LABORATORY REPORT If you have any questions concerning this report, please do not hesitate to call us at (800) 332-4345 or (574) 233-4777. This report may not be reproduced, except in full, without written approval from EEA. Page 1 of 13
STATE CERTIFICATION LIST State Certification State Certification Alabama 40700 Missouri 880 Alaska IN00035 Montana CERT0026 Arizona AZ0432 Nebraska NE-OS-05-04 Arkansas IN00035 Nevada IN00035 California 2920 New Hampshire* 2124 Colorado IN035 New Jersey* IN598 Colorado Radiochemistry IN035 New Mexico IN00035 Connecticut PH-0132 New York* 11398 Delaware IN035 North Carolina 18700 Florida* E87775 North Dakota R-035 Georgia 929 Ohio 87775 Hawaii IN035 Oklahoma D9508 Idaho IN00035 Oregon (Primary AB)* 4074-001 Illinois* 200001 Pennsylvania* 68-00466 Illinois Microbiology 17767 Puerto Rico IN00035 Illinois Radiochemistry IN00035 Rhode Island LAO00343 Indiana Chemistry C-71-01 South Carolina 95005 Indiana Microbiology M-76-07 South Dakota IN00035 Iowa 098 Tennessee TN02973 Kansas* E-10233 Texas* T104704187-15-8 Kentucky 90056 Texas/TCEQ TX207 Louisiana* LA180008 Utah* IN00035 Maine IN00035 Vermont VT-8775 Maryland 209 Virginia* 460275 Massachusetts M-IN035 Washington C837 Michigan 9926 West Virginia 9927 C Minnesota* 018-999-338 Wisconsin 999766900 Mississippi IN035 Wyoming IN035 EPA IN00035 *NELAP/TNI Recognized Accreditation Bodies Revision date: 01/02/2018 Page 2 of 13
110 South Hill Street South Bend, IN 46617 Tel: (574) 233-4777 Fax: (574) 233-8207 1 800 332 4345 Laboratory Report Client: Hillsborough County Board of County Commissioners Report: 426737 Attn: PWS Facility ID: Sampling Point ID: Ed Watson 332 N. Falkenburd Rd. Tampa, FL 33619 Sample Event Code: EEA ID # 00002 0787002 SE8 Sample Information Priority: Status: Lab ID Code: PWS ID: Client ID Method Collected Date / Time Standard Written Final IN00035 FL6290787 Collected By: Received Date / Time 4028692 0787002 Central 545 08/20/18 08:49 Client 08/21/18 10:00 4028693 0787002 Central 546 08/20/18 08:49 Client 08/21/18 10:00 Report Summary Detailed quantitative results are presented on the following pages. The results presented relate only to the samples provided for analysis. We appreciate the opportunity to provide you with this analysis. If you have any questions concerning this report, please do not hesitate to call Jessie Brasch at (574) 233-4777. Note: This report may not be reproduced, except in full, without written approval from EEA. 09/23/2018 Authorized Signature Title Date Client Name: Report #: Hillsborough County Board of County Commissioners 426737 Page 1 of 2 Page 3 of 13
Client Name: Hillsborough County Board of County Commissioners Report #: 426737 Sampling Point ID: 0787002 PWS ID: FL6290787 Analyte ID # UCMR Result Summary Analyte Method MRL Result Units Preparation Date Analyzed Date 3311 Anatoxin-a 545 0.0300 < 0.0300 ug/l 08/22/18 14:25 08/24/18 20:51 4028692 3302 Cylindrospermopsin 545 0.0900 < 0.0900 ug/l 08/22/18 14:25 08/24/18 20:51 4028692 3301 Total Microcystins & Nodularins 546 0.300 < 0.300 ug/l 08/31/18 12:38 08/31/18 13:16 4028693 CVTM Percent CV 546 N/A 1.30 % 08/31/18 12:38 08/31/18 13:16 4028693 EEA has demonstrated it can achieve these report limits in reagent water, but can not document them in all sample matrices. EEA ID # Page 2 of 2 Page 4 of 13
