Effects of arbuscular mycorrhiza and composted market waste on the performance of Tiannug 1 variety of kenaf (Hibiscus cannabinus Linn.

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1 Available online at Int. J. Biol. Chem. Sci. 8(3): , June 2014 ISSN X (Online), ISSN (Print) Original Paper Effects of arbuscular mycorrhiza and composted market waste on the performance of Tiannug 1 variety of kenaf (Hibiscus cannabinus Linn.) Babatunde SAHEED BADA 1* and Olajire FAGBOLA 2 1 Department of Environmental Management and Toxicology, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria. 2 Department of Agronomy, University of Ibadan, Ibadan, Nigeria. * Corresponding author, bsbada2000@yahoo.com; Tel: ABSTRACT This study determined effects of composted market waste and Arbuscular Mycorrhiza (AM) on Root Colonization (RC) and fibre yield of kenaf. The experimental design was 2 x 12 factorial in a Completely Randomized Design replicated three times. The treatments were with AM (AM + ) and without (AM - ) and twelve levels of soil amendment: 0, NPK 20:10:10 (60 kg N ha -1 ), purely composted market waste (20, 40, 60, 80 and 100 kg N ha -1 ), and composted market waste fortified with superphosphate and urea (20, 40, 60, 80 and 100 kg N ha -1 ). Residual effect of the treatments was also determined. Data on RC and yield were collected and analyzed using descriptive statistics and ANOVA. RC ranged from 14.4 to 78.1%. Inoculated Tiannug 1 at 40 kg N ha -1 composted market waste fortified with superphosphate and urea had significantly (p < 0.05) higher RC (79.8%), bast (9.6 g pot -1 ) and core (19.9 g pot -1 ) yields. For the residual effect, inoculated Tiannug 1 at 100 kg N ha -1 purely composted market waste had significantly (p < 0.05) higher RC (69.0%), bast (2.7 t ha -1 ) and core (5.8 t ha -1 ) yields. Tiannug 1 was highly colonized by AM and the optimum yields observed at 40 kg N ha -1 composted market waste fortified with superphosphate and urea International Formulae Group. All rights reserved. Keywords: Arbuscular mycorrhiza, composted market waste, kenaf, performance. INTRODUCTION Continuous use of inorganic fertilizer results to soil acidification (Bada et al., 2012). Organic manures are excellent sources of organic matter but relatively low in nutrients for example nitrogen, phosphorus and potassium (Bada et al., 2012). Complementary use of organic manure and inorganic fertilizer ensures the availability of nutrient throughout the growth period of crops. The use of composted market waste for crop production is gaining popularity worldwide because it is environmentally friendly, cheaper and easily available compared to inorganic fertilizer. In view of this, complimentary use of low chemical inputs and organic manure may be a cost effective economic strategy (Omueti et al., 2000). As a way of reducing total dependence on the use of fertilizer, an integrated fertility management system focusing on biological approach, which is ecofriendly and less expensive, is desirable. Mycorrhizal symbiosis is well recognized as a biological tool to enhance 2014 International Formulae Group. All rights reserved. DOI :

