EDUCATION 2012 Postdoctoral fellow Johns Hopkins School of Medicine, Baltimore, MD

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1 Hani Zaher Washington University in St. Louis, Department of Biology, Campus Box 1137, One Brookings Drive St. Louis, MO Tel: Fax: RESEARCH INTERESTS I am interested in the functional role of RNA in metabolism. Recently my research has focused on understanding the mechanisms that govern translational fidelity and its impact on cellular fitness and codon evolution. My future goals are to expand our understanding of translation- based quality control mechanisms in eukaryotes and their impact on gene regulation CURRENT POSITION Assistant of Biology Washington University in St. Louis EDUCATION 2012 Postdoctoral fellow Johns Hopkins School of, Baltimore, MD 2007 Ph.D. in Molecular Biology and Biochemistry (Supervisor: Peter Unrau), Simon Fraser University, Burnaby, BC, Canada 2002 B.Sc. in Molecular Biology and Biochemistry (co- operative education), Simon Fraser University, Burnaby, BC, Canada HONORS/AWARDS 2011 The RNA Society/Scaringe Young Scientist Award K99/R00 NIH Pathway to Independence Award Postdoctoral Fellowship: Natural Sciences and Engineering Research Council of Canada 2007 Best graduate thesis in the Department of Molecular Biology and Biochemistry: Simon Fraser University 1

2 2007 Graduate Fellowship: Simon Fraser University 2006 First Poster Prize: GRC, Origin of Life meeting 2006 President s Fellowship: Simon Fraser University 2006 Graduate Fellowship: Simon Fraser University Doctoral Award: Canadian Institutes of Health Research 2004 Graduate Fellowship: Simon Fraser University 2004 Graduate Fellowship: Simon Fraser University 1998 Open Scholarship: Simon Fraser University Summit Entrance Scholarship: Simon Fraser University United World College Scholarship: Lester B. Pearson College of the Pacific PUBLICATIONS Petropoulos, A.D., Green, R. and Zaher, H. S. Biochemical dissection of the role of bacterial release factors in translational quality control. In preparation. Zhang, B., Pierson, W. and Zaher, H.S. Molecular basis for the inhibitory effects of adenosine- inosine codon- anticodon interaction in yeast. In preparation. Zaher, H. S. and Green, R. (2011). A primary role for release factor 3 in quality control during translation elongation in Escherichia coli. Cell, 147(2): Zaher, H. S. and Green, R. (2011). A primary role for release factor 3 in quality control during translation elongation in Escherichia coli. Cell, 147(2): Miller, M. R., Liu, Z., Cazier, D. J., Gebhard, G. M., Herron, S. R., Zaher, H. S., Green, R. and Buskirk, A. R. (2011). The role of SmpB and the ribosomal decoding center in licensing tmrna entry into stalled ribosomes. RNA, 17(9): Zaher, H. S., Shaw, J. J., Strobel, S. A. and Green, R. (2011). The 2'- OH group of the peptidyl- trna stabilizes an active conformation of the ribosomal PTC. EMBO J, 30(12): Ortiz- Meoz, R. F., He, S. L., Zaher, H. S. and Green, R. (2011). Sense and nonsense recognition by the ribosome in Ribosomes Structure, Function, and Dynamics, Rodnina, M. V.; Wintermeyer, W.; Green, R., eds. (Springer- Verlag, New York). (Book Chapter) Zaher, H. S. and Green, R. (2010). Kinetic basis for global loss of fidelity arising from mismatches in the P- site codon:anticodon helix. RNA, 16(10): Zaher, H.S. and Green, R. (2010). Hyperaccurate and error- prone ribosomes exploit distinct mechanisms during trna selection. Molecular Cell, 39(1):

3 Zaher, H. S. and Green, R. (2009). Quality control on the ribosome following peptide bond formation. Nature, 457(7226): Zaher, H. S. and Green, R. (2009). Fidelity at the molecular level: lessons from protein synthesis. Cell, 136(4): (Review) Zaher, H. S. and Unrau, P. J. (2007). Selection of an improved RNA polymerase ribozyme with superior extension and fidelity. RNA, 13(7): Zaher, H. S. and Unrau, P. J. (2006). A general RNA- capping ribozyme retains stereochemistry during cap exchange. JACS, 128(42): Zaher, H. S., Watkins, R. A. and Unrau, P. J. (2006). Two distinct capping ribozymes have highly convergent catalytic properties. RNA, 12(11): Zaher, H. S. and Unrau, P. J. (2005). Nucleic acid library construction using synthetic DNA constructs. Methods Mol Biol. 288: (Book Chapter) Zaher, H. S. and Unrau, P. J. (2004). T7 RNA polymerase mediates fast promoter- independent extension of unstable nucleic complexes. Biochemistry 43(24): FUNDING NIH K99/R00 (K99GM R00GM094210) Pathway to Independence Award, H Zaher (PI) The molecular mechanism of post- peptidyl transfer quality control on the ribosome INVENTIONS Zaher, H. S. and Green, R. Manipulation of post peptidyl transfer quality Control for protein synthesis. ORAL PRESENTATIONS Mechanism and implications of proofreading on the ribosome Biochemistry/Biophysics retreat, Washington University in St. Louis, (October 2013). (Keynote) Mechanism and implications of proofreading on the ribosome Simon Fraser University, BC, Canada (February 2013). (Invited talk) 3

