HICHROM Chromatography Columns and Supplies LC COLUMNS Inertsil Catalogue 9 The Markham Centre, Station Road Theale, Reading, Berks, RG7 PE, UK Tel: + (0)8 90 660 Fax: + (0)8 9 8 Email: sales@hichrom.co.uk www.hichrom.co.uk
Please contact Hichrom for current prices. Tel: + (0)8 90 660 Email: sales@hichrom.co.uk GL Sciences Inertsil Series Enhanced sensitivity High resolution Excellent for acidic, basic and chelating compounds Compatible with up to 00% aqueous eluents Inertsil Series Phases Phase Endcapped Particle Size (μm) Pore Size (Å) Surface Area (m /g) Carbon Load (%) ODS- and ODS-V Yes,, 00 0 C8- Yes,, 00 0 The Inertsil series is based on enhanced deactivation techniques which produce highly inert silica, enabling successful analysis of trace levels of the most demanding basic and acidic compounds. Strictly classified particle size, with narrow distribution width and an ideal carbon loading, enables high efficiency columns to be guaranteed. Figure illustrates the high sensitivity LC-MS/MS separation of haloacetic acids, harmful substances generated when tap water is chlorinated. Inertsil ODS- and C8- columns also show excellent stability during the analysis of strong metal chelating compounds. Figure shows the separation of tetracyclines, veterinary antibiotics with very strong chelating characteristics. The μm phases are also available in High Pressure (HP) format. Please enquire for details. Column: Inertsil ODS- (μm, 0 x.mm) Eluent: A: CH OH B: 0.% HCOOH Gradient: A/B = /9.min /9.min 9/ min 9/ Flow rate: 0.ml/min Temperature: 0 C 6 Detection: LC-MS/MS (ESI, negative, MRM). Bromoacetic acid. Dibromoacetic acid. Tribromoacetic acid. Chloroacetic acid. Dichloroacetic acid 6. Trichloroacetic acid 7. Bromochloroacetic acid Column: Inertsil ODS- (μm, 7 x.mm) Eluent: CH OH B: 0.% HCOOH Gradient: A/B = 0/80.min 0/80 min 9/.min 9/ Flow rate: 0.ml/min Temperature: 0 C Detection: LC-MS/MS (ESI, positive, MRM). Tetracycline (TC). Oxytetracycline (OTC). Chlortetracycline (CTC). Doxycycline (DC) 7 0 6 7 8 9 0 Mins 0 0..0..0..0..0..0. 6.0 6. 7.0 7. 8.0 8. 9.0 9. Mins Figure. Analysis of haloacetic acids by LC-MS/MS Figure. Analysis of tetracyclines by LC-MS/MS Ordering Information Inertsil Series Column Dimensions (mm) Guard Cartridges (/pk) 0 x. 0 x. 7 x. 00 x. 0 x. 0 x. (0 x.mm) μm 68 8 - - ODS- 00-800 00-80 00-80 00-80 00-80 - - C8-00-880 00-88 00-88 00-88 00-88 - - μm 7 79 8 ODS- 00-00 00-00 00-00 00-00 00-00 00-006 00-90 C8-00-097 00-097 00-097 00-097 00-097 00-0976 00-97 - 79 9 96 7 - ODS-V - 00-0 00-0 00-0 00-0 00-06 - μm 79 79 9 9 7 8 ODS- 00-09 00-09 00-09 00-09 00-09 00-096 00-90 C8-00-00 00-00 00-00 00-00 00-00 00-006 00-96 Column Dimensions (mm) Guard Cartridges (/pk) 0 x.6 0 x.6 7 x.6 00 x.6 0 x.6 0 x.6 (0 x.0mm) μm 7 79 6 ODS- 00-00 00-00 00-00 00-00 00-00 00-006 00-900 C8-00-099 00-099 00-099 00-099 00-0996 00-0997 00-907 - 79 9 96 7 - ODS-V - 00-0 00-0 00-0 00-0 00-06 - μm 79 79 9 9 7 6 ODS- 00-09 00-09 00-09 00-09 00-09 00-096 00-900 C8-00-008 00-008 00-008 00-008 00-008 00-0086 00-906 - - - - 79 8 - ODS-V - - - - 00-090 00-090 - Other dimensions available Use with guard cartridge holder 00-0800 ( 97) www.hichrom.co.uk sales@hichrom.co.uk Tel: 08 90 660 07
