Grace HPLC Column Solutions Address Today s Separation Challenges

& accessories Grace HPLC Column Solutions Address Today s Separation Challenges This catalog features Grace s leading HPLC column technologies. You will find innovative chemistries, unsurpassed reliability, and hardware configurations that optimize your method requirements for speed, sensitivity, resolution or sample loading. Discover how our select range of small molecule columns, biomolecule-based offerings and ion chromatography technologies address your toughest separation challenges. Grace Featured Column Families: Unique chemistries in sub to μm particles maximize performance and speed for UHPLC and HPLC. Everest High capacity for complex peptide mapping and proteomics applications. Premium quality, exceptionally stable columns, without phase bleed. ProZap High-speed protein and peptide separations. Prevail Compatible with % aqueous to % organic mobile phases. Vydac TP Popular, industry-standard bioseparations columns. GraceSmart High quality HPLC columns at an exceptional value. Alltech IC Wide variety for the separation of organic and inorganic anions and cations. Vydac MS Unique selectivity and recovery for protein isolation and characterization. Grace Expertise and Product Ownership Grace has been manufacturing silica and bonding it with unique chemistries for more than three decades. Our combined media production, column packing, and subμm particle expertise uniquely positions us to solve the challenges of modern chromatography laboratories. Partner with Grace and be assured our consistent, reliable chromatography products adhere to strict quality standards. Additional Column Brands Available While this catalog focuses on Grace leading HPLC columns, other popular brands are still available. Visit our website for a comprehensive listing. Alltima Adsorbosphere Genesis Grom Sil Certified in ISO 9, and 8 procedures FDA CFR 8 for medical devices Full traceability from base silica to final product Extensive process control to ensure highest reproducibility Experienced global technical support and sales teams Allsphere Apex Apollo Brava Denali Econosphere Grom Sapphire Macrosphere Platinum ProSphere Vydac SP

& accessories HPLC Column Hardware Formats HPLC Column Hardware Formats i.d. Format UHPLC Capillary.... Length System Compatibility Description Advantage Brand Availability UHPLC system, LC/MS Capillary systems, LC/MS Ultra-low dead volume hardware, pressure rated to 8,psig, allows X faster linear velocities. Highly specialized columns packed in small diameter fused silica tubing. Packed with subμm media, this hardware delivers the fastest separations with maximum efficiency. Extremely low sample dispersion offers best sensitivity. High-sensitivity and samplelimited applications. Vydac MS Everest Expedite.. Low volume, highthroughput (HTP) systems Extremely short, low-volume columns that prevent sample diffusion. High-throughput and rapid resolution LC/MS applications packed with highly efficient.μm media. Prevail ProZap Microbore. Standard HPLC optimized for low volume.mm diameter analytical columns requiring low volume systems. Low solvent consumption and improved sensitivity compared to traditional.mm columns. Prevail GraceSmart Vydac MS Everest IC Solvent Reducer Standard HPLC mm diameter analytical columns suitable for use on conventional HPLC. % solvent savings compared to traditional.mm columns. No need to optimize system for low volume. Prevail Vydac MS Vydac TP Analytical. Standard HPLC Conventional.mm i.d. with industry standard port configurations. Most common, traditional hardware format. Prevail GraceSmart Vydac MS Everest Vydac TP IC Rocket Standard HPLC Large mm i.d. balances column volume with conventional LC system volume, and allows faster mobile phase flow rates. Ultra-fast separations on conventional HPLC systems. Unique hardware geometry reduces peak broadening and delivers excellent peak shapes. ml/min max flow rate reduces analysis time up to 8% compared to traditional analytical columns. Prevail Preparative Preparative LC Large i.d. increases loading. Packed under high pressure for high efficiency LC purifications. Vydac MS Vydac TP Capillary Guard.. UHPLC Guard Guard system with reusable holder and disposable cartridges. Protects capillary columns and prolongs life. Integral and stand-alone options. Protects UHPLC column investment, and prolongs life. Vydac MS Everest All-Guard... Guard system with reusable holder and disposable cartridges. Protects analytical and microbore columns and prolongs life. Prevail Vydac MS Everest Vydac TP www.discoverysciences.com

& accessories HPLC Phase Specifications and Usage Columns for Small Molecules Brand Phase C8 HighLoad Pore Size (Å) Surface Area (m /g) Carbon Load (%) Endcapped Chromatographic Properties Application/Benefit Yes Ultra high-purity silica, fully bonded. C8 Basic Proprietary Ultra-high purity silica. Controlled silica surface exposure gives dual mode separation with polar and nonpolar analytes. C8 Classic Yes Lower carbon load. Slight silica exposure. C8 Polar No High silica exposure, low carbon load. Uniform coverage of inert vicinal silanols. HILIC No Polar phase with shorter equilibration times. Shipped in ACN/Water. Silica No Traditional normal phase for use in % organic mobile phases. General purpose for broad range compounds, classic selectivity, high-capacity for hydrophobic compounds. Alternate reversed-phase selectivity. High polar retention especially with compounds having two or more polar groups. Excellent sensitivity and peak shape for basic compounds, without the need for acidified mobile phases. Reversed-phase separations with reduced bonding optimized for speed. Some additional polar retention. Unique polar selectivity. Low carbon load gives fastest reversed-phase elution times while retaining polar compounds longer. Peak reversal compared to reversed-phase. Ideal for very polar compounds with high organic mobile phases for improved sensitivity by MS. For isomeric separation of non-aqueous compatible compounds by absorption chromatography. C8 9 Yes Classic reversed-phase retention and selectivity. Routine applications. C8 EPS 9 Yes Greater retention and enhanced Reversed-phase applications where C8 is too peak symmetry for polar retentive. compounds. Alternate selectivity to traditional reversed-phases. C8 Hi-Load Yes Highest carbon load for superior High resolution for complex samples. retention and loadability. C8 AQ Yes % water wettable. Applications requiring high aqueous mobile phases. C8 Amide 9 Yes Polar-embedded phase with extremely low bleed. Compatible with microbore. C8 9 8 Yes Lower capacity compared to C8 phases. Cyano 9 Yes Extremely stable, long life and reproducible. Basic compounds in neutral to alkaline ph, MS applications. Reversed-phase applications where C8 is too retentive. Ideal for basic drug analysis. Silica No Highly polar phase. General purpose normal phase applications. L HILIC No Hydrophilic interaction Very polar analytes that cannot be retained by L chromatography uses small amounts of water for increased sensitivity with microbore applications. reversed-phase. Prevail C8 Select Yes Stable in % aqueous to % organic mobile phases. Same benefits as Prevail C8, but less retentive for polar compounds. L C8 Yes Stable in % aqueous to % organic mobile phases. Flexibility to switch between varied mobile phase conditions to suit a variety of applications. Excellent sensitivity for microbore applications. C8 8 Yes Stable C8 phase. Use for highly hydrophobic compounds that retain too strongly on C8. L Phenyl Yes Lowest hydrophobic capacity. Selective for aromatic compounds in a variety of L mobile phase conditions. Cyano Yes General purpose cyano suitable Rugged normal phase applications. L for normal or reversed-phase use. Amino No Stable in % aqueous to Use for carbohydrates or as a weak anion L8 % organic mobile phases. exchanger. Silica No Highly polar phase. General purpose normal phase applications. L Organic Acid Yes Highly efficient silica-based, acid-stable. Separate common organic acids with unsurpassed resolution, speed and sensitivity. Lower cost than polymeric columns. Carbohydrate No Extremely stable hybrid phase. Excellent for mono- and oligosaccharides and ES sugar alcohols. GraceSmart C8 Yes High purity silica with monomeric C8 bonding offers classical reversed-phase selectivity. Excellent value for general purpose, routine applications. USP L-code L L L L L L L L L L L L L L L

