InertSustainSwift C8

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InertSustainSwift C8

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HPLC, LC/MS Columns InertSustainSwift TM C8 New! InertSustainSwift C8 is an octyl group (C8) bonded column offering the same extreme inertness to any type of compounds just like InertSustainSwift C8, which is ideal for analyzing low polarity analytes. In addition, the optimized 00A pore size silica enables to analyze and retain peptides and oligonucleotides which have a molecular weight from several kda to several dozen kda. Physical Properties Silica Particle Size Surface Area Pore Size Pore Volume Bonded Phase End-capping Carbon Loading ph Range USP Code :ES (Evolved Surface) Silica Gel :.9 μm, μm, μm :00 m /g :00 Å (0 nm) :.00 ml/g :Octyl Groups :Complete :6 % :~0 :L7 Fat-Soluble Vitamins Many chromatographers prefer a C8 column when an ODS phase shows excessive retention values. In this example, the InertSustainSwift C8 provides shorter analysis time with better selectivity on the impurities eluted after sample no. due to the shorter alkyl chain length of the stationary phase. InertSustainSwift C8 0 6 8 0 Conditions Column Size Eluent Now Less than 0 Minutes Without Sacrificing Separation : μm 0.6mm I.D. : A) H O B) CH CN A/B = 0/90,v/v :.0 ml/min Flow Rate Col. Temp : 0 Detection : UV 00 nm Sample :. Retinol (Vitamin A). Cholecalciferol (Vitamin D ). α-tocopherol (Vitamin E). Phylloquinone (Vitamin K ). Impurities of 0 0 0 0 0 0 InertSustainSwift C8 Total Analysis Time 0 minutes 6 8 0 6 8 0 0 0 0 0 0

Efficiency (N) % Initial Retention Time Extreme Resistance to Low and High ph Mobile Phases The most popular way to start method development is to use a buffered low ph mobile phase. Using a low ph mobile phase frequently delivers the best peak shape for basic compounds on silica-based columns. However, low ph solvents tend to cause poor column lifetime due to the acid hydrolysis of the bonded phase. For samples that were not well retained at low or mid ph, the usage of high ph mobile phases is required to achieve satisfactory retention and separation. High ph separations on silica-based columns are generally avoided due to the short column lifetimes. As shown below, InertSustainSwift C8 not only show high resistance to low ph but also to high ph due to the usage of a radically new type of silica, in which the surface of the silica is uniquely modified, enabling precise control of the silica properties. Low ph Resistance Test 0 00 80 60 0 0 0 0 0 00 0 00 0 Hours of Purging (h) InertSustainSwift C8 InertSustain C8 Inertsil C8- Inertsil C8- Conditions Purging Solvent Column Temp. : 60 Sample : Naphthalene : 0. % TFA (ph.0)/ch CN = 90/0, v/v Testing Procedure ) Purging solvent is introduced into column. ) The column is then flushed with 0 % CH CN. ) Naphthalene is used to verify the % initial retention. High ph Resistance Test 0 00 80 Conditions Purging Solvent Column Temp. : 0 Sample : Naphthalene : 0 mm TEA (ph 9.0)/CH OH = 70/0, v/v 60 0 0 0 0 0 0 60 80 00 0 0 60 80 00 Hours of Purging (h) InertSustainSwift C8 InertSustain C8 Inertsil C8- Inertsil C8- Triart C8 Luna C8 Testing Procedure ) Purging solvent is introduced into column. ) The column is then flushed with 0 % CH OH. ) Naphthalene is used to verify the efficiency.

About InertSustainSwift columns InertSustainSwift, the name of our brand comes from the key word INERT, which is the most important factor in the separation analysis. GL Sciences InertsSustainSwift columns deliver the maximum performance of an HPLC column. Reversed phase C8 columns comprise the vast majority of columns used in the HPLC separation industry. We selected a wide variety of popular brands and compared them with GL Sciences InertSustainSwift C8. Comparison Table Column Surface Area (m /g) Pore Size (A ) Pore Volume (ml/g) Carbon Loading (%) ph Range InertSustainSwfit C8 00 00.00 6-0 Hypersil BDS C8 70 0 0.6 7-9 Triart C8-0 - 7 - Luna C8() 00 00 -..-0 YMC-Pack Pro C8 0 0.97 0-7. L-column C8 0 0. 0-7. SunFire C8 0 00 0.86. -8 XBridge C8 8 0 0.77 - Zorbax Eclipse Plus C8 9 60-8 -9 Particle size: all μm Column size: all 0 x.6 mm I.D.

