Pesticide Use Permit Application

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1 inistry of nvironment esticide se ermit pplication his application is submitted under the ntegrated est anagement ct to obtain a permit for the use of pesticides as prescribed under the ntegrated est anagement egulation ()ection 18. Following the submission of this application, the applicant will be provided an application number by ministry staff and instructed to consult the public in accordance with ection 60. he applicant must submit evidence of consultation and a statement of action proposed in response. esticide use may not proceed unless a permit has been issued. fee of $1,000 must be submitted with this application. he fee is non-refundable once processing has occurred.. F Q. 1. Full egal ame of pplicant his name must be a imited or ncorporated ompany registered with the B egistrar of ompanies. lease provide the name exactly as registered along with the incorporation registration number. f the applicant is not a imited or ncorporated ompany use full personal name. ermaq anada td B pplicant s ame B.. ncorporation egistration # om Foulds Business perating ame, if different from above (i.e. oing Business s ) ame of the ontact erson for the ermit sland ighway ampbell iver B 9 22 uite # treet ame and umber ity rovince ostal ode tom.foulds@cermaq.com (250) ext mail ddress rea ode + elephone # rea ode + ell hone # 2. ame of the wner or anager of the reatment ocation reatment ocations are rown and operated by ermaq anada under icence of ccupations: and File # s , & urpose of roposed esticide se opical removal of ea ice (lepeoptheirus salmonis) ermit pplication umber: 4. roposed tart and nd ate of esticide se ommencement ate r. o. ay ompletion ate r. o. ay 5. escription of the Geographic rea to be covered by the ermit escribe the location of the proposed treatment area: Finfish quaculture facilities are located on the ast oast of ancouver sland in kisollo hannel and aza assage in the iscovery slands, approximately 32km orth of ampbell iver. ee attached aps x 4.(General rea, Brent sland, enture oint & aza sland Facility aps) For ffice se nly ate received: ate permit mailed: ayment ( ) ot ttached ( ) ncomplete ( ) or ncorrect ( ) og o.: ermit xpiry ate: ermit o: pplication rocessed by: eferral to B ( ) egions ( ) ( ) ( ) egion(s):

2 6. ype of esticide se equiring a ermit lease check the category below that corresponds with your intended pesticide use. ote: the category must be for a pesticide or pesticide use as prescribed under the ntegrated est anagement egulation ection 18. a) ermit-restricted pesticide b) erial application of a pesticide c) se of a pesticide in or on a body of water d) se of a pesticide on public land not prescribed as requiring a licence or confirmation e) se of a pesticide under a confirmation or licence requiring an exemption from the applicable prescribed standards in ivision 7, art 2 of f the prescribed use for which a permit is required falls under e) above, provide details of the regulated standard that the permit applicant considers impractical to meet and why. ropose alternative terms and conditions that in the opinion of the applicant will not cause unreasonable adverse effects. 7. esticide nformation a. ctive ngredient (common name) ydrogen eroxide ( 2 2 ) b. esticide rade ame c.... o. d. pplication ate e. reatment rea f. Quantity (kg a.i./ha) (ha) (kg a.i.) nterox aramove g/t 3.44a 615,600t 8. pplication ethod aramove 50 will be used in accordance with the directions on the product label and the. pplication will be administered by either enclosed tarpaulin or ell Boat. arpaulin treatments will require that the fish are seined and placed into tarpaulins to confine them in a specific volume of water, where the aramove can be added quickly to attain the prescribed treatment concentrations. he fish will be held in the tarpaulins for a maximum of 30 minutes with ongoing oxygen monitoring. pon completion of the timed treatments the tarpaulins will be released allowing in saltwater which will then neutralize the bath. ell boat treatments will require that the fish are seined and pumped into the well boat and held in the required concentration of aramove for 30 minutes. he fish will then be pumped out of the well boat and back into the net cage. he treatment bath inside the boat will then be neutralized with additional seawater, filtered and discharged. he health and welfare of the fish stocks will be monitored throughout the treatment process by Fish health staff. 9. ignature he undersigned declares that the use of pesticides under the authority of this permit will comply with the ntegrated est anagement ct and egulation. rint ame: om Foulds osition itle: icence and ompliance fficer ignature: ate: 12 th eptember, 2017