Lab Definitions Continuing Calibration Check Standard (CCC) / Continuing Calibration Verification (CCV) / Initial Calibration Verification Standard (ICV) / Initial Performance Check (IPC) - is a standard containing one or more of the target analytes that is prepared from the same standards used to calibrate the instrument. This standard is used to verify the calibration curve at the beginning of each analytical sequence, and may also be analyzed throughout and at the end of the sequence. The concentration of continuing standards may be varied, when prescribed by the reference method, so that the range of the calibration curve is verified on a regular basis. CCL, CCM, and CCH are the CCC standards at low, mid, and high concentration levels, respectively. Internal Standards (IS) - are pure compounds with properties similar to the analytes of interest, which are added to field samples or extracts, calibration standards, and quality control standards at a known concentration. They are used to measure the relative responses of the analytes of interest and surrogates in the sample, calibration standard or quality control standard. Laboratory Duplicate (LD) - is a field sample aliquot taken from the same sample container in the laboratory and analyzed separately using identical procedures. Analysis of laboratory duplicates provides a measure of the precision of the laboratory procedures. Laboratory Fortified Blank (LFB) / Laboratory Control Sample (LCS) - is an aliquot of reagent water to which known concentrations of the analytes of interest are added. The LFB is analyzed exactly the same as the field samples. LFBs are used to determine whether the method is in control. FBL, FBM, and FBH are the LFB samples at low, mid, and high concentration levels, respectively. Laboratory Method Blank (LMB) / Laboratory Reagent Blank (LRB) - is a sample of reagent water included in the sample batch analyzed in the same way as the associated field samples. The LMB is used to determine if method analytes or other background contamination have been introduced during the preparation or analytical procedure. The LMB is analyzed exactly the same as the field samples. Laboratory Trip Blank (LTB) / Field Reagent Blank (FRB) - is a sample of laboratory reagent water placed in a sample container in the laboratory and treated as a field sample, including storage, preservation, and all analytical procedures. The FRB/LTB container follows the collection bottles to and from the collection site, but the FRB/LTB is not opened at any time during the trip. The FRB/LTB is primarily a travel blank used to verify that the samples were not contaminated during shipment. Matrix Spike Duplicate Sample (MSD) / Laboratory Fortified Sample Matrix Duplicate (LFSMD) - is a sample aliquot taken from the same field sample source as the Matrix Spike Sample to which known quantities of the analytes of interest are added in the laboratory. The MSD is analyzed exactly the same as the field samples. Analysis of the MSD provides a measure of the precision of the laboratory procedures in a specific matrix. SDL, SDM, and SDH / LFSMDL, LFSMDM, and LFSMDH are the MSD or LFSMD at low, mid, and high concentration levels, respectively. Matrix Spike Sample (MS) / Laboratory Fortified Sample Matrix (LFSM) - is a sample aliquot taken from field sample source to which known quantities of the analytes of interest are added in the laboratory. The MS is analyzed exactly the same as the field samples. The purpose is to demonstrate recovery of the analytes from a sample matrix to determine if the specific matrix contributes bias to the analytical results. MSL, MSM, and MSH / LFSML, LFSMM, and LFSMH are the MS or LFSM at low, mid, and high concentration levels, respectively. Quality Control Standard (QCS) / Second Source Calibration Verification (SSCV) - is a solution