Quantitative Confirmatory Analysis of the NIDA 5 Panel Using Prelude SPLC System and TSQ Quantum Ultra MS

Transcription

1 Quantitativ Confirmatory Analysis of th NIDA Panl Using Prlud SPLC Systm and TSQ Quantum Ultra MS Haiqiang (Bill) Yu, Kristin Van Natta, Marta Kozak Thrmo Fishr Scintific, San Jos, CA, USA

2 Ovrviw Purpos: Dvlop and validat a simpl and fficint quantitativ LC-MS/MS mthod for SAMHSA-compliant confirmatory analysis of panl drug using novl HPLC systm. Mthods: Human urin containing th drugs wr spikd with intrnal standards, nzymatically hydrolyzd, and dilutd. Rsults: Th LC-MS/MS mthod was dvlopd and validatd to comply with SAMHSA guidlins. Introduction Effctiv on Octobr 211, th nw SAMHSA/NIDA guidlins allow implmntation of LC-MS tchniqu to prform NIDA- panl, urin quantitativ confirmatory analysis. LC-MS/MS mthods ar oftn lss complicatd than th prviously implmntd GC-MS/MS mthods bcaus thy do not rquir drivatization. Th NIDA- panl rquirs 6 sparat quantitativ mthods for analysis of THCA, opiats, amphtamins, cocain, phncyclidin and 6-MAM to confirm immunomthod positiv sampls. Hr w dvlopd 6 mthods using a singl sampl prparation procdur, analytical column, mobil phas and instrumnt configuration. Th mthods ar implmntd on nw Thrmo Scintific dual channl Prlud SPLC onlin sampl prparation-liquid chromatography systm, which allows mthod xcution in paralll with a diffrnt mthod on ach channl or th sam mthod on both channls multiplxd to a singl mass spctromtr. Srial MS dtction of multiplxd mthods improvs mass spctromtr utilization tim, incrass laboratory throughput and rducs analysis cost. Th syring pumps and high-prssur, low-volum gradint mixing usd in th Prlud SPLC systm provid nhancd LC prformanc including improvd pak shap and rsolution, stabl rtntion tims and rducd solvnt consumption. Mthods Sampl Prparation Th sampl prp procdur includs glucuronid hydrolysis followd by dilution. For ach sampl a 2-µL aliquot of urin was spikd with 1 µl of intrnal standard solution and 1 µl of β-glucuronidas nzym in ammonium actat buffr, ph=.. Th sampls wr incubatd at 6 C for 2 hours. A 2-µL aliquot of mthanol was addd to ach sampl to stop nzymatic raction. Sampls wr coold down, cntrifugd and dilutd 2-fold with watr, xcpt for THCA, which was dilutd 2-fold with watr. Thn 2 µl of sampl was injctd onto th LC-MS/MS systm. Liquid Chromatography Chromatographic sparations wr prformd with th Prlud SPLC systm by dirct injction onto a Thrmo Scintific Accucor PFP x2.1mm, 2.6 µm analytical column. Th column was maintaind at room tmpratur. Mobil phass A and B consistd of 1 mm ammonium format with.1% formic acid in watr and mthanol, rspctivly. Sparat mthods wr st up to analyz 6- MAM, BE, PCP, and THCA. On mthod was st up for th combination of amphtamin, mthamphtamin, MDA, MDEA and MDMA. A final mthod was usd for th opiats morphin and codin along with hydromorphon, hydrocodon, oxymorphon and oxycodon. Figur 1 shows th LC mthod for analyzing th opiats. Mass Spctromtry MS/MS analysis was carrid out on a Thrmo Scintific Quantum Ultra tripl quadrupol mass spctromtr quippd with a hatd lctrospray ionization (HESI-II) prob. MRM transitions for ach compound ar listd in Tabl 1. Validation Th calibration standards and quality control (QC) sampls wr prpard by spiking compounds into blank urin. Sampls wr procssd as dscribd in th Sampl Prparation sction. Mthods wr validatd in multiplxd mod. Intra- and intr- mthod prcision and accuracy wr dtrmind by analyzing a calibration curv along with rplicat QCs on thr diffrnt days. Matrix ffcts wr dtrmind by comparing pak ara of sampls procssd in multipl lots of urin to that of on procss in watr. Additionally for th opiats, w wr abl to corrlat rsults obtaind with this mthod to thos from a toxicology laboratory validatd mthod. Data Analysis Thrmo Scintific TracFindr softwar was usd for data acquisition and procssing. Data wr procssd with ion ratio confirmation. 2 Quantitativ Confirmatory Analysis of th NIDA Panl Using Prlud SPLC Systm and TSQ Quantum Ultra MS

