Supplementary Information
|
|
- Esmond Hopkins
- 5 years ago
- Views:
Transcription
1 Supplementry Informtion Coopertion of locl motions in the Hsp90 moleculr chperone ATPse mechnism Andre Schulze 1, Gerti Beliu 1, Dominic A. Helmerich 1, Jonthn Schubert 1, Lurence H. Perl 2, Chrisostomos Prodromou 2 & Hnnes Neuweiler 1, * 1 Deprtment of Biotechnology nd Biophysics, Julius-Mximilins-University Würzburg, Am Hublnd, Würzburg, Germny 2 Genome Dmge nd Stbility Centre, School of Life Sciences, University of Sussex, Flmer, Brighton BN1 9RQ, United Kingdom *Corresponding uthor: hnnes.neuweiler@uni-wuerzburg.de 1
2 Supplementry Tbles Supplementry Results Tble 1: Kinetic prmeters of locl motions of Hsp90 mesured t 25 C. Mutnt Reporter 1 k1 2 k 2 3 k 3 men k b Wild type β-strnd swp 0.59 ± ± ± Wild type ssocition 0.68 ± ± ± Wild type Lid closure ± ± ±0.003 A2W-L160W β-strnd swp c 0.35 ± ± ± ±0,0005 A2W-L160W ssocition 0.72 ± ± ± ± 0.01 A2W-L160W Lid closure ± ± ± A107N β-strnd swp 0.61 ± ± ± ± A107N ssocition 0.84 A107N Lid closure 0.32 ± ± ± ± ± ± ± ±0.08 n nd kn re the reltive fluorescence mplitudes nd observed rte constnts (1/ n in 1/min). Dt represent men vlues ± s.d. of three mesurements. b The men rte constnt ws clculted from the sum of individul time constnts weighted by the respective mplitudes (1/(Σn n). c To monitor domin swp of the TrpZip mutnt A2W-L160W, the fluorophore ws plced in witing position on the second subunit of heterodimeric Hsp90 (E162C). Tble 2: ATPse ctivity of wild-type Hsp90 nd mutnts thereof mesured t 37 C. Mutnt ATPse (min -1 ) Wild type 1.6 ± 0.04 A2W-L160W 0.61 ± 0.09 T101I 0.35 ± 0.06 F349A 0.12 ± 0.02 R380A ND ND; not detectble; dt represent men vlues ± s.d. of three mesurements. 2
3 Tble 3: Kinetics of locl motions of wild-type Hsp90 in the presence of Ah1 mesured t 25 C. Mutnt Reporter + Ah1 1 k1 2 k 2 men k b Wild type β-strnd swp 0.59± ± ± ± ±0.25 Wild type ssocition ± ±0.5 Wild type Lid closure 0.52± ± ± ± ±0.55 F349A β-strnd swp 0.62± ± ± ± ±0.06 F349A ssocition 0.70± ± ± ±0.08 F349A Lid closure ND ND ND ND ND n nd kn re the reltive fluorescence mplitudes nd observed rte constnts (1/ n in 1/min). Dt represent men vlues ± s.d. of three mesurements. b The men rte constnt ws clculted from the sum of individul time constnts weighted by the respective mplitudes (1/(Σn n). ND; not detectble. 3
4 Tble 4: Dynmics of NTD structurl elements mesured by PET-FCS t 25 C N-terminl domin 1 1 (µs) 2 2 (µs) 3 3 (µs) Q14C-A2W 0.67± ± ± Q14C-A2W +ATP ± ±10 Q14C-A2W-L160W A112C-S25W ± ±01 7± A112C-S25W + ATP ± ±1 NM-domin Q14C-A2W 1.03± ± ± ± ±0.8 Q14C-A2W +ATP ± ± ± ± ±0.1 Q14C-A2W +Ah ± ± ± ± ±0.8 A112C-S25W ± ±7 0.27± ±0.4 A112C-S25W + ATP ± ±1 0.21± ±0.1 A112C-S25W + Ah ± ±1 0.45± ±0.1 n nd n re the observed mplitudes nd corresponding time constnts. Dt represent men vlues ± s.d. of three mesurements. 4
5 Supplementry Figures Figure 1 Figure 1: Influence of ADP on conformtionl switches of Hsp90 Fluorescence intensity time trces mesured for () -strnd swp (A2C+E162W), (b) -domin ssocition (E192C-N298W), nd (c)/(d) lid closure (reporters S51C-A110W nd S51W-A110C, respectively). 4 mm ADP ws dded t time t = 0 min. Mesurements were done t 25 C. 5
6 Figure 2 Figure 2: Kinetics of erly remodeling of the lid induced by binding of nucleotide () Representtive fluorescence intensity time trces of reporter S51W-A110C on Hsp90 mesured using stopped-flow fluorescence spectroscopy. Kinetic trnsients were triggered by binding of 0.5 mm ADP (green) nd 0.75 mm ADP (blue). Ech time trce is n verge over three individul shots. Blck lines re single exponentil fits to the dt. We used ADP insted of ATP to solely trigger remodeling of the lid nd to void possible interference from other conformtionl chnges tht could complicte dt nlysis. (b) Observed rte constnts plotted versus concentrtion of ADP. Dt represent men vlues ± s.d. of three mesurements (performed t 25 C). The blck line is liner fit to the dt tht yielded bi-moleculr rte constnt of 1.3±0.2 x 10 5 M -1 s -1. Figure 3 Figure 3: Heterogeneity of kinetics of conformtionl switching Plot of the observed mplitudes nd rte constnts of the individul exponentil phses in multi-exponentil decys of conformtionl switching (see Supplementry Tble 1). Amplitudes (filled brs) nd corresponding rte constnts (ptterned brs) re shown in sme color. Pnels (), (b), nd (c), show kinetics mesured for wild-type Hsp90, the TrpZip construct, nd mutnt A107N, respectively. Dt represent men vlues ± s.d. of three mesurements. 6
