Put the C18 aside and take a look... 6 Reasons to try the EW Gemini
Reason #6 ALTERATE SELECTIVITY to your C18 Column Adding to your method development options, Gemini offers additional polar and aromatic selectivity, which may not be possible with a traditional C18 column. mau 60 40 20 0 Gemini 5µm 1 0 2 4 6 8 10 12 14 16 mau 60 40 20 1 2a 2b 3* 4 Gemini 5µm C18 3 2b 2a 4 APP ID 15889 APP ID 15887 min 0 0 2 4 6 8 10 12 14 16 min * otice the improved resolution of closely eluting beta blockers with Gemini. Mobile Phase: 10mM Ammonium Bicarbonate, ph 10.5/Methanol (50:50) Flow Rate: 1.0mL/min Detection: UV @ 230nm Sample: 1. Atenolol 2. Labetolol (diastereomers) 3. Pindolol 4. Metoprolol
Reason #5 HYDROPHOBIC RETETIO similar to a C8 Reason #4 LOW BLEED Phenyl Phase To achieve dual selectivity (of both a phenyl phase and a short alkyl phase) Gemini uses a 6-carbon linker to attach the phenyl group to the silica surface. Compared to most other phenyl phases, which use a short 2 or 3 carbon linker, you will find this stationary phase a versatile tool for both your phenyl AD C8 methods. One of the weaknesses of traditional phenyl phases has been excessive bleed in UV and MS. Gemini demonstrates far lower bleed than many other phenyl columns. Indeed, in this example shows lower bleed than a popular C18 column. Tricyclic Antidepressants RESOLUTIO XTerra 5µm Phenyl Gemini 5µm Rs 1,2 1.8 4.1 Rs 2,3 1.6 2.8 Rs 3,4 3.9 7.1 Mobile Phase: 10mM Ammonium Bicarbonate, ph 10.5/Acetonitrile/Methanol (30:35:35) Flow Rate: 1.0mL/min Detection: UV @ 254nm XTerra 5µm Phenyl () Gemini 5µm () Sample: 1. Imipramine 7200 9200 2. ortriptyline 8700 12000 3. Amitriptyline 8500 11700 4. Clomipramine 8800 11700 Average: 8300 11150 APP ID 15885 APP ID 15886 Dimensions: 150 x 3.0mm Mobile Phase: A: 0.1% Formic acid in Water B: 0.1% Formic acid in Acetonitrile Gradient: 5%B to 95%B in 10 min, then hold 95% B for 2min Flow Rate: 0.6mL/min MS Detection: ESI + ion mode, M/Z 100-700 Phenomenex Web: www.phenomenex.com
Reason #3 EHACED PERFORMACE for Aromatic Compounds Gemini is based on our revolutionary patent-pending Two-In-One (TWI ) particle technology that not only gives wide ph stability, but also delivers excellent performance that is not matched by other phenyl phases. Sulfa Drug Application RESOLUTIO Pursuit 5µm DiPhenyl Gemini 5µm Rs 1,2 1.0 4.0 Rs 2,3 9.8 16.0 2Reason #2 REPRODUCIBLE Phenyl Phase Gemini incorporates the same tightly controlled silica manufacturing procedures as our world renowned Gemini C18 phase to deliver reproducible performance you can count on: column-to-column, batch-to-batch. Aliphatic Acid Application APP ID 15903 APP ID 15908 Sulfathiazol O O H 2 S Sulfamerazin H O S S O H Mobile Phase: 0.1% Formic Acid/Methanol (70:30) Flow Rate: 1.0mL/min Detection: UV @ 254nm Sample: 1. Sulfathiazole 2. Sulfamerazine 3. Sulfamethoxazole CH 3 Mobile Phase: 20mM Phosphate buffer, ph2.5/methanol (97:3) Flow Rate: 1.0mL/min Detection: UV @ 220nm Sample: 1. Tartaric Acid 2. Malic Acid 3. Lactic Acid 4. Acetic Acid 5. Citric Acid 6. Propionic Acid H 2 Phenomenex Web: www.phenomenex.com
