.,,,-Tetramethylbenzene.,,,-Tetramethylbenzene.,,,-Tetramethylbenzene Polynuclear Aromatics. Naphthalene. -Methylnaphthalene 0. -Methylnaphthalene. Dimethylnaphthalenes 0 Analysis Completed in Less Than 0 utes Bulletin

24 Aromatics / Aliphatics by Packed Column GC Aliphatics from Aromatics Most packed columns allow aliphatics and aromatics to elute together. Our high polarity,,-tris (-cyanoethoxy)propane (TCEP), BC-0, and BC-0 packings retard the aromatics relative to the aliphatics (Figure ). The BC-0 phase has a higher temperature limit (0 C) than the BC-0 or TCEP phases ( C). Figure. Xylene Isomers Packing: GP % SP-00/.% Bentone on 00/0 PORT : (0g)/bottle Column: ' x /" stainless steel : (general configuration stock column, other stock columns available) Oven: C Carrier: nitrogen, 0mL/min Inj.: 0.0µL, approx. equal volumes each component Xylene Isomers % SP-00/% Bentone packing separates o-, m-, and p- xylene and ethylbenzene in less than minutes. If styrene, isopropylbenzene, or n-propylbenzene are present, use % SP-00/.% Bentone (Figure ).. Benzene. Toluene. Ethylbenzene. p-xylene. m-xylene. o-xylene. Isopropylbenzene. Styrene. n-propylbenzene Difficult-to-Resolve Aromatics Both Carbopack F-TA and Carbopack C/0.% SP-000 columns will rapidly separate butylbenzenes and many other difficult-toresolve isomers. Analyses on a Carbopack F-TA column require half as much time (Figure ). Figure. Aromatics and Aliphatics Packing: GP 0% TCEP on 00/0 Chromosorb P AW : 0-U (packing, 0g/bottle) Column: ' x /" stainless steel Oven: 0 C Carrier: nitrogen, 0mL/min Inj.: 0.µL, approx. equal volumes each analyte. n-hexane. n-heptane. n-octane. n-nonane. n-decane. Benzene. Toluene. Ethylbenzene. m-xylene 0. p-xylene. Isopropylbenzene. o-xylene 0 Figure. Analyses on Carbopack F-TA Column Require Half the Time : Column: : Carbopack F Column 0 (packing, g/bottle) ' x /" ID stainless steel -U (general configuration stock column, other stock columns available) Oven: C Carrier: nitrogen, 0mL/min Inj.: µl chloroform (~0.% each analyte) Carbopack C Column -0,0. tert-butylbenzene. sec-butylbenzene. Isobutylbenzene. n-butylbenzene ,-0 Bulletin

25 Aromatics/Aliphatics by Capillary GC The Petrocol DH Octyl capillary column (Figure ) offers complete baseline resolution of the BTEX compounds, including the difficult-to-separate p- and m-xylene isomers. Figure. BTEX Compounds Column: Petrocol DH Octyl, 00m x 0.mm ID, 0.µm film : Oven: C ( min) to 00 C at C/min ( min) Carrier: helium, cm/sec, 0 C Inj.: 0.µL, split :, 0 C. Benzene. Toluene. Ethylbenzene. p-xylene. m-xylene. o-xylene Figure. C-C Saturated and Unsaturated Hydrocarbons Packing: % SP-00 on 0/00 Chromosorb P AW : (g) (packing only) Column: 0' x /" stainless steel : 0-U (general configuration column) Oven: 0 C Carrier: helium, ml/min Inj.: 0.µL ASTM Section L Blend No., plus Cs. Ethane. Propane. Propylene. Isobutane. n-butane. -Butene/Isobutylene. trans--butene. cis--butene. Isopentane 0.,-Butadiene. n-pentane. -Pentene. -Methyl--butene/trans--Pentene. cis--pentene. -Methyl--butene. -Methyl--pentene. -Methyl--pentene. n-hexane U Unknown. -Methylhexane U C-C Hydrocarbons by Packed Column GC Figure 0. C Unsaturates Packings Specifically Prepared for Optimum Hydrocarbon Separations % SP-00 columns offer excellent resolution of concentrated C-C hydrocarbons (Figure ). Use these extremely durable columns between 0 C and 0 C, with sample valves or direct injections. Carbopack C/0.% picric acid packing is prepared specifically for separating C compounds or C unsaturates (Figure 0). Use this packing at temperatures to 0 C. Packing: GP 0/00 Carbopack C/0.% picric acid : (g) Column: m x /" stainless steel : (general configuration column) Oven: 0 C Carrier: nitrogen, 0mL/min Inj.: µl ASTM Section L Blend No. Component & Concentration (Mole %). Propane.0. Propylene 0.. Isobutane.. -Butene.0. n-butane.0. Isobutylene.0. cis--butene.0. trans--butene.0.,-butadiene.0 0. Isopentane.. n-pentane 0., Bulletin

26 Polymers by Gel Permeation Chromatography Silica-Based Columns By combining a 00Å pore and a 00Å pore SIL column in series, you can separate compounds having molecular weights from 00 to over 00,000 daltons (Figure ). SIL LC-Diol columns are suitable for separating watersoluble synthetic polymers, or for normal phase analyses (Figure ). Figure. Polystyrene Standards by Molecular Weight Column : SIL LC-, cm x.mm ID, µm particles* : Column : SIL LC-0, cm x.mm ID, µm particles, 00Å pores* : Mobile Phase: tetrahydrofuran Flow Rate: ml/min Temp.: C Det.: UV, nm Inj.: 0µL, polymer quantities listed.,00,000 (.µg). 00,000 (.µg). 00,000 (.µg).,000 (.0µg).,00 (.µg).,000 (.µg).,000 (0.0µg). Toluene (.µg) Low Molecular Weight Compounds by Gas-Solid Chromatography Porous Polymers for Highly Consistent Performance HayeSep porous polymers are thoroughly cleaned, then conditioned for hours under oxygen-free nitrogen before they are packaged. This careful preparation ensures materials that are highly consistent, with no shrinkage and minimum bleed. And they will perform more consistently than any other commercial polymer you are currently using (Figures -). HayeSep N, P, Q, R, S, and T polymers are interchangeable with the corresponding Porapak polymers for separating low molecular weight mixtures containing halogenated or sulfur-containing compounds, water, alcohols, glycols, free fatty acids, esters, ketones, or aldehydes. HayeSep A polymer separates permanent gases (hydrogen, nitrogen, oxygen, argon, carbon monoxide, nitric oxide) at ambient temperatures. Use it at higher temperatures to analyze C hydrocarbons, hydrogen sulfide, or water. HayeSep B polymer is designed to separate C and C amines, and trace levels of ammonia and water. It does not require caustic washes before it is packed into a column. HayeSep C polymer is for analyses of polar molecules such as hydrogen cyanide, ammonia, hydrogen sulfide, and water. Separation characteristics are similar to those of Chromosorb 0 polymer. HayeSep D polymer exhibits superior separation characteristics for light gases. It can be used to isolate carbon monoxide and carbon dioxide from room air at ambient temperatures, or to elute acetylene before other C hydrocarbons. It is particularly useful for separations and analyses of water and hydrogen sulfide. *Columns in series Figure. Hydrocarbons and Sulfur Gases on HayeSep Q Figure. Nonionic Surfactants Resolved Almost to Baseline (Normal Phase Analysis) Column: SIL LC-Diol, cm x.mm ID, µm particles : 0 Mobile Phase: A = hexane:methylene chloride (:) B = hexane:methylene chloride:methanol (0:0:0) Flow Rate: ml/min Temp.: C Det.: UV, 0nm Inj.: 0µL methylene chloride containing 0µg surfactant -0 Ethoxy Groups (Octylphenol) Gradient Program Time (min) %B Ethoxy Groups (Nonylphenol) Packing: HayeSep Q : 00-U (packing) Column: ' x /" Oven: 0 C Carrier: helium, 0cc/min. Air. Methane. Carbon dioxide. Ethylene. Acetylene. Ethane. Hydrogen sulfide. Carbonyl sulfide. Propylene 0. Propane. Propadiene ,0 0-0 Bulletin

27 Figure. Mapp Gas on HayeSep R Packing: HayeSep R : 00 (packing) Column: 0' x /" stainless steel Oven: 0 C Carrier: helium, 0mL/min Inj.: ml Figure. Trace Ammonia in Water on HayeSep C Packing: 0/00 HayeSep C : 0 Column: ' x /" Oven: C Det.: TCD Inj.: µl Figure. Scott Mix on HayeSep D Packing: HayeSep D : 0 (packing) Column: 0' x /" Ni Oven: 0 C ( min) to 0 C at C/min Carrier: helium, 0mL/min Det.: P.E. 00 T.C., ma, 0 C Inj.: 00mL, Valco valve, ambient. Air. Methane. Carbon dioxide. Ethane. Propylene. Propane. Propadiene. Methyl acetylene. Air. Carbon dioxide. Ammonia (00ppm). Water (balance). Nitrogen (balance). Carbon monoxide, %. Methane, %. Carbon dioxide, %. Acetylene, %. Ethylene, %. Ethane, % Figure. Permanent Gases on HayeSep A Packing: 0/00 HayeSep A : 0 (packing) Column: ' x /" Oven: C Carrier: helium, cc/min Det.: P.E. 00 T.C, ma Inj.: ml air plus hydrogen and carbon monoxide. Hydrogen %. Nitrogen.%. Oxygen %. Argon 0.%. Carbon monoxide % Table. Characteristics of HayeSep Polymers Tapped HayeSep Maximum Operating Surface Area Bulk Density Polarity Polymer Temp. ( C) (m /gram) (gram/cc) ( = lowest; 0 = highest) A 0. B C 0 0. D 0 0. N 0 0. P Q 0. R 0 0. S 0 0. T Bulletin