& accessories Columns for Large Molecules / Life Science Pore Surface Carbon Brand Phase Size (Å) Area (m /g) Load (%) Endcapped Chromatographic Properties Application/Benefit Vydac MS 8MS C8 8 Yes Polymeric bonding, highest hydrophobic Use for simple enzymatic digests (< proteins) interaction and unique geometric selectivity. or biomolecules K MW. 8MS C8 Yes Monomeric bonding offers increased peptide interaction and generally yields higher peak counts. 8MS C8 Yes Lower hydrophobicity is better for larger biomolecules. MS C Yes Lower capacity than C8 or C8, suitable for hydrophobic proteins or when minimal organic is desired. 9MS Yes Lowest capacity, aromatic functional Diphenyl groups. Everest 8EV C8 Yes Maximum surface coverage for highest resolution of complex samples. Use for same applications as 8MS, but offers different C8 selectivity. Ideal for biomolecules K MW. Ideal for biomolecules >K MW, undenatured intact proteins, antibodies, oligonucleotides, human growth hormone. Highly selective for proteins with aromatic side chains. Complex enzymatic digests (> proteins). ProZap C8 9 Yes Subμm, Å wide pore. Ideal for fast, intact protein or peptide analysis. L Vydac TP 8TP C8 8 Yes First generation polymeric C8 media with unique selectivity. Small polypeptides K MW, enzymatic digest L fragments, natural and synthetic peptides, multi-ring compounds. 8TP C8 Yes First generation monomeric C8 media. Use for same applications at 8TP, but offers L different C8 selectivity. 8TP C8 Yes Less hydrophobic than C8TP media. Polypeptides K MW. L TP C Yes First generation C media. Glycoproteins, hemoglobin variants, histones, insulin variants, membrane proteins. L 9TP Diphenyl Yes Lowest capacity first generation diphenyl media. Reversed-Phase Column Comparisons Polypeptides with aromatic side chains, large hydrophobic proteins, membrane-spanning peptides, lipid peptides, fusion proteins from inclusion bodies. USP L-code L Based on the widely accepted work and test procedures of Drs Lloyd Snyder and John Dolan,,, Grace developed this tool to show relative selectivity comparisons for commercially available columns. This graph plots the indicators for hydrophobicity versus the cation exchange capacity at ph. Hydrophobicity is often the primary analyte interaction and indicates overall capacity. The cation exchange capacity measures secondary interactions that take place and can greatly influence the retention of polar analytes. L L L L L L C8 Polar C8 Basic Acquity HSS C8 SB Extended Polar Retention Prevail Amide Prevail C8 Universally Retentive Increasing Polar Retention C8 Amide C8 EPS Synergi Polar-RP Zorbax SB-AQ To find columns that will produce alternate separation profiles, choose points that are in opposite quadrants. Prevail Select C8 GraceSmart C8 C8 Classic Hypersil GOLD aq Hypersil GOLD Acquity BEH Shield RP-8 Kinetex XB-C8 Atlantis T Gemini C8 Acquity HSS T Kinetex C8 Ascentis ACE C8 RP-Amide XTerra MS C8 Luna C8 Zorbax SB C8 To find columns with similar selectivity, choose points that are close together. Inertsil ODS- Zorbax Rx-8 Hypersil ODS- Hypersil BDS C8 Pinnacle DB C8 C8 C8 HighLoad XBridge C8 Symmetry C8 Zorbax Eclipse XDB-C8 Acquity HSS C8 Zorbax Extend C8 Kromasil C8 XTerra C8 RP C8 High Load Low Capacity Increasing Hydrophobic Retention Traditional RP Selectivity Data courtesy of Lloyd Snyder and John Dolan. The Hydrophobic-subtraction Model of Reversed-phase Column Selectivity, L.R. Snyder, J.W. Dolan and P.W. Carr, J. Chromatogr. A, (). A New Look at the Selectivity of Reversed-phase HPLC Columns, L.R. Snyder, J.W. Dolan and P.W. Carr, Anal. Chem., 9 (). www.discoverysciences.com

& accessories Application-Based Column Selection Use this chart to identify a suitable column for your application. Traditional choice columns are well matched to the application. Alternative choice columns can provide different selectivity when the traditional choice column does not work. Legend: Traditional Choice Application-Based Column Selection Pharmaceutical Neutraceutical / Natural Products Food & Beverage Environmental Biopharmaceutical/ Life Science Acidic Column C8 HighLoad C8 Basic C8 Classic C8 Polar Silica C8 C8 EPS C8 Hi-Load C8 AQ C8 Amide C8 Cyano Prevail C8 Select Prevail C8 Prevail Phenyl Prevail Organic Acid Prevail Carbohydrate ES GraceSmart C8 Vydac 8MS C8 Vydac 8MS C8 Vydac 8MS C8 Vydac MS C Vydac 9MS Diphenyl Everest 8EV C8 ProZap C8 Vydac 8TP C8 Vydac TP C *For UHPLC applications. Basic Neutral Herbal Medicines Vitamins Dietary Supplement Carbohydrates Lipids Organic Acids Food Safety Agricultural Chemicals Industrial/Aromatics Simple Enzymatic Digests (< proteins) Complex Enzymatic Digests (> proteins) Proteins, Peptides, Biomolecules K MW * * * * * * Alternative Choice K MW >K MW Undenatured, Intact Proteins Antibodies Oligonucleotides

