Aqueous GPC Eluents and Calibrations
|
|
- Priscilla Phelps
- 5 years ago
- Views:
Transcription
1 Eluent Name: Sodium 4-Styrenesulfonate Eluent Polymer Types: NaStS Eluent Composition: 70 % 0.2M NaNO 3, 0.01M NaH 2 PO 4, at ph 7.0; 30 % methanol Column(s): PL Aquagel-OH 40 and PL Aquagel-OH 30 Flow Rate: 1.0 ml/min Sample : 5 to 10 mg/ml Ingredient Amount 1 Sodium nitrate 23.8 g 2 Sodium dihydrogen phosphate 1.68 g 3 Distilled water 1400 ml 4 HPLC grade methanol 600 ml Use 630 ml as excess 5 5M NaOH to ph 7.0 Approx. 2.2 to 2.4 ml required 1 Weigh out two salts and dissolve in water. 2 Adjust to ph 7.0 with NaOH. 4 Degas an excess of HPLC grade methanol for 30 minutes, then add to aqueous phase. 5 Degas for an additional 5 to 10 minutes. Calibration Polymer Type: NaStS homopolymers Number of Calibrants: 10 M p Range: 1,100 to 208, ,100 1 Higher conc. to clear solvent peaks 2 2,290 1 Higher conc. to clear solvent peaks 3 4,480; 29,500; 148,500 8 mg/ml 4 6,430; 45,100; 208,000 8 mg/ml 5 15,800; 86,500 8 mg/ml Information Supplied By: Peter Iddon Last Updated: 17 September 2003 Last Printed: 03/11/2004 Page 1 of 6
2 Eluent Name: TRISMA (new) Polymer Types: MPC Eluent Composition: 0.2M NaNO 3, 0.05M TRISMA buffer, at ph 7.0 Column(s): PL Aquagel-OH 40 and PL Aquagel-OH 30 Flow Rate: 0.5 ml/min Sample : 5 to 10 mg/ml Maximum Run Length: 45 minutes Calibration Polynomial: 3rd Order Ingredient Amount 1 Sodium nitrate 34.0 g 2 Tris(hydroxymethyl)aminomethane 6.06 g 3 Tris(hydroxymethyl)aminomethane.HCl 7.88 g The HCl salt 4 Distilled water 2000 ml 5 HCl to ph Dissolve the three salts in the water. 2 Adjust to ph 7.0 with HCl. 3 Degas for 1 hour under house vacuum. Calibration Polymer Type: PEO homopolymers Number of Calibrants: 10 M p Range: 400 to 78, mg/ml ; 4,120; 22,450 1,080; 7,100 1,470; 12,600 55,600 78,450 Heating of the higher molecular weight calibrants is required to properly dissolve them. However, excessive heating can degrade the polymers. Information Supplied By: Peter Iddon Last Updated: 17 September 2003 Last Printed: 03/11/2004 Page 2 of 6
3 Incomplete Data Entered! Eluent Name: TRISMA (old) Polymer Types: MPC Eluent Composition: 0.2M NaNO 3, 0.05M TRISMA buffer, at ph 8-10 Column(s): Pharmacia Biotech Superose 6 Flow Rate: unknown Sample : unknown mg/ml Maximum Run Length: unknown Calibration Polynomial: unknown Order Ingredient Amount 1 Sodium nitrate 34.0 g 2 Tris(hydroxymethyl)aminomethane 6.06 g 3 Tris(hydroxymethyl)aminomethane.HCl 7.88 g The HCl salt 4 Distilled water 2000 ml 1 Dissolve the three salts in the water. 2 Adjust to ph Degas for 1 hour under house vacuum. Calibration Polymer Type: PEO homopolymers Number of Calibrants: unknown M p Range: unknown Injection Calibrant M p Value(s) Heating of the higher molecular weight calibrants is required to properly dissolve them. However, excessive heating can degrade the polymers. Heat the PEO solutions to 40 C for 1 hour only. Information Supplied By: Iris Ma Last Updated: 18 November 2003 Last Printed: 03/11/2004 Page 3 of 6
4 Eluent Name: ph 9 with 30 % methanol Polymer Types: SEMA, GMA Eluent Composition: 70 % 0.2M NaNO 3, 0.01M NaH 2 PO 4, at ph 9; 30 % methanol Column(s): PL Aquagel-OH 40 and PL Aquagel-OH 30 Flow Rate: 1.0 ml/min Sample : 5 to 10 mg/ml Ingredient Amount 1 Sodium nitrate 23.8 g 2 Sodium dihydrogen phosphate 1.68 g 3 Distilled water 1400 ml 4 HPLC grade methanol 600 ml Use 630 ml as excess 5 5M NaOH to ph 9 1 Weigh out two salts and dissolve in water. 2 Adjust to ph 9 with NaOH. 4 Degas an excess of HPLC grade methanol for 30 minutes, then add to aqueous phase. 5 Degas for an additional 5 to 10 minutes. Calibration Polymer Type: PEO homopolymers Number of Calibrants: 10 M p Range: 400 to 78, mg/ml ; 4,120; 22,450 1,080; 7,100 1,470; 12,600 55,600 78,450 Information Supplied By: Peter Iddon Last Updated: 17 September 2003 Last Printed: 03/11/2004 Page 4 of 6