containing known concentrations of the analytes of interest prepared from a source different from the source of the calibration standards. The solution is obtained from a second manufacturer or lot if the lot can be demonstrated by the manufacturer as prepared independently from other lots. The QCS sample is analyzed using the same procedures as field samples. The QCS is used as a check on the calibration standards used in the method on a routine basis. Reporting Limit Check (RLC) / Initial Calibration Check Standard (ICCS) - is a procedural standard that is analyzed each day to evaluate instrument performance at or below the minimum reporting limit (MRL). Surrogate Standard (SS) / Surrogate Analyte (SUR) - is a pure compound with properties similar to the analytes of interest, which is highly unlikely to be found in any field sample, that is added to the field samples, calibration standards, blanks and quality control standards before sample preparation. The SS is used to evaluate the efficiency of the sample preparation process. Page 1 of 1 Page 5 of 13
110 South Hill Street South Bend, IN 46617 Tel: (574) 233-4777 Fax: (574) 233-8207 1 800 332 4345 Laboratory Report Client: Attn: Hillsborough County Board of County Commission Ed Watson 332 N. Falkenburd Rd. Tampa, FL 33619 Report: Priority: Status: PWS ID: 426737 Standard Written Final FL6290787 EEA ID # Sample Information Client ID Method Collected Date / Time Collected By: Received Date / Time 4028691 0787002 Central 544 08/20/18 08:49 Client 08/21/18 10:00 Report Summary Method 544 analysis will be performed only if the associated Screening Microcystin by EPA 546 sample result is >=0.3 ug/l. We appreciate the opportunity to provide you with this analysis. If you have any questions concerning this report, please do not hesitate to call Jessie Brasch at (574) 233-4777. Note: This report may not be reproduced, except in full, without written approval from EEA. 09/23/2018 Authorized Signature Title Date Page 1 of 1 Page 6 of 13
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Eurofins Eaton Analytical Run Log Run ID: 247766 Method: 545 0.00 Type Sample Id Sample Site Matrix Instrument ID Analysis Date Calibration File CCL 4029745 RW CY 08/24/2018 18:25 082418M545b-CY.mdb QCS 4029746 RW CY 08/24/2018 18:39 082418M545b-CY.mdb LRB 4029776 RW CY 08/24/2018 18:54 082418M545b-CY.mdb FS 4028692 0787002 Central DW CY 08/24/2018 20:51 082418M545b-CY.mdb CCM 4029747 RW CY 08/24/2018 21:35 082418M545b-CY.mdb Page 8 of 13 Page 1 of 2 EEA Run ID 247766 / EEA Report # 426737
QC Summary Report Sample Type Analyte Method MRL Client ID Result Amount Target Units % Recovery RPD RPD Dil Extracted Analyzed Flag Recovery Limits Limit Factor EEA ID # CCL IS-L-phenylalanine-d5 545 N/A --- 18229 20063 ug/l 91 50-150 --- --- 1.0 08/21/2018 13:35 08/24/2018 18:25 4029745 CCL IS-Uracil-d4 545 N/A --- 5538 5686 ug/l 97 50-150 --- --- 1.0 08/21/2018 13:35 08/24/2018 18:25 4029745 CCL Anatoxin-a 545 0.0300 --- 0.032416 0.03 ug/l 108 50-150 --- --- 1.0 08/21/2018 13:35 08/24/2018 18:25 4029745 CCL Cylindrospermopsin 545 0.0900 --- 0.091938 0.09 ug/l 102 50-150 --- --- 1.0 08/21/2018 13:35 08/24/2018 18:25 4029745 QCS IS-L-phenylalanine-d5 545 N/A --- 18177 20063 ug/l 91 50-150 --- --- 1.0 08/21/2018 13:35 08/24/2018 18:39 4029746 QCS IS-Uracil-d4 545 N/A --- 5560 5686 ug/l 98 50-150 --- --- 1.0 08/21/2018 13:35 08/24/2018 18:39 4029746 QCS Anatoxin-a 545 0.0300 --- 0.576345 0.5 ug/l 115 70-130 --- --- 1.0 08/21/2018 13:35 08/24/2018 18:39 4029746 QCS Cylindrospermopsin 545 0.0900 --- 1.50927 1.5 ug/l 101 70-130 --- --- 1.0 08/21/2018 13:35 08/24/2018 18:39 4029746 LRB IS-L-phenylalanine-d5 545 N/A --- 18035 20063 ug/l 90 50-150 --- --- 1.0 08/22/2018 14:25 08/24/2018 18:54 4029776 LRB IS-Uracil-d4 545 N/A --- 5666 5686 ug/l 100 50-150 --- --- 1.0 08/22/2018 14:25 08/24/2018 18:54 4029776 LRB Anatoxin-a 545 0.0300 --- < 0.005425 ug/l --- --- --- --- 1.0 08/22/2018 14:25 08/24/2018 18:54 4029776 LRB Cylindrospermopsin 545 0.0900 --- < 0.0 ug/l --- --- --- --- 1.0 08/22/2018 14:25 08/24/2018 18:54 4029776 FS IS-L-phenylalanine-d5 545 N/A 0787002 Central 13088 20063 ug/l 65 50-150 --- --- 1.0 08/22/2018 14:25 08/24/2018 20:51 4028692 FS IS-Uracil-d4 545 N/A 0787002 Central 4769 5686 ug/l 84 50-150 --- --- 1.0 08/22/2018 14:25 08/24/2018 20:51 4028692 FS Anatoxin-a 545 0.0300 0787002 Central < 0.0300 ug/l --- --- --- --- 1.0 08/22/2018 14:25 08/24/2018 20:51 4028692 FS Cylindrospermopsin 545 0.0900 0787002 Central < 0.0900 ug/l --- --- --- --- 1.0 08/22/2018 14:25 08/24/2018 20:51 4028692 CCM IS-L-phenylalanine-d5 545 N/A --- 11810 20063 ug/l 59 50-150 --- --- 1.0 08/21/2018 13:35 08/24/2018 21:35 4029747 CCM IS-Uracil-d4 545 N/A --- 4038 5686 ug/l 71 50-150 --- --- 1.0 08/21/2018 13:35 08/24/2018 21:35 4029747 CCM Anatoxin-a 545 0.0300 --- 0.589155 0.5 ug/l 118 70-130 --- --- 1.0 08/21/2018 13:35 08/24/2018 21:35 4029747 CCM Cylindrospermopsin 545 0.0900 --- 1.61556 1.5 ug/l 108 70-130 --- --- 1.0 08/21/2018 13:35 08/24/2018 21:35 4029747 Page 9 of 13 Page 2 of 2 EEA Run ID 247766 / EEA Report # 426737
Eurofins Eaton Analytical Run Log Run ID: 248265 Method: 546 0.00 Type Sample Id Sample Site Matrix Instrument ID Analysis Date Calibration File FS 4028693 0787002 Central DW LRB 4045942 RW LRB 4045943 RW LFB 4045946 RW LFB 4045947 RW CCL 4045950 RW QCS 4045954 RW FS 4028693 0787002 Central DW LRB 4045942 RW LRB 4045943 RW Page 10 of 13 Page 1 of 2 EEA Run ID 248265 / EEA Report # 426737
QC Summary Report Sample Type Analyte Method MRL Client ID Result Amount Target Units % Recovery RPD RPD Dil Extracted Analyzed Flag Recovery Limits Limit Factor EEA ID # FS Total Microcystins & Nodularins 546 0.300 0787002 Central < 0.300 ug/l --- --- --- --- 1.0 08/31/2018 12:38 08/31/2018 13:16 4028693 FS Percent CV 546 N/A 0787002 Central 1 % --- --- --- --- 1.0 08/31/2018 12:38 08/31/2018 13:16 4028693 LRB Total Microcystins & Nodularins 546 0.300 --- < 0.066 ug/l --- --- --- --- 1.0 08/31/2018 12:38 08/31/2018 13:16 4045942 LRB Percent CV 546 N/A --- 5.4 % --- --- --- --- 1.0 08/31/2018 12:38 08/31/2018 13:16 4045942 LRB Total Microcystins & Nodularins 546 0.300 --- < 0.0 ug/l --- --- --- --- 1.0 08/31/2018 12:38 08/31/2018 13:16 4045943 LRB Percent CV 546 N/A --- 3.799999 % --- --- --- --- 1.0 08/31/2018 12:38 08/31/2018 13:16 4045943 LFB Total Microcystins & Nodularins 546 0.300 --- 0.476999 0.5 ug/l 95 60-140 --- --- 1.0 08/31/2018 12:38 08/31/2018 13:16 4045946 LFB Total Microcystins & Nodularins 546 0.300 --- 0.418999 0.5 ug/l 84 60-140 --- --- 1.0 08/31/2018 12:38 08/31/2018 13:16 4045947 CCL Total Microcystins & Nodularins 546 0.300 --- 0.292999 0.3 ug/l 98 50-150 --- --- 1.0 --- 08/31/2018 13:16 4045950 QCS Total Microcystins & Nodularins 546 0.300 --- 0.774 0.6 ug/l 129 70-130 --- --- 1.0 --- 08/31/2018 13:16 4045954 Page 11 of 13 Page 2 of 2 EEA Run ID 248265 / EEA Report # 426737
1.00 Sample Type Key Type (Abbr.) Sample Type Type (Abbr.) Sample Type CCL Continuing Calibration Low CCM Continuing Calibration Mid FS Field Sample LFB Laboratory Fortified Blank LRB Laboratory Reagent Blank QCS Quality Control Sample Page 12 of 13
END OF REPORT Page 13 of 13