& accessories Empty HPLC Hardware Stainless Steel Analytical Column Hardware Stainless Steel Preparative Column Hardware Threaded Column Tube Frit/Insert Endfitting Column Tube Continuously Adjustable Piston/Frit Endfitting Stainless steel analytical columns come complete with the threaded tube, two μm industry standard frit/inserts, and two stainless steel endfitting nuts. Optional.μm porosity frit/ inserts are also available. Adapters and sealing rings are available for slurry packing. The packing adapter comes with a /" precolumn tube to connect to /" slurry reservoirs. Stainless Steel Analytical Column Hardware Specifications Material: Stainless Steel Max. Temperature: ºC Max. Pressure:,psig Thread Type: - UNF (CPI Standard Ports) Typical Use: Analytical HPLC Columns Stainless Steel Analytical Hardware and Accessories Description.mm i.d..mm i.d. Complete Columns mm mm mm mm 8 mm 8 Replacement Parts Endfitting (ea) Frit/Insert, μm (/pk) 8 Frit/Insert,.μm (/pk) The easy-to-use preparative format has threaded endfittings and a continuously adjustable piston/frit. The piston/frit extends column life by compressing the packed bed to remove voids. Stainless Steel Preparative Column Hardware Specifications Material: Stainless Steel, PEEK Max. Temperature: ºC Max. Pressure: 8psig Thread Type: - UNF (CPI Standard Ports) Typical Use: Prep HPLC Columns Stainless Steel Preparative Hardware and Accessories i.d. Length Qty. Complete Columns mm mm ea 9 mm mm ea 9 mm mm ea 9 mm mm ea 9 mm mm ea 9 mm mm ea 9 mm mm ea 9 mm mm ea 9 Replacement Piston/Frit, μm mm ea 9 mm ea 9 mm ea 9 Stainless Steel Frits Stainless Steel Frit Specifications Material: Stainless Steel Max. Temperature: ºC Max. Pressure:,psig Typical Use: Column Inlet/Outlet Stainless Steel Frits Frit Diameter Total Diameter Thickness Porosity /pk /" (.mm) /" (.mm)." (.9mm).μm 8 /" (.mm) /"(.mm)." (.9mm).μm /" (.mm) /" (.mm)." (.9mm).μm /8" (9.mm) /8" (9.mm)." (.9mm).μm Encased Metal-Free Frits Encased Metal-Free PEEK Frit Specifications Material: PEEK Max. Temperature: ºC Max. Pressure:,psig Typical Use: Column Inlet/Outlet Encased Metal-Free PEEK Frits Frit Diameter Total Diameter Thickness Porosity /pk PEEK Frits with PEEK Ring.8" (.mm) /" (.mm)." (.9mm) μm 8.8" (.mm) /" (.mm)." (.9mm) μm 9 technical assistance Contact Tech Support: Email: discoverysciences@grace.com Online: www.discoverysciences.com www.discoverysciences.com

& accessories Columns One Platform for UHPLC, HPLC and PREP Separate complex samples with excellent resolution with high performance columns. They are available with.,, and μm particle sizes and six different phase selectivities spanning the full polarity spectrum. The same base silica and bonding chemistries are used across all particle sizes therefore offering simple method transfer between UHPLC, HPLC and preparative systems. Phase Specifications Pore Size (Å) Surface Area (m /g) Carbon Load (%) Endcapped Chromatographic Properties Phase C8 HighLoad Yes Ultra-high purity silica, fully bonded. C8 Basic Proprietary Ultra-high purity silica. Controlled silica surface exposure gives dual mode separation with polar and non-polar analytes. C8 Classic Yes Lower carbon load. Slight silica exposure. C8 Polar No High silica exposure, low carbon load. Uniform coverage of inert vicinal silanols. HILIC No Polar phase with shorter equilibration times. Shipped in ACN/Water. Silica No Traditional normal phase for use in % organic mobile phases. Application/ Benefit General purpose for broad range compounds, classic selectivity, high-capacity for hydrophobic compounds. Alternate reversed-phase selectivity. High polar retention especially with compounds having two or more polar groups. Excellent sensitivity and peak shape for basic compounds, without the need for acidified mobile phases. Reversed-phase separations with reduced bonding optimized for speed. Some additional polar retention. Unique polar selectivity. Low carbon load gives fastest reversed-phase elution times while retaining polar compounds longer. Peak reversal compared to reversed-phase. Ideal for very polar compounds with high organic mobile phases for improved sensitivity by MS. For isomeric separation of non-aqueous compatible compounds by absorption chromatography. USP L-code L L L L L L Simple Method Transfer Optimizing and transferring methods between HPLC, UHPLC and preparative systems is not a simple and intuitive task. However, when the same base silica and bonding is available in sub-,,, and μm particles and in a variety of column formats, methods can be more easily transferred across system types and between laboratories, improving efficiency and productivity. Identical Selectivity Across All Particle Sizes.....8.... Snyder-Dolan-Carr Coefficients for C8 Basic Phase.μm.μm.μm μm H S A B C.8 C References:. "The "Hydrophobic-subtraction" Model of Reversed-phase Column Selectivity", L.R. Snyder, J.W. Dolan and P.W. Carr, J. Chromatogr. A, () -.. "A New Look at the Selectivity of Reversed-phase HPLC Columns", L.R. Snyder, J.W. Dolan and P.W. Carr, Anal. Chem., 9 () -. Method Transfer. Uracil. Benzyl alcoho. Benzonitrile. Anisole. Ethyl benzene. Hexaphenone. x mm, μm,.ml/min.. x mm, μm,.ml/min. x mm, μm,.ml/min.. x mm,.μm,.ml/min. x mm, μm,.ml/min. 8 9 min. Column: Mobile Phase: C8, HighLoad, Dimensions noted A: mm phosphate buffer, ph B: Acetonitrile Gradient: Time: %B: 9 9 8

& accessories Broad Selectivity Range Four reversed-phase column chemistries offer complementary selectivity. The unique C8 Polar and Basic phases retain polar compounds while the C8 HighLoad has a more traditional reversed-phase selectivity. Using phases with orthogonal selectivity gives confidence that if one phase does not produce the separation the other will. Reversed-Phases Span the Full Polarity Range VisionHT C8 Polar Phase VisionHT VisionHT C8 Basic C8 Column Basic Strongest retention for polar compounds Key columns Commercially available columns Increasing Polar Retention VisionHT C8 Classic Column Classic retention for hydrophobic compounds VisionHT C8 HighLoad High-Load Column Increasing Hydrophobic Retention References:. "The "Hydrophobic-subtraction" Model of Reversed-phase Column Selectivity", L.R. Snyder, J.W. Dolan and P.W. Carr, J. Chromatogr. A, () -.. "A New Look at the Selectivity of Reversed-phase HPLC Columns", L.R. Snyder, J.W. Dolan and P.W. Carr, Anal. Chem., 9 () -.....8.... Different Selectivities of Phases, Neutral Compounds. Benzonitrile. Anisole.,-Diphenylhydantion. -Methyl salicylaldehyde Classic HighLoad Basic Polar Column: Various Phases,.μm,. x mm Mobile Phase: A: ACN B: Water Gradient: Time: %B: 9 Flow Rate:.mL/min Detector: UV nm 8 9 min. +..... Different Selectivities of Phases, Polar Compounds. Acetaminophen. Caffeine. N,N'-Diethylacetamide. Benzonitrile 8 9 min. Column: Various Phases,.μm,. x mm Mobile Phase: A: ACN B: Water Gradient: Time: %B: 9 Flow Rate:.mL/min Detector: UV nm Classic HighLoad Polar Basic www.discoverysciences.com 9