3 i t k a G a k e J a p e n o x t l i n a k e G B F u l s e q u a h G ) e s l i n a k e G G B a p e t J a m e s G e l e g r a p h r e e k Z B e a s e a k e B o w s e r Z B a s s i a r G ( ) o w e r o s t G B u t e d a l e a p e c o Q Z a p e o B e l a B e a p e e m a n o F a u t i o l i s o n a r b o u r o r i c e a k e a m u o t Z B B c a k e h i t e s a i u t s u k o e B w i k e n o l e r t B a y i m p k i s h a k e a k e B a b i n e G a k e a k l a a k e G e r m a n s e n a n d i n g r e m b l e u r B F r a n c o i s, a k e o t s a a k e Z B e t a c h u c k e l s e y B a y F l o r F F h a r B c h e n t l o G r e a t e n t r a l F r a s e r a k e B a m f i e l d a p e F l a t e r y a k e a t X e z e r o n F J o w e l a k e h i l k F a z k o J a s e k o a k e s G B Z X G G o t c h o a k e Q B G n d e r B Q G B i B i l o e t t a v e a k e B G e l l s s e f l y i k e l y 1 00 B F a r i s o n a k e B r e e n s e f l y F G c B t e G a k e B G e f l e y B l u e i v e r B a k e i n b a s k e t o l d s t r e a m k a n a g a n a k e B a k e a b e l a k e G u m b y p p e r o w e r r o w a k e a k e G a k e G u n c a n a k e B learly mark the location of sites where pesticide use is intended G F B F B e n n e t t l i n a g i s h F 104 B F ( G 10 4 J J e a c e F 104 G G F 6 k e e n a a t l a t u i h u t a d e G F ( ) F F 10 4 F F B B F J F X J ' B 7 m i n e c a i n c h i t u a r t J c G ( ) F Q G a h t s a Q u e s n e l o b s o n a k e a o r l n a h i m a k e o r 09 3 l e a r w a t e r l a B 093 d a k e a n l o t e s l 5 g B n l a i a h o o d a n i m a r i b o o Q G l a y o k o B G o B o n a p a r a k e a r i e r e B n B F Q G G B F l F 09 3 G B B u r t l e d a m s a k e h u s w a p G a m l o o p s r o w t t s o n B J B o u t h e r n y t t o n k d g e w o o d 1 o w e r y u q u n t e r i o r Q G X G a n c o u v e r a i n l a n d 08 2 B t t F 09 2 G 09 2 F G B G e F s l a n d B G ( ) B o r t e n f r e w J F B B B l o c a n G B B 08 a k 2 e 4 o o t e n a y o o t e n a y F B ( ) J F F 082 G ( ) a k e o c a n u s a ocation of treatments

4 odales h ermaq anada td iscovery slands perations onora sland 1:300,000 enture t. [ kisollo h. [ aza sl. [ Brent sl. iscovery hannel Quadra sland a n c o u v e r s l a n d ortez sla egend [ arine Facilities " ermaq ffices ilometers arehouse/urchasing " " ermaq Q trait of Georgia ² ate aved: 10/07/2017 1:56:52

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8 ermaq anada td eptember 2017 ppendix : ea ice verview ea lice are parasitic copepods (rder iphonostomatoida, Family aligidae) found in the marine environment. he two genera, epeophtheirus and aligus, are commonly found parasites of wild and farmed salmonids. he dominant species affecting salmonids in British olumbia waters is epeophtheirus salmonis. he life cycle (Figure 1) involves 2 larval stages, planktonic nauplii and the infectious phase copepodid which first attaches to the host. he copepodid enters the chalimus stage denoted by a specialized frontal filament that is used to attach to the host. pon reaching the pre-adult stage the louse becomes motile before finally maturing into reproductive adults. he generation time is temperature dependant, and can vary from 38 to 72 days. ice are readily transferred from wild adult returning salmon in late summer and early fall, via egg laying adult female lice dispersing nauplii into the water column. ice levels, survival and fecundity are influenced by a number of factors including adult wild returns (more adult wild returning salmon = more lice), salinity (lower salinity = fewer sea lice as they do not tolerate lower salinities), temperature (higher temperature = faster lice growth and reduced life cycles), oceanographic factors impacting distribution, and presence of filter feeders such as mussels that feed on nauplii and copepodids within the planktonic community. o reduce the potential for lice from farmed fish to impact outmigrating smolts, lice levels are monitored every two weeks during the smolt outmigration period (arch 1 st to June 30 th ). uring this period treatment or harvest to reduce lice levels is required if levels rise above 3.0 motile lice (pre-adults and adults) per fish sampled. ice levels routinely increase late summer and early fall with returning adult pacific salmon. ea lice use specialized mouthparts to graze upon salmon, removing skin, mucus, and tissue. mpacts of grazing include epithelium loss, bleeding, and tissue necrosis. his can affect the fish s ability to prevent physical damage and to fight off bacterial infections. ther impacts can include reduction in appetite, feed conversion rate and therefore growth. hen levels reach an average of 3 motile sea lice per fish a treatment using the active ingredient mamectin benzoate () has historically been used effectively. o measure the effectiveness of the drug on the sea lice, a bioassay is performed. his assay consists of sampling sea lice from the infected population to be treated then exposing the sampled louse to increasing concentrations of the active ingredient. s indicated from the results found in table 1 we have observed that lice sampled from Bawden Farm have remained sensitive to mamectin benzoate. owever in table 2, a subsequent bioassay showed resistance to mamectin benzoate. t is the genetic variation within different lice populations that can, if repeatedly exposed to the same product, result in reduced sensitivity to products. ny effective integrated pest management plan, including all farmed animals and crops, requires coordinated treatments, coordinated fallowing, and alternation of treatment classes. he coordination of treatments and fallowing of sites is already put in place. n alternative product such as ydrogen eroxide would be a useful tool to ensure we responsibly manage pests such as sea lice. ydrogen eroxide ( 2 2 ) pplication for the treatment of ea ice

