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1 -.... ** * * *. **. wisam_ali2004@yahoo.com. - - / Oberon 240 SC[Spiromesifen]... /. MRL./... الكلمات المفتاحية: مبيد االوبيرون متبقي المبيدات والخيار. DISSIPATION AND DETERMINATION OF Spiromesifen 240SC RESIDUES IN CUCUMBER AND EFFECT OF FOOD PROCESSING ON RESIDUE Wisam A. Al-Mashhadany, Saleh H. Samir,Abid Alkareem J. Ali Dept.of Plant Protection, College of Agriculture,University of Baghdad, Baghdad,Iraq. National Center for Pesticide Control,State Board for Plant Protection, Ministry of Agriculture,Baghdad,Iraq. ABSTRACT Laboratory and field studies were carried out at College of Agriculture- University of Baghdad and State Board for Plant Protection Ministry of Agriculture to study the dissipation of insecticide, [Spiromesifen] 240SC which used to control insects on Cucumber [plastic houses] by using High Performance Liquid Chromatography [HPLC] technique, besides evaluate the efficacy of some treatments on cucumber fruits [ washing in water and soap, peeling, immersion in saline solution and pickling] to reduce the amount of insecticide residue in ٧٦٩
2 -.... cucumber fruits. Results of Spiromesifen revealed that in first spray the residue in leaves was mg/kg directly after treatment then dropped to 0.09 mg/kg after 28 days. However the residue dissipated faster in second spray. In fruits, the residue in first spray was mg/kg directly after treatment reduced to MRL [Maximum residue limit] in 7 days 0.19 mg/kg, however the dissipation was faster in second spray, the residue reached MRL after 4 days 0.21 mg/kg. Regarding treatment of washing the fruit, Oberon residue was dissipated in first and second spray after 3 days 0.21 mg/kg and 4 days 0.2 mg/kg respectively. Residue of Oberon reduced to 0.04 and 0.06 mg/kg after 21 days respectively. Also, treatment of peeling, saline solution and pickling reduced Spiromesifen residue in first spray to MRL after 2 days [0.21, 0.23 and 0.22] mg/kg respectively, while in second spray, Spiromesifen reduced dropped to MRL after 2 days [0.2, 0.17 and 0.18] mg/kg respectively for previous treatment. Keywords:Oberon,insecticide residue,cucumber. Tetranychus urticae Oberon. [ ] Acetyl CoA-Carboxylase.[ ] Spiromesifen.[] Tow (T. urticae Koch) spotted spider mite %. %-.[] % [] [] Acetamiprid Imidacloprid Buprofezin) (Dinotefuran Aceria guerreronis.[] [... ]. ] [ %.[]. (Spiromesifen) Oberon240SC Tetronic acid.lipid biosynthesis Inhibition Bemisia tabaci Biotype B. argentifolii Trialeurodes vaporariorum ٧٧٠
3 -.... ᵒ Toshka. S&G. //.// (Oberon 240 SC) Spiromesifen :.. / / (-) (-) Spiromesifen [] Deean IPM Eretmocerus mundus Mercet [Hymenoptera : Aphelinidae] Orius (Hemiptera : Anthocoridae) laerigatus Fieber..[] [Maximum Residue Limit] MRL United States Department of MRL (USDA) Agriculture Spiromesifen / 0.2 /. /.. /. /. MRL.[]. /. Spiromesifen / [].[] CO 2 PHI. [] ٧٧١
4 -.... (. -) ( -). :. : (-).[] : - []..[ ] : - [] %.[ ] : - [ ]] - Dal. Decan (-). / / / / / / / / / / -. []. : //. / / / / / / / / / / ٧٧٢
5 % :- HPLC : (SHIMADZU/ LC-20AD)../. ᵒ () (-) /. /.. /. [] /. /. /..(-) :(- ). :. - [] : : - : :..Celite. : : [ ].: : :-.[ ]. - : - % Formic acid./ - - NCN. / / -. : ( : ).. -. Spiromesifen. ٧٧٣
6 -.... (-). :(- ).. -) - MRL ( /. /.. /...( -) / MRL.[3] [] ( -) /. / 0.2 =MRL ) /. (. /. (-) /.. /. MRL. / /. /.[] (-). :(-). ٧٧٤
7 -.... (-). - [ ] %. / 0.23 / /. /. (-). - (-). / HPLC. (٨ (الشكل-.. /. /. [ ]. /. ) HPLC.( % (-). (-). ٧٧٥
8 -.... MRL MRL..[] / 0.2 MRL. [] /.. Penetration [7]. % (-). [ ] - / /. /.. /.(-) /. (- ). (- ). ٧٧٦