8 8 8 8 8 9 8 998 8 9 8 & accessories Consistent and Reliable Performance Ultra-high mechanical strength silica and robust bonding chemistry minimize variations in capacity and selectivity. Media consistency combined with reproducible column packing methods delivers reliable performance and long column lifetime. Reproducible Particle Technology Surface Area Mean = RSD =.% Pore Volume Mean =. RSD =.% Pore Diameter Mean = 99. RSD =.% Consistent Column-to-Column Performance Biphenyl Nitrobenzene Amitriptyline Cinnamic Acid Toluene. Surface Area (m /g) Pore Diameter (Å)... Pore Volume (cc/g) Capacity Factor..... 9 9 Consecutive Batches. Consecutive Batches silica's surface area, pore volume, and pore diameter are highly reproducible. media and advance packing methods produce columns with highly consistent capacity and selectivity. Constant Performance after Routine Exposure to,psig Pressure min. UHPLC columns rated to,psig pressure limit. Uracil (.mg/ml). Phenol (.mg/ml). N,N-Diethyl-m-toluamide (.mg/ml). Toluene (.mg/ml) INITIAL min. FINAL (After, column volumes at,psig) Plates/Meter High-Pressure Stability Column Comparison, x mm* Column Column Column Column Column Column Column Competitor Sub μm Column Column Volumes Competitor Sub μm Column Column: Mobile Phase: Flow Rate: Detector: C8 Polar,.μm,. x mm ( 9) (8:) Acetonitrile:Water.mL/min UV at nm *After exposure to,psig Before and after chromatograms show a consistent level of performance after exposure to,psig pressures for, column volumes. Competitive columns lose performance over time under high-pressure conditions while columns remain stable.

& accessories Method Development Ultra MD Kits Experience Broad Selectivity Range of the Phases Ultra MD kits include phases with orthogonal selectivity to enable fast and efficient method development for the separation of very polar to very hydrophobic compounds. Column Ultra MD Kits Description Phases Dimensions Ultra MD Kit C8 Classic, C8 Polar, x mm 9 C8 HighLoad, C8 Basic Ultra MD Kit C8 Classic, C8 Polar, x mm 9 C8 HighLoad, C8 Basic Ultra MD Kit C8 Classic, C8 Polar, C8 HighLoad, C8 Basic x mm 9 Guard Columns Protect Your Column to Minimize Downtime and Reduce Cost Couple the zero-dead volume integral guard directly to any column, with no loss in efficiency. Columns i.d. Length Particle Size (μm) C8 HighLoad C8 Basic C8 Classic C8 Polar HILIC Silica Format UHPLC. 9 9 9 9. 9 99 9 9. 9 9 9 9 9. 9 9 98 9 9. 9 9 98 9. 9 9 9 9. 98 9 9 99 9. 99 99 9 9 9. 88 88 88 88 9 Expedite.. 98 9.. 98 8 Microbore. 98 98 9. 98 9. 99 9 9 Solvent 98 9 Reducer 9 8 9 998 89 9 999 9 99 88 Analytical. 9 99 8. 9 99 9. 9 99 8 8. 9 99 8. 9 9 8 Rocket. 98 98 9. 98 99 9 Preparative 98 8 988 9 989 8 99 8 99 8 8 Capillary. 9 9 9 9 9 9 Guard*. 8 9 9 9 9 9 All-Guard **.. 9 98 9 99 9 9.. 9 9 9 9 9 9 *Requires Capillary holder, 8. **Requires All-Guard Holder, 8. more info For detailed information on column formats, see page. technical assistance Contact Tech Support: Email: discoverysciences@grace.com Online: www.discoverysciences.com www.discoverysciences.com

& accessories Columns Stability, Purity and Performance columns combine our best phase chemistries with high-purity silica. The result is one product family with the selectivity and performance needed to help overcome your most challenging separation needs. Phase Specifications Phase Pore Size (Å) Surface Area (m /g) Carbon Load (%) Endcapped Chromatographic Properties C8 9 Yes Classic reversed-phase retention and selectivity. Application/ Benefit Routine applications. C8 EPS 9 Yes Greater retention and enhanced peak Reversed-phase applications symmetry for polar compounds. Alternate where C8 is too retentive. selectivity to traditional reversed-phases. C8 Hi-Load Yes Highest carbon load for superior retention High resolution for complex and loadability. samples. C8 AQ Yes % water wettable. Applications requiring high aqueous mobile phases. C8 Amide 9 Yes Polar-embedded phase with extremely Basic compounds in neutral to low bleed. Compatible with microbore. alkaline ph, MS applications. C8 9 8 Yes Lower capacity compared to C8 phases. Reversed-phase applications where C8 is too retentive. Cyano 9 Yes Extremely stable, long life and Ideal for basic drug analysis. reproducible. Silica No Highly polar phase. General purpose normal phase applications. HILIC No Hydrophilic interaction chromatography uses small amounts of water for increased sensitivity with microbore applications. Very polar analytes that cannot be retained by reversed-phase. USP L-code L L L L L L L L L The Column Family Demonstrates Low Bleed columns are among the lowest-bleed columns available, making them ideal for microbore applications. The C8 amide polar-embedded phase and the cyano phase have significantly lower column bleed than competitive columns. Column Bleed by ESI-MS vs. Competitive Columns Cyano Much More Stable than Other Cyanos Relative Abundance Level (X ) 8 No Column.8 Alltech Column Competitor Columns Silica C8 C8 Hi-Load C8 MS..8.8.8 C8 MS.9 C8 Amide..8 C8 Amide. C8 Amide % Changes in Retention - - - - - - - - QC D Test: ph. ph. CN Brand A CN Brand B CN Brand C CN Brand D CN Brand E CN Brand F CN : HO/CHCN,.mL/min, UV at nm, μl DMix, Column. x mm, C ph. Stability: Flushed with HSO ph.:acetonitrile : at.ml/min for days ph. Stability: Flushed with mm KHPO ph.:acetonitrile : at.ml/min for days

& accessories Selectivity Options in One Product Family Capacity Factor k' Column Selectivity Comparisons 9 8 CN k' Toluene k' Butyl Benzoic Acid k' Amitriptyline C8 EPS C8 C8 Amide C8 C8 AQ C8 Hi-Load Acid, Base and Neutral Test Mix 8 Min. Column:. x mm Mobile Phase: mm KHPO, ph.:chcn (:) Flow Rate:.mL/min Detector: UV at nm. Uracil. Pyridine. Phenol. N,N-diethyl-m-toluamide. -Butyl Benzoic Acid. Toluene C8 C8 EPS C8 Hi-Load Columns i.d. Length Particle Size (μm) C8 C8AQ EPS C8 C8 Hi-Load C8 Amide C8 CN Silica HILIC Format Capillary. 9 9. 8. 8. 9 8 8 8. 8 9. 8 8 Expedite.. 8. 8. 8 8 89 88 8. 8 88. 8 88 89 88 Microbore. 8 88 8 8 8 8 8. 89 889 8 88 8 8. 8 88 8 888 8 8 8 888 8. 88 88 8 8 8 88 899 8. 88 88 88 8 8 88 Solvent 8 8 8 8 8 8 89 Reducer 8 8 88 8 8 8 8 8 89 8 88 8 Analytical. 88 88 88 88 889 88 88 8. 8 88 8 88 8 89 8. 88 888 8 88 8 8 88 88 8. 89 889 8 89 8 8 89 89 8. 88 88 8 898 8 8 88 898 8 Rocket. 88 8 88 8 8 889 89 8 88 88 89 8 8 8 89 8 Guard*.. 88 88 89 8 8 8 88 88 89. 8 8 8 8 8.. 88 88 8 88 8 88 88 88 *Requires All-Guard Holder, 8. more info For detailed information on column formats, see page. technical assistance Contact Tech Support: Email: discoverysciences@grace.com Online: www.discoverysciences.com www.discoverysciences.com