9 ermaq anada td eptember 2017 nfective tage va containing eggs trailing the adult female release nauplii into the water column Figure 1: lanktonic auplius 1 & 2 transform in 4-5 days to the infective stage opepodid. nce attached, becomes halimus stages to pre-adult to reproductive adults in days, depending on temperature and feeding. ydrogen eroxide ( 2 2 ) pplication for the treatment of ea ice

10 ermaq anada td eptember 2017 able 1 Bioassay results for ea ice sampled from Bawden oint 07-Jun-17 exposed to increasing concentrations of mamectin benzoate () for 0 hours and 24 hours. 0 hr (# of live lice) eplicate oncentration (ppb) Female #1 Female #2 Female #3 ale #1 ale #2 ale # hr (# live lice) eplicate oncentration (ppb) Female #1 Female #2 Female #3 ale #1 ale #2 ale # Female ethal ose (50%) = ppb ale ethal ose (50%) = ppb pper 95% = 105 ppb ower 95%= 65.9 ppb pper 95%= ppb ower 95%= 173 ppb ydrogen eroxide ( 2 2 ) pplication for the treatment of ea ice

11 ermaq anada td eptember 2017 able 2 Bioassay results for ea ice ampled from a site exposed to increasing concentrations of namectin benzoate for 0 hours and 24 hours 0 hr (# of live lice) eplicate oncentration (ppb) Female #1 Female #2 Female #3 ale #1 ale #2 ale # hr (# of live lice) eplicate oncentration (ppb) Female #1 Female #2 Female #3 ale #1 ale #2 ale # ydrogen eroxide ( 2 2 ) pplication for the treatment of ea ice

12 ermaq anada td eptember 2017 torage and pplication ppendix aramove 50 will be stored in a fenced secure location prior to application at farms. his requires isolated storage and separation, free from combustibles and other chemicals/flammable materials. he storage tanks will regularly be inspected and monitored by cameras. afe and secure storage and transport in appropriate containers will ensure the containment of aramove 50 to prevent adverse impacts to water quality and non-target organisms. aramove 50 will be contained either in a secured well boat or tarpaulins during application to farmed fish, which will prevent adverse impact to non-target organisms and water quality. rior to tarpaulin application, all nets ropes, and other in-water infrastructure will be pressure washed and thoroughly cleaned to remove any organic material. his will help to reduce the total amount of aramove 50 to be used during treatments and prevent adverse impacts to non-target organisms. itration will occur at several locations within the tarp to monitor the concentration of aramove 50 every five minutes during treatment. fter the tarpaulins are removed the water quality within the net pen will be tested at two and five minutes to ensure concentration of aramove 50 has reduced to 0ppm. Five minutes following treatment, water quality will be tested at four sampling stations 30 m from the sides of the farm closest to the treated pen to confirm that there are no detectable levels of aramove 50, thereby preventing adverse impacts to water quality and non-target organisms. he four sampling stations will be tested at 1m, 5m, and 10m depths. raining taff that will help lead the application of aramove 50 will be Barry illigan (Fish ealth irector and eterinarian), German ampos (General anager of perations), and Brock homson (irector of perations). German ampos, Brock homson, and Barry illigan are familiar with the application methods and requirements as well as the monitoring protocols. he following training has been provided to ensure the safe and effective application of aramove 50, thus preventing adverse impacts to water quality on human health, or detrimental effects to the environment and wildlife. ydrogen eroxide ( 2 2 ) pplication for the treatment of ea ice

13 ermaq anada td eptember 2017 ourse escription rovided by articipants ate ealth and afety 22 handling and awareness an rmstrong, ourse upervisor, qua harma td. ay 2016 esticide pplicators ertification General afety and quipment raining hysical and chemical properties of 22, first aid and health standards, decomposition, safe handling and storage, acceptable and unacceptable materials to use, equipment requirements, procedures for accidents, spills, fire. afe transfer and filling procedures for containers esigned for nterox aramove 50 application for sea lice treatment in marine environments afe handling, storage, transport and administration of aramove 50 rt Guite B.c.,..,.g., () ermaq anada perations irector, rea roduction anager(s), Fish ealth echnician Barry illigan (Fish ealth irector & eterinarian) James ostello (rea roduction anager) Jason earson (rea roduction anager) ll staff involved in the treatment and present at site during treatment arch 2017 ydrogen eroxide ( 2 2 ) pplication for the treatment of ea ice

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