2 nutrient acquisition in most plants growing on deficient soils (Cardoso and Kuyper, 2006). Mycorrhizal is a symbiotic association between plant root and specialized soil fungi with evidence that it helps plants in nutrient acquisition of immobile nutrients such as P, N, Zn and Cu in deficient soils (Clark and Zeto, 2000; Hodge, 2003; Dare et al., 2008; Ibiremo and Fagbola, 2008). Kenaf (Hibiscus cannabinus L.) stem produces two types of fibre, a coarser fibre in the outer layer (bast) and a finer fibre in the inner (core). Uses of kenaf include fibre and food (Bert, 2002; Zhang, 2003), medicine (Cheng, 2001), medium for mushroom cultivation (Cheng, 2001; Liu, 2003), phytoremediation (Bada and Kalejaiye, 2010; Bada and Raji, 2010; Bada and Umunnakwe, 2011), oil and chemical absorbents (Sameshima, 2000). In addition, the bast fibre can be converted to pulp for newsprint, hydrocarbon free bags, ropes and textiles (Kuchinda and Ogunwole, 2000; Webber et al., 2002). Kenaf fibre is used in making ropes, sack and other domestic purposes such as creating fences and thatching for dwelling (IART, 2010). Kenaf generally gives the greatest response to nitrogen, followed by phosphorus (Wilson, 2003; IART, 2010). Therefore, for sustainable kenaf production in a nutrient degraded soil, there is need to study effect of arbuscular mycorrhizal inoculation and composted market waste application on the growth and yield of kenaf. This will go a long way in reducing total dependence on the use of inorganic fertilizer and also reduce the problem of waste management at little or no cost. The objectives of this study were to investigate the effect of composted market waste application on the root colonization of kenaf by Arbuscular Mycorrhiza (AM); evaluate the effect of AM with composted market waste on growth and yield of kenaf and examine the residual effect of AM inoculation and composted market waste on bast and core yield of kenaf. MATERIALS AND METHODS The screenhouse experiment was carried out at the Institute of Agricultural Research and Training (IART) Moor Plantation, Ibadan on Latitude N and Longitude E in the rainforest of South- Western Nigeria. Description of the planting materials Two types of market waste-based fertilizers (produced by Pacesetter Fertilizer Company, Ibadan, Nigeria) namely: composted market waste fortified with superphosphate and urea (Pacesetter Grade A fertilizer) and purely composted market waste without any additive (Pacesetter Grade B fertilizer), prior to application, were taken to the laboratory for proximate analysis. However, N.P.K. 20:10:10 fertilizer at recommended rate of 60 kg N ha -1 (IART, 2010) was also used. Mycorrhizal inoculum (Glomus mosseae) consisting of chopped roots of the trapping plant, hyphae, spores and soil was collected from the Soil Microbiology Laboratory, Department of Agronomy, University of Ibadan, Ibadan, Nigeria. In an experiment carried out in the same area, Atayese et al. (1993), reported that of all the AM present, Glomus mosseae is the most prominent / abundant. As a result, Glomus mosseae was used in this study. Tiannug 1, one of the highly yielding varieties of kenaf suitable for different agro-ecological zones in Nigeria (IART, 2010) was collected from IART, Ibadan, Nigeria. Experimental design A 2 12 factorial experiment in a Completely Randomized Design with three replicates was carried out to assess the effects of two levels of mycorrhiza (with and without) and twelve levels of fertilizers: 0, NPK 20:10:10 (60 kg N ha -1 ), purely composted market waste (20, 40, 60, 80 and 100 kg N ha -1 ) and composted market waste fortified with superphosphate and urea (20, 40, 60, 80 and 100 kg N ha -1 ) on the root 1152

