Augen A. Pioszak, Ph.D.

Transcription

1 Assistant Professor University of Oklahoma Health Sciences Center 975 N.E. 10 th Street, BRC 462B P.O. Box Oklahoma City, OK Phone: (405) Fax: (405) EDUCATION and TRAINING August 2005 July 2010 Postdoctoral Fellow, Van Andel Research Institute, Grand Rapids, MI Advisor: Dr. H. Eric Xu Structural Biology of G Protein-coupled Receptors April 2004 July 2005 Postdoctoral Fellow, RIKEN Yokohama Institute, Yokohama, JAPAN Advisor: Dr. Shigeyuki Yokoyama Protein X-ray Crystallography August 1997 March 2004 Graduate Student, Department of Biological Chemistry, University of Michigan, Ann Arbor, MI Ph.D. in Biological Chemistry, August 2003 Dissertation Advisor: Dr. Alexander J. Ninfa Structure and Function of Bacterial Two-component Signal Transduction Proteins August 1994 May 1997 Undergraduate Student, Biochemistry, Michigan State University, East Lansing, MI B.S. in Biochemistry, May 1997 Senior Thesis Advisor: Dr. Zachary F. Burton Structure and Function of the TFIIF and TFIIB General Transcription Factors PROFESSIONAL EXPERIENCE Academic August 2010 present Assistant Professor,, University of Oklahoma Health Sciences Center, Oklahoma City, OK April 2011 present Member, Peggy and Charles Stephenson Oklahoma Cancer Center, Basic Cancer Biology Research Program, University of Oklahoma Health Sciences Center, Oklahoma City, OK SCHOLARSHIP Research Funding Current RAMP-altered Class B GPCR Hormone Recognition Role: PI, 35% effort NIH 1R01GM $190,000 Direct Costs/yr, 5 yrs. 09/26/12 08/31/2017 1

2 Completed Structure and Function of the Human Calcitonin Receptor Role: PI, 20% effort OUHSC Office of the Vice President for Research Seed Grant $30,000 Direct Costs/yr, 1 yr 04/01/ /31/2012 Hormone Recognition by the Extracellular Domain of the Calcitonin Receptor Role: PI, 20% effort OUHSC College of Medicine Alumni Association (COMAA) Research Fund Seed Grant $30,000 Direct Costs/yr, 1 yr 07/01/ /30/2012 Modulation of Class B GPCR Hormone-Binding by RAMPs Role: PI, 25% effort Oklahoma Center for the Advancement of Science and Technology (OCAST) Health Research Program HR $45,000 Direct Costs/yr, 3 yrs. 07/01/ /30/2014 Terminated 09/25/2012 to accept NIH 1R01GM Hormone Recognition by the Calcitonin and Amylin Receptors Role: Junior PI, 50% effort NIH COBRE 1P20GM Parent Grant: Oklahoma Center of Biomedical Research Excellence (COBRE) in Structural Biology PI: Ann West $150,000 Direct Costs/yr, 5 yrs. [for junior PI proposal] 08/01/ /31/2017 Terminated 09/25/2012 to accept NIH 1R01GM Scholarly Activities and Creative Achievements Patents Parathyroid Hormone Peptides and Parathyroid Hormone-Related Protein Peptides and Methods of Use Application No.: PCT/US2010/28867 Filing Date: March 26, 2010 Role: Co-inventor resulting from Postdoctoral Research at the Van Andel Research Institute PUBLICATIONS * Indicates mentor Peer Reviewed 1. Hill, H. E., and A. A. Pioszak. (2012). Bacterial expression and purification of a heterodimeric adrenomedullin receptor extracellular domain complex using DsbC-assisted disulfide shuffling. Protein Expr. Purif. 88(1),

