Sientifi Ppers of the R.I.F.G. Pitesti, Vol. XXV, 9 COMPORTAREA SPECIEI PRUNUS SERRULATA VAR. KANZAN ÎN FAZELE DE ÎNRĂDĂCINARE IN VITRO ŞI ACLIMATIZARE PRUNUS SERRULATA VAR. KANZAN BEHAVIOUR REGARDING IN VITRO ROOTING AND ACCLIMATIZATION Mgdlen Duţă, Mihel Ilen Opre, Mnuel Elen Conioiu University of Pitesti - Romni Astrt: Studies regrding the ehvior of Prunus serrult in vitro ulture hve een undertken in the Vegetl Biotehnologies Lortory, t the University of Pitesti, sine 7. The pper presents spets regrding in vitro rooting nd limtiztion perentges of Prunus serrult vr. Knzn vitroplnts. During in vitro rooting stge, good results were otined using nutrient medium with the following omposition: hlved Murshige - Skoog minerl slts, Linsmier Skoog vitmins, gierelli id (GA).1 mg/l, indoleleti id (IAA) 1. mg/l, tivted ron.3 mg/l, iron helte 38 mg/l, g/l dextrose, 7 g/l gr. The sustrte reommended for limtiztion of in vitro plnts is omposed of lk pet + perlite (1:1), t ph = 6.. Cuvinte heie: Prunus serrult vr. Knzn, înrădăinre, uxine, limtizre, Keywoords: Prunus serrult vr. Knzn, rooting, uxins, limtiztion 1. Introdution Prunus serrult Knzn is one of the most elegnt nd ppreited ornmentl herries, the deortive element of the plnt eing represented y long lef stlk, deep pink flowers. They re doule, ig, long lef stlk, undnt, with deep pink olor, swinging. Their lossoming is lso very profuse. The use of onventionl methods, to multiply this dendrologil vriety hs very wek onveyne effiieny. At the University of Pitesti, within the lortory of Vegetl Biotehnologies we undertook studies for the purpose of estlishing the in vitro reprodution iotehnology of the Prunus serrult speies. 2. Mteril nd methods: For in vitro rooting of ornmentl herry mirouttings resulted from the in vitro multiplition phse were tested six experimentl vrints (Tle 1). The experiene is iftoril. A Ftor : The rooting nutrient medium ] A.1 ] A.2 ] A.3 B Ftor : Photoperiodism ] B.1 14 hours photoperiodism ] B.2 12 hours photoperiodism For ll nutrient medium vrints, the si omponents, mroelements, miroelements, vitmins, gierelli id, iron helte, dextrose, gr nd tivted ron re onstnt nd hve the sme onentrtion, the vrile ftor is represented y uxin s onentrtion (AIA) (Tle 2). For vitroplnts limtiztion, the experiene ws monoftoril, eing tested two nutrient sustrtes (Tle 3). During limtiztion, vitroplnts were pssed on sustrte with light texture, treted with rooting iostimultors, ensuring high tmospheri humidity (8%) nd onstnt temperture. Results were reorded s rooting perentge, respetively limtiztion nd were proessed sttistilly y Dunn test. 3. Results nd disussions Anlyzing the intertion etween the nutrient medium nd photoperiodism (A x B), the results otined hve shown tht the nutrient medium hs signifint influene, the most effetive one proved to e A.2 with 82% rooted plnts, followed y nutrient medium A.3 with 74% rooted plnts (Fig. 1). Interpreting the dt regrding the intertion of eh nutrient medium with eh photoperiodism grdution it ws found tht for photoperiodism of 14 hours, the est results with 85.% rooted plnts otined on nutrient medium A.2. Differenes from other nutrient medi re signifint nd provided sttistilly. 2