3 colonization, growth and fibre yield of Tiannug 1. Soil sample collection, preparation, sowing and data collection Representative topsoil (0 15 cm) samples were collected from research farm of IART, Ibadan and thoroughly mixed together. Sub-samples were taken to determine the physical and chemical properties using the methods described in Chopra and Kanwar (1999). Arbuscular mycorrhiza infective propagule was also determined using the Most Probable Number (MPN) technique (Porter 1979). Each pot contained 10 kg soil. Composted market waste fortified with superphosphate and urea (20, 40, 60, 80 and 100 kg N ha -1 ) and purely composted market wastes (20, 40, 60, 80 and 100 kg N ha-1) were thoroughly mixed with the soil in the pots 24 hours before planting. Sixty kg N ha -1 of N.P.K. 20:10:10 fertilizer was applied the third week after planting by side placement. Twenty grams of mycorrhizal inoculum (Glomus mosseae) was applied per pot of those designated as inoculated. The method of application was by filling the pots threequarter way and then evenly spread the mycorrhiza inoculum on it (Carling et al., 1978). The pots were then filled with the rest of the soil and watered. Seeds of Tiannug I were sown at the rate of 4-6 seeds per pot. After germination, the plants were thinned to one per pot and left to grow in the pot for three and half months (14 weeks). Plant height was measured using metre rule and stem diameter (cm) using vernier caliper. After harvesting, bast and core yield were determined using weighing balance. Percentage root colonization was determined using the method described by Giovanneti and Mosse (1980). Assessment of residual effects of AM and fertilizers This was carried out to determine the residual effect of arbuscular mycorrhiza, purely composted market waste, composted market waste fortified with superphosphate and urea, and N:P:K fertilizer on the root colonization, growth and yield of Tiannug 1. Soil preparation and sowing After harvesting, the soil in each pot was thoroughly mixed. Neither fertilizer nor mycorrhiza was applied and kenaf seeds were then sowed. Statistical analyses Data were analyzed using descriptive statistics (mean) and analysis of variance. Standard error was used to separate the means at p < RESULTS Soil characteristics and proximate analysis of fertilizers The soil used in the screenhouse experiment was sandy loam in texture, with a ph of 6.1 (Table 1). Also, the soil organic matter, total nitrogen, available phosphorus and exchangeable potassium were 15.0 g kg -1, 1.5 g kg -1, 2.1 mg kg -1 and 0.19 cmol kg -1 respectively. Arbuscular mycorrhiza infective propagule density was 110 infective propagules per 100 g soil. Composted Market Waste Fortified with Superphosphate and Urea (CMWFWSAU) had higher concentrations of primary nutrient such as nitrogen, phosphorus and potassium than Purely Composted Market Waste (PCMW) (Table 2). Effects of AM and fertilizers on the growth of Tiannug 1 Arbuscular mycorrhiza inoculation and composted market waste application rates significantly affected the stem diameter of Tiannug 1(Table 3). Inoculated Tiannug 1at 0 fertilizer level had higher stem diameter than non-inoculated counterpart at 0 fertilizer level throughout the growth period. Increase in the levels of PCMW and CMWFWSAU without arbuscular mycorrhizal inoculation resulted in the significant (p < 0.001) increase in stem diameter from 20 to 60 kg N ha -1 of both PCMW and CMWFWSAU (Table 3). Without mycorrhizal inoculation, significantly (p < 0.001) higher stem diameter was observed at 60 kg N ha -1 of CMWFWSAU. On the effects of arbuscular mycorrhizal inoculation and fertilizers application, stem diameter of inoculated Tiannug 1 was 1153

