Optimisil HPLC & UPLC Column catalogue

Optimisil HPLC & UPLC Column catalogue Analytical Hub Plot No: 37, P & T Colony, Vikrampuri, Secunderabad 500009 Tel: 040-64621313, Mobile: +91 7799931313 Website: www.analyticalhub.com email: contact@analyticalhub.com

Contents C18 Reverse phase HPLC columns MF C18 AQ C18 HL C18 LP C18 MC C18 Other Reverse phase HPLC columns MF C8 LP C8 MF C4 LP C4 MF phenyl PolyRP (Polymer based) Normal phase HP Cyano AQ Silica HP Amino Preparation and Semi preparation columns polar compound small molecule acid and basic compound peptide 200 protein 300 hydrophilic

Special HPLC columns UHPLC 1.8 μm 2.2 μm Nano and capillary column C18

Optimisil C18 Reversed Phase LC Columns Characteristics Highly controlled chemistry of monolayer formation and end capping Extremely high column to column reproducibility High selectivity and efficiency MF C18 AQ C18 HL C18 LP C18 MF C18 uses full coverage bonded silica packing, which provides exceptionally high stability. The unique mono functional bonding chemistry of MF C18 avoids the formation of multiple C18 layers. Such uniform stationary phase allows the separation to achieve high selectivity and high efficiency. AQ C18 uses full coverage bonded silica packing, which provides exceptionally high stability. Compatible with 100% aqueous mobile phase suitable for separations of acidic, neutral and basic organic compounds, as well as pharmaceuticals, peptides, and others. HL C18 uses a proprietary ODS bonding chemistry to a high surface area silica gel to achieve long retention time and high loading capability. The ODS density is specially designed to achieve optimal selectivity for both hydrophobic and hydrophilic compounds. The proprietary end capping chemistry maximizes consumption of silanol groups on the silica surface,and minimizes the free silanol groups to an undetectable leve. Such unique bonding chemistry allows the phase to achieve high peak symmetry even for basic compounds. HL C18 phase is compatible with 100% aqueous mobile phases. The uniform stationary phase allows the separation to achieve high selectivity and high efficiency. Pore size selection of 200 and 300Å and high compatibility with 100% aqueous phases make LP C18 columns ideal for high resolution mapping of peptides and separation of

natural and synthetic peptides and small proteins. The specially designed surface bonding chemistry and the pore sizes allow for extended retention and selectivity for polar and hydrophilic compound, such as peptides and amino acids. MC C18 Utilizing highest purity and enhanced mechanical stability silica and pure bonding reagents, MC C18 bonded phases have been innovatively and specially designed to ensure maximum surface coverage and full end capping, which leads to carbon content as high as 19.0%. The bonding chemistry is completely controlled that results in very reliable column to column reproducibility. The maximum surtace coverage allows MC C18 to have exceptional stability, resulting in high ph stability in the range of1.5 to 10.5. Specifications MF C18 AQ C18 HL C18 LP C18 MC C18 Silica Pore size: 120 Å Spherical, high purtity (<10ppm metals) 120 Å 200 Å 100 Å 200 Å 300 Å 120 Å Pore volume: 1.0 Ml/g 1.0 Ml/g 1.0 Ml/g 1.1Ml/g 1.0 Ml/g 0.95Ml/g 1.0 Ml/g Surface area: 300 m2 /g 300 m2 /g 200 m2 /g 450 m2 /g 200 m2 /g 105 m2 /g 350 m2 /g Phase Special bonding structure: Monomeric and fully end capped density and proprietary Monomeric and fully end capped Fully end capped end capping % Carbon: 17 % 17 % 10% 17 % 10% 7 % 19.0 %

MF C18 Specification Silica: Spherical, high purity (<10 ppm metals) Pore size: 120 Å Pore volume: 1.0 ml/g Surface area: 300 m 2 /g Phase structure: Monomeric and fully endcapped % Carbon: 17% Characteristics Highly controlled chemistry of monolayer formation and end capping Extremely high column to column reproducibility High selectivity and efficiency for separations Suitable for separations of acidic, neutral and basic organic compounds as well as pharmaceutical, peptides, and others Recommended for separations in organic or mixed organic/aqueous mobile phases Description MF C18 uses full coverage bonded silica packing, which provides exceptionally high stability. Figure 1 shows the highly reproducible retention time for three standard compounds: aniline, anisole and toluene after 13,000 column volume runs in a mobile phase of 85% methanol and 15% water. Such high stability allows MF C18 extremely suitable for validation of various analytes.

Fig: MF C18 (4.6x150 mm) Stability Test The unique mono functional bonding chemistry of MF C18 avoids the formation of multiple C18 layers. Such uniform stationary phase allows the separation to achieve high selectivity and high efficiency. A typical test chromatogram for quality control is shown in Figure 2 using a 4.6x250mm MF C18 column.

Applications Organic acid Column: MF C18, 4.6x150mm, 5 μm Eluent: 0.10 M Phosphate buffer, ph 3.1 Flow rate: 1.0 ml/min Detection: UV 210 nm Injection: 5 μl Temperature: Ambient (23 C) Compounds: Organic Acids (10 mm) Alkaloids Alkaloids are naturally occurring basic compounds with heterocyclic ring structures. Due to ion exchange and electrostatic interaction with the residual silanol(si OH), the separations with silica based reverse phases such as C18 packing, usually result in poor peak shape. This unique bonding chemistry of MF C18 columns enables separation of alkaloids with high selectivity and high resolution

Column: MF C18, 4.6 mm x 150 mm, 5 μm Mobile phase: 0.1 M phosphate buffer, ph3.1 Flow rate: 0.75 ml/min Detection :UV 254 nm Injection: 5 μl Temperature: 23 Samples: 1.Theobromine(1 mm), 2. Theophyline(1 mm)3. Caffeine(1 mm) 4.Phenol(7 mm) Catecholamines Column: MF C18, 4.6x150mm, 5 μm Eluent: 57 mm Citric Acid/43 mm NaAc/0.10 mm EDTA (disodium form) / 1 mm Octanesulfonic Acid /10% MeOH (ph 3.4) Flow rate: 1.0 ml/min Detection: Electrochemical Detection with GC (+0.70 V vs. Ag/AgCl, 3 M KCl) Injection: 25 μl Temperature: Ambient (23 C) min Compounds: 1. Norepinephrine (100 nm) 2. Ephinephrine (100 nm) 3. Dihydroxybenzylamine (100 nm) 4. Dopamine (100 nm)