28 Ordering Information: Capillary Columns for Petrochemical Analysis Stationary Phase Operating Temperature Dimensions Carbowax Amine 0 to 0 C m x 0.mm ID,.0µm film 0m x 0.mm ID,.0µm film 0m x 0.mm ID,.0µm film PTA- -0 C to 0 C 0m x 0.mm ID, 0.µm film Bentone /DnDP amb. to0 C m x 0.mm ID 0 (SCOT) 0m x 0.mm ID 0 m x 0.mm ID 0-U 0m x 0.mm ID 0 m x 0.mm ID 00-U 0m x 0.mm ID 0-U Petrocol DH -0 to 0 C 00m x 0.mm ID, 0.µm film 0-U Petrocol DH to 0 C 0m x 0.0mm ID, 0.µm film -U Petrocol DH 0-0 to 0 C 0m x 0.mm ID,.0µm film Petrocol DH Octyl -0 to 0 C 0m x 0.0mm ID, 0.µm film 00m x 0.mm ID, 0.µm film 0m x 0.mm ID,.0µm film 0 Petrocol (" cage) subamb. to 0 C m x 0.mm ID, 0.µm film Petrocol (" cage) m x 0.mm ID, 0.µm film 0-U Petrocol EX subamb. to 0 C m x 0.mm ID, 0.µm film SPB- -0 to 0 C 0m x 0.mm ID, 0.µm film 0m x 0.mm ID, 0.µm film -U SPB- Thin Film -0 to 0 C m x 0.mm ID, 0.µm film 0 0m x 0.mm ID, 0.µm film SPB- SULFUR -0 to 0 C 0m x 0.mm ID,.0µm film TCEP subamb. to C 0m x 0.mm ID, 0.µm film 0m x 0.mm ID, 0.µm film SCOT Capillary Columns Support-coated open tubular (SCOT) columns are prepared by depositing a layer of liquid phase-coated support particles on the inner wall of the tubing. This technology, developed by Perkin- Elmer, makes available many phases that cannot be obtained on conventional (wall-coated open tubular) capillary columns. SCOT columns combine the advantages of capillary GC (sensitivity and excellent sample resolution) with the extensive stationary phase library of packed column GC. Stainless Steel SCOT Columns 0' x /'' OD x 0.0'' ID with /'' connections Max. Temp. Liquid Phase ( C) Beta Bentone /DNDP 0 MBMA Squalane 0 0 TCEP 0 0 Di-n-decylphthalate Bulletin

29 Developed for Other Appliciations Additional Information amines/amino-containing pharmaceuticals volatile basic compounds Reporter XI, No., pp - and other basic compounds amines and other volatile basic analytes BTEX purity analyses of aromatics complex hydrocarbon mixtures (e.g., gasoline) solvent purity, Bulletin hydrocarbons (gases, complex mixes), ASTM D impurities in Reporter XI, No., pp - ASTM D monomers and additives Application Notes and petroleum products Reporter X, No., pp 0- Reporter XIII, No., pp - SIMDIS (simulated distillation) Bulletin (ASTM Method D) fingerprinting for forensics; Reporter X, No., pp -, SIMDIS (extended ASTM Method D) solvents, Bulletin SIMDIS general purpose nonpolar column Reporter XII, No., pp - oxygenates in gasoline (ASTM D) general purpose nonpolar column Publication 0 paraffin mixtures, triglycerides, other high temperature Reporter IX, No., pp - other high temperature analyses analyses Reporter X, No., pp - sulfur compounds stack gas analysis, Bulletin (as gases or in complex hydrocarbon mixes) light hydrocarbons (gases) aromatics in mineral spirits Reporter XIII, No., pp. 0- oxygenates in gasoline (ASTM D) Publication 0 Bulletin

30 Columns: Special Purpose PLOT Mol Sieve A PLOT Like their packed column counterparts, Mol Sieve A PLOT columns separate permanent gases. Oxygen, nitrogen, carbon monoxide and methane can be separated in less than minutes. More difficult separations, such as argon from oxygen, can be achieved by using subambient temperatures. A proprietary procedure fixes the molecular sieve to the fused silica tubing and ensures the particles will not be dislodged from the tubing in normal use. The 0.mm ID column can be used in packed column chromatographs. Dimensions Max. Temp. (fused silica) ( C) 0m x 0.mm ID 00 0m x 0.mm ID 00 Supel-Q PLOT Supel-Q TM PLOT columns contain a porous divinylbenzene polymer that effectively resolves carbon dioxide and C-C hydrocarbons at above ambient temperatures. It also is suitable for analyses of other gases, such as sulfur gases, and alcohols, ketones, aldehydes, and many polar compounds. Gasoline and other petroleum fractions can be analyzed as well. These columns exhibit very little bleed, even at the maximum temperature. Relative to packed columns (e.g., Porapak-Q), Supel-Q PLOT columns offer better resolution in less time. A proprietary procedure fixes the polymer to the fused silica tubing and ensures the particles will not be dislodged from the tubing in normal use. The 0.mm ID column can be used in packed column chromatographs. Dimensions Max. Temp. (fused silica) ( C) 0m x 0.mm ID 0 0m x 0.mm ID 0 Carboxen-00 PLOT Carboxen TM -00 porous layer open tubular (PLOT) columns separate permanent gases and C, C, and C light hydrocarbons, using above-ambient initial temperatures. They also are ideal for resolving formaldehyde/water/methanol (formalin) mixtures and monitoring impurities in ethylene. A proprietary procedure fixes a porous carbon molecular sieve (surface area > 0m²/gram) to fused silica tubing and ensures the carbon layer will not be dislodged from the tubing in normal use. Use Carboxen-00 columns with high flow rates and rapid temperature programs, up to 0 C, to ensure excellent, fast separations. The 0.mm ID column can be used in packed column chromatographs. Carboxen-00 PLOT A carbon molecular sieve column for separating the permanent gases: hydrogen, nitrogen, carbon monoxide, methane, carbon dioxide, C and C hydrocarbons. Oxygen is separated from nitrogen. The 0.mm ID columns can be used in packed column chromatographs. Manufactured under US patents,, and,0,0. Dimensions Max. Temp. (fused silica) ( C) 0m x 0.mm ID 0 0m x 0.mm ID 0 Dimensions Max. Temp. (fused silica) ( C) 0m x 0.mm ID 0 -U 0m x 0.mm ID 0 Patent pending. 0 Bulletin

