ept.i.p.s. Version A
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1 ept.i.p.s. Fig. Tab. p. Instructions see p. for for use use ept.i.p.s. Instructions for use Version A
2 Copyright 2012 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior permission of the copyright owner. Biopur, Eppendorf, Eppendorf Research, Eppendorf Reference, Eppendorf Xplorer, ept.i.p.s. and ep Dualfilter T.I.P.S. are registered trademarks of Eppendorf AG, Hamburg, Germany. Registered trademarks are not marked in all cases with or in this manual / Version A
3 Table of contents 1 Table of contents 1 Introduction Instructions for use Intended use Operation Handling reloads ep Dualfilter T.I.P.S ept.i.p.s Autoclave pipet tips Purity grades and certificates Eppendorf purity grades Certificates - verifiable safety for you Troubleshooting General errors ept.i.p.s. combinations Technical data Ambient conditions Transport, storage and disposal Storage
4 1 Introduction 1 Introduction ept.i.p.s. pipette tips are disposable plastic tips made of polypropylene (PP). ept.i.p.s. stands for Eppendorf Totally Integrated Pipetting System which means that form, material properties and fit are optimized for Eppendorf s manual and electronic piston-stroke pipettes. The tips are available in a variety of finishes and purity grades. Every tray for ept.i.p.s. pipette tips is color-coded and corresponds to the color of the control buttons of Eppendorf pipettes. Beside ept.i.p.s. pipette tips we offer ep Dualfilter T.I.P.S. pipette tips to minimize the risk of contamination, ept.i.p.s. pipette tips to dispense liquids containing detergents and ep Dualfilter T.I.P.S. pipette tips which combines both. 2 Instructions for use The pipette tips ept.i.p.s., ep Dualfilter T.I.P.S., ept.i.p.s. and ep Dualfilter T.I.P.S. and relevant pipettes must be used by trained laboratory workers. Before using tips familiarize yourself with the operating manual of the relevant instrument. Before using the tips, check pack and contents carefully for any damage. For filter tips, check whether there is a dualfilter in every tip and whether it is correctly positioned, undamaged and not twisted. If you detect any fault, do not use the tip. The tips should always be emptied into a container so that no fluid sprays into the environment. After use, dispose of tips in accordance with the relevant legal regulations. Tips should not be filled with fluids which attack PP, or in the case of filter tips also PE. If you are in any doubt as to the suitability of your product for its intended use or as to the reagent, please contact our hotline. You will find phone number and address on the last page. 2 Instructions for use 3 Intended use 3 Intended use The pipette tips ept.i.p.s. of the packaging units Reloads, Box, Set, Singles, and Racks of the sizes 0.1 μl 10 μl, 0.1 μl 20 μl, 0.5 μl 20 μl L, 2 μl 200 μl, 20 μl 300 μl, 50 μl μl, in combination with the corresponding pipettes, are designed for the transmission of liquids with low contamination, especially to make it possible to use the in vitro diagnostics according to their intended use for samples from the human body and/or for reagents in the context of an in vitro diagnostic application. Therefore, the above-mentioned pipette tips as accessories for in vitro diagnostics are themselves in vitro diagnostics in terms of the guideline 98/79/EG of the European Parliament and Council of October 27th, 1998, about in vitro diagnostics. The pipette tips only are intended for application by trained specialists and only for single use. 4
