Eucryotic Cells: Power Plants for ExoPolySaccharides EPS: carbohydrate-unimers oligomers polymers nano-materials
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1 Eucyotic Cells: Powe Plants fo ExoPolySacchaides EPS: cabohydate-unimes oligomes polymes nano-mateials selection / beeding vaieties of mico-oganisms / GMO Cabon-feedstock + conditions of micobial gowth puification / factionation of poduced EPS age of the femente metabolic activity of mitochondia to poduce enegy (AP) fo the biosynthesis of EPS
2 Mico Powe Plants Geen Chemisty Xanthan - Poduction by Xanthomonas campestis A.Palaniaja, V.Jayaaman, Poduction, ecovey and applications of xanthan gum by Xanthomonas campestis, Jounal of Food Engineeing,106,1,1-12, 2011 J.K.Leela, G.Shama, Studies on xanthan poduction fom Xanthomonas compestis, Biopocess Engineeing, 23, , 2000 gam-negative bacteium penta-sacchaide-polyme Xanthan batches: micobial vaiety, gowth conditions, puification / factionation, age of the femente
3 Xanthan Matin Chaplin, London South Bank Univesity (1 4) D-glucose backbone + (1 3) linked tiose-banches (60-70%) C6-acetylated mannose, (1 2) glucuonic acid, (1 4) (30-40%) C4-pyuvate mannose backbone backbone + banches aqueous media att + ep foces xanthan / wate / modifie (XWM) systems
4 Molecule Polyme Chaacteistics Hydocolloidal Chaacteistics of PS/W/M-systems W C1 C2 atoms / molecules polyme / polyme atoms / molecules inteface nano-stuctued objects nano-stuctued object / inteface W C 3 C L W C: electon chage C p W A R1 R2 A R 3 W W 6D 6D ( R1 R2) 6D A: Hamake constant (inteface Fee Enegy) dimension / mobility - affecting envionment (volume) - stability
5 Aggegates Hydocolloids: Inteface / Volume W C 6 W A R 6D
6 PS/W/M: ecovey / concentation / medium Xanthan: young femente Homogeneous XWM-patition: aqueous 0.05 M ZnCl 2 / MgCl 2 / CaCl 2 initial 3.0 mg/ml ecovey: 31% initial 3.0 mg/ml ecovey: 70% initial 3.0 mg/ml ecovey: 41% 0.05 M Mn Mw Mz Mw/Mn ZnCl MgCl CaCl
7 Distibutions in homogeneous Systems: Dimensions without Refeence Mateials (absolute): SEC + Visc appaent Mola Mass: mass / LS K c 1 1 = + 2 A2 c R P Mw intecept: 1 / M w weight aveage mola mass(app) [g/mol] sepaation distibutions SEC: V e [nm 3 ] distibution detection mean values mass: mass faction (ecovey) (light) scatteing: M (mola mass) - appaent viscosity: V e excluded volume, confomation slope (q 2 0): R g adius of gyation [nm] slope (c 0): A 2 inteaction potential [ml mol g -2 ]
8 Distibutions in homogeneous Systems: Excluded Volume (V e D) fom SEC + Intinsic Viscosity [ƞ] V 1 R 3 3 e M e V 2.5 N 4 A e Phamacosmos Dextan Standads Dextan 25 (Mp g/mol; [η] 16.9 ml/g) Dextan 50 (Mp g/mol; [η] 23.0 ml/g) Dextan 80 (Mp g/mol; [η] 31.5 ml/g) Dextan 150 (Mp g/mol; [η] 39.4 ml/g) Dextan 270 (Mp g/mol; [η] 49.4 ml/g) Dextan 410 (Mp g/mol; [η] 55.4 ml/g) Dextan 670 (Mp g/mol; [η] 70.8 ml/g)
9 mass faction (aea=1.0) mass faction (aea = 1.0) Xanthan/W/M: modifie-conc (CaCl 2 ) 8.00E E-03 Xanthan 'low viscosity' XWM system Homogeneous patition: aqueous 0.1, 0.5, 1.0, 2.0 M CaCl 2 65 magnetic stiing 6.00E E E E E E E E Re [nm] CaCl₂ Mw Mn Mz Mw/Mn Mz/Mw 0.1 M M M M lg (M) [lg (g/mol)]
10 Distibutions in heteogeneous systems: Dim without Refeence Mateials: AF4 - mass / LS AF4: asymmetic flow Field Flow Factionation sepaating dissolved + dispesed objects in continuous phase due to diffeences in diffusive mobility D t F w 6 V 0 2 c 0 1 t assuming spheical geomety R H kb 6 D D : diffusive coefficient, t 0 : void time, F c : coss-flow, w: AF4-channel thickness, V 0 : void volume, t : etention time, : tempeatue, k B : Boltzmann constant, η: viscosity
11 Hydocolloidal Systems dissolved polymes + aggegates + objects D t F w 6 V 0 2 c 0 1 t R H kb 6 D conventional pepaation of XWM-solution / dispesion by 20 h stiing at 60 C with - SEC-analysis of the homogeneous patition - AF4-analysis of the mix of homogeneous + heteogeneous patition XWM-solution / dispesion pepaed by ultasonic-finge suppot at 25 C.
What molecular weight polymer is necessary to provide steric stabilization? = [1]
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