4 Dealing with mistakes: the ribosome s response to misincorporations Gordon Research Conference, DNA Damage, Mutation & Cancer, Ventura, California (March 2010). (Invited talk) RNA- mediated chemistries: a case of replication and capping Darwin s Legacy Conference, Hamilton, Ontario, Canada (May 2009). (Keynote address) Dealing with mistakes: the ribosome s response to misincorporations New York Academy of Science, New York City, New York (April 2009). (Invited talk) Dealing with mistakes: the ribosome s response to misincorporations The Philadelphia RNA club, Philadelphia, Pennsylvania (March 2009). (Invited talk) RF2 accelerates peptide release on sense codons after a miscoding event RNA Society 13 th Annual Meeting, Berlin, Germany (July 2008). (Abstract- selected talk) Processive and Accurate Polymerization by an Improved RNA Polymerase Ribozyme RNA Society 12 th Annual Meeting, Madison, Wisconsin (June 2007). (Abstract- selected Talk) Improved RNA- catalyzed RNA polymerization: towards an RNA replicase ribozyme Volcano Conference in Bioorganic Chemistry, Pack Forrest, Washington (Feb. 2007). A general RNA- capping ribozyme retains stereochemistry during cap formation. RNA Society 11 th Annual Meeting, Seattle, Washington (June 2006). (Abstract- selected Talk) In vitro selection and characterization of a general capping ribozyme RNA Society 10 th Annual Meeting, Banff, Alberta (May 2005). (Abstract- selected Talk) Fast T7 RNA polymerase mediated extension of transient nucleic acid complexes Volcano Conference in Bioorganic Chemistry, Pack Forrest, Washington (Feb. 2004). RNA world and ribozyme chemistry MBB Annual Colloquium, Simon Fraser University, Burnaby, BC, Canada (Jan. 2003). SELECTED ABSTRACTS/POSTERS Zaher, H.S. Towards understanding the inhibitory effects of codon- anticodon wobble base pairing on the ribosome Ribosomes 2013 Meeting, Napa, CA (July 2013). 4

5 Zaher, H.S. Towards understanding the inhibitory effects of codon- anticodon wobble base pairing on the ribosome RNA Society 18 th Annual Meeting, Davos, Switzerland (July 2013). Zaher, H. S. and Green, R. A critical role for release factor 3 in quality control during translation elongation in Escherichia coli RNA Society 16 th Annual Meeting, Kyoto, Japan (June 2011). Zaher, H. S. and Green, R. (2011). A critical role for release factor 3 in quality control during translation elongation in Escherichia coli Ribosomes 2010 Meeting, Orvieto, Italy (May 2010). Zaher, H. S., Le Fevre, T. and Unrau, P. J., Sequence and Structure Requirements for the Replication of the Viroid- like RNA X by T7 RNA Polymerase RNA Society 12 th Annual Meeting, Madison, Wisconsin (June 2007). Zaher, H. S. and Unrau, P. J. Direct selection of an improved trans- acting RNA polymerase ribozyme by in vitro compartmentalization Gordon Research Conference, Origin of Life, Lewiston, Maine (July 2006). Zaher, H. S. and Unrau, P. J., T7 RNA polymerase mediates fast promoter independent extension of unstable nucleic acid complexes RNA Society 9 th Annual Meeting, Madison, Wisconsin (Jun. 2004). Zaher, H. S. and Unrau, P. J., One base- pair DNA- template dependent RNA polymerization by T7 RNA polymerase Ribo- Club, Sherbrooke, Quebec (Sep. 2003). TEACHING SP12 SP13 SP13 BIO548 Nucleic Acids and Protein Synthesis (Discussion leader) BIO548 Nucleic Acids and Protein Synthesis (One of 6 lecturers) BIO451 General Biochemistry, Washington University in St. Louis MENTORING ACTIVITIES Postdoctoral fellow: Benjamin Hudson (2013- ) Undergraduate Students: Ali Rangwala (2012- ) Jessica Qiu (2013- ) Will Pierson (2013- ) Technicians Bo Zhang (2012- ) 5

6 John Mosior (2013- ) EMPLOYMENT Postdoctoral fellow Johns Hopkins School of, Baltimore, MD Graduate student Simon Fraser University, Burnaby, BC, Canada Thesis Title: RNA- mediated chemistries: a case of replication and capping Teaching assistant, Physical Biochemistry Simon Fraser University, Burnaby, BC, Canada Research assistant Canadian Inovatech, Abbotsford, BC, Canada 2000 Validation chemist (Co- op Student) Vita Health, North Vancouver, BC, Canada 1999 Quality Control Technician (Co- op Student) Agrevo Canada, Saskatoon, SK, Canada 1998 Quality Control Technician (Co- op Student) Natural Factors, Burnaby, BC, Canada OTHER RELATED ACTIVITIES Member of the steering committee for the Biochemistry Program in the Division of Biological & Biomedical Sciences (DBBS) at Washington University in St. Louis. Reviewer for NASA Astrobiology Omics/Evolution review panel. Reviewer for RNA, JBC, Nature communications, PNAS, Nature Biotechnology and Journal of Molecular Evolution. 6

7 REFERENCES Rachel Green, Ph.D. Investigator, Howard Hughes Medical Institute Genetics Phone: e- mail: Peter Unrau, Ph.D. Associate Biochemistry, Simon Fraser University Phone: e- mail: Geraldine Seydoux, Ph.D. Investigator, Howard Hughes Medical Institute Genetics Phone: , e- mail: Jeffry L. Corden, Ph.D. Genetics Phone: , e- mail: Dipankar Sen, Ph.D. Biochemistry, Simon Fraser University Phone: e- mail: Jon Lorsch, Ph.D. Biophysics & Biophysical Chemistry Phone: e- mail: 7