5 4 Operation 4 Operation You will find recommendations for handling in the operating manual for the instrument you are using. The dispensing techniques used most frequently are forward pipetting, reverse pipetting, dispensing, sequential dispensing and diluting. Mechanical pipettes are only suitable for forward and reverse pipetting. Irrespective of the dispensing technique use, the following items should be taken into consideration during pipetting: When aspirating the liquid, the tip should only be immersed a few millimeters into the medium. The filled tip should be moved up against the wall of the vessel to avoid residues of liquid on the outside of the tip. Pre-wetting the tip up to three times will improve the accuracy and precision of the results. Liquid should be aspirated slowly and evenly. A waiting period of 3 seconds should be allowed for the liquid to rise in the tip. The pipette should be held vertically during aspiration. 5
6 ept.i.p.s. Instructions for use 5 5 Handling reloads Handling reloads "c " lic 6
7 6 ep Dualfilter T.I.P.S. 6 ep Dualfilter T.I.P.S. During pipetting liquid flows through a small opening. A possible outcome of this are aerosols (dispersion of solid or liquid particles in gas). Aerosols, drops and splashes could contaminate the pipette and contaminations could be transferred to other samples. To avoid these unintended and unnoticed contaminations ep Dualfilter T.I.P.S. pipette tips should be used. The dualfilter is made up of hydrophobic polyethylene (PE). The first white layer defends from splashes and drops and the second layer builds an aerosol barrier. ep Dualfilter T.I.P.S. pipette tips are sterilized by electron beams and are certified (see Certificates - verifiable safety for you on p. 9). ep Dualfilter T.I.P.S. pipette tips can not be sterilized. 7 ept.i.p.s. 7 ept.i.p.s. ept.i.p.s. pipette tips are particularly suited to the dispensing of liquids containing detergents. The optimized surface of the ept.i.p.s. pipette tips counteracts the reduced surface tension caused by detergents and also with corresponding solvents, so that the liquid runs off without forming a film. This increases sample recovery and pipetting accuracy. Please note: The small amounts of residual moisture that may occur with ept.i.p.s. pipette tips can appear to be higher than with standard ept.i.p.s. pipette tips as the residual moisture does not remain in the tip as a film, but collects as droplets due to the physical properties. Due to the physical properties of the surface of ept.i.p.s. pipette tips, the dispensing volume is slightly lower than the nominal volume in comparison to the corresponding standard ept.i.p.s. pipette tips when dispensing purely aqueous solutions with ept.i.p.s. pipette tips μl S and μl L. Reproducibility still has the same high performance as standard ept.i.p.s. pipette tips. 8 Autoclave pipet tips 8 Autoclave pipet tips Hint! Don not use any additional desinfectants during autoclaving for box and rack. Product Temperature Pressure Time Number of possible cycles ep T.I.P.S. and ep T.I.P.S. 121 C 1 bar 20 min 5 times ep T.I.P.S. Rack 121 C 1 bar 20 min 10 times ep T.I.P.S. Box 121 C 1 bar 20 min 100 times ep Dualfilter T.I.P.S. and ep Can not be autoclaved. Dualfilter T.I.P.S. 1. Remove the packaging foil. 2. Autoclave the pipet tips. 7
8 9 Purity grades and certificates 9 Purity grades and certificates 9.1 Eppendorf purity grades Sterile + PCR clean Eppendorf Quality Sterile PCR clean Biopur Relevant quality criteria Function, tightness, precision Low wettability High chemical resistance High thermal stability High transparency Precisely molded Batch testing (certified) for the following purity criteria Human DNA-free DNA-free (free of human + bacterial DNA) DNase-free RNase-free PCR inhibitor-free ATP-free Pyrogen-free (endotoxin-free) 8