Sientifi Ppers of the R.I.F.G. Pitesti, Vol. XXV, 9 For the 14 hours photoperiodism, the influene of nutrient medi A.1, A.2 nd A.3 on the in vitro rooting proess is greter thn photoperiodism of 12 hours. In this se we n stte tht the nutrient medi tested re more effetive t 14 hours photoperiodism (Fig. 2). The rooting period lsted dys. Plnts otined t the end of this in vitro ulture stge replite ll the vegettive orgns of vitroplnts (Fig. 3). Looking t the intertion etween the two nutrient mixtures tested s support for limtiztion of "Knzn" vriety vitroplnts, s n verge effet, it is found tht the highest degree of limtiztion ws determined y A.1 nutrient mixture. The influene of this nutritionl support is quntified to 72.% of plnts dpted, the differene from the other presription eing provided sttistilly (Fig. 4). The explntion of otining the highest perentge of limtized plnts on nutrient medium A.1 is given y the presene in the nutrient omposition of lk pet nd perlite, proportion of 1:1, providing ontinuous moisture to the sustrte, omprle to tht of ulture vessels where plnt roots ourred. Alimtiztion phse ws mrked y the onset of plntlets tive growth, emerging the first leflets. Alimtiztion period lsted 15 dys. 4. Conlusions: Studies regrding the ehvior of Prunus serrult in vitro ulture led to the following onlusions: 1. For the rooting phse results onfirm tht the uxins hve sustntil role in triggering nd sustining the rhizogenesis proess, within ertin onentrtions. 2. Interpreting the intertion etween the nutrient medium nd photoperiodism in rooting proess, the vrint with the highest perentge of rooting is V.3 respetively 85%, on nutrient medium with 1 mg/l IBA nd photoperiod of 14 hours. 3. The gretest limtiztion degree hs ourred on nutrient mixture A.1, ontining lk pet nd perlite 1:1. Referenes: 1. Is, M., 1983. Comportre unor soiuri de prun in fz de multiplire in vitro, Pitesti Lurările elui de l II le Simpozion Nţionl De Culturi De Ţesuturi Vegetle In Vitro, Vol. II, 212-218 2. Linsmier, E. M., Skoog, F., 1965. Orgni growth ftor requirements of too tissue ultures. Physiol. Plnt, -127. 3. Ones, O.P., Hopgood, M.E, 1979. The suessful propgtion in vitro of two rootstoks of Prunus: the plum rootstok Pixy (P.insititi) nd the herry rootstok F12/1 (P.vium). Journl of Hortiulturl Siene 54 (1), 63-66. 4. Pinker., L, 1997. Mikrovermehrung von Prunus serrult Knzn, Deutshe Bumshule, 8: 426-427. 5. Posedru, E.A., 5. Plnte ornmentle reomndte pentru menjre spţiilor verzi din Români. Ed. Universităţii din Piteşti, 137-1. 6. Posedru E.A., Militru, M., 1. Studii u privire l sigurre deorului u speii şi vrietăţi dendrologie vlorose în prul dendrologi Piteşti-Mărăineni. Anlele Universităţii din Criov, Vol. VI (XLII), I.S.S.N. 1435-1275. Tles nd Figures Tle 1. Experimentl vrints for in vitro rooting phse Vrints Vrile ftors A: Nutrient medium B: Photoperiod V.1 A.1 B.1 V.2 A.1 B.2 V.3 A.2 B.1 V.4 A.2 B.2 V.5 A.3 B.1 V.6 A.3 B.2 3
Sientifi Ppers of the R.I.F.G. Pitesti, Vol. XXV, 9 Tle 2. Nutrient medi omponents tested for ornmentl herry in vitro rooting Components mg/l Medium vrints A1 A2 A3 M.S Mroelements 1/2 1/2 1/2 M.S Miroelements 1/2 1/2 1/2 L.S Vitmins n n n Gierelli id.1.1.1 AIA.5 1. 1.5 Ativted ron.3.3.3 NFeEDTA 38 38 38 Dextrose Agr 7 7 7 Legend: M.S Murshige & Skoog, L.S - Linsmier & Skoog Tle 3. Nutrient sustrtes tested for ornmentl herry vitroplnts limtiztion Vrile A ftor Sustrte omponents Components proportion ph A.1. Blk pet (Bioln) + Perlite 1:1 6. A.2. Red pet (Bioln) + Perlite 1:1 5. 9 8 Dunn test (P<,5) B1 B2 Medium effet A1 57. 52. 54.5 A2 85. 79. 82. A3 76. 72. 74. Fig. 1. The vrition of in vitro rooting perentge depending on the nutrient medi, for different photoperiods 4
Sientifi Ppers of the R.I.F.G. Pitesti, Vol. XXV, 9 9 8 Dunn test (P<,5) A1 A2 A3 Medium effet B1 - Photoperiod -14 h 57. 85. 76. 72.7 B2 - Photoperiod -12 h 52. 79. 72. 67.7 Fig. 2. The vrition of in vitro rooting perentge depending on the photoperiod for different nutrient medi Fig. 3. In vitro rooted plnt 5
Sientifi Ppers of the R.I.F.G. Pitesti, Vol. XXV, 9 8 Dunn test (P<,5) Perent of limtiztion V1 V2 72.. Fig. 4. The vrition of dpted plnts perentge depending on the nutrient medium Fig. 5. Alimtized plnts 6