Explanation of Analytical Tests and Conditions (/) By conducting the following tests, we can evaluate the selectivity, degree of inertness of the column and whether it can be used in a 00 % aqueous mobile phase condition. To strictly evaluate all columns, the below mentioned samples were used, which are all known as strong adsorptive challenging compounds. Selectivity Test Sample No., n-butylbenzene and Sample No., n-amylbenzene were used to determine the hydrophobic property of the column. n-amylbenzene elutes later against n-butylbenzene when the hydrophobicity of the column is high. Stereoselectivity is indicated by Sample No.6, o-terphenyl and Sample No.7, Triphenylene. O- Terphenyl has a twisted tertiary structure and Triphenylene has a planar structure. Triphenylene elutes later against o-terphenyl when the stereoselectivity of the column is high. Conditions Eluent : A) CH OH B) H O A/B = 80/0, v / v Flow Rate :.0 ml/min Col. Temp. : 0 Detection : UV nm Sample :. Uracil. Caffeine. Phenol. n-butylbenzene. n-amylbenzene 6.o-Terphenyl 7. Triphenylene. n-butylbenzene. n-amylbenzene 6. o-terphenyl 7. Triphenylene Basic Compound Test () Dextromethorphan and Berberine chloride are strong basic compounds. Severe tailing can be confirmed when the packing material contains residual silanol groups. O O N H CO Conditions Eluent : A) CH CN B) mm K HPO (ph 7.0, KH PO ) A/B = 0/70, v/ v Flow Rate :.0 ml/min Col. Temp. : 0 Detection : UV 0 nm Sample :.Uracil.Pyridine.Phenol.Berberine chloride.dextromethorphan OCH H N CH OCH. Berberine chloride. Dextromethorphan Basic Compound Test () A basic compound test using antihistamine drugs and are highly basic, which can show tailing of peaks and different elution pattern on columns with insufficient end-capping. Column with insufficient end-capping will show later elution of sample,, and. Conditions Eluent : A) CH CN B) mm K HPO (ph 7.0, KH PO ) A/B = 60/0, v / v :.0 ml/min Flow Rate Col. Temp. : 0 Detection : UV 0 nm Sample :. Chlorpheniramine. Triprolidine. Homochlorcyclizine. Hydroxyzine. Clemastine N N Cl N H C N Cl N H N CH Cl. Chlorpheniramine. Triprolidine. Homochlorcyclizine. Hydroxyzine. Clemastine N H N O OH Cl O CH N H CH

Explanation of Analytical Tests and Conditions (/) Acidic Compound Test Sharp peaks can be obtained when analyzing Phenol or Salicylic Acid. However, as Brilliant Blue FCF has three sulfonic groups in its chemical structure, tailing will occur when the surface of the packing material is slightly basic. - OS - SO SO - Conditions Eluent : A) CH CN B) 0.% H PO A/B = /7, v/v :.0 ml/min Flow Rate Col. Temp. : 0 Detection : UV nm Sample :.Brilliant Blue FCF.Phenol. Salicylic acid N N +. Brilliant Blue FCF Chelating Compound Test Hinokitiol is a strong chelating compound, which coordinately binds with the surface of residual trace metal impurities, resulting in severe tailing. However, the peak shape improves as the injection increases since the surface of the packing material of the adsorption active sites eventually become masked. O OH Conditions Eluent : A) CH CN B) 0.%H PO A/B = 0/60, v/v Flow Rate :.0 ml/min Col. Temp. : 0 Detection : UV nm Inject Vol. : μl, 0ppm Sample :. Phenol. β-thujaplicin (Hinokitiol). β-thujaplicin (Hinokitiol) Dewetting Test When analyzing hydrophilic compounds under water rich mobile phase condition, once the pump is stopped, the hydrophobic bonded group pushes the aqueous mobile phase out off the pore in an irreversible fashion, in what has become known as the dewetting phenomenon. Conditions Eluent: 00 % H O Flow Rate :.0 ml/min Col. Temp. : 0 Detection : UV nm Sample :.Cytosine.Uracil.Guanine.Thymine.Adenine Testing Procedure: ) 00 % water is introduced into column over 60 minutes. ) Conduct Analysis (Upper chromatogram in the following pages) ) Stop flow for minutes. ) 00 % water is introduced again into column over 0 minutes. ) Stop flow for minutes again. 6) Conduct Analysis (Lower chromatogram in the following pages)