& accessories Prevail Columns Stable from % Organic to % Aqueous Prevail HPLC columns offer exceptional versatility for difficult separations. Traditional reversed-phase packings experience bonded phase collapse under highly aqueous (>9%) conditions. Prevail bonded phases remain fully extended in % aqueous and offer a high carbon load for exceptional retention at % organic. Retain both polar and nonpolar compounds simply by adjusting the mobile phase. Prevail Phase Specifications Phase Pore Size (Å) Surface Area (m /g) Carbon Load (%) Endcapped Chromatographic Properties Application/ Benefit C8 Select Yes Stable in % aqueous to Same benefits as Prevail C8, but slightly % organic mobile phases. more retentive. C8 Yes Stable in % aqueous to % organic mobile phases. Flexibility to switch between varied mobile phase conditions to suit a variety of applications. Excellent sensitivity for microbore applications. C8 8 Yes Stable C8 phase. Use for highly hydrophobic compounds that L retain too strongly on C8. Phenyl Yes Lowest hydrophobic capacity. Selective for aromatic compounds in a L variety of mobile phase conditions. Cyano Yes General purpose cyano Rugged normal phase applications. L suitable for normal or reversed-phase use. Amino No Stable in % aqueous to Use for carbohydrates or as a weak anion L8 % organic mobile phases. exchanger. Silica No Highly polar phase. General purpose normal phase applications. L Organic Acid Yes Highly efficient silica-based, acid-stable. Separate common organic acids with unsurpassed resolution, speed and sensitivity. Lower cost than polymeric columns. Carbohydrate ES No Extremely stable hybrid phase. Excellent for mono- and oligosaccharides and sugar alcohols. USP L-code L L Switch Between % Aqueous and % Organic Mobile Phases on the Same Column Water-Soluble Vitamins (% Aqueous) 9 Fat-Soluble Vitamins and Tocopherols (% Organic) 9 Water-Soluble Vitamins (% Aqueous) 9. Thiamine. Ascorbic Acid. Nicotinic Acid. Pyridoxine. Niacinamide. Pantothenic Acid. Vitamin A. -Tocopherol. -Tocopherol. -Tocopherol (Vitamin E). Vitamin D. Vitamin D. Vitamin K. Thiamine. Ascorbic Acid. Nicotinic Acid. Pyridoxine. Niacinamide. Pantothenic Acid wash with intermediate wash with intermediate 8 Min. 8 Min. 8 Min. Column: Mobile Phase: Flow Rate: Detector: Prevail C8, μm,. x mm mm KHPO ph.:chcn (9:).mL/min UV at nm Column: Prevail C8, μm,. x mm Mobile Phase: CHCN:CHOH (:) Flow Rate:.mL/min Detector: UV at nm Column: Mobile Phase: Flow Rate: Detector: Prevail C8, μm,. x mm mm KHPO ph.: CHCN (9:).mL/min UV at nm

& accessories Prevail Carbohydrate ES Columns Quiet Gradient Baselines Prevail Carbohydrate ES columns are excellent for isocratic separations with common detectors, but the column's real power shows with the ELSD and a solvent gradient. Enjoy the powerful selectivity, reduced run times and efficient peaks from gradients while seeing the quiet, stable baselines that maximize sensitivity. Better Resolution and Peak Shape Prevail Carbohydrate ES columns are versatile enough for mono- and oligosaccharides and sugar alcohols. They produce single peaks for reducing sugars at ambient temperatures, eliminating the need for column heating. Enhanced Detector Sensitivity Through Flat Gradient Baselines 9 98 8 9 Min. 8 9 Min. Column: Prevail Carbohydrate ES, μm,. x mm Mobile Phase: A: Acetonitrile B: Water Gradient: Time: %B: Flow Rate:.mL/min Detector: ELSD. Dextrose. Maltose. Maltotriose. Maltotetraose. Maltopentaose. Maltohexaose. Maltoheptaose 8. Maltooctaose 9. Maltononaose. Maltodecaose. Maltoundecaose. Maltododecaose. Maltotridecaose. Maltotetradecaose. Maltopentadecaose Robitussin Flu Syrup Min.. Sucralose. Fructose. Sorbitol. Glucose. Sucrose. Maltose Column: Prevail Carbohydrate ES, μm,. x mm Mobile Phase: A: Acetonitrile B: Water Gradient: Time: %B: Flow Rate:.mL/min Detector: ELSD Prevail Columns i.d. Length Particle Size (μm) C8 C8 Select C8 Phenyl CN Amino Silica Organic Carbohydrate ES Format Expedite. 8 8. 8 8. 88 88. 8 8 Microbore. 88 999 88. 8 99 8 888. 99 99 99 99 99. 99 99 998 99 999 99. 99 Solvent 99 99 99 998 99 Reducer 99 99 99 99 999 999 99 99 99 99 99 99 Analytical. 89 9 9 9 8. 99 99 99 99 99 999 88. 99 99 99 99 999 99 88. 9. 998 99 99 999 99 99 99 88. 99 99 999 99 99 99 99 88 Rocket 998 99 999 99 Guard*.. 98 99 998 99 99 99. 99 999 99 99 99.. 998 9 998 9988 9989 999 999 99 9 *Requires All-Guard Holder, 8. www.discoverysciences.com

& accessories GraceSmart Columns High Quality at Exceptional Value GraceSmart HPLC phases use high purity silica and are monomerically bonded with uniform coverage. This translates into symmetrical peaks for acids/bases and predictable reversed-phase selectivity. Whether for routine analysis or new method development, use GraceSmart columns to get premium performance at an exceptional value. GraceSmart Phase Specifications Pore Size (Å) Surface Area (m /g) Carbon Load (%) Endcapped Chromatographic Properties Phase C8 Yes High purity silica with monomeric C8 bonding offers classical reversed-phase selectivity. Application/ Benefit Excellent value for general purpose, routine applications. USP L-code L Consistent and Reproducible Our tightly controlled silica synthesis and bonding keep capacity factor and selectivity variations to a minimum for acidic, basic, and neutral compounds. Competitive Performance GraceSmart columns show similar or better efficiency and asymmetries for challenging base and acid components in comparison to industry leading columns. Capacity Factor..... 8 9 Toluene N-Butylbenzoic Acid Anisole Highly Reproducible Batch Acetophenone P-Nitrophenol Amitriptyline Symmetry Plates / Columns,, 8,,,,... Nicotine Nicotine Efficiency Quinine Symmetry Quinine Diphenhydramine Diphenhydramine Nortriptyline Nortriptyline Competitor C8 Column Competitor C8 Column, Dichlorophenoxy Acetic Acid, Dichlorophenoxy Acetic Acid, Dichlorophenoxy Propionic Acid, Dichlorophenoxy Propionic Acid, Dichlorophenoxy Butyric Acid, Dichlorophenoxy Butyric Acid Competitor C8 Column GraceSmart C8 Column Carbamates 8 8 Min. 9. Fenuron. Monuron. Fluometuron. Diuron. Methiocarb. Linuron. Neburon Folium Ginkgo..... Min.. Ginkgolide C. Bilobalide. Ginkgolide A. Ginkgolide B Column: GraceSmart C8, μm,. x mm Mobile Phase: A:.M KHPO, ph. B: Acetonitrile Gradient: Time: %B: Flow Rate:.mL/min Detector: UV at nm Column: GraceSmart C8, μm,.mm x Mobile Phase: Methanol:Tetrahydrofuran:Water (::) Flow Rate:.mL/min Detector: ELSD