9 -.... [10]Pablo, B., J. Contreras, V. Quinto, J. Izquierdo, V. Mansanet and A. Elbert Effects of Oberon 240 SC on bumblebees pollinating greenhouse Tomatoes. Pflanzenschutz-Nachrichten, 3: [11]Babczinski, P. and E.L. Arthur Environmental fate of spiromesifen [Oberon]. Pflanzenschutz-Nachrichten, 3: [12]PMRA Proposed Maximum Residue Limit Spiromesifen. Health Canada Pest Management Regulatory Agency. Departments of the Government of Canada. [13]Weber,E Behaviour of spiromesifen [Oberon]in plants and animals. Pflanzenschutz- Nachrichten58/2005,3: [14]CDPR Spiromesifen. California department of pesticide Regulation. Tracking ID Numbers CRR N thru CRR N, PUBLIC REPORT -2. [15]EPA Analytical method for the determination of residue of CGA and the metabolite CGA in animal and crop substrates by High Performance Liquid Chromotography with detection by UV and Mass Spectrometry including valdation data, Pp235 [16] العزاوي خلود عبد المجيد. ١٩٨٨. تالشي بقايا مبيد الفنتراثايون "سوميثيون" في محصول الخيار في البيوت المحميةإطروحة ماجستير-كلية الزراعة جامعة بغداد ٦٠ ص. [17]Randhawa,M.A.,F.M.Anjum,M.S.Randhawa, A. Ahmed, U. Farooq, M. Abrar and M.A. Randhawa Dissipation of Deltamethrin on supervised vegetable and removal of its residue by household processing. J. Chem. Soc. Pak., 30: [18]Geetanjali, K., S. Santosh and S.N. Naik Food processing a tool to pesticide residue dissipation A review. Food Research International, 42: [19]Nicolaus,B,C.RomijnandB.Lisa Ecot Oxicological profile of the insecticide Oberon.Pflanzenschutz-Nachrichten,3: [20]EFSA Peer Review Report on Spiromesifen. European Food safety Authority Journal, 5[2]: [ 21 ]النوى فؤاد فارس و محمد جمال الحجار ومنذر بدر حلوم. ٢٠٠٤.سلوك الھدم لمبيد الميثوميل في ثمارالخيار تحت الضروف الحقليةوالبيوت البالستيكيةبالساحل السوري. مجلة وقاية النبات العربية ٣٩-٣٥:(١)٢٢. [22]Karanth, N.G.K Challenges of Limiting Pesticide Residues in Fresh Vegetables: The Indian Experience. Food Safety Management in Developing Countries. Proceedings of the International Workshop, India.. Reference: [1]Lois,S.G,H.S.Thomas,N.A.Bruce and B.M. Neela.2001.Pesticide residues in food and cancer risk:a critical analysis.handbook of Pesticide Toxicology,Second Edition[R. Krieger,ed],SanDiego,CA:AcademicPress, [2]Nauen, R., H.J. Schnorbach and A. Elbert The biological profile of spiromesifen (Oberon) A new tetronic acid insecticide / acaricide.pflanzenschutznachrichten,3:417440[ 3]Elbert, A., E. Brück, J. Melgarejo, H. J. Schnorbach and S. Sone Field Development of Oberon for whitefly and mite control in vegetables, cotton, corn, strawberries, ornamentals and tea. Pflanzenschutz- Nachrichten, 3: [4]Marcic, A. D., O. Irena, M. Slavka and P. Pantelija The effects of spiromesifen on life history traits and population growth of two-spotted spider mite (Acari: Tetranychidae). Exp Appl. Acarol., 50: [5]Nauen, R., and S. Konanz Spiromesifen as a new chemical option for resistance management in whiteflies and spider mites. Pflanzenschutz Nachrichten,3: [6]Suganthi A., K. Ramaraju, S. Kuttalam and S. Chandrasekaran Bioefficacy of spiromesifen [Oberon] 240 SC against coconut eriophyid mite Aceria guerreronis Keifer and determination of residues. Journal of Entomology, 3[4]: [7]Schöning,R.2005.An analytical method for the determination of residues of spiromesifen Oberon and its main metabolite in and on plant materials by HPLC with MS/MS-detection. Pflanzenschutz- Nachrich-ten 3: [8]Palumbo,J.C.2009.Spray timing of spiromesifen and buprofezin for managing Bemisia whiteflies in Cantaloupes. Plant Health Progress, [9]DeeAn, J Environmental fate of Spiromesifen. Environmental Monitoring & PestManagement. Department of Pesticide Regulation. Sacramento, CA ٧٧٧
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