7 Figure 4 Figure 4: Influence of binding of AMP-PNP on motion of the lid in isolted NTD nd NMdomin. () Fluorescence intensity time trces mesured for reporter A110C-S51W on the NTD in isoltion, which probes closure of the lid. 2 mm AMP-PNP ws dded t time t = 0 min. (b) The sme mesurement for reporter S51C-A110W on the NM-domin in bsence (blck) nd presence (blue) of 20 µm Ah1. Ah1 ws dded t t = -10 min nd AMP-PNP t t = 0 min. Mesurements were done t 25 C. 7
8 Figure 5 Figure 5: Anlysis of PET-FCS dt using model tht lcks µs exponentil relxtion. (), (b) ACFs recorded from -strnd reporter Q14C-A2W nd from lid reporter A112C-S25W, respectively, on the isolted NTD. (c), (d) ACFs recorded from -strnd reporter Q14C-A2W nd from lid reporter A112C- S25W, respectively, on the NM-domin. In cse of the NTD (() nd (b)), cyn lines re fits to the dt using model for moleculr diffusion contining two single-exponentil relxtions. In cse of the NM-domin ((c) nd (d)), cyn lines re fits to the dt using model for moleculr diffusion contining three singleexponentil relxtions. Red lines re fits to the dt using the sme models but lcking single-exponentil relxtion t µs. These fits yielded rtificilly smll diffusion time constnts due to overlp of time scles of diffusion nd conformtionl fluctutions. Specificlly, the NTD hd diffusion time constnt of 1.7 ms tht ws rtificilly reduced to ms. The NM-domin hd diffusion time constnt of 2.2 ms tht ws rtificilly reduced to 1.9 nd 0.9 ms. 8
SUPPLEMENTARY INFORMATION
doi:.38/nture8499 doi:.38/nture8499 5 6 5 4.5 Firing rte (Hz) -67-65 -66-6 -58 V m (mv) -7-67 -68-66 -64 c Thet power (mv ) -73-69 -7-7 -7.5.8 3....9.9.4.6.6. 9 8 9 8 9 8 9 8 9 8 Supplementry Figure Firing
More informationUNIVERSITY OF MALTA DEPARTMENT OF CHEMISTRY. CH237 - Chemical Thermodynamics and Kinetics. Tutorial Sheet VIII
UNIVERSITY OF MALTA DEPARTMENT OF CHEMISTRY CH237 - Chemicl Thermodynmics nd Kinetics Tutoril Sheet VIII 1 () (i) The rte of the rection A + 2B 3C + D ws reported s 1.0 mol L -1 s -1. Stte the rtes of
More informationSupplementary Figures
Electronic Supplementry Mteril (ESI) for Integrtie Biology. This journl is The Royl Society of Chemistry 214 Supplementry Figures CWound Are µm2 A EGTA Low Clcium 8 1 min. fter wounding Wound + 1 min.
More informationSupplementary Figure 1
Supplementry Figure (nesthetized) (wke) Normlized mplitude.5 Pek width (ms).6.4.2 4 2 2 x 3 Wveform slope Normlized mplitude.5 Pek width (ms).6.4.2 x 3 3 2 Wveform slope c (nesthetized) d (wke) Normlized
More informationSupplementary Information. DNA interaction of non-chelating tinidazole-based coordination compounds. Structural, redox and cytotoxic properties.
Electronic Supplementry Mteril (ESI) for Dlton Trnsctions. This journl is The Royl Society of Chemistry 2018 Supplementry Informtion DNA interction of non-chelting tinidzole-bsed coordintion compounds.
More informationFactors affecting the phonation threshold pressure and frequency
3SC Fctors ffecting the phontion threshold pressure nd frequency Zhoyn Zhng School of Medicine, University of Cliforni Los Angeles, CA, USA My, 9 57 th ASA Meeting, Portlnd, Oregon Acknowledgment: Reserch
More informationSupplementary Material (ESI) for Soft Matter This journal is The Royal Society of Chemistry Supplementary data
Supplementry Mteril (ESI) for Soft Mtter This journl is The Royl Society of hemistry 009 Supplementry dt Quntifying Hydrogel Response using Lser Light Scttering. Joshu M. G. Swnn, Wim Brs b, Jonthn R.
