Flower Seed Testing Committee. Chair: Rita Zecchinelli Vice-Chair: Sharon Davidson

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1 Flower Seed Testing Committee Chair: Rita Zecchinelli Vice-Chair: Sharon Davidson

2 Contents 1. Committee Report 2012/ Committee Working Program

FSC COMPOSITION 2010-13 Chair Rita Zecchinelli Italy Vice-Chair Sharon K. Davidson USA Member 1 Aleta Meyr * USA Member 2 Anton Grim The Netherlands Member 3 Frikkie A.

3 FSC COMPOSITION Chair Rita Zecchinelli Italy Vice-Chair Sharon K. Davidson USA Member 1 Aleta Meyr * USA Member 2 Anton Grim The Netherlands Member 3 Frikkie A. Marais South Africa Member 4 Kumiko Jinno Japan Member 5 Meriam Dekalo Keren Israel Member 6 Dusica Jovicic* Serbia Member 7 Sylvie Ducournau France Member 8 Wen-shi Wu* Member 9 Zita Ripka Hungary Separate Customs Territory of Taiwan, Penghu, Kinmen and Matsu * Membership not confirmed

4 Working group ISTA Flower Seed Testing Handbook Chair: Zita Ripka Anton Grim Frikkie Marais Meriam Dekalo Sharon Davidson Sylvie Ducournau Wen-shi Wu

5 PREPARATION OF WORKSHEETS 1. Questionnaire circulated within FSC for the genus/species 2. Evaluation of answers 3. Draft work sheet 4. Circulation within FSC corrections 5. Final version circulation within ISTA committees (PUR, GER,TEZ) 6. Final editing of work sheet - Jonathan Taylor ISTA

6 ISTA Handbook on Flower Seed Testing 2008 issue of 1st Edition (24) 2010 Supplement issued with work sheets (+7) New work sheets prepared (31 finalized) 2 NEW WORK SHEETS WILL BE SOON DISTRIBUTED

7 WORK SHEETS BY GENUS (16 finalized in 2010) Abutilon x hybridum Alcea rosea Althaea spp.(2) Alyssum spp. (2) Arabis spp.(5) Aubrieta deltoidea Aurinia saxatilis Campanula spp. (10) Erysimum spp. (2) Hesperis matronalis Iberis spp. (4) Lavatera trimestris Lobularia maritima Lunaria annua Malope trifida Limonium spp. (4) Total 38 species

8 WORK SHEETS BY GENUS (9 finalized in 2011) Lobelia spp. (2) Amaranthus spp. (3) Aquilegia spp. (5) Convolvulus tricolor Ipomoea spp. (2) Papaver spp. (5) Chrysanthemum indicum Glebionis spp. (3) Leucanthemum spp. (2) Tanacetum spp. (3) Total 22 species

9 WORK SHEETS BY GENUS (6 finalized in 2012) Achillea spp. (4) Adonis vernalis Ranunculus asiaticus Anemone spp. (3) Clarkia spp. (3) Cleome hassleriana Oenothera macrocarpa Total 17 species Lathyrus spp. (2) Lupinus spp. (4) Mimosa pudica Linum spp. (4) Problems: No images of seedlings (Some seed images are missing)

12 ISTA Handbook on Flower Seed Testing 19% 45% 10% 6% 7% 8% 5% started herbs rest Total: 353 species listed in Table 2A Part3

13 WORK SHEETS: COOPERATION ISTA Germination Committee ISTA Tetrazolium Committee ISTA Purity Committee in seed-borne diseases Prof. Wen-Shi Wu Jonathan Taylor in editing from the ISTA Secretariat MANY THANKS TO PROF. WEN-SHI WU and all best wishes to his retired years

14 Incidence of the root system on the evaluation of seedling development of Salvia spp. Sylvie Ducournau, Valérie Blouin (GEVES-SNES, FR)

15 The experiment Objective - Evaluate the incidence of the absence of the primary root on the seedling development of Salvia spp., and depending on the results to propose a change of the seedling group for the evaluation of this species. Material and methods - 7 samples of Salvia spp. - Germination on top of paper, at a constant temperature of 20 C with counts after 7 and 14 days - Normal seedlings and abnormal seedlings with defective primary root and various number of secondary roots are selected and transplanted in organic growing media for 60 more days - Measurements and evaluation of plants Global development Plant height

16 Development of the plants depending on the seedling root system 20 Plant height (cm) Normal seedling No secondary root 1 secondary root 2 secondary roots 3 secondary roots >3 secondary roots Except for seedlings with no secondary root or with only one secondary root having no statistical data, the shoot height of normal seedling is not significantly different from the shoot height of seedlings with defective primary root with at least 2 secondary roots.

17 Development of the plants depending on the seedling root system Seedling #3 with defective primary root and 2 secondary roots Plants from seedling #3 (left) and from normal seedling (right) after 60 days

18 Conclusion Even if seedlings have defective primary root system, and therefore abnormal according to the current classification, they develop into plants that are comparable to those developed from normal seedlings, after transplanting. This support a proposal for changing the ISTA Seedling Evaluation Group of Salvia spp. from A (primary root required without consideration of secondary roots) to the Group A (primary root not required if the secondary roots are sufficient).