LABORATORY REPORT If you have any questions concerning this report, please do not hesitate to call us at (800) 332-4345 or (574) 233-4777. This report may not be reproduced, except in full, without written approval from EEA. Page 1 of 14
STATE CERTIFICATION LIST State Certification State Certification Alabama 40700 Missouri 880 Alaska IN00035 Montana CERT0026 Arizona AZ0432 Nebraska NE-OS-05-04 Arkansas IN00035 Nevada IN00035 California 2920 New Hampshire* 2124 Colorado IN035 New Jersey* IN598 Colorado Radiochemistry IN035 New Mexico IN00035 Connecticut PH-0132 New York* 11398 Delaware IN035 North Carolina 18700 Florida* E87775 North Dakota R-035 Georgia 929 Ohio 87775 Hawaii IN035 Oklahoma D9508 Idaho IN00035 Oregon (Primary AB)* 4074-001 Illinois* 200001 Pennsylvania* 68-00466 Illinois Microbiology 17767 Puerto Rico IN00035 Illinois Radiochemistry IN00035 Rhode Island LAO00343 Indiana Chemistry C-71-01 South Carolina 95005 Indiana Microbiology M-76-07 South Dakota IN00035 Iowa 098 Tennessee TN02973 Kansas* E-10233 Texas* T104704187-15-8 Kentucky 90056 Texas/TCEQ TX207 Louisiana* LA180008 Utah* IN00035 Maine IN00035 Vermont VT-8775 Maryland 209 Virginia* 460275 Massachusetts M-IN035 Washington C837 Michigan 9926 West Virginia 9927 C Minnesota* 018-999-338 Wisconsin 999766900 Mississippi IN035 Wyoming IN035 EPA IN00035 *NELAP/TNI Recognized Accreditation Bodies Revision date: 01/02/2018 Page 2 of 14
110 South Hill Street South Bend, IN 46617 Tel: (574) 233-4777 Fax: (574) 233-8207 1 800 332 4345 Laboratory Report Client: Hillsborough County Board of County Commissioners Report: 426759 Attn: PWS Facility ID: Sampling Point ID: Ed Watson 332 N. Falkenburd Rd. Tampa, FL 33619 Sample Event Code: EEA ID # 00001 0787001 SE8 Sample Information Priority: Status: Lab ID Code: PWS ID: Client ID Method Collected Date / Time Standard Written Final IN00035 FL6290787 Collected By: Received Date / Time 4028867 0787001 Lithia 545 08/20/18 10:45 Client 08/21/18 10:00 4028868 0787001 Lithia 546 08/20/18 10:45 Client 08/21/18 10:00 Report Summary Detailed quantitative results are presented on the following pages. The results presented relate only to the samples provided for analysis. We appreciate the opportunity to provide you with this analysis. If you have any questions concerning this report, please do not hesitate to call Jessie Brasch at (574) 233-4777. Note: This report may not be reproduced, except in full, without written approval from EEA. 09/23/2018 Authorized Signature Title Date Client Name: Report #: Hillsborough County Board of County Commissioners 426759 Page 1 of 2 Page 3 of 14
Client Name: Hillsborough County Board of County Commissioners Report #: 426759 Sampling Point ID: 0787001 PWS ID: FL6290787 Analyte ID # UCMR Result Summary Analyte Method MRL Result Units Preparation Date Analyzed Date 3311 Anatoxin-a 545 0.0300 < 0.0300 ug/l 08/23/18 13:10 08/25/18 05:23 4028867 3302 Cylindrospermopsin 545 0.0900 < 0.0900 ug/l 08/23/18 13:10 08/25/18 05:23 4028867 3301 Total Microcystins & Nodularins 546 0.300 < 0.300 ug/l 08/31/18 12:38 08/31/18 13:16 4028868 CVTM Percent CV 546 N/A 0.500 % 08/31/18 12:38 08/31/18 13:16 4028868 EEA has demonstrated it can achieve these report limits in reagent water, but can not document them in all sample matrices. EEA ID # Page 2 of 2 Page 4 of 14
