2013 26 2 227 233 Forest Research 1001-1498 2013 02-0227-07 * 550025 N P K N P 1 N P 56. 65% 44. 32% 1 K 221. 99% 200. 00% N K P N K 1 N P K S791. 248 A Influence of Ectomycorrhiza on Nutrient Absorption of Pinus massoniana Seedlings Under Water Stress WANG Yi DING Gui-jie Institute of Forestation and Ecology Guizhou University Guiyang 550025 Guizhou China Abstract The effect of ectomycorrhiza on nutrient absorption of potted seedlings of Pinus massoniana under water stress was studied in greenhouse by inoculating Pisolithus tinctorius Cenococcum geophilum Cantharellus cibarius Fr. Suillus luteus L. Fr. Gray respectively. The results showed that ectomycorrhiza could significantly improve the absorption of content of N P and K in drought stress. The contents of N P and phosphatase activities of mycorrhizal seedling increased at first and then decreased with the water stress increase and reached the maximum in moderate stress. The seedling of inoculation Suillus luteus1 had the best absorption to N P and increased 56. 65% and 44. 32% respectively compared to control group. The content of K in mycorrhizal seedling inoculating Pisolithus tinctorius and Suillus luteus1 increased at first and then decreased with the increase of water stress and reached the maximum in the light stress. They increased by 222% and 119% respectively compared to the control group. N and K mainly distributed in the leaf while P equally distributed in root stem and leaf. The formation of mycorrhizal benefited the transportation of N and K upward. Inoculating Suillus luteus1 in mild and moderate stress had the best comprehensive effect in absorption and content of N P and K and at the same time it could promote seedling growth and enhance seedling drought resistance. Key words Pinus massoniana water stress ectomycorrhiza seedling nutrient 2012-02-05 863 2011AA10020301 2009 9 2011 2009002 1985.. *.
228 26 Pers. Coker et Couch C Cenococcum geophilum Fr. T 1 4 CK 2-5 Gamalero 6 AMF 1. 2 5 MMN 7 28 10 d PD Azcón 8 500 ml AMF N 300 ml Siddiqui 9 1 cm 5 25 20 d 16-17 10-15 1. 3 Pinus massoniana Lamb. 75% 1 min 4 40 24 h 25 9 2 0. 14 Mpa 124 126 2 h 250 mm 280 mm95% 1 1. 1 2010 1 2011 10 800 ml m - 2 Sp7 S7 CK Sp1 S1 Suillus luteus L. Fr. Gray Cantharellus cibarius Fr. G Pisolithus tinctorius 5 kg - 1 1 1. 4 30 cm 1 / g kg - 1 N P K / mg kg - 1 N P K / mg g - 1 Fe Mn Cu Zn / g kg - 1 Ca Mg / g kg - 1 0. 16 0. 36 14. 96 66 11 164 16. 3 8. 2 1. 6 5. 6 2. 9 0. 4 5. 88 1. 5 A 25 ± 5 % 6 CK B S7 G S1 C T 5 % 50 ± 5 % 35 ± 5 % 3 15 2 4 1 65 ±