9 Sterile + PCR clean Eppendorf Quality Sterile PCR clean Biopur Sterile (European Pharmacopoeia/USP) ept.i.p.s. ept.i.p.s. Standard + Box + Set ept.i.p.s. Reloads ept.i.p.s. Racks ept.i.p.s. Singles ep Dualfilter T.I.P.S. ept.i.p.s. ep Dualfilter T.I.P.S. 9.2 Certificates - verifiable safety for you The number of standards, guidelines and specifications that apply to biological, diagnostic and industrial laboratories is constantly on the rise. As a result, the need to provide proof of observing these requirements has also increased. Three certificate categories are available for Eppendorf liquid handling consumables: 1. Batch-specific certificates are prepared by externally commissioned and recognized laboratories for the following purity grades: Biopur PCR clean PCR clean and Sterile You can access the batch-specific certificate at certificates after entering the batch number of your product. 9
10 2. General certificates of conformity such as: Certificate of conformity for products in the PCR clean purity grade Certificate of conformity for verifying metal trace concentrations Certificate of conformity on the absence of surface active additives Certificate of conformity for HEPA classification of ep Dualfilter T.I.P.S. filter tips 3. Quality management and ISO certificates which verify conformity to ISO All certificates can be downloaded from our homepage 10 Troubleshooting 10 Troubleshooting 10.1 General errors Symptom/ message Drops form on the inner wall of the ept.i.p.s. pipette tip. Liquid is dripping from the tip and/or the dispensed volume is incorrect. Cause Inner wall has not been uniformly prewet. The tip is loose or the pipette tip is poorly fitted. Pipetted too quickly. The tip is withdrawn from the liquid too quickly. Remedy Use new ept.i.p.s. pipette tip or ept.i.p.s. pipette tip. Firmly attach the pipette tips. Use ept.i.p.s. pipette tips. For 5 ml and 10 ml ep Dualfilter T.I.P.S. pipette tips, work without a protection filter in the pipette. Move the control button slowly. Slowly remove the tip from the liquid with a time delay (approx. 3 seconds). Other possible reasons for faults associated with overall operation are described in the operating instructions for the device being used. These brief instructions for use do not replace the operating instructions for the individual devices. CAUTION! CAUTION! Poor safety due to incorrect accessories and spare parts. The use of accessories and spare parts other than those recommended by Eppendorf may impair the safety, function and precision of the device. Eppendorf cannot be held liable or accept any liability for damage resulting from the use of incorrect or non-recommended accessories and spare parts or from the improper use of such equipment. Only use accessories and original spare parts recommended by Eppendorf. 10
11 11 ept.i.p.s. combinations 11 ept.i.p.s. combinations Eppendorf Reference 0.1 μl 10 μl S 0.1 μl 10 μl M 0.1 μl 20 μl M 0.5 μl 20 μl L 2 μl 20 μl 2 μl 100 μl 2 μl 200 μl 20 μl 300 μl 50 μl μl 50 μl μl 50 μl μl L 500 μl μl 100 μl μl 1 ml 10 ml GELoader Reference 1 ml 10 ml L Microloader Color dark gray medium gray medium gray light gray orange green dark green red violet turquoise light gray ept.i.p.s. ept.i.p.s. ep Dualfi lter T.I.P.S. ep Dualfi lter T.I.P.S. Fixed volume 1 μl, 2 μl, 5 μl, 10 μl medium gray 10 μl 20 μl 25 μl, 50 μl, 100 μl 200 μl, 250 μl, 500 μl, μl μl, μl, μl red Variable volume 0.1 μl 2.5 μl dark gray 0.5 μl 10 μl medium gray 2 μl 20 μl light gray 2 μl 20 μl 10 μl 100 μl 50 μl 200 μl 50 μl 250 μl 100 μl μl 500 μl μl red compatible limited volume 11