Lupinus polyphyllus (flower) Lupinus albus (vegetable) NO any secondary

19 Project: seedling evaluation of flower species of Lupinus Type F - Seedling Group A : secondary roots soon produced? Lupinus polyphyllus (flower) Lupinus albus (vegetable) NO any secondary roots produced (or only a few and very late) L. polyphyllus vs L. albus YES secondary roots produced

20 Evaluation of the role of the primary root in the flower species of Lupinus Lupinus angustifolius Before cutting After cutting Lupinus polyphyllus Removal of the radicle tip Working group Anton Grim Frikkie Marais Rita Zecchinelli

any/significant numbers of secondary roots in method BP during the incubation period.

21 Evaluation of the role of the primary root in the flower species of Lupinus Lupinus hartwegii Before cutting After cutting Preliminary observation Ornamental Lupinus does not seem to form any/significant numbers of secondary roots in method BP during the incubation period. It confirms the previous observations (substrates: S/ TP). Some secondary roots did form when the primary root was damaged.

22 Experiment on a revised method for the germination test of Alcea rosea. Test organizer: Valérie Blouin, GEVES-SNES (FR) Objective: add sand as an additional substrate to the ISTA germination methods for Alcea rosea. Observations have shown that germination in sand provides better results than germination in paper substrate. The root system is easier to evaluate for the assessment of normal and abnormal seedlings. Participants: Valérie Blouin (France) Anton Grim (The Netherlands) Frikkie Marais (South Africa) Meriam Dekalo Keren (Israel) Sharon Davidson (USA) Zita Ripka (Hungary) Status: The tests have been concluded. The results are under evaluation

23 Revision of Table 2A part 3 Flower, spice, herb and medicinal species

24 Revision of Table 2A part 3 Flower, spice, herb and medicinal species Data already available: - 3 countries species samples

25 2. Committee Working Programme ISTA Handbook on Flower Seed Testing: future plans FLOWER SPECIES: HERBS: Asparagus (2) Consolida (2) Digitalis (2) Echium (2) Helipterum (3) Kalanchoe (3) 1 Linaria (3) 1 Mimulus (4) 1 Myosotis (3) 1 Nemesia (2) 1 Nemophila (2) Nicotiana (3) 2 Nigella (3) 2 Penstemon (3) 2 Phlox (3) 2 Saponaria (3) 2 Scabiosa (2) Senecio (3) 2 Silene (3) Solanum (4) 1 Tripleurospermum (2) 1 Verbascum (3) 1 Angelica archangelica Arthemisia spp (4) Cnicus benedictus Datura spp. (2) Echinacea purpurea Hyssopus officinalis Lavandula angustifolia Marrubium vulgare Matricaria recutita Melissa officinalis Menths x piperita Perilla frutescens Pimpinella spp. (2) Reseda odorata Ruta graveolens Thymus serpillum Sylibum marianum Valeriana officinalis

2. Committee Working Programme 2013-2016 A new species in the ISTA Rules: Felicia heterophylla Introduction of a new species in the ISTA Rules: Felicia heterophylla Validation study of the

26 2. Committee Working Programme A new species in the ISTA Rules: Felicia heterophylla Introduction of a new species in the ISTA Rules: Felicia heterophylla Validation study of the germination method carried out in 2012/13: UNSUCCESSFUL!! CONCLUSION (Jean-Luis Laffont ISTA Statistics Committee) None of the four methods fulfill the repeatability and the reproducibility criteria. Best compromise is reached for method 20-None METHODS TP; C (first count 4-7 days; final count: 21 days). Additional treatment: NONE TP; C (first count 4-7 days; final count: 21 days). Additional treatment: Prechill: 7 days at 5 o C + 0,2% KNO 3 solution (applied together) TP; 20 C (first count 4-7 days; final count: 21 days). Additional treatment: NONE TP; 20 C (first count 4-7 days; final count: 21 days). Additional treatment: Prechill: 7 days at 5 o C + 0,2% KNO 3 solution (applied together)

2. Committee Working Programme 2013-2016 A new species in the

looking for new seed lots in order to ri-organize the

27 2. Committee Working Programme A new species in the ISTA Rules: Felicia heterophylla Photo: Zita Ripka We are looking for new seed lots in order to ri-organize the validation study and finally have Felicia heterophylla included in the ISTA Rules

2. Committee Working Programme 2013-2016

28 2. Committee Working Programme A new species in the ISTA Rules: Passiflora spp. We have received the proposal to introduce Passiflora in the Rules: - we are looking for seeds - we are looking for information (species, germination methods)

29 2. Committee Working Programme ISTA Proficiency Test plan 2013/2016: Callistephus chinensis (GER) Test Leader: Zita Ripka Test round: PT 14-1

30 WORKSHOP A Flower Seed Testing Workshop is planned (2015?)

31 THANKS TO THE FSC MEMBERS FOR THE WORK DONE! THANKS TO THE SECRETARIAT FOR THE SUPPORT! THANK YOU FOR YOUR ATTENTION!

ISTA FLOWER SEED TESTING COMMITTEE. Rita Zecchinelli

ISTA FLOWER SEED TESTING COMMITTEE Rita Zecchinelli Agenda Committee membership 2013-2016 Committee membership 2016-2019 Working programme 2013 2016: summary report Working programme 2016-2019: activities