THE BIG PICTURE. Aim: Study the electrical properties of biomolecules suspended in solution on application of electric field
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1 Effect of Conductivity and Viscosity in the Velocity Characteristics of a fluid flow induced by nonuniform AC electric field in electrolytes on microelectrodes Pritesh Parikh, Astha Sethi, Samatha Benedict, Subimal Deb and Souri Banerjee Department of Physics, Birla Institute of Technology and Science- Pilani, Hyderabad Campus,India
2 THE BIG PICTURE Aim: Study the electrical properties of biomolecules suspended in solution on application of electric field ACEO Trapping of Molecules DEP ACEO and DEP: opposite in nature frequency dependent.
3 ELECTRO-OSMOTIC FLOW Caused by Coulomb force on net mobile charge In the process, it creates an electric double layer Fluid is pushed away from the gap between the electrodes towards the electrode surfaces [1] Green et al, Physical Review E,2002
4 NUMERICAL MODEL Coplanar parallel electrode (CPE) geometry was used and the phenomena of AC electro-osmosis was simulated using COMSOL Multiphysics Software. Symmetry line Gap between Electrodes : 25 m Electrolyte: KCl = 2.1x10-3 S/m = Pa. s Double Layer ~ 25 nm
5 SIMULATION DETAILS Frequency of the applied electric potential : 1Hz to 80kHz Electric field was computed using Electric currents interface Fluid Flow was computed using the Creeping flow interface which makes use of the time dependent Navier Stokes equation The Debye layer was modeled as a capacitive boundary condition on the electrode surface
6 SIMULATION DETAILS The electro osmotic velocity was computed using the inbuilt equation(helmholtz Smoluchowski formula) System response studied for : 300 sinusoidal cycles Symmetry of electrodes: Only the right half electrode geometry simulated The time averaged velocity was computed at various distances along the electrode surface
7 Frequency 500Hz ELECTRIC POTENTIAL
8 FLUID FLOW PATTERN
9 Velocity(m/s) VELOCITY MAGNITUDE AT DIFFERENT DISTANCES ON ELECTRODE SURFACE 4.9E E E-04 d=13 d=17 3.4E-04 d=21 d=25 2.9E E-04 d=29 1.9E E E E E Frequency(Hz)
10 FLUID FLOW AT DIFFERENT FREQUENCIES Frequency 1000Hz
11 FLUID FLOW AT DIFFERENT FREQUENCIES Frequency 10000Hz
12 FLUID FLOW AT DIFFERENT FREQUENCIES Frequency 30000Hz Reversal of flow at high frequency
13 Velocity(m/s) OBSERVATIONS FROM PLOT 4.9E E E-04 The velocity maximum shift to lower frequencies for larger distances d=13 3.4E-04 d=17 2.9E-04 d=21 2.4E-04 d=25 1.9E-04 d=29 1.4E E E E Frequency(Hz) d: Distance from the edge of electrode
14 Velocity(m/s) SECOND PEAK 4.5E E E E E E E E E-06 d=13 d=17 d=21 d=25 d=29 0.0E Frequency(Hz)
15 Velocity(m/s) OBSERVATION FROM PLOT Another peak smaller by one order of magnitude was observed at higher 4.5E-05 frequencies 4.0E E E E E E E E-06 d=13 d=17 d=21 d=25 d=29 0.0E Frequency(Hz) The velocity max shift to higher frequencies for larger distances
16 Velocity(m/s) VARIATION IN CONDUCTIVITY 5.0E E E E E E E-04 sigma= sigma=.0021 sigma= E E E E Frequency(Hz)
17 Velocity(m/s) OBSERVATION FROM PLOT 5.0E E-04 Shift in peak velocity to Higher frequencies at Larger conductivity values 4.0E E E E E-04 sigma= sigma=.0021 sigma= E E E E Frequency(Hz)
18 Velocity(m/s) VARIATION IN VISCOSITY 1.8E E E E E E E-04 eta= eta=.001 eta= E E E Frequency(Hz)
19 Velocity(m/s) OBSERVATION FROM PLOT 1.8E-03 Shift in peak velocity Magnitude to lower values at higher viscosity values 1.6E E E E E E-04 eta= eta=.001 eta= E E E Frequency(Hz)
20 SUMMARY Observation of a weak second maxima in Velocity versus Frequency plots indicative of complete reversal of fluid flow Examination of the distance dependence of the two peaks. Observation of effect of conductivity and viscosity on electro osmotic velocity as a function of frequency
21 CONCLUSION The conductivity, viscosity of the electroylte and frequency of applied electric field affect the electro osmotic velocity. The direction of flow can be tuned by the frequency of applied electric field The magnitude of the peak velocity can be tuned by the viscosity The position of the peak velocity as a function of frequency can be tuned by the conductivity
22 THANK YOU
23
24 BOUNDARY CONDITIONS : Potential u: Normal velocity v: Tangential vel u ACE :Electro-osmotic vel
25 COMPARISION [1] Green et al, Physical Review E,2002
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