More informationInformation processing via physical soft body. Supplementary Information
Informtion processing vi physicl soft body Supplementry Informtion Kohei Nkjim 1, Helmut Huser 2, o Li 3, Rolf Pfeifer 4,5 1 he Hkubi Center for Advnced Reserch & Grdute School of Informtics, Kyoto University,
More informationThe GlcN6P cofactor plays multiple catalytic roles in the glms ribozyme
SUPPLEMENTARY INFORMATION The GlcN6P cofctor plys multiple ctlytic roles in the glms ribozyme Jmie L. Bingmn 1, Sixue Zhng 2, Dvid R. Stevens 2, Neel H. Yennwr 3, Shron Hmmes-Schiffer 2,* Philip C. Bevilcqu
More informationSUPPLEMENTARY INFORMATION
doi:1.138/nture11444 CMKIIN Gold prticles/ mitochondril re ( m ) 4 3 1 CMKIIN mtcmkiin mtcmkiin SERCA ATP synthse HA mitoplsts mtcmkiin CMKIIN cytosolic mtcmkiin CMKIIN 97 kdl 64 kdl 51 kdl 14 kdl 6 kdl
More informationVertical uniformity of cells and nuclei in epithelial monolayers
Supplementry informtion Verticl uniformity of cells nd nuclei in epithelil monolyers Srujn Neelm b, Peter Hyes, Qio Zhng, Richrd B. Dickinson nd Tnmy P. Lele, Figure S1. Histogrm plots compre the frequency
More informationModule 2: Rate Law & Stoichiomtery (Chapter 3, Fogler)
CHE 309: Chemicl Rection Engineering Lecture-8 Module 2: Rte Lw & Stoichiomtery (Chpter 3, Fogler) Topics to be covered in tody s lecture Thermodynmics nd Kinetics Rection rtes for reversible rections
More informationSimple Harmonic Motion I Sem
Simple Hrmonic Motion I Sem Sllus: Differentil eqution of liner SHM. Energ of prticle, potentil energ nd kinetic energ (derivtion), Composition of two rectngulr SHM s hving sme periods, Lissjous figures.
More informationDepartment of Physical Pharmacy and Pharmacokinetics Poznań University of Medical Sciences Pharmacokinetics laboratory
Deprtment of Physicl Phrmcy nd Phrmcoinetics Poznń University of Medicl Sciences Phrmcoinetics lbortory Experiment 1 Phrmcoinetics of ibuprofen s n exmple of the first-order inetics in n open one-comprtment
More informationSection 14.3 Arc Length and Curvature
Section 4.3 Arc Length nd Curvture Clculus on Curves in Spce In this section, we ly the foundtions for describing the movement of n object in spce.. Vector Function Bsics In Clc, formul for rc length in
More informationScientific notation is a way of expressing really big numbers or really small numbers.
Scientific Nottion (Stndrd form) Scientific nottion is wy of expressing relly big numbers or relly smll numbers. It is most often used in scientific clcultions where the nlysis must be very precise. Scientific
More informationRates of chemical reactions
Rtes of chemicl rections Mesuring rtes of chemicl rections Experimentl mesuring progress of the rection Monitoring pressure in the rection involving gses 2 NO( g) 4 NO ( g) + O ( g) 2 5 2 2 n(1 α) 2αn
More informationPower-Law Behavior of Power Spectra in Low Prandtl Number Rayleigh-Bénard Convection
Power-Lw Behvior of Power Spectr in Low Prndtl umber Ryleigh-Bénrd Convection M. R. Pul nd M. C. Cross Deprtment of Physics, Cliforni Institute of Technology 114-36, Psden, Cliforni 9115 P. F. Fischer
More informationA027 Uncertainties in Local Anisotropy Estimation from Multi-offset VSP Data
A07 Uncertinties in Locl Anisotropy Estimtion from Multi-offset VSP Dt M. Asghrzdeh* (Curtin University), A. Bon (Curtin University), R. Pevzner (Curtin University), M. Urosevic (Curtin University) & B.
More informationThe development of nanoscale morphology in polymer:fullerene. photovoltaic blends during solvent casting
Supplementry informtion Supplementry Mteril (ES) for Soft Mtter The development of nnoscle morphology in polymer:fullerene photovoltic lends during solvent csting To Wng, * Aln D. F. Dunr, Pul A. Stniec,
More informationLECTURE 14. Dr. Teresa D. Golden University of North Texas Department of Chemistry
LECTURE 14 Dr. Teres D. Golden University of North Texs Deprtment of Chemistry Quntittive Methods A. Quntittive Phse Anlysis Qulittive D phses by comprison with stndrd ptterns. Estimte of proportions of
More informationPhysics 201 Lab 3: Measurement of Earth s local gravitational field I Data Acquisition and Preliminary Analysis Dr. Timothy C. Black Summer I, 2018
Physics 201 Lb 3: Mesurement of Erth s locl grvittionl field I Dt Acquisition nd Preliminry Anlysis Dr. Timothy C. Blck Summer I, 2018 Theoreticl Discussion Grvity is one of the four known fundmentl forces.