HL C18 Specification Silica: Spherical, high purity (<10 ppm metals) Pore size: 100 Å Particle size: 3, 5 and 10 μm Pore volume: 1.1 ml/g Surface area: 450 m 2 /g Phase structure: Special bonding density and proprietary endcapping % Carbon: 17% Description HL C18 uses a proprietary ODS bonding chemistry to a high surface area silica gel to achieve long retention time and high loading capability. The ODS density is specially designed to achieve optimal selectivity for both hydrophobic and hydrophilic compounds. The proprietary endcapping chemistry maximizes consumption of silanol groups on the silica surface, and minimizes the free silanol groups to undetectable level. Such unique bonding chemistry allows the phase to achieve high peak symmetry, even for basic compounds. HL C18 phase is compatible with 100% aqueous mobile phases. A test chromatogram using Standard Reference Material 870 is shown in Figure 1.

Column: HL C18 (4.6 150mm, 5 μm) Mobile phase: MeOH:20mM potassium phosphate (ph 7.0) = 80:20 (v/v) Flow rate: 1.0 ml/min Detection: 210 nm Temperature: 23 oc Sample: 1. uracil 2. toluene 3. ethylbenzene 4. quinizarin 5.amitriptyline Figure 1. Standard Reference Material 870 compounds are separated by a HL C18 column. Ethyl benzene: USP plate counts, 13,500; symmetry, 0.96. Characteristics Specially designed ODS density bonded to highly pure silica Free silanol groups on silica surface is minimized to undetectable level High load ability Compatible with 100% aqueous mobile phases High column to column reproducibility Optimal selectivity for both hydrophobic and hydrophilic compounds Available for both analytical and preparative

LP C18 C18 monolayer formed by special bonding chemistry does not collapse in pure aqueous solution. Specification Silica: Spherical, high purity (<10 ppm metals) Pore size: 200 Å Particle size: 3, 4, 5 and 10 μm Pore volume: 1.0 ml/g Surface area: 200 m2/g Phase structure: Monomeric and fully endcapped % Carbon: 10% Pore size: 300 Å Particle size: 3, 5 μm Pore volume: 0.95 ml/g Surface area: 105 m2/g Phase structure: Monomeric and fully endcapped % Carbon: 7.0% Description Wide pore size and high compatibility with 100% aqueous phases make LP C18 columns ideal for high resolution mapping of peptides and separation of natural and synthetic peptides and small proteins. The specially designed surface bonding chemistry and the pore sizes allow for extended retention and selectivity for polar and hydrophilic compounds, such as peptides and amino acids. Characteristics Highly controlled chemistry of monolayer formation and end capping Extremely high column to column reproducibility High selectivity and efficiency for separations Compatible with 100% aqueous mobile phase Suitable for separations of peptides, proteins, and pharmaceuticals

Applications Separation of Peptides Column: LP C18 (5 μm, 200 Å) 4.6x250 mm Mobile phase: (A) 5% ACN, 0.1% TFA in H2O (B) 50% ACN, 0.1% TFA in H2O Isocratic: 65% A, 35% B Flow rate: 1.0 ml/min Detection (UV): 214 nm Injection Volume: 5 μl Peptides: 1. VTSRGNVGGG 2. QITLPNHGGG 2a/2b: two impurities from peptide 2 Figure.Separation of peptides and impurities.

MC C18 C18 phase formed by special bonding chemistry for applications in wide range of ph (1.5 10.5) Specification Silica: Spherical, high purity (<10 ppm metals) Pore size: 120 Å Particle size: 3, 5 μm Pore volume: 1.0 ml/g Surface area: 350 m 2 /g Phase structure: Monomeric and fully endcapped % Carbon: 19.0% Characteristics Highly controlled chemistry of monolayer formation and end capping Extremely high column to column reproducibility High selectivity and efficiency for separations ph range 1.5 10.5 Suitable for separations of acidic, neutral and basic Description Utilizing highest purity and enhanced mechanical stability silica and pure bonding reagents, MC C18 bonded phases have been innovatively and specially designed to ensure maximum surface coverage and full end capping, which leads to carbon content as high as 19.0%. The bonding chemistry is completely controlled that results in very reliable column to column reproducibility.the maximum surface coverage allows MC C18 to have exceptional stability, resulting in high ph stability in the range of 1.5 to 10.5.

Column Stability at Alkali Conditions MC C18 uses full coverage bonded silica packing, which allows high stability at high ph. Figure 1 shows reproducible retention time for a test compound: toluene after 18,000 column volume runs in a mobile phase of 55% acetonitrile and 45% water at ph 10. Such high stability allows BR C18 well suited for validation of various analytes at alkali conditions. The proprietary bonding chemistry for BR C18 allows achieving high selectivity and high efficiency separation. Figure 1. A MC C18 column (3 µm, 4.6x50 mm) was operated at ph 10 under the conditions: mobile phase, 10 mm ammonium bicarbonate buffer in 55%ACN/45%H2O; flow rate, 0.5 ml/min; room temperature; detection, UV 254 nm; sample, toluene.