GL Sciences Inertsil Series High purity base silica (99.999%) Large surface area Exceptional stability and column lifetime Validated ODS- column Compared with Inertsil Series, Inertsil Series phases are based on a purer, higher surface area silica which is specially manufactured to provide maximum phase coverage. The result is a series of columns which provide excellent peak shapes using simple eluents while operating at low pressure. Inertsil Series Phases Phase Endcapped Particle Size (μm) Pore Size (Å) Surface Area (m /g) Carbon Load (%) Recommended ph Range* SIL -, 00 0 - - 7. ODS- Yes,,,, 0 00 0-7. ODS-V Yes, 00 0-7. ODS-P No, 00 0 9-7. ODS-EP No 00 0 9-7. Peptide C8 Yes 00 0-7. ODS-Sprint Yes, 00 0 8. - 7. Sulfa C8 Yes, 00 0-7. C8- Yes,, 00 0 9-7. Ph- No,, 00 0 9. - 7. CN- No, 00 0-7. NH No, 00 0 8-7. Diol No, 00 0-7. HILIC No, 00 0 0-7. Amide No, 00 0 8-7. AX No 00 0 7-7. CX No 00 0-7. *Inertsil phases are known to provide excellent results at ph levels of 9 0. However, optimum column life may be achieved at a ph between and 7.. Inertsil ODS- Peak tailing of basic components in reversed-phase is frequently the result of residual silanol groups. GL Sciences developed a proprietary bonding endcapping technology to eliminate these silanols. To ensure maximum inertness, each batch of Inertsil ODS- must pass a QC test using 9 Si NMR. After ODS bonding, the magnitude of the isolated silanols is greatly decreased. After endcapping, the isolated silanols have been completely eliminated, thereby assuring excellent inertness and optimum performance of ODS- (see Figure ). Isolated silanol Si-OH Silica-gel structure Si-(O-Si) ODS group C 8 H 7 -Si-O- Remaining silanol Endcapped groups (CH ) -Si-O- Geminal silanol Si-(OH) For free technical advice and support email technical@hichrom.co.uk Bare silica gel support After ODS bonding After ODS bonding and endcapping Figure. Measurement of residual silanol groups by 9 Si NMR Inertsil ODS-V a validated Inertsil column Inertsil ODS-V is a highly efficient, validated HPLC column, developed to provide optimum separation for a wide range of acidic and basic compounds. Each lot of packing undergoes a series of critical QC analytical tests, consistent with the demands of GLP/GMP compliance, to confirm: ) the absence of surface silanols ) minimal metallic residues ) inertness to basic compounds ) reproducible separations. Norephedrine. Nortriptyline. Toluene. Imipramine. Amitriptyline Column dimensions: 0 x.6mm Flow rate: ml/min. Eluent: CH OH - mm KH PO, ph 6 (60:0) Temperature: C Figure 6. Separation of antidepressant drugs on Inertsil ODS-V 0 6 8 0 6 8 Mins 08
GL Sciences Inertsil Series (continued) Inertsil ODS-P Polymerically bonded C8 Improved separation of planar compounds Useful for carotenoid analysis Separation of the 6 EPA PAHs Inertsil ODS-P is a polymerically bonded phase which is densely bonded with octadecylsilane groups. It shows unique separation capabilities and offers an alternative selectivity to Inertsil ODS-. Inertsil ODS-P is particularly suited to the separation of polycyclic aromatic hydrocarbons and shows additional retentivity for planar compounds. Figure 7 shows the separation of terphenyl isomers on three types of ODS phases. On the polymeric Inertsil ODS-P phase, the planar p-terphenyl is retained longer than the non-planar o- and m- isomers. Figure 8 shows the separation of components from toad venom on the ODS-P phase. o-terphenyl (non-planar) m-terphenyl (non-planar) p-terphenyl (planar) m- m- m- Column dimensions: 0 x.6mm Eluent: CH CN - H O (8:) Flow rate: ml/min Temperature: 0 C Detection: UV, nm o- p- o- p- o- p- 0 6 8 0 0 6 8 0 0 6 8 Mins Monomeric (Inertsil ODS-) Intermediate (Inertsil ODS-) Polymeric (Inertsil ODS-P) Figure 7. Separation of terphenyl isomers Column: Inertsil ODS-P (0 x.6mm) Eluent: CH CN - 0.