More informationGeneralized Fano and non-fano networks
Generlized Fno nd non-fno networks Nildri Ds nd Brijesh Kumr Ri Deprtment of Electronics nd Electricl Engineering Indin Institute of Technology Guwhti, Guwhti, Assm, Indi Emil: {d.nildri, bkri}@iitg.ernet.in
More informationDOI:.8/nc5 Cpilities of MCAK Sidesliding, endctching on microtuules MCAKdecorted ed Functions in mitotic spindle Prometphse Slides on the microtuule surfce + Redily slides long the microtuule surfce Strongly
More informationTests for the Ratio of Two Poisson Rates
Chpter 437 Tests for the Rtio of Two Poisson Rtes Introduction The Poisson probbility lw gives the probbility distribution of the number of events occurring in specified intervl of time or spce. The Poisson
More informationSupplementary Information for Directional Reflective Surface Formed via Gradient- Impeding Acoustic Meta-surfaces
Supplementry Informtion for Directionl Reflective Surfce Formed vi Grdient- Impeding Acoustic Met-surfces Kyungjun Song 1*, Jedo Kim 2, Hur Shin 1, Jun-Hyuk Kwk 1, Seong-Hyun Lee 3,Tesung Kim 4 1 Deprtment
More information1 Online Learning and Regret Minimization
2.997 Decision-Mking in Lrge-Scle Systems My 10 MIT, Spring 2004 Hndout #29 Lecture Note 24 1 Online Lerning nd Regret Minimiztion In this lecture, we consider the problem of sequentil decision mking in
More informationState space systems analysis (continued) Stability. A. Definitions A system is said to be Asymptotically Stable (AS) when it satisfies
Stte spce systems nlysis (continued) Stbility A. Definitions A system is sid to be Asymptoticlly Stble (AS) when it stisfies ut () = 0, t > 0 lim xt () 0. t A system is AS if nd only if the impulse response
More informationftp.fe?a:fmmmhm Quickly get policy ) long generally equilibrium independent steady P # steady E amp= : Dog steady systems Every equilibrium by density
Introduction fle SI 3 61 Non equilibrium Sttisticl Mechnics 2520 1 Techer : Tens It Brdrsonbrdrson@kthse Office 174 : 1049 ( open door policy Lecture 1 & The second lw nd origin of irreversibility Wht
More informationFirst Semester Review Calculus BC
First Semester Review lculus. Wht is the coordinte of the point of inflection on the grph of Multiple hoice: No lcultor y 3 3 5 4? 5 0 0 3 5 0. The grph of piecewise-liner function f, for 4, is shown below.
More informationMATH SS124 Sec 39 Concepts summary with examples
This note is mde for students in MTH124 Section 39 to review most(not ll) topics I think we covered in this semester, nd there s exmples fter these concepts, go over this note nd try to solve those exmples
More informationZ0103/07/09 series. Blocking voltage to 800 V (NA and NN types)
Rev. September Product dt. Product profile. Description Pssivted trics in conventionl nd surfce mounting pckges. Intended for use in pplictions requiring high bidirectionl trnsient nd blocking voltge cpbility.
More informationThermodynamic description of Tc(IV) solubility and hydrolysis in dilute to concentrated NaCl, MgCl 2 and CaCl 2 solutions
Electronic Supplementry Mteril (ESI for Dlton Trnsctions. This journl is The Royl Society of Chemistry 2016 Thermodynmic description of Tc(IV solubility nd hydrolysis in dilute to concentrted NCl, MgCl
More informationSupplementary Material for Wave-pinning and cell polarity from a bistable reaction-diffusion system
Supplementry Mteril for Wve-pinning nd cell polrity from bistble rection-diffusion system Yoichiro Mori, Alendr Jilkine nd Leh Edelstein-Keshet Model Comprisons We concentrte here the three systems to
More information1 Module for Year 10 Secondary School Student Logarithm
1 Erthquke Intensity Mesurement (The Richter Scle) Dr Chrles Richter showed tht the lrger the energy of n erthquke hs, the lrger mplitude of ground motion t given distnce. The simple model of Richter
More informationa cacnb1 ts25/ts25 Supplemental Figure 1
ccn1 ts/ts α -ungrotoxin prlyzed 0.6 ΔF/F 0.0 2 ΔF/F 2 s stimulus α -ungrotoxin ccn1 ts/ts Supplementl Figure 1 CSF-cNs recorded from lrv prlyzed with α-ungrotoxin nd ccn1 mutnt lrv show no difference
More informationLecture 6: Diffusion and Reaction kinetics
Lecture 6: Diffusion nd Rection kinetics 1-1-1 Lecture pln: diffusion thermodynmic forces evolution of concentrtion distribution rection rtes nd methods to determine them rection mechnism in terms of the
More informationProbability-Based Seismic Assessments: Implementing Wide-Range Nonlinear Dynamic Analysis Methods
Probbility-Bsed Seismic Assessments: Implementing Nonliner Dynmic Anlysis Methods Ftim Jlyer Postdoctorl Scholr University of Rome L Spienz Cliforni Institute of Technology (CIT) Outline A brief Introduction
More informationFig. 1. Open-Loop and Closed-Loop Systems with Plant Variations
ME 3600 Control ystems Chrcteristics of Open-Loop nd Closed-Loop ystems Importnt Control ystem Chrcteristics o ensitivity of system response to prmetric vritions cn be reduced o rnsient nd stedy-stte responses
More informationPopulation bottleneck : dramatic reduction of population size followed by rapid expansion,
Selection We hve defined nucleotide diversity denoted by π s the proportion of nucleotides tht differ between two rndomly chosen sequences. We hve shown tht E[π] = θ = 4 e µ where µ cn be estimted directly.