31 Columns and Packings, Listed by Application Detailed Hydrocarbons Analyses Petrocol DH Fused Silica Capillary Column 00m x 0.mm ID, 0.0µm film 0-U Petrocol DH 0. Fused Silica Capillary Column 0m x 0.0mm ID, 0.0µm film -U Petrocol DH 0 Fused Silica Capillary Column 0m x 0.mm ID,.0µm film Petrocol DH Octyl Fused Silica Capillary Columns 00m x 0.mm ID, 0.µm film 0m x 0.mm ID,.0µm film 0 Light Hydrocarbons SPB- Thick Film Fused Silica Capillary Column 0m x 0.mm ID, µm film SPB- Thick Film Fused Silica Capillary Column 0m x 0.mm ID, µm film High Boiling Hydrocarbons SPB- Thin Film Fused Silica Capillary Columns m x 0.mm ID, 0.0µm film 0 0m x 0.mm ID, 0.0µm film Oxygenates Petrocol DH Octyl Fused Silica Capillary Columns 00m x 0.mm ID, 0.µm film 0m x 0.mm ID,.0µm film 0 Petrocol DH 0 Fused Silica Capillary Column 0m x 0.mm ID,.0µm film Simulated Distillation By Packed Column GC Petrocol A Column 0 x / stainless steel Petrocol B Column 0 x / stainless steel Simulated Distillation By Capillary GC Petrocol Capillary Column m x 0.mm ID fused silica, 0.µm film Aliphatics from Aromatics in Gasoline WAX 0 Wide Bore Capillary Column 0m x 0.mm ID,.0µm film -U Polynuclear Aromatic Hydrocarbons in Gasoline SPB- Fused Silica Capillary Column 0m x 0.mm ID, 0.µm film 0 Trace Compounds in Petroleum Products WAX 0 Wide Bore Capillary Column 0m x 0.mm ID,.0µm film -U Miscellaneous Petrochemicals Analyses Petrocol DH 0. Fused Silica Capillary Column 0m x 0.0mm ID, 0.0µm film -U SPB- Capillary Columns 0m x 0.mm ID glass,.0µm film 0-U 0m x 0.mm ID fused silica, 0.0µm film SPB-0 Fused Silica Capillary Column 0m x 0.mm ID, 0.µm film 0 Nukol Fused Silica Capillary Column 0m x 0.mm ID, 0.0µm film Petrocol DH Octyl Fused Silica Capillary Columns 00m x 0.mm ID, 0.µm film 0m x 0.mm ID,.0µm film 0 Phenols / Polymers Nukol Fused Silica Capillary Column 0m x 0.mm ID, 0.µm film 0 SPB- Fused Silica Capillary Column 0m x 0.mm ID,.µm film 0-U Solvents SPB- Capillary Columns 0m x 0.mm ID fused silica,.0µm film 0-U 0m x 0.mm ID glass,.0µm film 0-U WAX 0 Capillary Columns 0m x 0.mm ID fused silica,.0µm film 0-U 0m x 0.mm ID glass,.0µm film Nukol Wide Bore Capillary Column 0m x 0.mm ID, 0.µm film SPB- Thick Film Fused Silica Capillary Columns 0m x 0.mm ID, µm film 0m x 0.mm ID, µm film -U Impurities in Solvents WAX 0 Fused Silica Capillary Columns 0m x 0.mm ID, 0.µm film 00-U 0m x 0.mm ID, 0.µm film 0 m x 0.mm ID, µm film 00-U Nukol Fused Silica Capillary Column 0m x 0.mm ID, 0.µm film High Resolution or High Speed Analyses WAX 0 Fused Silica Capillary Columns m x 0.0mm ID, 0.0µm film 0m x 0.0mm ID, 0.0µm film 0m x 0.0mm ID, 0.0µm film 0 Aromatics / Aliphatics by Packed Column GC Packings (0g) GP 0% TCEP on 00/0 Chromosorb P AW 0-U GP 0% TCEP on 0/00 Chromosorb P AW GP % BC-0 on 00/0 Chromosorb P AW DMCS GP % BC-0 on 00/0 Chromosorb P AW DMCS 0-U 0% SP -00 on 00/0 PORT 0% SP-00 on 0/00 PORT 0 GP % SP-00/.% Bentone on 00/0 PORT GP % SP-00/% Bentone on 00/0 PORT Aromatics / Aliphatics by Packed Column GC Packings 0/00 Carbopack C/0.% SP-000 g 0 g 0 0/0 Carbopack F-TA packing is available in packed columns only. C-C Hydrocarbons by Packed Column GC % SP-00 on 0/00 Chromosorb P AW packing in a 0 x / stainless steel column, general configuration ( coil, arms) 0-U Inquire about other configurations or lengths. Packings % SP-00 on 0/00 Chromosorb P AW (g) (-g fills a 0 x / column) GP 0/00 Carbopack C/0.% picric acid (g) Bulletin

32 Low Molecular Weight Compounds by Gas-Solid Chromatography HayeSep Polymers HayeSep porous polymers are thoroughly cleaned and conditioned before they are packaged, which ensures high consistency, and minimal shrinkage and bleed. Uses include the analysis of low molecular weight halogenated and sulfur-containing compounds, water, alcohols, glycols, free fatty acids, esters, ketones and aldehydes. These polymers also are used for separating hydrocarbons, ammonia, and other solvents. (Request HayeSep Application Bulletin available from Supelco). HayeSep A Copolymer of high-purity divinylbenzene and ethylene glycol dimethacrylate. Separates permanent gases at ambient temperatures. Use at higher temperatures to analyze C hydrocarbons, hydrogen sulfide, and water. HayeSep B Copolymer of divinylbenzene and polyethyleneimine. Separates C and C amines and trace levels of ammonia and water. HayeSep C USP code [S0]. Copolymer of divinylbenzene and acrylonitrile. For the analysis of polar molecules. Separation characteristics are similar to those of Chromosorb 0. HayeSep D High-purity divinylbenzene polymer. Exhibits superior separation characteristics for light gases, carbon monoxide, carbon dioxide, and acetylene (ahead of other C hydrocarbons). For the analysis of water and hydrogen sulfide. HayeSep N Copolymer of divinylbenzene and ethylene glycol dimethacrylate. HayeSep P Copolymer of divinylbenzene and styrene. HayeSep Q USP code [S]. Divinylbenzene polymer. HayeSep R USP code [S]. Copolymer of divinyl-benzene and N-vinyl-- pyrollidone. HayeSep S USP code [S]. Copolymer of divinyl-benzene and -vinyl-pyridine. HayeSep T Polymer of ethylene glycol dimethacrylate. Accessories for Analyzing High Boiling Hydrocarbons (page ) Cool On-Column Injection Sleeve Kit 0 Hamilton 0 Syringe With fixed needle Polywax x ml HayeSep Porous Polymers (cc bottles) HayeSep Mesh Max. Cat. Series Size Use Temp. ( C) No. HayeSep A 0/0 Permanent Gases 0 0/ /0 0 HayeSep B 0/0 C-C Amines, 0 0 0/00 Ammonia in H O 0 00/0 0 HayeSep C 0/0 Polar Compounds 0 0 0/ /0 00 HayeSep D 0/0 Light gases, CO, CO 0 0 0/ /0 0 HayeSep N 0/0 Acetylene, Ethylene 0 0/ /0 0 HayeSep P 0/0 Ammonia, 0 0 0/00 Water Alcohols 0 00/0 0 HayeSep Q 0/0 Hydrocarbons, 000-U 0/00 Sulfur Gases, 00-U 00/0 General 00 HayeSep R 0/0 Light Hydrocarbons, /00 Chlorinated 00 00/0 Compounds 00-U HayeSep S 0/0 C-C Hydrocarbons /00 Polar Compounds 00 00/0 00 HayeSep T 0/0 Light Hydrocarbons 00 0/00 Formaldhyde 00 00/0 0 Polymers by Gel Permeation Chromatography Analytical-Scale SIL Silica-Based Size Exclusion HPLC Columns Cartridge with Fittings Column.mm ID.0mm ID.0mm ID Phase Length Cartridge Cartridge Cartridge Particle, Pore Size (cm) LC- µm, 0Å -C0 -C0 0-U 0-C0 0-C0 -C0 -C0 LC-Diol µm, 0Å 0 0-C0 0-C0 Preparative-Scale SIL Silica-Based Size Exclusion HPLC Columns, cm x.mm µm silica, 00Å pores SIL LC-Si SIL LC- SIL LC-Diol µm silica, 00Å pores SIL LC-Si SIL LC-0 SIL LC-Diol Bulletin