5 Eluent Name: Acidic Eluent Polymer Types: Cationic polymers (e.g. DMA, DEA, DPA) Eluent Composition: 0.5 M acetic acid, 0.3 M NaH 2 PO 4, at ph 2 Column(s): ViscoGEL G5000 PWxL and ViscoGEL G2500 PWxL Flow Rate: -1 1 mlmin Sample : Ingredient Amount 1 Acetic Acid 60 g 2 Sodium dihydrogen phosphate 72 g 3 Deionised water 2000 ml 1 Weigh out salts and acid and dissolve in water. 2 Adjust to ph 2 using concentrated HCl if necessary. Calibration Polymer Type: Poly(2-vinylpyridine) homopolymers Number of Calibrants: 8 M p Range: 1,480 to 117, ,480; 20, ,200; 50, ,400; 65, ,000; 117,000 Information Supplied By: Peter Iddon Last Updated: 3 November 2004 Last Printed: 03/11/2004 Page 5 of 6
6 Eluent Name: Sugar Methacrylate Monomer Eluent Polymer Types: Polymers based on sugar methacrylate monomers (e.g. LAMA, GAMA) Eluent Composition: 0.1 M NaNO 3, 0.01 M NaH 2 PO 4, at ph 7 Column(s): ViscoGEL alpha 3000 Flow Rate: -1 1 mlmin Sample : Ingredient Amount 1 Sodium nitrate 17.0 g 2 Sodium dihydrogen phosphate 2.4 g 3 Deionised water 2000 ml 1 Weigh out salts and dissolve in water. 2 Adjust to ph 7 using 5 M NaOH. Calibration Polymer Type: Poly(ethylene oxide) homopolymers Number of Calibrants: 10 M p Range: 400 to 78, mg/ml 2 620; 4,120; 22, ,080; 7, ,470; 12, , ,450 Information Supplied By: Peter Iddon Last Updated: 6 October 2004 Last Printed: 03/11/2004 Page 6 of 6
No.106. Aqueous SEC Columns for Analysis of Cationic Polymers: TSKgel PWXL-CP Series. Contents. Page
No.106 Aqueous SEC Columns for Analysis of Cationic Polymers: TSKgel PWXL-CP Series Contents Page 1. Introduction 2. Features 3. Basic characteristics 3-1. Pore characteristics 3-2. Sample injection volume
More informationGFC separation of water-soluble polymers with TSKgel PW-type columns (2) using the PWXL series. Contents
No.038 GFC separation of water-soluble polymers with TSKgel PW-type columns (2) using the PWXL series Contents Page 1. Introduction 2. Calibration curves, theoretical plate numbers and separation range
More informationBCH312 [Practical] 1
BCH312 [Practical] 1 Triprotic acid is acid that contain three hydrogens ions. It dissociates in solution in three steps, with three Ka values. phosphoric acid is an example of triprotic acid. It dissociates
More informationNow, the excess strong base will react: HA + OH - A - + H 2 O Start mol End mol
Chemistry Spring 016 Exam 3: Chapters 8-10 Name 80 Points Complete problem 1 and four of problems -6. CLEARLY mark the problem you do not want graded. You must show your work to receive credit for problems
More informationANALYTICAL METHOD PROCEDURES
HPLC ASSAY AND RELATED SUBSTANCE Column Eurospher 100, C18, 25 x 0.40 cm 5µ Mobile Phase Buffer ph 2.0*: Acetonitrile (88:12 v/v) * Buffer ph 2 Potassium dihydrogen phosphate (KH 2 PO 4 ) - 0.68g Hepatane
More informationGFC Analysis of Water-Soluble Polymers with TSKgel PW-type Columns. Contents
No.035 GFC Analysis of Water-Soluble Polymers with TSKgel PW-type Columns Contents Page 1. Introduction 2. Separation ranges for each grade of TSKgel PW-type column 3. GFC apparatus for water-soluble systems
More informationAnalysis of Metals, Halides, and Inorganic Ions Using Hydrophilic Interaction Chromatography
Application Note Inorganic Ions, Water Testing, Minerals, Metals, Basic Chemicals Analysis of Metals, Halides, and Inorganic Ions Using Hydrophilic Interaction Chromatography Authors Anne Mack, Adam Bivens
More informationQuick guide to selecting columns and standards for Gel Permeation Chromatography and Size Exclusion Chromatography SELECTION GUIDE
Quick guide to selecting columns and standards for Gel Permeation Chromatography and Size Exclusion Chromatography SELECTION GUIDE Introduction Gel permeation chromatography (GPC) and size exclusion chromatography
More informationph calculations MUDr. Jan Pláteník, PhD Brønsted-Lowry concept of acids and bases Acid is a proton donor Base is a proton acceptor
ph calculations MUDr. Jan Pláteník, PhD Brønsted-Lowry concept of acids and bases Acid is a proton donor Base is a proton acceptor HCl(aq) + H 2 O(l) H 3 O + (aq) + Cl - (aq) Acid Base Conjugate acid Conjugate
More informationChemistry 1B Experiment 11 49
Chemistry 1B Experiment 11 49 11 Buffer Solutions Introduction Any solution that contains both a weak acid HA and its conjugate base A in significant amounts is a buffer solution. A buffer is a solution
More information7. Stability indicating analytical method development and validation of Ramipril and Amlodipine in capsule dosage form by HPLC.