Optimisil Reverse phase C18 column list ID*Length (mm) Particle Size(um) MF C18 (120Å) AQ C18 (120Å) 2.0x10(guard column) 3 SP 03020010 01120C SP 03020010 01120C SP 03020010 05200C SP 03020010 05300C SP 03020010 04120C 2.1x30 3 SP 03021030 01120 SP 03021030 01120 SP 03021030 05200 SP 03021030 05300 SP 03021030 04120 2.1x50 3 SP 03021050 01120 SP 03021050 01120 SP 03021050 03100 SP 03021050 05200 SP 03021050 05300 SP 03021050 04120 4.0x10.(guard column) 3 SP 03040010 01120C SP 03040010 01120C SP 03040010 05200C SP 03040010 05300C SP 03040010 04120C 4.6x100 3 SP 03046100 01120 SP 03046100 01120 SP 03046100 05200 SP 03046100 05300 SP 03046100 04120 4.6x150 3 SP 03046150 01120 SP 03046150 01120 SP 03046150 03100 SP 03046150 05200 SP 03046150 05300 SP 03046150 04120 4.6x250 3 SP 03046250 01120 SP 03046250 01120 SP 03046250 03100 SP 03046250 05200 SP 03046250 05300 SP 03046250 04120 2.0x10.(guard column) 5 SP 05020010 01120C SP 05020010 01120C SP 05020010 05200C SP 05020010 05300C SP 05020010 04120C 2.1x30 5 SP 05021030 01120 SP 05021030 01120 SP 05021030 05200 SP 05021030 05300 SP 05021030 04120 2.1x50 5 SP 05021050 01120 SP 05021050 01120 SP 05021050 03100 SP 05021050 05200 SP 05021050 05300 SP 05021050 04120 4.0x10.(guard column) 5 SP 05040010 01120C SP 05040010 01120C SP 05040010 05200C SP 05040010 05300C SP 05040010 04120C 4.6x100 5 SP 05046100 01120 SP 05046100 01120 SP 05046100 05200 SP 05046100 05300 SP 05046100 04120 4.6x150 5 SP 05046150 01120 SP 05046150 01120 SP 05046150 03100 SP 05046150 05200 SP 05046150 05300 SP 05046150 04120 4.6x250 5 SP 05046250 01120 SP 05046250 01120 SP 05046250 03100 SP 05046250 05200 SP 05046250 05300 SP 05046250 04120 HL C18 (100Å) LP C18 (200Å) LP C18 (300Å) MC C18 (120Å)

Optimisil Other Reversed Phase HPLC Columns Characteristics Highly controlled chemistry of monolayer formation and end capping Extremely high column to column reproducibility High selectivity and efficiency for separations Full coverage bonded silica packing to achieve the exceptionally high stability MF C8 phase is synthesized with monomeric and fully endcapped chemistry. The uniform octyl stationary phase allows high efficiency with lower hydrophobicity compared to ODS phase MF C8 phase is MF C8 suitable for separating compounds with a wide range of hydrophobicity. It is highly recommended for separating the compounds which are too strongly retained on C18 phases. LP C8 phase is made of monomeric and fully endcapped chemistry. The uniform stationary phase allows the separation to achieve high selectivity and high efficiency. LP C8 packings of 300 A pore size are LP C8 ideal for high resolution Mapping of peptides and separation of natural and synthetic peptides and small proteins. Monomeric and fully endcapped MF packing is bonded with butyl group that leads to moderate hydrophobicity. MF C4 columns have MF C4 the great selectivity and peak symmetry with moderate retention for separations of acidic, neutral and basic organic compounds, such as pharmaceuticals, peptides and organic acids. Monomeric and fully endcapped MF C4 packing is bonded with butyl group that leads to moderate hydrophobicity. LP C4 packings LP C4 of 300A pore size are ideal for high resolution mapping of peptides and separation of natural and synthetic peptides and small proteins. MF Phenyl packing materials are bonded with propyl phenyl groups that enable special interaction with ring structured compounds. The monomeric bonding chemistry gives very high efficiency and high MF Phenyl resolution separations. MF Phenyl phase is suitable for separations of acidic, neutral and basic organic compounds, as well as the pharmaceuticals. Specifications MF C8 MF C4 MF Phenyl LP C8 LP C4 Silica Spherical, high purtity (<10 ppm metals) Pore size: 120 A 300A 300A Particle size: 3, 4, 5, 7 and 10 μm 3.5 and 10 μm Pore volume: 1.0 ml/g 0.9 ml/g 1.0 ml/g Surface area: 300 m2 /g 105 m2 /g Phase structure: Monomeric and fully end capped % Carbon: 11% 8.0% 11% 4.0% 3.0% Coverage: 3.0 umol/ m2

Optimisil Other reverse phase column ID*Length (mm) Particle Size(um) MF C8 LP C8 MF C4 LP C4 MF Phenyl 2.0x10(guard column) 3 SP 03020010 06120C SP 03020010 09300C SP 03020010 07120C SP 03020010 08300C SP 03020010 10120C 2.1x30 3 SP 03021030 06120 SP 03021030 09300 SP 03021030 07120 SP 03021030 08300 SP 03021030 10120 2.1x50 3 SP 03021050 06120 SP 03021050 09300 SP 03021050 07120 SP 03021050 08300 SP 03021050 10120 4.0x10.(guard column) 3 SP 03040010 06120C SP 03040010 09300C SP 03040010 07120C SP 03040010 08300C SP 03040010 10120C 4.6x100 3 SP 03046100 06120 SP 03046100 09300 SP 03046100 07120 SP 03046100 08300 SP 03046100 10120 4.6x150 3 SP 03046150 06120 SP 03046150 09300 SP 03046150 07120 SP 03046150 08300 SP 03046150 10120 4.6x250 3 SP 03046250 06120 SP 03046250 09300 SP 03046250 07120 SP 03046250 08300 SP 03046250 10120 2.0x10(guard column) 5 SP 05020010 06120C SP 05020010 09300C SP 05020010 07120C SP 05020010 08300C SP 05020010 10120C 2.1x30 5 SP 05021030 06120 SP 05021030 09300 SP 05021030 07120 SP 05021030 08300 SP 05021030 10120 2.1x50 5 SP 05021050 06120 SP 05021050 09300 SP 05021050 07120 SP 05021050 08300 SP 05021050 10120 4.0x10.(guard column) 5 SP 05040010 06120C SP 05040010 09300C SP 05040010 07120C SP 05040010 08300C SP 05040010 10120C 4.6x100 5 SP 05046100 06120 SP 05046100 09300 SP 05046100 07120 SP 05046100 08300 SP 05046100 10120 4.6x150 5 SP 05046150 06120 SP 05046150 09300 SP 05046150 07120 SP 05046150 08300 SP 05046150 10120 4.6x250 5 SP 05046250 06120 SP 05046250 09300 SP 05046250 07120 SP 05046250 08300 SP 05046250 10120 10.0x150 5 SP 05010150 01120 SP 05010150 01300 SP 05010150 01120 SP 05010150 01300 SP 05010150 01120 10.0x250 5 SP 05010250 01120 SP 05010250 01300 SP 05010250 01120 SP 05010250 01300 SP 05010250 01120 21.2x10.(guard column) 5 SP 05212010 01120C SP 05212010 01300C SP 05212010 01120C SP 05212010 01300C SP 05212010 01120C 21.2x150 5 SP 05212150 01120 SP 05212150 01300 SP 05212150 01120 SP 05212150 01300 SP 05212150 01120 21.2x250 5 SP 05212250 01120 SP 05212250 01300 SP 05212250 01120 SP 05212250 01300 SP 05212250 01120 30.0x150 5 SP 05030150 01120 SP 05030150 01300 SP 05030150 01120 SP 05030150 01300 SP 05030150 01120

