Ca lc ineur in signa l tran sduction pa thway involves in card iom yocyte hyper2 trophy induced by prostagland in F 2

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1 1272 Chinese Journal of Pathophysiology 2007, 23 (7) : [ ] (2007) PGF 2 3 2, 1, 2, 1, 1, 1, 1 ( 1, ; 2,400016) [ ] : F 2 ( PGF 2 ) :, (ANF) mrna,pgf 2 Fura - 2 /AM ( [ Ca 2 + ] i ) ; RT - PCR mrna;w estern blotting :PGF 2 ( mol/l), [ Ca 2 + ] i ( P 0101) ; PGF mol/l ANF mrna ( P 0101) ; (CaN) mrna CaN ( CaN NFAT 3 GATA 4 ) ( P 0105) CsA (CaN ), PGF 2 [ Ca 2 + ] i ( P 0101), CaN mrna CaN( P 0105) : PGF 2 [ Ca 2 + ] i CaN [ ] F ; ; ; [] R363[] A Ca lc ineur in signa l tran sduction pa thway involves in card iom yocyte hyper2 trophy induced by prostagland in F 2 J IANG Q ing - song 2, HUANG Xie - nan 1, ZHOU Q i - xin 2, YANG Gui - zhong 1, DA I Zhi - kai 1, WU Q in 1, SH I J ing - shan 1 ( 1 D epartm ent of Pharm acology, ZunyiM edical College, Zunyi , China; 2 D epartm ent of Pharm acology, Chongqing M edical U niversity, Chongqing , China. E - m ail: huangxienan@ yahoo. com. cn) [ ABSTRACT] A IM : To study the role of p rostaglandin F 2 ( PGF 2 ) in cardiac hypertrophy and its relation with calcineurin (CaN) signal transduction pathway in vitro. M ETHOD S: The cultured neonatal rat cardiomyocyte was used to observe the hypertrophic effect of PGF 2, and the hypertrophic response was assayed by measuring the cell diameter, p ro2 tein content and atrial natriuretic factor (ANF) mrna exp ression. For mechanism studies, the intracellular free calcium concentration ( [ Ca 2 + ] i ) in cultured cardiomyocytes was measured by using Fura - 2 /AM as a fluorescent indicator. ANF and CaN mrna exp ressions, and the exp ressions of CaN and its downstream effectors, NFAT 3 and GATA 4 p roteins were assayed by RT - PCR and W estern blotting, respectively. RESUL TS: In cultured cardiomyocytes, PGF 2 induced p rofound hypertrophic morphology change and the significant increase in cell diameter, and p rotein content in a concentration - de2 pendent manner compared with those in vehicle control ( P < 0101). The same result was found in measuring the [ Ca 2 + ] i in cardiomyocytes ( P < 0101). PGF 2 at concentration of 10-7 mol/l significantly p romoted ANF and CaN mrna exp res2 sions and the p rotein exp ressions of CaN /NFAT 3 /GATA 4 compared with those in the vehicle control ( P < 0105). Cyclos2 porin A, a CaN inhibitor, markedly inhibited the myocyte hypertrophy ( P < 0101), reduced the increased [ Ca 2 + ] i ( P < 0101), and decreased the exp ressions of CaN mrna and CaN /NFAT 3 /GATA 4 p roteins ( P < 0105) compared with those of only PGF mol/l treatment. CO NCL US IO N: Cardiomyocyte hypertrophy induced by PGF 2 may be, at least in part, mediated by CaN signal transduction pathway activated by increasing [ Ca 2 + ] i. [ KEY WO RD S] Prostaglandins F; Myocardial hypertrophy; Signal transduction; Calcineurin [ ] [ ] [ ] [No. (2004) 3057 ] Tel: ; E - mail: huangxienan@ yahoo. com. cn