& accessories mau - Column: Mobile Phase: Flow Rate: Detector: Amoxicillin (U.S.P.)........ Min. GraceSmart C8 Column Luna C8 Column Inertsil ODS- Column Hypersil BDS C8 Column Kromasil - C8 Column 9 GraceSmart C8, μm,. x mm Isocratic,.N Monobasic Potassium Phosphate ph :Acetonitrile (9:).mL/min UV at nm 8 Min. Standards Mix. Nicotine. Quinine. Diphenhydramine. Nortriptyline.,, Dichlorophenoxy Acetic Acid.,, Dichlorophenoxy Propionic Acid.,, Dichlorophenoxy Butyric Acid Column: GraceSmart C8, μm,. x mm Mobile Phase: % MeCN/% mm, KHPO, ph Flow Rate:.mL/min Detector: UV at nm Ibuprofen (U.S.P.) 9. Valerophenone. Ibuprofen Hypersil BDS C8 Kromasil - C8 USP GraceSmart Luna Inertsil Spec C8 C8 ODS- N/A.8..988..8 Ret. Time (min) Theoretical 8 9 8 plates Tailing...8...99 Factor k'. k'.8....99..... Min. Column: GraceSmart C8, μm,. x mm Mobile Phase: HO (adjusted with Phosphoric Acid to a ph of.): Acetonitrile (:) Flow Rate: ml/min Detector: UV at nm Water Soluble Vitamins 8 Min.. Ascorbic Acid. Nicotinic Acid. Pyridoxamine. Niacinamide. Folic Acid. Vitamin B. Riboflavin GraceSmart Columns Format i.d. Length Particle Size (μm) C8 Microbore.... 88. 88 Analytical. 8... 88. 88 Column: GraceSmart C8, μm,. x mm Mobile Phase: A:.M NHHPO Buffer, ph. B: Acetonitrile Gradient: Time: %B: Flow Rate:.mL/min Detector: UV at nm more info For detailed information on column formats, see page. technical assistance Contact Tech Support: Email: discoverysciences@grace.com Online: www.discoverysciences.com www.discoverysciences.com

& accessories Vydac MS Columns Unsurpassed Resolution, Sensitivity and Recovery for Biomolecules Vydac MS columns are the latest development in the ongoing effort to provide the best reversed-phase HPLC columns for biomolecules. A proprietary surface treatment and bonding process give Vydac MS columns truly unique selectivity. A variety of reversed-phases makes this product line suitable for small peptides to large intact, undenatured proteins. Vydac MS Phase Specifications Phase Pore Size (Å) Surface Area (m /g) Carbon Load (%) Endcapped Chromatographic Properties 8MS C8 8 Yes Polymeric bonding, highest hydrophobic interaction and unique geometric selectivity. 8MS C8 Yes Monomeric bonding offers increased peptide interaction and generally yields higher peak counts. 8MS C8 Yes Lower hydrophobicity is better for larger biomolecules. MS C Yes Lower capacity than C8 or C8, suitable for hydrophobic proteins or when minimal organic is desired. 9MS Diphenyl Application/ Benefit Use for simple enzymatic digests (< proteins) or biomolecules K MW. Use for same applications as 8MS, but offers different C8 selectivity. Ideal for biomolecules K MW. Ideal for biomolecules >K MW, undenatured intact proteins, antibodies, oligonucleotides, human growth hormone. Yes Lowest capacity, aromatic functional groups. Highly selective for proteins with aromatic side chains. USP L-code L L L L L Unique Selectivity Improved selectivity for peptides on the Vydac MS columns results in better primary structure definition and easier identification of degradation products and other protein characteristics. Tryptic Digest of Fetuin Vydac 8MS C8 Column Competitor C8 Column Competitor C8 Column 8Min. Columns: All Å, μm,. x mm Mobile Phase: A:.% v/v trifluoroacetic acid in HO B:.8% v/v trifluoroacetic acid in Acetonitrile Gradient: Time: 8 9 %B: 9 Flow Rate:.mL/min. Detector: UV at nm Sample: μl of a μg/μl digest (8μg total peptide load) Sample components interfere with the separation of peptides on the Competitor and columns, appearing as a chromatographic hump. The unique selectivity of Vydac MS columns solves separation problems. Unsurpassed Recovery and Peak Symmetry Many proteins are bound to the cell membrane and are very hydrophobic. Although hydrophobic proteins are particularly difficult to separate, Vydac MS columns provide excellent recovery and peak shape for these molecules. Transmembrane Protein p. 8.8 kd Protein. Non-myristolyated p. Myristolyated p. Triton X (Surfactant) Vydac MS C Column Competitor C Column Competitor C Column Competitor C Column Competitor C Column Min. Columns: All Å,. x mm packed with μm except μm for Competitor Mobile Phase: A:.% v/v trifluoroacetic acid in HO B:.8% v/v trifluoroacetic acid in Acetonitrile Gradient: Time: 8 %B: 8 8 9 9 Flow Rate:.mL/min. Detector: UV at nm The Vydac column provides better performance for this highly hydrophobic membrane protein (RRV p) and its fatty acid modified (myristolyated) form. 8

& accessories High Protein Recovery Unique surface chemistry reduces adsorption of proteins for higher recoveries and increases mass loading, which makes Vydac MS columns ideal for preparative chromatography. Excellent Peak Shape with Little or No TFA TFA is UV absorbent and contributes background at low UV wavelengths. It is additionally problematic with electrospray MS where it interferes with ion generation and reduces MS sensitivity. Percent Recovery 8 Vydac MS Column Loading Amount: μg of Cytochrome C Competitor Competitor Vydac MS C column provides more than % better recovery for Cytochrome C. Vydac MS Columns Competitive Recovery Comparisons i.d. Length Particle Size (μm) 8MS (C8) 8MS (C8) TFA Concentration Comparisons on 8MS Columns. AU...% TFA.% TFA.% TFA 8MS (C8) Min. MS (C) 9MS (diphenyl). Neurotensin 8 (fragment). Oxytocin. Angiotensin II. Neurotensin Format Capillary.. 9. 8. 8. 9. 8MS. 8MS. 8MS. MS.. 8MS. 8MS. 8MS. MS.. 8MS. 8MS. 8MS. MS.. 8MS. 8MS. 8MS. MS.. 8MS. 8MS. 8MS. MS.. 8MS. 8MS. 8MS. MS.. 8MS. 8MS. 8MS. MS.. 8MS. 8MS. 8MS. MS.. 8MS. 8MS. 8MS. MS.. 8MS. 8MS. 8MS. MS.. 8MS. 8MS. 8MS. MS.. 8MS. 8MS. 8MS. MS.. 8MS. 8MS. 8MS. MS.. 8MS. 8MS. 8MS. MS.. 8MS. 8MS. 8MS. MS.. 8MS. 8MS. 8MS. MS. Microbore. 8MS 8MS 8MS MS. 8MS 8MS 8MS MS 9MS. 8MS 8MS 8MS MS. 8MS 8MS 8MS MS Holder Solvent 8MS MS Reducer Analytical. 8MS MS. 8MS 8MS MS. 8MS 8MS 8MS MS 9MS. 8MS 8MS 8MS MS Preparative MS 8MS MS 8MS MS 8MS MS 8MS MS Guard.. 8GDMS 8GDMS 8GDMS GDMS 8.. 8GDMS 8GDMS 8GDMS GDMS 8 Prep Guard 8GCC GCC GCC 8GCC GCC www.discoverysciences.com 9