More informationHigher Checklist (Unit 3) Higher Checklist (Unit 3) Vectors
Vectors Skill Achieved? Know tht sclr is quntity tht hs only size (no direction) Identify rel-life exmples of sclrs such s, temperture, mss, distnce, time, speed, energy nd electric chrge Know tht vector
More informationSUPPLEMENTARY INFORMATION
doi:.38/nture896 c Resting stte Resting stte Resting stte Supplementry Figure Illustrtion of pttern clssifiction nd its effects on neuronl representtions. Dynmic ctivity ptterns evoked y different stimuli
More informationUNIT 1 FUNCTIONS AND THEIR INVERSES Lesson 1.4: Logarithmic Functions as Inverses Instruction
Lesson : Logrithmic Functions s Inverses Prerequisite Skills This lesson requires the use of the following skills: determining the dependent nd independent vribles in n exponentil function bsed on dt from
More informationAcceptance Sampling by Attributes
Introduction Acceptnce Smpling by Attributes Acceptnce smpling is concerned with inspection nd decision mking regrding products. Three spects of smpling re importnt: o Involves rndom smpling of n entire
More informationComparison Procedures
Comprison Procedures Single Fctor, Between-Subects Cse /8/ Comprison Procedures, One-Fctor ANOVA, Between Subects Two Comprison Strtegies post hoc (fter-the-fct) pproch You re interested in discovering
More information( dg. ) 2 dt. + dt. dt j + dh. + dt. r(t) dt. Comparing this equation with the one listed above for the length of see that
Arc Length of Curves in Three Dimensionl Spce If the vector function r(t) f(t) i + g(t) j + h(t) k trces out the curve C s t vries, we cn mesure distnces long C using formul nerly identicl to one tht we
More informationDriving Cycle Construction of City Road for Hybrid Bus Based on Markov Process Deng Pan1, a, Fengchun Sun1,b*, Hongwen He1, c, Jiankun Peng1, d
Interntionl Industril Informtics nd Computer Engineering Conference (IIICEC 15) Driving Cycle Construction of City Rod for Hybrid Bus Bsed on Mrkov Process Deng Pn1,, Fengchun Sun1,b*, Hongwen He1, c,
More informationNew data structures to reduce data size and search time
New dt structures to reduce dt size nd serch time Tsuneo Kuwbr Deprtment of Informtion Sciences, Fculty of Science, Kngw University, Hirtsuk-shi, Jpn FIT2018 1D-1, No2, pp1-4 Copyright (c)2018 by The Institute
More informationDo the one-dimensional kinetic energy and momentum operators commute? If not, what operator does their commutator represent?
1 Problem 1 Do the one-dimensionl kinetic energy nd momentum opertors commute? If not, wht opertor does their commuttor represent? KE ˆ h m d ˆP i h d 1.1 Solution This question requires clculting the
More informationP 3 (x) = f(0) + f (0)x + f (0) 2. x 2 + f (0) . In the problem set, you are asked to show, in general, the n th order term is a n = f (n) (0)
1 Tylor polynomils In Section 3.5, we discussed how to pproximte function f(x) round point in terms of its first derivtive f (x) evluted t, tht is using the liner pproximtion f() + f ()(x ). We clled this
More informationDiverse modes of eco-evolutionary dynamics in communities of antibiotic-producing microorganisms
In the formt provided y the uthors nd unedited. ULEMENTAY INFOMATION VOLUME: 1 ATICLE NUMBE: 0189 iverse modes of eco-evolutionry dynmics in communities of ntiiotic-producing microorgnisms Klin Vetsigin
More informationDesigning Information Devices and Systems I Discussion 8B
Lst Updted: 2018-10-17 19:40 1 EECS 16A Fll 2018 Designing Informtion Devices nd Systems I Discussion 8B 1. Why Bother With Thévenin Anywy? () Find Thévenin eqiuvlent for the circuit shown elow. 2kΩ 5V
More informationDirect indirect character of the band gap in methylammonium lead iodide perovskite
Direct indirect chrcter of the nd gp in methylmmonium led iodide perovskite Eline M. Hutter 1, Mrí C. Gélvez-Rued 1, Ann Osherov 2, Vldimir Bulović 2, Ferdinnd C. Grozem 1, Smuel D. Strnks 2,3*, nd Tom
More informationSupplementary Figure 1 Supplementary Figure 2
