Question Answer Marks Guidance 1 (a) 1. 1 CREDIT herbivore / primary consumer,energy. trophic level 2 energy x 100 ; x 100 ; producer energy

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1 (a) 1 1 CREDIT herbivore / primary consumer,energy trophic level 2 energy x 100 ; x 100 ; producer energy trophic level 1 energy Plus any 3 of the following: CREDIT sample figures. e.g. if producer energy 20 000 kj m -2 and herbivore 2000 kj m -2 calculation is 2000 / 20000 x 100 = 10% CREDIT Energy available after transfer x100 Energy available before transfer IGNORE ref to productivity 2 (a sample of) producers collected ; 3 (a sample of) herbivores /primary consumers collected; CREDIT named examples for 2 and 3 ACCEPT 'organisms at each trophic level collected' for 1 mark 4 (collected from) the same area ; 5 (measure) biomass / dry mass (of individual or population) ; 5 ACCEPT wet / fresh,mass 5 IGNORE mass unqualified / pyramids of biomass 6 energy content calculated of producer and herbivore ; 6 ACCEPT expressed as J/KJ/MJ, per gram IGNORE calories per gram 7 use of calorimeter / described; 4 max 7 e.g. burn sample, in oxygen / in measure temperature increase ACCEPT use of published tables for energy values of, fresh /wet, mass

1 (b) Mark the first answer in each box. If the answer is correct and an additional answer is given that is incorrect or contradicts the correct answer then = 0 marks Goal improving soil that is low in nutrients for the growing of wheat preventing the spoilage of fruits after picking reducing the impact of a fungal disease on yields from cucumber plants producing strawberry plants that grow quicker and fruit earlier making sugar syrup from waste starch producing large amounts of a fungus for food Letter F ; E ; A / B ; A / B ; D ; C ; 6 ACCEPT A / B ACCEPT C

1 (c) Mark the first answer in each box. If the answer is correct and an additional answer is given that is incorrect Description Name or contradicts the correct answer then = 0 marks Sparrows initially fly away from fruit bushes on which shiny CDs are hung, particularly when the CDs move in the wind. After a few days the sparrows start visiting the fruit bushes again, and do not fly away even when the CDs move. Carrot flies move towards chemicals released by carrot plants. Raccoons learn to remove lids from containers of grain in a barn. A line of young chicks follow their mother into a cornfield. escape reflex ; habituation ; (positive chemo-) taxis operant conditioning / trial and error (learning) imprinting ; ; ; 5 Total 15 IGNORE innate / instinctive / learnt (as stated in Q) DO NOT CREDIT negative chemotaxis ACCEPT taxes CREDIT insight (learning) / latent (learning)/ intelligent learning / observational learning

2 (a) 8 Award 1 mark per row. biological principle letter artificial selection E ; predator-prey interaction G ; apical dominance B ; Mark the first answer in each box. If the answer is correct and an additional answer is given that is incorrect or contradicts the correct answer then = 0 marks nitrogen fixation and nitrification D ; reproductive cloning A / F ; positive chemotaxis H ; decomposition C / D ; commercial use of plant hormones F ; (b) 4 Mark the first answer on each prompt line. If the answer is correct and an additional answer is given that is incorrect or contradicts the correct answer then = 0 marks respiration / decomposition / decay / ripening ; ACCEPT metabolism / metabolic reactions interspecific competition ; (positive) phototropism ; DO NOT CREDIT negative phototropism DO NOT CREDIT trophism (as ambiguous with trophic) succession ;

(c) animals = primary consumers 3 1 keep animals, warm / indoors ; 2 reduce animal movement ; 3 feed animals high, protein / energy, food ; 4 vaccination / (routine) antibiotics, for animals ; 5 selective breeding / genetic engineering, for improved animals ; 2 ACCEPT zero grazing idea 3 ACCEPT growth-enhancing food additives 4 IGNORE hormones 5 ACCEPT description of improvement, e.g. disease resistant, faster-growing, higher yielding 6 slaughter just before, mature / full size ; Total 15

Question Answer Marks Guidance 3 (a) producer 3 (leaves / plants) fix carbon / photosynthesise / make food / autotroph(ic) / IGNORE first level in a food chain DO NOT CREDIT produces energy convert light energy to chemical energy / convert inorganic, C / CO 2, to organic molecules ; consumer (bird) eat / derives energy from / feeds on, other organisms or heterotroph(ic) ; trophic level stage / position / place / level, in a food, chain / web ; IGNORE consumes IGNORE named levels / organisms e.g. eats producers ACCEPT animals, and / or, plants IGNORE step, feeding level (b) ( 2 CREDIT any two correct answers number of quadrats (per area) ; method of placing quadrats (randomly) ; IGNORE ref to quadrats being the same size (as given in Q) time waiting, after solution added / for worms to rise ; volume of solution ; concentration of solution ; AVP ; IGNORE amount e.g. method of applying solution length of time spent counting time of day / light intensity soil moisture / rainfall / humidity method to ensure no double counting

Question Answer Marks Guidance 3 (b) (ii) means different / mean less in soil with plants removed ; 2 DO NOT CREDIT if difference in mean stated to be valid IGNORE average (but) error bars overlap ; (could have) mean trend reversed / equal numbers in some pairs of results ; difference, not / less, valid ; ACCEPT cross (over) e.g. in any pair of results you could find that the number of earthworms in the cleared soil could be higher than in the uncleared soil ACCEPT introductory statement No it is not. 3 (b) (iii) 2 Mark the first answer. If the answer is correct and an additional answer is given that is incorrect or contradicts the correct answer then = 0 marks number / abundance, of earthworms varies, from year to year / from 2004 to 2006 / over the two years / over time ; number / abundance, of earthworms varies, before and after plant clearance / as vegetation changes / during succession ; ACCEPT change described e.g. more worms in 2006 than 2004 If neither mark point awarded ACCEPT numbers of earthworms constantly, changing / fluctuating for 1 mark Total 9

Question Answer Mark 4 (a) ( succession ; Guid 1 FA IGNORE primary / secondary (ii) mineral content ; acidity / ph ; water depth; 2 FA (b) similarity chlorophyll breaks down / leaves change colour ; differences (bog) minerals stay in plant / (forest) minerals in soil ; ora decomposers / fungi / bacteria, not, present / active in bog ; ora for forest 1 2 FA for similarity Mark first two answers for differences ACCEPT named mineral ions in words or correct symbols ACCEPT decomposers / fungi / bacteria, break down leaves in forest (c) decomposers / named decomposers, not, present / active ; waterlogging reduces, air / oxygen ; 2 max ACCEPT (soil), bacteria / fungi / microbes can t survive or few can survive CREDIT waterlogging produces anaerobic conditions (d) acidity / low ph, stops (decay) enzymes working ; bog / habitat / ecosystem, takes a long time to form / hard to replace ; 2 loss of, biodiversity / rare species ; ACCEPT peat bogs maintain biodiversity Total 10