& accessories Vydac Everest Columns High Resolution for Complex Peptide Digests Everest columns have unique selectivity and sensitivity, which are the result of bonding technology that improves C8 surface coverage and deactivates residual silanols. Previously, the best Å C8 chemistries have had carbon coverage in the.8 to.μmol/m range. Everest C8 coverage is in excess of μmol/m and approximates the theoretical limit based on surface area. This increased shielding of the base silica increases column life and reduces the amount of TFA required to shield the silica. Vydac Everest Phase Specifications Pore Size (Å) Surface Area (m /g) Carbon Load (%) Endcapped Chromatographic Properties Phase 8EV C8 Yes Maximum surface coverage for highest resolution of complex samples. Application/ Benefit Complex enzymatic digests (> proteins). USP L-code L Tryptic Digest of Bovine Serum Albumin Everest Column peaks Commercial Column A 9 peaks Commercial Column B 9 peaks Min. Column: All C8, Å, μm,. x mm Mobile Phase: A:.% TFA in Water B:.8% TFA in Acetonitrile Gradient: Time: 8 9 %B: 9 9 Flow Rate:.mL/min Detector: UV at nm Everest columns outperform competitor columns by providing higher-resolution separations (average of % higher peak counts than competitor columns tested). Vydac Everest Columns i.d. Length Particle Size 8EV (C8) Format (μm) Capillary. 8EV.. 8EV.. 8EV.. 8EV.. 8EV.. 8EV.. 8EV.. 8EV.. 8EV.. 8EV.. 8EV. Microbore 8EV 8EV 8EV. 8EV. 8EV. 8EV. 8EV Analytical. 8EV. 8EV. 8EV Bovine Serum Albumin with no TFA Tryptic Digest Intensity (x cps) 8 9 Min. Column: Vydac Everest C8, Å, μm,. x mm Mobile Phase: A:.% v/v Formic Acid in Water B:.% v/v Formic Acid in Acetonitrile Gradient: Time: 8 9 %B: 9 9 Flow Rate: μl/min Detector: MS + ESI The Everest column performs exceptionally well with no TFA in the mobile phase, ensuring excellent microbore sensitivity. Vydac Everest Guard Columns i.d. Length Particle Size (μm) 8EV (C8) Holder Format Capillary. 8EVC GR-A Guard Guard.. 8GDEV 8.. 8GDEV 8 more info For detailed information on column formats, see page. technical assistance Contact Tech Support: Email: discoverysciences@grace.com Online: www.discoverysciences.com

& accessories Vydac ProZap C8 Columns Ultra Fast Protein and Peptide Separations The combination of ProZap.μm, Å packings and short Expedite hardware delivers not only faster sample throughput, but also low back pressures suitable for conventional LC systems. Under gradient conditions, longer columns only increase run time and do not increase resolution. Proteins adsorb at the head of the column and then desorb with the critical mobile phase concentration. Since proteins do not interact with the full length of the packed bed, short columns are sufficient for full resolution. Separate proteins of broad molecular weight ranges in less than one minute. Vydac ProZap C8 Phase Specifications Phase Pore Size (Å) Surface Area (m /g) Carbon Load (%) Endcapped Chromatographic Properties Application/ Benefit USP L-code C8 9 Yes Subμm, Å wide pore. Ideal for fast, intact protein or peptide analysis. L Seven Proteins in Under One Minute. Ribonuclease (ng). Bovine Insulin (ng). Cytochrome C (ng). Lysozyme (ng). Bovine Serum Albumin (ng). Carbonic Anhydrase (ng). Ovalbumin (ng) Synthetic Peptides Injections. Contaminant peptides/impurities. RGAGGLGLGK-amide (88 Da). Ac-RGAGGLGLGK-amide (9 Da). Ac-RGVGGLGLGK-amide (9 Da). Ac-RGVVGLGLGK-amide (99 Da).......... Min. Column: ProZap C8,.μm,. x mm, Å Mobile Phase: A:.% TFA in Water B:.8% TFA in Acetonitrile Gradient: Time:... %B: Flow Rate:.8mL/min Detector: UV at 8nm....8. Min. Antibodies in Sheep Serum. Albumin. IgG Column: ProZap C8,.μm,. x mm, Å Mobile Phase: A:.% TFA in Water B:.8% TFA in 8: Acetonitrile:Water Gradient: Time:..... %B: Flow Rate:.8mL/min Detector: UV at nm Vydac ProZap Columns i.d. Length Particle Size Format (μm) Expedite.. 8.. 8.. 8.. 88 Min. Column: ProZap C8,.μm,. x mm, Å Mobile Phase: A:.% TFA in Water B:.8% TFA in 9: n-propanol:water Gradient: Time:.... %B: Flow Rate:.mL/min Detector: UV at 8nm more info For detailed information on column formats, see page. www.discoverysciences.com