Supplementry Figure 1 Comprtive illustrtion of the steps required to decorte n oxide support AO with ctlyst prticles M through chemicl infiltrtion or in situ redox exsolution. () chemicl infiltrtion usully
More informationPredict Global Earth Temperature using Linier Regression
Predict Globl Erth Temperture using Linier Regression Edwin Swndi Sijbt (23516012) Progrm Studi Mgister Informtik Sekolh Teknik Elektro dn Informtik ITB Jl. Gnesh 10 Bndung 40132, Indonesi 23516012@std.stei.itb.c.id
More informationVorticity. curvature: shear: fluid elements moving in a straight line but at different speeds. t 1 t 2. ATM60, Shu-Hua Chen
Vorticity We hve previously discussed the ngulr velocity s mesure of rottion of body. This is suitble quntity for body tht retins its shpe but fluid cn distort nd we must consider two components to rottion:
More informationANALYSIS OF FAST REACTORS SYSTEMS
ANALYSIS OF FAST REACTORS SYSTEMS M. Rghe 4/7/006 INTRODUCTION Fst rectors differ from therml rectors in severl spects nd require specil tretment. The prsitic cpture cross sections in the fuel, coolnt
More informationChapter 5 : Continuous Random Variables
STAT/MATH 395 A - PROBABILITY II UW Winter Qurter 216 Néhémy Lim Chpter 5 : Continuous Rndom Vribles Nottions. N {, 1, 2,...}, set of nturl numbers (i.e. ll nonnegtive integers); N {1, 2,...}, set of ll
More informationTerminal Velocity and Raindrop Growth
Terminl Velocity nd Rindrop Growth Terminl velocity for rindrop represents blnce in which weight mss times grvity is equl to drg force. F 3 π3 ρ L g in which is drop rdius, g is grvittionl ccelertion,
More informationMinimum Energy State of Plasmas with an Internal Transport Barrier
Minimum Energy Stte of Plsms with n Internl Trnsport Brrier T. Tmno ), I. Ktnum ), Y. Skmoto ) ) Formerly, Plsm Reserch Center, University of Tsukub, Tsukub, Ibrki, Jpn ) Plsm Reserch Center, University
More informationFunctional Role of Loop 2 in Myosin V
Biochemistry 2004, 43, 2605-2612 2605 Functionl Role of Loop 2 in Myosin V Christopher M. Yengo nd H. Lee Sweeney* Deprtment of Physiology, UniVersity of PennsylVni School of Medicine, Phildelphi, PennsylVni
More informationThermal Diffusivity. Paul Hughes. Department of Physics and Astronomy The University of Manchester Manchester M13 9PL. Second Year Laboratory Report
Therml iffusivity Pul Hughes eprtment of Physics nd Astronomy The University of nchester nchester 3 9PL Second Yer Lbortory Report Nov 4 Abstrct We investigted the therml diffusivity of cylindricl block
More informationAlg. Sheet (1) Department : Math Form : 3 rd prep. Sheet
Ciro Governorte Nozh Directorte of Eduction Nozh Lnguge Schools Ismili Rod Deprtment : Mth Form : rd prep. Sheet Alg. Sheet () [] Find the vlues of nd in ech of the following if : ) (, ) ( -5, 9 ) ) (,
More information6. Photoionization of acridine through singlet and triplet channels
Chpter 6: Photoioniztion of cridine through singlet nd triplet chnnels 59 6. Photoioniztion of cridine through singlet nd triplet chnnels Photoioinztion of cridine (Ac) in queous micelles hs not yet een
More informationRole of Missing Carotenoid in Reducing the Fluorescence of Single Monomeric Photosystem II Core Complexes
Electronic Supplementry Mteril (ESI for Physicl Chemistry Chemicl Physics. This journl is the Owner Societies 017 Supporting Informtion Role of Missing Crotenoid in Reducing the Fluorescence of Single
More informationTremor-rich shallow dyke formation followed by silent magma flow at Bárðarbunga in Iceland
In the formt provided y the uthors nd unedited. SUPPLEMENTARY INFORMATION DOI: 1.138/NGEO9 Tremor-rich shllow dyke formtion followed y silent mgm flow t Bárðrung in Icelnd 1,, 1, 3 1, 1 1, NATURE GEOSCIENCE
More informationShear Degradation and Possible viscoelastic properties of High Molecular Weight Oil Drag Reducer Polymers
ANNUAL TRANSACTIONS OF THE NORDIC RHEOLOGY SOCIETY, VOL. 3, 2005 Sher Degrdtion nd Possible viscoelstic properties of High Moleculr Weight Oil Drg Reducer Polymers A.A. Hmoud, C. Elissen, C. Idsøe nd T.