4 significantly (p < 0.001) higher than the noninoculated counterpart at 60 kg N ha -1 of NPK, 40 kg N ha -1 of PCMW, 40 and 60 kg N ha -1 of CMWFWSAU from 6 to 14WAP (Table 3). Significantly (p < 0.001) higher stem diameter was observed at 40 kg N ha -1 of CMWFWSAU 14WAP. Mycorrhizal inoculation significantly (p < 0.001) increased plant height at 0 fertilizer level compared to their noninoculated counterpart also at 0 fertilizer level (Table 4). Among the various fertilizer levels without mycorrhizal inoculation, 60 kg N ha -1 of CMWFWSAU had significantly (p < 0.001) higher plant height compared to other levels of composted market waste without mycorrhizal inoculation (Table 4). On the effects of mycorrhizal inoculation and composted market waste application rates, inoculated Tiannug 1 had significantly (p < 0.001) higher plant height than non-inoculated counterpart at 60 kg N ha -1 of NPK, 40 kg N ha -1 of PCMW, 40 and 60 kg N ha -1 of CMWFWSAU from 6 to 14WAP. Significantly (p < 0.001) higher plant height was observed in the inoculated Tiannug 1 at 40 kg N ha -1 of CMWFWSAU. Influence of AM and fertilizers on the colonization and yields of Tiannug 1 Inoculated Tiannug 1 at 0 fertilizer application had significantly (p < 0.001) higher bast yield than their non-inoculated counterpart at 0 fertilizer application (Table 5). Increase in the levels of PCMW and CMWFWSAU without mycorrhizal inoculation significantly (p < 0.001) increased the bast yield from 0 to 60 kg N ha -1 while at 80 kg N ha -1 or more, a decline in bast yield occurred (Table 5). On the other hand, inoculated Tiannug 1 had significantly (p < 0.001) higher bast yield than the noninoculated counterpart at 60 kg N ha -1 of NPK, 40 kg N ha -1 of PCMW, 40 and 60 kg N ha -1 of CMWFWSAU. The highest bast yield was observed at 40 kg N ha -1 of CMWFWSAU (Table 5).Without mycorrhizal inoculation, significantly (p < 0.001) higher core yield was observed at 60 kg N ha -1 of CMWFWSAU (Table 5) Fertilizers application with mycorrhizal inoculation had significantly (p < 0.001) higher percentage mycorrhizal colonization than fertilizers application without mycorrhizal inoculation at 40 kg N ha -1 of PCMW and CMWFWSAU. Residual effects of AM and fertilizers on the growth Stem diameter of the inoculated without fertilizer application was higher than the stem diameter of the non-inoculated without fertilizer application 14WAP (Table 6). Stem diameter of the non-inoculated significantly (p < 0.001) increased as the levels of PCMW and CMWFWSAU increased from 20 to 100 kg N ha -1 from 6WAP to 14WAP. Significantly (p < 0.001) higher stem diameter was observed between the inoculated and the non-inoculated at 40 and 100 kg N ha - 1 of PCMW; and 40 kg N ha -1 of CMWFWSAU 14WAP (Table 6). Significantly (p < 0.001) higher stem diameter was observed in the inoculated at 100 kg N ha - 1 of PCMW 14WAP. However, plant height of the inoculated without fertilizer application was 12.7% higher than the plant height of the non-inoculated without fertilizer application (Table 7). The higher the levels of PCMW and CMWFWSAU applied without mycorrhizal inoculation, the higher were the residual effect on the plant height with 100 kg N ha -1 of PCMW having significantly (p < 0.001) higher plant height 14WAP (Table 7). The percentage range of 3.5 to 9.0% was observed between the inoculated and noninoculated with fertilizers application 14WAP. Residual effects of AM and fertilizers on the colonization and yield of Tiannug 1 Core yield and mycorrhizal colonization were significantly (p < 0.05) affected by the residual effects of composted market waste application rates and mycorrhizal inoculation (Table 8). Inoculated Tiannug 1 at 0 fertilizer level was higher than the non-inoculated counterpart at the same fertilizer level by 10.6%. Increase in the levels of PCMW and CMWFWSAU without mycorrhizal inoculation resulted in the significant (p < 0.001) increase in bast yield at

5 100 kg N ha -1 of PCMW and CMWFWSAU. Inoculated Tiannug 1 had significantly (p < 0.001) higher bast yield than the noninoculated counterpart at 80 and 100 kg N ha -1 of PCMW, and also at 100 kg N ha -1 of CMWFWSAU (Tables 8). On the residual effect of PCMW and CMWFWSAU with mycorrhiza inoculation, significant (p < 0.001) increase were observed at 80 and 100 kg N ha -1 of both PCMW and CMWFWSAU. Inoculated Tiannug 1 at 0 fertilizer level was 22.1% higher than the noninoculated counterpart (Table 8). Significant (p < 0.001) increase was observed up to 80 kg N ha -1 of PCMW and CMWFWSAU. Significant increase was observed between the inoculated with fertilizer application and the non-inoculated counterpart at 100 kg N ha -1 of PCMW and CMWFWSAU. Inoculated was 7.2% higher than the non-inoculated (Table 8). Increase in the levels of PCMW and CMWFWSAU without mycorrhizal inoculation resulted in the significant (p < 0.001) increased in the mycorrhizal colonization from 60 to 100 kg N ha -1 of PCMW; and 80 to 100 kg N ha -1 of CMWFWSAU. Inoculated Tiannug1 at 60, 80 and 100 kg N ha -1 of PCMW and CMWFWSAU had significantly (p < 0.001) higher mycorrhizal colonization than their non-inoculated counterpart at the same fertilizer level. Table 1: Soil physical and chemical properties before planting. Soil properties Sand (g kg -1 ) Silt (g kg -1 ) Clay (g kg -1 ) Textural class ph (H 2 O) Organic matter (g kg -1 ) Total N (g kg -1 ) Available P (mg kg -1 ) K (cmol kg -1 ) Ca (cmol kg -1 ) Na (cmol kg -1 ) Mg (cmol kg -1 ) Exch.acidity (cmol kg -1 ) ECEC (cmol kg -1 ) Base saturation (%) Value Sandy loam Table 2: Proximate analysis of Composted Market Waste Fortified With Superphosphate And Urea (CMWFWSAU) and Purely Composted Market Waste (PCMW). Parameters CMWFWSAU PCMW Nitrogen (g kg -1 ) Phosphorus (mg kg -1 ) Potassium (cmol kg -1 ) Cacium (cmol kg -1 ) Magnesium(cmol kg -1 ) PCMW = Purely Composted Market Waste; CMWFWSAU = Composted Market Waste Fortified With Superphosphate And Urea 1155

