Multiple stressor effets of ionising (gmm) rdition nd non-ionising (UV) rdition in dukweed (Lemn minor) Li Xie 1,2,3, You Song 1,3, Knut Asjørn Solhug 2,3, Ole Christin Lind 2,3, Brit Slu 2,3, Bjørn Johnsen 4, Knut Erik Tollefsen 1,2,3 1 Norwegin Institute for Wter Reserh (NIVA) 2 Fulty of Environmentl Sienes nd Nturl Resoure Mngement (MINA), Norwy Norwegin University of Life Sienes (NMBU) 3 Centre for Environmentl Rdiotivity (CERAD), Norwegin University of Life Sienes (NMBU) 4 Norwegin Rdition protetion Authorty (NRPA) Contt : Li.Xie@niv.no
Ionizing nd Non-Ionizing rdition Ionizing Rdition X Rys Gmm Rys Visile Infrred Ultrviolet Ner Fr Nonionizing Rdition Rdr FM TV Short wve Cosmi Rys Brodst 10-14 10-12 10-10 10-8 10-6 10-4 10-2 1 10 2 10 4 10 6 Wvelength in Meters
The hllenge Wht will hppen in the quti eosystem? Additivity (1+1=2) Antgonism (1+1<2) Synergy (1+1>2)
Dukweeds (Lemn minor) Centrl funtion in quti eosystems Esily ultured in ontrolled lortory onditions Stndrdised protools ville for regultory testing Rpid reprodution pity Highly sensitive to ontminnts Genome prtly sequened, moleulr tools prtly ville
Ojetive Study Clrify the individul nd omine effet of -rdition nd UVR in the dukweed Modeling Develop dverse outome pthwy (AOP) frmework Applition Hzrd Assessment Stressor Moleulr Inititing Event Key Events Adverse Outome Popultion effet
Approh Study 1: UV rdition effets Study 2: Gmm rdition effets 60 Co 60 Co UV Chmer Study 3: UV nd gmm rdition omined effets Endpoints: Growth ROS formtion Lipid peroxidtion OXPHOS PSII effiieny Pigments DNA dmge Gene expression Gmm dose rte D Wter (mgy/h) Gmm totl dose D Wter (mgy) 13.2 2195 20.3 3375 47.1 7831 UV Dose rte (W/m 2 ) 0.5±0.03 Sttistis Two-wy ANOVA
R e p ro d u tio n In h i itio n (% ) F r o n d s iz e in h i ito n (% ) F re s h w e ig h t in h i itio n (% ) Growth Reprodution Frond size Frond weight 4 0 3 0 2 0 1 0 0-1 0 2 0 1 5 1 0 5 0-5 3 0 2 0 1 0 0-1 0 -r d i tio n r te (m G y /h ) -r d i tio n r te (m G y /h ) -r d i tio n r te (m G y /h ) UV did not interrupt gmm rdition effets on frond size nd weight ut reprodution rte.
R O S (F o ld In re s e ) L P O (F o ld In re s e ) Oxidtive stress ROS formtion Lipid peroxidtion 2.0 2.0 1.5 0.5 1.5 0.5 0.0 0.0 -r d i tio n r te (m G y /h ) -r d i tio n r te (m G y /h ) Ativtion ntioxidnt defense system
M M P (F o ld In re s e ) Oxidtive phosphoryltion ROS 1.2 1.1 Mitohondril memrne potentil 0.9 0.8 0.7 0.6 -r d i tio n r te (m G y /h ) UVR redued MMP nd interfere gmm rdition effet
F v /F m (F o ld In re s e ) E T R (F o ld In re s e ) N P Q (F o ld In re s e ) PS II inhiition RO S 1.1 0.9 0.8 0.7 Mximl PS II effiieny 0 1 2 2 0 4 0 1.1 0.9 0.8 0.7 Eletron trnsport rte 0 1 2 2 0 4 0 2.0 1.5 0.5 0.0 Non-photohemil quenhing d 0 1 2 2 0 4 0 -r d i tio n r te (m G y /h ) -r d i tio n r te (m G y /h ) -r d i tio n r te (m G y /h ) In omined exposure, -rdition nd UV hd dditive effets on Fv/Fm
C h l + (F o ld In re s e ) C ro te n o id s (F o ld In r e s e ) Pigment ontent ROS Totl hlorophyll Totl rotenoids 1.5 2.0 1.5 0.5 0.5 0.0 0.0 -r d i tio n r te (m G y /h ) -r d i tio n r te (m G y /h ) Crotenoids re lso ntioxidnts to quenhing ROS in plnt
? Pigment destrution PSII effiieny Growth NPQ inrese ETR loking ROS PSII effiieny Growth Popultion trjetories OXPHOS Stressor Moleulr inititing Event Key Events Individul effet Popultion effet
Tâni Gomes You Song Dg Brede Dg Wenner Hns Christin Teien Yetneerk Aylew Kssye Terje Christensen http://footge.frmepool.om THANK YOU! Li.Xie@niv.no