26 1 2002 2 JOURNAL OF FISHERIES OF CHINA Vol. 26, No. 1 Feb.,2002 :1000-0615(2002) 01-0047 - 07,, (, 200090) :,,9 12 100 120 28 30 200 ( 1 1), 90 %, 12d, 3d,, 7d, 30d,,,, 14 15d,,,20d, 0. 25mm, 0. 17mm,,0. 20mm,0. 15mm : ; ; ; ; ;; :S968. 22 : A Growth, molt, and maturation of shrimp brood stock Penaeus vannamei cultured in the river mouth area in Shanghai CAI Sheng2li, ZANG Wei2ling, DAI Xi2lin ( Fisheries College, Shanghai Fisheries University, Shanghai 200090, China) Abstract :A recirculating system was designed for shrimp brood stock Penaeus vannamei culture indoor in the low salinity area of the river mouth in Shanghai. The natural seawater with salinity of 9-12 was mixed with concentrated seawater with salinity of 100-120, which made the culture water salinity reach 28-30. About 200 shrimp brood stock (female was about 1 1) were cultured in the recirculating system for two months, and the survival rate of the shrimp was about 90 %. stimulation of the ovary maturation. Unilateral eyestalk ablation of female shrimp was done for Three days after the operation the ovary development could be observed clearly, and one week later, a part of shrimp began to spawn, but the eggs were not fertilized. One month later fertilized eggs appeared and nauplius were obtained. The results showed that shrimp brood stock P. vannamei cultured in this recirculating system could live healthily and spawn successfully. growth in shrimp brood stock P. The regularity of molting and vanamei was investigated. Before ablation of eyestalk, the mean molt interval :2001207227 :[ (00) 1-18 ] :(1957 - ),,,,, Tel : 021-65710216, E2mail :slcai @shfu. edu. cn
48 26 was about 14 15 days, and after that, the mean molt interval extends to about 20 days due to ovary development. With molting the body length of shrimp brood stock increased continuously, whether before or after ablation of eyestalk, but the mean daily increase of body length before ablation (female :0. 25mm, male : 0. 20 mm) is greater than that after ablation (female :0. 17mm, male : 0. 15). The reasons of lower growth rate and longer molting interval were discussed. Key words : Penaeus vannamei ; brood stock ; molt ; growth ; maturation ; river mouth ; water recirculating system ( Penaeus vannamei),,, [1-3 ] 1988,,,, [4 ], ( P. ( P. japonicus) ( P. monodon) chinensis) ( Macrobrachium rosenbergii),,,,,,,,,,, [5,6 ] [7 ],,,,,,,,,,, [8 ],,, (1),,,, ; (2), 1 1. 1 2001 3 20 205, 105, 12. 80 0. 43 cm, 100, 12. 60 0. 35cm 4, 10,,, Q 1. 2 ( ) 3 20, 12d, 27 28, 27 28, 400 500Lx,p H 8. 1 8. 5 4 2,, 5 17