6 Table 3: Effects of AM and fertilizers on the stem diameter (cm). Mycorrhiza inoculation Fertilizers application With NPK PCMW PCMW PCMW PCMW PCMW CMWFWSAU CMWFWSAU CMWFWSAU CMWFWSAU CMWFWSAU Without NPK PCMW PCMW PCMW PCMW PCMW CMWFWSAU CMWFWSAU CMWFWSAU CMWFWSAU CMWFWSAU SEM Mycorrhiza (M) Fertilizers (F) M x F ANOVA M *** *** *** *** *** F *** *** *** *** *** M x F *** *** *** *** *** Values are means of three replicates 6, 8, 10, 12 and 14 = Weeks after planting NPK60 = 60 kg N ha -1 of NPK (20:10:10) fertilizer PCMW20, PCMW40, PCMW60, PCMW80 and PCMW100 = 20, 40, 60, 80 and 100 kg N ha -1 of purely composted market waste. CMWFWSAU20, CMWFWSAU40, CMWFWSAU60, CMWFWSAU80 and CMWFWSAU100 = 20, 40, 60, 80 and 100 kg N ha -1 of composted market waste fortified with superphosphate and urea. SEM = Standard Error of Mean *** represent level of significance at p <

7 Table 4: Effects of AM and fertilizers on the plant height (cm). Mycorrhiza inoculation Fertilizers application With NPK PCMW PCMW PCMW PCMW PCMW CMWFWSAU CMWFWSAU CMWFWSAU CMWFWSAU CMWFWSAU Without NPK PCMW PCMW PCMW PCMW PCMW CMWFWSAU CMWFWSAU CMWFWSAU CMWFWSAU CMWFWSAU SEM Mycorrhiza (M) Fertilizers (F) M x F ANOVA M *** *** *** *** *** F *** *** *** *** *** M x F *** *** *** *** *** Values are means of three replicates 6, 8, 10, 12 and 14 = Weeks after planting NPK60 = 60 kg N ha -1 of NPK (20:10:10) fertilizer PCMW20, PCMW40, PCMW60, PCMW80 and PCMW100 = 20, 40, 60, 80 and 100 kg N ha -1 of purely composted market waste. CMWFWSAU20, CMWFWSAU40, CMWFWSAU60, CMWFWSAU80 and CMWFWSAU100 = 20, 40, 60, 80 and 100 kg N ha -1 of composted market waste fortified with superphosphate and urea SEM = Standard Error of Mean *** represent level of significance at p <