Reason # 1 ph STABLE from 1-12 200000 Accelerated ph Stability Test, ph 10.5 180000 160000 140000 120000 Efficiency () 100000 80000 60000 40000 20000 Gemini 5µm Gemini 5µm C18 0 0 500 1000 1500 2000 2500 3000 3500 4000 min Mobile Phase: A: 10mM H 4 HCO 3, ph 10.5 B: 10mM H 4 HCO 3, ph 10.5/Acetonitrile (90:10) Gradient: 0%B to 100%B in 10min, hold at 100%B for 7min, hold at 0%B for 3min Flow Rate: 1mL/min Temperature: 50 C Detection: UV @ 254nm Sample: Prednisolone
CHARACTERISTICS Gemini Low High Hydrophobicity Gemini Polarity H-Bonding Aromatic Selectivity USP: L11 Silanol Activity (ph 2.5) LC/MS Certified ph Stability 1.0 12.0 Particle Size 3µm and 5µm Phase Application Strength Phenyl with C6 linker Aromatic, polar or basic compounds High aromatic selectivity with exceptional peak shape even in neutral conditions. Extremely low bleed phenyl column. R Phenyl Si R O C6 Linker
ORDER IFORMATIO Gemini 3µm MercuryMS (mm) MercuryMS cartridge requires holder, Order o.: CH0-5845 20 x 2.0 20 x 4.0 20 x 2.0 20 x 4.0 Columns Cartridges 00M-4443-B0 00M-4443-D0 00M-4443-B0-CE 00M-4443-D0-CE 3µm Minibore Columns (mm) SecurityGuard Cartridges 30 x 2.0 50 x 2.0 100 x 2.0 150 x 2.0 4 x 2.0 Gemini 00A-4443-B0 00B-4443-B0 00D-4443-B0 00F-4443-B0 AJ0-7914 for ID: 2.0-3.0mm 3µm Analytical Columns (mm) SecurityGuard Cartridges 50 x 3.0 100 x 3.0 150 x 3.0 4 x 2.0 Gemini 00B-4443-Y0 00D-4443-Y0 00F-4443-Y0 AJ0-7914 for ID: 2.0-3.0mm SecurityGuard Cartridges 30 x 4.6 50 x 4.6 100 x 4.6 150 x 4.6 4 x 3.0 00A-4443-E0 00B-4443-E0 00D-4443-E0 00F-4443-E0 AJ0-7915 for ID: 3.2-8.0mm 5µm Minibore Columns (mm) SecurityGuard Cartridges 50 x 2.0 100 x 2.0 150 x 2.0 250 x 2.0 4 x 2.0 Gemini 00B-4444-B0 00D-4444-B0 00F-4444-B0 00G-4444-B0 AJ0-7914 for ID: 2.0-3.0mm 5µm Analytical Columns (mm) SecurityGuard Cartridges 50 x 3.0 150 x 3.0 250 x 3.0 50 x 4.6 150 x 4.6 250 x 4.6 4 x 3.0 Gemini 00B-4444-Y0 00F-4444-Y0 00G-4444-Y0 00B-4444-E0 00F-4444-E0 00G-4444-E0 AJ0-7915 for ID: 3.2-8.0mm XTerra is a registered trademark of Waters Corp. Pursuit is a trademark of Varian. Gemini, Twin, Two-in-One Technology are trademarks of Phenomenex. Phenomenex is not associated in any way with Waters or Varian. Data on previous pages may not be representative of all applications. 2005 Phenomenex Inc. All rights reserved. SecurityGuard cartridge requires holder, Order o.: KJ0-4282 If you are not completely satisfied with Gemini after using for 45 days, KEEP THE COLUM FOR FREE Expires FREE Soccer Ball Offers apply to Gemini columns <10mm ID. 15 December 2005 Phenomenex Web: www.phenomenex.com
3953_I_ FREE Soccer Ball Offers apply to Gemini columns <10mm ID.