Lab Definitions Continuing Calibration Check Standard (CCC) / Continuing Calibration Verification (CCV) / Initial Calibration Verification Standard (ICV) / Initial Performance Check (IPC) - is a standard containing one or more of the target analytes that is prepared from the same standards used to calibrate the instrument. This standard is used to verify the calibration curve at the beginning of each analytical sequence, and may also be analyzed throughout and at the end of the sequence. The concentration of continuing standards may be varied, when prescribed by the reference method, so that the range of the calibration curve is verified on a regular basis. CCL, CCM, and CCH are the CCC standards at low, mid, and high concentration levels, respectively. Internal Standards (IS) - are pure compounds with properties similar to the analytes of interest, which are added to field samples or extracts, calibration standards, and quality control standards at a known concentration. They are used to measure the relative responses of the analytes of interest and surrogates in the sample, calibration standard or quality control standard. Laboratory Duplicate (LD) - is a field sample aliquot taken from the same sample container in the laboratory and analyzed separately using identical procedures. Analysis of laboratory duplicates provides a measure of the precision of the laboratory procedures. Laboratory Fortified Blank (LFB) / Laboratory Control Sample (LCS) - is an aliquot of reagent water to which known concentrations of the analytes of interest are added. The LFB is analyzed exactly the same as the field samples. LFBs are used to determine whether the method is in control. FBL, FBM, and FBH are the LFB samples at low, mid, and high concentration levels, respectively. Laboratory Method Blank (LMB) / Laboratory Reagent Blank (LRB) - is a sample of reagent water included in the sample batch analyzed in the same way as the associated field samples. The LMB is used to determine if method analytes or other background contamination have been introduced during the preparation or analytical procedure. The LMB is analyzed exactly the same as the field samples. Laboratory Trip Blank (LTB) / Field Reagent Blank (FRB) - is a sample of laboratory reagent water placed in a sample container in the laboratory and treated as a field sample, including storage, preservation, and all analytical procedures. The FRB/LTB container follows the collection bottles to and from the collection site, but the FRB/LTB is not opened at any time during the trip. The FRB/LTB is primarily a travel blank used to verify that the samples were not contaminated during shipment. Matrix Spike Duplicate Sample (MSD) / Laboratory Fortified Sample Matrix Duplicate (LFSMD) - is a sample aliquot taken from the same field sample source as the Matrix Spike Sample to which known quantities of the analytes of interest are added in the laboratory. The MSD is analyzed exactly the same as the field samples. Analysis of the MSD provides a measure of the precision of the laboratory procedures in a specific matrix. SDL, SDM, and SDH / LFSMDL, LFSMDM, and LFSMDH are the MSD or LFSMD at low, mid, and high concentration levels, respectively. Matrix Spike Sample (MS) / Laboratory Fortified Sample Matrix (LFSM) - is a sample aliquot taken from field sample source to which known quantities of the analytes of interest are added in the laboratory. The MS is analyzed exactly the same as the field samples. The purpose is to demonstrate recovery of the analytes from a sample matrix to determine if the specific matrix contributes bias to the analytical results. MSL, MSM, and MSH / LFSML, LFSMM, and LFSMH are the MS or LFSM at low, mid, and high concentration levels, respectively. Quality Control Standard (QCS) / Second Source Calibration Verification (SSCV) - is a