More informationIntroduction to Electronic Circuits. DC Circuit Analysis: Transient Response of RC Circuits
Introduction to Electronic ircuits D ircuit Anlysis: Trnsient esponse of ircuits Up until this point, we hve een looking t the Stedy Stte response of D circuits. StedyStte implies tht nothing hs chnged
More informationROB EBY Blinn College Mathematics Department
ROB EBY Blinn College Mthemtics Deprtment Mthemtics Deprtment 5.1, 5.2 Are, Definite Integrls MATH 2413 Rob Eby-Fll 26 Weknowthtwhengiventhedistncefunction, wecnfindthevelocitytnypointbyfindingthederivtiveorinstntneous
More informationVerification Analysis of the Slope Stability
Verifiction nul no. 3 Updte 04/016 Verifiction Anlysis of the Slope Stbility Progr: File: Slope Stbility Deo_v_en_03.gst In this verifiction nul you will find hnd-de verifiction nlysis of the stbility
More informationNonlocal Gravity and Structure in the Universe
Nonlocl rvity nd Structure in the Universe Sohyun Prk Penn Stte University Co-uthor: Scott Dodelson Bsed on PRD 87 (013) 04003, 109.0836, PRD 90 (014) 000000, 1310.439 August 5, 014 Chicgo, IL Cosmo 014
More informationRecitation 3: More Applications of the Derivative
Mth 1c TA: Pdric Brtlett Recittion 3: More Applictions of the Derivtive Week 3 Cltech 2012 1 Rndom Question Question 1 A grph consists of the following: A set V of vertices. A set E of edges where ech
More informationStudent Activity 3: Single Factor ANOVA
MATH 40 Student Activity 3: Single Fctor ANOVA Some Bsic Concepts In designed experiment, two or more tretments, or combintions of tretments, is pplied to experimentl units The number of tretments, whether
More information1 Error Analysis of Simple Rules for Numerical Integration
cs41: introduction to numericl nlysis 11/16/10 Lecture 19: Numericl Integrtion II Instructor: Professor Amos Ron Scries: Mrk Cowlishw, Nthnel Fillmore 1 Error Anlysis of Simple Rules for Numericl Integrtion
More informationPopulation Dynamics Definition Model A model is defined as a physical representation of any natural phenomena Example: 1. A miniature building model.
Popultion Dynmics Definition Model A model is defined s physicl representtion of ny nturl phenomen Exmple: 1. A miniture building model. 2. A children cycle prk depicting the trffic signls 3. Disply of
More informationJack Simons, Henry Eyring Scientist and Professor Chemistry Department University of Utah
1. Born-Oppenheimer pprox.- energy surfces 2. Men-field (Hrtree-Fock) theory- orbitls 3. Pros nd cons of HF- RHF, UHF 4. Beyond HF- why? 5. First, one usully does HF-how? 6. Bsis sets nd nottions 7. MPn,
More informationChem/Biochem 471 Exam 2 11/18/09 Page 1 of 7 Name:
Chem/Biochem 471 Exm 2 11/18/09 Pge 1 of 7 Plese leve the exm pges stpled together. The formuls re on seprte sheet. This exm hs 5 questions. You must nswer t lest 4 of the questions. You my nswer more
More informationSupplementary Information
Supplementry Informtion Spontneously mplified homochirl orgnic-inorgnic nno-helix complex vi self-prolifertion Hlei Zhi, Yn Qun, Li Li, Xing-Yng Liu, Xurong Xu c nd Ruikng Tng*,c Centre for Biomterils
More informationL35-Wed-23-Nov-2016-Sec-5-5-Properties-of-Logs-HW36-Moodle-Q29
L35-Wed-3-Nov-016-Sec-5-5-Properties-of-Logs-HW36-Moodle-Q9 pge 49 L35-Wed-3-Nov-016-Sec-5-5-Properties-of-Logs-HW36-Moodle-Q9 We hve looked t severl chrcteristics of the log function. Now, we will look
More informationStructural Effect of Thioureas on the Detection of Chemical Warfare Agent Simulants
Structurl Effect of Thioures on the Detection of Chemicl Wrfre Agent Simulnts Seonggyun H, Minhe Lee, Hyun Ook Seo, b Sun Gu Song, Kyung-su Kim, Chn Heum Prk, Il Hee Kim, Young Dok Kim nd Chngsik Song*
More informationA Brief Review on Akkar, Sandikkaya and Bommer (ASB13) GMPE
Southwestern U.S. Ground Motion Chrcteriztion Senior Seismic Hzrd Anlysis Committee Level 3 Workshop #2 October 22-24, 2013 A Brief Review on Akkr, Sndikky nd Bommer (ASB13 GMPE Sinn Akkr Deprtment of
More informationHow do we solve these things, especially when they get complicated? How do we know when a system has a solution, and when is it unique?
XII. LINEAR ALGEBRA: SOLVING SYSTEMS OF EQUATIONS Tody we re going to tlk bout solving systems of liner equtions. These re problems tht give couple of equtions with couple of unknowns, like: 6 2 3 7 4
More informationGenetic Programming. Outline. Evolutionary Strategies. Evolutionary strategies Genetic programming Summary
Outline Genetic Progrmming Evolutionry strtegies Genetic progrmming Summry Bsed on the mteril provided y Professor Michel Negnevitsky Evolutionry Strtegies An pproch simulting nturl evolution ws proposed
More informationNUMERICAL INTEGRATION. The inverse process to differentiation in calculus is integration. Mathematically, integration is represented by.
NUMERICAL INTEGRATION 1 Introduction The inverse process to differentition in clculus is integrtion. Mthemticlly, integrtion is represented by f(x) dx which stnds for the integrl of the function f(x) with
More informationHow do we solve these things, especially when they get complicated? How do we know when a system has a solution, and when is it unique?