7. Stability indicating analytical method development and validation of and in capsule dosage form by HPLC. 7.1 INSTRUMENTS AND MATERIALS USED 7.1.1 INSTRUMENTS 1. Shimadzu LC-2010 CHT with liquid chromatograph
More informationChemistry 222. Start mol End mol
Chemistry Spring 019 Exam 3: Chapters 8-10 Name 80 Points Complete problem 1 and four of problems 6. CLEARLY mark the problem you do not want graded. You must show your work to receive credit for problems
More informationElectronic Supplementary Information : assembly of ph responsive branched copolymer-stabilised emulsion via electrostatic forces
Electronic Supplementary Material (ESI) for ChemComm. This journal is The Royal Society of Chemistry 01 Electronic Supplementary Information : Reversible assembly of ph responsive branched copolymer-stabilised
More informationAcid-Base Equilibria and Solubility Equilibria
Acid-Base Equilibria and Solubility Equilibria Acid-Base Equilibria and Solubility Equilibria Homogeneous versus Heterogeneous Solution Equilibria (17.1) Buffer Solutions (17.2) A Closer Look at Acid-Base
More informationPreparation Of Buffer Solutions By Different Laboratory Ways. BCH 312 [Practical]
Preparation Of Buffer Solutions By Different Laboratory Ways BCH 312 [Practical] Dissociation of Triprotic acid Triprotic acid is acid that contain three hydrogens ions. It dissociates in solution in three
More informationMaking Buffers v010417
Making Buffers v010417 Purposes of the Experiment Make a buffer with a desired ph. Demonstrate the buffering capacity of this buffer. Background Buffers are solutions containing a mixture of a weak acid
More informationBuffer Preparation. Learning Objectives:
Proteomics Buffer Preparation Buffer Preparation Maintaining the optimum ph during the biological sample processing is to maintain the proper functional and structural aspects of the sample. It is important
More informationCh. 4 In-Class Exercise. sodium chloride, glucose, ethanol, lead nitrate, sucrose, methanol
Chemistry 121 Ch. 4 In-Class Exercise In this exercise we will discuss solutions. Since water is the most common solvent, we will focus on aqueous solutions. Solutes (the minor components of solutions)
More informationAP Chemistry: Acid-Base Chemistry Practice Problems
Name AP Chemistry: Acid-Base Chemistry Practice Problems Date Due Directions: Write your answers to the following questions in the space provided. For problem solving, show all of your work. Make sure
More informationCHAPTER 4 TYPES OF CHEMICAL REACTIONS & SOLUTION STOICHIOMETRY
Advanced Chemistry Name Hour Advanced Chemistry Approximate Timeline Students are expected to keep up with class work when absent. CHAPTER 4 TYPES OF CHEMICAL REACTIONS & SOLUTION STOICHIOMETRY Day Plans
More informationINTRODUCTORY CHEMISTRY Concepts and Critical Thinking Seventh Edition by Charles H. Corwin
Lecture INTRODUCTORY CHEMISTRY Concepts and Critical Thinking Seventh Edition by Charles H. Corwin Acids and Bases Properties of Acids An acid is any substance that releases hydrogen ions, H +, into water.
More informationTYPES OF CHEMICAL REACTIONS
TYPES OF CHEMICAL REACTIONS Precipitation Reactions Compounds Soluble Ionic Compounds 1. Group 1A cations and NH 4 + 2. Nitrates (NO 3 ) Acetates (CH 3 COO ) Chlorates (ClO 3 ) Perchlorates (ClO 4 ) Solubility
More informationInt. J. Pharm. Sci. Rev. Res., 30(2), January February 2015; Article No. 09, Pages: 63-68
Research Article Stability indicating RP-HPLC Method for Determination of FexoFenadine Hydrochloride and its Related Substances in Active Pharmaceutical Substance Abhay Gupta* 1, Dr. Birendra Srivastava,
More informationDetermination of Carbonyl Compounds In Water by Dinitrophenylhydrazine Derivatization and HPLC/UV*
Determination of Carbonyl Compounds In Water by Dinitrophenylhydrazine Derivatization and HPLC/UV* EPA Method 8315A UCT Part Number: EUC1812M15 (Unendcapped C18-2000 mg/15 ml cartridge) March 2013 Method
More informationChapter 4: Types of Chemical Reactions and Solution Stoichiometry
Chapter 4: Types of Chemical Reactions and Solution Stoichiometry 4.1 Water, the Common Solvent 4.2 The Nature of Aqueous Solutions: Strong and Weak Electrolytes 4.3 The Composition of Solutions (MOLARITY!)