Comparison of Performance /8 Selectivity Test Basic Compound Test () Basic Compound Test () InertSustainSwift C8. Uracil. Caffeine. Phenol. n-butylbenzene. n-amylbenzene 6. o-terphenyl 7. Triphenylene.Uracil.Phenol.Dextromethorphan.Pyridine.Berberine chloride. Chlorpheniramine. Triprolidine. Homochlorcyclizine. Hydroxyzine. Clemastine 6 7 0 0 0 0 0 0 0 0 6 8 0 Hypersil BDS C8 6, 7 :Adsorption observed Severe tailing of peaks 0 0 0 0 0 0 0 0 6 8 0 Luna C8() :Adsorption observed Severe tailing of peaks, 7 6 0 0 0 0 0 0 0 0 0 6 8 0

Comparison of Performance /8 Selectivity Test Basic Compound Test () Basic Compound Test () InertSustainSwift C8. Uracil. Caffeine. Phenol. n-butylbenzene. n-amylbenzene 6. o-terphenyl 7. Triphenylene.Uracil.Phenol.Dextromethorphan.Pyridine.Berberine chloride. Chlorpheniramine. Triprolidine. Homochlorcyclizine. Hydroxyzine. Clemastine 6 7 0 0 0 0 0 0 0 0 6 8 0 XBridge C8 7 6 0 0 0 0 0 0 0 0 0 6 8 0 SunFire C8 :Adsorption observed Severe tailing of peaks 6, 7 0 0 0 0 0 0 0 0 0 0 0

Comparison of Performance /8 Selectivity Test Basic Compound Test () Basic Compound Test () InertSustainSwift C8. Uracil. Caffeine. Phenol. n-butylbenzene. n-amylbenzene 6. o-terphenyl 7. Triphenylene.Uracil.Phenol.Dextromethorphan.Pyridine.Berberine chloride. Chlorpheniramine. Triprolidine. Homochlorcyclizine. Hydroxyzine. Clemastine 6 7 0 0 0 0 0 0 0 0 6 8 0 ZORBAX Eclipse Plus C8 7 :Adsorption observed Tailing of peaks 6 0 0 0 0 0 0 0 0 0 0 0 Triart C8 7 6 0 0 0 0 0 0 0 0 6 8 0

Comparison of Performance /8 Selectivity Test Basic Compound Test () Basic Compound Test () InertSustainSwift C8. Uracil. Caffeine. Phenol. n-butylbenzene. n-amylbenzene 6. o-terphenyl 7. Triphenylene.Uracil.Phenol.Dextromethorphan.Pyridine.Berberine chloride. Chlorpheniramine. Triprolidine. Homochlorcyclizine. Hydroxyzine. Clemastine 6 7 0 0 0 0 0 0 0 0 6 8 0 YMC-Pack Pro C8 :Adsorption observed Severe tailing of peaks 7 6 0 0 0 0 0 0 0 0 0 0 0 L-column C8, :Severe tailing of peaks 6 7 0 0 0 0 0 0 0 0 6 8 0

Comparison of Performance /8 Acidic Compound Test Chelating Compound Test Dewetting Test InertSustainSwift C8.Brilliant Blue FCF.Salicylic acid.phenol. Phenol. β-thujaplicin (Hinokitiol).Cytosine.Uracil.Guanine.Thymine.Adenine 0 0 0 Variation: 99. % 0 0 0 0 0 0 0 0 0 0 Hypersil BDS C8 :Tailing of peak 0 0 0 Variation: 7. % 0 0 0 0 0 0 0 0 0 0 Luna C8() :Adsorption observed 0 0 0 Variation: 9. % 0 0 0 0 0 0 0 0 0 0

Comparison of Performance 6/8 Acidic Compound Test Chelating Compound Test Dewetting Test InertSustainSwift C8.Brilliant Blue FCF.Salicylic acid.phenol. Phenol. β-thujaplicin (Hinokitiol).Cytosine.Guanine.Adenine.Uracil.Thymine 0 0 0 Variation: 99. % 0 0 0 0 0 0 0 0 0 0 XBridge C8 :Not eluted :Adsorption observed 0 0 0 Variation: 66.8 % 0 0 0 0 0 0 0 0 0 0 SunFire C8 :Not eluted :Adsorption observed 0 0 0 Variation: 8. % 0 0 0 0 0 0 0 0 0 0

Comparison of Performance 7/8 Acidic Compound Test Chelating Compound Test Dewetting Test InertSustainSwift C8.Brilliant Blue FCF.Salicylic acid.phenol. Phenol. β-thujaplicin (Hinokitiol).Cytosine.Guanine.Adenine.Uracil.Thymine 0 0 0 Variation: 99. % 0 0 0 0 0 0 0 0 0 0 ZORBAX Eclipse Plus C8 :Adsorption observed :Adsorption observed 0 0 0 Variation: 8. % 0 0 0 0 0 0 0 0 0 0 Triart C8 0 0 0 Variation: 89.9 % 0 0 0 0 0 0 0 0 0 0

