Annexure V PROTOCOL FOR LST
Numer of lotions for LST. Cotton hyrids Zones* Souther n Centrl Norther n H X B 0 0 - X 0 0 5 H X H 0 0 0 H X 0 0 - H hirsutum; B rdne; h hereum; rorium *To e optimlly divided etween irrigted, rin-fed nd suppressive soils (the tril my lso ommodte oth norml sown nd lte sown onditions)
Field note Book* rop yer Dte of sowing Dte of hrvest Below 0.5h trils LST size Su-plot size 5x5m three or more Plot size (5x5m)* or n, n = numer of ndidtes + ommeril Bt hyrid + ommeril hyrid if ny or nother relesed Bt. otton 60-90 m (depending on the genotype) Spe etween plnt to plnt Spe etween row to row 90-0 m ( ) Spe etween replition m Qudrnt omprises of four plnts (two eh of djent rows) Row length (m) ( 5 m ) otton plnt line Row width (m) ( 5 m ) Spe etween experimentl re nd refuge/order m - row - Dt to e monitored on three fixed qudrnts in eh su-plot. All oservtion relted to pest predtor noted on this. Eh qudrnt should e tlest meters prt from one nother nd from the order Speifitions on entries Bt otton tests hyrids Anlogues non-bt otton hyrids (wherever ville) Speifitions on heks Reently relesed Bt otton s hek (zone wise) Regionl ( non Bt otton hyrid of the zone)
Yield Dt Book Prt A. Pest-predtor dynmis
Tle A- : Summry of Lrvl Counts of Bollworm omplex inluding Spodopter in LST Lrvl ount ( lrve / plt - men 50 plts / entry) t 60,75, 90.05,0 & 5 DAS Sl. Hyrid Spotted Bollworm Amerin Bollworm Pink Bollworm Spodopter No. 60 90 0 5 60 90 0 5 60 90 0 5 60 90 0 5 ` Qudrt (Lotions ) Qudrt (Lotions ) Qudrt (Lotions ) Men : (Lotions )
Tle A-: Summry of dmge in fruiting odies, Open oll & Loule over lotions Zone Dmge (%) per qudrt Sl. Hyrid Fruiting odies Open oll Loule No. 60 90 0 5 ` Qudrt : (Lotions ) Qudrt : (Lotions ) Qudrt : (Lotions ) Men (Lotions )
Tle A-: Summry of Suking Pest Popultion of G.M. otton hyrids & heks over lotions Zone Suking Pests/per qudrt Sl. Hyrid Aphid Jssid Thrip Whitefly No. 60 90 0 5 60 90 0 5 60 90 0 5 60 90 0 5 ` Qudrt (Lotions ) Qudrt (Lotions ) Qudrt (Lotions ) Zone : (Lotions ) Men
Tle A-: Summry of Benefiil Inset Popultion of G.M. otton hyrids & heks Benefiil Insets Sl. Hyrid Cndidte Bt Seond Bt Bt. Chek Hyrid No. 60 90 0 5 60 90 0 5 60 90 0 5 60 90 0 5 ` (Lotions ) Qudrt Coinellids Chrysop Syrphids Spider (Lotions ) Qudrt (Lotions ) Qudrt
Tle A-5: Summry of Virl, teril & fungl diseses inidene of G.M. otton hyrids & heks over Zone Inidene (%) (DAS) Sl. Hyrid Cotton Lef Curl Virus Bteril Blight Wilt Any other (Speify) No. 60 90 0 5 60 90 0 5 60 90 0 5 60 90 0 5 ` Qudrt (Lotions ) Bt Cotton A Bt Cotton B Non Bt Chek of A Non Bt Chek of B Hyrid Qudrt (Lotions ) Qudrt (Lotions )
Yield Dt Book Prt B. Yield dt
Field note Book - B Tle B-: LST gronomi trits of G.M. otton hyrids & heks Lotion Code - SZ/LST/8-005k Sl. Hyrid Germi- Plnts/ Bolls/ Boll Sym- Mono- Plnt Dys Brt- Seed Cotton Yield Lint No. test mteril ntion plot plnt Weight podi/ podi/ Height to 50% lett's % over Yield (%) (g) plnt plnt (m) flowering Index (Qtl/h) est hek (Qtl/h) ` (Lotions ) ` (Lotions ) ` (Lotions ) ` (Lotions )
Field note Book - B Tle B-: Summry of Brtlett's Index & Piking wise Yield of G. M. Cotton Hyrid nd hek. Sl. Hyrid Dte Seed Cotton Yield - Kg / piking (P) (DAS) Totl Brt- No. of I II III IV V VI (finl) Totl Yield lett's sow ing 0 5 50 65 80 >80 (Kg) (Qtl/h) Index ` Entry : (Lotions ) ` Entry : (Lotions ) ` Entry : (Lotions ) ` Entry : (Lotions ) Men Brtlett's Index ( B.I. ) = ( 6 x I P + 5 x II P + x III P + x IV P + x V P + x VI P )
Yield Dt Book Prt-C. Fier nd oil detils (see text for smple size nd uthenti lortory for testing on pyment sis)
Field note Book - C Tle C- : *Summry of fire properties & oil (%) of G.M. otton hyrids & heks from three LST (eh is men of 5 oservtions). Zone :.5% Unifor- Fineness Strength Spinn- Count Sl. Lotion / Ginning Seed Lint Spn mity. mm le Strength Oil No. Entry out-turn Index Index length rtio (Miro- guge ounts Produt (%) (g) (g) (mm) (%) nire) (g/tex) (CSP) (%) ` (Lotions ) LST- Entry A Entry B Entry C hek ` (Lotions ) LST- Entry A Entry B Entry C hek ` (Lotions ) LST- Entry A Entry B Entry C hek ` LST- LST-5 *To e done y CCTI, Mthung, Mumi (Fee to e deided y the Institute