GENETICS I: Introduction to Genetics. Assist. Prof. Dr. Betul Akcesme
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1 GENETICS I: Introduction to Genetics Assist. Prof. Dr. Betul Akcesme
2 2 NS209 Genetics I Office: F1.7 bakcesme@ius.edu.ba Monday-Wednesday 13:30-14:45 Classroom: A F1. 10
3 Books: Genetics From Genes to genomes. Hartwell. Hood. Goldberg. Reynolds. Silver. Veres. 4 th edition Essential of Genetics, by W. Klug, M. Cummings, C. Spencer, M. Palladino, 9 th edition,
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5 Weekly schedule
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7 Summarized content of the course Week 1 Introduction to Genetics DNA structure Week 2 Chromosome and Chromatin structure
8 8 Week 3-4 Mitosis Meiosis
9 9 Week 5-6 Mendelian Genetics Law of segregation Law of independent assortment
10 10 Week 7 Extensions to Mendel's Law Week 8 Gene structure and organization overview
11 11 Week 9 Replication and recombination Week 10 Mutations
12 12 Week 11 Gene Expression: The Flow of Information from DNA to RNA Week 12 The Flow of Information from RNA to Protein: TRANSLATION
13 13 Week 13 Linkage, Recombination, and the mapping of genes on chromosome Week 14 Digital Analysis of DNA PCR
14 Lab Activities and tutorials: Weekly basis tutorials for practice! Lab activities will be announced before one week. 1. Mitosis 2.Meiosis 3. PCR Lab reports!
15 SEVERAL REMINDERS!! Attendance of lectures and tutorials are MANDATORY! Up to 30% absence is tolerated! ( 9 out of 28) Submission of assignments and lab reports on time! Copy-Past is strictly forbidden for assignments and lab reports!!
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17 What is Genetics? the study of heredity and the variation of inherited characteristics. Why studying genetics? Why is genetics important? What are the reasons of its rapid development?
18 What is Genetics? Explains how are the traits inherited from parents to offspring. The field of natural sciences concerned with the diversity, replication, mutation and expression of the information in the genes.
19 19 The importance of genetics Genes influence our lives! How? They affect our: Height Weight Hair color Skin pigmentation Our susceptibility to diseases Contribute to our inteligence and personality
20 20 Some traits determined by our genes Dominant Low heart rate Unattached (free) earlobe straight nose extra finger or toe Curly Hair A and B blood type Broad Lips large eyes Darker hair long eyelashes Slower aging Recessive High heart rate Attached earlobe turned up nose Normal 5 fingers and toes Flat hair O blood type Slender lips Small eyes Lighter hair Short eyelashes accelerated aging
21 21 Genes are fundamental to WHO and WHAT we are Agriculture Genetics influenced: Pharmaceutical industry Biotechnology Medicine
22 22 The role of genetics in biology Understanding of genetics is important to ALL people, but CRUCIAL to the students in the life sciences.
23 23 Genetics provides one of the biology s unifying principles: all organisms - Use the same genetic system The study of all most every field of biology is incomplete without understanding of genes (and genetic methods) Genetic variation is the foundation of the diversity of all life
24 24 Basic division of Genetics Transmission genetics (Mendelian Genetics) Population genetics Molecular genetics Quantitative genetics
25 25 Transmission genetics -Mendelian Genetics FOCUS: is on INDIVIDUAL How an individual organism inherits its genetic make up and how it passes its genes to the next generation Phenotype Cell and chromosomes Cell division Simple and complicated forms of inheritance
26 26 Molecular Genetics FOCUS: is the GENE Its structure, organization and function
27 27 Population genetics FOCUS: the group of genes found in a POPULATION it s a search for patterns that help describe the genetic signature of a particular group
28 Quantitative Genetics A highly mathematical field that examines the statistical relationships between genes and the traits they encode.
29 29 Model Organisms Almost all major groups of Bacteria Fungi Protists Plants and Animals Model organisms: organisms with characteristics that make them particularly useful for genetic analysis About which a large amount of genetic information has been accumulated
30 30 Classical genetics 1865: Gregor Mendel's paper, Experiments on Plant Hybridization 1869: Friedrich Miescher discovers a weak acid in the nuclei of white blood cells that today we call DNA 1889: Hugo de Vries postulates that "inheritance of specific traits in organisms comes in particles", naming such particles "(pan)genes" 1903: Walter Sutton and Theodor Boveri hypothesizes that chromosomes, which segregate in a Mendelian fashion, are hereditary units 1908: Hardy-Weinberg law derived 1910: Thomas Hunt Morgan shows that genes reside on chromosomes 1913: Alfred Sturtevant makes the first genetic map of a chromosome 1928: Frederick Griffith discovers that hereditary material from dead bacteria can be incorporated into live bacteria (see Griffith's experiment) 1931: Crossing over is identified as the cause of recombination 1941: Edward Lawrie Tatum and George Wells Beadle show that genes code for proteins; see the original central dogma of genetics
31 : The Avery MacLeod McCarty experiment isolates DNA as the genetic material (at that time called transforming principle) 1948: Barbara McClintock discovers transposons in maize The DNA era 1950: Erwin Chargaff shows that the four nucleotides are not present in nucleic acids in stable proportions, but that some general rules appear to hold (e.g., that the amount of adenine, A, tends to be equal to that of thymine, T). 1952: The Hershey-Chase experiment proves the genetic information of phages (and all other organisms) to be DNA 1953: DNA structure is resolved to be a double helix by James D. Watson and Francis Crick [11] 1956: Joe Hin Tjio and Albert Levan established the correct chromosome number in humans to be : The Meselson-Stahl experiment demonstrates that DNA is semiconservatively replicated : Combined efforts of scientists "crack" the genetic code, including Marshall Nirenberg, Har Gobind Khorana, Sydney Brenner & Francis Crick 1964: Howard Temin showed using RNA viruses that the direction of DNA to RNA transcription can be reversed 1970: Restriction enzymes were discovered in studies of a bacterium, Haemophilus influenzae, enabling scientists to cut and paste DNA
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33 The genomics era 1972: Walter Fiers and his team at the Laboratory of Molecular Biology of the University of Ghent (Ghent, Belgium) were the first to determine the sequence of a gene: the gene for bacteriophage MS2 coat protein : DNA is sequenced for the first time by Fred Sanger, Walter Gilbert, and Allan Maxam working independently. Sanger's lab sequence the entire genome of bacteriophage Φ-X : Kary Banks Mullis discovers the polymerase chain reaction enabling the easy amplification of DNA 1989: The human gene that encodes the CFTR protein was sequenced by Francis Collins and Lap-Chee Tsui. Defects in this gene cause cystic fibrosis 1995: The genome of Haemophilus influenzae is the first genome of a free living organism to be sequenced 1996: Saccharomyces cerevisiae is the first eukaryote genome sequence to be released 1998: The first genome sequence for a multicellular eukaryote, Caenorhabditis elegans, is released 2001: First draft sequences of the human genome are released simultaneously by the Human Genome Project and Celera Genomics (April 14 th ) : Successful completion of Human Genome Project with 99% of the genome sequenced to a 99.99% accuracy
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