30.0x250 5 SP 05030250 01120 SP 05030250 01300 SP 05030250 01120 SP 05030250 01300 SP 05030250 01120 10.0x150 10 SP 10010150 01120 SP 10010150 01300 SP 10010150 01120 SP 10010150 01300 SP 10010150 01120 10.0x250 10 SP 10010250 01120 SP 10010250 01300 SP 10010250 01120 SP 10010250 01300 SP 10010250 01120 21.2x150 10 SP 10212150 01120 SP 10212150 01300 SP 10212150 01120 SP 10212150 01300 SP 10212150 01120 21.2x250 10 SP 10212250 01120 SP 10212250 01300 SP 10212250 01120 SP 10212250 01300 SP 10212250 01120 30.0x150 10 SP 10030150 01120 SP 10030150 01300 SP 10030150 01120 SP 10030150 01300 SP 10030150 01120 30.0x250 10 SP 10030250 01120 SP 10030250 01300 SP 10030250 01120 SP 10030250 01300 SP 10030250 01120

Optimisil Poly RP

Poly RP Specifications Porous Packings PS/DVB Particles: spherical, 80% cross linking Pore size: 100, 300, 500 and 1000 Å Particle size: 5 and 10 μm Pore volume: 1.0 ml/g Surface area: 280 m 2 /g for 100 Å pore size Phase structure: phenyl group Separation mechanism: hydrophobic interaction Non Porous Resins PS/DVB Particles: spherical, 80% cross linking Pore size: non porous Particle size: 3, 5 and 10 μm Surface area: <10 m 2 /g Phase structure: phenyl group Separation mechanism: hydrophobic interaction 1 Description PolyRP phases are made of 80% cross linking PS/DVB spherical particles. Those highly rigid particles have both non porous and porous structures with the particle size selection of 3, 5, and 10 μm. The phase structure is phenyl functional group that enables hydrophobic interaction. Compared with silica based reversed phases, PolyRP phases have advantages over applications at extreme ph (1 14) with special selectivity and slightly lower separation efficiency. Characteristics Uniform particles and narrow pore size distribution Extremely high chemical stability Unsurpassed ph stability (1 14) High retentativity Better selectivity High mechanical stability

Optimisil Normal Phase LC Columns Characteristics Highly controlled chemistry of monolayer formation and end capping Extremely high column to column reproducibility High selectivity and efficiency for separations AQ Cyano Cyanide acrylate based Synthesized with monomeric and fully endcapped chemistry, AQ Cyano phase is bonded with propylcyano functional group that allows special Interaction with polar compounds. The monomeric bonding chemistry enables high efficiency and high resolution separation of peptide, proteins, acidic, neutral and basic organic compound, and pharmaceuticals. AQ Amino Ammonia based Synthesized with polymeric chemistry, AQ Amino phase is bonded with aminopropyl functional group. HP Amino phase is compatible with versatile mobile phases from non aqueous solvents, such as hexane/ethyl acetate and chloroform/methanol, to aqueous solutions. It is recommended for separations of sugars, nucleotides, basic organic compounds as well as the pharmaceuticals. AQ Diol Synthesized with polymeric chemistry, AQ Diol phase is bonded with 1,2 dihydroxypropyl propyl ether 1,2 dihydroxy propyl ether functional group that allows special interaction with based polar compounds. The polymeric bonding chemistry enables high efficiency and high stability separation of polar pharmaceuticals, peptide, and proteins. AQ Diol phase can also be used as size exclusion separation of biological molecules. AQ Silica Silica based AQ Silica phase is made of activated hydroxyl ( OH) functional group with the pore size selection of 60, 120, 200, 300, 500, 1000 and 2000 A and particles size selection of 1.8, 2.2, 3, 5 and 10 um Carbon loading is 0.0%. AQ Silica is used as the normal phase as well as HILIC phase. AQ Silica phases are suitable for separations of polar and basic organic compounds, such as vitamins, steroids as well as pharmaceuticals. Specifications AQ Cyano AQ Amino AQ Diol AQ Silica Silica Spherical, high purtity (<10ppm metals) Pore size: 120 A 80 and 120 A 120, 200, 300, 500, 1000, and 2000 A Particle size: 3, 4, 5, 7 and 10μm 3.5 and 10 μm 3, 4, 5, 7 and 10 μm Pore volume: 1.0 ml/g 1.0 ml/g (120 A pore size) Surface area: 300 m2 /g 300 m2 /g 300 m2 /g 300 m2 /g (120 A pore size) Phase structure: Monomeric and fully end capped Activated hydroxyl ( OH) % Carbon: 7.0% 4.0% 8.8% Coverage: 3.5 umol/ m2