2 1273 F 2 ( p rostaglandin F 2, PGF 2 ) CaCl mmol/l MgCl mmol/l, [ 1, 2 ], PGF 2 PGF 2,, F( FP ) Gq, Gq, ) ( ) 10 8 C (p rotein kinase C, PKC) p38 ( p38 m itogen - activated p rotein kinase, p38 MAPK) ( extracellular - regu2 lated kinase, ERK) [ 3 ], 2 d011 mmol/l 5 - B r2 PGF 2 PGF 2 FP, C ( phospholipase C, PLC), 72 h,,( diacylglycerol, DAG) PKC,1, 4, 5 - ( inositol triphosphate, IP 3 ) Ca 2 +, 95%, [ 4 ] Ca ( calci2 neurin, CaN)? PGF 2, 4% 15 m in,,,pgf 2 CaN, ), d SD,R IPA, ( : SCXK ( ) ) : PGF 2 (Cayman Chem ical) ; DMEM ( Gibco) ;,- 70 ( ) ; ( ) ; Triton X ( ) ; R IPA (Up state) ; B radford () ; RNA (Q iagen Company) ; RT - PCR ( Promega) ; A ( cyclosporin A, CsA ) Fura - 2 /AM EGTA 5 - ( 5 - B rdu ) Sigma ; CaN,: ANF, sense: 5 - GCC NFAT 3 GATA 4 Santa Cruz D - Hanks(mmol/L ) : NaCl 13710, KCl 510, Na 2 HPO 4 016, KH 2 PO 4 014, NaHCO 3 310, glucose 516 ph D - Hanks Hanks 2 20%DMEM, (115-3) 10 9 cells/l,25 ml ( cells/l 6( ) 24( ),37 5% CO h, 48 h du 48 h 48 h, - actin 3, D - Hanks HE B I2000(,10-15, %, g 4 20 m in, 5 RT - PCR ( a tr ia l na tr i2 uretic factor, ANF) CaN m RNA RNA, RT - PCR ANF CaN mrna [ 5, 6 ] GenBank CTG AGC GAG CAG ACC GA - 3, antisense: 5 - CGG AAG CTG TTG CAG CCT A - 3 ; CaN, sense: 5 - ACT GGC ATG CTC CCC AGC GGA - 3, anti2 sense: 5 - GTG CCG TTA GTC TCT GAG GCG - 3 ; - actin, sense: 5 - GAC TAC CTC ATG AAG ATC

1274 CTG ACC - 3, antisense: 5 - TGA TCT TCA TGG TGC TAG GAG CC - 3 202 bp 244 bp 423 bp RT - PCR ANP CaN mrna, : ( 1 ) : 48 45 m in, 94 2 m in, 1 (2) : 94 30 s, 60 30 s, 72 1 m in, 35 ( 3) : 3 8 gx

3 1274 CTG ACC - 3, antisense: 5 - TGA TCT TCA TGG TGC TAG GAG CC bp 244 bp 423 bp RT - PCR ANP CaN mrna, : ( 1 ) : m in, 94 2 m in, 1 (2) : s, s, 72 1 m in, 35 ( 3) : 3 8 gx s, SPSS1115 ANOVA t 1 PGF 2 C sa HE, PGF 2 ( m in B I2000 ( ),ANF CaN- actin 10-5 mol/l),,, ( 1A B) (A ), 1, 3 PGF 2, ( [ Ca 2 + ] i ) [ 7] 01125%,5 mol/l Fura - 2 /AM, Hanks, cells/l, 10-5 mol/l ( 10 ) ( P 0101), PGF 2, ( )(, PGF mol/l 500 nm, 5 nm 10 nm, CsA 10-6 mol/l PGF nm ) [ Ca 2 + ] i = Kd ( F mol/l ( 1C 2) F m in ) / ( F max - F)[ Ca 2 + ] i 7 W estern blotting CaN NFAT 3 GATA 4 30 g, ( 2), 42% ( P 0101) 23% ( P 0101) ANF mrna PGF mol/l ANF mrna PVDF, ( CaN: ( P 0101 ) CsA ; NFAT 3 : ; GATA 4 : PGF 2 ANF( P 0101) ( 2) ),, Fig 1 The HE staining of cardiomyocytes treated with (B) orwithout (A) PGF mol/l and treated with PGF mol/l p lus CsA 10-6 mol/l (C). 1HE

