3M Petrifilm Salmonella Express System
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3M Petrifilm Salmonella Express System 3M Petrifilm Salmonella Express System is a qualitative test used for the rapid detection and biochemical confirmation of Salmonella in enriched foods and food process environmental samples, replacing traditional agar methods. o Sample ready media: 3M Petrifilm technology o Easy to use o No special equipment required o Allows for greater uniformity of results vs. dehydrated agar o Longer shelf life compared to agar plates o Compact/stackable plate & disk reduce waste 3 o All-in-one test method: provides presumptive and biochemical confirmed results o Quicker time to biochemical confirmed result (44-52 hours) o Confirms all circled presumptive positive colonies on plate all at the same time
3M Petrifilm Salmonella Express System Method Overview Two Methods: High microbial background vs. Low microbial background o Both methods use 3M Salmonella Enrichment Base + 3M Salmonella Enrichment Supplement o Only high microbial background method uses R-V R10 as secondary selective enrichment o Single streak method for plating o Single incubation temperature of 41.5 ± 1 C throughout method o Flexible range of incubation time to support laboratory flexibility 4
3M Petrifilm Salmonella Express System Matrices Evaluated in Method Validation High Microbial Load >10 4 CFUs Raw Meats Ground chicken Ground beef Ground pork Seafood Frozen, uncooked shrimp Fresh Produce Spinach Low Microbial Load <10 4 CFUs Processed Meats Cooked chicken nuggets Egg Products Pasteurized, whole liquid eggs Pet Food Dry dog food Environmental Stainless Steel 5
6 3M Food Safety 3M Petrifilm Salmonella Express System Procedure Media Preparation & Enrichment Procedure
3M Petrifilm Salmonella Express Plate Hydration Procedure o Hydration Diluent = reverse osmosis water, distilled water or Butterfield s Phosphate Diluent 7
3M Petrifilm Salmonella Express Plate Qualifying Plate: Hydrated Plate Color Card o o o A Hydrated Plate Color Card will be placed in each Box and Case of the 3M Petrifilm Salmonella Express Plates. This is a great tool for incoming inspection to quickly check if the plate is acceptable for use. If Hydrated Gel is Red or Bright Pink, plates are acceptable for use. If the Hydrated Gel is orange or brown, plates should NOT be used for testing due to improper storage or transit (extended exposure to high temperatures). Hydrated Gel Area 8
9 3M Food Safety 3M Petrifilm Salmonella Express System Plate Inoculation, Incubation and Interpretation
Identifying Salmonella Species The following are presumptive positive Salmonella species: The following are Non- Salmonella species Blue colonies, green colonies, blue to green colonies, and/or black colonies with or with out a yellow zone and/or associated gas bubble Red, dark red and brown colonies with out a yellow zone and/or associated gas bubble Red, dark red and brown colonies with a magenta zone 10 Must circle presumptive positive Salmonella colonies prior to disking
3M Petrifilm Salmonella Express System Procedure Biochemical Confirmation 11
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3M Petrifilm Salmonella Express System Procedure Biochemical Confirmation The biochemical confirmation result of the 3M Petrifilm Salmonella Express System is statistically equivalent to the biochemical results of the reference methods. What does it compare to? Standard method testing through biochemical identification (Vitek or API). Not equivalent to O&H serological results. 13
3M Petrifilm Salmonella Express System Procedure A Comparative Evaluation of the 3M Petrifilm Salmonella Express System for the Detection of Salmonella species in Food and Environmental Surfaces AOAC Method Validation Testing conducted by third party reference lab Comparison testing to reference methods: USDA/FSIS-MLG 4.05 (2011) Isolation and Identification of Salmonella from Meat, Poultry, Pasteurized Egg and Catfish Products FDA/BAM Chapter 5 Salmonella method ISO 6579: Microbiology of food and animal feeding stuffs-horizontal method for the detection of Salmonella spp. 14