% H PO (:) Flow rate: ml/min Temperature: 0 C. Bufalin. Resibufogenin. Cinobufagin. Indomethacin 0 0 0 0 Mins Figure 8. Separation of toad venom Inertsil ODS-EP C8 with polar embedded group Compatible with 00% aqueous eluents Alternative selectivity to C8 Ideal for LC-MS Inertsil ODS-EP Uracil Column dimensions: 0 x.6mm Eluent: CH CN - H O (70:0) Flow rate: ml/min Column temperature: 0 C Figure 9. Comparison of Inertsil ODS-EP and C8 columns* *The comparative separations presented here may not be representative for all applications. Inertsil ODS-EP contains a polar functional group embedded between the silica surface and the C8 group. This creates a stable phase, which is resistant to phase collapse (or pore dewetting) in 00% aqueous eluents. It provides highly reproducible separations for acids and bases in organic eluents as well as acidified eluents typically used in LC-MS. Figure 9 shows a comparison of the selectivity of Inertsil ODS-EP with that of a C8 phase. Ethylbenzene Naphthalene Propylbenzene Butylbenzene Anthracene Uracil Naphthalene Ethylbenzene Propylbenzene Alkyl-C8 Anthracene Butylbenzene www.hichrom.co.uk sales@hichrom.co.uk Tel: 08 90 660 09
GL Sciences Inertsil Series (continued) Inertsil ODS-Sprint Balanced retention of polar and non-polar compounds Compatible with 00% aqueous eluents Low back pressure Inertsil ODS-Sprint is super base deactivated and optimally bonded to retain polar compounds without excessive retention of non-polar compounds. This phase shows excellent peak shapes for a wide range of polar analytes, including both basic and acidic compounds. In Figure 0 the hydrophobic compounds are eluted in approximately two-thirds the time of Inertsil ODS-, whilst maintaining retention of polar compounds. Inertsil ODS-Sprint operates at low back pressures, even at high flow rates and shows excellent robustness and reproducibility. Column: Inertsil ODS-Sprint and Inertsil ODS- Column dimensions: 0 x.6mm Eluent: CH OH - H O (80:0) Flow rate: ml/min Temperature: 0 C Inertsil ODS-Sprint 6 7. Uracil. Caffeine. Phenol. n-butylbenzene. o-terphenyl 6. n-amylbenzene 7. Triphenylene Column: Inertsil Peptide C8 (00 x.mm) Eluent: A: H O - CH CN (9:) + 0.0% TFA B: CH CN - H O (80:0) + 0.0% TFA Gradient: - 0% B in 0 mins Flow rate: 0.ml/min Sample: 60pmol cytochrome C tryptic digest 7 Inertsil ODS- 6 0 0 0 0 Mins 0 0 0 0 Mins Figure 0. Polar and non-polar mixture Figure. Analysis of cytochrome C tryptic digest on Inertsil Peptide C8 Inertsil Peptide C8 The Inertsil Peptide C8 column is specifically suited to the analysis of peptide digests. Figure shows the application of this column to the analysis of cytochrome C tryptic digest. Inertsil Sulfa C8 Inertsil Sulfa C8 ( and μm) is designed for the analysis of sulpha drugs. In addition, it is effective in the screening of synthetic antibacterial agents and residues in