More informationVolume NaOH Delivered (ml)
Chemistry Spring 011 Exam 3: Chapters 8-10 Name 80 Points Complete five (5) of the following problems. Each problem is worth 16 points. CLEARLY mark the problems you do not want graded. You must show your
More informationDraft Method proposal: determination of glucoheptonic acid (HGA) in fertilizers.
Draft Method proposal: determination of glucoheptonic acid (HGA) in fertilizers. 1 Scope This document describes a chromatographic method which allows the identification as well as the determination of
More informationOptimizing GPC Separations
Optimizing GPC Separations Criteria for Solvent Selection True sample solubility (Polarity and Time dependant) Compatibility with columns Avoid non-size exclusion effects (eg adsorption by reverse phase
More informationDr. Rogers Chapter 3 Homework Chem 111 Fall From textbook: 1-23 odd,27,28,29-53 odd,57,59,61,63,65,67,69,71,73,75,77,79 and 81
Dr. Rogers Chapter 3 Homework Chem 111 Fall 2003 From textbook: 1-23 odd,27,28,29-53 odd,57,59,61,63,65,67,69,71,73,75,77,79 and 81 1. Give the name for the following compounds and state whether they are
More informationPolymerisation of Sodium 4-Styrenesulfonate via Atom Transfer Radical Polymerisation
Polymerisation of Sodium 4-Styrenesulfonate via Atom Transfer Radical Polymerisation Peter D. Iddon, Kay L. Robinson and Steven P. Armes ACS Philadelphia Meeting August 2004 Email: P.Iddon@shef.ac.uk Introduction
More informationBuffers and chemicals
Buffers and chemicals A. Commonly Used Buffers Buffer Synonyms pk Molecular weight Phosphate (pk 1 ) 2.12 98.0, free acid Glycine-HCl 2.34 111.53 Citrate (pk 1 ) 3.14 192.1, free acid Formate 3.75 68.0,
More informationDetermination of Trace Cations in Power Plant Waters Containing Morpholine
Application Note 8 Determination of Trace Cations in Power Plant Waters Containing Morpholine INTRODUCTION Morpholine and ammonium are used as additives in power plant waters. Morpholine acts as a corrosion
More informationOMCL Network of the Council of Europe QUALITY MANAGEMENT DOCUMENT
OMCL Network of the Council of Europe QUALITY MANAGEMENT DOCUMENT PA/PH/OMCL (11) 04 QUALIFICATION OF EQUIPMENT ANNEX 1: QUALIFICATION OF HPLC EQUIPMENT Full document title and reference Document type
More information2.) Calculate the ph of a 0.641M solution of barium hydroxide. Assume that pk w = for the conditions under which the solution was prepared.
www.tutor-homework.com (for tutoring, homework help, or help with online classes) 1.) The ph of a weak BASE dissolved in water is 9.14. The concentration of the base is 0.408 M. In addition, to make things
More informationCopyright 2018 Dan Dill 1
TP The expression for the equilibrium constant for the solubility equilibrium M 2 X 2 M X 2 is 1. sp 2 M X 2 / M 2 X 2. sp 2 M 2 X 2 / M 2 X 3. sp 2 M 2 X 2 4. sp M 2 X 2 Lecture 21 CH102 A1 (MWF 9:05
More informationShodex TM ODP2 HP series columns
HPLC Columns Shodex TM ODP2 HP series columns Better retention of highly polar substances Technical notebook No. 6 Contents 1. Introduction 1-1. Specifications 1-2. Eluent Compatibility of ODP2 HP Series
More informationCHEM 60 Spring 2016 Exam 2 Ch 5-8, 100 points total.