1 : 49 58d ( Nereis spp. ) ( ), ( Smonovacula spp. ), 4 1,7 :30 17 :30, 5 % 10 % 1. 3,, (9 12),,, 27 28, 50 %, ( 1) 1 Fig. 1 Sketch of the recirculating system A :, 17. 32m 2, 1m B : B 1, (100 ) B 2 10 C : C 1 :, C 2 :, C 3 :,C 4 : D :, 1 000W ( WaterTch,:UV12AS) B 2 C 4 1,2t h - 1 8-10h 2t h - 1, 6 8h 1 DP : ECB : WDM : A :Hatching tank, ellipse, depth : 1m, area :17. 32. B : Egg2collecting case. A egg2collecting bag is set in B 1. 10 layer of polythene nets are set in B 2. C : Biological filter. C 1 : multi2zeolites, C 2 :plastic balls, C 3 : zeolites, C 4 :polythene net. D : Germicide light. UV2light, 1 000W ( WaterTec made in USA, Model :UV12AS). A submersible pump with a 2t h - 1 of flow rate is set in B 2 and B 4. The UV light is on for 8-10h day - 1, and the total water can be circulated once in 6-8h. DP :diving pump ECB : egg collecting bag WDM : water draining mouth 1. 4 48h,, ( ) 28 30, (10 15) 10-6 g ml - 1, 24h, NaS 2 O 3,,, (10 15) 10-6 g ml - 1 EDTA2Na 8 :00 p H COD 60cm,,,,, 20cm, 12 12h,,400 500lx,,,, 1,,,, 17 :00-22 :00, 2 2. 1,, 4 2,0. 82 5. 15mg L - 1,, 1mg L - 1, (3 21 4 6 ),,0. 01 0. 4mg L - 1,2mg L - 1, 4mg L - 1,
50 26,1 2mg L - 1,,, 20cm NH 3 - N NO - 2 - N,, 3 20 12h, 5 17, ( 1), :95. 1 % 98. 5 % 94. 3 %,, Tab. 1 Survive rate of P. 1 vannamei during experimental period ( - ) date (M - D) 03-20 before transportation 03-20 after transportation 3-21 04-01 after temporary culture 04-02 05-17 after spawning ( ) number of shrimp ( ) number of dead ( %) survive rate 205 195 192 181 10 3 11 95. 1 98. 5 94. 3 2. 2 2001 3 20 5 17, 4, 2 (58d),,1. 07cm, 0. 90cm ( P < 0. 01), (12d) 12d ( P > 0. 05) ( 2),, (0. 25mm) 1 (0. 21mm), 5 5 5 17,, 0. 08mm,, 2 1 Fig. 2 Growth of P. vannmei during experiment period 0. 20mm 0. 14mm,, 0. 18mm ( 2),, 2. 3 3 20 4 2 ( ), 3 p H,, 5 %,,,, 5 %, 192, 162,13. 5, 7. 03 %, 10. 25 % 12d,84 %
1 : 51 2 Tab. 2 Growth of P. vannmei during experimental period ( - ) date (M - D) 3-20 4-2 5-5 5-17 female male / cm body length / mm increase per day / cm body length / mm increase per day 12. 80 0. 43 (a) 13. 10 0. 51 (a) 13. 78 0. 38 (b) 13. 87 0. 37 (b) 3 0. 25 0. 21 0. 08 12. 60 0. 35 (a) 12. 84 0. 21 (a) 13. 29 0. 36 (b) 13. 50 0. 39 (b) 0. 20 0. 14 0. 18 3 : ( P > 0. 05), ( P < 0. 01), ( F = 15. 11, F = 9. 55 ) Notes : The different letters in the same line show significant difference ( P < 0. 01), and the same letters show insignificant different ( P > 0. 05), ( F female = 15. 11, F male = 9. 55), 7 %, 2 3d,,, 14 15d 4 2 5 17 4 44d, 440,, 10, 5. 38 % 2. 3, 3 19 20d, Fig. 3 Molting of P. vannmei before eyestalk ablation 5 6d 1 16d (04-03 04-19),, 2 20d (04-19 05-9),, ( ), 4 ( ) Fig. 4 Molting of P. vannmei after eyestalk ablation
52 26 2. 4 3d,6 7d, 30d,, 457, 137, 33 % ( 3),87. 5 % 3 Tab. 3 Spa wn, fertilization, and hatch rate of P. vannamei ( - ) date (M - D) 04-10 05-05 before fertilizing 05-05 05-17 after fertilizing 10 4 number of eggs 319. 7 10 4 number of fertilized eggs ( %) fertilizing rate 10 4 nauplius ( %) hatching rate 137 61 44. 5 20. 1 33. 0 3 3 : 3 :the hatching rate of the fertilized eggs. 3 3. 1,,,28 35, 9 12,,, (28 ),,,,,,1m 3. 2, (1) NH 3 2N NO 2 2N :,,, NH 3 2N NO 2 2N,,,,, (2) :, 28,,,, (3) :,, (1 1), ( 10 ), (4) :,,,,, 90 % (5) :,,, 3. 3,,
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