solution containing known concentrations of the analytes of interest prepared from a source different from the source of the calibration standards. The solution is obtained from a second manufacturer or lot if the lot can be demonstrated by the manufacturer as prepared independently from other lots. The QCS sample is analyzed using the same procedures as field samples. The QCS is used as a check on the calibration standards used in the method on a routine basis. Reporting Limit Check (RLC) / Initial Calibration Check Standard (ICCS) - is a procedural standard that is analyzed each day to evaluate instrument performance at or below the minimum reporting limit (MRL). Surrogate Standard (SS) / Surrogate Analyte (SUR) - is a pure compound with properties similar to the analytes of interest, which is highly unlikely to be found in any field sample, that is added to the field samples, calibration standards, blanks and quality control standards before sample preparation. The SS is used to evaluate the efficiency of the sample preparation process. Page 1 of 1 Page 5 of 14
110 South Hill Street South Bend, IN 46617 Tel: (574) 233-4777 Fax: (574) 233-8207 1 800 332 4345 Laboratory Report Client: Attn: Hillsborough County Board of County Commission Ed Watson 332 N. Falkenburd Rd. Tampa, FL 33619 Report: Priority: Status: PWS ID: 426759 Standard Written Final FL6290787 EEA ID # Sample Information Client ID Method Collected Date / Time Collected By: Received Date / Time 4028866 0787001 Lithia 544 08/20/18 10:45 Client 08/21/18 10:00 Report Summary Method 544 analysis will be performed only if the associated Screening Microcystin by EPA 546 sample result is >=0.3 ug/l. We appreciate the opportunity to provide you with this analysis. If you have any questions concerning this report, please do not hesitate to call Jessie Brasch at (574) 233-4777. Note: This report may not be reproduced, except in full, without written approval from EEA. 09/23/2018 Authorized Signature Title Date Page 1 of 1 Page 6 of 14
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Eurofins Eaton Analytical Run Log Run ID: 247768 Method: 545 0.00 Type Sample Id Sample Site Matrix Instrument ID Analysis Date Calibration File CCL 4031715 RW CY 08/25/2018 02:42 082518M545a-CY.mdb QCS 4031716 RW CY 08/25/2018 02:57 082518M545a-CY.mdb LRB 4031721 RW CY 08/25/2018 03:11 082518M545a-CY.mdb FS 4028867 0787001 Lithia DW CY 08/25/2018 05:23 082518M545a-CY.mdb CCM 4031717 RW CY 08/25/2018 05:52 082518M545a-CY.mdb Page 9 of 14 Page 1 of 2 EEA Run ID 247768 / EEA Report # 426759
QC Summary Report Sample Type Analyte Method MRL Client ID Result Amount Target Units % Recovery RPD RPD Dil Extracted Analyzed Flag Recovery Limits Limit Factor EEA ID # CCL IS-L-phenylalanine-d5 545 N/A --- 11092 12440 ug/l 89 50-150 --- --- 1.0 08/21/2018 13:35 08/25/2018 02:42 4031715 CCL IS-Uracil-d4 545 N/A --- 3450 3534 ug/l 98 50-150 --- --- 1.0 08/21/2018 13:35 08/25/2018 02:42 4031715 CCL Anatoxin-a 545 0.0300 --- 0.028178 0.03 ug/l 94 50-150 --- --- 1.0 08/21/2018 13:35 08/25/2018 02:42 4031715 CCL Cylindrospermopsin 545 0.0900 --- 0.086795 0.09 ug/l 96 50-150 --- --- 1.0 08/21/2018 13:35 08/25/2018 02:42 4031715 QCS IS-L-phenylalanine-d5 545 N/A --- 11500 12440 ug/l 92 50-150 --- --- 1.0 08/21/2018 13:35 08/25/2018 02:57 4031716 QCS IS-Uracil-d4 545 N/A --- 3991 3534 ug/l 113 50-150 --- --- 1.0 08/21/2018 13:35 08/25/2018 02:57 4031716 QCS Anatoxin-a 545 0.0300 --- 0.571185 0.5 ug/l 114 70-130 --- --- 1.0 08/21/2018 13:35 08/25/2018 02:57 4031716 QCS Cylindrospermopsin 545 0.0900 --- 1.40342 1.5 ug/l 94 