(Total for Question 1 = 7 marks)

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Biology B Advanced Level Paper 2 (9BI0/02) 1(a)(i) D (proteins, nucleic acids, starch) 1(a)(ii) 1(a)(iii) C (lactose) D (mrna) 1(b) (a mutation results in) a different sequence of amino acids therefore different R groups / side chains resulting in changes to { ionic / hydrogen bonding / disulphide bridges } change in shape of active site causing a change in enzyme activity (4) (Total for 1 = 7 marks) 1

2(a)(i) 2(a)(ii) 2(b) C (nucleic acid and protein) B (human immunodeficiency virus) A description that makes reference to the following: Latency: virus genetic material inserted into host cell and integrated into host DNA viral DNA replicated each time the host cell replicates Lytic cycle: virus genetic material inserted into host cell and synthesis of viral particles by host cell lysis of host cell (4) (Total for 2 = 6 marks) 2

3(a) Example of calculation overall net filtration pressure is 3.2 9.2 2.0 = 7.2 so fluid will be forced from the glomerulus into the Bowman s capsule 7.2 4.0 = 3.2 =3.2 (2) 3(b) active transport of chloride ions out of ascending limb of loop of Henle causes a decrease in water potential of the medulla ascending limb impermeable to water therefore water reabsorbed from the descending limb by osmosis (4) 3(c) D (increased secretion of ADH and increased permeability of the collecting duct) (Total for 3 = 7 marks) 3

4(a) A (fertile offspring) 4(b) An explanation that makes reference to five of the following: geographical isolation between Fuerteventura and mainland birds birds exposed to different selection pressures variation between individuals therefore increased survival / reproduction of individuals with advantageous { alleles / genes } increasing frequency of (these) alleles reproductive isolation / allopatric speciation (5) 4(c) captive-breeding programmes to increase numbers ensure outbreeding to maintain genetic diversity to re-introduce (genetically diverse) birds into a suitable habitat (3) (Total for 4 = 9 marks) 4

5(a)(i) A (P) 5(a)(ii) (no P wave on trace) sinoatrial node / SAN not functioning causing lack of electrical activity in the atria failing to pass electrical signal to the atrioventricular node (3) 5(a)(iii) normal ECG trace rate is 60 beats per minute and person with Sick Sinus Syndrome rate is 50 beats per minute so difference 10 beats per minute (2) 5(a)(iv) sinoatrial node is not working causing a slower heart beat rate causing lack of oxygen to { brain / muscles } (2) (Total for 5 = 8 marks) 5

6(a)(i) 6(a)(ii) C (Paramecium is a eukaryote and the bacterium is a prokaryote) pond water has greater water potential than the cytoplasm therefore water enters by osmosis Paramecium has { only cell membrane / has no cell wall } so would burst if water not removed (3) 6(b)(i) Allow converse statements locust has a smaller surface area to volume ratio than Paramecium therefore has { tracheae / tracheoles } to provide greater surface area (for gas exchange / diffusion) (2) 6

6(b)(ii) Indicative content Answers will be credited according to candidate's deployment of knowledge and understanding of the material in relation to the qualities and skills outlined in the generic mark scheme. The indicative content below is not prescriptive and candidates are not required to include all the material which is indicated as relevant. Additional content included in the response must be scientific and relevant. in human exhaled air abdominal pumping movements rise, but since exhaled air is warmer / more moist, contains more carbon dioxide and less oxygen than atmospheric air no conclusion can be reached from this result in the presence of pure oxygen the number of abdominal pumping movements is lower than when in atmospheric air in the presence of pure carbon dioxide the number of abdominal pumping movements is higher than when in atmospheric air in atmospheric air there is some residual effect of previous gas exposure Level Mark Descriptor 0 No awardable content 1 1-2 An explanation may be attempted but with limited interpretation or analysis of the scientific information with a focus on mainly just one piece of scientific information. The explanation will contain basic information with some attempt made to link knowledge and understanding to the given context. 2 3-4 An explanation will be given with occasional evidence of analysis, interpretation and/or evaluation of both pieces of scientific information. 3 5-6 The explanation shows some linkages and lines of scientific reasoning with some structure. An explanation is made which is supported throughout by sustained application of relevant evidence of analysis, interpretation and/or evaluation of both pieces of scientific information. The explanation shows a well-developed and sustained line of scientific reasoning which is clear and logically structured. (Total for 6 = 12 marks) 7