XII. LINEAR ALGEBRA: SOLVING SYSTEMS OF EQUATIONS Tody we re going to tlk out solving systems of liner equtions. These re prolems tht give couple of equtions with couple of unknowns, like: 6= x + x 7=
More informationDensity of Energy Stored in the Electric Field
Density of Energy Stored in the Electric Field Deprtment of Physics, Cornell University c Tomás A. Aris October 14, 01 Figure 1: Digrm of Crtesin vortices from René Descrtes Principi philosophie, published
More informationDERIVATIVES NOTES HARRIS MATH CAMP Introduction
f DERIVATIVES NOTES HARRIS MATH CAMP 208. Introduction Reding: Section 2. The derivtive of function t point is the slope of the tngent line to the function t tht point. Wht does this men, nd how do we
More informationW. We shall do so one by one, starting with I 1, and we shall do it greedily, trying
Vitli covers 1 Definition. A Vitli cover of set E R is set V of closed intervls with positive length so tht, for every δ > 0 nd every x E, there is some I V with λ(i ) < δ nd x I. 2 Lemm (Vitli covering)
More informationCalculus in R. Chapter Di erentiation
Chpter 3 Clculus in R 3.1 Di erentition Definition 3.1. Suppose U R is open. A function f : U! R is di erentible t x 2 U if there exists number m such tht lim y!0 pple f(x + y) f(x) my y =0. If f is di
More informationpolyimide Spray-coated ZrP/epoxy film Spray-coated ZrP/epoxy film glass
c d e polyimide Spry-coted ZrP/epoxy film glss Spry-coted ZrP/epoxy film f g Supplementry Figure 1. Opticl microscopy of smectic ( = 0.044) α-zrp/epoxy films., Trnsmission opticl microscopy (TOM) of smectic
More information4- Cosmology - II. introduc)on to Astrophysics, C. Bertulani, Texas A&M-Commerce 1
4- Cosmology - II introduc)on to Astrophysics, C. Bertulni, Texs A&M-Commerce 1 4.1 - Solutions of Friedmnn Eqution As shown in Lecture 3, Friedmnn eqution is given by! H 2 = # " & % 2 = 8πG 3 ρ k 2 +
More informationDiscrete Mathematics and Probability Theory Spring 2013 Anant Sahai Lecture 17
EECS 70 Discrete Mthemtics nd Proility Theory Spring 2013 Annt Shi Lecture 17 I.I.D. Rndom Vriles Estimting the is of coin Question: We wnt to estimte the proportion p of Democrts in the US popultion,
More information15. Quantisation Noise and Nonuniform Quantisation
5. Quntistion Noise nd Nonuniform Quntistion In PCM, n nlogue signl is smpled, quntised, nd coded into sequence of digits. Once we hve quntised the smpled signls, the exct vlues of the smpled signls cn
More informationPartial Derivatives. Limits. For a single variable function f (x), the limit lim
Limits Prtil Derivtives For single vrible function f (x), the limit lim x f (x) exists only if the right-hnd side limit equls to the left-hnd side limit, i.e., lim f (x) = lim f (x). x x + For two vribles
More informationIN THE EXTENDED NAMBU JONA-LASINIO MODEL
THE DECAYS ( (89), (4), (7), (65), (6), (64)) IN THE EXTENDED NAMBU JONA-LASINIO MODEL M.. Volkov,. Nurln, Bogoliubov Lbortory of Theoreticl Physics, JINR, Dubn 498, Russi. Dubn Stte University, Dubn 498,
More informationTransient Stimulation of Distinct Subpopulations of Striatal Neurons Mimics Changes in the Value of Competing Actions
Supplementl mterils for: Trnsient Stimultion of Distinct Supopultions of Stritl Neurons Mimics Chnges in the Vlue of Competing Actions Lung-Ho Ti, A. Moses Lee,2, Nor Benvidez 3, Antonello Bonci 4,,6,
More informationOperations with Polynomials
38 Chpter P Prerequisites P.4 Opertions with Polynomils Wht you should lern: How to identify the leding coefficients nd degrees of polynomils How to dd nd subtrct polynomils How to multiply polynomils
More informationWMAP satellite. 16 Feb Feb Feb 2012
16 Feb 2012 21 Feb 2012 23 Feb 2012 è Announcements è Problem 5 (Hrtle 18.3). Assume V * is nonreltivistic. The reltivistic cse requires more complicted functions. è Outline è WMAP stellite è Dipole nisotropy
More information1 Part II: Numerical Integration
Mth 4 Lb 1 Prt II: Numericl Integrtion This section includes severl techniques for getting pproimte numericl vlues for definite integrls without using ntiderivtives. Mthemticll, ect nswers re preferble
More informationSUPPLEMENTARY INFORMATION
DOI: 10.1038/NCHEM.2398 Locl virtionl coherences drive the primry photochemistry of vision Philip J. M. Johnson 1, Alexei Hlpin 1, Tkefumi Morizumi 2, Vlentyn I. Prokhorenko 3, Oliver P. Ernst 2,4, & R.
More information