products of animal origin, as directed by food safety investigative guidelines. Each batch of Inertsil Sulfa C8 is tested for the effective separation of sulpha drugs, as shown in Figure. For free technical advice and support email technical@hichrom.co.uk Figure. Analysis of sulfa reagents 6 7 8 9 0 0 0 0 0 Mins 0 Column: Inertsil Sulfa C8 (µm, 0 x.6mm) Eluent: A: CH CN CH CO H - (777.66:., w/w) B: CH CO H H O (.:997, w/w) 00% B to 67% B in mins then to 0% B in mins Flow rate: ml/min Temperature: 0 C Sample: ppm each. Sulfanilamide. Sulfacetamide. Sulfadiazine. Sulfathiazole. Sulfapyridine 6. Sulfameradine 7. Sulfacymidine 8. Sulfamethoxypyridazine 9. Sulfamonomethoxine 0. Sulfachlorpyridazine. Sulfamethoxazole. Sulfabenzamide. Sulfadimethoxine. Sulfaquinoxaline. Sulfanitran 0
GL Sciences Inertsil Series (continued) Inertsil HILIC Unique selectivity for polar compounds High sensitivity LC-MS analyses Low back pressure Applications for amino acids, water-soluble vitamins and drug metabolites Inertsil HILIC is a propyl alcohol bonded silica phase, suitable for the analysis of polar compounds in the hydrophilic interaction (HILIC) mode. It has also shown promise in SFC applications. Unlike traditional reversed-phase materials, HILIC columns retain highly polar compounds with only small amounts of water in the eluent. The high organic solvent composition of the eluent will lead to high sensitivity LC-MS analyses, due to a decrease in ion suppression. It also results in low back pressure and the ability to run at high flow rates. Figure shows the separation of a mixture of oligosaccharides on Inertsil HILIC. Inertsil HILIC has also been used in the development of a fast and sensitive LC-MS/MS method for the analysis of melamine and cyanuric acid contaminants in pet food samples (see Figure ). Column: Inertsil HILIC (µm, 0 x.6mm) Eluent: CH CN - H O (6:) Flow rate: ml/min Temperature: 0 C. Maltose. Maltotriose. Maltotetraose. Maltopentaose. Maltohexaose 6. Maltoheptaose Column: Inertsil HILIC (0 x.0mm) Eluent: A: 0mM ammonium acetate in CH CN B: 0mM ammonium acetate in H O Gradient: T(mins) %A %B 0 97.0 0 80. 97 Flow rate: 0.ml/min Temperature: 0 C Detection: MS/MS (ESI) 6 Cyanuric Acid (Negative ESI) Melamine (Positive ESI) 0 6 8 Mins 0 0.0..0..0. Mins Figure. Analysis of oligosaccharides on Inertsil HILIC Figure. Analysis of melamine and cyanuric acid on Inertsil HILIC Inertsil Amide Excellent for polar compounds in HILIC mode Ideal for LC-MS/MS Long column lifetime Inertsil Amide is a carbamoyl bonded silica phase designed for the separation of highly polar compounds by HILIC. Inertsil Amide columns offer significantly increased retentivity and a unique selectivity in separating compounds that were unresolvable on other columns. Column: Inertsil Diol (0 x.6mm) Eluent: CH CN - H O (80:0) Flow rate: ml/min Column temperature: 0 C Inertsil Diol Unique selectivity in both reversed-phase and normal-phase Applications for amino acids, water-soluble vitamins and sugars 6. Phenylalanine. Methionine. Valine. Dopa. Alanine 6. Serine 7. Citrulline 7 The Inertsil Diol phase is a dihydroxypropyl bonded silica which can be used in both reversed-phase and normal-phase modes. Figure shows the analysis of free amino acids on an Inertsil Diol column. Figure. Analysis of free amino acids www.hichrom.co.uk sales@hichrom.co.uk Tel: 08 90 660