33 Quantitative Reference Standards For P-N-A, P-O-N-A, P-I-A-N-O Analyses Use these mixes to determine retention times and indices and monitor response factors for components of complex mixtures of hydrocarbons. These standards are complex mixes of known quantities of hydrocarbons, accurately prepared by weight to three decimal places. Typical values are listed here; exact values will differ slightly from lot to lot. Formulations are weight percent. Each mix includes a detailed data sheet listing components by weight percent, mole percent, and liquid volume percent, retention times and retention indices for each component, and other information. A chromatogram from a 00-meter Petrocol DH capillary column (including conditions) is provided. Products are supplied in crimp-top vials with hole caps and septa. The shelf life of the unopened, refrigerated mixes is year. n-paraffins Mix n-paraffins (typical values shown). n-pentane,.% n-undecane,.% n-hexane, 0.% n-dodecane,. % n-heptane,.% n-tridecane,.% n-octane, 0.0% n-tetradecane,.0% n-nonane,.0% n-pentadecane,.% n-decane,.0% 0.mL -U Isoparaffins Mix isoparaffins (typical values shown). Isopentane,.%,-Dimethylbutane,.% -Methylpentane,.% -Methylpentane,.%,-Dimethylpentane,.%,-Dimethylpentane,.%,,-Trimethylbutane,.%,-Dimethylpentane,.0% -Methylhexane,.%,-Dimethylpentane,.% -Methylhexane,.% -Ethylpentane,.00%,-Dimethylhexane,.%,-Dimethylhexane,.%,,-Trimethylpentane,.%,-Dimethylhexane,.%,-Dimethylhexane,.% -Methylheptane,.% -Methylheptane,.% -Methylheptane,.% -Ethylhexane,.%,-Dimethylheptane,.0%,-Dimethylheptane (D), 0.%,-Dimethylheptane,.%,-Dimethylheptane (L), 0.%,-Dimethylheptane,.0%,-Dimethylheptane (D),.%,-Dimethylheptane (L),.0% -Methyloctane,.0% -Methyloctane,.%,-Diethylpentane,.%,-Dimethyloctane,.%,-Dimethyloctane,.%,-Dimethyloctane,.% -Methylnonane,.% -Ethyloctane,.0% -Methylnonane,.% 0.mL -U Naphthenes Mix 0 naphthenes (typical values shown). Cyclopentane,.0% Methylcyclopentane,.% Cyclohexane,.%,-Dimethylcyclopentane,.0% cis-,-dimethylcyclopentane,.% trans-,-dimethylcyclopentane,.% trans-,-dimethylcyclopentane,.0% Methylcyclohexane,.% Ethylcyclopentane,.% ctc-,,-trimethylcyclopentane,.% ctc-,,-trimethylcyclopentane,.% cct-,,-trimethylcyclopentane,.% trans-,-dimethylcyclohexane,.% -Ethyl--methylcyclopentane,.% trans-,-dimethylcyclohexane,.% ccc-,,-trimethylcyclopentane,.% Isopropylcyclopentane,.% cis-,-dimethylcyclohexane,.0% n-propylcyclopentane,.% ccc-,,-trimethylcyclohexane,.%,,-trimethylcyclohexane,.% ctt-,,-trimethylcyclohexane,.0% ctc-,,-trimethylcyclohexane,.0%,,-trimethylcyclohexane,.% Isobutylcyclopentane,.% Isopropylcyclohexane,.% n-butylcyclopentane,.0% Isobutylcyclohexane,.0% t--methyl--propylcyclohexane,.% t--methyl--(mp)cyclopentane,.% 0.mL Olefins Mix olefins (typical values shown). -Methyl--butene,.% trans--heptene,.% -Pentene,.0% cis--heptene,.% -Methyl--butene,.0% trans--heptene,.0% -Methyl-,-butadiene,.% cis--heptene,.% trans--pentene,.0% -Octene,.0% cis--pentene,.0% trans--octene,.0% -Methylpentene- cis--octene,.% -Hexene,.% -Nonene,.% trans--hexene,.% trans--nonene,.% -Methylpentene-,.0% cis--nonene,.% cis--hexene,.% trans--nonene,.0% -Heptene,.% cis--nonene,.% -Decene,.% 0.mL Aromatics Mix aromatics (typical values shown). Benzene,.% Toluene,.% Ethylbenzene,.0% m-xylene,.% p-xylene,. % o-xylene,.0% Isopropylbenzene,.% n-propylbenzene,.0% -Methyl--ethylbenzene,.0% -Methyl--ethylbenzene,.%,,-Trimethylbenzene,.% -Methyl--ethylbenzene,.0%,,-Trimethylbenzene,.% tert-butylbenzene,.% Isobutylbenzene, 0.% sec-butylbenzene,.% -Methyl--isopropylbenzene,.0% -Methyl--isopropylbenzene,.% -Methyl--isopropylbenzene,.% -Methyl--n-propylbenzene,.% -Methyl--n-propylbenzene,.% n-butylbenzene,.%,-dimethyl--ethylbenzene,.0%,-diethylbenzene,.0% -Methyl--n-propylbenzene,.%,-Dimethyl--ethylbenzene,.%,-Dimethyl--ethylbenzene,.0%,-Dimethyl--ethylbenzene,.0%,-Dimethyl--ethylbenzene,.%,,,-Tetramethylbenzene,.% -Methylbutylbenzene,.% tert--butyl--methylbenzene,.% n-pentylbenzene,.% t--butyl,,-dimethylbenzene,.0% t--butyl--ethylbenzene,.00%,,-triethylbenzene,.0%,,-triethylbenzene,.0% n-hexylbenzene,.% 0.mL Bulletin

34 P-I-A-N-O Mix A quantitative mix of the n-paraffins, isoparaffins, aromatics, naphthenes, and olefins in the five mixes described on the previous page. Data sheets for each class of compounds list weight percent, mole percent, and other information for each component. Approximate weight percentages: n-paraffins.%, isoparaffins.%, aromatics.%, naphthenes 0.%, olefins.%. 0.mL -U P-I-A-N-O Kit 0.mL of P-I-A-N-O Mix, plus 0.mL each of the n-paraffins, isoparaffins, aromatics, naphthenes, and olefins mixes. Complete information included. Kit -U Included with P-I-A-N-O Mix and P-I-A-N-O Kit, Data Disc Makes it Easier to Organize P-I-A-N-O Data The P-I-A-N-O CertaDisc Certification Worksheet and Database can greatly simplify the task of organizing certification data. A worksheet (Lotus -- format) contains complete certification data for the n-paraffins, isoparaffins, aromatics, naphthenes, and olefins mixes, and for the P-I-A-N-O mixture. Calculations are easily performed with a few keystrokes. dbase III PLUS programs, designed for use with the Lotus worksheet, include information on all compounds. The programs enable you to perform searches based on retention times, making it easier to identify peaks. ½",. MEG disc. The disc is identified by lot number. (Note: the lot number of data is the vendor lot number and does not match the Supelco lot number of the same product.) We can custom-manufacture petroleum standards. Please inquire. ASTM D Detailed Analysis of Petroleum Naphthas Through n-nonane ASTM D Qualitative Column Evaluation Mix Use this mix of hydrocarbons for assessing column performance. Nominal 0.-.0% by weight in -methylpentane. Toluene, 0.% -Methylheptane, % n-heptane, % -Methylheptane, %,,-Trimethylpentane, % n-octane, % ml 00 ASTM D Splitter Linearity Check Mix Use this quantitative mix of hydrocarbons to determine proper system performance and detector response factors. Neat at 0% by weight, each component. n-hexane Toluene n-heptane -Methylhexane n-octane,-dimethylhexane n-nonane -Methylheptane Benzene,-Dimethylheptane 00mg 0,,-Trimethylpentane Neat, 00mg. 00mg 00 Highly Characterized Reference Materials The following standards, taken from refinery process streams and exhaustively analyzed by GC/FID and GC/MS, are recommended for evaluating process performance, identifying sources of contamination, PIANO analysis, method development, and training. Each comes with a comprehensive data packet containing quantitative and qualitative data and chromatograms using a 00-meter Petrocol DH column. Petroleum Refinery Reformate ml Petroleum Refinery Pyrolysis Gasoline (Py Gas) ml 0-U Petroleum Refinery Heavy Straight Run Naphtha ml Qualitative Reference Materials These refinery reference materials are the actual materials used in the ASTM D round-robin method validation stage. They are referenced in the method, and are used for establishing component retention times for identification purposes. Each sample is accompanied by a comprehensive data booklet containing an expanded detailed chromatogram using a Petrocol DH 0. column, with identified peaks. ASTM D Qualitative Reference Alkylate Standard Actual refinery alkylation product; approximately 0 identified components. Neat fraction. x ml -U ASTM D Qualitative Reference Reformate Standard Actual refinery reformer product; approximately 0 identified components. Neat fraction. x ml ASTM D Qualitative Reference Naphtha Standard Actual refinery naphtha product; approximately 00 identified components. Neat fraction. x ml -U ASTM D Qualitative Reference Refinery Standards Kit x ml of each of the three qualitative reference standards (alkylate, naphtha, and reformate). Kit Refinery Wastes Skinner List Skinner Acid Extractables Mix 00µg/mL each component in methylene chloride. Benzenethiol -Methylphenol,-Dimethylphenol -Methylphenol,-Dinitrophenol -Nitrophenol -Methylphenol Phenol ml -U Bulletin

35 ASTM D Analysis of Petroleum Waxes by GC Qualitative and quantitative mixes of n- paraffins used for determining column resolution, retention times, response factors, and internal standard preparation. ASTM D Column Resolution Test Mix Use to determine column resolution. 0.0 weight percent each of nc0 and nc in cyclohexane. ml 00 x ml 0 Retention Time Standards Neat qualitative weight percent mixes of n- paraffins used to establish retention times from C to C or from C to C0 (components listed at right). ASTM D C-C Retention Time Mix 00mg 0 ASTM D C-C0 Retention Time Mix 00mg 00 Quantitative Linearity Standards Accurately prepared weight percent mixes of n-paraffins used to determine response factors from C to C or from C to C0 (see table of components listed below). ASTM D C-C Linearity Standard ml 0 x ml 0 ASTM D C-C0 Linearity Standard ml 0 x ml Com- Wt. % Wt. % Wt. % Wt. % ponent Neat Solution Neat Solution C. 0.0 C. 0.0 C C C C C C C C C C C C C C Internal Standards n-hexadecane (nc), neat or in solution, added to wax samples as the internal standard. ASTM D n-hexadecane Internal Standard ml 0 ASTM D n-hexadecane Internal Standard Stock Solution In cyclohexane. 0. weight percent. 0mL 00 Qualitative n-paraffin Mixtures For determining retention indices and retention times. Neat mixes contain equal concentrations of the n-paraffins. Solutions contain % (wt./wt.) of each component in n-octane. C, C, C, C 00mg 00 C, C, C, C0 00mg 0 C0, C, C, C 00mg 0 C, C0, C, C ml 0 C, C, C, C ml 0 C, C, C, C ml 0 High Molecular Weight Hydrocarbon Standards For high temperature SIMDIS or GC analyses. Polywaxes are polyethylene waxes having average molecular weights of 00 and Dalton, respectively. Ethylene oligomers range in carbon number from approximately C0 to C00+ and are useful for establishing retention times. n-tetratetracontane (nc) Neat, 0mg Pentacontane (nc0) Neat, 0mg Hexacontane (nc0) Neat, 0mg Polywax 00 Neat, 000mg 0mg/mL in p-xylene, x ml 0-U Polywax Neat, 000mg 0mg/mL in p-xylene, x ml Bulletin