Comparison of Performance 8/8 Acidic Compound Test Chelating Compound Test Dewetting Test InertSustainSwift C8.Brilliant Blue FCF.Salicylic acid.phenol. Phenol. β-thujaplicin (Hinokitiol).Cytosine.Uracil.Guanine.Thymine.Adenine 0 0 0 Variation: 99. % 0 0 0 0 0 0 0 0 0 0 YMC-Pack Pro C8 :Not eluted :Severe tailing of peak 0 0 0 Variation: 6. % 0 0 0 0 0 0 0 0 0 0 L-column C8 :Severe fronting of peak 0 0 0 Variation: 0. % 0 0 0 0 0 0 0 0 0 0

Analytical Columns Cat No. 00-88 InertSustainSwift C8.9 μm.x0mm 80 00-88 InertSustainSwift C8.9 μm.x00mm 80 00-88 InertSustainSwift C8.9 μm.x0mm 80 00-886 InertSustainSwift C8.9 μm.0x0mm 80 00-887 InertSustainSwift C8.9 μm.0x00mm 80 00-888 InertSustainSwift C8.9 μm.0x0mm 80 00-88 InertSustainSwift C8 HP μm.x0mm 0 00-886 InertSustainSwift C8 HP μm.x00mm 0 00-887 InertSustainSwift C8 HP μm.x0mm 0 00-888 InertSustainSwift C8 HP μm.x0mm 0 00-889 InertSustainSwift C8 HP μm.0x0mm 0 00-880 InertSustainSwift C8 HP μm.0x00mm 0 00-88 InertSustainSwift C8 HP μm.0x0mm 0 00-88 InertSustainSwift C8 HP μm.0x0mm 0 00-88 InertSustainSwift C8 HP μm.6x0mm 0 00-88 InertSustainSwift C8 HP μm.6x00mm 0 00-88 InertSustainSwift C8 HP μm.6x0mm 0 00-886 InertSustainSwift C8 HP μm.6x0mm 0 * End-fittings are /6 Parker style.

Analytical Columns Cat No. 00-8866 InertSustainSwift C8 μm.0x 0mm 0 00-8867 InertSustainSwift C8 μm.0x 0mm 0 00-8868 InertSustainSwift C8 μm.0x 7mm 0 00-8869 InertSustainSwift C8 μm.0x00mm 0 00-8870 InertSustainSwift C8 μm.0x0mm 0 00-887 InertSustainSwift C8 μm.0x0mm 0 00-887 InertSustainSwift C8 μm.x 0mm 0 00-887 InertSustainSwift C8 μm.x 0mm 0 00-887 InertSustainSwift C8 μm.x 7mm 0 00-887 InertSustainSwift C8 μm.x00mm 0 00-8876 InertSustainSwift C8 μm.x0mm 0 00-8877 InertSustainSwift C8 μm.x0mm 0 00-886 InertSustainSwift C8 μm.x 0mm 0 00-887 InertSustainSwift C8 μm.x 0mm 0 00-888 InertSustainSwift C8 μm.x 7mm 0 00-889 InertSustainSwift C8 μm.x 00mm 0 00-880 InertSustainSwift C8 μm.xmm 0 00-88 InertSustainSwift C8 μm.x0mm 0 00-88 InertSustainSwift C8 μm.x0mm 0 00-88 InertSustainSwift C8 μm.0x 0mm 0 00-88 InertSustainSwift C8 μm.0x 0mm 0 00-886 InertSustainSwift C8 μm.0x 7mm 0 00-887 InertSustainSwift C8 μm.0x00mm 0 00-888 InertSustainSwift C8 μm.0xmm 0 00-889 InertSustainSwift C8 μm.0x0mm 0 00-880 InertSustainSwift C8 μm.0x0mm 0 00-88 InertSustainSwift C8 μm.0x 0mm 0 00-88 InertSustainSwift C8 μm.0x 0mm 0 00-88 InertSustainSwift C8 μm.0x 7mm 0 00-88 InertSustainSwift C8 μm.0x00mm 0 00-886 InertSustainSwift C8 μm.0xmm 0 00-887 InertSustainSwift C8 μm.0x0mm 0 00-888 InertSustainSwift C8 μm.0x0mm 0 00-880 InertSustainSwift C8 μm.6x 0mm 0 00-88 InertSustainSwift C8 μm.6x 0mm 0 00-88 InertSustainSwift C8 μm.6x 7mm 0 00-88 InertSustainSwift C8 μm.6x00mm 0 00-88 InertSustainSwift C8 μm.6xmm 0 00-88 InertSustainSwift C8 μm.6x0mm 0 00-886 InertSustainSwift C8 μm.6x0mm 0 * End-fittings are /6 Waters-compatible.

