Kromasil Eternity Designed for long life For use in HPLC and UHPLC Kromasil
The long-lasting phase for demanding applications Imagine working under virtually no ph restrictions during method development. That s exactly what Kromasil Eternity allows you to. Kromasil Eternity is our latest innovation for separation and purification of compounds under reversed phase HPLC and UHPLC. Since many pharmaceutical compounds are ionizable, the broad ph user window from to allows you more flexibility in altering retention time during method development. However, wide ph variations have a negative impact on column lifetime. That s why Kromasil Eternity is based on a patent pending grafting technology (see the three-step description), securing a long-lasting product even under tough ph conditions as well as at high temperatures. This folder gives an overview of how you benefit. To learn more, visit www.kromasil.com or contact us directly. Modified silica Long-lasting columns Better economy Small particles (.5 µm) High efficiency Faster analyses Modified silica ph to Easier method development
Bare silica Produced in-house at Akzoobel/Separation Products, ensuring high mechanical stability and efficiency. Pore size: Å. Modified silica The silica is bonded with an organosilane layer. Under specific proprietary conditions, the organosilane layer penetrates the silica, which results in a merged organic/inorganic interfacial gradient. The pores are virtually returned to their original size resulting in a surface presenting both inorganic (-H) and organic (-R) silicic acid moieties. This process step is what gives Kromasil Eternity its extreme chemical stability extending the ph range and column lifetime. Finished product Finally, the product is functionalized with C8 followed by a proprietary endcapping process.
The product program,, Product assortment.5 µm Eternity 5 µm Eternity. x 5 mm Eternity-.5-C8. x 5 Eternity-5-C8. x 5. x mm Eternity-.5-C8. x. x 5 mm Eternity-5-C8. x 5.6 x 5 mm Eternity-.5-C8.6 x 5 Eternity-5-C8.6 x 5.6 x mm Eternity-.5-C8.6 x Eternity-5-C8.6 x.6 x 5 mm Eternity-5-C8.6 x 5.6 x 5 mm Eternity-5-C8.6 x 5 x 5 mm Eternity-5-C8 x 5 x 5 mm Eternity-5-C8 x 5 x 5 mm Eternity-5-C8 x 5. x 5 mm Eternity-5-C8. x 5. x 5 mm Eternity-5-C8. x 5. x 5 mm Eternity-5-C8. x 5 x 5 mm Eternity-5-C8 x 5 x 5 mm Eternity-5-C8 x5 x 5 mm Eternity-5-C8 x 5 ) ther column dimensions available upon request. ) Also available in columns for UHPLC use. ) Guard columns are available for each column dimension Analytical scale Semi preparative scale Product characteristics Ligand: C8 Pore size: Å Surface area: m /g Carbon load: % Endcapping: Proprietary ph range: - USP: L Application tests Long term ph stability test Columns: Kromasil Eternity-5-C8.6 5 mm Mobile phase A: mm ammonium bicarbonate, ph.5/acetonitrile (9/) Mobile phase B: mm ammonium bicarbonate, ph.5/acetonitrile (/9) Flow rate: ml/min Temperature: 5 C Gradient min % A min % B 5 min % B 6 min % A min % A Test amitriptyline: mm ammonium bicarbonate, ph.5/acetonitrile (/7) Test prednisolone: mm ammonium bicarbonate, ph.5/acetonitrile (7/) Test cycle: 6 gradient + tests = 7 min/cycle Percentage of initial efficiency Percentage of initial efficiency [%] 8 6 Kromasil Eternity Waters XBridge 6 8 [%] Prednisolone 8 6 Amitriptyline H H H Kromasil Eternity Waters XBridge 6 8 [min.] [min.]
ph variation to control selectivity Column: Mobile phase: Gradient: Flow rate: Temperature: 5 C Detection: UV 5 nm = uracil = procaine H H Kromasil Eternity-.5-C8.6 x 5 mm acetonitrile/ mm sodium phosphate ph., 7. and., respectively -.5 min: %, 5.5 min: 5% acetonitrile.5 ml/min H = fenuron = -nitrobenzoic acid H H + - ph. ph 7. ph. 5 6 [min.] Time efficiency Mobile phase: acetonitrile/water/formic acid (5/75/.) Substances: Mix of sulfa-drugs = uracil = sulphathiazole = sulphamerazin = sulphamethoxazole Temperature: 5 C Detection: UV 5 nm Save even more time with shorter column and increased flow rate Rs = Save time without resolution loss by decreasing particle size Rs = 5..6.9. [min.] Stationary phase: Kromasil Eternity-.5-C8 Column size:.6 x 5 mm Flow rate:.7 ml/min Rs = 6 5 5 [min.] Stationary phase: Kromasil Eternity-5-C8 Column size:.6 x 5 mm Flow rate:. ml/min.5.5 [min.] Stationary phase: Kromasil Eternity-.5-C8 Column size:.6 x mm Flow rate:.6 ml/min Run at / of original analysis time Scale-up or Scale-down Sample: Mix of β-blockers = uracil = atenolol = pindolol = metoprolol 5 = propanolol 6 = alprenolol Mobile phase: acetonitrile/5 mm triethylamine acetate, ph (/6) Flow rate:. ml/min and 9. ml/min for.6 and. mm i.d. columns, respectively Temperature: C Detection: UV nm Column length: 5 mm 5 [min.] 5 6 5 µm,. x 5 mm 5 µm,.6 x 5 mm.5 µm,.6 x 5 mm