3M Petrifilm Salmonella Express System Organisms for Inoculation Matrix Inoculating Organism Matrix Inoculating Organism Raw Ground Chicken Pasteurized Liquid Whole Eggs Dry Dog Food Raw Ground Pork Stainless Steel Environmental Surface Salmonella enterica spp. Heidelberg NCTC 5717 Frozen, Uncooked Shrimp Salmonella enterica spp. Virchow ATCC 51955 Salmonella enterica Salmonella enterica spp. Enteritidis Fresh Spinach spp. Saintpaul ATCC 13076 ATCC 9712 Salmonella enterica spp. Poona NCTC 4840 Salmonella enterica spp. Montevideo ATCC 8387 Salmonella enterica spp. Kahla ATCC 17980 Raw Ground Beef Cooked Chicken Nuggets Salmonella enterica spp. Ohio STS 81* Salmonella enterica spp. Typhimurium ATCC 14028 15
3M Petrifilm Salmonella Express System Fractional Food Matrices & Comparative Results Food matrix 3M Petrifilm Salmonella Express System Presumptive/Confirmed Reference Method (BAM or USDA) Presumptive /Confirmed ISO 6579 Reference method Raw ground chicken 15/15 14/14 14/14 Liquid whole egg 6/6 4/4 5/5 Fresh frozen shrimp 12/12 10/10 Spinach 11/11 10/10 Dry dog food 16/16 14/14 Raw ground beef 13/13 13/13 Frozen chicken nuggets 6/6 6/6 Raw ground pork 9/9 9/9 Stainless steel 7/7 6/6 16
3M Petrifilm Salmonella Express System Procedure Summary of Results - 3M Petrifilm Salmonella Express System Performance No statistically significant difference in the number of positive samples detected by the 3M Petrifilm Salmonella Express System and the reference methods for all matrices analyzed. 17 Summary from 3 rd Party Reference Lab The 3M Petrifilm Salmonella Express System demonstrated reliability as an alternative qualitative identification method for the detection and biochemical confirmation of Salmonella in select foods and environmental surface.
3M Petrifilm Salmonella Express System Validation: 3 rd Party Reference Lab Summary o o o o o For all matrices tested, the 3M Petrifilm Salmonella Express method was equivalent to the reference methods There were no false positive results Easy to Streak Like Single Streak Method Easy to interpret High background foods were easier to interpret on the 3M Petrifilm Salmonella Plate compared to the Reference Method Faster time to result Like the [3M Petrifilm Salmonella Express] Confirmation Disk compared to the Reference method This is a great product and the 3M team should be very proud --Pat Bird, Study Coordinator, Q lab, USA 18
3M Petrifilm Salmonella Express System Method Approvals 3M is pursuing method approvals recognized globally AOAC Research Institute Performance Tested Method SM #061301; Validated June 3, 2013 o AOAC Research Institute OMA in progress o ISO 16140 in progress The 3M TM Petrifilm TM Plates are the #1 brand in food indicator testing. Ninety-one out of 100 of the Top Food Processing Companies in the U.S. use 3M Petrifilm Plates for their testing.* 19 *According to Food Magazine Top Food Processors
(Low background, processed foods Method) TOTAL TIME TO NEG: 40-44 HRS (High background, unprocessed foods Method) TOTAL TIME TO NEG: 48-52 HRS 20 Biochemical Confirmation
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3M Petrifilm Salmonella Express System Shelf Life & Storage Temperature 24
3M Petrifilm Salmonella Express System Estimated Number of Tests Per Packout Catalog Number 70# Product Name Packout TOTAL TESTS (Based on 225 ml) 6536 70-2007-6998-5 3M Petrifilm Salmonella Express Plate 25 plates/ pouch, 2 pouches /box 50 6537 70-2007-6999-3 3M Petrifilm Salmonella Express Plate 25 plates/ pouch, 8 pouches /case 200 6538 70-2007-7000-9 3M Petrifilm Salmonella Express Confirmation Disk 5 disks/ pouch, 1 pouches /box 5 6539 70-2007-7001-7 3M Petrifilm Salmonella Express Confirmation Disk 5 disks/ pouch, 5 pouches /case 25 SEB500 70-2007-7477-9 3M Salmonella Enrichment Base 500g/bottle 1 ea 60 SEB025 70-2007-7478-7 3M Salmonella Enrichment Base 2.5kg/bottle 1ea 300 SESUP001 70-2007-7435-7 3M Salmonella Enrichment Supplement 1g/vial 1ea 88 BPO288500 70-2007-5512-5 3M R-V R10 (Secondary Enrichment for High Microbial Load samples only) 500g/bottle 1880 25
3M TM Petrifilm TM Salmonella Express System 26