Analytical Columns Cat No. 00-88 InertSustainSwift C8 μm.0x 0mm 0 00-88 InertSustainSwift C8 μm.0x 0mm 0 00-88 InertSustainSwift C8 μm.0x 7mm 0 00-88 InertSustainSwift C8 μm.0x00mm 0 00-886 InertSustainSwift C8 μm.0x0mm 0 00-887 InertSustainSwift C8 μm.0x0mm 0 00-888 InertSustainSwift C8 μm.x 0mm 0 00-889 InertSustainSwift C8 μm.x 0mm 0 00-880 InertSustainSwift C8 μm.x 7mm 0 00-88 InertSustainSwift C8 μm.x00mm 0 00-88 InertSustainSwift C8 μm.x0mm 0 00-88 InertSustainSwift C8 μm.x0mm 0 00-880 InertSustainSwift C8 μm.x 0mm 0 00-880 InertSustainSwift C8 μm.x 0mm 0 00-880 InertSustainSwift C8 μm.x 7mm 0 00-880 InertSustainSwift C8 μm.x00mm 0 00-8806 InertSustainSwift C8 μm.xmm 0 00-8807 InertSustainSwift C8 μm.x0mm 0 00-8808 InertSustainSwift C8 μm.x0mm 0 00-880 InertSustainSwift C8 μm.0x 0mm 0 00-88 InertSustainSwift C8 μm.0x 0mm 0 00-88 InertSustainSwift C8 μm.0x 7mm 0 00-88 InertSustainSwift C8 μm.0x00mm 0 00-88 InertSustainSwift C8 μm.0xmm 0 00-88 InertSustainSwift C8 μm.0x0mm 0 00-886 InertSustainSwift C8 μm.0x0mm 0 00-888 InertSustainSwift C8 μm.0x 0mm 0 00-889 InertSustainSwift C8 μm.0x 0mm 0 00-880 InertSustainSwift C8 μm.0x 7mm 0 00-88 InertSustainSwift C8 μm.0x00mm 0 00-88 InertSustainSwift C8 μm.0xmm 0 00-88 InertSustainSwift C8 μm.0x0mm 0 00-88 InertSustainSwift C8 μm.0x0mm 0 00-886 InertSustainSwift C8 μm.6x 0mm 0 00-887 InertSustainSwift C8 μm.6x 0mm 0 00-888 InertSustainSwift C8 μm.6x 7mm 0 00-889 InertSustainSwift C8 μm.6x00mm 0 00-880 InertSustainSwift C8 μm.6xmm 0 00-88 InertSustainSwift C8 μm.6x0mm 0 00-88 InertSustainSwift C8 μm.6x0mm 0 * End-fittings are /6 Waters-compatible.

Guard Columns for UHPLC Cat No. 00-0860 Holder for Guard Columns for UHPLC - Guard Columns for UHPLC, Holder/Cartridge Set, 00-88 Holder with pcs.x0 mm Cartridges, InertSustainSwift C8.9 μm 80 Guard Columns for UHPLC, Holder/Cartridge Set, 00-88 Holder with pcs.x0 mm Cartridges, InertSustainSwift C8.9 μm 80 Guard Columns for UHPLC, Holder/Cartridge Set, 00-88 Holder with pcs.0x0 mm Cartridges, InertSustainSwift C8.9 μm 80 Guard Columns for UHPLC, Holder/Cartridge Set, 00-880 Holder with pcs.x0 mm Cartridges, InertSustainSwift C8 μm 80 Guard Columns for UHPLC, Holder/Cartridge Set, 00-88 Holder with pcs.x0 mm Cartridges, InertSustainSwift C8 μm 80 Guard Columns for UHPLC, Holder/Cartridge Set, 00-88 Holder with pcs.0x0 mm Cartridges, InertSustainSwift C8 μm 80 00-889 Guard Columns for UHPLC,.x0 mm, pcs, InertSustainSwift C8.9 μm 80 00-880 Guard Columns for UHPLC,.x0 mm, pcs, InertSustainSwift C8.9 μm 80 00-88 Guard Columns for UHPLC,.0x0 mm, pcs, InertSustainSwift C8.9 μm 80 00-887 Guard Columns for UHPLC,.x0 mm, pcs, InertSustainSwift C8 μm 80 00-888 Guard Columns for UHPLC,.x0 mm, pcs, InertSustainSwift C8 μm 80 00-889 Guard Columns for UHPLC,.0x0 mm, pcs, InertSustainSwift C8 μm 80 Cartridge Guard Column Ei (Non-Metal) Cat# 00-88 Holder for 0 mm, Cartridge Guard Column Ei - 00-880 Cartridge Ei Holder/Cartridge Set, Holder with pcs.0x0 mm Cartridges, InertSustainSwift C8 µm 0 00-888 Cartridge Ei Holder/Cartridge Set, Holder with pcs.x0 mm Cartridges, InertSustainSwift C8 µm 0 00-88 Cartridge Ei Holder/Cartridge Set, Holder with pcs.0x0 mm Cartridges, InertSustainSwift C8 µm 0 00-889 Cartridge EiReplacement Cartridges, pcs.0x0mm, InertSustainSwift C8 µm 0 00-887 Cartridge EiReplacement Cartridges, pcs.x0mm, InertSustainSwift C8 µm 0 00-88 Cartridge EiReplacement Cartridges, pcs.0x0mm, InertSustainSwift C8 µm 0 * End-fittings are /6 Waters-compatible.