7(a) An explanation that makes reference to four of the following: colonisation by pioneer species Allow lichens which contribute dead organic matter to form soil therefore creating suitable conditions for other species to establish leading to { succession / a sequence of changes } in {a community / organisms / species / plants} the final { stage / sere / community } is { self-sustaining / stable } (4) 7(b)(i) Euphorbia regis-jubiae is a producer therefore provides { food / energy } for other organisms (herbivores / primary consumers / decomposers) { presence of roots / organic material } holds soil structure together / increases nutrients therefore it provides { shelter / (micro) habitat } for other organisms to establish themselves (3) 8

7(b)(ii) Accept converse statements high light intensity promotes growth due to increased photosynthesis / high temperature promotes growth due to rapid rates of reaction however lack of { rainfall / water } limits { photosynthesis / transpiration } rate however { wind / high temperature } increases { transpiration rate / water loss } however wind results in soil erosion therefore poor soil quality (4) (Total for 7 = 11 marks) 9

8(a) A genetic diagram that makes reference to the following: parent genotype HhPp x HhPp Gametes HP Hp hp hp HP HHPP HHPp HhPP HhPp Hp HHPp HHpp green HhPp Hhpp green hp HhPP HhPp hhpp smooth hhpp smooth hp HhPp Hhpp green hhpp smooth hhpp smooth green gametes formed all correct genotypes all correct phenotypes and ratios correct (4) 10

8(b)(i) An answer that makes reference to the following: Example of calculation calculation difference calculation of percentage difference = 14-5 = 9 kg (9 5) x 100 = 180 % Correct answer with no working gains full marks (2) 8(b)(ii) 8(b)(iii) there is no difference between GM and non-gm potato yields ALLOW no significant difference An answer that makes reference to the following: Example of calculation use Student s t-test to calculate the value of t (2) 2.9 2 6 = 1.4017 and 2.1 2 6 = 0.735 correct answer (1.4017 + 0.735) = 1.462 (12.0 5.0) 1.462 = t = 4.788 = 4.79 Correct answer with no working gains full marks (3) 11

8(b)(iv) An answer that makes reference to the following: Allow ECF for incorrect value of t p < 0.05 difference is not due to chance Allow 0.005 so GM potatoes have significantly greater yield than non-gm (3) (Total for 8 = 13 marks) 12

9(a) An answer that makes reference to the following: calculation of frequency of homozygous recessives f(tt) Example of calculation Allow ECF for incorrect values 100-64% = 36% = 0.36 calculation of q calculation of p calculation of f(tt) and f(tt) 0.36 q = = 0.6 p =1 0.6 = 0.4 p 2 = 0.4 2 = 0.16 2pq = 2 x 0.4 x 0.6 = 0.48 (4) 9(b)(i) A description that makes reference to any two of the following: population is large mating is random mutation is rare migration does not alter allele frequency / does not happen / the population is isolated (2) 9(b)(ii) An answer that makes reference to the following: founder effect population bottleneck genetic drift / result of chance (3) (Total for 9 = 9 marks) 13

10(a) to prevent growth of { bacteria / fungi } which would produce amylase to prevent { bacteria / fungi } from decomposing the seeds (2) 14

10(b) Indicative content Answers will be credited according to candidate's deployment of knowledge and understanding of the material in relation to the qualities and skills outlined in the generic mark scheme. The indicative content below is not prescriptive and candidates are not required to include all the material which is indicated as relevant. Additional content included in the response must be scientific and relevant. seed half with embryo does have amylase activity seed half without embryo has no amylase activity seed half without embryo and with the gibberellin does have amylase activity this suggests gibberellin is needed for amylase { synthesis / activity } selectively permeable membrane shows that the active agent / gibberellins is a small molecule amylase is not diffusing through the membrane Level Mark Descriptor 1 1-2 0 No awardable content Demonstrates isolated elements of biological knowledge and understanding to the given context with generalised comments made. The explanation will contain basic information with some attempt made to link knowledge and understanding to the given context. Demonstrates adequate knowledge and understanding by selecting and applying some relevant biological facts/concepts to provide the explanation being presented. 2 3-4 Lines of argument occasionally supported through the application of relevant evidence (scientific ideas, processes, techniques and procedures). The explanation shows some linkages and lines of reasoning with some structure. Demonstrates comprehensive knowledge and understanding by selecting and applying relevant knowledge of biological facts/concepts to provide the explanation being presented. 3 5-6 Line(s) of argument supported throughout by sustained application of relevant evidence (scientific ideas, processes, techniques and procedures). The explanation shows a well-developed and sustained line of reasoning which is clear, coherent and logically structured. (Total for 10 = 8 marks) 15