Please contact Hichrom for current prices. Tel: + (0)8 90 660 Email: sales@hichrom.co.uk GL Sciences Inertsil Series (continued) Inertsil Ion-Exchange Phases Silica based ion-exchange columns High reproducibility High efficiency Exceptional stability and long column lifetime Inertsil AX and CX are silica based anion- and cation-exchange phases respectively, based on high purity silica. They show good reproducibility, exceptional stability and long column lifetime. Figure 6 shows the separation of nucleotides on Inertsil AX and Figure 7 shows the analysis of nucleic acid bases by cation-exchange chromatography on Inertsil CX. Column: Inertsil AX (0 x.6mm) Eluent: 0.0M KH PO (ph.0 with H PO ) Flow rate: ml/min Temperature: 0 C N(CH CH ) C 6 H SO - H + AX bonded phase structure CX bonded phase structure Column: Inertsil CX (0 x.6mm) Eluent: 0.M ammonium formate, ph.0 Flow rate: ml/min Temperature: 0 C. CMP. AMP. UMP. GMP. XMP. Uracil. Thymine. Guanine. Cytosine. Adenine 0 0 0 0 Mins 0 0 0 Mins 0 Figure 6. Analysis of nucleotides on Inertsil AX Figure 7. Analysis of nucleic acid bases on Inertsil CX Ordering Information Inertsil Series Inertsil ODS- (Hichrom Manufactured Columns) Column Dimensions (mm) 00 x.6 0 x.6 0 x.6 ODS- μm INODS--00D 07 INODS--0D 9 INODS--0D 6 ODS- μm INODS-00D INODS-0D 7 INODS-0D 07 Please contact Hichrom for availability of other Series phases and alternative dimension columns. Microbore (.mm i.d.) Columns (GL Sciences Manufactured) Column Dimensions (mm) 0 x. 0 x. 7 x. 00 x. 0 x. μm 9 09 97 70 C8-00-890 00-89 00-89 00-89 00-89 ODS- 00-860 00-86 00-86 00-86 00-86 Ph- 00-80 00-8 00-8 00-8 00-8 For free technical advice and support email technical@hichrom.co.uk Column Dimensions (mm) Guard Cartridges (/pk) 0 x. 00 x. 0 x. 0 x. (For.mm i.d. Columns) μm 8 07 9 6 8 C8-00-08 00-08 00-08 00-086 00-9 ODS- 00-0 00-0 00-0 00-06 00-90 ODS-P 00-066 00-066 00-066 00-0666 00-909 ODS-Sprint 00-08 00-08 00-08 00-086 00-907 Ph- 00-00 00-00 00-00 00-006 00-97 CN- 00-06 00-06 00-06 00-066 00-99 NH 00-06 00-06 00-06 00-066 00-9 Diol 00-0 00-0 00-0 00-06 00-9 SIL 00-0 00-0 00-0 00-06 00-97 HILIC 00-0776 00-0776 00-0776 00-07766 00-9 Amide 00-0786 00-0786 00-0786 00-07866 00-060 Other column dimensions available Use with free standing holder 00-0800 ( 97)
Please contact Hichrom for current prices. Tel: + (0)8 90 660 Email: sales@hichrom.co.uk GL Sciences Ordering Information Inertsil Series (continued) Microbore (.mm i.d.) Columns (GL Sciences Manufactured) (continued) Column Dimensions (mm) 0 x. 00 x. 0 x. 0 x. Guard Cartridges (/pk) (For.mm i.d. Columns) μm 9 7 8 8 ODS-V 00-0 00-0 00-0 00-06 00-90 6 8 09 8 Sulfa C8 00-070 00-070 00-070 00-0706 00-9 μm 7 79 0 8 ODS- 00-06 00-06 00-06 00-066 00-90 μm 0 7 07 8 C8-00-09 00-09 00-09 00-096 00-9 ODS- 00-0 00-0 00-07 00-07 00-90 ODS-P 00-07 00-07 00-07 00-076 00-908 ODS-EP 00-06 00-06 00-06 00-066 00-90 ODS-Sprint 00-07 00-07 00-07 00-076 00-906 Ph- 00-0 00-0 00-0 00-06 00-96 CN- 00-0 00-0 00-0 00-06 00-98 NH 00-0 00-0 00-0 00-06 00-90 Diol 00-06 00-06 00-06 00-066 00-9 SIL 00-0 00-0 00-0 00-06 00-96 HILIC 00-0770 00-0770 00-0770 00-07706 00-9 Amide 00-0780 00-0780 00-0780 00-07806 00-09 78 07 9 6 8 Sulfa C8 00-07 00-07 00-07 00-076 00-9 8 9 98 8 AX 00-07 00-07 