Cartridge Guard Column E Cat No. * End-fittings are /6 Waters-compatible. 00-0800 Holder for 0 mm, Cartridge Guard Column E - 00-080 Holder for 0 mm, Cartridge Guard Column E - 00-8806 Holder with pcs.0x0 mm Cartridges, InertSustainSwift C8 µm 0 00-8808 Holder with pcs.x0 mm Cartridges, InertSustainSwift C8 µm 0 00-880 Holder with pcs.0x0 mm Cartridges, InertSustainSwift C8 µm 0 00-880 Holder with pcs.0x0 mm Cartridges, InertSustainSwift C8 µm 0 00-88 Holder with pcs.0x0 mm Cartridges, InertSustainSwift C8 µm 0 00-880 Holder with pcs.0x0 mm Cartridges, InertSustainSwift C8 µm 0 00-880 Holder with pcs.0x0 mm Cartridges, InertSustainSwift C8 µm 0 00-88 Holder with pcs.x0 mm Cartridges, InertSustainSwift C8 µm 0 00-8808 Holder with pcs.0x0 mm Cartridges, InertSustainSwift C8 µm 0 00-8806 Holder with pcs.0x0 mm Cartridges, InertSustainSwift C8 µm 0 00-886 Holder with pcs.0x0 mm Cartridges, InertSustainSwift C8 µm 0 00-88 Holder with pcs.0x0 mm Cartridges, InertSustainSwift C8 µm 0 00-880 Cartridge E Replacement Cartridges, pcs.0x0mm, InertSustainSwift C8 µm 0 00-8807 Cartridge E Replacement Cartridges, pcs.x0mm, InertSustainSwift C8 µm 0 00-880 Cartridge E Replacement Cartridges, pcs.0x0mm, InertSustainSwift C8 µm 0 00-88 Cartridge E Replacement Cartridges, pcs.0x0mm, InertSustainSwift C8 µm 0 00-880 Cartridge E Replacement Cartridges, pcs.0x0mm, InertSustainSwift C8 µm 0 00-8809 Cartridge E Replacement Cartridges, pcs.0x0mm, InertSustainSwift C8 µm 0 00-8809 Cartridge E Replacement Cartridges, pcs.0x0mm, InertSustainSwift C8 µm 0 00-88 Cartridge E Replacement Cartridges, pcs.x0mm, InertSustainSwift C8 µm 0 00-8807 Cartridge E Replacement Cartridges, pcs.0x0mm, InertSustainSwift C8 µm 0 00-880 Cartridge E Replacement Cartridges, pcs.0x0mm, InertSustainSwift C8 µm 0 00-88 Cartridge E Replacement Cartridges, pcs.0x0mm, InertSustainSwift C8 µm 0 00-88 Cartridge E Replacement Cartridges, pcs.0x0mm, InertSustainSwift C8 µm 0