Optimisil Semi preparation and preparation column CVC pore size Å Surface area ligand type phase available pore size silica type end cap carbon load small molecules 100 190 C8, C18, Silica 5 um A fully endcapped C8:7 % C18:12 % Acid/Basic compounds 100 430 monofunctional C18 3,4.5,10 polar compound 120 monofunctional C8,C18, Silica,Cy ano,phe nyl B(ultra pure) proprietary and extremely exhaustive(fully capping) 18% 4.5,10 B Extremely well 18% Peptide (19 KDa/less) 200 200 monofunctional C4,18 4.5,10 B fully endcapped C4:4.5 % C18: 14 % Protein 300 100 monofunctional C4,C18 4.5, 10 B fully endcapped C4:3 % C18: 8 % hydrophilic 120 330 monofunctional C8,C18 3,4,5,10 B unique polar and bulky end capping 18%

Optimisil Polar Compound(PCHGCS) 10 mm Semi Prep and 20 mm Prep Columns Polar Compound(PCHGCS) C8 5μm 120A 10 and 20mm I.D. s.s. columns 50x10mm 100x10mm 50x10mm 100x10mm HG05100050 09120 HG05100100 09120 HG05100050 09120 HG05100100 09120 50x20mm 100x20mm 50x20mm 100x20mm HG05200050 09120 HG05200100 09120 HG05200050 09120 HG05200100 09120 Polar Compound(PCHGCS) C18 5μm 120A 10 and 20mm I.D. s.s. columns 50x10mm 100x10mm 50x10mm 100x10mm HG05100050 03120 HG05100100 03120 HG05100050 03120 HG05100100 03120 50x20mm 100x20mm 50x20mm 100x20mm HG05200050 03120 HG05200100 03120 HG05200050 03120 HG05200100 03120 Polar Compound(PCHGCS) Silica 5μm 120A 10 and 20mm I.D. s.s. columns 50x10mm 100x10mm 50x10mm 100x10mm HG05100050 14120 HG05100100 14120 HG05100050 14120 HG05100100 14120 50x20mm 100x20mm 50x20mm 100x20mm HG05200050 14120 HG05200050 14120 HG05200050 14120 HG05200050 14120 Polar Compound(PCHGCS) C18 10μm 120A 10 and 20mm I.D. s.s. columns 50x10mm 100x10mm 50x10mm 100x10mm HG10100050 03120 HG10100100 03120 HG10100050 03120 HG10100100 03120 50x20mm 100x20mm 50x20mm 100x20mm HG10200050 03120 HG10200100 03120 HG10200050 03120 HG10200100 03120 Polar Compound(PCHGCS) Silica 10μm 120A 10 and 20mm I.D. s.s. columns 50x10mm 100x10mm 50x10mm 100x10mm HG10100050 14120 HG10100100 14120 HG10100050 14120 HG10100100 14120 50x20mm 100x20mm 50x20mm 100x20mm HG10200050 14120 HG10200100 14120 HG10200050 14120 HG10200100 14120

Small Molecules( SMHHA100) 10mm Semi Prep and 20mm Prep Columns Small Molecules(SMHHA100) C8 5μm 100A 10 and 20mm I.D. s.s. columns 50x10mm 100x10mm 50x10mm 100x10mm HG05100050 08100 HG05100100 08100 HG05100050 08100 HG05100100 08100 50x20mm 100x20mm 50x20mm 100x20mm HG05200050 08100 HG05200100 08100 HG05200050 08100 HG05200100 08100 Small Molecules(SMHHA100) C18 5μm 100A 10 and 20mm I.D. s.s. columns 50x10mm 100x10mm 50x10mm 100x10mm HG05100050 01100 HG05100100 01100 HG05100050 01100 HG05100100 01100 50x20mm 100x20mm 50x20mm 100x20mm HG05200050 01100 HG05200100 01100 HG05200050 01100 HG05200100 01100 Acid & Basic (ABGHPX) 10mm Semi Prep and 20mm Prep Columns Acid & Basic(ABGHPX) C18 10μm 100A 10 and 20mm I.D. s.s. columns 50x10mm 100x10mm 50x10mm 100x10mm HG10100050 02100 HG10100100 0100 HG10100050 02100 HG10100100 0100 50x20mm 100x20mm 50x20mm 100x20mm HG10200050 02100 HG10200100 0100 HG10200050 02100 HG10200100 0100 Acid & Basic(ABGHPX) C18 5μm 100A 10 and 20mm I.D. s.s. columns 50x10mm 100x10mm 50x10mm 100x10mm HG05100050 02100 HG05100100 02100 HG05100050 02100 HG05100100 02100 50x20mm 100x20mm 50x20mm 100x20mm HG05200050 02100 HG05200100 02100 HG05200050 02100 HG05200100 02100 PEPTIDE200 10mm Semi Prep and 20mm Prep Columns Peptide(PEPTIDE200) C4 10μm 200A 10 and 20mm I.D. s.s. columns 50x10mm 100x10mm 50x10mm 100x10mm HG10100050 15200 HG10100100 15200 HG10100050 15200 HG10100100 15200 50x20mm 100x20mm 50x20mm 100x20mm HG10200050 15200 HG10200100 15200 HG10200050 15200 HG10200100 15200 Peptide(PEPTIDE200) C18 10μm 200A 10 and 20mm I.D. s.s. columns