8 Table 5: Influence of AM and fertilizers on the colonization, bast and core yield. Mycorrhiza inoculation Fertilizers application Bast (g pot -1 ) Core (g pot -1 ) Mycorrhizal colonization (%) With NPK PCMW PCMW PCMW PCMW PCMW CMWFWSAU CMWFWSAU CMWFWSAU CMWFWSAU CMWFWSAU Without NPK PCMW PCMW PCMW PCMW PCMW CMWFWSAU CMWFWSAU CMWFWSAU CMWFWSAU CMWFWSAU SEM Mycorrhiza (M) Fertilizers (F) M F ANOVA M *** *** *** F *** *** *** M F *** *** *** Values are means of three replicates; NPK60 = 60 kg N ha -1 of NPK (20:10:10) fertilizer; PCMW20, PCMW40, PCMW60, PCMW80 and PCMW100 = 20, 40, 60, 80 and 100 kg N ha -1 of purely composted market waste; CMWFWSAU20, CMWFWSAU40, CMWFWSAU60, CMWFWSAU80 and CMWFWSAU100 = 20, 40, 60, 80 and 100 kg N ha -1 of composted market waste fortified with superphosphate and urea; SEM = Standard Error of Mean ; *** represent level of significance at p <

9 Table 6: Residual effects of AM and fertilizers on the stem diameter (cm). Mycorrhiza inoculation Fertilizers application With NPK PCMW PCMW PCMW PCMW PCMW CMWFWSAU CMWFWSAU CMWFWSAU CMWFWSAU CMWFWSAU Without NPK PCMW PCMW PCMW PCMW PCMW CMWFWSAU CMWFWSAU CMWFWSAU CMWFWSAU CMWFWSAU SEM Mycorrhiza (M) Fertilizers (F) M x F ANOVA M *** ** ns ns ns F *** *** *** *** *** M x F *** *** *** *** *** Values are means of three replicates 6, 8, 10, 12 and 14 = Weeks after planting NPK60 = 60 kg N ha -1 of NPK (20:10:10) fertilizer PCMW20, PCMW40, PCMW60, PCMW80 and PCMW100 = 20, 40, 60, 80 and 100 kg N ha -1 of purely composted market waste. CMWFWSAU20, CMWFWSAU40, CMWFWSAU60, CMWFWSAU80 and CMWFWSAU100 = 20, 40, 60, 80 and 100 kg N ha -1 of composted market waste fortified with superphosphate and urea SEM = Standard Error of Mean ** and *** represent level of significance at p < 0.01 and respectively ns = not significant 1159

10 Table 7: Residual effects of AM and fertilizers on the plant height (cm). Mycorrhiza Fertilizers inoculation application With NPK PCMW PCMW PCMW PCMW PCMW CMWFWSAU CMWFWSAU CMWFWSAU CMWFWSAU CMWFWSAU Without NPK PCMW PCMW PCMW PCMW PCMW CMWFWSAU CMWFWSAU CMWFWSAU CMWFWSAU CMWFWSAU SEM Mycorrhiza (M) Fertilizers (F) M x F ANOVA M *** *** *** *** *** F *** *** *** *** *** M x F * *** *** ** ns Values are means of three replicates 6, 8, 10, 12 and 14 = Weeks after planting NPK60 = 60 kg N ha -1 of NPK (20:10:10) fertilizer PCMW20, PCMW40, PCMW60, PCMW80 and PCMW100 = 20, 40, 60, 80 and 100 kg N ha -1 of purely composted market waste. CMWFWSAU20, CMWFWSAU40, CMWFWSAU60, CMWFWSAU80 and CMWFWSAU100 = 20, 40, 60, 80 and 100 kg N ha -1 of composted market waste fortified with superphosphate and urea SEM = Standard Error of Mean *, ** and *** represent level of significance at p < 0.05, 0.01 and respectively ns = not significant 1160