36 ASTM D0 Boiling Range Distribution of Gasoline (00 F maximum) These qualitative and quantitative hydrocarbon blends are prepared according to ASTM recommendations. Calibration mixes are either volume/volume or weight/weight formulations as indicated. Nominal concentration of actual values will differ from lot to lot. Qualitative calibration references are prepared to approximate weight/weight (±0%) specifications and, because of the presence of gases, are not intended for quantitative use. All calibration references are packaged in x ml or x ml quantities and are accompanied by a data sheet. ASTM D0 Qualitative Calibration Mix This mixture consists of the following components in the approximate proportions (w/w) indicated: n-propane,.% n-octane,.% -Methylpropane,.% p-xylene,.0% n-butane, % n-propylbenzene,.% -Methylbutane,.% n-decane,.% n-pentane,.% n-butylbenzene,.% -Methylpentane,.% n-dodecane,.% n-hexane,.% n-tridecane,.%,-dimethylpentane,.% n-tetradecane,.% n-heptane,.% n-pentadecane,.% Toluene, 0.% x ml 0 x ml ASTM D0 Quantitative Calibration Mix This mixture consists of the following components in the proportions (v/v) indicated: -Methylbutane, 0.% p-xylene,.0% n-pentane,.% n-propylbenzene,.% -Methylpentane,.% n-decane,.% n-hexane,.% n-butylbenzene,.%,-dimethylpentane,.% n-dodecane,.% n-heptane, 0.% n-tridecane,.% Toluene,.% n-tetradecane,.% n-octane,.% n-pentadecane,.% x ml 0 x ml ASTM D/D0 Column Resolution Test Mix This mixture consists of the following components at % (w/v) in n-octane: n-hexadecane n-octadecane x ml 0 x ml ASTM D Quantitative Calibration Mix Use this mixture to calibrate retention times for boiling point correlation and to determine detector response factors and overall system performance. n-paraffins in the approximate proportions (w/w) indicated: n-pentane, % n-hexadecane, 0% n-hexane, % n-octadecane, % n-heptane, % n-eicosane, % n-octane, % n-tetracosane, % n-nonane, % n-octacosane, % n-decane, % n-dotriacontane, % n-undecane, % n-hexatriacontane, % n-dodecane, % n-tetracontane, % n-tetradecane, 0% n-tetratetracontane, % x ml 00 x ml 00 ASTM D Quantitative Calibration Solution This quantitative mixture is prepared in carbon disulfide at 0. weight percent each component (except for nc and nc, which are at weight percent). This mix can be used for assessing column resolution as well as for quantitative analyses. n-pentane n-hexadecane n-hexane n-heptadecane n-heptane n-octadecane n-octane n-eicosane n-nonane n-tetracosane n-decane n-octacosane n-undecane n-dotriacontane n-dodecane n-hexatriacontane n-tetradecane n-tetracontane n-pentadecane n-tetratetracontane ml 00 x ml 00 ASTM D Reference Gas Oil Sample, Lot This sample is a petroleum fraction having a boiling range from 0 F to 0 F, evaluated in round-robin studies by the ASTM. Use this sample and the supplied ASTM boiling range consensus values to evaluate system performance. x ml 0 x ml ASTM D0 Boiling Range Distribution of Crude Petroleum ASTM D0 Crude Oil Quantitative Standard Use this mixture to determine detector response factors (slight warming of this mixture may be necessary to dissolve all components completely). Equal weights of the following hydrocarbons: n-decane n-octadecane n-undecane n-eicosane n-dodecane n-tetracosane n-tridecane n-octacosane n-tetradecane n-dotriacontane n-pentadecane n-hexatriacontane n-hexadecane n-tetracontane n-heptadecane n-tetratetracontane ml ASTM D0 Crude Oil Qualitative Standard Combine this mixture with to prepare a calibration mixture covering C to C for calibrating retention times for boiling point correlation. Approximate weight percent concentrations. n-propane, 0% n-heptane, % n-butane, % n-octane, % n-pentane, % n-nonane, % n-hexane, % ml ASTM D0 Crude Oil Internal Standard Equal weights of the listed hydrocarbons. Add this mixture of n-paraffins to the crude oil sample as the internal standard. n-tetradecane n-hexadecane n-pentadecane n-heptadecane ml Bulletin

37 ASTM D Determination of Oxygenates (Ethers and Alcohols) in Gasoline by GC The following calibration standards are designed for use with ASTM D for determining oxygenates in gasoline. Along with valve timing and peak identification mixes, two sets of quantitative calibration mixes are available, both with and without the method-specified internal standard. The internal standard-free mixes are packaged in quantities of approximately.ml to allow the addition of the internal standard. Blends already containing the internal standard are packaged in ml quantities. All components used in the preparation of these standards have been analyzed for purity, water content, and the presence of other method components to 0.0%. Blends have been prepared using precise gravimetric techniques exceeding the requirements of ASTM Method D. Corrections are made for common impurities. All calibration blends are provided with a chromatogram and data verifying the purity and identity of the raw material. Nominal Weight % ic:xylene Ethanol t-butanol MTBE t-pentanol (:) DME D Quantitative Calibration Mixes (Without Internal Standard),.mL in isooctane:xylene (:). Mix Mix Mix Mix Mix D Quantitative Calibration Mixes (With Internal Standard), ml in isooctane:xylene (:). Mix Mix Mix Mix Mix Internal Standard,-Dimethoxyethane (DME) Neat. ml Calibration Mixes ASTM D Valve Timing Mix Use this mix for determining the proper backflush valve timing. Each of the following components at the indicated quantities. Nominal Wt. % Methylcyclopentane 0 Diisopropyl ether 0 Ethyl tert-butyl ether 0 Methyl tert-butyl ether 0 n-hexane 0 ml Bulletin ASTM D Qualitative Peak ID Mix Use this mix for determining relative retention times of selected oxygenates. Each of the following components at the indicated quantities. Nominal Wt. % Methylcyclopentane.0 Methanol. Ethanol. Isopropanol. tert-butanol. n-propanol. Methyl tert-butyl ether.0 sec-butanol. Diisopropyl ether.0 Isobutanol. Ethyl tert-butyl ether.0 tert-pentanol.,-dimethoxyethane (internal standard).0 n-butanol. Benzene.0 tert-amyl methyl ether. Cat. No ml ASTM D Quantitative Calibration Kit Contains ml of each of the following products. Quantitative Calibration Mix (0) Quantitative Calibration Mix (0) Quantitative Calibration Mix (0) Quantitative Calibration Mix (0) Quantitative Calibration Mix (0) Valve Timing Mix () Qualitative Peak ID Mix () Cat. No Kit ASTM D Calibration Curve Verification Kit Contains each of the following products. Quantitative Calibration Mix (00) Quantitative Calibration Mix (0) Quantitative Calibration Mix (0) Quantitative Calibration Mix (0) Quantitative Calibration Mix (0),-Dimethoxyethane () Cat. No Kit 0 Oxygenate-Free Gasolines Use these low- and high-olefin oxygenatefree gasoline samples to assess the level of hydrocarbon interference under your analysis conditions. Oxygenate-Free Low-Olefin Gasoline Cat. No ml Oxygenate-Free High-Olefin Gasoline Cat. No ml