70-130 --- --- 1.0 08/21/2018 13:35 08/25/2018 02:57 4031716 LRB IS-L-phenylalanine-d5 545 N/A --- 9972 12440 ug/l 80 50-150 --- --- 1.0 08/23/2018 13:10 08/25/2018 03:11 4031721 LRB IS-Uracil-d4 545 N/A --- 3185 3534 ug/l 90 50-150 --- --- 1.0 08/23/2018 13:10 08/25/2018 03:11 4031721 LRB Anatoxin-a 545 0.0300 --- < 0.0 ug/l --- --- --- --- 1.0 08/23/2018 13:10 08/25/2018 03:11 4031721 LRB Cylindrospermopsin 545 0.0900 --- < 0.0 ug/l --- --- --- --- 1.0 08/23/2018 13:10 08/25/2018 03:11 4031721 FS IS-L-phenylalanine-d5 545 N/A 0787001 Lithia 11795 12440 ug/l 95 50-150 --- --- 1.0 08/23/2018 13:10 08/25/2018 05:23 4028867 FS IS-Uracil-d4 545 N/A 0787001 Lithia 3629 3534 ug/l 103 50-150 --- --- 1.0 08/23/2018 13:10 08/25/2018 05:23 4028867 FS Anatoxin-a 545 0.0300 0787001 Lithia < 0.0300 ug/l --- --- --- --- 1.0 08/23/2018 13:10 08/25/2018 05:23 4028867 FS Cylindrospermopsin 545 0.0900 0787001 Lithia < 0.0900 ug/l --- --- --- --- 1.0 08/23/2018 13:10 08/25/2018 05:23 4028867 CCM IS-L-phenylalanine-d5 545 N/A --- 14305 12440 ug/l 115 50-150 --- --- 1.0 08/21/2018 13:35 08/25/2018 05:52 4031717 CCM IS-Uracil-d4 545 N/A --- 4643 3534 ug/l 131 50-150 --- --- 1.0 08/21/2018 13:35 08/25/2018 05:52 4031717 CCM Anatoxin-a 545 0.0300 --- 0.501312 0.5 ug/l 100 70-130 --- --- 1.0 08/21/2018 13:35 08/25/2018 05:52 4031717 CCM Cylindrospermopsin 545 0.0900 --- 1.29146 1.5 ug/l 86 70-130 --- --- 1.0 08/21/2018 13:35 08/25/2018 05:52 4031717 Page 10 of 14 Page 2 of 2 EEA Run ID 247768 / EEA Report # 426759
Eurofins Eaton Analytical Run Log Run ID: 248265 Method: 546 0.00 Type Sample Id Sample Site Matrix Instrument ID Analysis Date Calibration File FS 4028868 0787001 Lithia DW LRB 4045942 RW LRB 4045943 RW LFB 4045946 RW LFB 4045947 RW CCL 4045950 RW QCS 4045954 RW FS 4028868 0787001 Lithia DW LRB 4045942 RW LRB 4045943 RW Page 11 of 14 Page 1 of 2 EEA Run ID 248265 / EEA Report # 426759
QC Summary Report Sample Type Analyte Method MRL Client ID Result Amount Target Units % Recovery RPD RPD Dil Extracted Analyzed Flag Recovery Limits Limit Factor EEA ID # FS Total Microcystins & Nodularins 546 0.300 0787001 Lithia < 0.300 ug/l --- --- --- --- 1.0 08/31/2018 12:38 08/31/2018 13:16 4028868 FS Percent CV 546 N/A 0787001 Lithia < N/A % --- --- --- --- 1.0 08/31/2018 12:38 08/31/2018 13:16 4028868 LRB Total Microcystins & Nodularins 546 0.300 --- < 0.066 ug/l --- --- --- --- 1.0 08/31/2018 12:38 08/31/2018 13:16 4045942 LRB Percent CV 546 N/A --- 5.4 % --- --- --- --- 1.0 08/31/2018 12:38 08/31/2018 13:16 4045942 LRB Total Microcystins & Nodularins 546 0.300 --- < 0.0 ug/l --- --- --- --- 1.0 08/31/2018 12:38 08/31/2018 13:16 4045943 LRB Percent CV 546 N/A --- 3.799999 % --- --- --- --- 1.0 08/31/2018 12:38 08/31/2018 13:16 4045943 LFB Total Microcystins & Nodularins 546 0.300 --- 0.476999 0.5 ug/l 95 60-140 --- --- 1.0 08/31/2018 12:38 08/31/2018 13:16 4045946 LFB Total Microcystins & Nodularins 546 0.300 --- 0.418999 0.5 ug/l 84 60-140 --- --- 1.0 08/31/2018 12:38 08/31/2018 13:16 4045947 CCL Total Microcystins & Nodularins 546 0.300 --- 0.292999 0.3 ug/l 98 50-150 --- --- 1.0 --- 08/31/2018 13:16 4045950 QCS Total Microcystins & Nodularins 546 0.300 --- 0.774 0.6 ug/l 129 70-130 --- --- 1.0 --- 08/31/2018 13:16 4045954 Page 12 of 14 Page 2 of 2 EEA Run ID 248265 / EEA Report # 426759
1.00 Sample Type Key Type (Abbr.) Sample Type Type (Abbr.) Sample Type CCL Continuing Calibration Low CCM Continuing Calibration Mid FS Field Sample LFB Laboratory Fortified Blank LRB Laboratory Reagent Blank QCS Quality Control Sample Page 13 of 14
END OF REPORT Page 14 of 14