3 2. Kumar, S., Pioszak, A. A., Zhang, C., Swaminathan, K., and H. E. Xu. (2011). Crystal structure of the PAC1R extracellular domain unifies a consensus fold for hormone recognition by class B G-protein coupled receptors. PLoS ONE 6(5): e Pal, K., Swaminathan, K., Xu, H. E., and A. A. Pioszak*. (2010). Structural basis for hormone recognition by the human CRFR2α G protein-coupled receptor. J. Biol. Chem. 285, Pioszak, A. A., Harikumar, K. G., Parker, N. R., Miller, L. J., and H. E. Xu. (2010). Dimeric arrangement of the parathyroid hormone receptor and a structural mechanism for ligand-induced dissociation. J. Biol. Chem. 285, [co-corresponding author] 5. Pioszak, A. A., Parker, N. R., Gardella, T. J., and H. E. Xu. (2009). Structural basis for parathyroid hormone-related protein binding to the parathyroid hormone receptor and design of conformation-selective peptides. J. Biol. Chem. 284, [co-corresponding author] 6. Pioszak, A. A., Parker, N. R., Suino-Powell, K., and H. E. Xu. (2008). Molecular recognition of corticotropin-releasing factor by its G protein-coupled receptor CRFR1. J. Biol. Chem. 283, [co-corresponding author] 7. Pioszak, A. A., and H. E. Xu. (2008). Molecular recognition of parathyroid hormone by its G protein-coupled receptor. Proc. Natl. Acad. Sci. USA. 105, Jiang, P., Pioszak, A. A., and A. J. Ninfa. (2007). Structure-function analysis of glutamine synthetase adenylyltransferase (ATase, EC ) of Escherichia coli. Biochemistry. 46, Pioszak, A. A., Murayama, K., Nakagawa, N., Ebihara, A., Kuramitsu, S., Shirouzu, M., and S. Yokoyama. (2005). Structures of a putative RNA 5-methyluridine methyltransferase, Thermus thermophilus TTHA1280, and its complex with S-adenosyl-L-homocysteine. Acta Cryst. F61, Pioszak, A. A., and A. J. Ninfa. (2004). Mutations altering the N-terminal receiver domain of NRI (NtrC) that prevent dephosphorylation by the NRII-PII complex in Escherichia coli. J. Bacteriol. 186, Song, Y., Peisach, D., Pioszak, A. A., Xu, Z., and A. J. Ninfa. (2004). Crystal structure of the C- terminal domain of the two-component system transmitter protein nitrogen regulator II (NRII; NtrB), regulator of nitrogen assimilation in Escherichia coli. Biochemistry. 43, Pioszak, A. A., and A. J. Ninfa. (2003). Mechanism of the PII-activated phosphatase activity of Escherichia coli NRII (NtrB): How the different domains of NRII collaborate to act as a phosphatase. Biochemistry. 42,

4 13. Pioszak, A. A., and A. J. Ninfa. (2003). Genetic and biochemical analysis of phosphatase activity of Escherichia coli NRII (NtrB) and its regulation by the PII signal transduction protein. J. Bacteriol. 185, Pioszak, A. A., Jiang, P., and A. J. Ninfa. (2000). The Escherichia coli PII signal transduction protein regulates the activities of the two-component system transmitter protein NRII by direct interaction with the kinase domain of the transmitter module. Biochemistry. 39, Book Chapters 1. Jiang, P., Pioszak, A. A., Atkinson, M. R., Peliska, J. A., and A. J. Ninfa. (2003). New insights into the mechanism of the kinase and phosphatase activities of Escherichia coli NRII (NtrB) and their regulation by the PII protein, in Histidine Kinases in Signal Transduction (Inouye, M. and Dutta, R, Eds.) pp , Academic Press, San Diego, CA Abstracts Local 1. Hill, H. E., and A. A. Pioszak. Structural Biology of the Human Adrenomedullin and CGRP Receptors. OU Cancer Center Retreat. Oklahoma City, OK. November (poster presentation) National 1. Pioszak, A. A., Harikumar, K. G., Parker, N. R., Miller, L. J., and H. E. Xu. Dimeric arrangement of the parathyroid hormone receptor and a structural mechanism for ligand-induced dissociation. Keystone GPCR Symposium. Breckenridge, CO. April (poster presentation) 2. Pioszak, A. A., Parker, N. R., Gardella, T. J., and H. E. Xu. Structural basis for parathyroid hormone-related protein binding to the parathyroid hormone receptor and design of conformation-selective peptides. Gordon Research Conference, Phosphorylation & G-Protein Mediated Signaling Networks. Biddeford, Maine. June (poster presentation) 3. Pioszak, A. A., and H. E. Xu. Molecular recognition of parathyroid hormone by the extracellular domain of its G protein-coupled receptor, PTH1R. Keystone GPCR Symposium. Killarney, Ireland. May (poster presentation) 4. Pioszak, A. A., and A. J. Ninfa. Mechanism of the PII-activated phosphatase activity of the Escherichia coli histidine kinase NRII (NtrB). Experimental Biology 2003 (ASBMB). San Diego, CA. April (oral and poster presentations) 5. Pioszak, A. A., Jiang, P., and A. J. Ninfa. The Escherichia coli PII signal transduction protein regulates the activities of the two-component system transmitter protein NRII (NtrB) by direct interaction with the kinase domain of the transmitter module. Molecular Genetics of Bacteria and Phages. Madison, WI. August (poster presentation) PRESENTATIONS Local Structural Biology of Peptide Hormone Recognition by Class B G Protein-coupled Receptors 4