Name Exam No. F CHEM 60 Spring 2016 Exam 2 Ch 5-8, 100 points total. Multiple Choice. (20 questions, 3 points each = 60 points total) Mark the letter on the scantron form corresponding to the one best
More informationMethods of purification
Methods of purification Question Paper 1 Level IGSE Subject hemistry (0620/0971) Exam oard ambridge International Examinations (IE) Topic Experimental techniques Sub-Topic Methods of purification ooklet
More informationIons in Solution. Solvent and Solute
Adapted from Peer-led Team Learning Begin at the beginning and go on till you come to the end: then stop." Early ideas of atoms and compounds, developed primarily through the reactions of solids and gases,
More informationph = log[h + ] pk a = logk a = 4.75 K a = HA H + + A K a = [H+ ][A ] [HA] Acid-Base Chemistry: Alpha Fractions, Titrations, Exact Solutions
I. Monoprotic Weak Acid Alpha Fractions HA = Acetic Acid: CH3COOH pk a = logk a = 4.75 K a = 10 4.75 HA H + + A K a = [H+ ][A ] [HA] Two Acid Species: HA and A - Alpha Fractions only depend on ph and pka
More informationCHM112 Lab Hydrolysis and Buffers Grading Rubric
Name Team Name CHM112 Lab Hydrolysis and Buffers Grading Rubric Criteria Points possible Points earned Lab Performance Printed lab handout and rubric was brought to lab 3 Initial calculations completed
More informationHigh Performance Liquid Chromatography
Updated: 3 November 2014 Print version High Performance Liquid Chromatography David Reckhow CEE 772 #18 1 HPLC System David Reckhow CEE 772 #18 2 Instrument Basics PUMP INJECTION POINT DETECTOR COLUMN
More informationHigh Performance Liquid Chromatography
Updated: 3 November 2014 Print version High Performance Liquid Chromatography David Reckhow CEE 772 #18 1 HPLC System David Reckhow CEE 772 #18 2 1 Instrument Basics PUMP INJECTION POINT DETECTOR COLUMN
More informationCHAPTER V ANALYTICAL METHODS ESTIMATION OF DICLOFENAC. Diclofenac (gift sample from M/s Micro Labs Ltd., Pondicherry)
CHAPTER V ANALYTICAL METHODS ESTIMATION OF DICLOFENAC A UV spectrophotometric method based on the measurement of absorbance at 276nm in phosphate buffer of p H 7.4 was used in the present study of the
More informationPart I: Solubility!!!
Name: Period: Date: KIPP NYC College Prep General Chemistry UNIT 12: Solutions Do Now Review Day Concepts/Definitions (1) A solution is made by completely dissolving 40 grams of KCl(s) in 100 grams of
More informationBuffers. How can a solution neutralize both acids and bases? Beaker B: 100 ml of 1.00 M HCl. HCl (aq) + H 2 O H 3 O 1+ (aq) + Cl 1 (aq)
Buffers How can a solution neutralize both acids and bases? Why? Buffer solutions are a mixture of substances that have a fairly constant ph regardless of addition of acid or base. They are used in medicine,
More informationAP Chemistry. Reactions in Solution
AP Chemistry Reactions in Solution S o l u t i o n s solution: a homogeneous mixture of two or more substances -- The solvent is present in greatest quantity. -- Any other substance present is called a.
More informationName: Period: Date: solution
Name: Period: Date: ID: A Solutions Test A Matching Use the choices below to answer the following 5 questions. a. Hydrogen bond d. Electrolyte b. Polar molecule e. Nonelectrolyte c. Nonpolar molecule 1.
More informationWe need to find the new concentrations of the species in this buffer system. Remember that we also DILUTED the solution by adding 5.0 ml of the HCl.
164 Take 100. ml of the previous buffer (0.05 M tris / 0.075 M tris-hcl), and add 5.0 ml of.10 M HCl. What is the ph of the mixture? The HCl reacts with the tris base, converting it to tris-hcl We need
More informationSupporting Information. Copyright Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, 2007
Supporting Information Copyright Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, 2007 Copyright Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, 2007 Supporting Information for Head-to-Tail Cyclized
More informationSOLUTIONS. Chapter Test B. A. Matching. Column A. Column B. Name Date Class. 418 Core Teaching Resources
16 SOLUTIONS Chapter Test B A. Matching Match each term in Column B to the correct description in Column A. Write the letter of the correct term on the line. Column A Column B 1. the number of moles of
More informationChemistry of Soil E1-1
Experiment 1 Chemistry of Soil E1-1 E1-2 The Task The goal of this experiment is to determine which salts could be added to soil to increase or decrease its ph. Skills At the end of the laboratory you
More informationAP Chemistry Multiple Choice Questions - Chapter 4
1 Which of the following contains 6.00 x 10 16 atoms? a 6.00 x 10 16 H 2 O molecules b 3.00 x 10 16 Cl 2 molecules c 2.00 x 10 16 P 4 molecules d 1.50 x 10 16 CaSO 4 empirical units 4.1 2 How many atoms
More informationClasses at: - Confidence building Problems
PHYSICAL CHEMISTRY by: SHAILENDRA KR. Classes at: - SCIENCE TUTORIALS; Opp. Khuda Baksh Library, Ashok Rajpath, Patna PIN POINT STUDY CIRCLE; House No. 5A/65, Opp. Mahual Kothi, Alpana Market, Patna Confidence
More informationDiquat 1,1 -ethylene-2,2 -bipyridium dibromide salt Paraquat 1,1 -dimethyl-4,4 -bipyridium dichloride salt Initial Preparation
EPA Method 549.2 Revision 1.0 Determination of Diquat and Paraquat in Drinking Water by Liquid-Solid Extraction and High Performance Liquid Chromatography with Ultraviolet Detection* UCT Products: ENVIRO-CLEAN
More informationCHEMISTRY 102 A/E Fall 2008 HOUR EXAM I NAME Dr. Christine Yerkes. A. DO NOT open the exam until you are instructed to do so.