38 ASTM D Purity of Methyl tert-butyl ether (MTBE) by GC ASTM D MTBE Resolution Test Mix Use this mixture to assess column resolution requirements. Each component at % w/w in MTBE. trans--pentene tert-butanol cis--pentene ml MTBE Quantitative Solutions and Neat Materials For analyses of oxygenates in gasoline. Methyl tert-butyl ether (MTBE) 000µg/mL in methanol. ml tert-amyl methyl ether 000µg/mL in methanol. ml 0 Methyl tert-butyl ether (MTBE) Neat. 000mg 0 tert-amyl methyl ether Neat. 000mg 0 MTBE Contaminant Standards Use the following high and low concentration mixtures to identify and determine response factors for the common impurities found in MTBE. tert-amyl methyl ether Pentane tert-butanol cis--pentene tert-butyl ethyl ether trans--pentene Methanol Triisobutylene -Methylbutane (mixed isomers) -Methyl--butene,,-Trimethyl-- pentene ASTM D MTBE Contaminants (High) Mix A Each component at % w/w in MTBE. ASTM D MTBE Contaminants (Low) Mix B Each component at 0.% w/w in MTBE. NIST-Traceable Calibration Standards for Oxygenated Fuels Oxyblend standards, developed by Scott Specialty Gases Inc., are calibration reference materials that help you accurately determine the oxygen content of fuels in compliance with the Clean Air Act of 0. Both standards offered by Supelco are dual-certified, have interlocking concentration values, and are traceable to the National Institute of Standards and Technology (NIST) using NIST traceable reference materials (NTRMs). Oxyblend standards are dual certified because they undergo two rigorous independent methods of certification one during the gravimetric blending step, the other using proprietary laboratory analytical procedures. Scott further ensures the accuracy of the standard by employing an exclusive interlocking feature: the concentrations determined from both certification processes must agree within ±%. Besides being traceable to NIST by weight through the gravimetric blending step, Oxyblend standards are available with two levels of analytical traceability. Oxyblend NTRM standards represent the highest level of traceability available. Scott is the first company to provide NTRMs for oxygenate analysis. Oxyblend standards are traceable to NIST because an NTRM is used to calibrate the instrumentation. Oxyblend standards are packaged in 0mL and 0mL quantities and are available in eight standard mixtures with RFA gasoline background. There are six ampuls per box four traceable reference materials and two instrumentation blanks. Scott Oxyblend NTRM Standards, 0mL These products are directly traced to NIST SRMs. Weight % in RFA NTRM methyl tert-butyl ether.0. NTRM ethanol 0.. NTRM tert-amyl methyl ether.. NTRM ethyl tert-butyl ether.. 00-U Scott Oxyblend NIST-Traceable Standards, 0mL These products are traced to NTRMs by Scott Specialty Gases, Inc. Weight % in RFA Oxyblend methyl tert-butyl ether.. Oxyblend ethanol 0. Oxyblend methyl tert-butyl ether &. tert-amyl methyl ether. 0-U Oxyblend tert-amyl methyl ether.. Oxyblend ethyl tert-butyl ether.. Additional Oxyblend mixtures may be available. Please contact our Technical Service department for details. Bulletin

39 ASTM D0 Aromatics in Gasoline The following calibration standards were developed for determining aromatics in gasoline according to ASTM 0. Standards include calibration blends both with and without internal standard. The internal standard-free blends are packaged in quantities of approximately ml to facilitate reference standard preparation. All calibration blends are provided with a chromatogram and data verifying the purity and identity of the raw material. All raw materials used are fully characterized, as described for ASTM D methods. Nominal Weight (%) Ethyl-,,-Trimethyl- Benzene Toluene benzene o-xylene benzene Isooctane -Hexanone D0 Quantitative Calibration Mixes (Without Internal Standard), ml in isooctane. Mix U Mix Mix Mix U Mix U D0 Quantitative Calibration Mixes (With Internal Standard), ml in isooctane. Mix U Mix U Mix U Mix U Mix U Internal Standard -Hexanone Neat ml -U Calibration Mixes D0 Selectivity Check Standard Each of the following components at the indicated quantities. Nominal Wt. % n-dodecane. Isooctane. ml -U D0 Valve Timing Calibration Mix Each of the following components at the indicated quantities. Nominal Wt. % Benzene. Toluene. Ethylbenzene.0 o-xylene.0 -Hexanone (internal standard) 0.0 Isooctane.0 ml -U WSPA RM- Gasoline Reference Material This multipurpose reference material was developed by Scott Specialty Gases Inc. under the sponsorship of the Western States Petroleum Association (WSPA), based on specifications for California Phase II reformulated gasoline (RFG). Consensus values were established by labs, including the California Air Resources Board (CARB) and are assured by WSPA. The specifications are shown below. ASTM Method Constituents Concentration Units D Oxygenate (Methyl tert-butyl ether).0% WO D0 Total aromatics.% w/w D0 Benzene 0.% w/w D Olefins.% LV D/D Sulfur 0.ppm w/w D Reid vapor pressure. psi x 0mL 0 D0 Quantitative Calibration Kit Contains ml of each of the following products. Quantitative Calibration Mix (0-U) Quantitative Calibration Mix (-U) Quantitative Calibration Mix (-U) Quantitative Calibration Mix (-U) Quantitative Calibration Mix (-U) Valve Timing Calibration Mix (-U) Selectivity Check Standard (-U) Kit ASTM D Aromatics in Gasoline by GC/MS ASTM D/EPA Aromatics Internal Standard Mix Each of the following components at the weight percent indicated. Nominal Wt. % Benzene-d 0 Ethylbenzene-d 0 0 Naphthalene-d 0 x 0.mL x.0ml Weight percent oxygen. Liquid volume. Bulletin

40 ASTM D Oxygenates in Gasoline by GC and OFID Detection The US EPA is requiring the characterization of fuels by capillary oxygen flame ionization detector (OFID) for oxygenate levels in gasoline. ASTM D was developed to meet these requirements. The following standards were prepared according to the specifications of the method. All raw materials used are characterized fully as described for the ASTM D standards. Calibration standards are available both with and without internal standard. The internal standard-free standards are packaged in quantities of approximately.ml to allow for the addition of the internal standard. Calibration standards containing the internal standard are available in ml quantities. Calibration check solutions also are available. Nominal Weight % Synthetic Methanol Ethanol t-butanol MTBE Gasoline DME EPA OFID Quantitative Calibration Mixes (Without Internal Standard),.mL Mix Mix Mix Mix Mix EPA OFID Quantitative Calibration Mixes (With Internal Standard), ml* Mix Mix Mix Mix Mix * The nominal concentrations are calculated by taking into account the mass of internal standard added. In addition to the EPA-required set shown above, similar calibration blends are available with other alcohols and ethers. We can also prepare blends with different internal standards. Please inquire. EPA/ASTM D OFID Synthetic Gasoline Blank These products meet all requirements of a gasoline, including simulated distillation, flash point, and octane number. Supelco synthetic fuel is free of the interfering contaminants, olefins, and oxygenates sometimes associated with synthetic fuels. ml.ml EPA/ASTM D OFID Quantitative Calibration Check Standard (Without Internal Standard) Each of the following components at the indicated quantities. Nominal Wt. % Methanol.0 Ethanol.0 tert-butanol.0 Methyl tert-butyl ether.0 Synthetic gasoline.0.ml We can custom-manufacture petroleum standards. Please inquire. EPA/ASTM D OFID Quantitative Calibration Check Standard (With Internal Standard) Each of the following components at the indicated quantities. Nominal Wt. % Methanol.0 Ethanol.0 tert-butanol. Methyl tert-butyl ether.0 Synthetic gasoline. DME (internal standard).00 ml EPA/ASTM D OFID Quantitative Calibration Check Standard (With Internal Standard) Each of the following components at the indicated quantities. Nominal Wt. % Methanol.0 Ethanol.0 tert-butanol. Methyl tert-butyl ether.0 Synthetic gasoline. -Butanol (internal standard).00 ml EPA/ASTM D OFID Spiking Solutions Each of the following components at the indicated quantities. Nominal Wt. % Methanol. Ethanol. tert-butanol. Methyl tert-butyl ether. ml 0.mL EPA/ASTM D OFID Quantitative Calibration Kit Contains each of the following products. Quantitative Calibration Mix (0) Quantitative Calibration Mix () Quantitative Calibration Mix () Quantitative Calibration Mix () Quantitative Calibration Mix () Synthetic Gasoline Blank () DME internal standard () Kit EPA/ASTM D OFID Quantitative Calibration Kit Contains each of the following products. Quantitative Calibration Mix () Quantitative Calibration Mix () Quantitative Calibration Mix () Quantitative Calibration Mix () Quantitative Calibration Mix () Synthetic Gasoline Blank () Kit 0 Bulletin