GL Cart Guard Columns Cat No. 00-0870 Holder for GL Cart Multipurpose Type - 00-88 GL Cart Holder/Cartridge Set, Holder with pcs.6x mm Cartridges, InertSustainSwift C8 µm 0 00-88 GL Cart Replacement Cartridges, 0 pcs.6x mm, InertSustainSwift C8 µm 0 00-88 GL Cart Holder/Cartridge Set, Holder with pcs.6x mm Cartridges, InertSustainSwift C8 µm 0 00-88 GL Cart Replacement Cartridges, 0 pcs.6x mm, InertSustainSwift C8 µm 0 * End-fittings are /6 Waters-compatible. Conventional Guard Columns Cat No. 00-888 Guard Column.0xmm InertSustainSwift C8 µm 0 00-8879 Guard Column.0x0mm InertSustainSwift C8 µm 0 00-8880 Guard Column.xmm InertSustainSwift C8 µm 0 00-8878 Guard Column.x0mm InertSustainSwift C8 µm 0 00-886 Guard Column.xmm InertSustainSwift C8 µm 0 00-8860 Guard Column.x0mm InertSustainSwift C8 µm 0 00-886 Guard Column.0xmm InertSustainSwift C8 µm 0 00-889 Guard Column.0x0mm InertSustainSwift C8 µm 0 00-886 Guard Column.0x0mm InertSustainSwift C8 µm 0 00-886 Guard Column.0xmm InertSustainSwift C8 µm 0 00-888 Guard Column.0x0mm InertSustainSwift C8 µm 0 00-886 Guard Column.6xmm InertSustainSwift C8 µm 0 00-887 Guard Column.6x0mm InertSustainSwift C8 µm 0 00-887 Guard Column.0xmm InertSustainSwift C8 µm 0 00-88 Guard Column.0x0mm InertSustainSwift C8 µm 0 00-886 Guard Column.xmm InertSustainSwift C8 µm 0 00-88 Guard Column.x0mm InertSustainSwift C8 µm 0 00-88 Guard Column.xmm InertSustainSwift C8 µm 0 00-886 Guard Column.x0mm InertSustainSwift C8 µm 0 00-880 Guard Column.0xmm InertSustainSwift C8 µm 0 00-88 Guard Column.0x0mm InertSustainSwift C8 µm 0 00-887 Guard Column.0x0mm InertSustainSwift C8 µm 0 00-889 Guard Column.0xmm InertSustainSwift C8 µm 0 00-88 Guard Column.0x0mm InertSustainSwift C8 µm 0 00-888 Guard Column.6xmm InertSustainSwift C8 µm 0 00-88 Guard Column.6x0mm InertSustainSwift C8 µm 0 * End-fittings are /6 Waters-compatible.

Preparative Columns Cat No. 00-888 InertSustainSwift C8 µm 6.0x 0mm 0 00-889 InertSustainSwift C8 µm 6.0x00mm 0 00-8860 InertSustainSwift C8 µm 6.0x0mm 0 00-886 InertSustainSwift C8 µm 6.0x0mm 0 00-886 InertSustainSwift C8 µm 7.6x 0mm 0 00-886 InertSustainSwift C8 µm 7.6x00mm 0 00-886 InertSustainSwift C8 µm 7.6x0mm 0 00-886 InertSustainSwift C8 µm 7.6x0mm 0 00-8866 InertSustainSwift C8 µm 0x 0mm 0 00-8867 InertSustainSwift C8 µm 0x00mm 0 00-8868 InertSustainSwift C8 µm 0x0mm 0 00-8869 InertSustainSwift C8 µm 0x0mm 0 00-8870 InertSustainSwift C8 µm x 0mm 0 00-887 InertSustainSwift C8 µm x00mm 0 00-887 InertSustainSwift C8 µm x0mm 0 00-887 InertSustainSwift C8 µm x0mm 0 00-887 InertSustainSwift C8 µm 0x 0mm 0 00-887 InertSustainSwift C8 µm 0x00mm 0 00-8876 InertSustainSwift C8 µm 0x0mm 0 00-8877 InertSustainSwift C8 µm 0x0mm 0 * End-fittings are /6 Waters-compatible. Preparative Columns Cat No. 00-8878 InertSustainSwift C8 µm 6.0x 0mm Guard 0 00-8879 InertSustainSwift C8 µm 7.6x 0mm Guard 0 00-8880 InertSustainSwift C8 µm 0x 0mm Guard 0 00-888 InertSustainSwift C8 µm x 0mm Guard 0 00-888 InertSustainSwift C8 µm 0x 0mm Guard 0 * End-fittings are /6 Waters-compatible. Cartridge Guard Columns for Preparative Columns Cat No. 00-0690 Holder for Prep Guard Cartridge - 00-88 Prep Guard Cartridges, pcs, 7.6x0 mm, InertSustainSwift C8 µm 0 00-88 Prep Guard Holder/Cartridge Set, Holder with pcs 7.6x0 mm Cartridges, InertSustainSwift C8 µm 0