11 Table 8: Residual effect of AM and fertilizers on the colonization, bast and core yield Mycorrhiza inoculation Fertilizers application Bast (g pot -1 ) Core (g pot -1 ) Mycorrhizal colonization (%) With NPK PCMW PCMW PCMW PCMW PCMW CMWFWSAU CMWFWSAU CMWFWSAU CMWFWSAU CMWFWSAU Without NPK PCMW PCMW PCMW PCMW PCMW CMWFWSAU CMWFWSAU CMWFWSAU CMWFWSAU OM SEM Mycorrhiza (M) Fertilizers (F) M F ANOVA M *** *** *** F *** *** *** M F ns *** * Values are means of three replicates NPK60 = 60 kg N ha -1 of NPK (20:10:10) fertilizer PCMW20, PCMW40, PCMW60, PCMW80 and PCMW100 = 20, 40, 60, 80 and 100 kg N ha -1 of purely composted market waste. CMWFWSAU20, CMWFWSAU40, CMWFWSAU60, CMWFWSAU80 and CMWFWSAU100 = 20, 40, 60, 80 and 100 kg N ha -1 of composted market waste fortified with superphosphate and urea SEM = Standard Error of Mean * and *** represent level of significance at p < 0.05 and respectively ns = not significant 1161

12 DISCUSSION The soil used for this study was low in nutrients compared to the soil fertility rating classes in Nigerian (FPDD, 1990). Low fertility status of the soil might be due to the continuous cultivation of the land with continuous application of inorganic fertilizer in the past. The infective propagule density was low, less than 140 infective propagules per 100 g soil (Sieverding, 1991). Tiannug 1 showed a higher degree of responsiveness to mycorrhizal inoculation. Variation in response to mycorrhizal inoculation has been obtained in different species and genotypes of other crops (Dare et al., 2008). However, inoculation increased the growth, AM colonization, bast and core yield of Tiannug 1 compared to the non-inoculation counterpart. These better growth exhibited by the inoculated might be due to a better uptake of nutrients, which in turn can be directly attributed to AM inoculation. This might also be due to the effectiveness of Glomus mosseae inoculated to absorb plant nutrient from soil solution for the growth and yield of the plant. Increase in the levels of the compost applied with mycorrhizal inoculation significantly (p < 0.001) increased mycorrhizal colonization, bast and core yield from 20 to 40 kg N ha -1 of both PCMW and CMWFWSAU. High amount of soil available P and total N may lower AM colonization (Treseder and Allen, 2002; Johnson et al., 2003). On the residual effect of AM inoculation and compost application, percentage AM colonization, growth and yield parameters increased from 20 kg N ha -1 to 100 kg N ha -1 of both PCMW and CMWFWSAU. PCMW had better yields than CMWFWSAU. This might be due to the gradual release of nutrients by the PCMW which make it to have better residual effect among the compost used. Conclusion Tiannug 1 was colonized by arbuscular mycorrhiza. Organic base fertilizer had higher percentage mycorrhizal colonization than the recommended rate of NPK (20: 10: 10) fertilizer. Percentage root colonization decreased as the nitrogen level increased. The higher the percentage root colonization, the higher were the growth (stem girth and plant height) and yield (bast and core) parameters. Inoculated Tiannug 1 at 40 kg N ha -1 of CMWFWSAU had significantly higher mycorrhizal colonization, bast and core yield. On the residual effects of fertilizers application and mycorrhizal inoculation, inoculated Tiannug 1 of PCMW had significantly higher mycorrhizal colonization, bast and core yield. For sustainable kenaf production, 40 kg N ha -1 of organic fertilizer along with arbuscular mycorrhiza is recommended. However, there is need to manage indigenous mycorrhiza and organic matter content of the soil to reduce input of chemical fertilizer through the application of organic material. REFERENCES Atayese MO, Awotoye OO, Osonubi O, Mulongoy K Comparisons of the influence of vesicular arbuscular mycorrhizal on the productivity of hedgerow woody legumes and cassava at the top and the base of a hillslope in alley cropping systems. Biology and Fertility of Soils, 16: Bada BS, Kalejaiye ST Response of kenaf (Hibiscus cannabinus L.) grown in different soil textures and lead concentrations. Research Journal of Agriculture and Biological Sciences, 6(5): Bada BS, Olatunde AK, Taiwo DA Heavy metal content of soil and jute mallow collected from organic and 1162

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