50x10mm 100x10mm 50x10mm 100x10mm HG10100050 04200 HG10100100 04200 HG10100050 04200 HG10100100 04200 50x20mm 100x20mm 50x20mm 100x20mm HG10200050 04200 HG10200100 04200 HG10200050 04200 HG10200100 04200 Peptide(PEPTIDE200) C4 5μm 200A 10 and 20mm I.D. s.s. columns 50x10mm 100x10mm 50x10mm 100x10mm HG05100050 15200 HG05100100 15200 HG05100050 15200 HG05100100 15200 50x20mm 100x20mm 50x20mm 100x20mm HG05200050 15200 HG05200100 15200 HG05200050 15200 HG05200100 15200 Peptide(PEPTIDE200) C18 5μm 200A 10 and 20mm I.D. s.s. columns 50x10mm 100x10mm 50x10mm 100x10mm HG05100050 04200 HG05100100 04200 HG05100050 04200 HG05100100 04200 50x20mm 100x20mm 50x20mm 100x20mm HG05200050 04200 HG05200100 04200 HG05200050 04200 HG05200100 04200 PROTEIN300 10mm Semi Prep and 20mm Prep Columns Protein(PROTEIN300) C4 10μm 300A 10 and 20mm I.D. s.s. columns 50x10mm 100x10mm 50x10mm 100x10mm HG10100050 16300 HG10100100 16300 HG10100050 16300 HG10100100 16300 50x20mm 100x20mm 50x20mm 100x20mm HG10200050 16300 HG10200100 16300 HG10200050 16300 HG10200100 16300 Protein(PROTEIN300) C18 10μm 300A 10 and 20mm I.D. s.s. columns 50x10mm 100x10mm 50x10mm 100x10mm HG10100050 05300 HG10100100 05300 HG10100050 05300 HG10100100 05300 50x20mm 100x20mm 50x20mm 100x20mm HG10200050 05300 HG10200100 05300 HG10200050 05300 HG10200100 05300 Protein(PROTEIN300) C4 5μm 300A 10 and 20mm I.D. s.s. columns 50x10mm 100x10mm 50x10mm 100x10mm HG05100050 16300 HG05100100 16300 HG05100050 16300 HG05100100 16300 50x20mm 100x20mm 50x20mm 100x20mm HG05200050 16300 HG05200100 16300 HG05200050 16300 HG05200100 16300 Protein(PROTEIN300) C18 5μm 300A 10 and 20mm I.D. s.s. columns

50x10mm 100x10mm 50x10mm 100x10mm HG05100050 05300 HG05100100 05300 HG05100050 05300 HG05100100 05300 50x20mm 100x20mm 50x20mm 100x20mm HG05200050 05300 HG05200100 05300 HG05200050 05300 HG05200100 05300 Hydrophilic (HYHTA) 10mm Semi Prep and 20mm Prep Columns Hydrophilic(HYHTA) C18 10μm 120A 10 and 20mm I.D. s.s. columns 50x10mm 100x10mm 50x10mm 100x10mm HG10100050 07120 HG10100100 07120 HG10100050 07120 HG10100100 07120 50x20mm 100x20mm 50x20mm 100x20mm HG10200050 07120 HG10200100 07120 HG10200050 07120 HG10200100 07120 Hydrophilic(HYHTA) C18 5μm 120A 10 and 20mm I.D. s.s. columns 50x10mm 100x10mm 50x10mm 100x10mm HG05100050 07120 HG05100100 07120 HG05100050 07120 HG05100100 07120 50x20mm 100x20mm 50x20mm 100x20mm HG05200050 07120 HG05200100 07120 HG05200050 07120 HG05200100 07120

Optimisil Nano and capillary column specifications Material C18 C18 C18 C18 C18 ID ( m)x 1.7μm(120Å) 3μm(120 Å) 3 μm(300 Å) 5 μm(120 Å) 5 μm(300 Å) Length(mm) 25 X 50 CV17N25050 01120 CV03N25050 01120 CV03N25050 01300 CV05N25050 01120 CV05N25050 01300 25 X 100 CV17N25100 01120 CV03N25100 01120 CV03N25100 01300 CV05N25100 01120 CV05N25100 01300 25 X 150 CV17N25150 01120 CV03N25150 01120 CV03N25150 01300 CV05N25150 01120 CV05N25150 01300 25 X 250 CV17N25250 01120 CV03N25250 01120 CV03N25250 01300 CV05N25250 01120 CV05N25250 01300 50 X 50 CV17N50050 01120 CV03N50050 01120 CV03N50050 01300 CV05N50050 01120 CV05N50050 01300 50 X 100 CV17N50100 01120 CV03N50100 01120 CV03N50100 01300 CV05N50100 01120 CV05N50100 01300 50 X 150 CV17N50150 01120 CV03N50150 01120 CV03N50150 01300 CV05N50150 01120 CV05N50150 01300 50 X 250 CV17N50250 01120 CV03N50250 01120 CV03N50250 01300 CV05N50250 01120 CV05N50250 01300 75 X 50 CV17N75050 01120 CV03N75050 01120 CV03N75050 01300 CV05N75050 01120 CV05N75050 01300 75 X 100 CV17N75100 01120 CV03N75100 01120 CV03N75100 01300 CV05N75100 01120 CV05N75100 01300 75 X 150 CV17N75150 01120 CV03N75150 01120 CV03N75150 01300 CV05N75150 01120 CV05N75150 01300 75 X 250 CV17N75250 01120 CV03N75250 01120 CV03N75250 01300 CV05N75250 01120 CV05N75250 01300 100 X 50 CV17M100050 01120 CV03M100050 01120 CV03M100050 01300 CV05M100050 01120 CV05M100050 01300 100 X 100 CV17M100100 01120 CV03M100100 01120 CV03M100100 01300 CV05M100100 01120 CV05M100100 01300 100 X 150 CV17M100150 01120 CV03M100150 01120 CV03M100150 01300 CV05M100150 01120 CV05M100150 01300 100 X 250 CV17M100250 01120 CV03M100250 01120 CV03M100250 01300 CV05M100250 01120 CV05M100250 01300 150 X 50 CV17M150050 01120 CV03M150050 01120 CV03M150050 01300 CV05M150050 01120 CV05M150050 01300 150 X 100 CV17M150100 01120 CV03M150100 01120 CV03M150100 01300 CV05M150100 01120 CV05M150100 01300 150 X 150 CV17M150150 01120 CV03M150150 01120 CV03M150150 01300 CV05M150150 01120 CV05M150150 01300 150 X 250 CV17M150250 01120 CV03M150250 01120 CV03M150250 01300 CV05M150250 01120 CV05M150250 01300 200 X 50 CV17M200050 01120 CV03M200050 01120 CV03M200050 01300 CV05M200050 01120 CV05M200050 01300 200 X 100 CV17M200100 01120 CV03M200100 01120 CV03M200100 01300 CV05M200100 01120 CV05M200100 01300 200 X 150 CV17M200150 01120 CV03M200150 01120 CV03M200150 01300 CV05M200150 01120 CV05M200150 01300 200 X 250 CV17M200250 01120 CV03M200250 01120 CV03M200250 01300 CV05M200250 01120 CV05M200250 01300

