Organelle Structure and function Organelles Molecules Cellular function Ch 5: Cells, the working units of life Ch 27: The origin and diversification of Eukaryotes
Discussion Summary: Week 2 Cell Biology s Open Cases Team Discussion: Related topics Questions Take home message Group discussion Type the take-home messages in D2L or write on board Write questions on the board Brief review of relevant facts deemed important by instructor.
Organelle Structure and Function Re-cast this idea as: Cellular Function(s) Molecular basis Organelles role(s) In terms of organismal/evolutionary study Structures and traits
h"p://bio1151.nicerweb.com/locked/media/ ch27/capsule.html h"p://www.sigmaaldrich.com/technical- documents/ar7cles/biology/glycobiology/ pep7doglycans.html Prokaryotic cell features Review of form, molecules and function
h"p://www.bmb.leeds.ac.uk/illingworth/ 6form/ Prokaryotic cell features Review of form, molecules and function
Eukaryotic Cells: Classic and EM views FUNCTION relates to STRUCTURE
Studying Organelles: Physical and chemical properties Isolate associated molecules
Highlights: Endomembrane system Membraneassociated protein production Organelles, molecules, FUNCTIONS Lipid synthesis Protein modification
Special Plant Organelles Unique chemistry Important functions Plastids Glyoxysome Which one not on this slide?
Unsung heroes! The other organelles and structures Peroxisome Many special reactions here Catalase breaking down H2O2 quite important! h"p://flipper.diff.org/app/items/3693
Unsung heroes! The other organelles and structures Extracellular Structures How do molecule get outside the cell? How are they assembled into structures?
Unsung heroes! The other organelles and structures Cytoskeleton So many types! What does this mean about function?
Unsung heroes! The other organelles and structures Cytoskeleton How can we study function? Hypothesisdriven expeiment
Protists Insight into evolution Organelles O2, ATP, Membranes Multicellularity Single-celled organisms useful for investigation Diversity sparks question about function Ecological impact
Protists Insight into evolution Organelles O2, ATP, Membranes Multicellularity
Protists Single-celled organisms useful for investigation Use of yeast Use of chemistry Visualization of molecules and chemical properties elegant experiment!
Protists: MCB and OEB Diversity sparks questions about function Classical TRAITS are cellular Classification Evolution Phylogenies MOLECULAR mechanisms underlie structure and function Biochemistry DNA SEXUAL REPRODUCTION leads to diversity
Protists interaction with environment Ecological impact Geological Biochemical Symbiosis Disease Mutualism Etc. h"p://www.treadright.org/project/ white- cliffs- dover h"p://earthobservatory.nasa.gov/ Features/Phytoplankton/page2.php
Protists interaction with environment Ecological impact Geological Biochemical Symbiosis Disease Mutualism Etc. h"p://www.treadright.org/project/ white- cliffs- dover h"p://earthobservatory.nasa.gov/ Features/Phytoplankton/page2.php
STRETCH BREAK!
Investigating Life : Chapter 1 Figure 1.12 Gather related and necessary facts to set up and understand experiment (Fig 1.11) Discuss data prediction (deductive reasoning) Discuss actual data and conclusions
Investigating Life : Chapter 1 Figure 1.11 needed as reference for 1.12 Observations during first experiment help form hypothesis about next experiments Come up with ideas about how things work (inductive reasoning conclusions from observations or experiment) Note experiment types: Controlled or comparative
This week s lab: Lab 1b, 1c intro How was pre-lab? Planning your preparations and views Some questions Some predictions Use last week s data! Using the digital camera Logging your images Fluorescence and Phase-Contrast microscopy Questions??
PROCEDURE: Obtain lab(s) from course website Complete PRE-LAB - Summary section - Detailed method, data section - Questions Create digital version of PRE-LAB - Upload to D2L, group area by 8am Tuesday Review and assess partner s: - Tuesday afternoon meeting recommended Upload RUBRIC to D2L - Edited PRE-LAB secured in lab notebook by 8am Thursday. - Incorporate coments from partner. REVIEW of PRE-LAB requirements
Next week s lab: Lab 2 : Protein quantitation. Introduction 4 major molecules in biology Spectrophotometry, standard curve, dilutions, use of calculations Proteins have numerous functions in cells, and measuring protein is part of molecular biology and/or biochemistry How much protein per serving in a supplement? h"p://www.di.uq.edu.au/sparqspectro
Next week s lab: Lab 2 intro Future labs will use these techniques Dilutions create varying concentrations of standard (BSA), and measurable amounts of unknown. Bradford assay is Coomassie Blue & Spectrophotometry h"p://www.qcbio.com/pierce/23236.htm
Next week s lab: Lab 2 intro Future labs will use these techniques Bradford assay is Coomassie Blue & Spectrophotometry h"p://www.di.uq.edu.au/sparqspectro