00-07 00-076 00-9 0 8 0 0 8 CX 00-07 00-07 00-07 00-076 00-9 78 7 80 8 Peptide C8 00-080 00-080 00-080 00-0806 00-9 Medium bore (.0mm i.d.) Columns (GL Sciences Manufactured) Column Dimensions (mm) 0 x.0 0 x.0 7 x.0 00 x.0 0 x.0 μm 9 09 97 70 C8-00-89 00-896 00-897 00-898 00-899 ODS- 00-8660 00-866 00-866 00-866 00-866 Ph- 00-8 00-86 00-87 00-88 00-89 Column Dimensions (mm) Guard Cartridges (/pk) 0 x.0 00 x.0 0 x.0 0 x.0 (For.0mm i.d. Columns) μm 8 07 9 6 6 C8-00-08 00-08 00-08 00-086 00-9 ODS- 00-0 00-0 00-0 00-06 00-90 ODS-P 00-067 00-067 00-067 00-0676 00-909 ODS-Sprint 00-08 00-08 00-08 00-086 00-907 Ph- 00-00 00-00 00-00 00-006 00-97 CN- 00-07 00-07 00-07 00-076 00-99 NH 00-07 00-07 00-07 00-076 00-9 Diol 00-0 00-0 00-0 00-06 00-9 SIL 00-0 00-0 00-0 00-06 00-97 HILIC 00-0777 00-0777 00-0777 00-07776 00-9 Amide 00-0787 00-0787 00-0787 00-07876 00-0 9 7 8 6 ODS-V 00-0 00-0 00-0 00-06 00-90 6 8 09 6 Sulfa C8 00-07 00-07 00-07 00-076 00-9 μm 7 79 0 6 ODS- 00-06 00-06 00-06 00-066 00-90 Other column dimensions available Use with free standing holder 00-0800 ( 97) www.hichrom.co.uk sales@hichrom.co.uk Tel: 08 90 660
Please contact Hichrom for current prices. Tel: + (0)8 90 660 Email: sales@hichrom.co.uk GL Sciences For free technical advice and support email technical@hichrom.co.uk Ordering Information Inertsil Series (continued) Medium bore (.0mm i.d.) Columns (GL Sciences Manufactured) (continued) Column Dimensions (mm) Guard Cartridges (/pk) 0 x.0 00 x.0 0 x.0 0 x.0 (For.0mm i.d. Columns) μm 0 7 07 6 C8-00-09 00-09 00-09 00-096 00-9 ODS- 00-0 00-0 00-07 00-07 00-90 ODS-P 00-07 00-07 00-07 00-076 00-908 ODS-EP 00-06 00-06 00-06 00-066 00-90 ODS-Sprint 00-07 00-07 00-07 00-076 00-906 Ph- 00-0 00-0 00-0 00-06 00-96 CN- 00-0 00-0 00-0 00-06 00-98 NH 00-0 00-0 00-0 00-06 00-90 Diol 00-06 00-06 00-06 00-066 00-9 SIL 00-0 00-0 00-0 00-06 00-96 HILIC 00-077 00-077 00-077 00-0776 00-9 Amide 00-078 00-078 00-078 00-0786 00-0 - - 9 68 6 ODS-V - - 00-08 00-08 00-90 78 07 9 6 6 Sulfa C8 00-07 00-07 00-07 00-076 00-9 8 9 98 6 AX 00-07 00-07 00-07 00-076 00-9 0 8 0 0 6 CX 00-07 00-07 00-07 00-076 00-9 78 7 80 6 Peptide C8 00-080 00-080 00-080 00-0806 00-9 Analytical (.6mm i.d.) Columns (GL Sciences Manufactured) Column Dimensions (mm) Guard Cartridges (/pk) 0 x.6 00 x.6 0 x.6 0 x.6 (For.6mm i.d. Columns) μm 8 07 9 6 6 C8-00-08 00-08 00-09 00-09 00-90 ODS- 00-077 00-077 00-077 00-077 00-900 ODS-P 00-069 00-069 00-069 00-0696 00-9009 ODS-Sprint 00-08 00-08 00-08 00-086 00-9007 Ph- 00-00 00-00 00-09 00-09 00-907 CN- 00-09 00-09 00-09 00-096 00-909 NH 00-09 00-09 00-09 00-096 00-90 Diol 00-0 00-0 00-0 00-06 00-90 SIL 00-0 00-0 00-070 00-070 00-907 HILIC 00-0779 00-0779 00-0779 00-07796 00-90 Amide 00-0789 00-0789 00-0789 00-07896 00-08 9 7 8 6 ODS-V 00-0 00-0 00-0 00-06 00-900 Sulfa C8 00-07 ( ) 00-07 ( 6) 00-07 ( 8) 00-076 ( 09) 00-90 μm 7 79 0 6 ODS- 00-06 00-06 00-06 00-066 00-900 μm 0 7 07 6 C8-00-09 00-09 00-0900 00-090 00-90 ODS- 00-076 00-076 00-07 00-07 00-900 ODS-P 00-07 00-07 00-000 00-000 00-9008 ODS-EP 00-06 00-06 00-06 00-066 00-900 ODS-Sprint 00-07 00-07 00-07 00-076 00-9006 Ph- 00-0 00-0 00-090 00-09 00-906 CN- 00-0 00-0 00-090 00-09 00-908 NH 00-0 00-0 00-0 00-06 00-900 Diol 00-06 00-06 00-06 00-066 00-90 SIL 00-0 00-0 00-07 00-07 00-906 HILIC 00-077 00-077 00-077 00-0776 00-90 Amide 00-078 00-078 00-078 00-0786 00-07 78 07 9 68 6 ODS-V - - 00-080 00-080 00-900 Sulfa C8 00-077 00-077 00-077 00-0776 ( 6) 00-90 8 9 98 6 AX 00-07 00-07 00-07 00-076 00-90 0 8 0 0 6 CX 00-07 00-07 00-07 00-076 00-90 78 7 80 6 Peptide C8 00-080 00-080 00-080 00-0806 00-90 Other column dimensions available Use with free standing holder 00-0800 ( 97)