41 Hydrocarbon and Chemical Kits PolyScience and Supelco chemical kits contain neat components, solutions, or mixes as indicated. A vendor certificate of analysis documents compound purity and expiration date. Amines Kits Amines Kit C Primary amines, ml each, individually packaged. n-butylamine Cyclohexylamine Isobutylamine Benzylamine sec-butylamine n-heptylamine tert-butylamine n-octylamine n-amylamine n-decylamine iso-amylamine n-dodecylamine,.g n-hexylamine n-tetradecylamine,.g Plus mixture (by weight), ampul: n-propylamine,.% n-hexylamine,.% n-butylamine,.0% Kit Amines Kit C Secondary and tertiary amines, ml each, individually packaged. Diethylamine Dicyclohexylamine Di-n-propylamine Didodecylamine,.g Di-isopropylamine Triethylamine Di-n-butylamine Tri-n-propylamine Di-isobutylamine Tri-n-butylamine Di-n-hexylamine Tri-n-hexylamine Dibenzylamine Tribenzylamine Plus mixture (by weight), ampul: Di-n-butylamine,.0% Cyclohexylamine,.% Di-n-propylamine, 0.% Diethylamine,.0% Kit -U Sulfur Kit Mercaptans Kit 0C ml each, individually packaged. n-propyl mercaptan tert-amyl mercaptan Isopropyl mercaptan n-hexyl mercaptan n-butyl mercaptan n-heptyl mercaptan sec-butyl mercaptan Diethyl sulfide tert-butyl mercaptan Diallyl sulfide iso-butyl mercaptan Di-n-propyl sulfide n-amyl mercaptan Di-n-butyl sulfide Plus mixture (by weight), ampul: Ethyl mercaptan,.% n-propyl mercaptan,.% iso-butyl mercaptan,.% n-butyl mercaptan,.% Kit 0 Alcohols Kits Alcohols Kit C Alcohols, C to C, ml each, individually packaged in serum bottles. Methanol -Butanol Ethanol -Methyl--butanol -Propanol -Methyl--butanol -Propanol -Methyl--butanol -Methyl--propanol -Pentanol -Methyl--propanol -Pentanol -Butanol -Pentanol Plus mixture (by weight), one ampul: -Propanol (.0%) -Methyl--pentanol -Methyl--propanol (.0%) (.%) -Butanol (.%) -Ethyl--butanol -Pentanol (.0%) (.%) Kit 0-U Bulletin Alcohols Kit C Alcohols, normal C to C, ml each, individually packaged in serum bottles. -Hexanol -Tridecanol -Heptanol -Tetradecanol -Octanol -Pentadecanol -Nonanol -Hexadecanol -Decanol -Octadecanol -Undecanol -Eicosanol -Dodecanol -Docosanol Plus mixture (by weight), ampul: -Butanol (.%) -Hexanol (.%) -Pentanol (.%) -Heptanol (.%) 0% solution in ethylbenzene. % solution in ethylbenzene. Kit Alcohols Kit C Alcohols, C-C, ml each, individually packaged in serum bottles. -Hexanol -Ethyl--butanol -Hexanol,-Dimethyl--butanol -Hexanol -Heptanol -Methyl--pentanol -Heptanol -Methyl--pentanol -Octanol -Methyl--pentanol -Octanol -Methyl--pentanol,-Dimethyl--pentanol Plus mixture (by weight), ampul: -Methyl--pentanol (.0%) -Hexanol (.%) -Pentanol (.%) Cyclohexanol (.%) -Ethyl--butanol (.0%) -Heptanol (.%) Kit Hydrocarbons Kits Branched Alkanes Kit D Alkanes, C-C, ml each, individually packaged in serum bottles. -Methylpentane -Methylheptane -Methylpentane,-Dimethylhexane,-Dimethylbutane,,-Trimethylpentane,-Dimethylbutane,,-Trimethylpentane -Methylhexane,,-Trimethylhexane,-Dimethylpentane Cyclohexane,-Dimethylpentane Methylcyclohexane Plus mixture (by weight), ampul: -Methylpentane (%),,-Trimethylpentane (%) n-hexane (%),-Dimethylhexane (%),-Dimethylpentane (%) Kit -U Hydrocarbons Kit C Alkenes, C-C0, ml each, individually packaged in serum bottles. -Hexene (cis, trans)-heptene (cis, trans)-hexene -Heptene -Methyl--pentene -Octene -Methyl--pentene -Octene -Methyl- -Ethyl--hexene (cis, trans)-pentene -Nonene -Ethyl--butene -Decene -Heptene Plus mixture (by weight), ampul: -Pentene (.%) -Octene (.%) -Hexene (.%) -Nonene (0.%) -Heptene (.%) Kit Alkenes Kit D Alkenes, C-C, ml each, individually packaged in vials and/or serum bottles.,,-trimethyl--pentene -Dodecene,,-Trimethyl--pentene -Tridecene -Nonene -Tetradecene (cis, trans)-nonene -Hexadecene,,-Trimethyl--hexene -Octadecene -Decene -Eicosene -Undecene -Docosene Plus mixture (by weight), ampul: -Decene (0%) Octadecene (0%) -Dodecene (%) Hexadecene (%) -Tetradecene (0%) Kit Benzenoid Hydrocarbons Kit ml each, individually packaged in ampuls. Benzene Mesitylene Toluene p-cymene o-xylene n-propylbenzene m-xylene n-butylbenzene p-xylene n-hexylbenzene Ethylbenzene n-octylbenzene Cumene n-decylbenzene Plus mixture (by weight), ampul: Benzene (0%) Toluene (0%) m-xylene (0%) o-xylene (0%) p-cymene (0%) Kit Aliphatic HC Kit Individually packaged in ampuls and vials. n-pentane,.0g n-tetracontane, 0.g n-hexane,.0g n-tetratetracontane, 0.g n-heptane,.0g -Octene, 0.g n-octane,.0g -Decene, 0.g n-nonane,.0g -Dodecene, 0.g n-decane,.0g -Tetradecene, 0.g n-dodecane,.0g -Hexadecene, 0.g n-tetradecane,.0g -Octadecene, 0.g n-hexadecane, 0.g -Eicosene, 0.g n-octadecane,.0g Isooctane,.0g n-eicosane,.0g Squalane,.0g n-heneicosane, 0.g Squalene,.0g n-docosane, 0.g n-paraffin mixtures, 0.g: n-tricosane, 0.g -00 C, C, C, C n-tetracosane,.0g -0 C, C, C, C0 n-pentacosane, 0.g -0 C0, C, C, C n-hexacosane, 0.g n-paraffin mixtures, 0.g: n-octacosane,.0g -0 C, C0, C, C n-triacontane, 0.g -0 C, C, C, C n-dotriacontane, 0.g -0 C, C, C, C n-tetratriacontane, 0.g n-hexatriacontane, 0.g In ml of chloroform. Kit

42 Semivolatile Compounds Aldehydes Kit 0E ml each, individually packaged. Purity at least %. individual compounds. Propionaldehyde Nonanal Butyraldehyde Decanal Isobutyraldehyde Hendecanal Valeraldehyde Dodecanal Isovaleraldehyde Tridecanal Hexanal Tetradecanal Heptaldehyde Benzaldehyde Octanal Kit 0% solution in toluene Basic Internal Standards Kit 00mg each, unless noted otherwise, individually packaged. Purity %, unless noted otherwise. individual compounds, plus in vials. Anthracene Methyl heptadecanoate, mg,-bifluorene Naphthalene, mg Biphenyl, 0mg Octahydroanthracene, 0mg -a-cholestane, mg n-phenylcarbazole Dibenzothiophene -Phenylindole,-Diphenylethane trans Stilbene (%) n-docosane Tetraphenylethylene n-ethylcarbazole, 0mg Tetratriacontane Fluoranthene,,-Triphenylbenzene Heptadecanoic acid, mg Triphenylethylene n-hexadecane Triphenylmethane n-hexacosane Xanthene plus: 0.mL, ml, ml micro reaction vessels (one each),,,,,,,-octahydroanthracene, 0mg Kit Normal Ketones Kit C Normal ketones, ml each, individually packaged. individual compounds, plus in mix. Purity %. -Butanone -Octanone -Pentanone -Octanone -Pentanone -Nonanone -Hexanone -Nonanone -Heptanone -Methylcyclohexanone -Heptanone -Methylcyclohexanone -Heptanone -Methylcyclohexanone plus mixture: -Pentanone,.% (by weight) -Methyl--pentanone,.% -Heptanone,.% -Heptanone,.% ml 0 Branched Ketones Kit D Branched ketones, ml each, individually packaged. Purity %. individual compounds, plus in mix. -Methyl--butanone -Methyl--heptanone,-Dimethyl--butanone -Methyl--heptanone -Methyl--pentanone,-Dimethyl-- -Methyl--pentanone heptanone,-dimethyl--pentanone -Methyl--penten--one -Methyl--hexanone -Methyl--hexanone Acetophenone Cyclopentanone Cyclohexanone plus mixture (by weight): -Methyl--hexanone,.% -Heptanone,.% -Methyl--heptanone,.%,-Dimethyl--heptanone,.% Kit 0 Phenols Kit g each, unless noted otherwise, individually packaged. Purity +%, unless noted otherwise. individual compounds. -Bromophenol,-Dinitrophenol -Bromophenol,-Dinitrophenol -Bromophenol -Ethylphenol -Chloro--methylphenol -Ethylphenol -Chloro--methylphenol -Ethylphenol -Chloro--methylphenol, g -Methylphenol -Chlorophenol, g -Methylphenol -Chlorophenol -Methylphenol -Chlorophenol -Nitrophenol,-Dibromophenol -Nitrophenol,-Dibromophenol -Nitrophenol,-Dichlorophenol Pentachlorophenol,-Dichlorophenol, g Phenol,-Dichlorophenol,,-Trichlorophenol,-Dichlorophenol,,-Trichlorophenol,-Dichlorophenol,,-Trichlorophenol,-Dichlorophenol,,-Trichlorophenol,-Dimethylphenol,,-Trichlorophenol, g,-dimethylphenol, 0.g,,-Trichlorophenol,,-Dimethylphenol mg,-dimethylphenol,,-trimethylphenol,-dimethylphenol,,-trimethylphenol,-dimethylphenol,,-trimethylphenol Kit -U Polynuclear Aromatic Hydrocarbons Mix 00mg/mL each component in toluene. 0 components. Anthracene Fluoranthene Benzo(a)anthracene Perylene Benzo(a)pyrene Phenanthrene Benzo(e)pyrene Pyrene Chrysene Triphenylene ml Polystyrene Standards Kits These kits each contain 0mg of each of the six polystyrene standards below, in amber vials. Molecular weight values are established by size exclusion chromatography, and are nominal values. Actual average molecular weight is within + % of this value. Certificate of Analysis included. Low Molecular Weight Standard Kit 0mg of each standard. 00 MW,00 MW 000 MW 0,000 MW 000 MW 0,000 MW Kit High Molecular Weight Standard Kit 0mg of each standard. 0,000 MW 00,000 MW 0,000 MW 00,000 MW 00,000 MW,00,000 MW Kit Bulletin