2 229 S7 G S1 T N CK 48. 98% 60 d 41. 59% 56. 65% 26. 75% CK N 1. 6 200 20 N N 100% 0 N 18 CK 2 C N 11. 8 g N P kg - 1 1 S7 S1 K N 19 18. 9% 3. 07% S7 S1 1. 7 N EXCEL SPSS18. 0 24. 9% 20. 4% N T 2 2. 1 N S1 N 2 p < 0. 01 11. 76% S7 S1 N S7 2 N P K g kg - 1 N S7 8. 79 ± 0. 19 cc 10. 46 ± 0. 12 fe 10. 98 ± 0. 21 ed 5. 66 ± 0. 87 bb G 9. 37 ± 0. 03 dd 7. 11 ± 0. 02 bb 10. 44 ± 0. 03 cc 6. 37 ± 0. 11 cc S1 9. 59 ± 0. 03 ee 9. 88 ± 0. 01 ed 11. 55 ± 0. 12 fe 6. 37 ± 0. 01 cc C 11. 80 ± 0. 06 ff 7. 84 ± 0. 02 cc 10. 80 ± 0. 10 dd 6. 99 ± 0. 20 dd T 8. 10 ± 0. 01 bb 8. 04 ± 0. 03 dc 9. 34 ± 0. 07 bb 7. 15 ± 0. 07 ed CK 7. 81 ± 0. 01 aa 5. 85 ± 0. 01 aa 7. 37 ± 0. 02 aa 2. 72 ± 0. 04 aa P S7 0. 44 ± 0. 02 bb 0. 53 ± 0. 03 db 0. 79 ± 0. 12 bb 0. 45 ± 0. 03 bbc G 0. 46 ± 0. 01 bcb 0. 48 ± 0. 06 cb 0. 78 ± 0. 08 bb 0. 47 ± 0. 05 bbc S1 0. 50 ± 0. 03 cdbc 0. 62 ± 0. 02 ec 0. 76 ± 0. 11 bb 0. 51 ± 0. 11 bc C 0. 53 ± 0. 10 dec 0. 71 ± 0. 10 fd 0. 72 ± 0. 04 bb 0. 40 ± 0. 08 aab T 0. 56 ± 0. 02 ec 0. 41 ± 0. 02 ba 0. 68 ± 0. 06 bab 0. 48 ± 0. 04 bc CK 0. 38 ± 0. 01 aa 0. 35 ± 0. 01 aa 0. 56 ± 0. 02 aa 0. 37 ± 0. 03 aa K S7 4. 60 ± 0. 30 ee 1. 95 ± 0. 10 bb 1. 93 ± 0. 08 bb 1. 99 ± 0. 06 cc G 4. 10 ± 0. 12 dd 3. 08 ± 0. 05 dd 1. 56 ± 0. 05 aa 1. 71 ± 0. 10 bb S1 3. 76 ± 0. 22 cc 4. 23 ± 0. 14 ee 3. 44 ± 1. 04 ee 2. 32 ± 0. 23 dd C 3. 86 ± 0. 14 ccd 4. 54 ± 0. 20 ff 2. 89 ± 1. 10 dd 2. 70 ± 0. 17 ee T 3. 12 ± 0. 06 bb 2. 64 ± 0. 11 cc 2. 52 ± 0. 08 cc 2. 69 ± 0. 04 ee CK 1. 76 ± 0. 02 aa 1. 41 ± 0. 09 aa 1. 55 ± 0. 06 aa 1. 41 ± 0. 03 aa p < 0. 05 p < 0. 01
230 26 1 N 2. 2 P P P P P P 2 CK P P P p < 0. 05 P S1 44. 32% > C 42. 09% > S7 34. 39% > G 31. 97% > T 28. 66% P P CK 2 T P 0. 56 g kg - 1 2 C P 33. 0% P p < 0. 05 S7 78. 8% > G 70. 2% > S1 51. 3% > C 34. 7% > T 22. 8% S7 G S1 P CK p < 0. 013 P 2 P 3 mg g - 1 S7 57. 02 ± 1. 12 de 55. 92 ± 4. 32 ef 55. 22 ± 3. 56 cd 34. 34 ± 2. 13 bb G 50. 05 ± 2. 23 cc 50. 68 ± 2. 56 cc 53. 81 ± 2. 45 cc 54. 23 ± 3. 23 dd S1 18. 38 ± 2. 15 bb 21. 29 ± 3. 24 bb 70. 32 ± 4. 34 ee 69. 38 ± 5. 52 fe C 65. 00 ± 3. 43 ef 53. 38 ± 2. 43 de 67. 02 ± 2. 30 df 67. 32 ± 3. 65 ef T 51. 32 ± 2. 35 cd 52. 32 ± 1. 32 cdd 51. 96 ± 2. 26 bb 51. 57 ± 4. 23 cc CK 14. 76 ± 3. 53 aa 14. 24 ± 4. 45 aa 20. 94 ± 4. 54 aa 13. 76 ± 2. 51 aa S7 6. 58 ± 0. 12 ee 3. 80 ± 0. 23 dd 5. 57 ± 0. 32 ee 5. 32 ± 0. 12 dc G 3. 80 ± 0. 23 dd 4. 05 ± 0. 13 ee 4. 56 ± 0. 23 dd 1. 52 ± 0. 10 ba S1 6. 59 ± 0. 25 ee 4. 56 ± 0. 14 ff 10. 89 ± 0. 41 ff 9. 88 ± 0. 13 ed C 2. 03 ± 0. 31 cc 3. 29 ± 0. 22 cc 3. 80 ± 0. 25 cc 1. 35 ± 0. 23 aba T 1. 52 ± 0. 24 bb 2. 53 ± 0. 24 bb 3. 29 ± 0. 34 bb 3. 29 ± 0. 21 cb CK 1. 21 ± 0. 42 aa 1. 15 ± 0. 31 aa 1. 50 ± 0. 56 aa 1. 33 ± 0. 10 aa 2. 3 K CK 2 p < 0. 01 K K S7 G T K
2 231 S1 C K C S1 K K 3 C S1 4 K p < 0. 01 17. 7% 12. 6% N P K K 4 MS F P N P MS F P K MS F P A 3. 13 511. 50 0. 000 0. 06 43. 30 0. 000 6. 10 1. 12 0. 000 B 45. 80 7. 49 0. 000 0. 27 208. 90 0. 000 7. 48 1. 37 0. 000 A B 5. 26 858. 90 0. 000 0. 02 12. 20 0. 000 1. 34 245. 40 0. 000 0. 006 0. 001 0. 005 p < 0. 01 3 K 2. 4 N P K 4 N S1 N N N P S7 G S1 P p > 0. 05 4 N P K K K K K S1 K K
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