GL Sciences Inertsil Wide Pore Phases High purity 00Å silica phases Excellent reproducibility Good physical and chemical stability Inertsil Wide Pore Phases Inertsil WP00 Particle Size Surface Area Carbon Load Bonding Endcapped Pore Size (Å) Phase (μm) (m /g) (%) ph Range SIL - - 00 0 - - 7. C Polar embedded butyl Yes 00 0-7. C8 Octyl Yes 00 0 8-7. C8 Octadecyl Yes 00 0 9-7. Diol Propyldiol No 00 0 9-7. The Inertsil WP00 series of columns has been developed for the analysis and purification of proteins and peptides. It currently consists of five phases, which are available in column dimensions ranging from capillary to preparative. Inertsil WP00-C8 and C8 show high separation efficiency for peptides and proteins of medium molecular size (recommended <,000 Da for C8 phase and <0,000 Da for C8 phase). Inertsil WP00-C can elute hydrophobic proteins >,000 Da with excellent peak shape due to the shielding effects of embedded polar groups. Figure 8 shows the separation of a polypeptide mixture on Inertsil WP00-C8. Please contact Hichrom for ordering information for Inertsil wide pore phase columns. Column: Inertsil WP00-C8 (0 x.6 mm) Eluent: A: H O (0.% TFA) B: CH CN-H O (90:0) (0.% TFA) Gradient: A:B 80:0 to 0:60 over mins Temperature: 0 C. Ribonuclease. Insulin. Cytochrome. Lysozyme. BSA 6. Trypsin Inhibitor from Soybean 7. Ovalbumin 6 7 0 0 0 Mins Figure 8. Analysis of polypeptide mixture Capillary and Nano Columns Excellent efficiency and column-to-column reproducibility Easy installation Easy coupling with MS GL Sciences produce a range of capillary and nano columns for high sensitivity trace analyses. These columns are available for InertSustain, Inertsil Series, Inertsil Series and Inertsil wide pore materials as well as Titansphere products, which are designed specifically for applications involving phosphopeptide extractions (see page 8). The Capillary EX columns are available in 0., 0. and 0.7mm i.d. and 0 or 0mm lengths. These columns are packed using glass-lined stainless steel tubing of / o.d. and come with stainless steel end fittings. Capillary EX-Nano columns are available with 0.0, 0.07, 0. and 0.mm i.d. with lengths of 0, 0 and 0mm. These columns are packed using fused silica tubing and inserted into / 6 o.d. PEEK tubing for protection, with stainless steel end fittings. Please contact Hichrom for ordering information for Inertsil Capillary EX and Capillary EX-Nano columns. www.hichrom.co.uk sales@hichrom.co.uk Tel: 08 90 660