43 Underground Fuel Spills (UST) BTEX Standards* EPA Gasoline Range UST BTEX Mix S! Organics (GRO) Mix 00µg/mL each component in methanol. Components at indicated concentrations in methanol. components. Benzene m-xylene Ethylbenzene o-xylene Toluene p-xylene ml 0 ml S0 HC BTEX Mix 000µg/mL each component in methanol. Benzene m-xylene Ethylbenzene o-xylene Toluene p-xylene ml HC BTEX/MTBE Mix 000µg/mL each component in methanol. components. Benzene m-xylene Ethylbenzene o-xylene Methyl tert-butyl ether p-xylene Toluene ml 0-U Gasoline Range Organics (GRO) Standards Gasoline Additives Mix 00µg/mL each component in methanol.,-dibromoethane,-dichloroethane Dibromomethane Methyl tert-butyl ether ml 0 Gasoline Range Organics (GRO) Mix 000µg/mL each component in methanol. components. Benzene,,-Trimethylbenzene Ethylbenzene,,-Trimethylbenzene -Methylpentane m-xylene Naphthalene o-xylene Toluene ml -U PVOC Mix 000µg/mL each component in methanol. components. Benzene,,-Trimethylbenzene Ethylbenzene,,-Trimethylbenzene Methyl tert-butyl ether m-xylene Toluene o-xylene ml * BTEX standards also are available in gas. Please inquire. S! Separate Source standards. See our catalog. Bulletin Benzene, 00µg/mL Ethylbenzene, 00µg/mL Heptane, 00µg/mL -Methylpentane, 00µg/mL Toluene, 00µg/mL,,-Trimethylbenzene, 000µg/mL,,-Trimethylpentane, 00µg/mL m-xylene, 000µg/mL o-xylene, 000µg/mL ml -U UST Modified Gasoline Range Organics (GRO) 000µg/mL each component in methanol. 0 components. Benzene,,-Trimethylbenzene Ethylbenzene,,-Trimethylbenzene Methyl tert-butyl ether m-xylene Naphthalene o-xylene Toluene p-xylene ml Revised PVOC/GRO Mix 000µg/mL each component in methanol. 0 components. Benzene,,-Trimethylbenzene Ethylbenzene,,-Trimethylbenzene Methyl tert-butyl ether m-xylene Naphthalene o-xylene Toluene p-xylene ml -U Single Component Standards At concentrations indicated in ml methanol. Surrogate Standards α, α, α -Trifluorotoluene 0,000µg/mL -U -Chlorooctane 0,000µg/mL -U Internal Standard -Chloro--fluorobenzene 000µg/mL Diesel Range Organics (DRO) Standards UST Modified Diesel Range Organics (DRO) 000µg/mL each component in hexane. 0 components. n-decane n-eicosane n-dodecane n-docosane n-tetradecane n-tetracosane n-hexadecane n-hexacosane n-octadecane n-octacosane ml Single Component Standards -Chlorooctadecane (internal std. or surrogate std. for diesel) 0,000µg/mL in methylene chloride ml -U -Fluorobiphenyl (surrogate std. for diesel) 0,000µg/mL in methylene chloride ml -U o-terphenyl (surrogate std. for DRO) 000µg/mL in acetone ml 0,000µg/mL in methylene chloride ml 0-U - -Androstane (internal std. for DRO) 000µg/mL in methylene chloride ml Fuel Standards Single Component Standards Aviation Gasoline n 0,000µg/mL in methanol ml -U Gasoline n 0,000µg/mL in methanol ml -U Jet (Turbine) Fuel n 0,000µg/mL in methanol ml -U JP- Military Fuel Standard 0,000µg/mL in methylene chloride ml -U JP- Military Fuel Standard 0,000µg/mL in methylene chloride ml -U JP- Military Fuel Standard 0,000µg/mL in methylene chloride ml -U Kerosene Reference Standard 0,000µg/mL in hexane ml -U No. Fuel Oil n 0,000µg/mL in methanol ml -U No. Fuel Oil n 0,000µg/mL in methanol ml -U No. Fuel Oil n 0,000µg/mL in hexane ml -U No. Fuel Oil n 0,000µg/mL in hexane ml -U No. Fuel Oil 0,000µg/mL in hexane:chloroform (0:0) ml Refinery grade components.

44 Refinery Wastes Skinner List Calibration Standards Skinner Acid Extractables Mix 00µg/mL each component in methylene chloride. Benzenethiol -Methylphenol,-Dimethylphenol -Methylphenol,-Dinitrophenol -Nitrophenol -Methylphenol Phenol ml -U Stack Gas Effluents VOC Mix 00µg/mL each component in methanol:water (0:0). Acetone Acrylonitrile Benzene Bromodichloromethane Bromoform Bromomethane Carbon disulfide Carbon tetrachloride Chlorobenzene Chlorodibromomethane Chloroethane Chloroform Chloromethane Dibromomethane,-Dichloroethane,-Dichloroethane,-Dichloroethene trans-,-dichloroethene,-dichloropropane cis-,-dichloropropene trans-,-dichloropropene Ethylbenzene Iodomethane Methylene chloride Styrene,,,-Tetrachloroethane Tetrachloroethene Toluene,,-Trichloroethane,,-Trichloroethane Trichloroethene Trichlorofluoromethane,,-Trichloropropane Vinyl chloride m-xylene o-xylene p-xylene ml -U Air Monitoring Standards: ASTM Method description: Analysis of Aldehydes in Air. TO/IP-A Aldehyde/Ketone-DNPH Mix µg/ml each component (aldehyde equivalent) in acetonitrile. components. Acetaldehyde-,-DNPH Formaldehyde-,-DNPH Acrolein-,-DNPH Hexaldehyde-,-DNPH Acetone-,-DNPH Isovaleraldehyde-,-DNPH Benzaldehyde-,-DNPH Propionaldehyde-,-DNPH Butyraldehyde-,-DNPH m-tolualdehyde-,-dnph Crotonaldehyde-,-DNPH o-tolualdehyde-,-dnph,-dimethylbenzaldehyde- p-tolualdehyde-,-dnph,-dnph Valeraldehyde-,-DNPH ml -U ASTM Method description: Analysis of Ethylene Oxide in Air. -Bromoethanol Solution 000µg/mL in toluene. ml Bulletin

45 Supelco Transportable Gases Supelco now offers an expanded line of pure gases and gas mixtures in convenient transportable cylinders, manufactured for Supelco by Scott Specialty Gases. Scott s popular SCOTTY products are available in a variety of sizes and cylinder capacities. The portability of SCOTTY gases makes these products suitable for many types of applications, both in the laboratory and in the field. Eliminate cylinder demurrage or rental Long-term guaranteed stability for extended shelf life Sufficient product in every cylinder for many applications Save on shipping costs Go anywhere portability makes calibration jobs easier Quality accessory equipment available High accuracy increases dependability A large number of calibration gases are available SCOTTY I SCOTTY II SCOTTY IV SCOTTY HP -0 Specifications SCOTTY I Contents: liters Pressure: 0psig Outlet Fitting: Aerosol-type push button with applicator tube Weight: ~00g Dimensions:. x in. D.O.T. Specs: Q SCOTTY II Contents: liters Pressure: 0psig Outlet Fitting: CGA-0-/" NPT F Weight:. lb. Dimensions: x in. D.O.T. Specs: B0 SCOTTY IV Contents: liters Pressure: 00psig Outlet Fitting: CGA- Weight:. lb. Dimensions: x. in. D.O.T. Specs: NRC SCOTTY HP Contents: 0 liters Pressure: 00psig Outlet Fitting: CGA-0 Weight:. lb. Dimensions:. x. in. D.O.T. Specs: AL00 Scott Gases Shelf life year, unless otherwise noted. Gas Composition SCOTTY I SCOTTY II SCOTTY IV SCOTTY HP and Concentration Pure Gases Air, zero (THC < ppm) Argon.% Carbon dioxide.%* Hydrogen.% 0000 Methane.0% Oxygen.% 0000 Two-Component Mixtures Benzene in air (ppm) 00 Benzene in air (0ppm) 00 Benzene in air (00ppm) 00,-Butadiene in nitrogen (0ppm) Carbon dioxide in helium (00ppm)* 000 Carbon dioxide in nitrogen (00ppm) Carbon dioxide in nitrogen (000ppm) Chlorine mixture in nitrogen (0ppm)** 0 Ethylene in air (0ppm)* Ethylene in helium (00ppm) Hydrogen in helium(00ppm) 000 Hydrogen in nitrogen (%) Hydrogen in nitrogen (00ppm) Methane in helium (00ppm) Methane in nitrogen (00ppm) Bulletin