& accessories Vydac TP Columns The Industry Standard for Polypeptide Separations The Å pores of TP silica provides polypeptides complete access to the interior of the silica. The unique process by which we manufacture Vydac TP silica results in high purity with carefully controlled characteristics. It is the standard that has defined large pore HPLC silica for polypeptide separations for nearly two decades. Vydac TP Phase Specifications Pore Surface Carbon Phase Size (Å) Area (m /g) Load (%) Endcapped Chromatographic Properties Application/ Benefit 8TP C8 8 Yes First generation polymeric C8 Small polypeptides K MW, enzymatic digest fragments, L media with unique selectivity. natural and synthetic peptides, multi-ring compounds. 8TP C8 Yes First generation monomeric C8 Use for same applications at 8TP, but offers different L media. C8 selectivity. 8TP C8 Yes Less hydrophobic than C8TP Polypeptides K MW. L media. TP C Yes First generation C media. Glycoproteins, hemoglobin variants, histones, insulin L variants, membrane proteins. L 9TP Diphenyl Yes Lowest capacity first generation diphenyl media. Polypeptides with aromatic side chains, large hydrophobic proteins, membrane-spanning peptides, lipid peptides, fusion proteins from inclusion bodies. USP L-code Standard Human Apolipoproteins 8 9. apoc-iiia. apoc-iiib. apoc-iia. apoc-iiic. apoc-i. apoc-iib. apoa-ia 8. apoa-ib 9. apoa-iic. apoa-iia. apoa-iib The standard was human insulin. Insulin Variants 8. Chicken. Bovine. Ovine. Rabbit. Human. Porcine. Rat I 8. Rat II Min. Column: Vydac 8TP C8, μm,. x mm Mobile Phase: A: % ACN/.% TFA in Water B: 8% ACN/.% TFA in Water Gradient: Time: %B: Flow Rate:.mL/min Column Temp: C Min. Column: Mobile Phase: Vydac TP C, μm,. x mm A:.% TFA B: ACN with.% TFA Gradient: Time: %B: Comparison of Polypeptide Separations on C8, C, and Diphenyl Reversed-Phase Columns Tryptic Digest 8TP (C8). H. TH TP (C) 9TP (diphenyl) Min. Column: Vydac 8TP C8, μm,. x mm Mobile Phase: A:.% TFA B: ACN with.% TFA Gradient: Time: %B: Flow Rate:.mL/min Min. Columns: Vydac 8TP C8, TP C and 9TP diphenyl, all μm,. x mm Gradient: Time: %B: 9 Sample: 8-residue helical peptide () and six-helix template assembled synthetic protein ()

& accessories Monomeric and Polymeric Bonded Phases Comparison, 8TP Polymeric C8 8, 9 8TP Monomeric C8, 8 9 Min.. GY. VYV. Neurotensin fragment 8 (pelyenkpr). Ac-RGGGGLGLGK-NH. RGAGGLGLGK-NH. Ac-RGAGGLGLGK-NH. Ac-RGVGGLGLGK-NH 8. Oxytocin (CYIQNCPLG-NH) 9. Met Enkephalin (YGGFM). Bradykinin (RPPGFSPFR). Ac-RGVVGLGLGK-NH. Neurotensin Fragment 8 (RRPYIL). Angiotensin II (DRVYIHPF). Leu Enkephalin (YGGFL). Neurotensin (pelyenkprrpyil). Angiotensin I (DRVYIHPFHL) Columns: Vydac 8TP and 8TP, both C8, μm,. x mm Gradient: Time: %B: Flow Rate:.mL/min Detector: UV at nm. AU Purification of a Lipid Peptide.mg Preparative Chromatogram Min. Column: Vydac 9TP Diphenyl, μm,. x mm Mobile Phase: A: 9% n-propanol/.% HOAc/.% water B: % n-propanol/.% HOAc/8.% water Gradient: Time: %B: Flow Rate:.mL/min Detector: UV at 8nm A synthetic peptide containing amino acid residues and two attached fatty acids was difficult to purify because of its limited solubility and tendency to aggregate. The lower retention of the Vydac 9TP diphenyl reversed-phase column was useful for purification of this peptide after it was found to be retained so strongly that it could not be eluted from a C reversed-phase. Vydac TP Columns i.d. Length Particle Size (μm) 8TP (C8) 8TP (C8) 8TP (C8) TP (C) 9TP (Diphenyl) Format Nano 8TP 8TP 8TP TP 9TP 8TP 8TP 8TP TP 8TP 8TP 8TP TP 9TP 8TP 8TP 8TP TP 9TP Microbore. 8TP 8TP 8TP TP 9TP. 8TP 8TP TP 9TP. 8TP 8TP 8TP TP 9TP. 8TP 8TP 8TP TP 9TP Solvent Reducer 8TP 8TP TP 9TP 8TP 8TP 8TP TP 9TP Analytical. 8TP 8TP 8TP TP. 8TP 8TP 8TP TP. 8TP 8TP 8TP TP 9TP. 8TP 8TP 8TP TP 9TP. 8TP 8TP 8TP TP 9TP. 8TP 8TP 8TP TP 9TP Preparative 8TP TP 8TP 8TP 8TP TP 9TP 8TP TP 8TP TP 8TP TP 8TP TP 8TP 8TP TP 9TP 8TP Guard*.. 8GD 8GD 8GD GD 9GD.. 8GD 8GD 8GD GD 9GD 8 more info For detailed information on column formats, see page. technical assistance Contact Tech Support: Email: discoverysciences@grace.com Online: www.discoverysciences.com www.discoverysciences.com

& accessories Alltech IC Columns Separate Organic and Inorganic Anions and Cations Alltech Ion Chromatography Phase Specifications Phase Base Material Functional Group Anion Columns Allsep Anion Methacrylate Quaternary ammonium Novosep A- Polyvinyl alcohol Quaternary ammonium AN Polystyrenedivinylbenzene Dimethyl ethanol ammonium Anion/S Silica Quaternary ammonium Anion/R Polystyrenedivinylbenzene Trimethyl ammonium Cation Columns Universal Cation Universal Cation HR Specialty Columns Anion Exclusion Silica, μm Silica, μm Polystyrenedivinylbenzene Polybutadiene/ maleic acid copolymer Polybutadiene/ maleic acid copolymer ph Range Organic Modifier Limitations Application/Benefit % Meets requirements for EPA method. part A. Use for inorganic anions, weak and strong acid ions, metal complexes and organic acids % Ideal for separation of seven common anions plus three oxyhalide anions in one run. Meets multiple EPA method requirements. Use for inorganic anions, weak and strong acid ions, and organic acids. % Use for inorganic anions with the exception of fluoride. Silica-based for symmetrical peak shapes. % Larger dimensions ideal for high-resolution separations. Use for inorganic anions, weak and strong acid ions. % Separates Group I and II cations in one isocratic run. Separates transition metals without postcolumn reaction. EPA Method. part A Conductivity Mode suppressed and non-suppressed., suppressed.,.,. suppressed and non-suppressed non-suppressed non-suppressed. suppressed and non-suppressed % For high resolution applications.. suppressed and non-suppressed Sulfonate <% acetonitrile, no methanol, <% ethanol Use for organic acids and weak acid anions. Add small amounts of acetonitrile to decrease retention of hydrophobic molecules. Surfactant C8 Silica, μm C8 % Use for long chain or aromatic surfactants such as xylene sulfonate and dodecyl benzene sulfonate. non-suppressed non-suppressed Resolves Fluoride Away from the Water Dip Anions in High Ionic Strength Water by EPA Method.. Fluoride. Chloride. Nitrite. Bromide. Nitrate. Phosphate. Sulfate 8 9. Fluoride. Chlorite. Bromate. Chloride. Nitrite-N. Bromide. Chlorate 8. Dichloroacetate 9. Nitrate-N. Phosphate-P. Sulfate 8 8 Min. 8 8 Min. Column: Mobile Phase: Flow Rate: Detector: Allsep Anion, μm,. x mm.8mm NaHCO/.9mM NaCO.mL/min Suppressed Conductivity Column: Mobile Phase: Flow Rate: Detector: Novosep A- Anion, μm,. x mm.mm NaHCO.8mL/min Suppressed Conductivity