Optimisil UHPLC column specifications UHPLC silica packings use high purity (<10 ppm metals), spherical silica with the particle selection of 1.8 and 2.2 μm and the pore size of 120 Å. Their bonded phases include C18, C8, C4, Phenyl, Amino, Cyano, strong cation, and HILIC. Their unique monofunctional bonding chemistry allows high efficiency, high selectivity and high speed separation. All sub 2 micron particles and bonded phases have excellent resistance to high pressure (>10,000 psi). As an example in Figure 1, 2.2 μm MF C18 shows a reduced plate height of 2.22 μm, which is equivalent to plate number of 200,448 per meter. Characteristics Most comprehensive selection of the stationary phases Highly controlled chemistry of monolayer formation and end capping High column to column reproducibility High mechanical stability to resist the pressure as high as up to 10,000 psi High resolution, efficiency and selectivity for separations Suitable for separations of acidic, neutral and basic compounds, peptides, and proteins Specifications Phases Particle size Pore size Surface Carbon ph range (μm) (Å) area loading MF C18 1.8, 2.2 120 300 17.0% 2 8.5 MC C18 1.8, 2.2 120 300 19.0% 1.5 10.5 MF C8 1.8, 2.2 120 300 11.0% 2 8.5 MF C4 1.8, 2.2 120 300 8.0% 2 8.5 MF phenyl 1.8, 2.2 120 300 11.0% 2 8.5 AQ Cyano 1.8, 2.2 120 300 7.0% 2 8.5 AQ SCX 1.8, 2.2 120 300 4.0% 2 8.5 AQ silica 1.8, 2.2 120 300 11.0% 2 8.5 HILIC 1.8, 2.2 120 300 16.0% 2 8.5

Figure 1. Reduced plate height (h) vs. linear flow rate (v). Column: MF C18 (2.2 μm, 4.6x50 mm) Mobile phase: 70% MeOH/30% H2O Temperature: 30 oc Detection: UV 254 nm Test compound: naphthalene Injection: 5 μl

Applications UHPLC series columns can work well with both regular HPLC and UHPLC systems. Figure 2 shows an example of separation of a mixture of test compounds. The flow rate is 0.43 ml/min, generating 3060 psi for a 2.2μm AQ C18 (3.0x150 mm) column. The flow rate was not optimized to reach minimum reduced plate height. However, it showed high separation efficiency and selectivity. Figure 2. Separation of a mixture by regular HPLC system. Column: MF C18 (2.2μm, 3.0x150mm). Mobile phase: 60% Acetonitrile/40% H2O, 0.43 ml/min. Temperature: ambient. Detection: UV 254 nm. Injection: 2 μl.

Figure 3. Separation of a mixture by regular HPLC system. Column: MF C18 (2.2μm, 3.0x150mm). Mobile phase: 60% Acetonitrile/40% H2O, 0.43 ml/min. Temperature: ambient. Detection: UV 254 nm. Injection: 2 μl.

Figure 4. Barbitals separated by a 2.2 μm MF C18 column and a 3 μm commercial C18 column with regular HPLC system. Columns: 4.6x50 mm. Mobile phase: 50% MeOH/50% H2O. Flow rate: 1.0 ml/min. Temperature: 30oC. Detection: UV 214 nm. Injection: 3 μl. Sample: 1. barbital, 2. phenobarbital, 3. aprobarbital, 4. butabarbital, 5. mephobarbital, 6. pentobarbital, and 7. secobarbital Figure 5. Vitamin D2 and D3 separated by a 2.2 μm MF C18 column, a 2.2 μm and a 3 μm C18 columns from other vendors. Columns: 4.6x50 mm. Injection: 3 μl

Optimisil UHPLC column list 1.8μm Reversed and Normal Phases (ID x length mm) Phases 2.1x30 2.1x50 2.1x100 2.1x150 3.0x30 3.0x50 MF-C18 (120 Å) SP-18021030-01120 SP-18021050-01120 SP-18021100-01120 SP-18021150-01120 SP-18030030-01120 SP-18030050-01120 MC-C18 (120 Å) SP-18021030-04120 SP-18021050-04120 SP-18021100-04120 SP-18021150-04120 SP-18030030-04120 SP-18030050-04120 MF-C8 (120 Å) SP-18021030-06120 SP-18021050-06120 SP-18021100-06120 SP-18021150-06120 SP-18030030-06120 SP-18030050-06120 MF-C4 (120 Å) SP-18021030-07120 SP-18021050-07120 SP-18021100-07120 SP-18021150-07120 SP-18030030-07120 SP-18030050-07120 MF-Phenyl (120 Å) SP-18021030-10120 SP-18021050-10120 SP-18021100-10120 SP-18021150-10120 SP-18030030-10120 SP-18030050-10120 AQ-CN (120 Å) SP-18021030-12120 SP-18021050-12120 SP-18021100-12120 SP-18021150-12120 SP-18030030-12120 SP-18030050-12120 AQ-NH2 (120 Å) SP-18021030-11120 SP-18021050-11120 SP-18021100-11120 SP-18021150-11120 SP-18030030-11120 SP-18030050-11120 AQ-SCX (120 Å) SP-18021030-13120 SP-18021050-13120 SP-18021100-13120 SP-18021150-13120 SP-18030030-13120 SP-18030050-13120 AQ-Silica (120 Å) SP-18021030-14120 SP-18021050-14120 SP-18021100-14120 SP-18021150-14120 SP-18030030-14120 SP-18030050-14120 HILIC Polar SP-18021030-27120 SP-18021050-27120 SP-18021100-27120 SP-18021150-27120 SP-18030030-27120 SP-18030050-27120

