clrphlex Chatswrth, CA Anti-breakage Benefits f Hair Care Frmulatins Objective T evaluate the anti-breakage prperties in the dry state fr tresses treated with varius test prducts. Prducts The fllwing prducts were prvided by the requester fr use in the anti-breakage study: Clr additive - Test prduct #1 (Lt# BL-013015A) Finishing Cnditiner -Test prduct #2 (BL-012915B) Experimental Hair The hair tresses weighed apprximately 3 g and measured 8 in length and 1 in width. Prir t testing, the tresses were bleached fr Cells 2, 3, & 4. Treatment All prduct treatments were in accrdance with infrmatin prvided by the requestr. Grups f tresses were treated with ne f the regimens utlined belw. All the swatches were treated as per standard prduct applicatin Cell 1 (Virgin Hair) Washed with 0.3 ml SLES/tress (massaged and rinsed fr 30 secnds) Cell 2 (9% Bleached Hair) After bleaching, tresses were standardized by washing with 0.3 ml SLES/tress (massaged and rinsed fr 30 secnds) Cell 3 (9% Bleached Hair + Clr additive) Clr additive (3.75 ml/unce f bleach) was added t the bleach mixture then standardized with 0.3 ml SLES/tress (massaged and rinsed fr 30 secnds) Cell 4 (9% Bleached Hair + Clr additive + Finishing Cnditiner) Clr additive (3.75 ml/unce f bleach) added t the bleach mixture then standardized with 0.3 ml SLES/tress (massaged and rinsed fr 30 secnds) treated with finishing cnditiner (15% -0.45ml/tress) (leave n fr 10 minutes and rinsed fr 30 secnds)
Anti-Breakage by Repeated Grming A useful methd t determine whether a treatment will prtect hair against breakage is prvided by repeated grming experiments; in this test, the number f brken fibers is recrded as a functin f repeated cmbing/brushing strkes. Any treatment that reduces snags, entanglements and abrasin can help in substantially lwering the number f brken fibers. A custm-built autmated grming device was used cnsisting f a hllw rtating drum-like assembly, where fur uter crssbars cntain hlders fr munting cmbs r brushes. These uter arms are detachable t allw fr different hlders t be munted and experiments t be perfrmed using a variety f cmbs r brushes. One cmplete drum revlutin cmbs (r brush) a tress fur times. This entire set-up is duplicated three times in the hrizntal directin allwing fur tresses t be cmbed simultaneusly. Cllectin plates are lcated under each tress t save brken fiber fragments, while spacer plates n the rtating drum prevent crss cntaminatin. All experiments were again perfrmed after vernight equilibratin f the hair at 60% RH. were repeatedly brushed 10,000 times with brken fibers being evaluated every 1000 strkes. Eight replicate hair tresses were used fr each sample t ensure statistical relevance. This methdlgy has als been dcumented in the scientific literature (Evans & Park, A Statistical Analysis f Hair Breakage. II. Repeated Grming Experiments, J.Csmet.Sci., 61, 439-455, 2010).
Repeated Grming Results Bx and whisker plt was generated using Statistica, while JMP analytical sftware was used t calculate the statistics (student s t-test at 95% cnfidence level). Raw data is given in the Appendix at the end f this reprt. Result frm repeated grming testing after 10,000 strkes is shwn belw. 140 Bx Plt f Number f brken f ibers af ter 10,000 strkes gruped by Treatment 15124 - Earthly Bdy Anti-Breakage Number f brken fibers after 10,000 strkes 120 100 80 60 40 20 0 Cell 2 Cell 3 Cell 1 Cell 4 Treatment Mean Mean±SE Mean±SD Outliers Extremes Results fr Anti-Breakage Number Treatment Mean Std Dev Std Err Mean Cell 2 (9% Bleached hair) 8 101.00 16.43 5.81 A Cell 3 (9% bleached hair + Clr Additive) 8 35.88 4.05 1.43 B Cell 1 (Virgin Hair) 8 16.88 2.80 0.99 C Cell 4 (9% bleached hair + Clr Additive + Finishing cnditiner) 8 14.00 4.44 1.57 C Levels nt cnnected by same letter are significantly different.
% Reductin in breakage is calculated as belw, Mean# Brken Fibers f Treatment % Reductin in Breakage = (1 ) 100 Mean# Brken Fibers f Cntrl Results shw that Cell 2 (9% bleached hair) presents the highest level f breakage as cmpared t the ther cells. Cell 3 (9% bleached hair + Clr Additive) shws a reductin in breakage statistically different frm Cell 2 (9% bleached hair). Ntably, Cell 4 (9% bleached hair + Clr Additive + Finishing cnditiner) shws the highest reductin in breakage ranking statistically equivalent t Cell 1 (Virgin hair).
Raw Data Brush Strkes Cell 1 (Virgin Hair) 1000 8 8 8 9 6 5 2 3 2000 3 3 2 2 2 2 3 3 3000 2 2 2 2 2 2 2 2 4000 1 1 1 1 2 2 1 1 5000 0 0 0 1 2 1 1 1 6000 2 0 0 0 1 2 1 1 7000 2 3 2 2 2 2 2 2 8000 1 1 2 1 0 0 0 0 9000 0 0 1 0 1 0 0 0 10000 0 1 1 0 0 1 0 0 Ttal 19 19 19 18 18 17 12 13 Brush Strkes Cell 2 (9% Bleached Hair) 1000 20 25 20 18 20 25 30 35 2000 8 19 10 10 10 15 16 10 3000 6 10 10 10 7 8 8 10 4000 8 9 10 10 6 9 10 15 5000 8 7 10 10 8 11 10 12 6000 5 9 10 9 9 5 9 10 7000 5 5 9 9 8 7 9 8 8000 6 7 9 9 4 6 9 9 9000 6 6 9 8 4 6 8 8 10000 5 8 8 8 4 6 8 8 Ttal 77 105 105 101 80 98 117 125
Brush Strkes Cell 3 (9% Bleached Hair + Clr Additive) 1000 10 9 12 13 10 9 11 6 2000 5 5 4 6 5 4 3 7 3000 3 3 5 6 3 3 3 4 4000 4 5 2 2 5 3 3 3 5000 2 3 3 3 4 3 2 3 6000 1 2 2 3 3 4 2 2 7000 1 1 2 2 3 2 3 4 8000 2 1 1 1 2 4 2 2 9000 0 3 3 5 3 2 3 3 10000 2 1 1 2 2 2 2 2 Ttal 30 33 35 43 40 36 34 36 Brush Strkes Cell 4 (9% Bleached Hair +Clr Additive + Finishing Cnditiner) 1000 4 4 5 6 5 4 5 4 2000 2 2 1 1 3 2 4 4 3000 1 1 1 1 2 2 1 1 4000 1 0 1 1 2 3 0 0 5000 1 2 1 1 2 3 1 1 6000 0 1 0 0 1 0 2 2 7000 1 1 0 0 2 1 0 0 8000 0 1 1 2 2 1 1 1 9000 0 0 0 0 2 1 0 0 10000 0 0 0 0 2 1 0 0 Ttal 10 12 10 12 23 18 14 13