CHEMISTRY 102 A/E Fall 2008 HOUR EXAM I NAME Dr. Christine Yerkes October 1, 2008 SIGNATURE Test Form A QUIZ TA A. DO NOT open the exam until you are instructed to do so. B. The exam has 6 pages. After
More information5. What is the percent ionization of a 1.4 M HC 2 H 3 O 2 solution (K a = ) at 25 C? A) 0.50% B) 0.36% C) 0.30% D) 0.18% E) 2.
Name: Date: 1. For which of the following equilibria does K c correspond to an acid-ionization constant, K a? A) NH 3 (aq) + H 3 O + (aq) NH 4 + (aq) + H 2 O(l) B) NH 4 + (aq) + H 2 O(l) NH 3 (aq) + H
More information**The partially (-) oxygen pulls apart and surrounds the (+) cation. The partially (+) hydrogen pulls apart and surrounds the (-) anion.
#19 Notes Unit 3: Reactions in Solutions Ch. Reactions in Solutions I. Solvation -the act of dissolving (solute (salt) dissolves in the solvent (water)) Hydration: dissolving in water, the universal solvent.
More informationTEMPLATE FOR AN EXAMPLE STANDARD TEST METHOD
APPENDIX V TEMPLATE FOR AN EXAMPLE STANDARD TEST METHOD Validating Chromatographic Methods. By David M. Bliesner Copyright 2006 John Wiley & Sons, Inc. 159 160 APPENDIX V Title: Effective: Document No:
More informationClass XI Chapter 1 Some Basic Concepts of Chemistry Chemistry
Question 1.1: Calculate the molecular mass of the following: (i) H 2 O (ii) CO 2 (iii) CH 4 (i) H 2 O: The molecular mass of water, H 2 O = (2 Atomic mass of hydrogen) + (1 Atomic mass of oxygen) = [2(1.0084)
More informationANNEXURE - I MEDIA AND REAGENTS
ANNEXURE - I MEDIA AND REAGENTS I. ZIEHL-NEELSEN METHOD Ziehl-Neelsen carbol fuchsin stain - Basic fuchsin 5 gm Phenol 25 gm Alcohol (95 % of absolute) 50 ml Distilled water 500 ml The fuchsin was dissolved
More informationSolutions. Experiment 11. Various Types of Solutions. Solution: A homogenous mixture consisting of ions or molecules
Solutions Solution: A homogenous mixture consisting of ions or molecules -Assignment: Ch 15 Questions & Problems : 5, (15b,d), (17a, c), 19, 21, 23, 27, (33b,c), 39, (43c,d),45b, 47, (49b,d), (55a,b),
More informationChem 103 Exam #1. Identify the letter of the choice that best completes the statement or answers the question. Multiple Choice
Chem 103 Exam #1 Multiple Choice Identify the letter of the choice that best completes the statement or answers the question. 1. Which of the following can act as a Bronsted-Lowry base, but not as a Bronsted-Lowry
More informationSupporting Information
Supporting Information Efficient Temperature Sensing Platform Based on Fluorescent Block Copolymer Functionalized Graphene Oxide Hyunseung Yang, Kwanyeol Paek, and Bumjoon J. Kim * : These authors contributed
More informationCHEMISTRY 102 Fall 2010 Hour Exam III. 1. My answers for this Chemistry 102 exam should be graded with the answer sheet associated with:
1. My answers for this Chemistry 10 exam should be graded with the answer sheet associated with: a) Form A b) Form B c) Form C d) Form D e) Form E Consider the titration of 30.0 ml of 0.30 M HCN by 0.10
More informationGPC/SEC standards. Product guide
GPC/SEC standards Product guide Contents Polymer standards for GPC/SEC 3 Agilent EasiVial 5 Agilent EasiCal 8 Polystyrene 9 Polymethylmethacrylate 11 Polyethylene glycol/oxide 12 Other polymer standards
More informationPRACTICAL 3 ph AND BUFFERS
PRACTICAL 3 ph AND BUFFERS ph and Buffers Structure 3.1 Introduction 3.2 ph and Buffers: Basic Concept 3.2.1 ph 3.2.2 Buffers and Buffer Solutions 3.3 Methods for Determining ph Experiment 1: Measurement
More informationSOLUTIONS. Solutions - page
SOLUTIONS For gases in a liquid, as the temperature goes up the solubility goes. For gases in a liquid, as the pressure goes up the solubility goes. Example: What is the molarity of a solution with 2.0
More informationDetermination of Adsorbable Organic Halogen in Wastewater
Determination of Adsorbable Organic Halogen in Wastewater Jingli Hu, Jeff Rohrer, and Kirk Chassaniol Thermo Fisher Scientific, Sunnyvale, CA The world leader in serving science Outline AOX (Adsorbable
More information5 Acid Base Reactions