4 PGF 2 [ Ca 2 + ] i C sa,[ Ca 2 + ] i ( ) nmol/l,10 PGF 2 ( mol/l )48 h, mol/l, [ Ca 2 + ] i ( [Ca 2 + ] i, P 0101) PGF mol/l [ Ca 2 + ] i ( ) nmol/l, CsA 10-6 mol/l PGF mol/l, [ Ca 2 + ] i ( ) nmol/l ( P 0101), 2 1 PGF 2 Tab 1 Effects of PGF 2 (mol/l ) on the diameters and p rotein levels of cardiomyocytes Fig 2 Effects of PGF 2 at different concentrations on the intra2 cellular free calcium concentration ( [ Ca 2 + ] i ) of cardiac myocytes and the inhibition of CsA ( 10-6 mol/l ) on the elevated [ Ca 2 + ] i induced by PGF mol/l vs control; ## P 0101 vs PGF mol/l. 3 3 P < 2PGF 2 Ca 2 + ( [ Ca 2 + ] i ) C sa 4 PGF 2 CaN /NFAT 3 /GATA 4 Group Control PGF PGF PGF PGF PGF PGF Cell diameter ( m) ( n = 3) Protein level (pg/cell) ( n = 6) C sa 1,PGF mol/l, CaN /NFAT 3 /GATA ( P 0105) CsA 10-6 mol/l PGF 2 CaN NFAT 3 GATA 4, ( P 0105) 3 3 P < vs control. 2 C sa PGF m ol/l ( ) ANF m RNA Tab 2 Effects of cyclosporin A (CsA) on the elevated cell diame2 ter, p rotein level and ANF mrna exp ression of cardio2 myocytes induced by PGF 2 at 10-7 mol/l Cell diameter Protein level ANF mrna NFAT 3 GATA 4 Group ( m) (n =3) (pg/cell) (n =6) (n =3),PGF 2 Gq - Control CaN PGF CaN PGF PGF CsA ## ## ## 2, 3 3 ANF: atrial natriuretic factor. P < 0101 vs control; ## P < CsA, CsA 0101 vs PGF mol/l treatment. PGF 2, 3 PGF 2 CaN m RNA C sa, mrna CaN mrna - ac2 tin , PGF mol/l CaN mrna,ca ( P 0101) CsA 10-6 mol/l PGF 2, CaN mrna ( P 0101) [ 8, 9 ], Ca 2 + /CaM CaN, PGF 2,,CaN mrna CaN, NFAT 3 GA2 TA 4,CsA PGF 2 CaN PGF 2 - CaN,

5 1276, regulation of p rostaglandin F 2 recep tor isoform s by p rotein, kinase C [ J ] Mol Pharmacol, 2000, 57 ( 2 ) : 353 -, - 1 [ 5 ] Taigen T, DeW indt JJ, Molkentin JD. Targeted inhibition of calcineurin p revents agonist - induced cardiomyocyte PKC, - 3(phos2 hypertrophy [ J ]. Proc Natl Acad Sci USA, 2000, 97 phatidylinositol 3 - kinase, P I 3 K) MAPK (3) : [ ] [ 6 ] Abassi Z, B rodsky S, Gealekman O, et al. Intrarenal ex2,, CsA PGF 2, CaN, PGF 2, PGF 2 MAPK 3 JNK [ 3 ] p ression and distribution of cyclooxygenase isoform s in rats with experimental heart failure [ J ]. Am J Physiol, 2001, 280 (1) : F43 - F53. [ 7 ],,,. [ J ]., 2002, 25, Ca CaN (2) : [ 8 ] Molkentin JD. Calcineurin and beyond: cardiac hyper2 JNK, PGF 2 trophic signaling [ J ]. Circ Res, 2000, 87 ( 9 ) : 731 -, 738. [ ] [ 9 ] Molkentin JD, Dorn II GW 2nd. Cytop lasm ic signaling pathways that regulate cardiac hypertrophy [ J ]. Annu Rev [ 1 ] Lai J, J in H, Yang R, et al. Prostaglandin F 2 induces Physiol, 2001, 63 (1) : cardiac myocyte hypertrophy in vitro and cardiac growth in vivo [ J ]. Am J Physiol, 1996, 271 ( 6 Pt 2) : H H2208. [ 2 ] Adam s JW, M igita DS, Yu MK, et al. Prostaglandin F2 alpha stimulates hypertrophic growth of cultured neonatal rat ventricular myocytes [ J ]. J B iol Chem, 1996, 271 (2) : [ 3 ] Adam s JW, Sah VP, Henderson SA, et al. Tyrosine ki2 nase and c - jun NH 2 - term inal kinase mediate hyper2 trophic response to p rostaglandin F 2 in cultured neonatal rat ventricular myocytes [ J ]. Circ Res, 1998, 83 ( 2 ) : [ 4 ] Fujino H, Srinivasan D, Pierce KL, et al. D ifferential [ 10 ] W ang L, Gout I, Proud CG. Cross - talk between the ERK and p70 S6 kinase ( S6K) signaling pathways [ J ]. J B iol Chem, 2001, 276 (35) : [ 11 ] Yue TL, Gu JL, W ang C, et al. Extracellular signal - regulated kinase p lays an essential role in hypertrophic ag2 onists, endothelin - 1 and phenylephrine - induced car2 diomyocyte hypertrophy [ J ]. J B iol Chem, 2000, 275 (48) : [ 12 ] Sugden PH. Signaling pathways activated by vasoactive pep tides in the cardiac myocyte and their role in myocar2 dial pathologies [ J ]. J Card Fail, 2002, 8 ( 6 Supp l) : S359 - S369.

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