Please contact Hichrom for current prices. Tel: + (0)8 90 660 Email: sales@hichrom.co.uk GL Sciences Inertsil Series and Inertsil ODS Spherical porous silica Excellent for acidic and basic molecules Well referenced in the scientific literature Inertsil Series and Inertsil ODS Phases Functional Group Endcapped Particle Size (μm) Pore Size (Å) Surface Area (m /g) Carbon Load (%) ODS- Octadecyl Yes 0 0 8. C8 Octyl Yes 0 0 0. C Butyl Yes 0 0 7. Phenyl Phenyl Yes 0 0 0 ODS* Octadecyl Yes 00 0 * From original Inertsil series The well established Inertsil Series phases are based on high purity silica which is robust and shows high reproducibility from batch-to-batch and column-to-column. Ordering Information Hichrom Manufactured Columns Microbore (.0mm i.d.) Columns Column Dimensions (mm) Guard Cartridges 0 x.0 00 x.0 0 x.0 0 x.0 (For.0mm i.d. Columns) ODS- INODS-0M 7 INODS-00M 90 INODS-0M 0 INODS-0M INODS-0CE 0 (/pk) C INC-0M INC-00M INC-0M 6 INC-0M 9 INC-0CE 0 (/pk) Phenyl INP-0M 8 INP-00M 00 INP-0M INP-0M INP-0CE 0 (/pk) Microbore (.mm i.d.) Columns Column Dimensions (mm) Guard Cartridges 0 x. 00 x. 0 x. 0 x. (For.mm i.d. Columns) ODS- INODS-0AM 0 INODS-00AM 6 INODS-0AM 80 INODS-0AM 0 INODS-0CM 0 (/pk) C INC-0AM 0 INC-00AM INC-0AM 0 INC-0AM 70 INC-0CM 0 (/pk) Phenyl INP-0AM 60 INP-00AM 7 INP-0AM 90 INP-0AM 0 INP-0CM 0 (/pk) Medium Bore (.mm i.d.) Columns Column Dimensions (mm) 0 x. 00 x. 0 x. 0 x. Guard Cartridges (For.mm i.d. Columns) ODS- INODS-0AS INODS-00AS 0 INODS-0AS INODS-0AS 8 INODS-0C 0 (/pk) C INC-0AS 8 INC-00AS 00 INC-0AS INC-0AS INC-0C 0 (/pk) Phenyl INP-0AS INP-00AS 0 INP-0AS 6 INP-0AS 9 INP-0C 0 (/pk) Analytical (.0mm i.d.) Columns For free technical advice and support email technical@hichrom.co.uk Column Dimensions (mm) Guard Cartridges 0 x.0 00 x.0 0 x.0 0 x.0 (For.0mm i.d. Columns) ODS- INODS-0AF INODS-00AF 0 INODS-0AF INODS-0AF 8 INODS-0C 0 (/pk) C INC-0AF 8 INC-00AF 00 INC-0AF INC-0AF INC-0C 0 (/pk) Phenyl INP-0AF INP-00AF 0 INP-0AF 6 INP-0AF 9 INP-0C 0 (/pk) Analytical (.6mm i.d.) Columns Column Dimensions (mm) Guard Cartridges 0 x.6 00 x.6 0 x.6 0 x.6 (For.6mm i.d. Columns) ODS- INODS-0A INODS-00A 0 INODS-0A INODS-0A 8 INODS-0C 0 (/pk) C INC-0A 8 INC-00A 00 INC-0A INC-0A INC-0C 0 (/pk) Phenyl INP-0A INP-00A 0 INP-0A 6 INP-0A 9 INP-0C 0 (/pk) Other column dimensions available please enquire Use with free-standing holder HI-6 ( ) and column coupler HI-08 ( 6) see p. Starter kits ( ) also available see p. Semi-Preparative and Preparative (7.7.mm i.d.) Columns Please contact Hichrom for further details of 7.7.mm i.d. Inertsil columns. 6
Please contact Hichrom for current prices. Tel: + (0)8 90 660 Email: sales@hichrom.co.uk GL Sciences Ordering Information GL Sciences Manufactured Columns (Inertsil Series and Inertsil ODS) Microbore (.mm i.d.) Columns Column Dimensions (mm) Inertsil μm Phase 0 x. 0 x. Guard Cartridges (/pk) (For.mm i.d. Columns) 7 8 8 ODS- 00-0 00-0 00-9 9 0 8 C8 00-0 00-0 00-96 Phenyl 00-0 00-0 00-97 8 7 8 C 00-0 00-0 00-98 8 7 8 ODS 00-0 00-0 00-9 Medium Bore (.0mm i.d.) Columns Inertsil μm Phase Column Dimensions (mm) Guard Cartridges (/pk) 0 x.0 0 x.0 (For.0mm i.d. Columns) 7 8 6 ODS- 00-0 00-06 00-9 9 0 6 C8 00-0 00-06 00-96 Phenyl 00-0 00-06 00-97 8 7 6 C 00-0 00-06 00-98 8 7 6 ODS 00-0 00-06 00-9 Analytical (.0 and.6mm i.d.) Columns Inertsil μm Phase Column Dimensions (mm) Guard Cartridges (/pk) 0 x.0 0 x.0 0 x.6 0 x.6 (For.0 and.6mm i.d. Columns) 7 8 7 8 6 ODS- 00-0 00-07 00-0 00-08 00-90 9 0 9 0 6 C8 00-0 00-07 00-0 00-08 00-906 Phenyl 00-0 00-07 00-0 00-08 00-907 8 7 8 7 6 C 00-0 00-07 00-0 00-08 00-908 8 7 8 7 6 ODS 00-0 00-07 00-0 00-08 00-90 Other column dimensions available Use with free-standing holder 00-0800 ( 97) www.hichrom.co.uk sales@hichrom.co.uk Tel: 08 90 660 7