Aubrey High School AP Chemistry 5 Acid Base Reactions 1. Consider the formic acid, HCOOH. K a of formic acid = 1.8 10 4 a. Calculate the ph of a 0.20 M solution of formic acid. Name Period Date / / 5.2
More informationPHYSICAL CHEMISTRY. by: SHAILENDRA KR. Meq. Approach. Classes at: - Topic: Mole Concept
PHYSICAL CHEMISTRY by: SHAILENDRA KR. asses at: - SCIENCE TUTORIALS; Opp. Khuda Baksh Library, Ashok Rajpath, Patna PIN POINT STUDY CIRCLE; House No. 5A/65, Opp. Mahual Kothi, Alpana Market, Patna Topic:
More informationAcid / Base Properties of Salts
Acid / Base Properties of Salts n Soluble ionic salts produce may produce neutral, acidic, or basic solutions depending on the acidbase properties of the individual ions. n Consider the salt sodium nitrate,
More informationCation Exchange HPLC Columns
Cation Exchange HPLC Columns Hamilton offers seven polymeric packing materials for cation exchange separations. Type Recommended Application(s) PRP-X00 PRP-X00 PRP-X800 HC-0 HC-7 Ca + HC-7 H + HC-7 Pb
More informationWaters. Ion Chromatography
808q Waters Ion Chromatography Method Anion Analysis Using Hydroxide Eluent and Indirect Conductivity Detection and EPA Method B-1011 for Nitrite and Nitrate Using UV Detection 2000 Required Instrumentation:
More information9/24/12. Chemistry Second Edition Julia Burdge. Reactions in Aqueous Solutions
Chemistry Second Edition Julia Burdge 4 Reactions in Aqueous Solutions Copyright (c) The McGraw-Hill Companies, Inc. Permission required for reproduction or display. 1 4 Reactions in Aqueous Solutions
More informationFunsheet 9.1 [VSEPR] Gu 2015
Funsheet 9.1 [VSEPR] Gu 2015 Molecule Lewis Structure # Atoms Bonded to Central Atom # Lone Pairs on Central Atom Name of Shape 3D Lewis Structure NI 3 CF 4 OCl 2 C 2 F 2 HOF Funsheet 9.1 [VSEPR] Gu 2015
More informationSession 8: LECTURE OUTLINE (SECTIONS I1 I4 pp F61 F67)
Session 8: LECTURE OUTLINE (SECTIONS I1 I4 pp F61 F67) I. Elecrolytes a. Soluble substances b. Insoluble substances c. Electrolytes d. Non-Electrolytes e. Ions and electrical conductivity f. Strong and
More informationExperiment 7 Buffer Capacity & Buffer Preparation
Chem 1B Dr. White 57 Experiment 7 Buffer Capacity & Buffer Preparation Objectives To learn how to choose a suitable conjugate acid- base pair for making a buffer of a given ph To gain experience in using
More informationQuick Review. - Chemical equations - Types of chemical reactions - Balancing chemical equations - Stoichiometry - Limiting reactant/reagent
Quick Review - Chemical equations - Types of chemical reactions - Balancing chemical equations - Stoichiometry - Limiting reactant/reagent Water H 2 O Is water an ionic or a covalent compound? Covalent,
More informationProPac WCX-10 Columns
ProPac WCX-10 Columns Guidance for column use Tips to maximize column lifetime ProPac WCX-10 Column Tips and Tricks This guide provides essential information and invaluable guidelines for mobile phases,
More information5 Salts and Solutions
Aubrey High School Chemistry 5 Salts and Solutions Name Period Date / / 5.0 Practice Test You may use a pencil, eraser, and scientific calculator to complete the test. You will be given a periodic table
More informationIB Chemistry Solutions Gasses and Energy
Solutions A solution is a homogeneous mixture it looks like one substance. An aqueous solution will be a clear mixture with only one visible phase. Be careful with the definitions of clear and colourless.
More informationChapter 4. The Major Classes of Chemical Reactions 4-1
Chapter 4 The Major Classes of Chemical Reactions 4-1 The Major Classes of Chemical Reactions 4.1 The Role of Water as a Solvent 4.2 Writing Equations for Aqueous Ionic Reactions 4.3 Precipitation Reactions
More information4. Magnesium has three natural isotopes with the following masses and natural abundances:
Exercise #1. Determination of Weighted Average Mass 1. The average mass of pennies minted after 1982 is 2.50 g and the average mass of pennies minted before 1982 is 3.00 g. Suppose that a bag of pennies
More informationAnnouncements. Please come to the front of the classroom and pick up a Solution Problems worksheet before class starts!