3 FIGURE 1. LC mthod for sparating morphin and codin. Rsults For ach mthod, prformanc was within SAMHSA/NIDA guidlins. Th quantitation limits (LOQ) for som compounds wr lowr than rquird to dmonstrat mthod capability. Th linar rangs wr 2.-2 ng/ml for PCP and THCA; -2 ng/ml for mthamphtamin, BE and 6-MAM; 1-2 ng/ml for morphin, codin, amphtamin, MDA, and MDMA (Figur 2). Th intra-mthod prcision was <13.%, <3.%, <14.1%, <6.9%, < 9.6%, <1.9% for PCP, BE, 6-MAM, THCA, opiats and amphtamins rspctivly. Th intr-mthod prcision was <8.9%, <3.6%, <1.9%, <8.8%, <7.%, <1.3% for PCP, BE, 6-MAM, THCA, opiats and amphtamins rspctivly. Ths rsults ar summarizd in Tabl 2. Limitd matrix ffcts wr sn and thos wr largly mdiatd by dutratd intrnal standards. Th prcnt rcovry for 8 spikd urin donor sampls was in rang of 8-12% (Tabl 3). Data collctd for opiats with dvlopd mthods corrlatd wll with toxicology laboratory data with cofficint of corrlation >.99 (Figur 4). Implmntation of th dual channl Prlud SPLC systm with syring pumps improvd rtntion tim prcision, chromatographic paks shap and rsolution, thus allowing for short, small solvnt consumption LC mthods whil still kping good data quality. TABLE 1. List of NIDA compounds MRM transitions, cutoff rquirmnts, LOQ and Linar rang Drug MRM (Q: Quantifir) Cutoff (ng/ ml) LOQ (ng/ ml) Linar Rang Amphtamin (Q), Mthamphtamin (Q), MDA (Q), MDMA (Q), MDEA (Q), Bnzoylcgonin (Q), THCA (Q), Phncyclidin (Q), Morphin (Q), Codin (Q), Actylmorphin (Q), Thrmo Scintific Postr Not PN ASMS13_SAMSHA_Prlud_ 6/13S 3

4 FIGURE 2. Rprsntativ calibration curvs for BE, THCA, 6-MAM and PCP. Bnzoylcgonin Y 2 = X ;R^2:.9933; Origin:Ignor;W: 1/X^2;Ara THCA Y = X ;R^2:.9994; Origin:Ignor;W:1/X;Ara Concntration (ng/ml) Concntration (ng/ml) MAM Y = X ;R^2:.9939; Origin:Ignor;W:1/X^2;Ara Phncyclidin Y = X ;R^2:.9916; Origin:Ignor;W:1/X^2;Ara Concntration (ng/ml) Concntration (ng/ml) FIGURE 3. Exampl chromatograms for ach mthod at rspctiv LOQs. Oxymorphon Morphin Hydromorphon Codin Oxycodon Hydrocodon Intnsit y PCP THCA MAM BE Mthamphtami Amphtamin MDA n MDMA MDEA Quantitativ Confirmatory Analysis of th NIDA Panl Using Prlud SPLC Systm and TSQ Quantum Ultra MS

5 TABLE 2. Intra-mthod and Intr-mthod Prcision. Prcision (RSD%) Compound Intra-mthod Intr-mthod LQC MQC HQC LQC MQC HQC Amphtamin MDEA MDA MDMA Mthamphtamin Bnzoylcgonin Phncyclidin 6-Actylmorphin THCA Morphin Codin <1.9 <3.68 < <.33 <3.46 < <6.66 <4.1 < <.2 <4.34 < <.47 <4.2 < <2.21 <2.3 < <6.88 <3.6 < <.87 <3.39 < <7 <2.8 < <8.2 <1.8 < <7.3 <.2 < TABLE 3. Rcovry of 11 drugs in 6 diffrnt urin lots. Urin Lot Amphtamin MDEA MDA MDMA Mthamphtamin Bnzoylcgonin Phncyclidin Actylmorphin THCA Morphin Codin Thrmo Scintific Postr Not PN ASMS13_SAMSHA_Prlud_ 6/13S

6 FIGURE 4. Corrlation of data acquird with Prlud-Ultra mthod compard with data from a toxicology rsarch laboratory validatd mthod. Concntrations from Toxicology Lab (ng/ml) Concntrations from Prlud SPLC (ng/ml) Conclusion An LC-MS/MS mthod for confirmatory analysis of th 11 drugs in th NIDA panl using th Prlud SPLC and TSQ Quantum Ultra MSwas dvlopd and validatd. Th mthod has LOQs that satisfy th SAMSHA cutoff rquirmnts for ths 11 drugs. No matrix intrfrnc wr obsrvd. Th mthod is simpl and fast. Two-channl multiplxing on Prlud SPLC would allow two diffrnt mthods multiplxing in two channls and 3 minuts for a sampl. Acknowldgmnts W would lik to thank Knt Johnson from Pacific Hospital of Long Bach for supplying th comparison sampls. 6 Quantitativ Confirmatory Analysis of th NIDA Panl Using Prlud SPLC Systm and TSQ Quantum Ultra MS

7 213 Thrmo Fishr Scintific Inc. All rights rsrvd. ISO is a tradmark of th Intrnational Standards Organization. All othr tradmarks ar th proprty of Thrmo Fishr Scintific, Inc. and its subsidiaris. Spcifications, trms and pricing ar subjct to chang. Not all products ar availabl in all countris. Plas consult your local sals rprsntativ for dtails. Thrmo Fishr Scintific, San Jos, CA USA is ISO 91:28 Crtifid. Africa-Othr Australia Austria Blgium Canada China Dnmark Europ-Othr Finland/Norway/Swdn Franc Grmany India Italy Japan Latin Amrica Middl East Nthrlands Nw Zaland Russia/CIS South Africa Spain Switzrland UK USA ASMS13_SAMSHA_Prlud_E 6/13S