5 University of Oklahoma, Department of Chemistry and Biochemistry Norman, OK March 28, 2011 Structural Biology of Class B G Protein-coupled Receptors OUHSC, Department of Physiology Oklahoma City, OK October 18, 2012 TEACHING Course Participation Department Spring Semester, 2013 Molecular Structure and Graphics in the Biomedical Sciences (BIOC 6321) Lecture Structure Superpositions 1.5 hrs Lecture Ligand Docking and Designing Mutants 1.5 hrs GTPases as Biological Switches (BIOC ) Lecture Heterotrimeric G proteins I 1.5 hrs Lecture Heterotrimeric G proteins II 1.5 hrs Fall Semester, 2012 Physical Biochemistry of Macromolecules (BIOC 6214) Lecture Binding Affinity and Cooperativity 1.5 hrs Lecture Rates of Binding 1.5 hrs Lecture Transition State Theory and Reaction Mechanisms 1.5 hrs Classroom Student Presentations 3 hrs Spring Semester, 2012 Molecular Structure and Graphics in the Biomedical Sciences (BIOC 6321) Lecture Structure Superpositions 1.5 hrs Lecture Ligand Docking and Designing Mutants 1.5 hrs GTPases as Biological Switches (BIOC ) Lecture Heterotrimeric G proteins I 1.5 hrs Lecture Heterotrimeric G proteins II 1.5 hrs Molecular Structure and Dynamics: Enzyme Catalysis and Modulation (BIOC 6321) Lecture Protein Kinases 1.5 hrs Lecture Protein Phosphatases 1.5 hrs 5

6 Fall Semester, 2011 Physical Biochemistry of Macromolecules (BIOC 6214) Lecture Binding Affinity and Cooperativity 1.5 hrs Lecture Rates of Binding 1.5 hrs Lecture Transition State Theory and Reaction Mechanisms 1.5 hrs Classroom Student Presentations 5 hrs Spring Semester, 2011 Molecular Structure and Graphics in the Biomedical Sciences (BIOC 6321) Lecture Structure Superpositions 1.5 hrs Lecture Ligand Docking and Designing Mutants 1.5 hrs College Spring Semester, 2013 Medical School Metabolism and Nutrition (INDT 8148) Lecture Nucleotide Metabolism I 1 hr Lecture Nucleotide Metabolism II 1 hr Review ARS review 0.5 hr Fall Semester, 2012 GPiBS Molecular and Cellular Systems I Lecture Membrane Structure: Lipids and Membrane Proteins I 1 hr Lecture Membrane Structure: Lipids and Membrane Proteins II 1 hr Spring Semester, 2012 Medical School Metabolism and Nutrition (INDT 8148) Lecture Nucleotide Metabolism I 1 hr Lecture Nucleotide Metabolism II 1 hr Review ARS review 0.5 hr Fall Semester, 2011 GPiBS Molecular and Cellular Systems I Lecture Membrane Structure: Lipids and Membrane Proteins I 1 hr Lecture Membrane Structure: Lipids and Membrane Proteins II 1 hr Campus Fall Semester, 2011 OU Norman Chemistry and Biochemistry Senior Undergraduate Capstone on Drug Design and Development (CHEM 4933) 6

7 Instructor Lecture G Protein-Coupled Receptors 1 hr Mentoring and Advising OUHSC Graduate Student General Exam Committees Jami Gurley (2013) [question mentor] Xiaolei Liu (2012) Jolyn Fernandes (2011) Chris Schafer (2011) Graduate Student Dissertation Committees Chris Schafer (2012- ) Colin Knapp (2013- ) Lee Bockus (2013- ) GPiBS Rotation Students Robert Jackson Rotation 1, Fall 2011 Jami Gurley Rotation 3, Spring 2012 Nyssa Cullin Rotation 1, Fall 2012 SURE summer undergraduate research program Sarah Colijn (2012) NARCH summer undergraduate research program Stephanie Cummings (2012) Van Andel Research Institute ( ) Mentored Kuntal Pal, a graduate student from the Department of Biological Sciences, National University of Singapore who spent one and a half years at the Van Andel Research Institute to conduct his dissertation research. His research led to a first author publication in JBC and allowed him to graduate with his Ph.D. PROFESSIONAL SERVICE Student Admissions and Recruitment Committee (2011- ) Graduate Program and Curriculum Committee (2011- ) Advisory Committee (2012- ) Space Committee (2012- ) Ad hoc for Graduate Student Gray Award Committee (2011) Ad hoc for Graduate Student Travel Award Committee (2011) Ad hoc for Graduate Student Travel Award Committee (2012) Ad hoc for Graduate Student Gray Award Committee (2013) College of Medicine COMAA seed grant review panel May

8 Campus and University OU Cancer Center Retreat Graduate Student Poster Judging Committee (2011) Journal Reviewer Structure Hormone and Metabolic Research Protein Expression and Purification August 2011 (1 review) January 2012 (1 review) June 2012 (1 review) HONORS and AWARDS Scholarship to attend the Keystone GPCR Symposium in Killarney, Ireland, May 2008 NIH genetics training grant, MEMBERSHIPS American Society for Biochemistry and Molecular Biology (ASBMB) January Biophysical Society January PROFESSIONAL GROWTH and DEVELOPMENT OUHSC Refresher on Lecturing Workshop led by Dr. Chris Candler July/August 2011 Updated 02/13/2013 8