Announcements Please come to the front of the classroom and pick up a Solution Problems worksheet before class starts! Announcements 1. Mid-term grades will be posted soon (just used scaled exam 1 score
More informationFull file at Chapter 2 Water: The Solvent for Biochemical Reactions
Chapter 2 Water: The Solvent for Biochemical Reactions SUMMARY Section 2.1 Summary Water is a polar molecule, with a partial negative charge on the oxygen and partial positive charges on the hydrogens.
More information5. What is the name of the phase transition that occurs when a solid is converted directly into a gas (without going through the liquid phase)?
1. If the volume of a confined gas is doubled while the temperature remains constant, what change (if any) would be observed in the pressure? a. It would be half as large. b. It would double. c. It would
More informationAP Chapter 4: Reactions in Aqueous Solution Name
AP Chapter 4: Reactions in Aqueous Solution Name WarmUps (Show your work for credit) Date 1. Date 2. Date 3. Date 4. Date 5. Date 6. Date 7. Date 8. AP Chapter 4: Reactions in Aqueous Solution 2 WarmUps
More informationSolutions. Why does a raw egg swell or shrink when placed in different solutions?
Solutions 1 Why does a raw egg swell or shrink when placed in different solutions? Classification of Matter 2 Some Definitions 3 If a compound is soluble it is capable of being dissolved. A solution is
More informationChapter 3: Solution Chemistry (For best results when printing these notes, use the pdf version of this file)
Chapter 3: Solution Chemistry (For best results when printing these notes, use the pdf version of this file) Section 3.1: Solubility Rules (For Ionic Compounds in Water) Section 3.1.1: Introduction Solubility
More informationCHEMISTRY 102B Practice Hour Exam I. Dr. D. DeCoste T.A (30 pts.) 16 (15 pts.) 17 (15 pts.) Total (60 pts)
CHEMISTRY 102B Practice Hour Exam I Spring 2016 Dr. D. DeCoste Name Signature T.A. This exam contains 17 questions on 5 numbered pages. Check now to make sure you have a complete exam. You have one hour
More informationChapter 15. Solutions
Chapter 15 Solutions Key Terms for this Chapter Make sure you know the meaning of these: Solution Solute Solvent Aqueous solution Solubility Saturated Unsaturated Supersaturated Concentrated Dilute 15-2
More informationGPC/SEC standards. Product guide
GPC/SEC standards Product guide Contents Polymer standards for GPC/SEC 3 Agilent EasiVial 5 Agilent EasiCal 8 Polystyrene 9 Polymethylmethacrylate 11 Polyethylene glycol/oxide 12 Other polymer standards
More informationPolymer analysis by GPC-SEC. Technical Note. Introduction
Polymer analysis by GPC-SEC Technical Note Introduction Gel Permeation Chromatography (GPC), also referred to as Size Exclusion Chromatography (SEC) is a mode of liquid chromatography in which the components
More informationSection 4: Aqueous Reactions
Section 4: Aqueous Reactions 1. Solution composition 2. Electrolytes and nonelectrolytes 3. Acids, bases, and salts 4. Neutralization ti reactions 5. Precipitation reactions 6. Oxidation/reduction reactions
More informationSection EXAM II Total Points = 150. October 15, Each student is responsible for following directions. Read this page carefully.
Name Chemistry 11100 Test 55 Section EXAM II Total Points = 150 TA Monday, 6:30 PM October 15, 2012 Directions: 1. Each student is responsible for following directions. Read this page carefully. 2. Write
More informationNEW SPECTROPHOTOMETRIC METHODS FOR THE QUANTITATIVE ESTIMATION OF OXOLAMINE IN FORMULATION
NEW SPECTROPHOTOMETRIC METHODS FOR THE QUANTITATIVE ESTIMATION OF OXOLAMINE IN FORMULATION V.PhaniKumar 1 *, CH.Venkata Kishore 2 1 Department of Chemistry, Govt college, Tiruvuru, Krishna District Andhra
More informationPart One: Pure Solutions of Weak Acids, Bases (water plus a single electrolyte solute)
CHAPTER 16: ACID-BASE EQUILIBRIA Part One: Pure Solutions of Weak Acids, Bases (water plus a single electrolyte solute) A. Weak Monoprotic Acids. (Section 16.1) 1. Solution of Acetic Acid: 2. See Table
More informationCHEMISTRY 101 Hour Exam II. Dr. D. DeCoste T.A (30 pts.) 16 (15 pts.) 17 (15 pts.) Total (60 pts)
CHEMISTRY 101 Hour Exam II March 16, 2017 Dr. D. DeCoste Name Signature T.A. This exam contains 17 questions on 5 numbered pages. Check now to make sure you have a complete exam. You have one hour and
More informationReactions in aqueous solutions Precipitation Reactions
Reactions in aqueous solutions Precipitation Reactions Aqueous solutions Chemical reactions that occur in water are responsible for creation of cenotes. When carbon dioxide, CO2, dissolves in water, the
More information