12 Eppendorf Research 0.1 μl 10 μl S 0.1 μl 10 μl M 0.1 μl 20 μl M 0.5 μl 20 μl L 2 μl 20 μl 2 μl 100 μl 2 μl 200 μl 20 μl 300 μl 50 μl μl 50 μl μl 50 μl μl L 500 μl μl 100 μl μl 1 ml 10 ml GELoader Research 1 ml 10 ml L Microloader Color dark gray medium gray medium gray light gray orange green dark green red violet turquoise light gray ept.i.p.s. ept.i.p.s. ep Dualfi lter T.I.P.S. ep Dualfi lter T.I.P.S. Fixed volume 10 μl 20 μl 25 μl, 50 μl, 100 μl 200 μl, 250 μl, 500 μl, μl Variable volume 0.1 μl 2.5 μl dark gray 0.5 μl 10 μl medium gray 2 μl 20 μl 10 μl 100 μl 20 μl 200 μl 30 μl 300 μl orange 100 μl μl 500 μl μl violet 1 ml 10 ml turquoise compatible limited volume 12
13 Eppendorf Research plus 0.1 μl 10 μl S 0.1 μl 10 μl M 0.1 μl 20 μl M 0.5 μl 20 μl L 2 μl 20 μl 2 μl 100 μl 2 μl 200 μl 20 μl 300 μl 50 μl μl 50 μl μl 50 μl μl L 500 μl μl 100 μl μl 1 ml 10 ml GELoader Research plus 1 ml 10 ml L Microloader Color dark gray medium gray medium gray light gray orange green dark green red violet turquoise light gray ept.i.p.s. ept.i.p.s. ep Dualfi lter T.I.P.S. ep Dualfi lter T.I.P.S. Fixed volume 10 μl medium gray 20 μl light gray 10 μl 20 μl 25 μl, 50 μl, 100 μl 200 μl 200 μl, 250 μl, 500 μl, μl Variable volume 0.1 μl 2.5 μl dark gray 0.5 μl 10 μl medium gray 2 μl 20 μl light gray 2 μl 20 μl 10 μl 100 μl 20 μl 200 μl 30 μl 300 μl orange 100 μl μl 500 μl μl violet 1 ml 10 ml turquoise compatible limited volume 13
14 Eppendorf Research pro 0.1 μl 10 μl S 0.1 μl 10 μl M 0.1 μl 20 μl M 0.5 μl 20 μl L 2 μl 20 μl 2 μl 100 μl 2 μl 200 μl 20 μl 300 μl 50 μl μl 50 μl μl 50 μl μl L 500 μl μl 100 μl μl 1 ml 10 ml GELoader Research pro 1 ml 10 ml L Microloader Color dark gray medium gray medium gray light gray orange green dark green red violet turquoise light gray ept.i.p.s. ep Dualfi lter T.I.P.S. ep Dualfi lter T.I.P.S. 0.5 μl 10 μl medium gray 5 μl 100 μl 15/20 μl 300 μl orange 50 μl μl 50 μl μl green 0.1/0.25 μl 5 ml violet compatible limited volume Eppendorf Xplorer 0.1 μl 10 μl S 0.1 μl 10 μl M 0.1 μl 20 μl M 0.5 μl 20 μl L 2 μl 20 μl 2 μl 100 μl 2 μl 200 μl 20 μl 300 μl 50 μl μl 50 μl μl 50 μl μl L 500 μl μl 100 μl μl 1 ml 10 ml GELoader Xplorer 1 ml 10 ml L Microloader Color dark gray medium gray medium gray light gray orange green dark green red violet turquoise light gray ept.i.p.s. ep Dualfi lter T.I.P.S. ep Dualfi lter T.I.P.S. 0.5 μl 10 μl medium gray 5 μl 100 μl 15/20 μl 300 μl orange 50 μl μl 50 μl μl green 0.1/0.25 μl 5 ml violet 0.5 ml 10 ml turquoise compatible limited volume 14
15 12 Technical data 12 Technical data 12.1 Ambient conditions Ambience Only for use in indoors. Ambient temperature 5 C 40 C Relative humidity Atmospheric pressure 10 % 95 %, non-condensing 79.5 kpa 106 kpa Usage up to a height of 2000 m above sea level 13 Transport, storage and disposal 13 Transport, storage and disposal 13.1 Storage Air temperature Relative humidity Atmospheric pressure In transport packaging -25 C 55 C 10 % 95 % 70 kpa 106 kpa Without transport packaging -5 C 45 C 10 % 95 % 70 kpa 106 kpa 15
16 Your local distributor: Eppendorf AG Hamburg Germany Tel: Fax: Eppendorf North America, Inc. 102 Motor Parkway Hauppauge, N.Y USA Tel: Toll free phone: Fax: Application Support Europe: Tel: (Preis je nach Tarif im Ausland; 9 ct/min aus dem dt. Festnetz; Mobilfunkhöchstpreis 42 ct/min) support@eppendorf.com North America: Tel: techserv@eppendorf.com Asia Pacific: Tel: support_asiapacific@eppendorf.com
ept.i.p.s. Instructions for use Version B
r N) s use for use Instructions for use Version B Copyright 2013 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior permission of the copyright owner. Eppendorf and
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