Capillary Columns Cat No. 00-8889 Capillary EX-NANO 0.0x 0mm InertSustainSwift C8 µm 00-8890 Capillary EX-NANO 0.0x0mm InertSustainSwift C8 µm 00-889 Capillary EX-NANO 0.0x0mm InertSustainSwift C8 µm 00-889 Capillary EX-NANO 0.07x 0mm InertSustainSwift C8 µm 00-889 Capillary EX-NANO 0.07x0mm InertSustainSwift C8 µm 00-889 Capillary EX-NANO 0.07x0mm InertSustainSwift C8 µm 00-889 Capillary EX-NANO 0.x 0mm InertSustainSwift C8 µm 00-8896 Capillary EX-NANO 0.x0mm InertSustainSwift C8 µm 00-8897 Capillary EX-NANO 0.x0mm InertSustainSwift C8 µm 00-8898 Capillary EX-NANO 0.x 0mm InertSustainSwift C8 µm 00-8899 Capillary EX-NANO 0.x0mm InertSustainSwift C8 µm 00-8800 Capillary EX-NANO 0.x0mm InertSustainSwift C8 µm 00-888 Capillary EX 0.x 0mm InertSustainSwift C8 µm 0 00-888 Capillary EX 0.x0mm InertSustainSwift C8 µm 0 00-888 Capillary EX 0.x 0mm InertSustainSwift C8 µm 0 00-888 Capillary EX 0.x0mm InertSustainSwift C8 µm 0 00-8886 Capillary EX 0.7x 0mm InertSustainSwift C8 µm 0 00-8887 Capillary EX 0.7x0mm InertSustainSwift C8 µm 0 00-8888 EX Micro Guard (Metal) 0.xmm InertSustainSwift C8 µm 0 00-889 Capillary EX-NANO 0.0x 0mm InertSustainSwift C8 µm 00-889 Capillary EX-NANO 0.0x0mm InertSustainSwift C8 µm 00-889 Capillary EX-NANO 0.0x0mm InertSustainSwift C8 µm 00-8896 Capillary EX-NANO 0.07x 0mm InertSustainSwift C8 µm 00-8897 Capillary EX-NANO 0.07x0mm InertSustainSwift C8 µm 00-8898 Capillary EX-NANO 0.07x0mm InertSustainSwift C8 µm 00-8899 Capillary EX-NANO 0.x 0mm InertSustainSwift C8 µm 00-8800 Capillary EX-NANO 0.x0mm InertSustainSwift C8 µm 00-880 Capillary EX-NANO 0.x0mm InertSustainSwift C8 µm 00-880 Capillary EX-NANO 0.x 0mm InertSustainSwift C8 µm 00-880 Capillary EX-NANO 0.x0mm InertSustainSwift C8 µm 00-880 Capillary EX-NANO 0.x0mm InertSustainSwift C8 µm 00-888 Capillary EX 0.x 0mm InertSustainSwift C8 µm 0 00-8886 Capillary EX 0.x0mm InertSustainSwift C8 µm 0 00-8887 Capillary EX 0.x 0mm InertSustainSwift C8 µm 0 00-8888 Capillary EX 0.x0mm InertSustainSwift C8 µm 0 00-8889 Capillary EX 0.7x 0mm InertSustainSwift C8 µm 0 00-8890 Capillary EX 0.7x0mm InertSustainSwift C8 µm 0 00-889 EX Micro Guard (Metal) 0.xmm InertSustainSwift C8 µm 0 00-889 EX Micro Guard (Non-Metal) 0.xmm InertSustainSwift C8 µm 0 * End-fittings are Valco /6 (0- UNF). * Valco / (6-0 UNF) end-fittings are available, indicate / when ordering.

InertSearch Application Notes Access to the latest pharmaceutical, life science, environmental and food applications at www.glsciences.com/tech/inertsearch Worldwide Ordering Information GL Sciences, Inc. USA 7 Torrance Blvd. Suite Torrance, CA 900 Phone: 0-6- Fax: 0-6- Email: info@glsciencesinc.com Web: www.glsciencesinc.com International Distributors Visit our Website at www.glsciences.com/distributors GL Sciences B.V. De Sleutel 9 6 AS Eindhoven The Netherlands Phone: + (0)0 9 Email: info@glsciences.eu Web: www.glsciences.eu GL Sciences, Inc. Japan - Nishishinjuku 6-Chome Shinjuku-ku, Tokyo, 6-0, Japan Phone: +8---660 Fax: +8---66 Email: world@gls.co.jp Web: www.glsciences.com The GL Sciences name, the GL Sciences logo and the following registered trademark or trademark are the property of GL Sciences Inc. InertSustainSwift InertSustain Inertsil All other trademarks or service marks are the property of their respective owners. The specification and the column type are subject to change without notice due to continual improvements.