Optimisil 1.8μm Reversed and Normal Phases (ID x length mm Phases 3.0x100 3.0x150 4.6x30 4.6x50 4.6x100 4.6x150 MF-C18 (120 Å) SP-18030100-01120 SP-18030150-01120 SP-18046030-01120 SP-18046050-01120 SP-18046100-01120 SP-18046150-01120 MC-C18 (120 Å) SP-18030100-04120 SP-18030150-04120 SP-18046030-04120 SP-18046050-04120 SP-18046100-04120 SP-18046150-04120 MF-C8 (120 Å) SP-18030100-06120 SP-18030150-06120 SP-18046030-06120 SP-18046050-06120 SP-18046100-06120 SP-18046150-06120 MF-C4 (120 Å) SP-18030100-07120 SP-18030150-07120 SP-18046030-07120 SP-18046050-07120 SP-18046100-07120 SP-18046150-07120 MF-Phenyl (120 Å) SP-18030100-10120 SP-18030150-10120 SP-18046030-10120 SP-18046050-10120 SP-18046100-10120 SP-18046150-10120 AQ-CN (120 Å) SP-18030100-12120 SP-18030150-12120 SP-18046030-12120 SP-18046050-12120 SP-18046100-12120 SP-18046150-12120 AQ-NH2 (120 Å) SP-18030100-11120 SP-18030150-11120 SP-18046030-11120 SP-18046050-11120 SP-18046100-11120 SP-18046150-11120 AQ-SCX (120 Å) SP-18030100-13120 SP-18030150-13120 SP-18046030-13120 SP-18046050-13120 SP-18046100-13120 SP-18046150-13120 AQ-Silica (120 Å) SP-18030100-14120 SP-18030150-14120 SP-18046030-14120 SP-18046050-14120 SP-18046100-14120 SP-18046150-14120 HILIC Polar SP-18030100-27120 SP-18030150-27120 SP-18046030-27120 SP-18046050-27120 SP-18046100-27120 SP-18046150-27120

Optimisil 2.2μm Reversed and Normal Phases (ID x length mm) Phases 2.1x30 2.1x50 2.1x100 2.1x150 3.0x30 3.0x50 MF-C18 (120 Å) SP-22021030-01120 SP-22021050-01120 SP-22021100-01120 SP-22021150-01120 SP-22030030-01120 SP-22030050-01120 MC-C18 (120 Å) SP-22021030-04120 SP-22021050-04120 SP-22021100-04120 SP-22021150-04120 SP-22030030-04120 SP-22030050-04120 MF-C8 (120 Å) SP-22021030-06120 SP-22021050-06120 SP-22021100-06120 SP-22021150-06120 SP-22030030-06120 SP-22030050-06120 MF-C4 (120 Å) SP-22021030-07120 SP-22021050-07120 SP-22021100-07120 SP-22021150-07120 SP-22030030-07120 SP-22030050-07120 MF-Phenyl (120 Å) SP-22021030-10120 SP-22021050-10120 SP-22021100-10120 SP-22021150-10120 SP-22030030-10120 SP-22030050-10120 AQ-CN (120 Å) SP-22021030-12120 SP-22021050-12120 SP-22021100-12120 SP-22021150-12120 SP-22030030-12120 SP-22030050-12120 AQ-NH2 (120 Å) SP-22021030-11120 SP-22021050-11120 SP-22021100-11120 SP-22021150-11120 SP-22030030-11120 SP-22030050-11120 AQ-SCX (120 Å) SP-22021030-13120 SP-22021050-13120 SP-22021100-13120 SP-22021150-13120 SP-22030030-13120 SP-22030050-13120 AQ-Silica (120 Å) SP-22021030-14120 SP-22021050-14120 SP-22021100-14120 SP-22021150-14120 SP-22030030-14120 SP-22030050-14120 HILIC Polar SP-22021030-27120 SP-22021050-27120 SP-22021100-27120 SP-22021150-27120 SP-22030030-27120 SP-22030050-27120

Optimisil 2.2μm Reversed and Normal Phases (ID x length mm) Phases 3.0x100 3.0x150 4.6x30 4.6x50 4.6x100 4.6x150 MF-C18 (120 Å) SP-22030100-01120 SP-22030150-01120 SP-22046030-01120 SP-22046050-01120 SP-22046100-01120 SP-22046150-01120 MC-C18 (120 Å) SP-22030100-04120 SP-22030150-04120 SP-22046030-04120 SP-22046050-04120 SP-22046100-04120 SP-22046150-04120 MF-C8 (120 Å) SP-22030100-06120 SP-22030150-06120 SP-22046030-06120 SP-22046050-06120 SP-22046100-06120 SP-22046150-06120 MF-C4 (120 Å) SP-22030100-07120 SP-22030150-07120 SP-22046030-07120 SP-22046050-07120 SP-22046100-07120 SP-22046150-07120 MF-Phenyl (120 Å) SP-22030100-10120 SP-22030150-10120 SP-22046030-10120 SP-22046050-10120 SP-22046100-10120 SP-22046150-10120 AQ-CN (120 Å) SP-22030100-12120 SP-22030150-12120 SP-22046030-12120 SP-22046050-12120 SP-22046100-12120 SP-22046150-12120 AQ-NH2 (120 Å) SP-22030100-11120 SP-22030150-11120 SP-22046030-11120 SP-22046050-11120 SP-22046100-11120 SP-22046150-11120 AQ-SCX (120 Å) SP-22030100-13120 SP-22030150-13120 SP-22046030-13120 SP-22046050-13120 SP-22046100-13120 SP-22046150-13120 AQ-Silica (120 Å) SP-22030100-14120 SP-22030150-14120 SP-22046030-14120 SP-22046050-14120 SP-22046100-14120 SP-22046150-14120 HILIC Polar SP-22030100-27120 SP-22030150-27120 SP-22046030-27120 SP-22046050-27120 SP-22046100-27120 SP-22046150-27120